We examined the effect of carrier material and storage conditions on the populations of two industry standard rhizobia, Bradyrhizobium japonicum USDA 110 for soybean ( Glycine max ) and Rhizobium tropici CIAT 899 for common bean ( Phaseolus vulgaris ) over 165 days, using the drop plate method on Congo Red Yeast Extract Mannitol Agar. (n2africa.org)
LOCUS DH986125 546 bp DNA linear GSS 22-AUG-2012 DEFINITION Bradyrhizobium japonicum USDA 6 DNA, BAC clone: Be061b01E11, reverse end. (nig.ac.jp)
Rhizobium17
Antimicrobial Activity of Rhizobium Japonicum and Bradyrhizobium Japonicum on Different Plant Pathogenic Fungal Strains. (bbrc.in)
Nandi R. G, Bara J. K, Shrivastava P. Antimicrobial Activity of Rhizobium Japonicum and Bradyrhizobium Japonicum on Different Plant Pathogenic Fungal Strains. (bbrc.in)
Normally, commercial cultivars of the soybean Glycine max are nodulated only by slow-growing Rhizobium japonicum (Keyser et al . (bbrc.in)
Peking) Rhizobium japonicum 191 is a member of a salt-tolerant group of R. japonicum recently described by (Keyser et al . (bbrc.in)
1979. Colorimetric enzyme-linked immunosorbent assay for the identification of strains of Rhizobium in culture and in the nodules of lentils. (hawaii.edu)
1981. Growth of Rhizobium strains at low concentrations of phosphate. (hawaii.edu)
1985. Predominance of fast-growing Rhizobium japonicum in a soybean field in the People's Republic of China. (hawaii.edu)
1987. Intra- and interspecific competition in Rhizobium fredii and Bradyrhizobium japonicum as indigenous and introduced organisms. (hawaii.edu)
Enhanced symbiotic performance and productivity of drought stressed common bean by inoculation with locally isolated, salt-tolerant strains of Rhizobium . (bashanfoundation.org)
Rhizobium, Bradyrhizobium, Mesorhizobium, Sinorhizobium and Azorhizobium-known as Rhizobia-are Gram-negative, nitrogen-fixing bacteria of agronomic importance because they perform nitrogen-fixing symbiosis with leguminous plants. (proteinlounge.com)
However, effectiveness in nitrogen fixation depends on the genotype of legumes and requires host specific Rhizobium strain for effective nodulation to boost the productivity of legumes. (ujecology.com)
Rhizobium strains undertaken for evaluation behave better in ecologically competent and symbiotically effective in nodulation for increased yield and thus recommended for the study area and similar agro-ecologies. (ujecology.com)
However, the fixation of nitrogen depends on the genotype of legume, Rhizobium strain, and the interactions of these with the bio-physical environment and symbiosis nodulation of Rhizobium (Giller, et al. (ujecology.com)
To have a successful establishment, inoculants strain must be able to survive in soil environment because the better survival rate and soil persistence of Rhizobium enhanced the possibility of effective nodulation and nitrogen fixation (Knezevic-vukcevic, 2011). (ujecology.com)
If not, poorly efficient Rhizobium strains may outcompete and gain an advantage over effective Rhizobium strains used for inoculation (Fujita et al. (ujecology.com)
2007). As a result inoculation with host-specific effective Rhizobium strains species is required for effective nodulation and nitrogen fixation (Goss, et al. (ujecology.com)
Rhizobium tropici CIAT899, prepared with filter mud carrier, achieved a shelf-life of 135 days and B. japonicum USDA110 contained over 10 9 cells g -1 for 105 days. (n2africa.org)
Microbiome data obtained from soil using 16S rRNA primers demonstrated that the diversity of bacteria belonging to the genus Bradyrhizobium increased in soybean-cropped soils compared to bulk soil regardless of inoculation status. (stir.ac.uk)
Soybean has the ability to interact with the bacteria Bradyrhizobium japonicum , the outcome of this interaction is a nitrogen fixing nodule that is capable of providing the plants nitrogen needs. (manitobapulse.ca)
Nitragine KM biological fertiliser is a free-running soy inoculant based on the strain of efficient legume bacteria Bradyrhizobium japonicum (soy symbiont). (ntc-bio.com)
A specific feature of Bradyrhizobium japonicum is ability to intrude root fibrilla of soy roots and cause formation of root nodules with bacteria. (ntc-bio.com)
The effectiveness of the microbial-plant system of soybean cultivar Merlin and strains of nodule bacteria Bradyrhizobium japonicum , under optimal conditions, increased the crop yield to 4.43 t/ha, which is 2.11 t/ha higher compared to the control and 1.01 t/ha higher relative to the experimental option with carbamide fertilizer. (bio-conferences.org)
Genomic2
Expression constructs for nopT1 (annotated as blr2140) Baf-A1 mw and nopT2 (annotated as blr2058) were made by cloning the full-length nopT1 and nopT2 PCR amplicons derived from B. japonicum genomic DNA template. (c-kitinhibitors.com)
Objective 2 had two parts: a) to screen a large collection of Bap strains for pectin degradation and utilization as a sole carbon source, b) to conduct a comparative genomic analysis that includes Bap strains that lack the capacity for pectin utilization. (auburn.edu)
Nodulation6
Alice: Transcriptional analysis of genes involved in nodulation in soybean roots inoculated with Bradyrhizobium japonicum strain CPAC 15. (embrapa.br)
strains for nodulation of grain and fodder legumes. (hawaii.edu)
Symbiotic performance of nodulation is highly governed by the abundance of effective rhizobia strain and its competitiveness (Laguerre et al. (ujecology.com)
Legume nodulation involves several complex steps and, although studied for many decades, much remains to be understood.ResultsThis research aimed at analyzing the global expression of genes in soybean roots of a Brazilian cultivar (Conquista) inoculated with Bradyrhizobium japonicum CPAC 15, a strain broadly used in commercial inoculants in Brazil. (scite.ai)
Therefore a field experiment was conducted at Jimma Agricultural Research Center (JARC) to determine appropriate rate of Bradyrhizobium japonicum strains on nodulation and seed yield of soybean in south western Ethiopia during 2017/18 main cropping season. (ijras.org)
The objective of this work was to evaluate, by RT-qPCR, the expression of the nodC and nodW nodulation genes and of the nopP gene of the CPAC 15 strain, which probably play a role in the infection of soybean roots. (scielo.org)
Nodule5
The nodule dry weight, shoot dry weight and acetylene reduction activity of the plant inoculated with Bradyrhizobium elkanii LauBG38 were significantly higher in ARA per plant, nodule and shoot dry weights than the other tested isolates in both Yezin -4 and Yezin-7 black gram varieties. (scirp.org)
Recently, peat-based root nodule bacterial inoculants containing TAL strains are using as biofertilizer in seven legumes distributed by Ministry of Agriculture and Irrigation, Myanmar [9]. (scirp.org)
1990. Influence of elevation and applied nitrogen on rhizosphere colonization and competition for nodule occupancy by different rhizobial strains on field-grown soybean and common bean. (hawaii.edu)
2014). Although soil may harbor certain ineffective nodule forming native rhizobia, however effective nodule formation largely depends upon the competitiveness of inoculants strain (Laguerre et al. (ujecology.com)
The sequence analysis confirmed that all isolates were categorized and identified as the genus Bradyrhizobium and they were conspecific with B . elkanii , B . sp. (scirp.org)
We expect that Myanmar Bradyrhizobium elkanii LauBG38 will be able to use as Biofertilizer for black gram cultivars. (scirp.org)
They did not cluster together in topologies based on the 16S rRNA or nodA gene sequences, but were scattered among other bradyrhizobia belonging to either the Bradyrhizobium japonicum or the Bradyrhizobium elkanii lineages. (hal.science)
Inoculants2
Results: This research aimed at analyzing the global expression of genes in soybean roots of a Brazilian cultivar (Conquista) inoculated with Bradyrhizobium japonicum CPAC 15, a strain broadly used in commercial inoculants in Brazil. (embrapa.br)
In 2017, three commercial inoculants containing elite Bradyrhizobium strains significantly increased plant biomass in plot trials with a soybean 000 maturity group variety (ES Comandor). (stir.ac.uk)
Rhizobia3
Rhizobia were isolated from the nodules and identified as the original inoculant species, B. diazoefficiens and B. japonicum. (stir.ac.uk)
2016 also confirmed the host-specific rhizobia strains of common bean and soybean adapted better to the local soil and environmental condition. (ujecology.com)
2001). Inoculation affects the microbial community by increasing desired rhizobia strain population in the rhizosphere (Siczek and Lipiec, 2016). (ujecology.com)
Nitrogen fixation4
Evaluation of the effectiveness of Myanmar Bradyrhizobim strains isolated from soil samples of major black gram growing areas of Myanmar for plant growth and nitrogen fixation w as studied in pot experiments with completely randomized design and three replicates. (scirp.org)
Bradyrhizobium japonicum has been used since 1957 in molecular genetics, physiology, and ecology due to its excellent ability in symbiotic nitrogen fixation. (proteinlounge.com)
2002). In this scenario, inoculating faba bean with effective and appropriate rhizobial strain is crucial to improve symbiotic nitrogen fixation and to boost its productivity (McKenzi et al. (ujecology.com)
Rhizobial strains have contributed to enhance nitrogen fixation thereby soil fertility and yield of legumes crops when they are inoculated to seeds with sufficient quantity. (ijras.org)
Inoculum2
Nitrogen fixing B. japonicum strains that interact with soybean are not native to Manitoba, and if soybean is planted, it is strongly recommended that the seeds are treated with a B. japonicum inoculum either on seed or in furrow. (manitobapulse.ca)
The current study was conducted in two sites (Logboayo and Soubré) in the south-west of Côte d'Ivoire, to evaluate the effect of IRAT-FA3 Bradyrhizobium japonicum strain inoculum on the agronomic performance of five varieties of soybean named Doko, Canarana, V3_2013, V6_2013 and IT_235. (academicjournals.org)
Genus2
Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain SW112 T was related to members of the genus Belliella , showing the highest similarity with Belliella aquatica TS-T86 T and Belliella baltica DSM 15883 T (96.5 % and 96.4 % sequence similarity, respectively). (microbiologyresearch.org)
On the basis of phylogenetic inference and phenotypic characteristics, it is proposed that strain SW112 T represents a novel species of the genus Belliella , for which the name Belliella marina sp. (microbiologyresearch.org)
Genes6
plantarum strains with gyrB primers (UP-1 & UP-2r), and b) to identify exuT and uxuB genes with exuT and uxuB primers in the Bap strains. (auburn.edu)
The hypotheses for objective 1 were i) gyrB primers (UP-1 & UP-2r) will identify Bap strains, and ii) exuT and uxuB primers will identify exuT and uxuB genes in Bap strains for utilization of sugars derived from pectin. (auburn.edu)
The Bap strains were then screened for the presence of the exuT and uxuB genes. (auburn.edu)
The hypotheses for objective 2 were a) pectin degradation capacity of Bap strains is linked to utilization of D-Glucuronate of D-galacturonate as an energy source, and b) whole genome sequence analysis will reveal all the pectin-associated and defective genes by RAST and CLC genomics software. (auburn.edu)
Os resultados obtidos confirmam a funcionalidade dos três genes na estirpe CPAC 15, com ênfase para o nopP, cuja funcionalidade em Bradyrhizobium japonicum foi descrita pela primeira vez. (scielo.org)
The results confirm the functionality of the three genes in the CPAC 15 strain, with an emphasis on the nopP, whose functionality in Bradyrhizobium japonicum was described for the first time. (scielo.org)
Species2
Several Bradyrhizobium species are able to induce effective nodules in black gram cultivars. (scirp.org)
Of 79 Bacillus species strains screened via gyrB universal primers, 59 were confirmed as Bacillus amyloliquefaciens subsp. (auburn.edu)
Glycine1
Genetic diversity of indigenous Bradyrhizobium nodulating promiscuous soybean [Glycine max (L) Merr. (wits.ac.za)
Based on their host-range, their nitrogen-fixing ability and strain competition experiments, bacterial strains isolated from Parasponia were thought to constitute a separate lineage that would account for their exceptional host affinity. (hal.science)
Amyloliquefaciens2
Strains of Bacillus amyloliquefaciens subsp. (auburn.edu)
None of the strains was found to cluster together with Bacillus amyloliquefaciens subsp. (auburn.edu)
The potential for survival of the Bradyrhizobium inoculant strains in soils was also demonstrated through the detection of the inoculant strain B. diazoefficiens SEMIA 5080 at relatively high populations (104 g-1 dry soil) using a qRT-PCR method with SNB-specific nodZ primers. (stir.ac.uk)
Indigenous1
Soe, K.M., Htwe, A.Z., Moe, K., Abiko, T. and Yamakawa, T. (2020) Phylogenetic Diversity and Evaluation the Effectiveness of Indigenous Bradyrhizobium Strains for Myanmar Black Gram ( Vigna mungo L. Hepper) Cultivars. (scirp.org)
Seeds1
Seeds will be inoculated with Bradyrhizobium japonicum (strain WB74) at planting by use of liquid inoculation, applied in the plant furrow. (opot.co.za)
Bacterium1
A Gram-stain-negative, rod-shaped bacterium, strain SW112 T , was isolated from a seawater sample collected from the Indian Ocean. (microbiologyresearch.org)
Soil3
This is the first report describing Bradyrhizobium strains that were isolated from soil samples of major black gram growing areas in Myanmar. (scirp.org)
An important aspect that is not being addressed in this study is the affect that these cropping rotations have on the microbial populations that affect soil health as well as the population of B. japonicum . (manitobapulse.ca)
Experiments were designed to evaluate the potential effects of pectin degradation and utilization by Bap strains on root colonization and plant growth-promotion using pectin amendment to soil. (auburn.edu)
Colonization1
The overall objective of this study was to determine the potential role of pectin degradation and utilization in root colonization and plant growth-promotion of soybean by specific PGPR strains. (auburn.edu)
Sequence1
The whole genome sequence was determined for Bap strains AP194 and AP214 using an Illumina MiSeq sequencer based on in vitro test results. (auburn.edu)
Cultivars1
However, the effectiveness of symbiotic N 2 fixation may be an important factor to take into consideration through successful management of symbiosis between black gram cultivars and effective bradyrhizobial strains. (scirp.org)
Growth1
Bradyrhizobium japonicum synthesizes periplasmic cyclic beta-(1,3),beta-(1,6)-D-glucans during growth under hypoosmotic conditions. (unl.edu)
Genotypes1
The genotypes of the majority of the recombinant strains analyzed were similar to only one of the parental strains and the low frequency of polymorphic RAPD bands suggested that the nuclear fusions may not occur into the heterokaryon. (scielo.org)
Utilization2
strain differences in phosphate storage and utilization. (hawaii.edu)
Pectin degradation and utilization activity of 59 Bap strains were tested in vitro on Pectate Agar (PA) and Tris-Spizizen Salts (TSS) medium. (auburn.edu)
Populations1
The ability to use this experimental plot to ask these questions is an opportunity to address fundamental questions about B. japonicum persistence and provide insight to how microbial populations are correlated with crop productivity. (manitobapulse.ca)
Growing region1
strains were found in Salingyi Sagaing Region black gram growing region. (scirp.org)
Plant1
It is possible that Bap strains obtain some carbon via production of extracellular pectate lyase enzymes that degrade pectin in plant root cells. (auburn.edu)
Found1
Out of 59 Bap strains, 57 were found to contain exuT and 52 contained uxuB. (auburn.edu)