• All steps in the replication of a lagging strand have been coupled in a model system that catalyzes the formation of covalently closed, circular, double-stranded DNA molecules using single-stranded viral DNA as template. (neb.com)
  • Studies in mouse suggest that telomerase also participates in chromosomal repair, since de novo synthesis of telomere repeats may occur at double-stranded breaks. (nih.gov)
  • Unlike DNA, which mostly exists as double-stranded structures that are well defined and exhibit low structural and binding diversity, RNA is typically single-stranded and naturally assumes complex three-dimensional structures. (nature.com)
  • After the DNA library is sequenced on the Illumina flow cell, it is enzymatically double stranded by extension of a 5′ biotinylated primer. (nature.com)
  • The drug leads to single-stranded and double-stranded DNA breaks that arrest cellular proliferation in the late S or early G2 phase of cell cycle. (medscape.com)
  • The six subunit Origin Recognition Complex (ORC) together with Cdc6 and Cdt1, load the MCM replicative helicase as a double hexamer around double stranded DNA at origins. (crick.ac.uk)
  • MCM2-7 complexes unwind the double stranded DNA at the origins, recruit DNA polymerases and initiate DNA synthesis. (yeastgenome.org)
  • Polymerases must travel on a helical path during successive rounds of nucleotide incorporation to generate a helical, double-stranded DNA product. (mpg.de)
  • It is the largest (220 nm in diameter) and most complex herpesvirus, with a 235,000 double-stranded DNA genome. (medscape.com)
  • Cytomegalovirus (CMV) is a double-stranded DNA virus and is a member of the Herpesviridae family. (medscape.com)
  • Replicative DNA polymerases have evolved the ability to copy the genome with high processivity and fidelity. (pasteur.fr)
  • DNA polymerases (DNAPs) are the key enzymes of DNA replication and diverse DNA repair processes. (pasteur.fr)
  • Similar to other replicative DNA polymerases, the activity of PolD is strongly stimulated through its interaction with PCNA. (pasteur.fr)
  • Eukaryotic DNA polymerases? (flashcardmachine.com)
  • To ensure genome integrity, this sophisticated molecular machine must coordinate events over a broad range of time and length scales, from the breaking and reformation of chemical bonds within DNA polymerases to the large-scale structural rearrangements of chromosomes. (mpg.de)
  • Since the physical tethering of the polymerases within the replisome holds them in fixed orientations, the daughter strands must rotate to accommodate the path of synthesis. (mpg.de)
  • formed by continuously copying the parent strand 3' to 5' toward the replication fork. (flashcardmachine.com)
  • During replication, separation of the two daughter-strand templates by the helicase leads to overwinding and the formation of positive supercoils ahead of the replication fork. (mpg.de)
  • PCNA is required for the coordinated synthesis of both leading and lagging strands at the replication fork during DNA replication. (bvsalud.org)
  • Replication of the lagging strand of bacteriophage T7 DNA occurs in a discontinuous fashion that requires RNA-primed DNA synthesis, the removal of the RNA primers, the replacement of the ribonucleotides with deoxyribonucleotides, and the covalent joining of adjacent DNA fragments. (neb.com)
  • DNA Synthesis occurs during what phase of Mitosis? (flashcardmachine.com)
  • It is likely that this pathway utilized a flap endonuclease to process the single-stranded DNA that occurs during repair synthesis. (jefferson.edu)
  • The researchers mapped EdU-induced excision repair at high resolution and found that it occurs across the genome, and it apparently occurs again and again, since each new repair strand includes EdU and thus provokes the repair response anew. (newswise.com)
  • We demonstrated that DNA synthesis occurs discontinuously only on one arm of replication forks (the arm where the direction of synthesis is opposite to the direction of fork movement) through the repeated initiation, synthesis and joining of Okazaki fragments (transient nascent DNA chains of 40 to 300 nucleotides). (nih.gov)
  • Niacin, another B vitamin which occurs in two forms (nicotinic acid and nicotinamide), also contributes to the maintenance of DNA integrity due to several important roles. (cambridge.org)
  • Cisplatin inhibits DNA synthesis and, therefore, cell proliferation, by causing DNA cross-linking and denaturation of the double helix. (medscape.com)
  • RNA and use it to synthesis DNA by reverse transcriptase. (flashcardmachine.com)
  • mRNA was isolated, heat fragmented and the fragmented mRNA was then converted to single-stranded cDNA using SuperScript III reverse transcriptase and P7-t25-vn oligo-dT primer. (nih.gov)
  • Tetraribonucleotides (pppACCC or pppACCA), synthesized by the T7 gene 4 protein on single-stranded DNA, are used as primers by T7 DNA polymerase to yield RNA-terminated DNA fragments. (neb.com)
  • The removal of the RNA primers is catalyzed by the 5' to 3' hydrolytic activities of either Escherichia coli DNA polymerase I or the T7 gene 6 exonuclease. (neb.com)
  • Here we report on a simple and highly accessible approach to convert commercially available customized DNA microarrays of any complexity and density to RNA microarrays via a T7 RNA polymerase-mediated extension of photocrosslinked methyl RNA primers and subsequent degradation of the DNA templates. (nature.com)
  • The human mitochondrial transcription machinery generates the RNA primers needed for initiation of heavy strand DNA synthesis. (nih.gov)
  • Adds deoxyribonucleotides to 3'OH of RNA primers/end of growing DNA strand. (flashcardmachine.com)
  • Secondly, RT performs DNA-directed plus-strand DNA synthesis using the PPTs that have not been removed by RNase H as primers. (proteopedia.org)
  • The DNA is looped out to search for the nearest d(GATC) methylation site to the mismatch, which could be up to 1 kb away. (wikipedia.org)
  • Targeting DNA methylation with small molecules: what's next? (google.fr)
  • It is also able to detect single nucleotide polymorphisms, insertion-deletions or other sequence variations, in addition to being able to quantify DNA methylation and allele frequency. (news-medical.net)
  • The method of drug resistance in a new strain of bacteria can be determined by its genetic change compared to an ancestor, or epigenetic methylation of DNA in a cancer cell can be detected in order to determine the best course of treatment. (news-medical.net)
  • DNA methylation and histone modifications play a central role in the epigenetic regulation of gene expression and cell differentiation. (cipsm.de)
  • Recently, Np95 (also known as UHRF1 or ICBP90) has been found to interact with Dnmt1 and to bind hemimethylated DNA, indicating together with genetic studies a central role in the maintenance of DNA methylation. (cipsm.de)
  • Synthesis of lagging strands in a reconstituted system using purified proteins. (neb.com)
  • A combination of four bacteriophage proteins, gene 4 protein, Form II of T7 DNA polymerase, gene 6 exonuclease, and DNA ligase, can accomplish this overall reaction. (neb.com)
  • We find that this type of non-canonical fork convergence in fission yeast is prone to trigger deletions between repetitive DNA sequences via a mechanism we call Inter-Fork Strand Annealing (IFSA) that depends on the recombination proteins Rad52, Exo1 and Mus81, and is countered by the FANCM-related DNA helicase Fml1. (elifesciences.org)
  • The discipline of biophotonics deals with the interaction of light, or electromagnetic radiation, with living organisms and biologically active macromolecules: proteins (hemoglobin), nucleic acids (DNA and RNA), and metabolites (glucose and lactose). (nationalacademies.org)
  • Bailis JM and Forsburg SL (2004) MCM proteins: DNA damage, mutagenesis and repair. (yeastgenome.org)
  • Single strand binding proteins do what? (flashcardmachine.com)
  • As a class, students work through an example showing how DNA provides the "recipe" for making human body proteins. (teachengineering.org)
  • Once it was discovered that DNA contains the instructions for making cells and proteins, the way was open for many diverse engineering applications. (teachengineering.org)
  • Genomic DNA replication is carried out by so-called replicative DNAPs, which have evolved to copy the genome with high processivity and fidelity. (pasteur.fr)
  • Transcription from the light strand promoter is prematurely terminated at positions 300-282 in the mitochondrial genome, which coincide with the major RNA-DNA transition points in the D-loop of human mitochondria. (nih.gov)
  • The ability of human nuclear DNA to cause false positive low-abundance heteroplasmy calls varies across the mitochondrial genome. (utmb.edu)
  • Paclitaxel protein bound is a microtubular inhibitor (albumin-conjugated formulation) and a natural taxane that prevents depolymerization of cellular microtubules, which results in DNA, RNA, and protein synthesis inhibition. (medscape.com)
  • Durch die Beteiligung von PARP1 an der DNA- Reparatur ist dessen Inhibition in der Krebstherapie von großem Interesse. (uzh.ch)
  • Initiating the repair of DNA single strand breaks, inhibition of PARP1 is an emerging field in cancer treatment. (uzh.ch)
  • Germline pathogenic variants in the exonuclease domain of the replicative DNA polymerase Pol ε encoded by the POLE gene, predispose essentially to colorectal and endometrial tumors by inducing an ultramutator phenotype. (bvsalud.org)
  • Replication may be categorized into immediate early, delayed early, and late gene expression based on time of synthesis after infection. (medscape.com)
  • In order to begin repair, the mismatch repair machinery distinguishes the newly synthesised strand from the template (parental). (wikipedia.org)
  • In gram-negative bacteria, transient hemimethylation distinguishes the strands (the parental is methylated and daughter is not). (wikipedia.org)
  • Most DNA replication events from the heavy strand origin are prematurely terminated, forming a persistent RNA-DNA hybrid, which remains annealed to the parental DNA strand. (nih.gov)
  • Chromosome replication is performed by a large multisubunit assembly, known as the replisome, which couples the unpackaging of parental DNA with the synthesis of new daughter strands. (mpg.de)
  • We seek to define the operating principles that guide replisome function and understand how replisomes recognize and process obstacles encountered on parental chromosomes such as DNA lesions, unfavorable topological structures, and architectural factors. (mpg.de)
  • However, parental histones must also be transferred to the daughter-strands to ensure epigentic information is faithfully replicated. (mpg.de)
  • Affinity Plus DNA & RNA Oligonucleotides are custom, single-stranded, and duplexed sequences that contain 1−20 locked nucleic acid nucleotides. (idtdna.com)
  • Pyrosequencing is a method of DNA sequencing that detects light emitted during the sequential addition of nucleotides during the synthesis of a complementary strand of DNA. (news-medical.net)
  • One of the four types of nucleotides that make up DNA are added to the wells, which begin to be incorporated onto the single-strand DNA template by DNA polymerase at the 3' end, releasing pyrophosphate.ATP sulfurylase then converts pyrophosphate to adenosine triphosphate (ATP) in the presence of adenosine 5' phosphosulfate. (news-medical.net)
  • For example, if three cytosine nucleotides are added sequentially to the same fragment of DNA, the light emitted will be three times more intense than fragments of DNA with only one cytosine nucleotide added. (news-medical.net)
  • If no light is emitted upon the addition of cytosine, then the next complimentary base in the single-strand DNA template must be one of the other three nucleotides. (news-medical.net)
  • Loaded PCNA then directs the action of the MutLalpha endonuclease to the daughter strand in the presence of a mismatch and MutSalpha or MutSbeta. (wikipedia.org)
  • In Eukarya and Archaea, their processivity is greatly enhanced by binding to the proliferative cell nuclear antigen (PCNA) that encircles the DNA. (pasteur.fr)
  • By using an integrative approach which combines cryo-electron microscopy and X-ray crystallography, the Unit of Structural Dynamics of Macromolecules deciphered the recruitment and cooperativity mechanisms between DNA polymerase D and PCNA. (pasteur.fr)
  • In eukaryotes and archaea, PCNA stimulates processive DNA synthesis of both lagging and leading strands. (pasteur.fr)
  • In their work, the Unit of Structural Dynamics of Macromolecules presented the structure of the DNA-bound PolD-PCNA complex from P. abyssi at 3.77 Å using an integrative structural biology approach, combining cryo-EM, X-ray crystallography, protein-protein interaction measurements and activity assays. (pasteur.fr)
  • Motea, E.A. & Berdis, A.J. Terminal deoxynucleotidyl transferase: the story of a misguided DNA polymerase. (nature.com)
  • PolD is an archaeal replicative DNA polymerase, which is widely distributed among Archaea and has been shown to be essential for cell viability. (pasteur.fr)
  • As we speak, hundreds and maybe thousands of researchers use EdU to study DNA replication and cell proliferation in lab experiments without knowing that human cells detect it as DNA damage," Sancar said. (newswise.com)
  • DNA replication is the primary event that regulates cellular and viral proliferation. (nih.gov)
  • Bloom syndrome patients also demonstrate impairment in lymphocytic proliferation, deficient immunoglobulin synthesis, and lowered response to mitogen stimulation, resulting in impairment of both cellular and humoral immune responses. (medscape.com)
  • Figure 3: Sequence confirmation of 10-mer synthesis. (nature.com)
  • This triple-stranded structure is called the D-loop and encompasses the conserved sequence box II, a DNA element required for proper primer formation. (nih.gov)
  • Megamer Single-Stranded DNA Fragments are sequence-verified ssDNA fragments 201-2000 bases in length. (idtdna.com)
  • Structural and Molecular Basis for Coordination in a Viral DNA Packaging Motor. (utmb.edu)
  • Drugs that block DNA replication can arrest the spread of cancer cells and eliminate viral pathogens. (nih.gov)
  • In the past, our research focused on viral genomes as models for DNA replication in mammalian cell nuclei. (nih.gov)
  • Beaucage, S.L. & Caruthers, M.H. Deoxynucleoside phosphoramidites-A new class of key intermediates for deoxypolynucleotide synthesis. (nature.com)
  • Etoposide is a glycosidic derivative of podophyllotoxin that exerts a cytotoxic effect by stabilizing the normally transient covalent intermediates formed between the DNA substrate and topoisomerase II. (medscape.com)
  • Results from our lab, as well as from other labs, led to the identification of all of the various DNA replication intermediates in SV40 replication. (nih.gov)
  • Importance of disentanglement and entanglement during DNA replication and segregation: Comment on: "Disentangling DNA molecules" by Alexander Vologodskii. (utmb.edu)
  • Therefore, the overall goal of our work is to discover how DNA replication is regulated both in the large chromosomes of cells and in the "mini-chromosomes" of viruses and small extrachromosomal DNA molecules. (nih.gov)
  • An exonuclease trails the complex and digests the ss-DNA tail. (wikipedia.org)
  • The single-strand gap created by the exonuclease can then be repaired by DNA Polymerase III (assisted by single-strand-binding protein), which uses the other strand as a template, and finally sealed by DNA ligase. (wikipedia.org)
  • Bst 2.0 WarmStart DNA Polymerase contains 5´→3´ DNA polymerase activity and strong strand-displacement activity but lacks 5´→3´ exonuclease activity. (neb.com)
  • Any mutational event that disrupts the superhelical structure of DNA carries with it the potential to compromise the genetic stability of a cell. (wikipedia.org)
  • disrupts DNA function by covalently binding to DNA bases. (medscape.com)
  • Separation of the two single strands leads to the build-up of superhelical tension, which, if left unresolved, exerts extreme forces in the form of torque that disrupts critical enzymatic events on chromosomes. (mpg.de)
  • Eukaryotic DNA replication initiates at multiple origin sites along each chromosome and terminates when replication forks (RFs) from adjacent origins converge. (elifesciences.org)
  • DNA replication in eukaryotic cells initiates from a large number of chromosomal sites known as origins. (crick.ac.uk)
  • DNA mismatch repair (MMR) is a system for recognizing and repairing erroneous insertion, deletion, and mis-incorporation of bases that can arise during DNA replication and recombination, as well as repairing some forms of DNA damage. (wikipedia.org)
  • This so-called recombination-dependent replication (RDR) helps ensure that DNA is fully replicated prior to sister chromatid segregation, thereby avoiding mitotic catastrophes. (elifesciences.org)
  • This protein plays a pivotal role in DNA recombination and repair. (medscape.com)
  • Kosuri, S. & Church, G.M. Large-scale de novo DNA synthesis: technologies and applications. (nature.com)
  • Bst 2.0 WarmStart DNA Polymerase is an in silico designed homologue of Bacillus stearothermophilus DNA Polymerase I, Large Fragment ( Bst DNA Polymerase, Large Fragment) with a reversibly-bound aptamer, which inhibits polymerase activity at temperatures below 45°C. The aptamer rapidly releases the Bst 2.0 WarmStart DNA Polymerase above 45°C and therefore no special activation step is needed to activate the polymerase. (neb.com)
  • This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3' to 5' endonucleasic mode. (proteopedia.org)
  • Essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells, associates with the origins of DNA replication to form part of the pre-replicative complex. (yeastgenome.org)
  • Activation of the MCM complex at origins by cyclin-dependent kinases and the CDC7 protein kinase (P06243) leads to initiation of DNA synthesis. (yeastgenome.org)
  • PARP enzymes are involved in normal cellular function (eg, DNA transcription and repair). (medscape.com)
  • Regulation of transcription factors via natural decoys in genomic DNA. (utmb.edu)
  • 2) What are the requirements for DNA replication and transcription at the beginning of mammalian development? (nih.gov)
  • In eukaryotic organisms, nucleosomes function as the most basic unit of chromosome organization directly binding and assembling on DNA to modulate DNA topology, drive compaction, and regulate transcription. (mpg.de)
  • While the double-helical structure of duplex DNA is advantageous for the storage and maintenance of genetic information, it poses major challenges during essential cellular processes, such as transcription and replication, when the information-rich DNA bases must be accessed. (mpg.de)
  • The requirement for DNA synthesis to fill the gap between adjacent DNA fragments can be fulfilled by Form II of T7 DNA polymerase but not by Form I. DNA synthesis catalyzed by Form II of T7 DNA polymerase eliminates gaps to create a substrate for DNA ligase whereas strand displacement synthesis catalyzed by Form I creates an aberrant structure that cannot be joined. (neb.com)
  • The DNA that is to be sequenced is broken up into fragments of around 100 base pairs of single-strand DNA. (news-medical.net)
  • The DNA fragments are incubated with DNA polymerase, ATP sulfurylase, and apyrase enzymes, and adenosine 5' phosphosulfate and luciferin substrates. (news-medical.net)
  • RNase H probably can proceed both in a polymerase-dependent (RNA cut into small fragments by the same RT performing DNA synthesis) and a polymerase-independent mode (cleavage of remaining RNA fragments by free RTs). (proteopedia.org)
  • Problems that arise during DNA replication can drive genomic alterations that are instrumental in the development of cancers and many human genetic disorders. (elifesciences.org)
  • Noteworthy, was our demonstration that although termination of DNA replication did not require specific DNA sequences, some DNA sequences did promote pausing of DNA replication forks in vivo (and DNA polymerase in vitro), and some sequences, such as thosewithin the termination region for SV40 DNA replication, did promote formation of catenated intertwines during separation of sibling chromosomes. (nih.gov)
  • Either the host or phage DNA ligase can effect the final covalent joining. (neb.com)
  • It is suspected that, in eukaryotes, newly synthesized lagging-strand DNA transiently contains nicks (before being sealed by DNA ligase) and provides a signal that directs mismatch proofreading systems to the appropriate strand. (wikipedia.org)
  • Oligo pools are individually synthesized single-stranded DNA sequences that range from 40 to 350 bases. (idtdna.com)
  • BML mutations thus result in defects in DNA repair and genomic instability in the somatic cells, predisposing the patients to cancer development. (medscape.com)
  • Oligonucleotides are almost exclusively synthesized using the nucleoside phosphoramidite method, even though it is limited to the direct synthesis of ∼ 200 mers and produces hazardous waste. (nature.com)
  • 16, 17 ) for B -DNA oligonucleotides. (lu.se)
  • Mismatch repair is strand-specific. (wikipedia.org)
  • The fact that the damage detection and repair systems are as complex as the replication machinery itself highlights the importance evolution has attached to DNA fidelity. (wikipedia.org)
  • Examples of mismatched bases include a G/T or A/C pairing (see DNA repair). (wikipedia.org)
  • Single-stranded DNA oligos specifically built for your homology‑directed repair (HDR) experiments. (idtdna.com)
  • DNA repair system (4 steps! (flashcardmachine.com)
  • This was followed by second-strand cDNA synthesis and end-repair using T4 DNA polymerase, Klenow DNA polymerase and T4 polynucleotide kinase. (nih.gov)
  • The investigations described in this thesis report two novel findings: (1) the identification of a new class of adenine specific single-stranded DNA glycosylases and (2) the first description of DNA repair activities in Plasmodium falciparum, the protozoan parasite that causes human malaria. (jefferson.edu)
  • The investigation reported in Chapter Three characterized the repair of uracil mismatches and apurinic/apyrimidinic (AP) sites in DNA by a total cell lysate from P. falciparum. (jefferson.edu)
  • P. falciparum appeared to repair the nicked strand exclusively by a long-patch BER pathway. (jefferson.edu)
  • Newswise - CHAPEL HILL, NC - Scientists at the UNC School of Medicine have made the surprising discovery that a molecule called EdU, which is commonly used in laboratory experiments to label DNA, is in fact recognized by human cells as DNA damage, triggering a runaway process of DNA repair that is eventually fatal to affected cells, including cancer cells. (newswise.com)
  • Sancar, who won the 2015 Nobel Prize for Chemistry for his seminal work on DNA repair, is one such scientist. (newswise.com)
  • When his lab began using EdU, his team unexpectedly observed that EdU-labeled DNA triggered a DNA repair response even when it wasn't exposed to DNA-damaging agents, such as ultraviolet light. (newswise.com)
  • Following up on the strange observation, the team discovered that EdU, for reasons that are still unclear, alters DNA in a way that provokes a repair response called nucleotide excision repair. (newswise.com)
  • That discovery was interesting in its own right, Sancar said, because it suggested that researchers using EdU to label DNA need to take into account its triggering of runaway excision repair. (newswise.com)
  • Nuclear antigen with a role in DNA synthesis, DNA repair, and cell cycle progression. (bvsalud.org)
  • Both diseases involve the BRAFT and FANCM complexes, which are important in DNA repair. (medscape.com)
  • Additionally, it was found that the removal of adenine by a single-strand DNA glycosylase activity was the first step in the cleavage of DNA. (jefferson.edu)
  • Irinotecan and its active metabolite SN-38 bind reversibly to the topoisomerase-1 DNA complex and prevent re-ligation of the single-strand breaks, leading to exposure time-dependent double-strand DNA damage and cell death. (medscape.com)
  • It can also cause DNA strand breakage, because of its effects on topoisomerase II. (medscape.com)
  • Both the native and recombinant forms of gelonin were found to cause the degradation of single stranded DNA. (jefferson.edu)
  • Caruthers, M.H. A brief review of DNA and RNA chemical synthesis. (nature.com)
  • Caruthers, M.H. The chemical synthesis of DNA/RNA: our gift to science. (nature.com)
  • EdU (5-ethynyl-2'-deoxyuridine) is essentially a popular scientific tool first synthesized in 2008 as an analog, or chemical mimic, of the DNA building block thymidine - which represents the letter "T" in the DNA code of adenine (A), cytosine (C), guanine (G) and thymine (T). Scientists add EdU to cells in lab experiments to replace the thymidine in DNA. (newswise.com)
  • Blow JJ and Dutta A (2005) Preventing re-replication of chromosomal DNA. (yeastgenome.org)
  • A few or up to thousands of base pairs of the newly synthesized DNA strand can be removed. (wikipedia.org)
  • When bound, the MutS2 dimer bends the DNA helix and shields approximately 20 base pairs. (wikipedia.org)
  • It blocks DNA and RNA synthesis by inserting between adjacent base pairs and binding to the sugar-phosphate backbone of DNA, inhibiting DNA polymerase. (medscape.com)
  • Hold the DNA in single strand conformation. (flashcardmachine.com)
  • results from their study show the importance of the RAD51 nucleoprotein filament conformation for stimulating DNA pairing by BLM . (medscape.com)
  • The drug binds to nucleic acids presumably by specific intercalation of the anthracycline nucleus with the DNA double helix. (medscape.com)
  • We are using this to understand the mechanism of MCM helicase loading and activation, how the replisome is assembled and regulated by protein kinases and how the DNA replication machinery interfaces with other aspects of chromosome biology. (crick.ac.uk)
  • The portion of the RNA-DNA heteroduplex is digested by the RNase H, resulting in a ssDNA product attached to the tRNA primer. (proteopedia.org)
  • RT uses the 3' end of this newly synthesized short ssDNA to perform the RNA-dependent minus-strand DNA synthesis of the whole template. (proteopedia.org)
  • High-throughput sequencing-RNA affinity profiling (HiTS-RAP) 22 is conceptually similar to RNA-MaP, but uses the E. coli replication terminator protein Tus to stall the RNA polymerase after transcribing the variable section of the DNA library. (nature.com)
  • Stereospecific Effects of Oxygen-to-Sulfur Substitution in DNA Phosphate on Ion Pair Dynamics and Protein-DNA Affinity. (utmb.edu)
  • As a post-doctoral fellow with Paul Berg, I developed a subcellular system that allowed simian virus 40 (SV40) DNA to continue replication in vitro. (nih.gov)
  • We were among the first to develop and exploit sub-cellular systems that allowed SV40 and polyomavirus to complete DNA replication in vitro. (nih.gov)
  • Because the mitochondrial DNA of P. falciparum appeared to be the target of gelonin's cytotoxic activity and contained single-stranded regions, we investigated the activity of gelonin on single stranded DNA. (jefferson.edu)
  • this recommendation does not preclude the preparation of DNA samples for subsequent archiving if the material is considered potentially useful for future studies. (who.int)
  • Here, we investigated a radioiodinated PARP inhibitor, [ 125 I]KX1, and show drug target specific DNA damage and subsequent killing of BRCA1 and non-BRCA mutant ovarian cancer cells at sub-pharmacological concentrations several orders of magnitude lower than traditional PARP inhibitors. (osti.gov)
  • The investigation showed that the lysate was capable of removing uracil from DNA, generating AP sites. (jefferson.edu)
  • Based on our findings, we propose a model for primer formation at the origin of heavy strand DNA replication. (nih.gov)
  • removes the RNA primer from the lagging strand. (flashcardmachine.com)
  • the 30-hydroxyl end of this RNA primer then serves as the site for attachment of the initial deoxyribonucleotide by a DNA polymerase. (forextrading-madeeasy.com)
  • RT uses the 3' end of the tRNA primer to perform a short round of RNA-dependent minus-strand DNA synthesis. (proteopedia.org)