AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsAnthropology, Education, Sociology and Social PhenomenaTechnology, Industry, AgricultureInformation ScienceHealth CareGeographicals
BiocatalysisEnzymes, Immobilizedomega-ChloroacetophenoneCells, ImmobilizedBiotechnologyBatch Cell Culture TechniquesEnzymesLipaseStereoisomerismEscherichia coliCatalysisOctanesBioreactorsKetonesMetabolic EngineeringIonic LiquidsBiodegradation, EnvironmentalRhodococcusRhizomucorGreen Chemistry TechnologySequence Analysis, DNADiatomaceous EarthRhizopusBiofuelsAspergillus ochraceusSubstrate SpecificityEnzyme StabilityProtein EngineeringSolventsAcetobacterOctanolsBioelectric Energy SourcesSpecies SpecificityFermentationPseudomonas putidaMolecular Sequence DataIndustrial MicrobiologyBiotransformationButanolsDirected Molecular EvolutionGenetic EngineeringRNA, Ribosomal, 16STemperatureCulture MediaStyreneFuraldehydeAmino Acid SequenceMice, Inbred StrainsDNA, RibosomalDNA, BacterialBacterial Typing TechniquesCloning, MolecularKineticsSoil MicrobiologyGenes, BacterialBacterial ProteinsOxygenasesEsterificationOxidation-ReductionBacteriaVirulenceFungiHydrogen-Ion ConcentrationRecombinant ProteinsXyloseBiomassEpoxy CompoundsHexuronic AcidsChitosanEsterasesMutationGlucuronic AcidAspergillus nigerMicrobial Sensitivity TestsAnti-Bacterial AgentsEscherichia coli ProteinsAlkynesCandidaPhenotypeSerotypingBase CompositionPlasmidsBiosensing TechniquesGenes, rRNAPolymerase Chain ReactionMixed Function OxygenasesThiophenesDrug Resistance, MicrobialHydroxylationHydrolysisSequence AlignmentGenome, BacterialAlcohol OxidoreductasesGenotypeGene Expression Regulation, BacterialModels, MolecularNucleic Acid HybridizationSequence Homology, Amino AcidEthanolRNA, Bacterial
Microbial strainsEnzymesProteinsEnzymeGenesYeast strainsCharacterizationRecombinantBacteriaBiotechnological applicationsEthanol productionOptimisationPseudomonasMetabolicFermentationProductionTolerantBacteriumEscherichiaColiAnalyticalBiodegradationGenomeSynthesisGrownChemicalsSoilPlasticsGeneCrudePromoterIndustrialEffectiveHighActivityDevelopCellProcess
Microbial strains2
  • Ginkgo's synthetic biology platform, enables you to develop functional proteins, enzyme biocatalysts, small molecule ingredients, and microbial strains for a variety of applications in the nutrition and wellness markets. (ginkgobioworks.com)
  • The discovery and optimisation of improved microbial strains and biocatalysts. (fiiro-ng.org)
Enzymes5
  • The many strains of R. oryzae produce a wide range of enzymes such as carbohydrate digesting enzymes and polymers along with a number of organic acids, ethanol and esters giving it useful properties within the food industries, bio-diesel production, and pharmaceutical industries. (wikipedia.org)
  • Carbonic anhydrase (CA, EC 4.2.1.1), an ancient enzyme and the fastest among many enzymes, is a useful biocatalyst for carbon capture use and storage (CCUS). (springeropen.com)
  • Extending the capabilities and uncovering the mechanisms of these new enzymes derived from natural iron-heme proteins provides a basis for discovering new biocatalysts for increasingly challenging reactions. (cam.ac.uk)
  • Eva Nordberg Karlsson and Rajni Hatti-Kaul, on the investigation of enzymes and recombinant bacteria strains, for the conversion of renewable feedstocks in value-added products. (lu.se)
  • Some of the enzymes which generate aromatic radicals that break down most prominent bacterial strains found were isolated and tax- the complex linkages present in lignin to compounds of lower onomically identified using 16S ribosomal RNA (rRNA) se- molecular weight. (lu.se)
Proteins1
  • These specific ethanol tolerance related traits/proteins/genes can be used by researchers for developing more ethanol tolerant biocatalysts for biomass to biofuel conversion. (usda.gov)
Enzyme6
  • This enzyme is an important biocatalyst in the food industry for the production of prebiotic ingredients. (cuvillier.de)
  • Interested in technologies and partners to accelerate research at BASF in the areas of agricultural and industrial biotechnology including industrial enzyme and biocatalyst engineering, strain engineering for bio-based chemical production, fermentation process optimization, trait research, breeding, crop protection and sustainable solutions. (autm.net)
  • Lipase is important enzyme used in the biotechnological process of hydrolysis of fats in almost all the relevant industries We have utilized the local oil-contaminated soil resources to search for efficacious bacterial strains that have excellent lipase activity. (microbiologyjournal.org)
  • Linking to Grand Challenge 1, the availability of rapidly optimised biocatalysts in sufficient scale is addressed by working closely with the enzyme supply chain. (futurebrh.com)
  • Interested in technologies and partners to accelerate Bioscience research at BASF in the areas of industrial biotechnology including industrial enzyme and biocatalyst engineering, strain engineering for bio-based chemical production, fermentation process optimization and microbiome research. (ebrc.org)
  • Seminar Title: Enzyme Discovery & Engineering to Create Suitable Biocatalysts Speaker: Prof. Uwe T. Bornscheuer, Institute of Biochemis. (oist.jp)
Genes4
  • Since rhodopsin apoproteins cannot absorb light energy, rhodopsins produced by prokaryotic strains lacking genes for retinal biosynthesis are hypothesized to be non-functional in cells. (go.jp)
  • The strains were isolated, optimized, and purified using standard microbiology protocols and were characterized at the molecular level using the biomarker 16s ribosomal RNA genes of the strains. (microbiologyjournal.org)
  • Further analysis of E. xiangfangensis Pb204's genome revealed that it possessed several unique metal resistance genes not found in the type strain. (wits.ac.za)
  • Here, we report a new strain with chromosomally integrated ethanologenic pathway under the control of a constitutive promoter, without recombinant plasmids and resistance genes. (biomedcentral.com)
Yeast strains1
  • This study aimed to isolate and identify aquatic yeast strains for their potential to form Zinc oxide nanoparticles (ZnONPs). (ac.ir)
Characterization3
  • The identified and isolated bacterial strains were confirmed as Bacillus subtilis strain RCPS3, and Bacillus fumarioli strain RCPS4 through molecular and computational characterization. (microbiologyjournal.org)
  • 2. Molecular Characterization of the identified strains through transformational studies for the enhancement of products. (fiiro-ng.org)
  • Hydrotreated Petro Star diesel has been generated for use in the characterization of biocatalyst strains. (doe.gov)
Recombinant3
  • In this scope, the presented thesis illustrates the integration of strain and process engineering using the example of producing the biosurfactant rhamnolipids with recombinant Pseudomonas putida KT2440. (rwth-aachen.de)
  • In order to further increase the production efficiency and simplify operations, we also constructed recombinant E. coli strains that co-expressed SPRHA2 and PBGL. (biomedcentral.com)
  • My research is on the development of biocatalysts, combining computational methods and recombinant technology. (lu.se)
Bacteria3
  • 2003). Cockroaches have been searched as transmitters of pathogenic bacteria and carrries of multiple antibiotic resistance strains (Fotedar et al . (pjmonline.org)
  • This study investigated the potential of aquatic bacteria for their ability as a biocatalyst to synthesized Fe2O3 nanoparticles using iron precursor, FeCl3. (ac.ir)
  • Abstract Starting from mature vegetable compost, four bac- the utilization of these bacteria as cell factories for upgrading terial strains were selected using a lignin-rich medium. (lu.se)
Biotechnological applications3
  • Other harmless strains of cyanobacteria have great potential for biotechnological applications and for this reason are much sought-after. (tugraz.at)
  • In one area, the use of cyanobacteria as biocatalysts for light-driven biotechnological applications is being examined and tested in a range of industrially relevant model reactions. (tugraz.at)
  • Bacterial Gamma-Glutamyl Transpeptidase, an Emerging Biocatalyst: Insights Into Structure-Function Relationship and Its Biotechnological Applications. (proteinlounge.com)
Ethanol production1
  • Our strain has attractive features for industrial settings and represents a relevant improvement in the existing ethanol production biotechnologies. (biomedcentral.com)
Optimisation1
  • a) Accelerated discovery and delivery of biocatalysts and processes, with faster optimisation to enable their use earlier in manufacturing programmes and allow realisation of the full benefits for 'right first time' biomanufacturing solutions. (ukri.org)
Pseudomonas4
  • They isolated a Pseudomonas azelaica strain HPB1 which was able to degrade OPP through the production of 2,3-dihydroxybiphenyl. (nature.com)
  • Based on the results of phylogenetic similarity of 16SrDNA gene sequences, strain DB17 was identified to be Pseudomonas putida. (omicsonline.org)
  • 2010 ). Escherichia coli is not an ideal reporter strain due to low resistance to the harsh conditions in polluted water and soil compared with other organisms such as Pseudomonas , Acinetobacter, or Bacilli species that resist these conditions. (springeropen.com)
  • Bacterial metabolism selected lignin model compounds (vanillin, vanillate, 4- hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a Introduction culture collection. (lu.se)
Metabolic5
  • However, the microbial metabolic pathway of the fungicide and the genetic systems driving its degradation by strain P3 remain unknown. (nature.com)
  • Therefore, elucidation of the microbial metabolic pathway of OPP is a prerequisite for the downstream exploitation of strain P3 in any environmental application. (nature.com)
  • Metabolic modeling, which helps making sense of the metabolism in a biological network, is an important tool for engineering biocatalysts, with applications in biofuels, drug design, microbial community analysis, and personalized medicine. (nature.com)
  • In metabolic engineering, strains are modified and controlled at the genome level through the transcriptome, and the effects are observed at the fluxome level, which accounts for the range of metabolic reactions in an organism. (nature.com)
  • Classically, model-based strain design has relied on tools that use the DNA sequence of an organism and homology with well-studied organisms to infer a network of metabolic reactions that happen inside a cell of that organism, which is called a genome-scale model (GEM). (nature.com)
Fermentation1
  • The strain showed extreme stability in 1-month subculturing, with CWP fermentation performances similar to the ethanologenic plasmid-bearing strain. (biomedcentral.com)
Production7
  • Based on a genetically defined model strain with plasmid based production of the gene for the green fluorescent protein ( GFP ), at first, the optimization of the basic culture parameters pH-value, temperature and dissolved oxygen concentration was performed. (cuvillier.de)
  • Finally, the results from the model strain were successfully transferred to the production of a homologous protein, the levansucrase SacB. (cuvillier.de)
  • Microbial sources are regularly used as reliable biocatalysts sources which are often used in the process and production industry. (microbiologyjournal.org)
  • We were successful in identifying two such bacterial sources, namely, Bacillus subtilis strain RCPS3 and Bacillus fumarioli strain RCPS4, responsible for lipase production from oil effluent contaminated soil of Telangana. (microbiologyjournal.org)
  • This is the first report of these two strains from this part of India that are involved in lipase production. (microbiologyjournal.org)
  • It could be speculated that the results of the study can hopefully introduce the inherent capabilities of aquatic microbes as safe, simple, and effective biocatalysts in the production of Fe2O3 nanoparticles. (ac.ir)
  • We previously engineered an Escherichia coli strain for optimized bioethanol production from lactose-rich wastewater like concentrated whey permeate (CWP), a dairy effluent obtained from whey valorization processes. (biomedcentral.com)
Tolerant1
  • Genome re-sequencing and subsequent reverse genome engineering enabled the construction of tolerant strains capable of producing rhamnolipids in the presence of 1-octanol. (rwth-aachen.de)
Bacterium3
  • An effective profenofos and λ-cyhalothrin degrading bacterium (strain DB17) was isolated from soil samples collected from Qassim region, Saudi Arabia. (omicsonline.org)
  • In summary, a new CA with high thermal and alkaline stability was identified from a general bacterium, demonstrating an effective strategy for discovering new and useful biocatalysts. (springeropen.com)
  • The results showed that cell-free extract (CFE) of the bacterium strain can produce the rod-shaped Fe2O3 nanoparticles with mean edge lengths of 80.2 nm and mean diameters of 25.5 nm, after being exposed to FeCl3 solution (10 mM), at an optimum pH of 6 and an optimum temperature of 28 °C, after 96 hours of incubation at 150 rpm. (ac.ir)
Escherichia2
  • Here, an Escherichia coli reporter strain expressing a chromoprotein capable of interacting with soil or water samples and responding to DNA damaging compounds is validated. (springeropen.com)
  • Chang JS, Kuo TS, Chao YP et al (2000) Azo dye decolorization with a mutant Escherichia coli strain. (vegetosindia.org)
Coli2
  • Reporter strains developed in E. coli have been used for determining the dynamics in ecotoxicology due to the in-depth knowledge of this organism (Robbens et al. (springeropen.com)
  • Also, several molecular tools are available for E. coli that generate this organism a highly flexible chassis for developing reporter strains. (springeropen.com)
Analytical2
  • Our findings suggest that chromogenic reporter strains can be applied for simple screening, thus reducing the number of samples requiring analytical techniques. (springeropen.com)
  • Title: Analytical Validation of Variational Models for Epitaxially Strained Thin Films: A Review Abstract: Films self-assembled by epitaxial growth o. (oist.jp)
Biodegradation1
  • As a result, these bacterial strains may be utilized for biodegradation of endosulfan polluted soil and environments. (pjmonline.org)
Genome2
  • Currently, DOE and JGI is sequencing the genome of this strain. (usda.gov)
  • With the above mentioned unique characteristics, plus the genome sequence data, this strain can be used by researchers for developing new biocatalysts that convert biomass derived sugars into ethanol and other value-added products. (usda.gov)
Synthesis4
  • The objective of this work was contributed in developing techniques to obtain immobilized biocatalysts with low cost and high catalytic activity to be applied in the biodiesel synthesis by ethanol route. (usp.br)
  • The following reaction parameters: growth media, biocatalyst ratio, temperature, pH and gold ion concentration were altered to determine their influence on AuNP synthesis by Enterobacter sp. (wits.ac.za)
  • The present study pioneered the capabilities of the native aquatic strain Rhodotorula pacifica for the extracellular synthesis of ZnONPs with CFE strategy. (ac.ir)
  • Extracellular synthesis of Fe2O3 nanoparticles by this strain was investigated under the optimal conditions. (ac.ir)
Grown1
  • To produce uniformed spherical AuNPs, within a size range of 2 to 15 nm, the following parameters were identified: cell biocatalyst grown LB at a pH of 3, incubated at 37 °C with a chloroauric acid concentration of 1 mM for 24 hours. (wits.ac.za)
Chemicals1
  • Thus, robust and improved biocatalysts are needed to convert lignocellulosic biomass hydrolysates into fuels and valuable chemicals. (usda.gov)
Soil2
  • Using bacterial cells as reporter strains represents an advantage for detecting pollutants present in soil or water samples. (springeropen.com)
  • This strain can be used simply by applying soil or water samples directly and rendering activation upon DNA damage. (springeropen.com)
Plastics1
  • One of my recent projects aims to develop biocatalysts for recycling synthetic polymers, including plastics and textiles. (lu.se)
Gene1
  • Synthetic biology is highly important to industrial biomanufacturing, considering its tools and techniques (i.e. gene editing and strain engineering) that allow white biotechnology to push into new applications. (specchemonline.com)
Crude1
  • Numerous other factors need to be evaluated to determine the economic viability of certain white biotechnology projects, from internal factors such as process yield, ease of scale, and biocatalyst choice to external factors such as government regulations, crude oil prices, and the green premium. (specchemonline.com)
Promoter1
  • The reporter strain generates a qualitative signal and is based on the expression of the coral chromoprotein AmilCP under the control of the recA promoter. (springeropen.com)
Industrial2
  • Biocatalysts remain the best solution till now in mediating an industrial process through accelerating or decelerating a particular reaction. (microbiologyjournal.org)
  • The goal of this Grand Challenge is to engineer biocatalysts up to an order of magnitude faster than the current industrial timeframe. (futurebrh.com)
Effective1
  • The developed reporter strain reported here is effective for the detection of DNA damaging agents present in soils samples. (springeropen.com)
High3
  • Growth-Based, High-Throughput Selection for NADH Preference in an Oxygen-Dependent Biocatalyst. (acs.org)
  • Using gas chromatography coupled to mass spectrometry (GC-MS), also revealed that all the strains included in this study produced high levels of isoamyl acetate. (sciforum.net)
  • A yeast strain, NS02, with high tolerance against zinc ion (5.25 mM) was isolated using the enrichment technique and was selected as efficient candidate for the biosynthesis of ZnONPs under cell-free extract (CFE) strategy. (ac.ir)
Activity2
  • Several biocatalysts have been tested for their activity in partially treated diesel fuel. (doe.gov)
  • This thesis describes a series of investigations into the hydantoin-hydrolysing activity of bacterial strains RU-KM1 and RU-OR, which were previously isolated for their ability to hydrolyse hydantoins to amino acids. (ru.ac.za)
Develop1
  • Specific objectives are to 1) develop a biocatalyst that will remove a large portion of the sulfur in diesel fuel at commercially acceptable rates and 2) develop design specifications and a cost estimate for a 5,000 barrel-per-day biodesulfurization demonstration unit. (doe.gov)
Cell2
  • Furthermore, spectroscopic analyses of the cell lysate and HPLC-MS/MS analyses revealed that this strain contained an endogenous retinal. (go.jp)
  • A refinement of cultivation parameters was required to enhance functionality of the extraction, in turn influencing the performance of the whole-cell biocatalyst. (rwth-aachen.de)
Process1
  • Integrated biochemical engineering : strain and process engineering for the pro. (rwth-aachen.de)