Loading...
  • bacteria
  • AG showed inhibitory effects against the initial adhesion of S. epidermidis at concentrations of 1 000,100, 10 mg x L(-1), respectively,and inhibited metabolism of biofilm bacteria at the concentration of 31.25 mg x L(-1), and exhibited significantly inhibition against the biofilm morphology at the concentration of 250 mg x L(-1), while did not display inhibition against PIA formation at the concentration of 10 mg x L(-1). (sigmaaldrich.com)
  • vitro
  • S. epidermidis biofilms were set up in vitro, erythromycin was acted as the positive control agent, XTT reduction assay was used to evaluate AG on the initial adhesion of S. epidermidis and bacterial metabolism within biofilm, microscope was applied to observe biofilm morphology, and Congo red assay was used to detect polysacchatide interc-ellular adhesion (PIA)formation when exposed to AG. (sigmaaldrich.com)
  • planktonic cells
  • In conclusion, we recommend the use of the geometric mean of the relative expression values from the five housekeeping genes (ACT1, PMA1, RIP, RPP2B and LSC2) for normalization, when analysing differences in gene expression levels between C. albicans biofilm cells and planktonic cells. (nih.gov)
  • Stability ranking of the housekeeping genes in all samples (biofilms and planktonic cells). (nih.gov)
  • The pharmacodynamic properties of all three antifungals have been described in studies using free-floating (planktonic) cells ( 2 , 6 , 7 ). (asm.org)
  • With some exceptions, biofilm cells do not grow better than planktonic cells in the presence of a broad range of antimicrobials. (asm.org)
  • Unlike planktonic cells of P. aeruginosa that were very sensitive to killing by 50 mM H 2 O 2 , biofilm cells that actually had lower levels of catalase (KatA) were effectively protected ( 18 , 24 ). (asm.org)
  • natural biofilm
  • These idealized conditions are often not realized in practice, and the explicit consideration of additional elements of natural biofilm environments has recently begun to reveal important new features of this microbial lifestyle ( 4 ). (asm.org)
  • oral biofilm
  • We aimed to compare cariogenic and microbiological effects of soluble starch in both a standard animal model and an oral biofilm system, and to assess the possible substitution of the animal model. (uzh.ch)
  • Understanding the ecology of oral biofilm communities, and how different species communicate within a given host, will inform new strategies for treatment and prevention of oral diseases. (salford.ac.uk)
  • albicans biofilms
  • Development and evaluation of different normalization strategies for gene expression studies in Candida albicans biofilms by real-time PCR. (nih.gov)
  • Candida albicans biofilms are commonly found on indwelling medical devices. (nih.gov)
  • (nih.gov)
  • Our data indicate that five genes are necessary for accurate and reliable normalization of gene expression data in C. albicans biofilms. (nih.gov)
  • Using different normalization strategies, we found a significant upregulation of the ALS1 gene and downregulation of the ALS3 gene in C. albicans biofilms grown on silicone disks in a continous flow system, the CDC reactor (Centre for Disease Control), for 24 hours. (nih.gov)
  • Validation of the normalization strategies described above showed that the ALS1 gene is overexpressed and the ALS3 gene is underexpressed in C. albicans biofilms grown on silicone in the CDC reactor for 24 hours. (nih.gov)
  • We have examined the in vitro activities of fluconazole, amphotericin B, and caspofungin against Candida albicans biofilms by time-kill methodology. (asm.org)
  • Log plots of decreased viability (percent) in biofilms treated with fluconazole versus time for each isolate are presented in Fig. 2A and B. Fluconazole challenge of preformed C. albicans biofilms showed minimal activity at all concentrations tested over the 48-h period, with a maximal 30% decrease in biofilm activity exhibited after 48 h. (asm.org)
  • species
  • The objectives of this study were to determine whether human follicular fluid can support bacterial growth over time, whether the steroid hormones estradiol and progesterone (present at high levels within follicular fluid) contribute to the in vitro growth of bacterial species, and whether species isolated from follicular fluid form biofilms. (plos.org)
  • Methods and Results: Six-species biofilms were grown anaerobically on enamel discs in saliva and medium with glucose/sucrose, starch (average molecular weight of 5000, average polymerization grade of 31), or mixtures thereof. (uzh.ch)
  • The oral cavity supports a complex and finely balanced consortium of microbial species, many of which cooperate within structured biofilms. (salford.ac.uk)
  • resistant
  • Interested in finding out that Salmonella might have something special about it that makes it better at surviving in the environment of a food processing facility, we asked was it better at forming a dense biofilm, or was it more resistant to disinfectants than other Salmonella ? (healthcanal.com)
  • Chuanwu Xi, PhD, Assistant Professor in the Department of Environmental Health at the University of Michigan and his research team found that treated steam from a novel steam disinfection system utilizing TANCS® technology rapidly kills highly resistant biofilms with greater than 99.95 % killing efficiency in a 3 second treatment, and to a non-detectable level in a less than 10 second treatment. (prweb.com)
  • pathogen
  • however, rather than studying the virulence of the pathogen, Dalia and researchers in his lab will leverage this well-established model system and the genetic tools they have developed to characterize biofilms in a physiologically relevant context. (indiana.edu)
  • Our lab hopes to use what we've learned about H. influenzae biofilms to identify vaccine targets as well as improve existing methods of therapeutic treatment for the diseases of the respiratory tract caused by this prevalent pathogen. (phys.org)
  • Close association of the pathogen Helicobacter pylori in drinking water biofilms has been suggested. (soton.ac.uk)
  • Novel technology peptide nucleic acid (PNA) molecular probes were used to detect and locate the pathogen in the biofilms. (soton.ac.uk)
  • The pathogen was tracked in the biofilms using epifluorescence microscopy and episcopic differential interference contrast microscopy. (soton.ac.uk)
  • Wound Healing
  • We have established and validated a highly quantitative, reproducible in vivo biofilm model, while providing evidence that the biofilm phenotype specifically contributes to profound cutaneous wound healing impairment. (dtic.mil)
  • 2017
  • Darla Goeres, associate research professor of chemical and biological engineering in the Center for Biofilm Engineering at Montana State University, helps Lindsey Lorenz, a research assistant, drain a beer draught line on Thursday, June 1, 2017, in Bozeman, Montana, in preparation to test the tubing for biofilm build up. (montana.edu)
  • influenzae
  • S. pneumoniae and H. influenzae were not good biofilm formers in pure culture, but these pathogens showed some attachment when they were combined in mixed culture. (researchgateway.ac.nz)
  • The novel mechanism by which nontypeable H. influenzae releases DNA through an inner membrane pore complex that partners with another complex in the outer membrane allows DNA to be ejected out into the biofilm , via a previously unidentified process. (phys.org)
  • viability
  • An initial set of experiments was performed to validate the XTT-colorimetric readings for determining cell viability compared to total viable cell counts in caspofungin-treated biofilms. (asm.org)
  • mechanisms
  • Ankur Dalia, an assistant professor in the Department of Biology, and his lab study the basic biology and mechanisms of biofilms using Vibrio cholerae as a model system. (indiana.edu)
  • Dalia was awarded $1,894,717 earlier this year for his grant application titled "Characterizing the basic biology and mechanisms of biofilms in Vibrio cholerae " through a Maximizing Investigators' Research Award for Early Stage Investigators (R35). (indiana.edu)
  • Dalia aims to define the mechanisms of initial adherence to chitin, DNA uptake and integration during natural transformation, and metabolism during growth in chitin biofilms and in the mammalian host following transmission. (indiana.edu)
  • antibiotic
  • Second, growth in chitin biofilms induces natural transformation, a conserved mechanism of horizontal gene transfer that can promote the acquisition of antibiotic resistance genes and novel virulence factors. (indiana.edu)
  • Retarded diffusion will decrease the concentration of the antibiotic entering the biofilm, helping an enzyme like β-lactamase destroy the incoming antibiotic. (asm.org)
  • It can be expected that any mechanism of antibiotic destruction or modification (like acetylation of aminoglycosides) will be especially effective when coupled with a diffusion barrier of the biofilm. (asm.org)
  • antimicrobial
  • Previous studies have shown that whilst human follicular fluid has antimicrobial properties, it is still capable of supporting microbial growth in vitro , . (plos.org)
  • At the same time, restricted diffusion can protect the biofilm from a degradable antimicrobial. (asm.org)
  • degradation
  • These biofilms then play a fundamental role in determining the degradation of the particles and, ultimately, the fate of carbon in the particles, with a direct impact on the flux of carbon from the upper to the deep ocean ( 9 ). (asm.org)
  • The authors demonstrated that the degradation of these polymers, and the concomitant dissipation of the biofilm, causes a decrease in the xCELLigence biosensor signal that is both time- and dose-dependent. (news-medical.net)
  • esters
  • Wax esters in the hydrocarbon fraction possibly suggested the presence of localized anaerobic zones within the biofilms, where the microeukaryotes synthesized the esters by an unusual reverse β-oxidation pathway. (iastate.edu)
  • microtiter
  • Biofilms of C. albicans strains SC5314 and 3153A were formed as previously described by our group by using microtiter plates ( 15 , 16 ). (asm.org)
  • For plate counts, triplicate biofilms were removed from microtiter wells by scraping the biofilms and vortexed vigorously to disperse the cells. (asm.org)
  • Because each well of a xCELLigence microtiter plate can acquire thousands of data points, each individual well yields a complete time course for biofilm deposition or dissipation, greatly reducing the number of wells/plates needed and the total workload. (news-medical.net)
  • vivo
  • Given that most studies to date rely on in vitro or observational clinical data, our aim was to develop a novel, quantitative animal model enabling further investigation of the biofilm hypothesis in vivo. (dtic.mil)
  • Significance and Impact of the Study: By leading to the same scientific evidence as its in vivo counterpart, the described in vitro biofilm system provides an interesting and valuable tool in the quest to reduce experimentation with animals. (uzh.ch)
  • This review discusses the most recent in vitro and in vivo studies of interspecies interactions within polymicrobial oral biofilms. (salford.ac.uk)
  • synergistic
  • In the ongoing effort to develop better biofilm therapeutics, Gutierrez and colleagues suggested that the synergistic effects of combining phage enzymes with different substrate specificities should be a key area of focus. (news-medical.net)
  • chronic
  • This motivated him to study not just prevention and treatment of chronic wounds, but to delve into the rich history of wound care over the centuries. (woundsource.com)
  • Thus, biofilms may promote cancer in the colon by inducing chronic inflammation and associated cell proliferation. (eurekalert.org)
  • A growing body of evidence suggests that in addition to hypoxia, ischemia-reperfusion injury, and intrinsic host factors, bacterial biofilms represent a fourth major pillar in chronic wound pathogenesis. (dtic.mil)
  • genes
  • The eight genes tested in this study are ranked according to their expression stability (from most stable to least stable) as follows: ACT1 (beta-actin)/PMA1 (adenosine triphosphatase), RIP (ubiquinol cytochrome-c reductase complex component), RPP2B (cytosolic ribosomal acidic protein P2B), LSC2 (succinyl-CoA synthetase beta-subunit fragment), IMH3 (inosine-5'-monophosphate dehydrogenase fragment), CPA1 (carbamoyl-phosphate synthethase small subunit) and GAPDH (glyceraldehyde-3-phosphate dehydrogenase). (nih.gov)
  • Stability ranking of the housekeeping genes in biofilms. (nih.gov)
  • These include restricted penetration of antimicrobials into a biofilm, decreased growth rate, and expression of possible resistance genes. (asm.org)
  • caries
  • To assess caries incidence in rats, the same microbiota as in the biofilm experiments were applied. (uzh.ch)
  • restorative
  • Indirect dental restorative composite resins were found to be less prone to biofilm adhesion than direct composite resins. (ovid.com)
  • novel
  • A research study identifying novel bacterial physiology in the creation of biofilms by Nationwide Children's Hospital scientists has been published in the current issue of Proceedings of the National Academy of Sciences ( PNAS ). (phys.org)
  • The study, reported in Cell Metabolism on May 7, 2015, used novel metabolomic technologies to reveal molecular evidence suggesting a vicious circle in which cancerous changes in colon cells promote the growth of bacterial conglomerations called biofilms, and biofilms in turn promote cancer development. (eurekalert.org)
  • resistance
  • By this conventional criterion, biofilm cells do not necessarily show increased resistance. (asm.org)
  • One can find a list of factors considered to be responsible for biofilm resistance in papers and recent reviews on the subject ( 15 , 22 , 36 ). (asm.org)
  • Killing
  • The research shows that a lot of the time, the disinfectant may add very little, if anything, to good cleaning and appropriate food handling practices and that there is a need for more research to define better methods for killing Salmonella biofilm. (healthcanal.com)
  • The MondoVap with TANCS technology uses only tap water to generate steam for cleaning and killing biofilms without leaving chemical residue. (prweb.com)
  • Killing of biofilm cells was suboptimal at therapeutic concentrations of amphotericin B. Caspofungin displayed the most effective pharmacokinetic properties, with ≥99% killing at physiological concentrations. (asm.org)
  • Killing biofilm - mission impossible? (niom.no)
  • experimental
  • Biofilm research has traditionally aimed to recreate quiescent experimental conditions in topographically simple environments (often straight flat surfaces) where physical conditions are homogeneous and temporal variations in the external environment are generally suppressed by design ( 10 ). (asm.org)
  • rapidly
  • Following ingestion of a chitin biofilm, V. cholerae must rapidly alter its metabolism from growth on chitin to competing with the intestinal microbiota for the carbon sources available within its infected host. (indiana.edu)
  • physiology
  • The trans/cis ratio of monounsaturated fatty acids can be a good indicator of the physiology of the biofilms, indicating whether it is attached or floating, and further depicting the nutrient conditions in which they thrive. (iastate.edu)
  • metabolism
  • In preliminary data, they demonstrate that this tool is well poised to dissect the metabolism of V. cholerae in chitin biofilms and in the mammalian host. (indiana.edu)
  • fundamental
  • Pili are broadly conserved, and the Dalia lab's studies will address fundamental and long-standing questions about these structures that should be applicable to many bacterial pathogens. (indiana.edu)
  • Additionally, these particles represent alternative models for biofilm studies that have fundamental differences from traditional systems, both due to the shape and three-dimensional nature of the substrate, and because the substrate acts as both the surface for attachment and the source of nutrients. (asm.org)
  • cells
  • To validate our normalization strategies we determined differences in ALS1 and ALS3 expression levels between C. albicans biofilm cells and their planktonic counterparts. (nih.gov)
  • In other words, the cancerous cells and the biofilms both seemed to be contributing to its overproduction. (eurekalert.org)
  • The dry steam generated can easily break the barrier of biofilm structures and kill cells inside the biofilms. (prweb.com)
  • Why, then, do biofilm cells not die? (asm.org)
  • concentration
  • The dose-response curves generated by this approach made it possible to accurately calculate key parameters such as the MBEC50 (the minimum biofilm eradicating concentration that removes 50% of the biofilm) for each treatment. (news-medical.net)
  • methods
  • Instead, Goeres and her collaborators at the Standard Biofilm Methods Laboratory in MSU's Center for Biofilm Engineering use the brew to study how to improve the taste of beer everywhere, by finding ways to better remove taste-altering biofilms from the plastic lines that carry beer from keg to tap. (montana.edu)