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Microscopy, ElectronMicroscopy, Electron, ScanningImmunoassayMicroscopy, Electron, TransmissionMicroscopy, ImmunoelectronMicroscopyReagent Kits, DiagnosticElectronsElectron TransportMicroscopy, ConfocalImmunohistochemistryMicroscopy, FluorescenceImmunoenzyme TechniquesSensitivity and SpecificityMicroscopy, Electron, Scanning TransmissionMicroscopy, Atomic ForceMolecular Sequence DataCryoelectron MicroscopyElectron Spin Resonance SpectroscopyFreeze FracturingElectron Probe MicroanalysisMicroscopy, Phase-ContrastCell MembraneStaining and LabelingMicroscopy, Fluorescence, MultiphotonMicroscopy, Scanning TunnelingNegative StainingMicroscopy, PolarizationMicroscopy, InterferenceHistocytochemistryMicroscopy, VideoSurface PropertiesFreeze EtchingFluorescent Antibody TechniqueParticle SizeMicroscopy, Energy-Filtering Transmission ElectronMicrotomyRabbitsCells, CulturedCorrosion CastingOxidation-ReductionCell LineCytoplasmTime FactorsModels, MolecularHistological TechniquesGoldEpitheliumPhosphotungstic AcidCytoplasmic GranulesOrganoidsProtein ConformationAmino Acid SequenceX-Ray DiffractionKineticsCattleFluorescent DyesInclusion Bodies, ViralEscherichia coliImage Processing, Computer-AssistedBacterial ProteinsMitochondriaFreeze SubstitutionOsmiumCell NucleusActinsCell WallMembrane ProteinsMicroscopy, Scanning ProbeNanoparticlesProtein BindingGlutaralInclusion BodiesImaging, Three-DimensionalOrganellesTemperatureCryoultramicrotomyElectron Transport Complex IVMutationElectrophoresis, Polyacrylamide GelTissue EmbeddingVirionCytoskeletonMicrotubulesBasement MembraneMolecular WeightSpectroscopy, Fourier Transform InfraredMaterials TestingElectron Microscope TomographyMacromolecular SubstancesCentrifugation, Density GradientHydrogen-Ion ConcentrationPolymersFreezingCollagenSilverVacuolesLightModels, BiologicalCrystallization
UltrastructuralPneumoniaeCharacterizeSituConfocalMicroscopeScanningImmunofluorescenceNeuronsSynapsesLocalizationMicroscopicTransmissionBiochemicalEffectivenessHumansMiceSecondarySubstancesInfectionsCellsInitiateDemonstrateGeneticHumanPrevalenceLightAntibodiesAnalysisResultsVacuumMolecularBrainDiseasesWesternProcessSamplesTechniquesDynamicsCommunityStrainPresent studyEarlyContentAnimal
Ultrastructural3
  • This study aimed to apply electron and immunoelectron microscopy (EM and IEM) approaches for ultrastructural study of cellular processes and to quantify distributions of relevant proteins across subcellular compartments under chronic diseases such as HCV and DFUs or after HPP therapy of DFUs. (uclv.cu)
  • Our approach revealed the value of quantifying subcellular localization of proteins at ultrastructural level to study intracellular dynamics of key signaling pathways in a relevant therapeutic setting. (uclv.cu)
  • Ultrastructural study of leprosy granuloma and blood vessels using immunoelectron-microscopy and molecular methods. (icmr.nic.in)
Pneumoniae2
  • Our findings hold such great implications as to how inflammation in the AD brain may be triggered by infection with C. pneumoniae that we must demand that studies to replicate and/or validate our first report should be performed with the rigor and comparable techniques that will provide data that can truly be compared and analyzed. (alzforum.org)
  • Our study was designed to know the prevalence of M. pneumoniae in children with community‑acquired pneumonia and the involvement in the cytoadherence to the respiratory epithelium by M. pneumoniae using electron microscopy and immuno‑gold labeling technique. (ajol.info)
Characterize2
  • The detection level accomplished in this study should help not only to further characterize anti-LOS antibodies in blood and body fluids but also to analyze carbohydrate structures that are recognized by them. (go.jp)
  • Confocal microscopy with lectin-gold labeling on ultrathin sections, scanning electron microscopy, and laminin immuno-histochemistry were combined to characterize the cellular composition and the structure of the dome-associated crypts and the dome epithelium. (nih.gov)
Situ3
  • In situ hybridization and immunohistochemical studies have shown that Purkinje cells (PCs), the output neurons of the cerebellar cortex, are the neuron type with the highest levels of GABA B receptors (Bowery et al. (springer.com)
  • Immuno-electronmicroscopical studies of purified chitosomes, gained from a yeast strain carrying a chitin-synthase gene fused to that for GFP (green-fluorescence protein), has led to the in situ localization of chitin-synthase-GFP molecules within chitosomes. (mdpi.com)
  • In this study, we sought to determine the effect of DHA on cell surface microdomain organization in situ. (tamu.edu)
Confocal1
Microscope3
  • Materials to be viewed under an electron microscope may require processing to produce a suitable sample. (lu.se)
  • There are two types of electron microscopes: (1) Transmission electron microscope (TEM), and (2) Scanning electron microscope (SEM). (lu.se)
  • The microscope is also equipped with a retractable STEM detector (Scanning Transmission Electron Microscopy) that allows us to image thin, electron transparent samples with sub nanometer resolution. (lu.se)
Scanning3
  • In addition, the sites of lymphocyte invasion into the dome epithelium were studied after removal of the epithelium using scanning electron microscopy. (nih.gov)
  • A scanning electron microscopy image of SARS-CoV-2 obtained from immuno-compromised patient's nasopharyngeal swab and cultured with VERO-E6 cells. (paranormal.lt)
  • Scanning electron microscopy (SEM) depends on a focused electron beam scanned over the surface of a sample. (lu.se)
Immunofluorescence3
  • In the present study, we investigated the localization of PRKRA in the mammalian testis by immunofluorescence and immunoelectron microscopic techniques. (hindawi.com)
  • The capacity of mouse CABYR isoforms to associate into dimers and oligomers, and the relationships between CABYR and other FS proteins were studied by gel electrophoresis, Western blotting, immunofluorescence, immunoprecipitation and yeast two-hybrid analyses. (biomedcentral.com)
  • BACKGROUND: Accurate diagnosis of epidermolysis bullosa (EB) has significant implications for prognosis, management, and genetic counseling.OBJECTIVE: To describe diagnostic testing patterns and assess diagnostic concordance of transmission electron microscopy (TEM), immunofluorescence mapping (IFM), and genetic analysis for EB.METHODS: A retrospective cohort of patients enrolled in the Epidermolysis Bullosa Clinical Characterization and Outcomes Database from January 1, 2004 to July 8, 2019 was included. (stanfordchildrens.org)
Neurons1
Synapses3
  • By electron microscopy, most DβH terminals did not make synapses, but when they did, they formed both asymmetric and symmetric synapses. (frontiersin.org)
  • To understand better the cellular mechanisms of NE's contributions to fear learning, we examined the anatomical organization of NE terminals and βARs in the LA. In this study, we employed immunoelectron microscopy to determine whether terminals immunoreactive for dopamine beta-hydroxylase (DβH), the synthetic enzyme for NE, form synaptic junctions in the LA and if so, examine these synapses and identify the post-synaptic targets on NE terminals. (frontiersin.org)
  • 2006). Moreover, we carried out studies on the mechanism whereby β-amyloid antibodies can reduce Aβ peptides and protect synapses in cellular models of AD, providing a biological mechanism for a leading therapeutic direction for AD: Aβ immunotherapy (Tampellini et al. (lu.se)
Localization2
  • Previously, we investigated the localization of several nuage-resident proteins in the nuage structures of spermatogenic cells by immunoelectron microscopy (IEM) [ 22 - 26 ]. (hindawi.com)
  • The objective of this study was to identify the PDE10A isoforms expressed in the testis and germ cells, and to determine the presence and localization of PDE10A in mature spermatozoa. (plos.org)
Microscopic2
  • Using double-labelling immunoelectron microscopic techniques, co-clustering between GABA B1 and GIRK2 was detected in dendritic spines, whereas they were mainly segregated in the dendritic shafts. (springer.com)
  • From our study, we also suggest electron microscopic and biochemical studies for better diagnosis of these pathogens. (ajol.info)
Transmission2
  • Epidemiological studies including mapping, stratification and establishing cause and effect relationship using Geographic Information System (GIS) site specific dynamics of infection, transmission and disease and estimation of disease and economic burden. (icmr.nic.in)
  • Initiate molecular epidemiology studies to understand transmission dynamics. (icmr.nic.in)
Biochemical1
  • Study the biochemical and molecular characteristics of SAG responsive and unresponsive isolates. (icmr.nic.in)
Effectiveness1
  • Effectiveness of immuno-prophylactic at individual and community level. (icmr.nic.in)
Humans1
  • Information from studies of exposed humans and laboratory animals indicates that absorbed CDDs are distributed preferentially to fatty tissues and to a lesser extent, the liver (ATSDR 1998). (cdc.gov)
Mice1
  • To answer this question, the crypts that produce the dome epithelial cells were studied and the distribution of M cells over the domes was determined in mice. (nih.gov)
Secondary1
  • This makes it possible to study both the topography (secondary electrons) and detect contrast between areas with different atomic compositions (backscatter electrons), for example, the surface distribution of electron dense immuno-labels (nanogold or Quantum dots). (lu.se)
Substances1
  • Immuno-electron microscopy, also referred to as EM immunolabelling and immuno-EM, is a technique that tags antibody molecules with electron-dense substances, usually, and most effectively, being small gold particles, which are seen during the analysis as easy to spot dark dots. (news-medical.net)
Infections1
  • it may cause fatal infections in immuno- nomic next-generation sequencing (mNGS) of fresh compromised patients, but diagnosis is often delayed. (cdc.gov)
Cells5
  • Most studies of signaling pathways analyze the levels of whole populations of modified/unmodified proteins in a group of cells. (uclv.cu)
  • This study investigated the effect of low-intensity blue light on the albino Wistar rat retina, including intrinsically photosensitive retinal ganglion cells (ipRGCs). (mdpi.com)
  • We study whether PRKRA localizes to nuage of spermatogenic cells of rat and mouse. (hindawi.com)
  • Our study observed that the gold particles were clustered on the filamentous extension of the tip of the cells. (ajol.info)
  • Using immuno-gold electron microscopy of plasma membrane sheets coupled with spatial point analysis of validated microdomain markers, morphologically featureless microdomains were visualized in HeLa cells at high resolution. (tamu.edu)
Initiate3
  • Initiate basic, operational and preventive studies on HIV-TB. (icmr.nic.in)
  • Initiate studies on chemoprophylaxis of TB in HIV-TB patients. (icmr.nic.in)
  • 2011). Using dual-immuno-electron microscopy we further showed that early tau alterations initiate in Aβ accumulating synaptic terminals in the brain (Takahashi et al. (lu.se)
Demonstrate1
  • For this reason and because of the body of literature from hundreds of different Chlamydia studies that demonstrate a tremendous variability in diagnosing, identifying, and localizing Chlamydia in clinical specimens, we went to the extreme in our investigations by using a large variety of techniques on different clinical specimens. (alzforum.org)
Genetic3
  • Another area in which it has made a significant impact is that of genetic study, where it has been used to determine the role of specific gene products, elucidating their function in vital biological processes. (news-medical.net)
  • After other studies demonstrated high prevalence and have evolved and disseminated into the human population genetic diversity of SFVs in monkeys and apes in Ga- through various distinct mechanisms. (cdc.gov)
  • Studies on Molecular Epidemiology of Enteric Pathogens - Genetic characterization of V. cholerae strains. (icmr.nic.in)
Human2
  • Recent immunochemical studies have found that normal human sera (NHS) contain bactericidal anti-LOS antibodies that bind to the oligosaccharide (OS) moiety of neisserial LOS. (go.jp)
  • Inhalation and dermal exposure to CDDs are of lesser concern than oral exposure (because ingestion of CDDs in food is thought to be the principal route of exposure for the general population), but limited information from exposed human and animal studies indicate that CDDs can be absorbed by these routes. (cdc.gov)
Prevalence2
  • Microbiologic goals were to 1) determine, by using specifi c serologic studies of these high-risk populations are thus necessary to and molecular methods, the prevalence of SFV infection obtain new insights into the early events of this emergence in the adult population of different ethnic groups (includ- process ( 1 - 4 ). (cdc.gov)
  • Hypersensitivity pneumonitis was originally described in 1713 as an occupational lung disease in grain workers and later, in 1932, in farmers inhaling moldy hay contam- inated with thermophilic actinomyces, hence the term farmer's lung.1 With this recog- nition, modernization of farming methods has resulted in the reduction in farmer's lung prevalence estimated at 0.5% to 3% of exposed farmers in studies spanning from 1980 to 2003. (cdc.gov)
Light2
  • Correlative Light- and Electron Microscopy with chemical tags , Mario Perkovic a , Michael Kunz a , Ulrike Endesfelder b , Stefanie Bunse c , Christoph Wigge a , Thou Yu a , Victor-Valentin Hodirnau a , Margot P.Scheffer a . (leica-microsystems.com)
  • The use of electrons allows 10.000 x plus magnification, which is not possible using light microscopy and atomic resolution. (lu.se)
Antibodies1
  • Study of anti-cytokine antibodies in leprosy. (icmr.nic.in)
Analysis2
Results1
  • The results of the present study strongly suggest that neutrophil elastase may represent a common pathogenic link between emphysema and fibrosis. (biomedcentral.com)
Vacuum1
  • with vacuum compatible sealing of liquids in electron-transmitting thickness, K-kits are micro reaction chambers for countless experiments in materials, chemical, and biological research. (matek.com)
Molecular4
  • The techniques are commonplace in biology and molecular biology labs, and are used for a variety of applications in a wide range of fields of study, from oncology to hydrobiology. (news-medical.net)
  • Studies to understand the immunological and molecular basis of host parasite interaction. (icmr.nic.in)
  • Application of decision making tools, particularly mathematical models for forecasting the trend of infection/disease and predicting the long term impact of intervention and selection of appropriate site specific strategy and duration of intervention(s) (Statistical /Mathematical models, economic evaluations, rapid assessment procedures and mapping, sampling protocols, algorithms for morbidity management, molecular epidemiology, DNA fingerprinting for exposure studies). (icmr.nic.in)
  • Molecular epidemiological studies using RAPD, IS 6110, AFLP and other methods. (icmr.nic.in)
Brain3
  • Using immunogold labeling with compartmental markers and electron microscopy on neocortical biopsies from AD brain, we unequivocally identified autophagosomes and other prelysosomal autophagic vacuoles (AVs), which were morphologically and biochemically similar to AVs highly purified from mouse liver. (elsevierpure.com)
  • PDE10, which is more effective on cAMP than cGMP, has been known for almost 15 years and is mostly studied in the brain where it is associated with neurological disorders. (plos.org)
  • In the latest reports, electron microscopy, immuno-electron microscopy, and culturing from brain tissues were not performed. (alzforum.org)
Diseases3
  • This article summarizes the antiviral and Immuno boosting efficacy of the plant for exploiting its potential as a great (natural) antiviral agent in modern medicine for combating various virus diseases. (scitcentral.com)
  • Structural studies of cell transition in pathogenesis of mycobacterial diseases. (icmr.nic.in)
  • At the time we started this study, we really didn't know much about the duration of virus shedding,' says senior author Vincent Munster, a virologist at the National Institute of Allergy and Infectious Diseases. (paranormal.lt)
Western2
  • These days, immunohistochemical staining is just one of several established immunostaining techniques, including enzyme-linked immunosorbent assay, flow cytometry, immuno-electron microscopy, and Western blotting. (news-medical.net)
  • ELISA et transfert Western afin d'étudier la réaction croisée avec des sérums humains en provenance de zones endémiques pour la filariose de Bancroft. (who.int)
Process2
  • Three dimensional study of mycobacterial granuloma to understand disease process. (icmr.nic.in)
  • Electron microscopy is the process of greatly magnifying tiny particles in order to see and analyse them. (lu.se)
Samples1
  • Munster's lab at NIAID's Rocky Mountain Laboratories in Hamilton, Montana, began studying samples that were regularly collected from the patient's upper respiratory tract. (paranormal.lt)
Techniques1
  • We cannot stop short of intensifying these studies and we must work to develop standard techniques that can be applied by laboratories analyzing clinical specimens. (alzforum.org)
Dynamics1
  • Studies on dynamics of disease progression for disease management. (icmr.nic.in)
Community1
  • This article summarizes the rationale, aims, and overall design of the Childhood Obesity Declines Project (COBD), which was the first of its kind to systematically study and document the what, how, when, and where of community-based obesity strategies in four distinct communities across the nation. (cdc.gov)
Strain1
Present study1
  • The present study aims to review the antiviral potential of W. somnifera ingredients against COVID-19. (scitcentral.com)
Early2
  • The separation of emphysema from fibrosis is not as clear-cut as it was thought in early studies. (biomedcentral.com)
  • He was contacted in April by infectious disease specialist Francis Riedo, a study co-author, about a patient in Kirkland, Washington, who had been infected very early in the COVID-19 pandemic. (paranormal.lt)
Content1
  • Additionally, the lungs were also assessed for desmosine content and for the determination of elastase levels in the pulmonary interstitium by means of immunoelectron microscopy. (biomedcentral.com)
Animal2
  • In this study we investigate in experimental animal models of emphysema and fibrosis whether neutrophil elastase may constitute a pathogenic link between these two pathologies. (biomedcentral.com)
  • This study was done in two animal models in which emphysema and fibrosis were induced either by bleomycin (BLM) or by chronic exposure to cigarette-smoke. (biomedcentral.com)