Structure peptides membrane protein. Medical search. Frequent questions

All these features make peptoids highly attractive as synthetic analogues of lipids for self-assembly of membrane-mimetic 2D materials. (nature.com)
The pMMO is less well studied than the sMMO because of the instability of the pMMO upon removal of the polypeptides from the membrane lipids. (ncl.edu.tw)
They can be generated with energies and wavelengths appropriate for probing a range of biological structures, from small molecules such as lipids or peptides, to larger molecules and molecular assemblies including viruses. (ill.eu)
Work encompasses the design of peptides that have the potential to form stable pores in lipid membranes, the characterisation of peptide interactions with membrane lipids and the physical organic chemistry of peptide reactions in membranes. (dur.ac.uk)
Recent work has begun to establish the factors that control the innate reactivity of peptides towards acyl transfer from membrane lipids (intrinsic lipidation). (dur.ac.uk)
Previous studies suggest that membrane lipids may regulate proteolytic processing of the amyloid precursor protein (APP) to generate amyloid-beta peptide (Abeta). (edu.au)
The interplay between lipids and embedded proteins in plasma membrane is complex. (unifg.it)
Membrane proteins affect the stretching or disorder of lipid chains, transbilayer movement and lateral organization of lipids, thus influencing biological processes such as fusion or fission. (unifg.it)
Membrane lipids can regulate some protein functions by modulating their structure and organization. (unifg.it)
These may be different molecules within the cells like proteins, polysaccharides, or nucleoproteins and may also be the whole cell, like a tumor cell or organisms like bacteria, viruses, fungi, parasites, or agents containing genetic material such as nucleic acids or lipids. (medscape.com)

Based on our expertise in medium to high-throughput screening approaches involving microbes and a background in yeast cell biology, we expand the knowledge on membrane-related processes and extend the molecular toolboxes for applications on industrial scale. (tugraz.at)
Both experimental and molecular dynamics simulation results show that peptoids assemble into membranes through an anisotropic formation process. (nature.com)
Led by Michel Goedert and Sjors Scheres of the MRC Laboratory of Molecular Biology, Cambridge, England, U.K., the study describes an array of high-resolution structures of tau protofibrils plucked from postmortem brain samples. (alzforum.org)
These studies] present new evidence for a link between the molecular conformation of the tau protein and the resulting clinicopathologic phenotype," commented Lary Walker of Emory University, Atlanta. (alzforum.org)
Molecular dynamics simulations of membranes with embedded proteins and peptides: porin, alamethicin and influenza virus M2. (ox.ac.uk)
The Yale team described the crystal structure of a protease - a protein that acts like molecular scissors, cutting up proteins into component parts - found in membranes of an a single-celled organism. (yale.edu)
Molecular genetics of puroindolines and related genes: regulation of expression, membrane binding properties and applications. (usda.gov)
The researchers believe that these molecular interactions can explain why so many viruses make holes in their target membranes, why the fusion machinery of influenza variants typically does not mutate, and how spike proteins allow viral genetic material to enter the cell. (nih.gov)
The bulk of Isabella Karle's series is represented by her Research File , which contains a significant volume of raw crystallographic data, notes, figures and illustrations of molecular structures, and correspondence with scientific collaborators at a variety of national and international institutions. (loc.gov)
Specific HIV-1 proteins are fractionated according to molecular weight by electrophoresis on a polyacrylamide slab gel in the presence of sodium dodecyl sulfate (SDS). (cdc.gov)
If antibodies to any of the major HIV-1 antigens are present in the specimen in sufficient concentration, bands corresponding to the position of one or more of the following HIV-1 proteins (p) or glycoproteins (gp) will be seen on the nitrocellulose strip: p17, p24, p31, gp41, p51, p66, gp120, gp160 (number refers to apparent molecular mass in kilodaltons). (cdc.gov)

We are interested in processes that involve biological membranes. (tugraz.at)
Membrane proteins (which make up approximately one-third of the total number of proteins) are responsible for many important properties and functions of biological systems: they transport ions and molecules across the membrane, they act as receptors, and they have roles in the assembly, fusion, and structure of cells and viruses. (miamioh.edu)
Many studies suggest individual yet co-operative modes of action of the two PIN proteins in determining kernel texture, and significant evidence is accumulating that the proteins have in vivo and in vitro antimicrobial activities, shedding light on the biological roles of this unique ensemble of proteins. (usda.gov)
Dr John M Sanderson has research interests in Biophysical Chemistry, with a particular emphasis on biological membranes. (dur.ac.uk)
Research expertise in the group includes amino acid and peptide synthesis, liposome technology and techniques for studying biological membranes, including surface tensiometry, calorimetry, x-ray scattering, optical microscopy and mass spectrometry. (dur.ac.uk)
Post-translational modifications to aquaporin-0 are being examined by mass spectrometry and reveal a diverse lipidation profile at each of the two lipidation sites of the protein (with Prof. R. A. Quinlan, Biological Sciences, Durham University). (dur.ac.uk)
The membrane-binding properties of proteins from enveloped viruses are being characterised by a combination of microscopy, chemical biology and structral biology approaches (with Dr R. P. Yeo, Biological Sciences, Durham University). (dur.ac.uk)
and, regulation of biological processes such as chromosomal folding, protein secretion and intracellular signaling. (berkeley.edu)
Due to their large surface area and ability to interact with proteins and peptides, graphene oxides offer valuable physiochemical and biological features for biomedical applications and have been successfully employed for optimizing scaffold architectures for a wide range of organs, from the skin to cardiac tissue. (mdpi.com)
This project aims to study the mechanism of formation and structure of complex lipid bilayers, a lipid composition that mimics biological membranes (i.e. composed of several lipid types) on a variety of substrates including structured surfaces consisting of nanowire forests. (lu.se)

Current work is directed towards the study of protein-membrane interactions over all timescales and the preparation of asymmetric lipid membranes for biophysical studies of membrane activity (funded by an EPSRC programme grant). (dur.ac.uk)
Current work is examining the chemistry exhibited by drug molecules after their adsorbtion to lipid membranes. (dur.ac.uk)
In this work, we investigated the role of cholesterol in hCt incorporation and channel formation in planar lipid membranes made up of palmitoyl-oleoyl-phosphatidylcholine in which no channel activity had been found. (unifg.it)
The results obtained in this study indicate that cholesterol promotes hCt incorporation and channel formation in planar lipid membranes, suggesting a possible role of sterol as a lipid target for hCt. (unifg.it)

Major histocompatibility complex class I (MHC I) molecules present antigenic peptides to cytotoxic T cells to eliminate infected or cancerous cells. (researchgate.net)
For example, self-assembly of lipid molecules and membrane proteins is crucial in defining cell architecture and enabling cell function 2 . (nature.com)
One of their most important advantages is that they interact quite strongly with hydrogen nuclei, so can pick out all the hydrogen atoms in a structure, including those in water molecules. (ill.eu)
Molecules can have complicated electronic structure, where the mean-field assumption does not hold, and so-called multiconfiguration based methods are necessary. (lu.se)

From left to right: Step one, fusion peptides bind to the inner surface of the vacuole membrane. (nih.gov)
Step two, the fusion peptides bind to each other, tilting and pinching the inner and outer surface of the membrane together, forming a hole. (nih.gov)
The researchers found that within the acidic environment of the vacuole, fusion peptides on the spike protein bind to the vacuole's inner membrane. (nih.gov)
Fusion peptides bind to the inner lipid layer, and adjacent fusion peptides bind to each other. (nih.gov)
This attraction of the fusion peptides pulls membrane proteins together and pushes away the fats, thinning the membrane at the binding site. (nih.gov)

The transporter associated with antigen processing (TAP) shuttles proteasomally generated peptides into the ER for MHC I loading. (researchgate.net)
This subfamily includes lantibiotic ABC transporter permease subunits EpiE, MutE, SlvE and NisE, which are highly hydrophobic, integral membrane proteins, and part of the bacitracin ABC transport system that confers resistance to the Gram-positive bacteria in which this system operates, specifically to the lantibiotics mutacin, epidermin, nisin and salivaricin, respectively. (nih.gov)
In salivaricin D-producing strain Streptococcus salivarius 5M6c, self-immunity against the intrinsically trypsin-resistant salivaricin is mediated via ABC transporter proteins SlvF, SlvE and SlvG, while in Lactococcus lactis, self-immunity against nisin is mediated by the ABC transporter NisFEG. (nih.gov)
Crystal structure of the oligopeptide transporter PepTSt in the inward open conformation at 2.0 Å resolution. (embl-hamburg.de)
Here the transporter adopts different conformational states, allowing the substrate binding site to face either side of the membrane. (embl-hamburg.de)
The TAP1 and TAP2 proteins attach (bind) together to form a protein complex called transporter associated with antigen processing (TAP) complex. (medlineplus.gov)
Nickel ABC transporter, substrate-binding protein NikA / Solute-binding protein family 5, conserved site / Bacterial extracellular solute-binding proteins, family 5 signature. (pdbj.org)
Involved in the association of MHC class I with transporter associated with antigen processing (TAP) and in the assembly of MHC class I with peptide (peptide loading). (lu.se)

For example, the structure of a target peptide aiming for the mitochondrial environment differs in terms of length and shows an alternating pattern of small positively charged and hydrophobic stretches. (wikipedia.org)
Apoptotic stimuli activate and oligomerize the proapoptotic proteins Bak and Bax, resulting in mitochondrial outer-membrane permeabilization and subsequent cell death. (rcsb.org)
For the proteins associated with cystic fibrosis, mitochondrial disease, cancer and a wide variety of metabolic disorders, physical, biochemical and catalytic studies are carried out to derive a picture of both normal and abnormal structure and function. (utoronto.ca)
Fatty liver is associated with reduced SIRT3 activity and mitochondrial protein hyperacetylation. (tamu.edu)

Our particular focus in the yeast lipid field are sterols, their biosynthesis and homeostasis, and their interactions with membrane proteins. (tugraz.at)

An ability to develop sequence-defined synthetic polymers that both mimic lipid amphiphilicity for self-assembly of highly stable membrane-mimetic 2D nanomaterials and exhibit protein-like functionality would revolutionize the development of biomimetic membranes. (nature.com)
We insert synthetic peptides into proteins of interest via tandem protein trans-splicing using two orthogonal split intein pairs and validate our approach by investigating different aspects of GFP, Na V 1.5 and P2X2 receptor function. (biorxiv.org)
Because the approach can introduce virtually any chemical modification into both intracellular and extracellular regions of target proteins, we anticipate that it will overcome some of the drawbacks of other semi-synthetic or ribosome-dependent methods to engineer proteins. (biorxiv.org)
Semi-synthetic approaches offer an alternative means to manipulate proteins post-translationally, but these modifications have typically been performed in vitro 3 - 8 . (biorxiv.org)
We thus sought to complement these approaches with a method that could incorporate synthetic peptides carrying multiple post-translational modifications (PTMs) or ncAAs into both cytosolic and membrane proteins in live eukaryotic cells. (biorxiv.org)
Through the use of model membranes and synthetic peptides we also have a clearer understanding of the significance of the cysteine backbone and tryptophan-rich domain in the PIN proteins and their helicoid tertiary structures in relation to their membrane-active properties. (usda.gov)
All specimens were tested using the Synthetic Peptide Enzyme Immunoassay (EIA) (Genetic Systems HIV-1/HIV-2 Peptide EIA) for the detection of antibody to human immunodeficiency virus type 1 or type 2 (HIV-1 or HIV-2) or both (Bio-Rad Laboratories, Redmond, WA). (cdc.gov)

Deepened knowledge on the biophysical chemistry of proteins with emphasis on properties rather than methods. (lu.se)
This journal presents the latest developments in the area of protein chemistry. (eurekaselect.com)

We engineer the membranes of microbes, i.e. bacteria and yeasts, regarding lipid and membrane protein composition, either to understand the complex interactions of these hydrophobic compounds or to apply the modified microorganisms for interesting goals of industrial biotechnology. (tugraz.at)

To probe the two-stage model, the structures of peptides representing individual transmembrane helices of the disulfide bond forming protein B have been studied in trifluoroethanol solution as well as in detergent micelles using nuclear magnetic resonance (NMR) and circular dichroism spectroscopy. (rcsb.org)
These observations may indicate that in the native protein, tertiary interactions additionally stabilize the secondary structure of the individual transmembrane helices. (rcsb.org)
Although most type I membrane-bound proteins have signal peptides, the majority of type II and multi-spanning membrane-bound proteins are targeted to the secretory pathway by their first transmembrane domain, which biochemically resembles a signal sequence except that it is not cleaved. (wikipedia.org)
While secreted proteins are threaded through the channel, transmembrane domains may diffuse across a lateral gate in the translocon to partition into the surrounding membrane. (wikipedia.org)
This cleavage site is absent from transmembrane-domains that serve as signal peptides, which are sometimes referred to as signal anchor sequences. (wikipedia.org)
The tat system recognizes proteins with an elongated signal sequence containing a conserved R-R in a motif approximated by RRxFLK N-terminal to the transmembrane helix. (nih.gov)
The crystal structure of the protease called Flak from the archeon Methanococcus maripaludis is characterized by six transmembrane helices, the researchers found. (yale.edu)
Human IL-17 RB cDNA encodes a 502 amino acid (aa) residue type I membrane protein with a putative 17 aa signal peptide, a 275 aa extracellular domain, a 21 aa transmembrane domain and a 189 aa cytoplasmic tail. (rndsystems.com)

Dr. DeGrado's work focuses on the design of small molecule drugs, peptides, proteins and peptide mimetics. (pliantrx.com)

Signal peptides are extremely heterogeneous, many prokaryotic and eukaryotic ones are functionally interchangeable within or between species and all determine protein secretion efficiency. (wikipedia.org)
Pichia pastoris , a methylotrophic yeast, is our favourite host, which we employ for expression and secretion of recombinant soluble proteins as well as for the biosynthesis of valuable metabolites, e.g. plant terpenoids, through heterologous expression of membrane proteins. (tugraz.at)
We have uncovered several endogenous yeast proteins and mechanisms that render protein secretion or membrane protein expression more efficient and sustainable. (tugraz.at)
We have devised a novel, medium-throughput, random mutagenesis/screening protocol for improved protein secretion in P. pastoris. (tugraz.at)
We have applied multiple approaches to identify key genes that determine protein secretion rates in P. pastoris. (tugraz.at)
Antibodies can be found on the surface of lymphocytes as an integral part of the cell membrane protein or can be freely circulating in the blood or be part of one of the body's gland secretion. (medscape.com)

Tail-specific protease (Tsp) is a periplasmic enzyme that selectively degrades proteins bearing a nonpolar C-terminus. (embl.de)
Partial proteolysis by V8 protease at a single peptide bond immediately N-terminal to the PDZ domain resulted in two distinct and relatively stable fragments and complete loss of catalytic activity. (embl.de)
This membrane protein belongs to the GxGD membrane protease family. (yale.edu)
Here, we develop a calcium-triggered DNA-mediated membrane fusion strategy where fusion is regulated using surface-bound PEG chains that are cleavable by the calcium-activated protease calpain-1. (rsc.org)
A cysteine-rich metal-binding domain from rubella virus non-structural protein is essential for viral protease activity and virus replication. (tamu.edu)

This will allow membranes with tailored properties to be developed for applications in key membrane processes, such as antimicrobial peptide activity, protein interactions, and DNA transfection. (lu.se)

The most "famous" member of the family is a human protein called presenilin, which has been implicated in the production of amyloid beta, peptides that cause Alzheimer's disease. (yale.edu)
All recognized mutations for AD are associated with increased deposition of amyloid-beta (Abeta), a peptide fragment comprising 39-43 amino acids that derive from the catabolism of the amyloid precursor protein (APP) molecule. (medscape.com)

We are studying the mechanism of viral membrane fusion and its inhibition by drugs and antibodies. (stanford.edu)
Planar aggregation of the influenza viral fusion peptide alters membrane structure and hydration, promoting poration. (nih.gov)
Detailed site-specific glycan analysis of a wide variety of SARS-CoV-2 variants provides useful information toward the understanding of the role of protein glycosylation on viral protein structure and function and development of effective vaccines and therapeutics. (cdc.gov)

These proteins include those that reside either inside certain organelles (the endoplasmic reticulum, Golgi or endosomes), secreted from the cell, or inserted into most cellular membranes. (wikipedia.org)
A homologous system exists in eukaryotes, where the signal peptide directs the newly synthesized protein to the Sec61 channel, which shares structural and sequence homology with SecYEG, but is present in the endoplasmic reticulum. (wikipedia.org)
moves (transports) protein fragments (peptides) from foreign invaders into the endoplasmic reticulum. (medlineplus.gov)
Mutations in the TAP1 or TAP2 gene prevent formation of the TAP complex, which impairs the transport of peptides into the endoplasmic reticulum. (medlineplus.gov)

It is possible to determine the amino acid sequence of the N-terminal signal peptide by Edman degradation, a cyclic procedure that cleaves off the amino acids one at a time. (wikipedia.org)
An epitope may represent a linear amino acid sequence or antigenic tertiary structure. (medscape.com)

However, agonist-dependent activation of cell surface receptors is sometimes required to promote interaction with a PDZ protein. (embl.de)
The human IL-17 RB protein sequence is only 19.2% identical to the human IL-17 R sequence, but the two receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. (rndsystems.com)

Upregulation of several target genes has improved recombinant protein titers and yields. (tugraz.at)
Each of these genes provides instructions for making a protein that plays a role in helping the immune system recognize and fight infections. (medlineplus.gov)
The findings may inform the development of new technologies to combat infectious microbes and to insert medications, genes, and proteins into cells to treat diseases. (nih.gov)
The results revealed the mitogenome (GenBank: OQ181231) to occur as a circular DNA molecule of 16,376 bp with 51.001% AT content, including 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and 1 control region. (bvsalud.org)
The 22 tRNA genes demonstrated the ability to form a cloverleaf structure, with the exception of trnS1-NCU, which lacked the DHU arm present in other Erebidae mitogenomes. (bvsalud.org)

However, the limited set of chromatophore-encoded solute transporters appears insufficient for supporting metabolic connectivity or protein import. (frontiersin.org)
Here we show by mass spectrometric analyses of enriched insoluble protein fractions that, unexpectedly, nucleus-encoded transporters are not inserted into the chromatophore inner envelope membrane. (frontiersin.org)
Thus, despite the apparent maintenance of its barrier function, canonical metabolite transporters are missing in this membrane. (frontiersin.org)
Structural and functional analyses of MFS transporters suggest an alternating-access mechanism for the transport of substrates across the membrane. (embl-hamburg.de)
Proton-coupled oligopeptide transporters of the PepT family (also known as the POT family) are responsible for the uptake of a range of different di- and tripeptides, derived from the digestion of dietary proteins, and are highly conserved in all kingdoms of life. (embl-hamburg.de)
The best studied members of this family include the two human peptide transporters, PepT1 and PepT2. (embl-hamburg.de)
Insights into structure and function of transport regulators of nutrient transporters by structural systems biology approaches. (embl-hamburg.de)

Lantibiotics are small peptides, produced by Gram-positive bacteria, which are ribosomally-synthesized as pre-peptides and act by disrupting membrane integrity. (nih.gov)
A lack of these proteins impairs the body's immune response to bacteria, leading to recurrent bacterial infections. (medlineplus.gov)

Sahu ID, Craig AF, Dunagum MM, McCarrick RM, Lorigan GA. (2017) "Characterization of Bifunctional Spin Labels for Investigating the Structural and Dynamic Properties of Membrane Proteins Using EPR Spectroscopy" J Phys Chem B 121:9185-9195. (miamioh.edu)
The research in the Lorigan laboratory is focused upon the utilization of magnetic resonance spectroscopy to investigate the structural and dynamic properties of membrane-bound proteins and peptides and their interactions within the lipid bilayer. (miamioh.edu)

Sahu ID, Zhang R, Dunagan MM, Craig AF, Lorigan GA (2017) "Characterization of KCNE1 inside Lipodisq Nanoparticles for EPR Spectroscopic Studies of Membrane Proteins" J Phys Chem B 121:5312-5321. (miamioh.edu)

A signal peptide (sometimes referred to as signal sequence, targeting signal, localization signal, localization sequence, transit peptide, leader sequence or leader peptide) is a short peptide (usually 16-30 amino acids long) present at the N-terminus (or occasionally nonclassically at the C-terminus or internally) of most newly synthesized proteins that are destined toward the secretory pathway. (wikipedia.org)
Signal peptides function to prompt a cell to translocate the protein, usually to the cellular membrane. (wikipedia.org)
In prokaryotes, signal peptides direct the newly synthesized protein to the SecYEG protein-conducting channel, which is present in the plasma membrane. (wikipedia.org)
In addition, many signal peptides begin with a short positively charged stretch of amino acids, which may help to enforce proper topology of the polypeptide during translocation by what is known as the positive-inside rule. (wikipedia.org)
At the end of the signal peptide there is typically a stretch of amino acids that is recognized and cleaved by signal peptidase and therefore named cleavage site. (wikipedia.org)
Signal peptidase may cleave either during or after completion of translocation to generate a free signal peptide and a mature protein. (wikipedia.org)
The free signal peptides are then digested by specific proteases. (wikipedia.org)
Moreover, different target locations are aimed by different types of signal peptides. (wikipedia.org)
Nucleus aiming signal peptides can be found at both the N-terminus and the C-terminus of a protein and are in the majority of the cases retained in the mature protein. (wikipedia.org)
The co-translational pathway is initiated when the signal peptide emerges from the ribosome and is recognized by the signal-recognition particle (SRP). (wikipedia.org)
SRP then halts further translation (translational arrest only occurs in Eukaryotes) and directs the signal sequence-ribosome-mRNA complex to the SRP receptor, which is present on the surface of either the plasma membrane (in prokaryotes) or the ER (in eukaryotes). (wikipedia.org)
Once membrane-targeting is completed, the signal sequence is inserted into the translocon. (wikipedia.org)
In prokaryotes, the signal sequence of post-translational substrates is recognized by the SecB chaperone protein that transfers the protein to the SecA ATPase, which in turn pumps the protein through the translocon. (wikipedia.org)
In vertebrates, the region of the mRNA that codes for the signal peptide (i.e. the signal sequence coding region, or SSCR) can function as an RNA element with specific activities. (wikipedia.org)
Together with Syntaxin-1A and SNAP25, it forms the core membrane fusion machinery that is responsible for neurotransmitter release and, therefore, signal transmission between neurons. (researchgate.net)

We describe a statistical measure, Mass Distance Fingerprint, for automatic de novo detection of predominant peptide mass distances, i.e., putative protein mod- ifications. (lu.se)

The success of proteomics experiments, such as studies of protein function and cell signaling pathways, ultimately de- pends on how well the protein content in samples is identified and annotated. (lu.se)
The need to keep track of protein modifications is readily recognized by the proteomics community, and few repositories of known peptide modifications have been created. (lu.se)

Two critical factors during this dauntingly complex process appear to be the establishment of metabolic connectivity between the symbiotic partners, and the evolution of nuclear control over protein expression levels within the organelle. (frontiersin.org)
The SNARE complex assembles from vesicular Synaptobrevin-2 as well as Syntaxin-1 and SNAP25 both anchored to the presynaptic membrane. (researchgate.net)
As central part of the peptide-loading complex (PLC), TAP is targeted b. (researchgate.net)
Solution structure of the core SMN-Gemin2 complex. (tamu.edu)
Inside the virus, its ribonucleoproteins (RNPs) were shown, with their coiled structures, and three-bulbed polymerase complex on the ends. (cdc.gov)

The two-stage model for membrane protein folding postulates that individual helices form first and are subsequently packed against each other. (rcsb.org)
The extent of α-helical secondary structure coincided well, lending support for the two-stage model for membrane protein folding. (rcsb.org)
The isolated PDZ domain (amino acids 206-334) is capable of folding into a well-behaved structure and binds to a nonpolar peptide with a dissociation constant (K(D)) of 1.9 microM, similar to that of the intact Tsp protein. (embl.de)
In addition, reviews of experimental studies of protein folding and design are given special emphasis. (eurekaselect.com)

In this work, we show that substrate recognition of Tsp is mediated by a PDZ domain, a small protein module that promotes protein-protein interactions by binding to internal or C-terminal sequences of their partner proteins. (embl.de)
The method's focus is to globally detect mass differences, not to assign peptide sequences or modifications to individual spectra. (lu.se)
The goal is to assign acquired spectra to known peptide sequences and potential co- and post-translational modifica- tions. (lu.se)
The matching is com- plicated by the fact that there are protein modifications and the sequence databases store the unmodified sequences. (lu.se)

Dixit G, Dabney-Smith C, and Lorigan GA (2020) "The membrane protein KCNQ1 potassium ion channel: Functional diversity and current structural insights" BBA-Biomembranes, 1862, 5, 183148-183187. (miamioh.edu)
29, 2020 Researchers previously developed a filter membrane made out of whey proteins and activated carbon. (sciencedaily.com)

Future studies are targeted at finding techniques and methodologies to correct the abnormal cellular function derived from the behaviour of the mutant protein. (utoronto.ca)
Manipulation of proteins by chemical modification is a powerful way to decipher their function or harness that function for therapeutic purposes. (biorxiv.org)
Chemical or genetic engineering of proteins provides great potential to study protein function and pharmacology or to generate proteins with novel properties. (biorxiv.org)
In particular, the TAP1 and TAP2 proteins aid the function of MHC class I proteins . (medlineplus.gov)
Structure and relation to Function. (lu.se)
This is because the protein sequence/structure/function is encoded in DNA, and therefore the physicochemical properties of the nanoparticle are also encoded in DNA. (rsc.org)
In particular, we study the structure, function and mechanisms of metalloproteins and the binding of drug candidates to biomacromolecules. (lu.se)
While abnormal keratin filament structure and function can explain hypohidrosis and epithelial differentiation abnormalities, the absence of dermatoglyphics is not well understood. (medscape.com)
N-Glycosylation plays an important role in the structure and function of membrane and secreted proteins. (cdc.gov)
The relative abundance of sialylated glycans in the S1 subunit compared to the full-length protein could indicate differences in the global structure and function of these two species. (cdc.gov)

In TFE solution, peptides showed well-defined α-helical structures. (rcsb.org)
In micellar solution, the peptides also adopted a helical structure, albeit of reduced definition. (rcsb.org)
Furthermore, our mass spectrometric analysis revealed an expanded family of chromatophore-targeted helical repeat proteins. (frontiersin.org)
In 2017, they dazzled the AD field when they described the back-to-back C-shaped structures that stacked in paired helical and straight filaments of that disease ( Jul 2017 news ). (alzforum.org)
Solution Structure, Dimerization, and Dynamics of a Lipophilic $\alpha$/310-Helical, C$\alpha$-Methylated Peptide. (oregonstate.edu)

Integral membrane proteins are a challenging class of proteins in terms of their structural and functional characterisation. (embl-hamburg.de)
Furthermore, we are developing new ways to stabilise integral membrane proteins in vitro upon extraction from their natural environment. (embl-hamburg.de)
I have research funding from the NIH and NSF to study the structural and dynamic properties of integral membrane proteins. (miamioh.edu)

Furthermore, chromatophore-localized biosynthetic pathways as well as multiprotein complexes include proteins of dual genetic origin, suggesting that mechanisms evolved that coordinate gene expression levels between chromatophore and nucleus. (frontiersin.org)
Yale School of Medicine researchers have described for the first time the structure of a member in an enigmatic family of proteins linked to Alzheimer's disease, a crucial early step in understanding key mechanisms of the disease and designing drugs to combat dementia. (yale.edu)
While several non-native membrane fusion mechanisms have been demonstrated, few can respond to external stimuli. (rsc.org)

One of the few antigens against which mammals develop protective immunity is the highly polymorphic OspC protein, encoded by the ospC gene on the cp26 plasmid. (cdc.gov)
By means of DNA sequencing of a large sample collection of the pathogen from across the United States, we studied the gene for the bacterium's highly diverse OspC protein, protective immunity against which develops in animals. (cdc.gov)

Here we present a new approach to incorporate single or multiple post-translational modifications or non-canonical amino acids into soluble and membrane proteins expressed in eukaryotic cells. (biorxiv.org)

Cell membranes compartmentalise metabolic processes and serve as selective barriers for permeation. (embl-hamburg.de)
The oligomerization and fibrillation processes of hCt can be modulated by different factors such as pH, solvent, peptide concentration, and chaperones. (unifg.it)

An influenza virus' RNP is composed of both RNA and protein. (cdc.gov)

New technologies for purifying membrane-bound protein complexes in combination with cryo-electron microscopy (EM) have recently allowed the exploration of such complexes under near-native conditions. (researchgate.net)

It imports hundreds of nucleus-encoded proteins, and diverse metabolites are continuously exchanged across the two chromatophore envelope membranes. (frontiersin.org)

We further demonstrated the use of peptoid membranes as a robust platform to incorporate and pattern functional objects through large side-chain diversity and/or co-crystallization approaches. (nature.com)
The behaviour of membrane proteins in membranes is being examined using a range of analytical approaches. (dur.ac.uk)
The method can be used for automated detection of chemical/post- translational modifications, quality control of experiments and labelling approaches, and to control the modification settings of protein identification tools. (lu.se)
These approaches promise to enable researchers to assess, on a large scale, both expression level and functional state of the proteins that carry out most functions in a cell. (lu.se)

Here we identify specific BH3 peptides capable of "unlatching" Bak and describe structural insights into Bak activation and oligomerization. (rcsb.org)
Structural and dynamic insights into the binding mode of POTs to peptides, drugs, and inhibitors. (embl-hamburg.de)

Ribosomes are then physically docked onto the cytoplasmic face of the translocon and protein synthesis resumes. (wikipedia.org)
The post-translational pathway is initiated after protein synthesis is completed. (wikipedia.org)
When a defined protein/peptide (or combinations thereof) control and define the synthesis of an inorganic nanoparticle, the result is a cloneable NanoParticle (cNP). (rsc.org)
Facilities include those for protein-sequence analysis, peptide and oligonucleotide synthesis, access to the synchrotron at Lawrence Berkeley National Lab for X-ray crystallography, and NMR spectrometry. (berkeley.edu)
Measuring muscle protein synthesis in humans and the influence of nutritional state. (tamu.edu)

The oxysterol-binding protein superfamily: new concepts and old proteins. (tamu.edu)

To infect a cell, spike proteins-projections on the virus's outer surface-bind to the cell's surface. (nih.gov)
In this report, we conducted mass spectrometric analysis of the N-glycosylation profiles of recombinant spike proteins derived from four common SARS-CoV-2 variants classified as Variant of Concern, including Alpha, Beta, Gamma, and Delta along with D614G variant spike as a control. (cdc.gov)
We conducted a comprehensive mass spectrometric analysis of the N-glycosylation profiles of the SARS-CoV-2 spike proteins using signature ions-triggered electron-transfer/higher-energy collision dissociation (EThcD) mass spectrometry. (cdc.gov)

Modulation of effector protein activity by proteases provides a critical layer of posttranslational control that enables rapid enzymatic regulation of target proteins. (nih.gov)

Dendrogram of a proposed structure-based classification of tauopathies. (alzforum.org)

Two distinct forms of MMO are known to exist at different cellular locations, a cytoplasmic (soluble) MMO and a membrane-bound (particulate) MMO. (ncl.edu.tw)

Because there are no peptides for MHC class I proteins to bind, they are broken down, which results in a shortage of MHC class I proteins on the surface of cells. (medlineplus.gov)

Consequently, a lot of effort is put into identifying the constituent proteins using mass spectrometric methods. (lu.se)

It mediates fusion of synaptic vesicles with the presynaptic plasma membrane resulting in exocytosis of neurotransmitters. (researchgate.net)
PDZ domain proteins are frequently associated with the plasma membrane, a compartment where high concentrations of phosphatidylinositol 4,5-bisphosphate (PIP2) are found. (embl.de)
Therefore, nutrient transport through the plasma membrane is essential to maintain homeostasis within the cell. (embl-hamburg.de)

Photoaffinity labeling with a fluorescent nonpolar peptide caused the covalent attachment of the peptide to a single site on the Tsp protein. (embl.de)

We also develop software for coarse grained protein simulations. (lu.se)

Herein we report the first example of self-assembling highly stable and self-repairing membrane-mimetic 2D nanomaterials by designing lipid-like sequence-defined peptoids. (nature.com)
The proposed method is novel because it works independently of protein sequence databases and without any prior knowledge about modifica- tions. (lu.se)
PTMs from this list in conjunction with a protein sequence and a few precursor masses. (lu.se)
The SARS-CoV-2 spike protein (S) is a prime example, having 22 potential sites of N-glycosylation per protein protomer, as predicted from the primary sequence. (cdc.gov)

The protein then switches to an active form when DNA damage is detected to trigger the process of cell self-destruction. (elifesciences.org)
Indeed, most live cell applications of PTS utilize single split inteins for the purpose of N/C-terminal tagging 16 - 18 or manipulating protein assembly/expression 19 , 20 . (biorxiv.org)
Researchers at the National Institutes of Health believe they have discovered how influenza viruses open a hole in the cell membrane to inject genetic material into the cell. (nih.gov)
The cell's outer membrane envelops the virus in a pocket-like structure called a vacuole, which encapsulates the virus and takes it inside the cell. (nih.gov)
They target specific surface structures and either eliminate the virus from the host bloodstream or destroy the infected cell in which it resides. (forbes.com)
Cholesterol is a lipid of cell membranes that has been intensively investigated and found to be associated with some membrane proteins and to play an important role in diseases. (unifg.it)

Peptide structures in TFE were compared to the structures of full-length protein obtained by X-ray crystallography and NMR. (rcsb.org)
However, the conformation of some amino acid side chains differs between the structures of peptide and full-length protein. (rcsb.org)

Anatomy39

Organisms13

Diseases1

Chemicals and Drugs107

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes62

Disciplines and Occupations2

Technology, Industry, Agriculture7

Information Science3

Health Care1

Lipids10

Molecular11

Biological10

Lipid membranes4

Molecules4

Fusion peptides5

Transporter8

Mitochondrial4

Interactions with membrane1

Synthetic7

Chemistry2

Hydrophobic1

Transmembrane8

Mimetics1

Secretion6

Protease5

Antimicrobial1

Amyloid beta2

Viral3

Endoplasmic reticulum4

Amino acid seq2

Receptors2

Genes5

Transporters7

Bacteria2

Spectroscopy2

Nanoparticles1

Signal15

Putative1

Proteomics2

Complex5

Folding4

Sequences4

20202

Function10

Helical5

Integral3

Mechanisms3

Immunity2

Eukaryotic1

Processes2

Influenza virus1

Complexes1

Metabolites1

Approaches4

Insights2

Synthesis5

Superfamily1

Spike proteins3

Target proteins1

Classification1

Soluble1

Bind1

Mass spectrometric1

Plasma3

Covalent1

Simulations1

Sequence4

Cell6

Full-length protein2