• Correspondence: Jan E. Brinchmann, M.D., Ph.D., Norwegian Center for Stem Cell Research and Institute of Immunology, Oslo University Hospital-Rikshospitalet, P.O. Box 1121 Blindern, 0317 Oslo, Norway. (wiley.com)
  • We used IL-4 and IL-13 dual-reporter mice to show that naive CD4 + T cells cultured in the presence of IL-4 and thymic stromal lymphopoietin (TSLP) generate a population of IL-4 neg IL-13 pos Th2 cells that develop from IL-4 neg precursors and express the Th2 effector cytokines IL-5 and IL-9. (pnas.org)
  • Here we report that a substantial proportion of naive CD4 + T cells in spleen and lymph node express receptors for the epithelium-derived inflammatory cytokine thymic stromal lymphopoietin (TSLP). (pnas.org)
  • Thymic stromal lymphopoietin (TSLP) is an important factor responsible for the pathogenesis of allergic diseases, such as atopic dermatitis and asthma. (mdpi.com)
  • Moon P-D, Han N-R, Lee JS, Kim H-M, Jeong H-J. Effects of Linalyl Acetate on Thymic Stromal Lymphopoietin Production in Mast Cells. (mdpi.com)
  • Thymic stromal lymphopoietin (TSLP) signals via a receptor comprising the interleukin (IL)-7 receptor α chain and a distinctive subunit, TSLP receptor (TSLPR), which is most related to the common cytokine receptor γ chain, γ c . (rupress.org)
  • Like IL-7 KO mice, IL-7Rα KO mice exhibit severely reduced thymic cellularity and defective T cell maturation ( 2 , 3 ). (rupress.org)
  • Thymic stromal lymphopoietin (TSLP) is a cytokine that was originally identified as a growth factor in the supernatant of the Z210R.1 thymic stromal cell line that could support the development of immature NAG8/7 B cells to the B220 + /IgM + stage ( 5 ). (rupress.org)
  • In addition, daily injection of TSLP into γ c KO mice increased both thymic and splenic cellularities by enhancing the expansion of both T and B cells, with the accumulation of CD4 + T cells in the periphery. (rupress.org)
  • Seeding of thymic microenvironments defined by distinct thymocyte-stromal cell interactions is developmentally controlled. (biomedsearch.com)
  • Thymic stromal lymphopoietin (TSLP) is an interleukin (IL)-7-like cytokine that triggers dendritic cell-mediated T helper (Th)2 inflammatory responses through a receptor consisting of a heterodimer of the IL-7 receptor alpha (IL-7Ralpha) chain and the TSLP receptor (TSLPR), which resembles the cytokine receptor common gamma chain. (unboundmedicine.com)
  • Allergen exposure primes IL-4 + Th2 cells in lymph node, but production of effector cytokines including IL-5 and IL-13 is thought to require additional signals from antigen and the environment. (pnas.org)
  • In addition to organizing T and B cell segregation and expressing lymphocyte survival factors, stromal cells support the migration and interactions between antigen presenting cells and naïve T and B cells during the initiation of immune responses and influence the outcome between tolerance and immunity. (keystonesymposia.org)
  • Recombinant fragment, corresponding to a region within amino acids 1-146 of Human Bone marrow stromal cell antigen 1. (abcam.com)
  • Flow cytometric analysis revealed that pMSC expressed surface antigens also found on hMSC, including CD90, MSCA-1 (TNAP/W8B2 antigen), CD44, CD29 and SLA class I. Clonogenic outgrowth was significantly enriched following selection of CD271+ cells from BM of human and pig (129 ± 29 and 1961 ± 485 fold, respectively). (wiley.com)
  • In contrast, CD14, CD79α and the embryonic stem cell markers Oct-4, SSEA (stage specific embryonic antigen) -1, -3, -4, TRA (tumor rejection antigen) -1-60 and -1-81 are not expressed. (dovepress.com)
  • There are currently no images for Bone marrow stromal cell antigen 1/CD157 Antibody (AF4710). (novusbio.com)
  • CD157, also known as bone marrow stromal cell antigen 1 (BST-1), is a glycosyl phosphatidylinositol anchored membrane protein that belongs to the CD38 family (1). (novusbio.com)
  • The majority of TSLPR(+) cells were DC co-expressing blood DC antigen-1 (BDCA-1) or BDCA-2. (unboundmedicine.com)
  • Injection of S. typhimurium antigen complex to mice CBA increased 5 times colony forming efficiency (CFE) and, respectively, content of stromal precursor cells (CFU-F) in femur bone marrow and 9 times in spleen of these animals with maximum at the first day. (scirp.org)
  • Overall, our findings suggest that CCL21 promotes TLOs that differ from inflammatory TLOs found in T1D islets in that they resemble lymph nodes, contain FRC-like cells expressing β-cell autoantigens and are able to induce systemic and antigen-specific tolerance leading to diabetes prevention. (diabetesjournals.org)
  • The present disclosure relates to antigen specific tolerogenic antigen presenting cells presenting antigenic portions of an autoantigen and to related. (patents.com)
  • For many years, NOD scid (NOD.CB17- Prkdc scid /J, 001303 ) and nude (NU/J, 002019 or J:NU, 007850 ) mice have commonly been used as hosts for cancer cell lines in basic and therapeutic research. (jax.org)
  • The Il2rg null mutation in NSG and NRG mice prevents functional development of NK cells, allowing engraftment of a wide range of human PDX. (jax.org)
  • The host mice maintained normal hemoglobin and hematocrit 14 days after the start of 5-FU therapy, but, as expected, showed a drop in white blood cells (WBC) count. (jax.org)
  • Bone marrow cell suspensions were prepared from male B6C3F1-mice and cultured with hydroquinone (123319), benzoquinone (106514), catechol (120809), phenol (108952), and 1,2,4-benzenetriol (533733) at concentrations from 0.0000015 to formed in the presence of these metabolites was determined and compared with values from untreated stromal cell cultures. (cdc.gov)
  • How the transplanted bone marrow stromal cells restore the lost neurologic function is not clear," added the co-author of "Improved Expression of ã-Aminobutyric Acid Receptor in Mice With Cerebral Infarct and Transplanted Bone Marrow Stromal Cells: An Autoradiographic and Histologic Analysis. (innovations-report.com)
  • however, this up-regulated caspase-1 activity was down-regulated by LA. Finally, LA decreased intracellular calcium levels in HMC-1 cells as well as PMA-induced ear swelling responses in mice. (mdpi.com)
  • Primary white preadipocytes isolated from white adipose tissues in mice can be differentiated into beige/brite cells. (jove.com)
  • Injection of TSLP into γ c KO mice induced the expansion of T and B cells. (rupress.org)
  • IL-7 plays a critical nonredundant role in the development of T and B lymphocytes in mice ( 2 , 3 ) and of T cells in humans ( 4 ). (rupress.org)
  • These mice exhibited normal T and B cell development and cellularity. (rupress.org)
  • Seeding of distinct intrathymic microenvironments defined by direct thymocyte-stromal cell interactions was correlated with T cell development in situ using radiation and nonradiation chimeras of Thy-1.1/1.2 congenic mice. (biomedsearch.com)
  • We explored the steady-state stromal composition of lymph nodes isolated from mice and humans, and found that marginal reticular cells and lymphatic endothelial cells required lymphocytes for their normal maturation in mice. (harvard.edu)
  • In this study, human breast cancer cells (MCF-7) were established in mice creating a tumor with species-specific cancer and stroma cells. (diva-portal.org)
  • In subcutaneous Matrigel plugs in mice, ENL and ENL+GEN decreased E2-induced endothelial cell infiltration, whereas GEN alone did not. (diva-portal.org)
  • Analysis of autoradiographs showed T cells from normal or immunized mice distributing equally to red pulp and white pulp at 1 hr after injection of isolated radioactively labeled T cells, but they increased in white pulp over time. (ajtmh.org)
  • A higher percentage of immune cells was found in the white pulp of mice on day 16 of infection, suggesting a role for these cells in the development of crisis. (ajtmh.org)
  • Gorskaya, U. , Danilova, T. , Mezentseva, M. , Shapoval, I. and Nesterenko, V. (2011) Effect of antigens on colony forming efficiency of stromal clonogenic cells and expression of cytokine genes in primary cultures of bone marrow and spleen of mice. (scirp.org)
  • Ins2-CCL21 transgenic, non-obese diabetic (NOD) mice express CCL21 in pancreatic β-cells and do not develop autoimmune diabetes. (diabetesjournals.org)
  • Ins2-CCL21 mice develop TLOs by 4 weeks of age consisting of naïve CD4 + T cells compartmentalized within networks of CD45 - gp38 + CD31 - fibroblastic reticular cell (FRC)-like cells. (diabetesjournals.org)
  • Consistently, transgenic mice harbored fewer autoreactive T cells and higher proportion of Tregs in the islets. (diabetesjournals.org)
  • Using transgenic mice expressing an SDF-1-mRFP1 (monomeric red fluorescence protein 1) fusion protein we observed that SDF-1 was localized in synaptic vesicles in the terminals of basket cells together with GABA-containing vesicles. (jneurosci.org)
  • In addition, we demonstrate that concurrent deletion of Tgfbr2 in stromal and nephrogenic cells in vivo results in decreased nephron formation and an expanded nephrogenic precursor domain similar to that observed in Smo -deficient mutant mice. (biologists.org)
  • In vivo, high TSLP levels promote the development of a similar population of IL-4 neg IL-13 pos T cells that also express Gata3 , Il5 , and Il3 transcripts. (pnas.org)
  • In vivo, high TSLP levels acted directly on CD4 + T cells to induce the development of IL-13-SP and IL-4 + IL-13 + double-positive populations in lymph node. (pnas.org)
  • Furthermore, RA-producing LP SCs and intestinal DCs localized closely in vivo suggesting that the interactions between both cell types might have an important role in the functional education of migratory DCs and therefore in the regulation of immune responses toward oral and commensal antigens. (nih.gov)
  • Osteoblasts, the bone-forming cells, derive from multipotential bone marrow stromal precursors called colony-forming units-fibroblastic (CFU-F). CFU-F rapidly adhere to plastic upon culture ex vivo, adhesion of such stromal precursors to bone in vivo is likely to be an early event in the anabolic response to bone stimulatory factors. (bl.uk)
  • Reversion of the malignant phenotype of human breast cells in three-dimensional culture and in vivo by integrin blocking antibodies. (springer.com)
  • In vivo, the consistent results were also found: PZ-old cells promoted prostate cancer cells growth, but this effect receded when knocking down ARA55 expression in PZ-old cells. (urotoday.com)
  • 1 CFU-F initiating cells in vivo have been shown to be quiescent, existing at a low frequency in human bone marrow. (stemcell.com)
  • This invention provides ex vivo methods for making modified natural killer cell compositions having overall anti-fugetactic properties for the effective and. (patents.com)
  • These cells were evaluated for the effects of in vivo hyperglycemia on the survival and function of mesenchymal progenitors. (medsci.org)
  • T helper 2 (Th2) cells are defined by their ability to produce the hallmark cytokine IL-4. (pnas.org)
  • Conclusions: Results demonstrate that FL is produced spontaneously by BM stromal cells and that FL production is regulated by cytokine stimulation but not by ionizing radiations. (irsn.fr)
  • Two major types of stromal cells related to early cancer development, cell lines HS5 and HS27a, are helpful in studying tumor cell growth and stromal interactions by deciphering crosstalk between stromal cells and clonal cells. (thermofisher.com)
  • Peritoneal injection of EdU resulted in the appearance of EdU-positive cells in most organs and tissues. (nih.gov)
  • In the intestine, EdU-positive cells were found in both the epithelium and connective tissues 7 h after injection. (nih.gov)
  • However, to mediate allergic inflammation in tissues, Th2 cells must secrete additional cytokines including IL-13 and IL-5. (pnas.org)
  • Stromal cells also play instrumental roles in coordinating immune responses in non-lymphoid tissues, in inflammatory and autoimmune diseases, and in chronic infection. (keystonesymposia.org)
  • The NSG-GFP hosts express green fluorescent protein in most cells and tissues. (jax.org)
  • The comprehensive understanding of tumour-associated normal cells in tumour tissues may provide important insights into tumour biology and aid in the development of robust prognostic and predictive models. (nature.com)
  • Here we present a new algorithm that takes advantage of the unique properties of the transcriptional profiles of cancer samples to infer tumour cellularity as well as the different infiltrating normal cells, called ESTIMATE (Estimation of STromal and Immune cells in MAlignant Tumour tissues using Expression data). (nature.com)
  • We focus on stromal and immune cells that form the major non-tumour constituents of tumour samples and identify specific signatures related to the infiltration of stromal and immune cells in tumour tissues 1 . (nature.com)
  • As stem cells can be grown and transformed into specialized cells with characteristics consistent with cells of various tissues such as muscles or nerves, their use in medical therapies has been proposed. (patientslikeme.com)
  • The identification of such profiles in the horse will allow the comparison of putative MS cells isolated from different laboratories and different tissues. (dovepress.com)
  • In the ongoing efforts to develop therapies against chronic lymphocytic leukemia (CLL), stromal factors allowing malignant cells to escape spontaneous and chemotherapy-mediated apoptosis, giving way to relapses, have been abundantly investigated. (dovepress.com)
  • TUNEL assay was used to detect cell apoptosis after UV-irradiation. (molvis.org)
  • however, it was unable to induce apoptosis in leukemic cells co-cultured with stromal cells. (haematologica.org)
  • Similarly, small interfering ribonucleic acid (siRNA)-mediated RELA downregulation induced apoptosis of CLL cells cultured alone, but not in the presence of supportive stromal cells. (haematologica.org)
  • They observed a decrease in apoptosis in KG1a cells after co-cultured with modified HS27a cells under TNFɑ induction.The team further explained that these results indicate ITGAV plays a role in the co-culture system. (thermofisher.com)
  • The sMSCs were more sensitive to hydrogen peroxide and bleomycin treatment with respect to cell viability and apoptosis induction. (medsci.org)
  • ROCK1 is the key mediator of RhoA activity, and is a multifunctional member of the AGC (protein kinase A/G/C) kinase family that has also been implicated in the modulation of stress fiber assembly, cell contraction, apoptosis, migration, and invasion of multiple cancer cell types. (pubmedcentralcanada.ca)
  • Cellular therapy with mesenchymal stromal cells (MSC) has recently emerged as a promising strategy to regulate anti-donor immune responses, allowing immunosuppressive drug minimization and tolerance induction. (frontiersin.org)
  • We used this assay to correlate single-cell morphological data with overall immunosuppressive capacity in a cohort of MSC lines derived from different donors and manufacturing conditions. (pnas.org)
  • A major challenge in the development of consistently effective MSC-based immunosuppressive therapies is that MSC lines derived from different donors and manufacturing processes (i.e., cell expansion) can possess markedly dissimilar immunosuppressive function ( 3 , 5 , 6 ). (pnas.org)
  • Le Hir M, Hegyi I, Cueni-Loffing D, Loffing J, Kaissling B. Characterization of renal interstitial fibroblast-specific protein 1/S100A4-positive cells in healthy and inflamed rodent kidneys. (springer.com)
  • M-CSF, LIF), bone marrow stromal cells have been described to be involved in human haematopoiesis and inflammatory processes. (wikipedia.org)
  • According to Dr. Iacovitti, there has been little research into just how stem cell transplantation modifies inflammatory and immune effects as well as promotes regenerative effects, such as blood vessel growth. (nutritionreview.org)
  • Waterman R Waterman RS, Tomchuk SL et al (2010) A New Mesenchymal Stem Cell Paradigm: Polarization into a Pro-Inflammatory MSC-1 or an Immunosupressive VSC-2 Phenotipe, PLOS ONE, 5, 1-14. (scirp.org)
  • Importantly, 12 week-old Ins2-CCL21 TLOs contained FRC-like cells with higher contractility, regulatory, and anti-inflammatory properties and enhanced expression of β-cell autoantigens compared to non-transgenic NOD TLOs found in inflamed islets. (diabetesjournals.org)
  • however, emerging evidence suggests that the stromal compartment can shape antitumour immunity and responsiveness to immunotherapy. (nih.gov)
  • These methods take advantage of the differences in transcriptome properties of distinct cell types. (nature.com)
  • Methods: After adding EC to MSC in a direct-contact system, cell viability and morphology were investigated with the WST assay and immnostaining. (uib.no)
  • The authors emphasized that "it is essential to clarify the underlying mechanism before undertaking clinical trials with stem cell-based approaches for patients with cerebral stoke. (innovations-report.com)
  • Their results "may help fill in a piece of the 'missing link' between histologic findings and functional recovery in animal experiments and may be useful for further stem cell research. (innovations-report.com)
  • The timing of stem cell treatment was critical to the magnitude of the positive effects," said the study's lead author, Lorraine Iacovitti, Ph.D., professor, Department of Neuroscience at Jefferson Medical College of Thomas Jefferson University. (nutritionreview.org)
  • Expression of stem-cell related genes was measured using quantitative RT-PCR and immunohistochemistry. (aacrjournals.org)
  • Importantly, this treatment combination substantially down-regulated expression levels in 57 (68%) of the 84 stem-cell related genes, including 34 (67%) of the 51 genes up-regulated after the chemotherapy. (aacrjournals.org)
  • Human mesenchymal stem/stromal cells (hMSCs) are a potential cell source of stem cell therapy for many serious diseases and hMSC spheroids have emerged to replace single cell suspensions for cell therapy. (rsc.org)
  • We first demonstrated that poly( N -isopropylacrylamide- co -acrylic acid) (p(NIPAAm-AA)) was not toxic to hMSCs and the microgel-formed constructs facilitated formation of uniform stem cell spheroids. (rsc.org)
  • Megakaryocyte progenitors (burst forming unit-megakaryocyte, colony forming unit-megakaryocyte [CFU-MK], and megakaryoblasts) are immature cells that can expand to form megakaryocytic clusters, a process referred to as clonal expansion. (bloodjournal.org)
  • 6 The CFU-F assay is now widely used as a functional method to quantify stromal progenitors. (stemcell.com)
  • One important question is how the granule cell layer, and neuronal activity in general, instructs the pool of developing neural progenitors to increase or decrease the extent of neurogenesis, a process known as "excitation-neurogenesis coupling" ( Deisseroth and Malenka, 2005 ). (jneurosci.org)
  • This joint pairing will bring together basic and clinical scientists with interdisciplinary interests in stromal cell biology and disease treatment to discuss critical fundamental and translational advances. (keystonesymposia.org)
  • The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. (springer.com)
  • The present invention relates to an improved three-dimensional cell culture system in which cells are grown on a three-dimensional matrix while cycling the cultures between metabolically favorable and metabolically unfavorable (but noncytotoxic) conditions. (google.com)
  • Primary cultures of HLS cells were established in DMEM/F12 medium supplemented with either 10% fetal bovine serum (FBS) or 2% B27 supplement. (arvojournals.org)
  • HLS cultures containing cells of predominantly fibroblast lineage can be grown on fibroin-based materials, but this process is dependent upon additional ECM factors such as those provided by serum. (arvojournals.org)
  • Marrow samples from 89 patients with aplastic anemia (AA) were evaluated for their ability to grow stromal layers in standard long- term marrow cultures (LTMCs). (bloodjournal.org)
  • Rates and Deadlines listed below are for the 2020-Q5 "Stromal Cells in Immunity and Disease" conference only. (keystonesymposia.org)
  • The functional innate immunity (NK cells in particular) of these immunodeficient hosts, however, is a barrier to engraftment and maintenance of tumor architecture for many types of primary human cancers. (jax.org)
  • In this Review, we discuss the evidence that stromal determinants interact with leukocytes and influence antitumour immunity, with emphasis on the immunological attributes of stromal cells that may foster their protumorigenic function. (nih.gov)