• These effects are distinct from those of many motility and angiogenesis inhibitors that seem to act by a common mechanism involving the induction of actin stress fibers. (ubc.ca)
  • 19 , 20 Moreover, the overexpression of either dominant-negative or constitutively active RhoA prohibits induction of actin stress fibers and morphological restructuring, 19 demonstrating that shear modulation of short-term signaling events, such as temporal regulation of RhoA activity, is crucially linked to long-term outcomes of exposing endothelial cells to flow. (ahajournals.org)
  • The molecular mechanism by which FHL3 induced actin stress fiber disassembly was demonstrated by low speed actin co-sedimentation assays and electron microscopy. (nih.gov)
  • X-ray scattering and microscopy analysis shows that the synthetic fibers exhibit molecular and fibrillar alignment, as well as D-banding formation consistent with native collagen. (northeastern.edu)
  • This work is the first to show direct evidence that nano-carbon fillers may promote collagen molecular and fibril alignment during both self-assembly and the application of external stress. (northeastern.edu)
  • We develop a model of mechanical signal transmission through prestressed viscoelastic actin stress fibers that directly connect the cell surface to the nucleus. (biomedsearch.com)
  • Collagen and collagen/nano-carbon fibers which exhibit multi-scale structure similar to native tendon were formed by a gel-spinning approach. (northeastern.edu)
  • For the collagen composite fibers, the rod-like nature of carbon nanotubes is exploited to understand its potential along with other rigid nano-carbons to promote ordered assembly of biological materials. (northeastern.edu)
  • Beyond ordering, the nano-carbons used were also able to reinforce the collagen composite fibers. (northeastern.edu)
  • Contact guidance refers to a phenomenon for which the orientation of cells and stress fibers is influenced by geometrical patterns such as nano/microgrooves on substrates, or collagen fibers in gels and soft tissues. (wikipedia.org)
  • For those conditions, it has been demonstrated that the geometrical cues provided by collagen or scaffold fibers are able to influence the orientation of cells. (wikipedia.org)
  • Similarly, it has been demonstrated that the collagen fibers present in collagen gels and soft tissues can influence cell alignment, providing the most important stimulus in terms of cell orientation Recent research has highlighted the importance of cellular alignment for the mechanical properties and functionality of the prostheses developed using the principles of tissue engineering. (wikipedia.org)
  • Bovine aortic endothelial cells were subjected to laminar shear stress (10 dyne/cm 2 ) for up to 6 hours. (ahajournals.org)
  • Similarly, stress fibers failed to form in endothelial cells exposed to enhanced blood flow in caveolin-1 knockout mice. (ahajournals.org)
  • 1 Several, now classic, studies 2 - 5 illustrate that in vitro and in vivo, actin stress fibers orient parallel to the direction of flow and are prominent in endothelial cells subjected to high shear velocities. (ahajournals.org)
  • Dynamics of mechanical signal transmission through prestressed stress fibers. (biomedsearch.com)
  • The analysis considers both temporally stationary and oscillatory mechanical signals and accounts for cytosolic drag on the stress fibers. (biomedsearch.com)
  • To elucidate the physical parameters that govern mechanical signal transmission, we initially focus on the highly simplified case of a single stress fiber. (biomedsearch.com)
  • The results demonstrate that the dynamics of mechanical signal transmission depend on whether the applied force leads to transverse or axial motion of the stress fiber. (biomedsearch.com)
  • For transverse motion, mechanical signal transmission is dominated by prestress while fiber elasticity has a negligible effect. (biomedsearch.com)
  • Mechanical signal transmission is significantly delayed by stress fiber material viscosity, while cytosolic damping becomes important only for longer stress fibers. (biomedsearch.com)
  • For simple networks of stress fibers, mechanical signals are transmitted rapidly to the nucleus when the fibers are oriented largely orthogonal to the applied force, whereas the presence of fibers parallel to the applied force slows down mechanical signal transmission significantly. (biomedsearch.com)
  • By applying large deformations with a soft cantilever we were able to fully characterize the mechanical response of the fibers and evaluate their tension, extensibility, elastic and viscous properties. (epfl.ch)
  • The mechanical behaviors of steel fiber reinforced high-performance concrete with fly ash (SFRHPFAC) are studied in this paper through both static compression test and dynamic impact test. (hindawi.com)
  • Various types of fiber-reinforced reactive powder concrete matrix were tested for making more usable and accurate estimation equations for mechanical properties for ultra high strength concrete. (hindawi.com)
  • In addition to making contact with other cells, the contact-inhibited cells must also be forced to reduce its cell area under the mechanical stress and constraints imposed by surrounding cells. (wikipedia.org)
  • During post-processing or elongation of the fibers, the nano-carbons were capable of inducing higher levels of fibril alignment and packing as compared to control fibers. (northeastern.edu)
  • Our data suggests that parallel human selection for enhanced fiber growth in several geographically widely dispersed species of domesticated cotton resulted in similar and overlapping metabolic transformations of the manner in which cellular redox levels have become modulated. (biomedcentral.com)
  • The addition of steel fibers into concrete mix can significantly improve the engineering properties of concrete. (hindawi.com)
  • Studies have proved that the addition of steel fibers into concrete significantly improved many of the engineering properties of mortar and concrete, notably compressive and flexural strength, impact strength, and toughness. (hindawi.com)
  • From the test results, steel fibers significantly increase the ductility, strength and stiffness of ultra high strength matrix. (hindawi.com)
  • A new equation is proposed to anticipate the maximal shear and normal stresses at the interface goal to anticipate the failure mode due to the &bonding of the plate. (scribd.com)
  • Methods and Results- Hemodynamic shear stress influences the phenotype of the endothelium. (ahajournals.org)
  • From our own work, we found that a signaling network composed of β1 integrin and caveolin-1 develops in response to shear stress. (ahajournals.org)
  • Interfacial properties for steel fiber in mortar were derived from the measured fiber load versus displacement curves, based on a unified fiber debonding theory. (concrete.org)
  • To study the evolution of oxidative stress related genes in a single plant cell, we conducted comparative expression profiling analyses of the elongated seed trichomes ("fibers") of cotton ( Gossypium ), using a phylogenetic approach. (biomedcentral.com)
  • This difference in mechanism of action sets strongylophorine-26 apart as an experimental anticancer agent and indicates that pharmacologic inhibition of cell migration may be achieved by mechanisms not involving the stabilization of actin stress fibers. (ubc.ca)
  • In that case, the stress fiber assembly is isotropic, and these fibers can pull on their surroundings in an isotropic fashion, and consequently the resulting cell shape is isotropic (without a preferred alignment). (wikipedia.org)
  • The present study describes an improved fluorescent recovery after photobleaching (FRAP) technique, which has been successfully used to quantify actin dynamics within individual fibers. (nih.gov)
  • Cylindrical and cube specimens with three volume fractions of end-hooked steel fibers with volume fraction of 0.5%, 1.0%, and 1.5% (39.25, 78.50, and 117.75 kg/m 3 ) and aspect ratio of 64 are used. (hindawi.com)
  • Karahan and Atiş [ 1 ], while studying the properties of polypropylene fiber reinforced fly ash concrete, reported that adding a volume fraction of 0%, 0.05%, and 0.10% polypropylene fiber seemed to slightly increase or maintain the strength, but when 0.20% of polypropylene fiber was added, the strength reduced. (hindawi.com)
  • The composition is in particular suitable to alleviate abdominal discomfort, abdominal pain, abdominal cramps, and bowel movement disturbances;in as far as these conditions and symptoms are associated with stress. (google.com)
  • One application of composite materials in civil engineering is examined: the strengthening of a reinforced concrete beam in situ by externally-bonded fiber reinforced plastic (FRP). (scribd.com)
  • The results reveal that the failure mode of concrete considerably changes from brittle to ductile with the addition of steel fibers. (hindawi.com)
  • Preventing brittle failure of this type of matrix, steel fibers were used. (hindawi.com)
  • The subsequent fluorescent recovery over a 10-min imaging period was assessed from a series of intensity profiles, positioned along the length of the stress fibers and spanning the bleach region. (nih.gov)
  • Peak fiber and wall stress occurred in early systole (97±7 and 81±4 msec after the onset of ejection, respectively), before important contributions of Pb to central pressure. (ahajournals.org)