Structure peptide library. Medical search. Frequent questions

The claimed biochip consists of a three-dimensional mesoporous layer, where the mesoporous layer is chemically modified to recognize labeling DNAs, proteins, peptides, saccharides, and cells. (genomeweb.com)
The patent provides the means for producing libraries of peptide structures for drug screening applications that are capable of folding or assuming their native conformations independently of artificial scaffolds or flanking sequences in the proteins from which they are derived. (genomeweb.com)
Peptides are short chains of amino acids that can bind to proteins and change their function. (epfl.ch)
First, binding affinity: Good binding requires difficult peptide architectures and amino acid sequences that are perfectly complementary in shape and charge to the surface of their target proteins. (epfl.ch)
Modeling of the chemical properties of proteins is a challenging task in modern computational biochemistry, leading researchers to focus on the properties of small, representative, protein-like structures, such as peptides. (peerj.com)
Predicting evolutionary site variability from structure in viral proteins: buriedness, flexibility, and design. (openwetware.org)
However, as actual yields vary widely for individual proteins, GlycoFi is focusing on the improvement of protein yields in P. pastoris as part of the company's long term mission to develop yeast-based protein production technology for manufacturing therapeutic proteins with human glycosylation structures. (sbir.gov)
The Pentelute Lab aims to invent new chemistry for the efficient and selective modification of proteins, to 'hijack' these biological machines for efficient drug delivery into cells and to create new machines to rapidly and efficiently manufacture peptides and proteins. (pentelutelabmit.com)
Biosynthesis and intracellular trafficking of secretory and lysosomal/vacuolar proteins, glycosylation and other post-translational protein modifications as determinants of protein structure and function, catalytic properties and physiological functions of proteases and glycosylation enzymes, proteolysis and its pathological and biotechnological relevance, recombinant expression of biotherapeutics in plants, plant membrane protein biochemistry. (boku.ac.at)
Collagen peptides are made by breaking down whole collagen proteins into smaller pieces. (medlineplus.gov)

Chapter 2 discusses the synthesis of a library of N-terminal modified peptides created using a one-bead one-compound (OBOC) approach. (uwo.ca)
Chapter 4 discusses the synthesis of an OBOC library with an imaging moiety integrated within the peptide structures. (uwo.ca)
A two-pool OBOC strategy was developed to distribute the fluorine-containing amino acid throughout the peptide sequence during library synthesis. (uwo.ca)
Our custom peptide synthesis service offers high-quality, reliable and cost-effective products to support your research. (2bscientific.com)
We have unrivalled expertise in the synthesis of complex, constrained and other special peptides and our scientists are happy to assist in selecting the most appropriate peptide specification for your application. (2bscientific.com)
A typical custom synthesis peptide run will yield between 5 - 50 mg of the peptide in question, depending upon the number of amino acids and complexity of the product. (2bscientific.com)
Turnaround time for a peptide synthesis is typically 1-3 weeks but may be longer for peptides over 60 amino acids, those with difficult modifications or if TFA removal, or non-routine analysis is required. (2bscientific.com)
Our custom peptide synthesis partner is able to synthesize any kinds of peptides from 2 to 100+ amino acids with a 99% purity rate. (2bscientific.com)
They also have state of the art Peptide Library Synthesis for hundreds of peptides. (2bscientific.com)
We can offer one-stop protein structural biology services from gene synthesis to cryo-EM structure. (ks-vpeptide.com)
NovoPro has a rapid high-throughput parallel peptide synthesis platform that enables our customers to synthesize larger quantities of high purity peptides at lower cost than traditional methods. (novoprolabs.com)
With ProteoGenix's cost-effective method of peptide library synthesis , you can now employ high-quality peptides for a fraction of the cost. (proteogenix.science)
Why choose ProteoGenix for overlapping peptide library synthesis? (proteogenix.science)

When experiment-specific spectrum library generation is either impossible or impractical, as is frequently the case with non-model organisms, sequence variants, splice isoforms, or scarce sample quantities, software tools such as Pecan 10 and DIA-Umpire 11 can detect peptides from DIA experiments without a spectrum library by directly searching every peptide in FASTA databases. (nature.com)
The precise sequence of amino acids that makes up the channel-blocking ball in potassium channels was identified through the creation a synthetic peptide. (wikipedia.org)
The peptide was built based on the sequence of a 20 amino acid residue from the Drosophila melanogaster's Shaker ShB protein and applied on the intracellular side of a non-inactivating channel in Xenopus oocytes. (wikipedia.org)
The main outcomes were development of dynamic peptide libraries for discovery of self-assembling peptides Nature Nanotechnology, 2016, discovery of customizable polymeric peptide pigments with sequence tunable properties Science, 2017 and the demonstration of amino acid encoded transient assemblies, showing transient electroconductance Nature Chemistry, 2018. (dtic.mil)
By using experimental techniques such as circular dichroism, small angle X-ray scattering, quartz crystal microbalance with dissipation monitoring, and neutron reflectometry, it was determined that changing the number of histidines in the peptide sequence did not affect the structure of the peptide dissolved in solution. (lu.se)
Because the four cysteines can be placed in many different ways along the sequence of amino acids, the double-bridging strategy allowed the researchers to generate an enormously large number of structurally diverse peptide architectures. (epfl.ch)
The scientists also found that the four cysteines could be bridged by two linkers in three different ways, giving rise to three different architectures from each single peptide sequence. (epfl.ch)
Integrating sequence variation and protein structure to identify sites under selection. (openwetware.org)
Reverse phase HPLC and an electroblot transfer technique to a membrane were used to purify the peptide for amino acid composition and sequence analysis. (usda.gov)
We report the successful use of CFB for the formation of an array of sequence-diverse lasso peptides that include known examples as well as a new predicted lasso peptide from Thermobifida halotolerans. (illinois.edu)
By evaluating more than 1000 randomly chosen variants, we show that the lasso-forming cyclase from the fusilassin pathway is capable of producing millions of sequence-diverse lasso peptides via CFB. (illinois.edu)
DNA sequence alignment analysis and the forecast-analysis of protein structure indicated that these seven antimicrobial peptide genes were not homologous to any other antimicrobial peptide genes in GenBank. (ukessays.com)
2) The influence of the peptide sequence and aromatic core of the building blocks. (europa.eu)
Our work implies that the rich structural information extracted from fragment libraries can complement sequence-derived features to help protein structure prediction. (biomedcentral.com)

These findings are in accord with the previous data in which it had been shown that substitution of these aromatic residues by aliphatic amino acids leads to loss of binding of the modified peptide with alpha-BTX. (rcsb.org)
Using 2C6 and variants, alanine scanning mutagenesis identified three amino acids (aa 592, 595, and 596) in the overlapping region of the previously identified peptide. (nih.gov)
When we mapped these amino acids on the trimerized structure, they spanned across oligomers, supporting the notion that the epitope targeted by 2C6 lies in a recessed pocket between two gp41 oligomers. (nih.gov)
We can synthesise difficult peptides (greater than 100 amino acids) and peptides with a wide and increasing range of amino acid modifications including N-terminal, C-terminal and side-chain modifications, multiple disulphide bridges, fluorescent labels, lipidation, D-amino acid and heavy isotope incorporation, biotinylation, glycosylation, post translational modifications, PEGylation and more. (2bscientific.com)
Amino acid analysis can be provided as an additional service to determine peptide composition, and can also be used to determine net peptide if required. (2bscientific.com)
SPIDR was tested using the potent and selective 16-amino acid peptide α -conotoxin MII ligand and the α 3 β 2 -nicotinic acetylcholine receptor (nAChR) isoform. (biomedcentral.com)
Here we are investigating the role of the histidines in more detail by defining a library of variants of the peptide, where the former have been replaced by the pH-insensitive amino acid glutamine. (lu.se)
PDZ domains consist of 80 to 90 amino acids comprising six beta-strands (beta-A to beta-F) and two alpha-helices, A and B, compactly arranged in a globular structure. (embl.de)
The isolated PDZ domain (amino acids 206-334) is capable of folding into a well-behaved structure and binds to a nonpolar peptide with a dissociation constant (K(D)) of 1.9 microM, similar to that of the intact Tsp protein. (embl.de)
Second, peptide stability: peptides can potentially be cut by enzymes (proteases) into smaller and useless fragments or even single amino acids. (epfl.ch)
In addition, the chemists systematically changed the amino acids between the cysteines and generated libraries of billions of different double-bridged peptides. (epfl.ch)
The three-dimensional structure of 2H1, a protective monoclonal antibody to Cryptococcus neoformans, has been solved at 2.4 Å resolution, in both its unbound form and in complex with the 12 amino acid residue peptide PA1 (GLQYTPSWMLVG). (elsevierpure.com)
Once they had isolated the protein, the team set about adjusting it to explore which changes to its structure might alter its function, by fiddling with the amino acids in its 20-amino-acid chain. (bioedonline.org)
In peptides made from canonical amino acids, positively charged side chains occupy a separate solvated shell from negatively charged ones (left). (acs.org)
But noncanonical amino acids with shorter chains such as 2,4-diamino-butyric acid can form salt bridges with negatively charged sidechains, disrupting protein structure (right). (acs.org)
Starting from a core of seven early amino acids, Klára Hlouchová of Charles University, Stephen D. Fried of Johns Hopkins University, and coworkers added sets of other amino acids, made peptide libraries from each set, and compared them with peptides made from 19 of the canonical amino acids. (acs.org)
Some of the peptide libraries were made of smaller sets of the canonical amino acids. (acs.org)
Two of the libraries included noncanonical-but prebiotically available-amino acids. (acs.org)
For instance, in epitope mapping experiments, peptides should contain between 9 and 22 amino acids because epitopes typically fall within that size range. (proteogenix.science)
In practice, this parameter is defined by the number of amino acid residues shared by neighboring peptides. (proteogenix.science)
A collection of cloned peptides, or chemically synthesized peptides, frequently consisting of all possible combinations of amino acids making up an n-amino acid peptide. (bvsalud.org)
Hepcidin, a 25-amino acid peptide synthesized in liver, is also known as the "iron hormone. (medscape.com)

Lack of overlapping peptide binding supported a nonlinear epitope structure. (nih.gov)
The kinetics and products of degradation of HIV-1 Gag favored the production of peptides encompassing the immunodominant epitope and destruction of the subdominant one. (jci.org)
the modulation of epitope immunodominance and the processing and presentation of HIV peptides for MHC class I recognition were shown to be dependent on flanking residues that were N terminal to the natural epitopes (see the related article beginning on page 3563). (jci.org)
Poor steric complementarity between PA1 and the antibody heavy chain along with the fact that the majority of the interactions between 2H1 and PA1 involve van der Waals interactions with the light chain may explain why this peptide acts as only a partial mimotope of the capsular polysaccharide epitope. (elsevierpure.com)
It is widely used in peptide library screening for drug discovery, target validation, epitope mapping, vaccine development, and structure-activity studies. (novoprolabs.com)

Peptide library scanning showed consistent binding in this region but to only a single peptide. (nih.gov)
Partial proteolysis by V8 protease at a single peptide bond immediately N-terminal to the PDZ domain resulted in two distinct and relatively stable fragments and complete loss of catalytic activity. (embl.de)

Hundreds of Peptides - 1 to 5 mg unpurified (desalted) Ideal size 5 to 20aa, up to 60. (2bscientific.com)
In this case, instead of screening hundreds of peptides individually, researchers can focus on identifying positive peptide pools. (proteogenix.science)

The structure of the complex may explain the fact that the library-derived peptide binds alpha-BTX with a 15-fold higher affinity than that shown by the acetylcholine receptor peptide (alpha185-196). (rcsb.org)
HLA-DR molecules were affinity purified and bound peptides acid eluted. (jci.org)
Molecular features of ligand binding to MHC class II HLA-DR molecules have been elucidated through a combination of peptide structure-activity studies and structure-based drug design, resulting in analogues with nanomolar affinity in binding assays. (rcsb.org)
The peptides that exhibited the highest affinity for α 3 β 2 -nAChR were used as models for a small-molecule structure similarity search of the PubChem Compound database. (biomedcentral.com)
Appropriate cassettes can be selected and conjugated to produce longer peptides, in which constraints impart appropriate helicity such that a wide range of targets can be engaged with high affinity and selectivity. (bath.ac.uk)
They show high binding affinity, low toxicity, and are easy to synthesize, all of which makes peptides ideal for use in drug development, and many naturally occurring peptides such as insulin, oxytocin, somatostatin and the antibiotics vancomycin or polymyxin B, are already successfully used like that. (epfl.ch)
After screening the libraries, the researchers were able to isolate high-affinity binders to important protein targets. (epfl.ch)
A small number of H-bonds between peptide and antibody contribute to the affinity and specificity. (elsevierpure.com)

A peptide library is a systematic combination of a large number of different peptides. (novoprolabs.com)
To overcome this limitation, researchers often group different peptides into pools. (proteogenix.science)

However, even when using GPF, these tools still generally detect fewer peptides than library search engines, which can leverage previously acquired instrument-specific fragmentation and measured retention times. (nature.com)
When offset numbers are high and peptides long, the resulting library will have fewer peptides. (proteogenix.science)

The molecule, known as VIRIP (virus-inhibitory peptide), binds to a spiky protein on the surface of the HIV virus called gp41. (bioedonline.org)
describes the use of a phage-displayed combinatorial random peptide library to identify a 27-residue peptide that binds to C3 and inhibits complement activation. (justia.com)

Cyclization of peptides with two chemical bridges affords large scaffold diversities. (epfl.ch)

Based on the the conserved sequences of antimicrobial peptides of insects (mainly Diptera) from different antimicrobial peptide databases, thirty-seven pairs of degenerate primers were designed, and the antimicrobial peptide genes from Hermetia illucens L were screened using these primers. (ukessays.com)
Seven gene fragments for three types of antimicrobial peptides cecropin, sarcotoxin and stomoxyn were acquired. (ukessays.com)
Currently, the need for safe and effective antimicrobial peptides agents increases in parallel with the emergence of many antibiotic-resistant strains as a consequence of excessive use and widespread of the antibiotics [1]. (ukessays.com)
Many desirable features, such as low cost of production, rapidity, heat-tolerant, relatively broad antimicrobial spectrum and low toxicity to eukaryotic cells made of antimicrobial peptides to be a new alternative to the conventional antibiotic [2]. (ukessays.com)
The insects immune systems possess several features in common, does not have specific immune system as found in the higher animals , lacking of B and T lymphocytes and no immunoglobulins and complement production [3], this make insect developed effective and complex innate immune and antimicrobial peptides are a key factor of the insect's immune system [ 4 , 5 ]. (ukessays.com)
Antimicrobial peptides (AMPs) have a variety of interesting biological functions including antibacterial, antifungal, antiparasitic, antitumoral, and antiviral activities [6-8]. (ukessays.com)
Nowadays, molecular biology researchs are featured a wide range of antimicrobial peptides (AMP)-related topics such as AMP genome library construction, development of antimicrobial peptides and their mimetics as therapeutic agents, structure/function analysis, cloning and expression, regulation, mechanisms of action, microbial escaping strategies and human disease associations. (ukessays.com)

To accomplish this, precursor isolation windows are widened such that multiple peptides are usually co-fragmented in the same MS/MS scan. (nature.com)
The building blocks, which carry only one peptide, do not self-associate, while sufficiently large macrocycles, which display multiple peptides, will self-assemble into extended tubular structures. (europa.eu)

In this work, protein-multipeptide constructs composed of anti-immunocomplex peptides selected from phage libraries and streptavidin/avidin as core protein were used for direct detection of small compounds in a noncompetitive two-site immunoassay format that performs with increased sensitivity and positive readout. (cdc.gov)

Recently it has become possible to accurately predict spectra from peptide sequences 14 , 15 , but direct searching of single-injection DIA data has remained problematic, in part due to the false discovery rate (FDR) correction required when considering all possible tryptic peptides in a FASTA database. (nature.com)

This peptide was truncated to a 13-residue cyclic segment that maintained complete activity, which is referred to in the art as compstatin (SEQ ID NO: 2J. (justia.com)
NANOTUBES formed from cyclic peptides (PEPTIDES, CYCLIC). (uams.edu)

Crystal structures of four ternary complexes of different peptide mimetics with the rheumatoid arthritis-linked MHC DRB10401 and the bacterial superantigen SEB have been obtained. (rcsb.org)
These studies illustrate the complementary roles played by phage display library methods, peptide analogue SAR, peptide mimetics substitutions, and structure-based drug design in the discovery of inhibitors of antigen presentation by MHC class II HLA-DR molecules. (rcsb.org)

Subsequently, peptides were separated by reversed-phase HPLC and analyzed by liquid chromatography-mass spectrometry. (jci.org)
The majority of peptides are in freeze dried form and will contain counter ions and physically bound water as a result of cleavage from resin, hplc and drying. (2bscientific.com)
Peptide and biochemical purity is assessed using an array of techniques including HPLC, NMR, microanalysis, high voltage electrophoresis, TLC, and mass spectrometry as appropriate. (2bscientific.com)
Peptides purified by reversed phase HPLC or ion exchange chromatography have their purity given as the area under the curve of the major peak when analysed at a specific wavelength, usually 230nm. (2bscientific.com)
Stringent quality control: NovoPro provides Certificate of Analysis, MS, and HPLC validation data for each peptide. (novoprolabs.com)
All the peptides synthesized are controlled by MS and HPLC. (proteogenix.science)

Plasma cells produce antibodies (eg, rheumatoid factor [RF], anticyclic citrullinated peptide [anti-CCP] antibody) that contribute to these complexes, but destructive arthritis can occur in their absence. (msdmanuals.com)

Gas-phase fractionation 12 (GPF) improves detection rates with these tools 10 by injecting the same sample multiple times with tiled precursor isolation windows, allowing each injection to have narrower windows (and thus fewer co-fragmented peptides) with the same instrument duty cycle. (nature.com)
This 18 F-azadibenzocyclooctyne (ADIBO-F) was radiolabelled from its toluenesulfonate precursor in 21-35 % radiochemical yields, and subsequently incorporated into two cancer-targeting peptides through SPAAC chemistry. (uwo.ca)
We further demonstrate the utility of CFB to rapidly generate and characterize multisite precursor peptide variants to evaluate the substrate tolerance of the biosynthetic pathway. (illinois.edu)

While this method is often prohibitively expensive because it requires enough instrument time and protein content for multiple injections for each sample, the use of multiple GPF injections can be applied just to pooled samples to generate DIA-only chromatogram libraries that make it easier to detect peptides in single-injection DIA experiments 13 . (nature.com)
In this article, a powerful Python library is presented, to quickly and efficiently generate realistic peptide model structures. (peerj.com)
A simple Python library to generate model peptides. (openwetware.org)
We used DeepFragLib, the state-of-the-art fragment library construction approach when benchmarked on recent CASPs [ 21 ], to generate fragment libraries for subsequent studies. (biomedcentral.com)

This approach can be extended to characterize HLA-bound peptides in various autoimmune settings. (jci.org)

Some lasso peptides have been shown to bind human cell-surface receptors and exhibit anticancer properties, while others display antibacterial or antiviral activities. (illinois.edu)
And these open the door widely to screening identifying and characterizing new antibacterial peptides. (ukessays.com)

A combinatorial library/high throughput screening methodology was designed to optimize translocation of the nascent peptide into the endoplasmic reticulum (ER) and protein folding within the ER. (sbir.gov)

The saga of MHC-bound peptides: a renaissance for antigen presentation? (jci.org)
Peptide and peptide mimetic inhibitors of antigen presentation by HLA-DR class II MHC molecules. (rcsb.org)

The peptide fills an alpha-BTX pocket made of residues located at fingers I and II, as well as at the C-terminal region. (rcsb.org)
Of the peptide residues, the largest contact area is formed by Tyr-3 and Tyr-4. (rcsb.org)
Although smaller libraries are much easier and less expensive to synthesize, the likelihood of researchers finding the precise interface residues in complex projects is often limited. (proteogenix.science)
In contrast, when offset numbers are low and peptides short, the resulting library will have more peptides, making it easier to identify the interface residues but also resulting in more time-consuming experiments. (proteogenix.science)

This thesis will document a method of combinatorial chemistry to construct libraries of new potential peptide-based imaging agents for positron emission tomography (PET) imaging. (uwo.ca)
It will also incorporate the study of strain-promoted alkyne-azide cycloaddition (SPAAC) as a type of copper-free click chemistry used as a method for radiofluorination of biological structures. (uwo.ca)
Each peptide in the library contains a single fluorine atom incorporated through established SPAAC chemistry. (uwo.ca)
I want to get the latest chemistry news from C&EN in my inbox every week. (acs.org)
from the technical review on the medicinal chemistry and molecular pharmacology of opioid peptides and the opiates which took place on September 4-6, 1984, at Bethesda, Maryland"--Page iv. (epa.gov)
We will make dynamic combinatorial libraries of macrocyclic molecules of different ring sizes that can exchange building blocks through reversible covalent chemistry. (europa.eu)

While they are conceptually inspired by biology, the systems that were developed are much simpler in composition, and combine biological and synthetic components, giving rise to structures and functions, including adaptive fluorescence emission, that are not accessible using biological or synthetic approaches alone. (dtic.mil)
Peptide libraries are collections of synthetic peptides, invaluable for studying protein-protein interactions. (proteogenix.science)

The bound peptide adopts an almost-globular conformation resulting from three turns that surround a hydrophobic core formed by Tyr-11 of the peptide. (rcsb.org)

This software contains an intuitive graphical interface for peptide mutant screening workflow and facilitates mapping, clustering, and searching of local molecular databases, making DockoMatic a valuable tool for researchers in drug design and repurposing. (biomedcentral.com)
Using this strategy, the researchers produced an enormous diversity of peptide structures, all with different "skeleton" structures. (epfl.ch)
Through screening and profiling applications, libraries help researchers determine relevant antigen-antibody recognition sites, unravel enzyme-substrate specificity, or determine the sites responsible for receptor binding - particularly useful when studying pathogenic viruses. (proteogenix.science)
By mimicking the natural structure of peptides from the protein thrombin, researchers have designed a peptide-based treatment, sHVF18. (lu.se)

Small-molecule Peptide-Influenced Drug Repurposing (SPIDR) was developed to identify small molecule drugs that target a specific receptor by exploring the conformational binding space of peptide ligands. (biomedcentral.com)

Examples include chemical compounds including drugs, nucleotides including siRNAs and miRNAs, carbohydrates, lipids, peptides and chemically-modified macromolecules. (mcmaster.ca)

Cost-Utility Analysis of Glucagon-Like Peptide-1 Agonists Compared with Dipeptidyl Peptidase-4 Inhibitors or Neutral Protamine Hagedorn Basal Insulin as Add-On to Metformin in Type 2 Diabetes in Sweden. (lu.se)

Although both peptides bind to similar sites on alpha-BTX, the latter adopts an extended conformation when bound to the toxin [Basus, V., Song, G. & Hawrot, E. (1993) Biochemistry 32, 12290-12298], whereas the library peptide is nearly globular and occupies a larger surface area of alpha-BTX binding site. (rcsb.org)

Site-directed mutagenesis of a surface residue at the peptide binding site of the PDZ domain, valine 229, to Glu or Gln resulted in an increase in the K(M) value but had no effect on the k(cat) value. (embl.de)
The toolkit created in Phase I will be expanded through the implementation of several new high throughput approaches toward yield improvement including mutagenesis and cDNA library screening and deletion of protease genes as well as traditional fermentation and purification process development. (sbir.gov)

These materials mimic the ability of living systems to rapidly adapt and respond to new situations by changing their structure and optical properties. (dtic.mil)

SPIDR identified non-peptide drugs with estimated binding affinities nearly double that of the native α -conotoxin MII peptide. (biomedcentral.com)
However, using peptides as drugs faces two challenges. (epfl.ch)
The structure of C3c in complex with the complement inhibitor, compstatin, and use of this information for rational design or identification of complement-inhibiting drugs are disclosed. (justia.com)
There is an increasing demand for peptide libraries from pharmaceutical companies due to the expanding interest in peptide drugs. (novoprolabs.com)

Because compstatin blocks this critical step of complement activation and because it is a small non-immunogenic peptide, compstatin has the potential to be developed into a therapeutic agent. (justia.com)
As a complement to our peptide library services, NovoPro provides 6 free peptide library design tools to support research projects from structural biology to vaccine development. (novoprolabs.com)
However, those features only leverage sequential information, and incorporating new features from known protein structures could serve as a complement and thus benefit protein property predictions. (biomedcentral.com)

They screened a massive library of compounds isolated from 10,000 litres of filtered human blood, looking for substances that could naturally inhibit HIV. (bioedonline.org)
High-throughput capacity: More than 10,000 peptides/month. (novoprolabs.com)

Our method specifically enables rapid, experiment-specific library generation for non-model organisms, which we demonstrate using the malaria parasite Plasmodium falciparum , and non-canonical databases, which we show by detecting missense variants in HeLa. (nature.com)

This graph shows the total number of publications written about "Nanotubes, Peptide" by people in UAMS Profiles by year, and whether "Nanotubes, Peptide" was a major or minor topic of these publications. (uams.edu)
Below are the most recent publications written about "Nanotubes, Peptide" by people in Profiles over the past ten years. (uams.edu)
Below are the most recent publications written about "Amyloid beta-Peptides" by people in Profiles. (rush.edu)

The solution structure of the complex between alpha-bungarotoxin (alpha-BTX) and a 13-residue library-derived peptide (MRYYESSLKSYPD) has been solved using two-dimensional proton-NMR spectroscopy. (rcsb.org)
High Resolution Structure of a Calmodulin Rs20 Peptide Complex. (expasy.org)
We first designed alanine mutants based on the Evasin-1·CCL3 complex structure and an in silico model of Evasin-4 bound to CCL3. (unige.ch)
Our partner has the technology to synthesize 96 complex peptides in parallel and offers various grades of quality controls. (2bscientific.com)
In particular, this invention provides the structure of the C3 inhibitor, compstatin, in complex with C3c, and uses of that structural information for rational drug design. (justia.com)

The peptides' highly efficient binding, combined with their small size and high stability make them ideal for drug therapies. (epfl.ch)
Stability was also a highly attractive feature of the new peptide format. (epfl.ch)
99mTc- and 188Re-labeled peptides displayed high labeling efficiency and favorable stability in saline and human plasma. (phoenixpeptide.com)

For the first time, to our knowledge, we have identified HLA-bound peptides presented in vivo during an inflammatory condition. (jci.org)
This method can detect all seven known BoNT serotypes and involves incubating BoNT with a peptide substrate that mimics each toxin's natural in vivo neuronal protein target. (cdc.gov)

Subsequently, supramolecular structures became visible by microscopy, revealing amyloid-like fibrils composed of both the activating peptide and PLA2. (uu.nl)

Our partner can synthesize peptides from 2 to 120 aa, mg to multi-grams at various purity grade. (2bscientific.com)

Glu-5 and Leu-8, which also remarkably contribute to the contact area with the toxin, are present in all the library-derived peptides that bind strongly to alpha-BTX. (rcsb.org)
EPFL scientists have developed a new type of "double-bridged peptide" that can be tailored to bind tightly to disease targets of interest. (epfl.ch)
Using the double-bridge approach for both these protein targets, the scientists developed peptides that could bind them efficiently at nanomolar concentrations. (epfl.ch)
In the case of kallikrein, the peptides could bind it for more than an hour before dissociating. (epfl.ch)

Peptide binding is associated with only minor rearrangements of some side-chains and a small shift in the H2 loop of the antibody. (elsevierpure.com)
The peptide assumes a tightly coiled conformation consisting of one inverse γ-turn and one type II β-turn that serves to place the entire peptide motif, consisting of Thr(P5) Pro(P6), Trp(P8) Met(P9) and Leu(P10), into a depression in the antibody combining site. (elsevierpure.com)

It also provides 30,000 independent fold structures produced by this method and made available as a microarray on a substrate. (genomeweb.com)

Here we are investigating the role of the histidines in more detail by defining a library of variants of the peptide, where. (lu.se)
However, it was shown to affect the penetration depth of the peptide into the bilayer, where all variants except the one with zero histidines were found below the bilayer. (lu.se)
These data lay a firm foundation for the creation of large lasso peptide libraries using CFB to identify new variants with unique properties. (illinois.edu)

Prearranged positive allosteric modulators (PAMs) inspired by photopharmacology are synthesized and applied to the glucagon-like peptide-1 receptor (GLP-1R), a class B G protein-coupled receptor. (wiley.com)

SPIDR incorporates a genetic algorithm-based, heuristic search procedure, which was used to explore the ligand binding domain of the α 3 β 2 -nAChR isoform using a library consisting of 640,000 α -conotoxin MII peptide analogs. (biomedcentral.com)
Peptide binding of the ligand takes place in an elongated surface groove as an anti-parallel beta-strand interacts with the beta-B strand and the B helix. (embl.de)

Photoaffinity labeling with a fluorescent nonpolar peptide caused the covalent attachment of the peptide to a single site on the Tsp protein. (embl.de)

We hypothesize that this is due to the ability of the histidines to charge titrate, which charges up the peptide, and enables it to penetrate and translocate through the lipid bilayer. (lu.se)
Proposed Commercial Application: GlycoFi's Humanized Yeast(tm) enables the study of glycosylation structure-function relationships and creates the ability not only to pinpoint the specific glycoforms that optimize the bioactivity, pharmacokinetics and overall efficacy of a therapeutic protein, but also to manufacture them at low cost with a high degree of uniformity. (sbir.gov)
Descriptors are arranged in a hierarchical structure, which enables searching at various levels of specificity. (uams.edu)

Ingestion of a collagen peptide containing high concentrations of prolyl-hydroxyproline and hydroxyprolyl-glycine reduces advanced glycation end products levels in the skin and subcutaneous blood vessel walls: a randomized, double-blind, placebo-controlled study. (medlineplus.gov)

Owing to a locked three-dimensional structure, lasso peptides can be unusually stable toward heat and proteolytic degradation. (illinois.edu)

In this issue of the JCI , two separate studies on MHC-bound peptides reopen the debate on the utility of peptides for the purposes of vaccination and treatment of autoimmune diseases. (jci.org)
peptides bound to HLA-DR17 from bronchoalveolar lavage cells of sarcoidosis patients were analyzed in order to identify target antigens of the autoimmune response (see the related article beginning on page 3576). (jci.org)
Structures of a significant number of SH2 domains both in isolation and bound to various target molecules have been determined by X-ray crystallography and NMR spectroscopy. (lu.se)

Ideally, this number should be 1/3 of the full length of the peptide fragments, to ensure all regions of the protein segment are equally covered. (proteogenix.science)
Bioinformatic analysis was done to predict the secondary structure and three-dimensional structure of these seven gene fragments that may exist. (ukessays.com)
Fragment libraries play a key role in fragment-assembly based protein structure prediction, where protein fragments are assembled to form a complete three-dimensional structure. (biomedcentral.com)
For over two decades, Artur Schmidtchen and his research group have studied endogenous peptides, smaller protein fragments in the body with the ability to both kill bacteria and neutralize the bacterial toxins released during an infection. (lu.se)

The double-bridged peptides are hardly degraded by proteases in the blood, which is a great advantage because it keeps the peptides from being eliminated too quickly, thus extending their therapeutic effects. (epfl.ch)

Compstatin (SEQ ID NO:2), a 13-residue peptide, circularized by disulfide bond (Cys-2-Cys-12), displays an inhibitory activity of IC 50 =12 μM. (justia.com)

It was the overall aim of this research program to develop a new class of transient functional nanostructures based on self-assembling peptides. (dtic.mil)

Crystal structure and mutational analysis of the Saccharomyces cerevisiae cell cycle regulatory protein Cks1: implications for domain swapping, anion binding and protein interactions. (expasy.org)
We expect to be able to tune the size of the replicating macrocycle by altering the strength of the interactions between the peptides. (europa.eu)

Data-independent acquisition approaches typically rely on experiment-specific spectrum libraries, requiring offline fractionation and tens to hundreds of injections. (nature.com)
Most importantly, these libraries are built at the expense of time, sample, and considerable effort with offline fractionation, especially considering that they are typically not reusable across laboratories or instrument platforms 9 . (nature.com)

Lasso peptides are ribosomally synthesized and post-translationally modified peptide (RiPP) natural products that display a unique lariat-like, threaded conformation. (illinois.edu)

however, the discovery, isolation, and characterization of lasso peptides are constrained by the lack of an efficient production system. (illinois.edu)

Anatomy9

Organisms9

Chemicals and Drugs51

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes37

Disciplines and Occupations2

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science20

Health Care2

Proteins10

Synthesis13

Sequence14

Amino22

Epitope5

Single peptide2

Hundreds of Peptides2

Affinity8

Different peptides2

Fewer peptides2

Binds2

Cyclization1

Antimicrobial peptides7

Multiple peptides2

Phage libraries1

Sequences1

Cyclic2

Mimetics2

HPLC6

Antibodies1

Precursor3

Generate4

Characterize1

Antibacterial2

Combinatorial library1

Antigen presentation2

Residues4

Chemistry6

Synthetic2

Hydrophobic1

Researchers4

Ligands1

Chemically modified1

Inhibitors1

Globular1

Mutagenesis2

Mimic1

Drugs4

Complement3

10,0002

Rapid1

Profiles3

Complex5

Stability3

Vivo2

Subsequently1

Synthesize1

Bind4

Antibody2

Substrate1

Variants3

Receptor1

Ligand2

Covalent1

Enables3

Concentrations1

Degradation1

Bound3

Fragments4

Therapeutic1

Disulfide1

Nanostructures1

Interactions2

Typically2

Conformation1

Characterization1