Strains antibodies neutralizing. Medical search. Frequent questions

Recently developed cell-free tools can measure antibody titers but cannot necessarily evaluate neutralization, and none of the currently available tools have estimated neutralization activity against the emerging set of SARS-CoV-2 VOCs 8 . (nature.com)
Individuals who received two vaccine doses had significantly reduced NtAb titers against all SARS-CoV-2 strains, compared to those infected and then vaccinated with one or more doses, those vaccinated with two or more doses and then infected, or those who received three doses of vaccine. (covid19immunitytaskforce.ca)
Participants vaccinated with two or three doses of vaccine who were then infected had the highest NtAb titers against all SARS-CoV-2 strains tested. (covid19immunitytaskforce.ca)
The animals were intramuscularly administered different vaccine doses, and the control and enzyme-linked immunosorbent assays (ELISA) and pseudovirus neutralization assays were used to determine the serum antibody titers. (news-medical.net)
During the live virus neutralization experiments, plaque reduction neutralization assays were used to measure the macaque serum samples, while cytopathic effect neutralization assays were used to determine the neutralizing antibody titers in hamster serum samples. (news-medical.net)
The data suggests increased immunogenicity associated with ARCT-154 at Day 29 with 1.43-fold higher geometric mean titers of neutralizing antibodies against the vaccine strain versus Comirnaty. (businesswire.com)

A much more promising strategy is to use recombinant monoclonal antibodies (mAbs) against influenza and several are currently in clinical development ( 9 - 13 ). (frontiersin.org)
This discovery led her to develop methods to generate anti-HIV human monoclonal antibodies (mAbs) from the blood cells of HIV-infected individuals. (wikipedia.org)
Relatively few monoclonal antibodies were available and access to more could provide additional material for use in diagnosis. (who.int)
The Committee recommended the establishment of a time-limited programme for production of monoclonal antibodies. (who.int)

In this study, we presented a neutralizing monoclonal antibody (mAb) based competitive enzyme-linked immunosorbent assay (cELISA) with the emphasis on the replacement of VNT for C-strain post-vaccination monitoring. (biomedcentral.com)

A CITF-funded study, published in preprint and not yet peer-reviewed, found that vaccination was associated with higher neutralizing activity (the ability of antibodies to block entry of the virus into the cell) against pre-Omicron variants than against Omicron sub-lineages. (covid19immunitytaskforce.ca)
Vaccination followed by infection was associated with higher neutralizing activity against Omicron sub-lineages than vaccination alone. (covid19immunitytaskforce.ca)
This study highlights the importance of up-to-date vaccination for food and retail workers to stimulate an effective neutralizing response against Omicron variants. (covid19immunitytaskforce.ca)
Trials of the bivalent COVID jab showed an eight-fold rise in neutralizing antibodies against Omicron. (weforum.org)
NEW YORK & MAINZ, Germany--(BUSINESS WIRE)-- Pfizer Inc. (NYSE: PFE) and BioNTech SE (Nasdaq: BNTX) today announced results from an initial laboratory study demonstrating that serum antibodies induced by the Pfizer-BioNTech COVID-19 Vaccine (BNT162b2) neutralize the SARS-CoV-2 Omicron variant after three doses. (pfizer.com)
A recent study published in Nature Microbiology identified a monoclonal antibody (mAb) neutralizing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variants. (news-medical.net)
The latest VOC, Omicron, carries 37 mutations in its spike protein, most of which lie within the receptor-binding domain (RBD), the target for neutralizing antibodies (nAbs). (news-medical.net)
The World Health Organization (WHO) has classified the SARS-CoV-2 Alpha, Beta, Gamma, Delta, and Omicron variants as variants of concern because they carry mutations in their spike protein that increase their transmissibility and ability to evade vaccine-induced immunity, as compared to the ancestral Wuhan strain. (news-medical.net)
The third dose significantly increased the neutralizing antibody levels against the omicron strain, the company said in a statement released Wednesday. (mcknights.com)
Although two doses of the vaccine may still offer protection against severe disease caused by the omicron strain, it's clear from these preliminary data that protection is improved with a third dose of our vaccine," said Albert Bourla, CEO. (mcknights.com)
Investigators found a significant drop in the response of vaccine-produced antibodies to omicron when compared with the response produced to delta and the original coronavirus variant. (mcknights.com)
Superiority of ARCT-154 in neutralizing antibody response against SARS-CoV-2 Omicron BA.4/5 variant was also demonstrated as a key secondary endpoint. (businesswire.com)
The geometric mean fold rise (GMFR) in neutralizing antibodies remained greater than 10-fold above baseline for one year across a panel of variants (ref: Figure), including Omicron BA.1. (businesswire.com)
The first lab study of the super-variant in South Africa last night found that people given two doses of Pfizer's vaccine produce forty times less antibodies when exposed to Omicron. (dailymail.co.uk)
Moderna is advancing the trial into its next phase as research published Wednesday in the New England Journal of Medicine found that a booster dose of the vaccine remained durable against the Omicron variant but did show signs of waning antibody protection. (cnn.com)
We are reassured by the antibody persistence against Omicron at six months after the currently authorized 50 μg booster of mRNA-1273. (cnn.com)
The new study says the Moderna Covid-19 booster shot remains durable against the Omicron variant, but the antibody protection wanes and is six times lower six months after getting boosted. (cnn.com)
The study also found that the neutralizing antibody levels declined against the Omicron variant much more rapidly than against the dominant strain of the virus that was circulating two years ago. (cnn.com)
Every time scientists have thought they'd subdued one strain of the virus - be it alpha, beta, delta, or omicron - another variant or subvariant emerged a short while later. (mit.edu)

Knowledge of individual immunity to SARS-CoV-2 is useful not only to determine personal actions but also to guide early therapy of patients and evaluate the efficacy of antibody treatment and vaccines over time against emerging viral variants of concern (VOCs) 3 . (nature.com)
The UK's chief scientific adviser Sir Patrick Vallance said it could be the case that the new strains reduce the efficacy of vaccines, but more studies were needed. (yorkshireeveningpost.co.uk)
The company reported 96% efficacy against the original coronavirus, 86.3% efficacy against the U.K. strain, and 55.4% efficacy against the South African variant. (fool.com)
The utility and technical advantages of synthetic nucleic acid platforms (SNAP), including their efficacy, safety, speed of development, and ease of vaccine manufacture, were revealed during the COVID-19 pandemic, thus instilling hope that these technologies can be leveraged for the development of an effective HIV vaccine and broadly neutralizing antibody (bNAb) delivery. (nih.gov)
Among those who'd received a single dose of vaccine, antibodies demonstrated moderate efficacy against the original COVID-19 virus, limited efficacy against the U.K. variant, and virtually none against the South African one. (nymag.com)
The South African version can evade the action of neutralizing antibodies from previous COVID-19 and favor reinfection, which means the mutation may also reduce vaccine efficacy. (bgr.com)
Here, we describe the safety, robust immunogenicity, and potent efficacy elicited in rhesus macaques by a modified vaccinia virus Ankara (MVA) vector expressing a full-length SARS-CoV-2 spike (S) protein (MVA-S). MVA-S vaccination was well tolerated and induced S and receptor-binding domain (RBD)-binding IgG antibodies and neutralizing antibodies against SARS-CoV-2 and several variants of concern. (frontiersin.org)
As the B.1.617.1 variant continues to evolve, it will be important to monitor how additional mutations within the spike impact antibody resistance, viral transmission and vaccine efficacy. (biorxiv.org)

With the availability of coronavirus disease 2019 (COVID-19) vaccines, the rise of more transmissible and pathogenic virus mutants 1 and known time-dependent declines in immunity following infection 2 , there is a need to determine the degree of serological antibody protection from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). (nature.com)
IMPORTANCE Rotavirus live- attenuated vaccines generate broadly heterotypic protection , and B- cells isolated from adults encode antibodies that are broadly protective in mice . (bvsalud.org)
Building on her correlation of levels of V2 antibodies with reduced infection rates in the RV144 vaccine trial, Zolla-Pazner and her colleagues have spent the last 10 years developing "designer vaccines," i.e. (wikipedia.org)
Current human immunodeficiency virus-1 (HIV-1) vaccines elicit strain-specific neutralizing antibodies. (duke.edu)
The results demonstrated that the recombinant virus was as stable and effective as the parental strain for the preparation of inactivated vaccine, suggesting the potential application of this strategy to make genetically engineered FMDV vaccines. (ias.ac.in)
The premise of standard Covid-19 vaccines, such as those produced by Moderna and Pfizer, is to activate the part of the immune system that releases neutralizing antibodies. (mit.edu)
For years, scientists have been on the hunt to develop vaccines that would protect against all or most strains of particularly virulent viruses such as influenza. (genengnews.com)
New combination vaccines should induce similar or superior levels of neutralizing antibody in serum for individual protection against paralytic disease and mucosal immunity that effectively decreases viral replication in the intestine and pharynx for population protection against transmission of poliovirus. (who.int)

To achieve this goal, we designed an assay that focuses on antibodies competing with RBD:ACE-2 interactions as a proxy for antibody neutralization 8 (Fig. 1a,b ). (nature.com)
It is a reliable tool for sero-monitoring of C-strain vaccination campaign because it is a rapid, simple, safe and cost effective assay that can be used to monitor vaccination-induced immune response at the population level. (biomedcentral.com)
the individual had the highest antibody levels, with a broad range against several variants in a previous neutralization assay. (news-medical.net)
Further, characterization of the recombinant virus by immunofluorescence, microneutralization test (MNT), antigen ELISA, RT-PCR, plaque assay and electron microscopy revealed similarity to the parental strain. (ias.ac.in)
In this study, using a live virus assay, we describe the neutralizing antibody response to the B.1.617.1 variant in serum from infected and vaccinated individuals. (biorxiv.org)
Antibodies against lyssaviruses were detected by enzyme-linked immunosorbent assay in 144 (14.7%) of 981 serum samples. (cdc.gov)
The 'gold standard' method to assess humoral antibody responses fol owing vaccination is the neutralization assay. (who.int)
Laboratory testing with a rapid conducted at that time showed that 6.1% of the residents assay suggested that a dengue virus (DENV) was the caus- in nearby regions of Uganda had specifi c antibodies to ative agent. (folkhalsomyndigheten.se)

Serological antibody tests (ideally home-based diagnostics) are critical to evaluate the response to vaccination and viral infection 2 . (nature.com)
Antibody neutralizing capabilities are traditionally measured in cell-based live viral infection assays that require BSL3 facilities 6 . (nature.com)
There is, however, scarce information about to what extent human infection with 1 HFMD-causing enterovirus serotype can elicit (cross-)neutralizing antibodies against homotypic and heterotypic enterovirus serotypes. (cdc.gov)
Our findings demonstrate AAV delivery of cross-subtype neutralizing nanobodies may be an effective strategy to prevent influenza infection and provide long-term protection independent of a host induced immune response. (frontiersin.org)
Zolla-Pazner's work has focused on how the immune system responds to the human immunodeficiency virus (HIV) and, in particular, how antibodies against the viral envelope develop in the course of infection. (wikipedia.org)
Zolla-Pazner's work helped establish the central role of antibodies in protection from HIV infection. (wikipedia.org)
Her research indicated that high levels of antibodies to the second variable region of the virus envelope protein, V2, correlated with a reduced rate of infection. (wikipedia.org)
She initiated systemic testing to enumerate CD4 and CD8 cells and antibodies in order to diagnose HIV infection and follow the effects of treatment with antiretroviral drugs. (wikipedia.org)
Studies of these recombinant vaccine constructs in both rabbits and nonhuman primates have demonstrated that they induce antibodies that react with the envelopes of diverse viruses within the various subgroups of HIV and have biologic activities that have been associated with protection from infection. (wikipedia.org)
Accordingly, here we analyse antibodies and T cells of a recently vaccinated, UK cohort, alongside those recovering from natural infection in early 2020. (nature.com)
These spikes have sites that are vulnerable to powerful antibodies , which block laboratory infection of human cells by more than 90 percent of tested HIV strains from around the globe. (nih.gov)
H3N2 influenza infection elicits more cross-reactive and less clonally expanded anti-hemagglutinin antibodies than influenza vaccination. (duke.edu)
During the recent H1N1 influenza pandemic, excess morbidity and mortality was seen in young but not older adults suggesting that prior infection with influenza strains may have protected older subjects. (duke.edu)
After infection with diverse strains of H1N1 influenza, treated mice showed minimal weight loss and lung inflammation. (caltech.edu)
Researchers have identified 'broadly neutralizing' antibodies that protect against infection by multiple, distantly related alphaviruses-including Chikungunya virus (above)-that cause fever and debilitating joint pain. (genengnews.com)
A new study by researchers at the Washington University School of Medicine in St. Louis (WUSTL) has identified broadly neutralizing antibodies that protect against infection in mice from multiple, distantly related alphaviruses-including Chikungunya virus (CHIKV)-that cause fever and debilitating joint pain. (genengnews.com)
Using in vitro cell culture studies, Dr. Diamond and his team observed that antibodies which recognized the alphavirus epitope also protected against infection by multiple alphaviruses. (genengnews.com)
Although low levels of neutralizing antibodies have been detected in kangaroos, wallabies, possums, horses, cats and dogs after experimental infection, the detected viraemia is considered too low for an insect vector to acquire the virus. (who.int)

In this clinical trial, the AAV vector designed by MGH investigators carries the genetic sequence for what is called a broadly neutralizing HIV-1 antibody that blocks HIV's ability to bind to CD4, an immune cell's receptor that HIV targets before infecting the cell. (sciencedaily.com)
Co-evolution of a broadly neutralizing HIV-1 antibody and founder virus. (duke.edu)

The following three companies are tackling new variants of concern -- strains that are more transmissible or more dangerous than the original coronavirus. (fool.com)
A modified vaccine antigen containing substitution mutations shared by the variants of concern could elicit broadly neutralizing antibodies against the circulating variants. (news-medical.net)
Vero-E6 cells were used to propagate SARS-CoV-2 viruses of the ancestral strain and the variants of concern. (news-medical.net)

We aimed to develop a sensor technology that can quantitatively measure nAb responses against different isolates of SARS-CoV-2, be adapted for an all-in-solution multiwell format and provide rapid results in 1 hour, which is faster than established ELISA assays measuring SARS-CoV-2 antibody titer (~6 hours) or cell-based neutralization assays (~one to several days). (nature.com)
Knowledge of neutralizing antibody responses among its pathogens is essential to inform vaccine development and epidemiologic research. (cdc.gov)
There was no significant difference in participants' neutralizing responses in terms of sex, age, chronic disease, and whether they smoked or vaped. (covid19immunitytaskforce.ca)
The vaccine was safe and well tolerated, induced potent serum IgG & IgA responses waning post last vaccination, and low and intermittent mucosal IgG, but did not induce neutralizing activity neither in serum nor vaginal samples. (europa.eu)
The animal models used to test immunogenicity and antibody responses to viral challenge included BALB/c mice, rhesus macaques, and Golden Syrian hamsters. (news-medical.net)
The study compared immune responses between ARCT-154 and Comirnaty booster doses (original strain) in Japanese adults (N = 828 and randomized 1:1 to ARCT-154 and Comirnaty) that were previously immunized with two doses of mRNA COVID-19 vaccine then a third booster dose of Comirnaty at least 3 months prior to enrollment. (businesswire.com)
Allo-Specific Humoral Responses: New Methods for Screening Donor-Specific Antibody and Characterization of HLA-Specific Memory B Cells. (duke.edu)
The recent emergence of SARS-CoV-2 variants that are less sensitive to neutralization by convalescent sera or vaccine-induced neutralizing antibody responses has raised concerns. (biorxiv.org)

V2- and V3-scaffold proteins that focus the immune response on the production of antibodies specific for these two regions of gp120. (wikipedia.org)

The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein binds to the angiotensin-converting enzyme 2 (ACE-2) receptor on target cells and is the immunodominant target of neutralizing antibodies (nAbs) identified from convalescent and postvaccination plasma 3 . (nature.com)
Passive transfer of serum antibodies from convalescent patients has been used in the past ( 7 , 8 ), however, this approach is of limited use in a global pandemic emergency. (frontiersin.org)

Pfizer has now tested 16 different mutations in the strains and found that none of them have had any impact on how the vaccine works. (yorkshireeveningpost.co.uk)
Since the emergence of the new coronavirus, called SARS-CoV-2, several researchers have proposed that there is more than one strain, and that mutations have led to changes in how infectious and deadly it is. (medicalnewstoday.com)
On top of that, three new coronavirus strains are spreading rapidly, including the UK, South African, and Brazilian mutations. (bgr.com)
These variants contain mutations arising within the spike protein that are known to increase resistance to antibody neutralization 3 . (biorxiv.org)

These rare mAbs bind to functionally conserved epitopes such as those in the hemagglutinin (HA) stem, thereby providing strain independent protection. (frontiersin.org)
Our data confirm that VOC, particularly those such as B.1.351 with substitutions at residues 484 and 417, escape neutralization by antibodies directed to the ACE2-binding Class 1 and the adjacent Class 2 epitopes but are susceptible to neutralization by the generally less potent antibodies directed to Class 3 and 4 epitopes on the flanks of the RBD. (nature.com)
Neutralizing antibodies that target epitopes conserved among many strains of influenza virus have been recently isolated from humans. (caltech.edu)
Typically, individuals who are infected with alphaviruses produce antibodies against many viral epitopes, some of which are not protective-setting up a weak immune response scenario that allows the virus time to replicate and exert its pathogenic effects. (genengnews.com)

Assays that measure antibody titer and neutralizing capability exist but are not compatible with home use. (nature.com)
Traditional affinity-based immunoassays, such as enzyme-linked immunosorbent assays (ELISAs) 5 , can quantitatively measure antibody titer, but due to inherent complexity and instrumentation, they require a centralized laboratory for diagnostics. (nature.com)
Any detectable titer of neutralizing antibody against poliovirus is considered protective against clinical paralytic diseases. (who.int)

The structure of an attenuated human rotavirus isolate ( CDC -9) bound with the Fab fragment of a broadly heterotypic protective antibody shows that protection is probably due to inhibition of the conformational transition in the viral spike protein (VP4) critical for viral penetration, rather than to inhibition of receptor binding. (bvsalud.org)
they also validated her hypothesis that antibodies to variable regions could be protective-a highly controversial point until then. (wikipedia.org)
Here we demonstrate that adeno-associated viruses (AAV) encoding two such broadly neutralizing antibodies are protective against diverse influenza strains. (caltech.edu)
Conversely, people who are immunized with proteins expressing the key epitope identified by the WUSTL researchers should be able to rapidly produce proper protective antibodies, thereby short-circuiting the disease process. (genengnews.com)

A novel cELISA was established and optimized based on the strategy that 6B211 can compete with C-strain induced neutralizing antibodies in pig serum to bind capture antigen C-strain E2. (biomedcentral.com)
Her lab also described how antibodies to V2 and V3 could bind and neutralize viruses from all over the globe, despite extreme variations in amino acid sequences. (wikipedia.org)
Even so, HIV vaccine designers might have succeeded by now, had they been able to study the structure of the entire Env protein at atomic-scale-in particular, to fully characterize the sites where the most effective virus-neutralizing antibodies bind. (scripps.edu)
The findings from this study were published online recently in Cell through an article entitled "Broadly Neutralizing Alphavirus Antibodies Bind an Epitope on E2 and Inhibit Entry and Egress. (genengnews.com)

The problem with that approach is that the target keeps changing" - the spike protein itself can vary among different viral strains - "and that can make the vaccine ineffective," says David Gifford, an MIT professor in electrical engineering and computer science and biological engineering, as well as a coauthor of the Frontiers paper. (mit.edu)

Serum from mice that received a single intramuscular AAV injection efficiently neutralized all H1, H2 and H5 influenza strains tested. (caltech.edu)

The SARS-CoV-2/Finland/1/2020 virus strain was isolated, the genome showing a single nucleotide substitution to the reference strain from Wuhan. (eurosurveillance.org)

First, the research team screened serum samples from a cohort of more than 100 donors for the presence of antibodies against spike. (news-medical.net)
Thirty of 187 serum samples contained neutralizing antibodies against different lyssaviruses. (cdc.gov)

The overall objective of EuroNeut-41 was to design a vaccine that raises antibodies able to prevent HIV cell fusion by blocking the virus fusion protein gp41 in its pre-fusogenic conformation. (europa.eu)
HIV-1 envelope gp41 broadly neutralizing antibodies: hurdles for vaccine development. (duke.edu)

Induction of binding Abs was measured by ELISA and neutralizing activity by PBMC and TZM-bl assays in all serum and vaginal samples. (europa.eu)
Next, in live virus cytopathic effect assays, the authors noted a potent neutralizing activity of P2G3 against the D614G strain and VOCs. (news-medical.net)
Further, they tested P2G3 activity in antibody-dependent cellular phagocytosis (ADCP) and cytotoxicity (ADCC) assays. (news-medical.net)
Geometric mean fold rise (GMFR) of neutralizing antibodies against SARS-CoV-2 variants (versus pre-booster levels) after ARCT-154 booster vaccination measured by validated pseudoviral microneutralization assays (N=12). (businesswire.com)

Sera from H. pylori-infected persons neutralized the cytotoxins produced by multiple H. pylori strains, but failed to neutralize trimethylamine-induced cell vacuolation. (jci.org)
There is a lot of emphasis on identifying and understanding broadly neutralizing antibodies for other viruses-HIV, hepatitis C virus, dengue virus, influenza virus-but most of those antibodies neutralize different strains of the same virus," explained senior author Michael Diamond, M.D., Ph.D., a professor of medicine and director of the Division of Infectious Diseases and Vaccine Development in the Center for Human Immunology and Immunotherapy Programs at WUSTL. (genengnews.com)
What we've identified here are antibodies that actually neutralize several different alphaviruses. (genengnews.com)

The biosensor is a switchable, caged luciferase-receptor-binding domain (RBD) construct that detects serum-antibody interference with the binding of virus RBD to angiotensin-converting enzyme 2 (ACE-2) as a proxy for neutralization. (nature.com)

This novel cELISA is a rapid, simple, safe and cost effective approach for detection of C-strain CSF vaccine-induced immune response. (biomedcentral.com)
A third lockdown was imposed in England on Wednesday (6 January) in response to the rapid spread of the mutated strain found in the South East , which has now spread more widely across the UK. (yorkshireeveningpost.co.uk)
Interestingly, there was no significant difference in the neutralizing response in terms of sex, age, chronic diseases, and whether food and retail workers smoked or vaped. (covid19immunitytaskforce.ca)
Zolla-Pazner's research indicated that the search for an effective vaccine should focus not only on inducing specific types of antibodies but on eliciting a durable antibody response, as post-hoc analysis showed the levels of antibodies waned within 6 months of the last dose of the vaccine. (wikipedia.org)
Three of the eight participants showed signs of an anti-drug antibody response directed against a portion of VRC07, and this response appeared to decrease the production of VRC07 in two of the participants. (sciencedaily.com)
Serum neutralizing antibody response to the vacuolating cytotoxin of Helicobacter pylori. (jci.org)
The primary endpoint was achieved in the ARCT-154 Phase 3 booster vaccine study, demonstrating non-inferiority of immune response against SARS-CoV-2 ancestral strain compared to Comirnaty. (businesswire.com)
A Prevalent Focused Human Antibody Response to the Influenza Virus Hemagglutinin Head Interface. (duke.edu)
Neutralising antibody response appeared within 9 days along with specific IgM and IgG response, targeting particularly nucleocapsid and spike proteins. (eurosurveillance.org)
But the T cells in these samples produced a robust response against all three strains. (nymag.com)
Antibody response after severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccination is impaired in kidney transplant recipients. (lww.com)
If you can make an antibody response against this region, you may be able to protect against many viruses in the family," noted Dr. Diamond. (genengnews.com)
The characteristics of this response would include production of neutralizing antibodies, generation of a T-cell response, and avoidance of immune-enhanced disease4. (who.int)

Laboratory studies found that the samples had neutralising levels of antibodies which appeared to work against N501Y in the new strains. (yorkshireeveningpost.co.uk)
This work represents the first successful AAV-based production of a monoclonal antibody of any kind in people," says co-author Alejandro B. Balazs, PhD, who created the vector used in the trial and is a principal investigator at the Ragon Institute of MGH, MIT and Harvard, where his laboratory is continuing to develop this technology. (sciencedaily.com)

There is clearly an urgent need for additional approaches and antibodies present new opportunities for both therapeutic and prophylactic intervention. (frontiersin.org)
SNAP might also provide a means to persistently deliver prophylactic or therapeutic levels of antibodies or antibody derivatives, thereby supplying affordable, timely and sustainable global access to these HIV interventions. (nih.gov)

One monoclonal antibody (6B211) which has potent neutralizing activity against C-strain was generated. (biomedcentral.com)

We have mapped the epitope of one such antibody by determining the high-resolution cryo-EM structure of its antigen -binding fragment (Fab) bound to the virion of a candidate vaccine strain , CDC -9. (bvsalud.org)
In addition, a marker was inserted into the hemolysin gene locus (hlyA) to enable differentiation of the vaccine strain from wild type V. cholerae O1. (rxlist.com)
The vaccine strain is grown in fermentors under controlled conditions in medium containing casamino acids, yeast extract, mineral salts, and an anti-foaming agent. (rxlist.com)

Her lab isolated and described human mAbs capable of neutralizing the infectivity of the virus, and, later, isolated a mAb with strong neutralizing potency targeting a complex conformational region on the surface of the virus' envelope protein composed of V2 and V3. (wikipedia.org)
In comparison, the neutralizing potency of P2G3 against BA.1 and BA.2 variants far exceeded that of currently authorized mAbs. (news-medical.net)

Among study participants, neutralizing antibody (NtAb) levels in the blood were similar against the ancestral SARS-CoV-2 strain and the Delta variant. (covid19immunitytaskforce.ca)
In subjects aged 11 to 18 years, lower levels for antibodies to pertactin (PRN) were observed when BOOSTRIX was administered concomitantly with meningococcal conjugate vaccine (serogroups A, C, Y, and W-135) as compared with BOOSTRIX administered first. (nih.gov)
In subjects aged 19 to 64 years, lower levels for antibodies to filamentous hemagglutinin (FHA) and PRN were observed when BOOSTRIX was administered concomitantly with an inactivated influenza vaccine as compared with BOOSTRIX alone. (nih.gov)
The rapid screening for antibody levels in the healthy population, such as health care workers, is important for quickly assessing the number of susceptible individuals when a measles outbreak occurs. (gla.ac.uk)

In fact, as my colleague David Wallace-Wells has explained , should the most transmissible strains become dominant, herd immunity against COVID may become a mathematical impossibility. (nymag.com)

The C-strain antibody can be tested in pigs as early as 7 days post vaccination with the cELISA. (biomedcentral.com)
In this prospective two-center study, antispike 1 IgG and surrogate neutralizing antibodies were measured in 173 kidney transplant recipients and 166 healthy controls with different vaccination schedules. (lww.com)
In addition, different SARS-CoV-2 epitope antibodies from 135 vaccinated kidney transplant recipients were compared with antibodies in 25 matched healthy controls after second vaccination. (lww.com)

The proof-of-concept AMP studies demonstrated that a broadly neutralizing antibody (bnAb) called VRC01 was effective at preventing the acquisition of HIV strains to the 30% of strains that were sensitive to the bnAb. (medicalxpress.com)

The Covid-19 vaccine from Pfizer and BioNTech appears to effectively protect against mutant strains of the virus, new research suggests. (yorkshireeveningpost.co.uk)
An alternative anti-HIV strategy called Vectored ImmunoProphylaxis (VIP) designed by researchers at the Ragon Institute of MGH, MIT and Harvard and Massachusetts General Hospital (MGH) involves an adeno-associated viral (AAV) vector to deliver instructions to muscle cells to pump out antibodies that block the virus. (sciencedaily.com)
This has prompted discussion about whether or not there are several strains, if this has an impact on how easily the virus can infect a host, and whether or not this affects how many more people are likely to die. (medicalnewstoday.com)
If a particular genetic alteration changes the target of a drug or antibody that acts against the virus, those viral particles with the mutation will outgrow the ones that do not have it. (medicalnewstoday.com)
For this reason, researchers at MIT and other institutions are preparing a new strategy against the virus - a novel vaccine that, unlike those in use today, could potentially counteract all variants of the disease, having a property called "pan-variance" that could circumvent the need for a different booster shot every time a new strain comes into circulation. (mit.edu)
Furthermore, to confirm that the antibodies could protect animals from disease, the researchers infected mice with three different alphaviruses: Chikungunya, the closely related O'nyong'nyong virus, or the more distantly related Mayaro virus. (genengnews.com)
It was argued that the sequence information currently available was insufficient to provide consensus information across the full range of virus strains available. (who.int)
The Committee noted that supplies of hyperimmune globulin and neutralizing antibodies to the two infectious forms of variola virus were extremely limited. (who.int)
Access to live virus stocks would be needed during the initial stages of monoclonal antibody production or if, for example, phage display systems were to be developed. (who.int)

B- cells isolated from adults encode neutralizing antibodies , some with affinity for VP5*, that afford broad protection in mice . (bvsalud.org)
Given our ability to encode any desired antibody into these vectors, we may be able to produce effective preventive treatments against a wide range of infectious diseases from malaria to COVID-19," he says. (sciencedaily.com)

Lateral-flow antigen tests have been introduced, but they are used primarily as binary qualitative tests and report only binding between antibody and antigen rather than neutralization 7 . (nature.com)

History of Sabin attenuated poliovirus oral live vaccine strains. (who.int)
The ful data concerning the history of attenuated poliovirus strains developed by one of us (Sabin, 1965) for vaccine production do not appear in a single journal. (who.int)

Our findings demonstrate that a single intramuscular injection in mice of AAV encoding R1a-B6 fused to Fc fragments of different isotypes equipped either, with or without antibody dependent cellular cytotoxicity (ADCC) activity, was able to drive sustained high-level expression (0.5-1.1 mg/mL) in sera with no evidence of reduction for up to 6 months. (frontiersin.org)
Notably, even immunodeficient and older mice were protected by this method, suggesting that expression of a monoclonal antibody alone is sufficient to protect mice from illness. (caltech.edu)
Employing the two most potent broadly neutralizing antibodies, the researchers treated the infected mice and saw markedly reduced joint disease caused by any of the viruses. (genengnews.com)

The envelope glycoprotein E2 of C-strain CSFV was successfully expressed in insect cells by using Bac-to-Bac® Baculovirus Expression System. (biomedcentral.com)

Antibodies to V3 were subsequently identified as an additional correlate of protection, exerting immune pressure on viruses infecting individuals who had received the vaccine. (wikipedia.org)
The result is that numerous antibodies circulate to block any interaction between HIV and the CD4 receptor on immune cells, essentially shutting the door on HIV's entry into the cells. (sciencedaily.com)
B.1.617.2 (Delta) was first isolated in India and also shows some evidence of immune escape, specifically from neutralizing antibodies, but to a lesser degree than B.1.351 7 . (nature.com)

The hypercontagious U.K. variant is rapidly spreading through Florida , while a South African strain infamous for its resilience against antibodies has been spotted in California . (nymag.com)

The British research team extracted blood from individuals who had received either one or two doses of the Pfizer mRNA vaccine then introduced isolates of the U.K. (B117) and South African (B1.351) COVID variants into those blood samples and observed how the antibodies and T cells of the vaccinated held up in battle with the COVID mutants. (nymag.com)
This study measured seroprevalence of antibodies against poliovirus serotypes 1 to 3 (PV1, PV2 and PV3) in 7-month-old infants who had received at least 4 doses of trivalent oral polio vaccine. (who.int)

When injected into a patient, the AAV therapy (called AAV8-VRC07) enters muscle cells, where the genetic sequence is read and translated to produce large quantities of the broadly neutralizing antibody (called VRC07) that are pumped out of the cells and travel through the blood to seek out their target. (sciencedaily.com)

Here, we described a competitive ELISA (cELISA) developed with a neutralizing anti-E2 monoclonal antibody. (biomedcentral.com)

The findings prove that the platform we designed is capable of producing long-lived expression of an antibody from a single injection. (sciencedaily.com)

She proposed quantifying the antibodies directed at the second and third variable loops (V2 and V3) of the HIV envelope protein gp120, as observed in the Phase 3 RV144 clinical HIV vaccine trial, which involved 16,402 participants in Thailand. (wikipedia.org)
A small phase I clinical trial has tested an anti-HIV strategy involving an adeno-associated viral vector-based gene delivery system that instructs cells to pump out antibodies that block HIV. (sciencedaily.com)

This was the first isolation and description of a family of antibodies known for their extreme potency in neutralizing HIV. (wikipedia.org)

Over the past few years we have had frequent requests for the details such as isolation and attenuation and accordingly we felt that bringing the data together in the report below would be both helpful and informative to those involved in the production and control of poliovirus vaccine (oral) prepared from these strains. (who.int)
Using cluster sampling, 72 eligible infants were tested for antibody against the 3 poliovirus serotypes according to WHO guidelines. (who.int)
Only 63.9% of participants were seropositive for antibodies against all 3 poliovirus serotypes. (who.int)
Except for PV2, the seroprevalence of antibody against the other 2 poliovirus serotypes, especially PV3, was unsatisfactory. (who.int)
À l'aide d'un échantillonnage en grappes, 72 nourrissons éligibles ont fait l'objet de tests de dépistage des anticorps contre les trois sérotypes de poliovirus conformément aux lignes directrices de l'Organisation mondiale de la Santé. (who.int)
À l'exception du sérotype 2 du poliovirus, la séroprévalence des anticorps dirigés contre les deux autres sérotypes, en particulier le sérotype 3, n'était pas satisfaisante. (who.int)

Anatomy17

Organisms28

Diseases8

Chemicals and Drugs90

Analytical, Diagnostic and Therapeutic Techniques and Equipment54

Phenomena and Processes59

Disciplines and Occupations6

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Information Science4

Health Care15

Titers6

Monoclonal antibodies4

Presented a neutralizing monocl1

Omicron19

Efficacy8

Vaccines8

Assay8

Infection18

Broadly neutralizing H2

Variants of concern3

Responses8

Production of antibodies1

Convalescent2

Mutations4

Epitopes4

Titer3

Protective4

Bind4

Viral strains1

Influenza strains1

Wuhan1

Serum samples2

Gp412

Assays4

Neutralize3

Receptor-bindin1

Response13

Laboratory2

Prophylactic2

Potent neutrali1

Vaccine strain3

MAbs2

Levels4

Immunity1

Vaccination3

BnAb1

Virus9

Encode2

Antigen1

Sabin2

Mice3

Glycoprotein1

Immune3

Variant1

Doses2

VRC071

ELISA1

Findings1

Clinical2

Potency1

Poliovirus6