Blot hybridizationFluorescence in situ hybriSitu hybridization histochemistryComparative genomic hybriChromosomalNatural hybridizationGenomicProbesFluorescentGeneticAssayTumoursTissueSpeciesAnalysesGenesAmplificationHybridsPopulationsProbeExaminesHumansChromosome abnormalitiesFISHGene expressionMolecularAbstractStructuralDetectionRecentNucleicTissuesSlidesEvolutionaryMitochondrialInfluenceZonesEcologySecondaryConventionalMulticenterApproachGenomeAnalysisMethodsCrystallizationComplicationsHaematologicalColiThesisMutationSamplesUndertakenViruses
Blot hybridization3
- We used PCR amplification and Southern blot hybridization to detect 9 virulence factors among 40 EHEC type-strains from the STEC Center, National Food Safety and Toxicology Center, Michigan State University (East Lansing, MI, USA). (cdc.gov)
- resistance levels are control for colony dot blot hybridization usually high for broad-spectrum penicillin tests. (who.int)
- DNA was extracted using the alkaline-lysis and the production of extended-spectrum - method of Birnboim and Doly [16] and was lactamases by multidrug resistant E.coli has used in dot blot hybridization. (who.int)
Fluorescence in situ hybri2
- Comparative genomic hybridisation (CGH) is a dual fluorescence in situ hybridisation protocol capable of detecting global gains and losses of genomic regions by competitive DNA hybridisation. (bmj.com)
- In skin pathology, the recent application of fluorescence in situ hybridisation (FISH) has been demonstrated to be of use for the analysis and diagnosis of ambiguous melanocytic neoplasms of the skin. (bmj.com)
Situ hybridization histochemistry2
- We have studied synaptophysin messenger RNA in the neocortex, hippocampus and cerebellum using in situ hybridization histochemistry to see if the encoding transcript can be detected in post mortem human brain and to investigate factors which might influence its abundance. (ox.ac.uk)
- In situ hybridization histochemistry and the study of gene expression in the human brain. (ox.ac.uk)
Comparative genomic hybri3
- Comparative genomic hybridisation (CGH) is one of several molecular approaches that can be applied to ascertain the origin of extra chromosomal material. (bmj.com)
- We have used comparative genomic hybridisation to analyse 19 constitutional chromosome abnormalities detected by G band analysis, including seven deletions, five supernumerary marker chromosomes, two interstitial duplications, and five chromosomes presenting with abnormal terminal banding patterns. (bmj.com)
- Screening for chromosome imbalances was carried out using comparative genomic hybridisation (CGH) in 14 frozen samples of tumour from a series of 13 breast cancer patients with multiploid (n = 11) and hypertetraploid (n = 2) tumours. (bmj.com)
Chromosomal4
- Microarray-based comparative genomic hybridization is a method of molecular analysis that identifies chromosomal anomalies (or copy number variants) that correlate with clinical phenotypes. (nih.gov)
- Microarray-based comparative genomic hybridization identified structural chromosomal alterations responsible for the intellectual disability/developmental delay phenotype in 16% of our sample. (nih.gov)
- The overall aim of this project was to develop the technique of chromosomal in situ suppression (CISS) hybridization using whole chromosome specific libraries (chromosome painting) and to apply it to the investigation of diagnostic problems in clinical cytogenetics. (gla.ac.uk)
- Diagnosis is made by confirming a deletion in the WHS critical region (WHSCR) using conventional chromosome analysis, fluorescence in situ hybridization (FISH), or chromosomal microarray. (medscape.com)
Natural hybridization2
- Recently, a scientific team, which includes researchers from Greek institutions ( Natural History Museum of Crete-University of Crete , Institute of Marine Biology, Biotechnology and Aquaculture-HCMR , and Pelagos Cetacean Research Institute ), attempted to shed light on whether natural hybridization is the cause and understand the evolutionary processes that were responsible for the presence of these intermediate morphs. (databasefootball.com)
- There has previously been little reported on natural hybridization between L1 and L2. (frontiersin.org)
Genomic12
- Previously, a probe-selection method based on hybridisation of genomic DNA (gDNA) was developed, which enables GeneChips to be used for species that they were not designed for. (cambridge.org)
- DNA-DNA hybridization probes were made from virulence factors amplified from O157:H7 EDL933 genomic DNA. (cdc.gov)
- Another goal was to test the association between a positive microarray-based comparative genomic hybridization result and the severity of intellectual disability/developmental delay. (nih.gov)
- We want to emphasize the need to conduct microarray-based comparative genomic hybridization on all individuals with intellectual disability/developmental delay, regardless of the severity, because the degree of intellectual disability/developmental delay does not predict the diagnostic yield of microarray-based comparative genomic hybridization. (nih.gov)
- Studying the identity and genomic distribution of loci involved in ecological speciation, particularly in cases where parallel ecomorphological contrasts have arisen multiple times, is one way to address these questions. (biorxiv.org)
- Using such approaches, studies have already highlighted the prevalence of either strongly differentiated genomic 'islands' of differentiation 5 - 10 or genome-wide polygenic architectures of phenotypic differentiation and ecological speciation 11 - 15 . (biorxiv.org)
- Comparative genomic hybridization (CGH) is now often recommended to identify a greater number of subtle cytogenetic abnormalities in pervasive developmental disorder (PDD). (medscape.com)
- Population genomic sequencing of Coccidioides fungi reveals recent hybridization and transposon control. (broadinstitute.org)
- This study highlights the importance of population genomic data for detecting subtle but potentially important phenomena such as introgression. (broadinstitute.org)
- Routine G-banded karyotype, array-based comparative genomic hybridization, and fluorescence in situ hybridization analyses were used to characterize the cytogenetic abnormality in the patient's bone marrow. (hindawi.com)
- This case study reports a rare double minute chromosome via FLT3 amplification in CMML by using array-based comparative genomic hybridization and fluorescence in situ hybridization analyses. (hindawi.com)
- Array comparative genomic hybridization (CGH) was performed following the standard protocol provided by Agilent Technologies (Agilent Technologies, Santa Clara, CA, United States). (hindawi.com)
Probes4
- Fluorescent in Situ Hybridization (FISH) Probe refers to a sub-atomic cytogenetic technique that uses fluorescent probes to visualize genetic materials. (researchandmarkets.com)
- Initially to gain experience with non-isotopic in situ hybridization, repetitive target probes DYS59 (GMY10) and DYS58 (GMGY7) were used. (gla.ac.uk)
- Therefore, in our study, E. coli complex, multi-factorial mechanism involv- isolates from diarrhoeal cases were ana- ing a large number of virulence factors that lysed using specific DNA probes for genes vary with pathotype. (who.int)
- The results of MSRF were confirmed by southern hybridization analysis using the aberrantly methylated DNA fragments as the probes. (cdc.gov)
Fluorescent5
- Delve into the vibrant sector of the global fluorescent in situ hybridization (FISH) probe market, a domain exhibiting unprecedented growth powered by escalating demand and scientific progression. (researchandmarkets.com)
- The global fluorescent in situ hybridization (FISH) probe market size reached US$ 816 Million in 2022. (researchandmarkets.com)
- The report provides an analysis of the key trends in each sub-segment of the global fluorescent in situ hybridization (FISH) probe market report, along with forecasts at the global, regional and country level from 2023-2028. (researchandmarkets.com)
- The hybridization images were acquired under a fluorescent microscope. (scirp.org)
- Cytogenetic and fluorescent in situ hybridization studies were normal. (who.int)
Genetic9
- However, the constraints to rapid diversification and how they are sometimes overcome, particularly the relative roles of genetic architecture and hybridization, remain unclear. (biorxiv.org)
- It also provides a useful focal point for studying the mechanisms of microevolution such as gene flow, genetic drift, and selection. (uoguelph.ca)
- In a new study published today in Scientific Reports, however, Graham L Banes and Linda Vigilant of the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany, caution that such reintroductions can act as a form of genetic translocation. (mpg.de)
- Advances in morphological and genetic studies have since revealed two species of orang-utan, however, on the islands of Borneo and Sumatra. (mpg.de)
- Our data confirm earlier microsatellite-based findings that these species are genetically differentiated, but our population genomics approach reveals that hybridization and genetic introgression have recently occurred between the two species. (broadinstitute.org)
- Cases of hybridization that lead to viable offspring that are able to reproduce have implications on the genetic variability within individuals upon which natural selection can operate and, at a broader scale, on fundamental evolutionary processes such as speciation. (databasefootball.com)
- In order to accomplish that, they collected skin samples from individuals of the two species of dolphins as well as from the intermediate morphs and conducted a DNA-based study by means of two types of genetic markers (bi-parentally inherited high resolving microsatellite DNA markers and maternally transmitted mitochondrial DNA sequences) serving as two independent sources of information. (databasefootball.com)
- Utilizing innovative technologies including channel rhodopsin-based circuit manipulation and genetic manipulation in rodents, recent studies have begun to answer these central questions. (frontiersin.org)
- Genetic interactions between avian H5N1 influenza and human seasonal influenza viruses have the potential to create hybrid strains combining the virulence of bird flu with the pandemic ability of H1N1, according to a new study. (healthnewstrack.com)
Assay4
- Protein crystallization is a key assay for structural studies of proteins. (gracebio.com)
- 1,2 A novel probe hybridization quantitative polymerase chain reaction (qPCR) assay targeting the cytochrome oxidase I (COI) gene of P. andersoni was developed. (vin.com)
- Some toxins show a encoding various toxins using hybridization strong association with specific pathotypes assay. (who.int)
- The hybridization assay using dot blot leicher & Schuell). (who.int)
Tumours1
- CGH has been widely applied to detect gains and losses of DNA sequences on specific chromosomes in the study of solid tumours, 1 but has not been extensively used to study unbalanced constitutional karyotypes. (bmj.com)
Tissue3
- Techniques based on immunohistochemistry have thus also been widely used to study the expression of cytokines within the synovial tissue in attempts to determine true local production. (bmj.com)
- 6-16 However, most previous studies based on immunohistochemical staining of intra-articular cytokine production in RA have been performed on acetone fixed tissue, a procedure which does not readily allow subsequent discrimination of cytokine producing cells and cytokine binding cells. (bmj.com)
- The formalin-fixed, paraffin-embedded (FFPE) tissue sections with a 5-μm thickness were cut for subsequent miR-637 in situ hybridization (ISH). (scirp.org)
Species10
- Affymetrix GeneChip microarrays are a powerful tool to study global gene expression profiles and have been used on many species. (cambridge.org)
- This approach can greatly increase the number of potential annotated genes that can be studied beyond that which is currently available, particularly for species that do not have comprehensively characterised genomes. (cambridge.org)
- This same method also has the potential to allow the study of other species where species-specific microarrays are not available or whose genomes have not been comprehensively characterised. (cambridge.org)
- We have explored these issues through studies on endangered species, effective population size, metapopulation dynamics, the effects of drift on mating system evolution, inbreeding depression, and the mechanisms by which hybridization causes extirpation of rare species. (uoguelph.ca)
- This task becomes even more laborious when studying species that are difficult to observe, such as marine mammals. (databasefootball.com)
- The Gulf of Corinth (GOC) constitutes a unique and very interesting area where three sympatric dolphin species form permanent mixed-species groups, providing excellent opportunities for interspecific sexual interaction and the potential for hybridization. (databasefootball.com)
- This phenomenon could either be the result of interspecific hybridization between the two aforementioned species or might represent another incident of the high morphological variability in the pigmentation pattern of the striped dolphin. (databasefootball.com)
- The intermediate morphs also carried DNA of both parental species (in respect of the genome that is inherited by both parents,) i.e., they were admixed, displaying unique variation indicative of recent hybridization events between the two species. (databasefootball.com)
- The question of taxonomic recognition for 'races' of hybrid ancestry between valid species is studied, and the conclusion is reached that morphological criteria and not lines of descent should dictate whether such races are valid or not. (amnh.org)
- The problem of the assignment of such a race to a species group is studied. (amnh.org)
Analyses2
- The aim of the present study was to apply a clinical score previously designated by de Vries to 329 patients with intellectual disability/developmental disorder (intellectual disability/developmental delay) referred to our tertiary center and to see whether the clinical factors are associated with a positive outcome of aCGH analyses. (nih.gov)
- In both cases we are using comparative analyses, manipulative experiments and multi-generational selection studies to understand the evolution of these traits. (uoguelph.ca)
Genes4
- Besides demonstrating that the probe selection method can be used to study the ovine transcriptome, the benefits of this approach are that it can greatly increase the number of annotated and novel genes that can be studied beyond those currently possible using ovine- or bovine-specific microarrays. (cambridge.org)
- The increased virulence seen in the new study seems to arise from one of the eight genes in the viral genome, called PB2, which is known to affect how well the bird flu virus grows in mammalian hosts, including humans. (healthnewstrack.com)
- The study also proposed another possible mechanism of FLT3 genes in leukemogenesis. (hindawi.com)
- We have undertaken studies investigation aberrant DNA methylation resulting in changes in the expression of cancer-related genes as a possible epigenetic mechanism for Cd carcinogenesis. (cdc.gov)
Amplification4
- We compared PCR amplification of 9 enterohemorrhagic Escherichia coli virulence factors among 40 isolates (21 O/H antigenicity classes) with DNA hybridization. (cdc.gov)
- This study addresses the potential for a broad and well-characterized set of control strains relative to virulence factor amplification and confirmed by Southern hybridization. (cdc.gov)
- An ultrasensitive sandwich-type electrochemical biosensor for DNA detection is developed based on spherical silicon dioxide/molybdenum selenide (SiO2@MoSe2) and graphene oxide-gold nanoparticles (GO-AuNPs) hybrids as carrier triggered Hybridization Chain Reaction (HCR) coupling with multi-signal amplification. (americanelements.com)
- The current study attempts to demonstrate the existence of double minute chromosomes via FLT3 gene amplification in a patient diagnosed with chronic myelomonocytic leukemia (CMML). (hindawi.com)
Hybrids1
- Two dogs out of the 60 or so we studied were inferred to be first generation hybrids," he added. (sciencedaily.com)
Populations2
- This phenomenon is called hybridization and is far more common in natural populations than previously thought, as suggested by the findings of numerous scientific studies. (databasefootball.com)
- Studying hybridization in natural populations offers a unique opportunity for the study of incipient evolution. (databasefootball.com)
Probe5
- In this study, we demonstrate that gDNA-based probe selection on the Affymetrix Human U133+2 GeneChip array can be used to study gene expression profiles in sheep tissues. (cambridge.org)
- This provided experience in labelling of probe with biotin, hybridization and detection conditions (alkaline phosphatase detection) and analysis of results. (gla.ac.uk)
- The maximum final concentration of the DNA mixture per slide was 10ug/10ul and increasing the ratio of the probe and/or the unlabelled DNA did not improve either the quality of suppression or the hybridization signal. (gla.ac.uk)
- Prebanding of slides prior to hybridization did not affect the target chromosomes, however, incomplete destaining did hinder probe penetration and interfere with counterstaining. (gla.ac.uk)
- In general, the technical difficulties were related to either probe preparation, poor hybridization, non-homogeneous painting or high background but with modifications of the parameters as detailed above the method was shown to be reliable and reproducible. (gla.ac.uk)
Examines1
- This study examines the expression of mu, delta, and kappa receptor mRNAs in the rat brain and spinal cord using in situ hybridization techniques. (nih.gov)
Humans1
- Known causes of haematological al y have not indicated an increased In 1974, IARC concluded that studies cancers in humans are ionizing ra- risk of AML. (who.int)
Chromosome abnormalities2
- Others have applied CGH to the study of constitutional chromosome abnormalities detected postnatally. (bmj.com)
- Three-color in situ hybridization was applied to the study of numerical chromosome abnormalities as occur in human solid tumors. (nih.gov)
FISH2
- High-resolution cytogenetic studies with FISH are used to test for 15q duplication, which has been found in 3%-5% of individuals with autism. (medscape.com)
- Population in Alabama was intentionally stocked by a federal fish hatchery during studies (Mettee et al. (usgs.gov)
Gene expression1
- Synaptophysin gene expression in human brain: a quantitative in situ hybridization and immunocytochemical study. (ox.ac.uk)
Molecular2
- G band cytogenetic analysis often leads to the discovery of unbalanced karyotypes that require further characterisation by molecular cytogenetic studies. (bmj.com)
- The resources should include immunohistochemical and molecular studies, obtaining expert opinion and rebiopsy. (medscape.com)
Abstract1
- ABSTRACT We studied 21 patients with Sanjad-Sakati syndrome (SSS) from 16 families. (who.int)
Structural1
- Bryndorf et al 2 applied CGH to the study of unbalanced karyotypes in prenatal cases, including seven cases of unbalanced structural abnormalities and four cases of simple aneuploidies. (bmj.com)
Detection2
- Detection of synaptophysin messenger RNA broadens the range of methods by which synaptic protein gene products can be studied and used as markers of synaptic density and synaptic involvement during physiological and pathological processes in human brain. (ox.ac.uk)
- Different computational methodologies for anomaly detection has been studied in the past. (researchgate.net)
Recent7
- Hybridization of wolves with shepherd dogs in the Caucasus region might be more common, and more recent, than previously thought, according to new research. (sciencedaily.com)
- Dog owners in the Caucasus Mountains of Georgia might want to consider penning up their dogs more often: hybridization of wolves with shepherd dogs might be more common, and more recent, than previously thought, according to a recently published study in the Journal of Heredity . (sciencedaily.com)
- We expected to identify some individuals with hybrid ancestry, but it was quite surprising that recent hybrid ancestry was found in every tenth wolf and every tenth shepherd dog," said study co-author Tarkhnishvili. (sciencedaily.com)
- The study also found that about a third of the dogs sampled shared relatively recent maternal ancestry with local wolves, not with wolves domesticated in the Far East, where most experts believe dogs were first domesticated. (sciencedaily.com)
- Recent studies have shown the involvement of oxidative stress and mitochondrial activity in AGA. (nature.com)
- Recent studies showed that treatment of hair follicles by agonistic modulators induced entry into catagen with an anti-inflammatory mechanism 20 . (nature.com)
- However, agents such as formaldehyde and term studies of experimental animals recent mechanistic data, as well as benzene. (who.int)
Nucleic1
- A method is described for visualizing three nucleic acid sequences simultaneously by in situ hybridization using a new blue immunofluorescent label, amino methyl coumarin acetic acid (AMCA), in combination with green and red fluorescing FITC and TRITC. (nih.gov)
Tissues1
- We assessed miR-637 expression in 98 and 16 gliomas and non-tumoral brain tissues, respectively, using in situ hybridization. (scirp.org)
Slides1
- It was found that refixing of slides (either new or old slides) in methanol:acetic acid (3:1) before denaturation tended to improve the hybridization result as well as reducing background signal. (gla.ac.uk)
Evolutionary1
- The Boulder Chickadee Study is a collaborative, long term effort between Kathryn Grabenstein, a PhD candidate in ecology and evolutionary biology at the University of Colorado Boulder, and her thesis advisor, Scott Taylor, to examine hybridization. (colorado.edu)
Mitochondrial1
- The research team examined maternally-inherited DNA (mitochondrial DNA) and microsatellite markers to study hybridization rates. (sciencedaily.com)
Influence2
- There are several influence factors which determinate the way of hybridization, which practice is applied and which not. (wikipedia.org)
- This study highlights the influence of habitat availability on hybrid zone movement. (lu.se)
Zones2
- The hypothesis is advanced that, as a result of the removal of the mountain forests by man, these two morphologically very different groups were brought into hybrid contact and that zones of hybridization formed along the artificial forest edges in the midst of what had formerly been pure mountain forest. (amnh.org)
- Theoretical and empirical studies suggest that the structure and position of hybrid zones can change over time. (lu.se)
Ecology2
- I am currently a member of the Canadian Society of Ecology and Evolution, Society for the Study of Evolution, American Association for Higher Education and Botanical Society of America. (uoguelph.ca)
- The ecology of hybridization in New Guinea honeyeaters (Aves). (amnh.org)
Secondary1
- These results highlight the role of both genome architecture and secondary contact with hybridization in fuelling adaptive radiation. (biorxiv.org)
Conventional1
- Constitutional chromosome studies involving conventional GTG band cytogenetic analysis, however, often show unbalanced karyotypes which cannot be fully defined because the extra material is too small to have a recognisable banding pattern. (bmj.com)
Multicenter1
- Highly active antiretroviral therapy (HAART) improves survival in HIV-associated Hodgkin's disease: results of a multicenter study. (medscape.com)
Approach2
- Here, we apply a de novo hybridization approach based on a breed-centric methodology to elucidate novel neuraminidase inhibitors. (bvsalud.org)
- We answer the purpose by combining research on hybrid organization with research on strategy and boundary spanning activities and by analyzing an organization's hybridizing process, using a case study approach. (lu.se)
Genome1
- A genome-wide association study of Hodgkin's lymphoma identifies new susceptibility loci at 2p16.1 (REL), 8q24.21 and 10p14 (GATA3). (medscape.com)
Analysis2
- In this study, we provide further in-depth analysis to findings from transcriptome profiling of hair bulbs from AGA patients identified previously 24 . (nature.com)
- Through qualitative interviews with the editors at each of the publications, as well as through a two-month framing analysis of the coverage at the outlets, this study explores how multiple public sphericules can be bonding agents, building a sense of cohesion within a cultural community, while at the same time bridging that cultural community with the larger communities in which they live. (concordia.ca)
Methods1
- Hybridization comprises the fusion of country- and culture-specific election campaigning methods with contemporary styles and techniques. (wikipedia.org)
Crystallization1
- Hybridization at 42-45C showed crystallization and heavy background deposits. (gla.ac.uk)
Complications1
- Children with DCM are at a particular risk for complications during cardiac catheterization studies and angiography. (medscape.com)
Haematological1
- 1987. Exposure to benzene in Turkey between 1983 and 1985: A haematological study on 231 workers. (cdc.gov)
Coli1
- Another class of heat-stable toxin, entero- to standard procedures [15] and used them aggregative E. coli heat-stable enterotoxin for this study. (who.int)
Thesis1
- Scholars in the areas of diasporic studies, South Asian studies, and counterpublic formation inform this thesis. (concordia.ca)
Mutation1
- In a Brazilian study, neonatal screening for the TP53 R337H mutation, which is prevalent in the region, identified 461 (0.27%) carriers among 171,649 newborns who were screened. (oncolink.org)
Samples1
- When you have uploaded and imported your raw data, collected and registered all information and annotations about samples, hybridizations, and other items, it is time to collect everything in an experiment. (lu.se)
Undertaken1
- The study was undertaken as part of Dr. Kopaliani's work exploring human-wolf conflict in Georgia. (sciencedaily.com)
Viruses1
- Before the current study, hybrid viruses generated in lab studies had always been less virulent than parent strains. (healthnewstrack.com)