- Biological control of crown gall was achieved in a field experiment in which peach 'Missour' seedlings were dipped first in a suspension of A radiobacter K84 and subsequently in a suspension of a local agrocin-sensitive strain. (agronomy-journal.org)
- Résumé - - Fréquence du crown gall dans les pépinières fruitières d'Algérie, caractérisation des souches d'Agrobacterium tumefaciens et contrôle biologique par la souche antagoniste Agrobacterium radiobacter K84. (agronomy-journal.org)
- Agrobacterium radiobacter (more commonly known as Agrobacterium tumefaciens) is the causal agent of crown gall disease (the formation of tumours) in over 140 species of eudicots. (wikipedia.org)
- agrtu-PCAD Rhizobium radiobacter (Agrobacterium tumefaciens) (Agrobacterium radiobacter), Agrobacterium sp. (inrae.fr)
- thus, suggesting that biovar 2 strains are most likely to harbor a nopaline/agrocinopine A-type Ti-plasmid. (agronomy-journal.org)
- Agrobacterium tumefaciens infects the plant through its Ti plasmid. (wikipedia.org)
- The strain carried the pCAMBIA2300s plasmid, a double vector with the marker gene neomycin phosphotransferase II (npt II). (researchsquare.com)
- Our GV3101 strain harbors the C58 chromosomal backbone containing rifampicin resistance and the Ti plasmid pmp90 (pTiC58DT-DNA) harboring the gentamicin resistance. (goldbio.com)
- A functional T-DNA binary system can be built using our GV3101 strains as the T-DNA region has been deleted in the Ti plasmid and instead has a binary vector containing the missing T-region. (goldbio.com)
- Currently, there are several reports on standardisation of protocol for the Agrobacterium-mediated transformation. (wikipedia.org)
- Agrobacterium tumefaciens strain, LBA4404 with pCAMBIA1303 vector and construct pCAMBIA1303 TPS1 were individually used for transformation. (ias.ac.in)
- This study aims to speed up the process of Agrobacterium-mediated transformation of cucumber. (researchsquare.com)
- Thus, an effective protocol for Agrobacterium tumefaciens-mediated genetic transformation of cucumber was optimized. (researchsquare.com)
- Direct shoot organogenesis and Agrobacterium tumefaciens mediated transformation of Solanum trilobatum L. (tubitak.gov.tr)
- An efficient protocol for direct shoot organogenesis and Agrobacterium tumefaciens mediated transformation using in vitro derived leaf explants of S. Trilobatum was established for the first time. (tubitak.gov.tr)
- The Agrobacterium strain EHA 105 harboring the binary vector pCAMBIA 1301, which contained hygromycin phosphotransferase (hpt) as a selectable marker gene and β-glucuronidase (gus) as a reporter gene, was used for the transformation. (tubitak.gov.tr)
- GoldBio's GV3101 Agrobacterium chemically competent cells allow you to obtain high transformation efficiency in applications such as gDNA or cDNA library construction. (goldbio.com)
- Our system is often used for Agrobacterium -mediated transformation in mono and dicotyledonous species such as Arabidopsis thaliana , tobacco, potato, soybeans and corn. (goldbio.com)
- Some strains may chemotactically move towards chemical exudates from plants, such as acetosyringone and sugars, which indicate the presence of a wound in the plant through which the bacteria may enter. (wikipedia.org)
- β glucosidases from the bacteria Agrobacterium tumefaciens (Cbg1), Butyrivibrio fibrisolvens (bglA), Clostridium thermocellum (bglB), Escherichia coli (bglX), Erwinia chrysanthemi (bgxA) and Ruminococcus albus. (expasy.org)
- Possible plant compounds that initiate Agrobacterium to infect plant cells: Acetosyringone and other phenolic compounds alpha-Hydroxyacetosyringone Catechol Ferulic acid Gallic acid p-Hydroxybenzoic acid Protocatechuic acid Pyrogallic acid Resorcylic acid Sinapinic acid Syringic acid Vanillin To transfer the T-DNA into the plant cell, A. tumefaciens uses a type IV secretion mechanism, involving the production of a T-pilus. (wikipedia.org)
- Unlike the nitrogen-fixing symbionts, tumor-producing Agrobacterium species are pathogenic and do not benefit the plant. (wikipedia.org)
- Little is known about the pathogenic potential of these strains. (frontiersin.org)
- We conclude that the TA-iaaM gene of 'large TA' strains is specifically required to counteract the tumour growth inhibiting activity of the T-ipt gene. (nih.gov)
- Agrobacterium tumefaciens strain LBA 4404 carrying a binary vector pCAMBIA1301 harboring the CaMV35S promoter driven modified GUS gene was used. (ajol.info)
- Cloning and sequencing of an Agrobacterium tumefaciens beta-glucosidase gene involved in modifying a vir-inducing plant signal molecule. (expasy.org)
- Xintaimici', a very popular and typical north China-type cucumber variety, was transformed with Agrobacterium GV3101. (researchsquare.com)
- GV3101 Agrobacterium chemically competent cells should be stored at -80°C, pCAMBIA1391z Control DNA should be stored at -20°C and recovery medium should be stored at 4°C immediately upon arrival. (goldbio.com)
- conjugal transfer protein [Agrobacterium tumefaciens str. (sjtu.edu.cn)
- Agrobacterium tumefaciens is an Alphaproteobacterium of the family Rhizobiaceae, which includes the nitrogen-fixing legume symbionts. (wikipedia.org)
- However, the microbial metabolic pathway of the fungicide and the genetic systems driving its degradation by strain P3 remain unknown. (nature.com)
- In this study, DNA sequence data were used to characterize 290 Fusarium strains isolated during a survey of root-colonizing endophytic fungi of agricultural and nonagricultural plants in northern Kazakhstan. (researchgate.net)
- Five new 3- O -alkyl-4a,10a-dihydrofusarubins ( 2 - 6 ) were isolated from the culture filtrate of a strain of Fusarium sp. (go.jp)
- They isolated a Pseudomonas azelaica strain HPB1 which was able to degrade OPP through the production of 2,3-dihydroxybiphenyl. (nature.com)
- Composition of Lipopolysaccharide from Strains of Pseudomonas syringae pv. (microbiologyresearch.org)
- Summary: Lipopolysaccharide (LPS) from strains of Pseudomonas syringae pv. (microbiologyresearch.org)
- Les espèces les plus touchées sont le pêcher (5,39%), l'amandier (3,43%), le cerisier (1,50%), le pommier (1,47%) et l'olivier (1,30%) (Tableau I). Au sein d'une même espèce, la proportion de plants atteints de crown gall varie en fonction du porte-greffe utilisé par le pépiniériste. (agronomy-journal.org)
- In the same experiment, K84 reduced the incidence of galling by 94% in seedlings inoculated with a local agrocin-resistant A tumefaciens strain. (agronomy-journal.org)
- Agglutination of Erwinia stewartii strains with a corn agglutinin: correlation with extracellular polysaccharide production and pathogenicity. (microbiologyresearch.org)
- Plant genomes can be engineered by use of Agrobacterium for the delivery of sequences hosted in T-DNA binary vectors. (wikipedia.org)
- The research results indicated that cefotaxime sodium was suitable for inhibiting Agrobacterium in the screening and bud elongation stages. (researchsquare.com)
- A. tumefaciens has flagella that allow it to swim through the soil towards photoassimilates that accumulate in the rhizosphere around roots. (wikipedia.org)
- These data indicate that K84 could be used in Algeria despite the presence of agrocin- resistant strains. (agronomy-journal.org)
- This strain has a high degree of tolerance to chromium and other metals, may be radiation resistant and is a hydrocarbon degrader. (up.ac.za)
- Within the octopine/cucumopine group, two main strain types were identified: 'large TA' strains with a TA region resembling the TL region of the biotype I octopine strain Ach5 and 'small TA' strains with a similar T region organization as the 'large TA' strains but with a large internal TA deletion. (nih.gov)
- Agrobacterium tumefaciens serves as the outgroup in this tree. (cdc.gov)
- La protection par K84 des plants inoculés avec une souche résistante à l'agrocine 84 suggère l'existence en plus de la production d'agrocine, d'autres mécanismes favorisant la performance de cet antagoniste. (agronomy-journal.org)
- The wide variety of plants affected by Agrobacterium makes it of great concern to the agriculture industry. (wikipedia.org)
- Two engineered S. cerevisiae strains, LGA-1 and LGA-C, with high titers of d -glucaric acid were obtained. (biomedcentral.com)
- In the presence of opines, A. tumefaciens produces a diffusible conjugation signal called 30C8HSL or the Agrobacterium autoinducer[citation needed]. (wikipedia.org)