• Using an E. coli strain deficient with ALKB, we found that ALKBH6 complements ALKB deficiency and increases resistance after alkylating agent treatment. (frontiersin.org)
  • SHuffle is a mutant E.coli strain lacking the two reductases ( trxB and gor ) with an additional suppressor mutation ( ahpC ) which restores viability, allowing the formation of stable disulfide bonds in the cytoplasm. (neb.com)
  • Preliminary studies can be carried out in bacterial expression systems, using the strong T5 promoter, which is recognized by E. coli polymerase, and allows efficient expression of proteins in any E. coli strain. (qiagen.com)
  • Inserts encoding proteins of interest are cloned into appropriate constructs and transformed into a suitable E. coli strain for expression. (qiagen.com)
  • There is the T5 promoter/lac operator transcription-translation system for expression in E. coli, the p10 promoter for baculovirus-based expression in insect cells, and the CAG (CMV/actin/globin) promoter for expression in mammalian cells. (qiagen.com)
  • QIA express pQE vectors combine a powerful phage T5 promoter (recognized by E. coli RNA polymerase) with a double lac operator repression module to provide tightly regulated, high-level expression of recombinant proteins in E. coli . (qiagen.com)
  • The effect of Pluronic depended on the strain of the mouse and the type of the promoter used. (nebraska.edu)
  • Furthermore, P85 activated the expression of luciferase gene driven by CMV promoter, NFκB and p53 response elements. (nebraska.edu)
  • Overall, the promoter selectivity suggested that Pluronic induced transcriptional activation of gene expression by activating the p53 and NFκB signaling pathways. (nebraska.edu)
  • D : float, Sympy Symbol/Expr, str Diffusivity. (nvidia.com)
  • Q : float, Sympy Symbol/Expr, str The source term. (nvidia.com)
  • https://turbmodels.larc.nasa.gov/implementrans.html Parameters ========== nu : float, Sympy Symbol/Expr, str The kinematic viscosity. (nvidia.com)
  • rho : float, Sympy Symbol/Expr, str The density of the fluid. (nvidia.com)
  • Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. (sigmaaldrich.com)
  • RNA extracted from mock-infected and WNV-infected cells was assessed for differential expression of genes using Affymetrix microarray. (molvis.org)
  • Low-level WNV infection of hRPE cells induced expression of genes that are typically associated with the host cell response to virus infection. (molvis.org)
  • Improved access to bioinformatics tools enables scientists to process, manage, and store raw data, annotate sequences to define genes, and quantitate RNA sequence data to get insight into gene expression patterns. (genengnews.com)
  • Data from these define genetic and non-genetic variation, define a measure of heritability for expression of individual genes, and provide the basis for a new method of data reduction for genetical genomics. (biomedcentral.com)
  • Gene-expression patterns used to differentiate four type cause heart damage, and markable finding that genes cur- types of SRBCT. (lu.se)
  • [ 6 ] However, the gene mutations responsible for the different forms of CMT1 are clearly myelin genes. (medscape.com)
  • It has also been shown that phototrophy can be induced in some strains under laboratory conditions, implying that bacteria in the genus Dokdonia are not obligate heterotrophs but are mixotrophic. (wikipedia.org)
  • Two such examples are presented in this issue - a structure of the E. coli terminator protein Rho (Fan & Rees, 2020), and the structure of a cyanate hydratase from a Serratia strain of bacteria (Pederzoli et al . (iucr.org)
  • The green fluorescent protein (GFP) of the jellyfish Aequorea victoria has been widely used as a biomarker in eucaryotic and prokaryotic cells and has potential for developing predictive models for behavior of single strains of bacteria in naturally contaminated food and environmental systems. (usda.gov)
  • However, constitutive production of GFP in bacteria can result in reduced fitness in the form of slower growth. (usda.gov)
  • In this study, we adopted a shotgun proteomics strategy to characterize 15 foodborne strains of biogenic-amine-producing bacteria. (bvsalud.org)
  • Returns a list of strings that contain the given substring (case insensitive), allowing for regular expressions. (ucar.edu)
  • The string expression to be matched, with possible regex ("regular expressions") syntax included. (ucar.edu)
  • Unlike str_match_ic , regular expressions are allowed. (ucar.edu)
  • A full description of the syntax and capabilities of regular expressions is beyond the scope of this document. (ucar.edu)
  • See the Unix/POSIX man page for REGEX (7) or similar documentation for a complete explanation, noting that NCL's implementation uses the "modern" form of regular expressions. (ucar.edu)
  • It is important to note that most regular expression operations are available as module-level functions and methods on compiled regular expressions . (python.org)
  • if A and B are both regular expressions, then AB is also a regular expression. (python.org)
  • For details of the theory and implementation of regular expressions, consult the Friedl book [Frie09] , or almost any textbook about compiler construction. (python.org)
  • A brief explanation of the format of regular expressions follows. (python.org)
  • Regular expressions can contain both special and ordinary characters. (python.org)
  • Special characters either stand for classes of ordinary characters, or affect how the regular expressions around them are interpreted. (python.org)
  • The top level documentation about Perl regular expressions is found in perlre . (perl.org)
  • After explaining the role of the backslash, it lists all the sequences that have a special meaning in Perl regular expressions (in alphabetical order), then describes each of them. (perl.org)
  • Perfect Javascript Form Validation using Regular Expressions. (9lessons.info)
  • I have also found regular expressions to be very good for form validation. (9lessons.info)
  • The fastest way to diagnose blastomycosis is direct identification of the broad based budding yeast forms under microscopy. (medscape.com)
  • E. coli B strains are "naturally" deficient in the Lon protease which in K-12 strains serves to degrade misfolded proteins and to prevent some cell cycle-specific proteins from accumulating. (neb.com)
  • Expression host strains for pETBlue and pTriEx vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. (sigmaaldrich.com)
  • The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA. (sigmaaldrich.com)
  • These results indicated that the presence of the high copy plasmid introduced a fitness problem in E. coli K-12 in the form of slower growth and reduced cell yield that was independent of GFP production. (usda.gov)
  • Amphiphilic triblock copolymers of ethylene oxide and propylene oxide (Pluronic) significantly enhanced expression of plasmid DNA in the skeletal muscle. (nebraska.edu)
  • Novagen Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. (sigmaaldrich.com)
  • 2013). More interesting is the idea that every expression of a heterologous protein stresses the host in different ways, which can lead to the upregulation of different proteins, and thus a different protein background, even when using the same, well utilized expression system. (iucr.org)
  • Proteins that require disulfide bonds for their folding thus can be oxidized and form stable disulfide bonds within the cytoplasm. (neb.com)
  • The pQE TriSystem Vector allows for high-level expression of His-tagged proteins from a single vector containing three different expression systems. (qiagen.com)
  • Our preliminary study using 2D isolates were recovered from liq- new cases throughout the world cu- gel electrophoresis in drug sensitive/ uid nitrogen, and sub-cultured in taneous leishmaniasis (CL) remains resistant strains of L. tropica showed RPMI1640 medium (Gibco/BRL) a serious public health problem in that some proteins were differentially supplemented with 10% fetal bovine numerous countries [1,2]. (who.int)
  • Myelinating Schwann cells form a myelin sheath around a single axon and express high levels of myelin-related proteins and messenger RNA (mRNA). (medscape.com)
  • To further characterize pathways involved in naphthalene degradation in NaphS2, the draft genome was sequenced, and gene and protein expression examined. (elsevierpure.com)
  • Thus, P85 enhanced luciferase expression by 17 to 19-fold in immunocompetent C57Bl/6 and Balb/c mice, while no enhancement was observed with athymic Balb/c nu/nu mice. (nebraska.edu)
  • The OmpT protease resides at the surface of wild type E. coli in both K-12 and B strains, presumably helping the cells to derive amino acids from their external environment. (neb.com)
  • The drug functions instead as an angiostatic agent, inhibiting blood vessel growth by decreasing extracellular protease expression and inhibiting endothelial cell migration. (medscape.com)
  • Relevant functional pathways were determined, and strains were differentiated into hierarchical clusters. (bvsalud.org)
  • Oral azoles, usually itraconazole or amphotericin B, are first-line treatment for blastomycosis depending on the severity of the disease, its clinical form, and the host's immune status. (medscape.com)
  • Drug products combine active pharmaceutical ingredients with excipients in a final formulation for delivery to patients in liquid or lyophilized (freeze-dried) packaged forms - with the latter requiring reconstitution in the clinical setting. (bioprocessintl.com)
  • The properties of this strain that contribute to its usefulness as a protein expression strain are described below. (neb.com)
  • Thus DE3 strains may exhibit uninduced target protein expression. (neb.com)
  • The pQE-TriSystem vector contains CAG, T5, and p10 promoters that enable 6xHis-tagged protein expression in mammalian, E. coli , and baculovirus-infected insect cells, respectively (see figure pQE TriSystem. pQE TriSystem. "> pQE TriSystem ). (qiagen.com)
  • Vector pQE-TriSystem constructs can be transformed into E. coli , used as a shuttle vector for recombinant protein expression in insect cells, or transfected into mammalian cells. (qiagen.com)
  • Additionally, stress intensity factor and crack-mouth opening expressions are determined. (astm.org)
  • Additionally, SHuffle strains express the disulfide bond isomerase DsbC within the cytoplasm. (neb.com)
  • Furthermore, neural progenitor cells (those self-renewing cellular factories responsible for the development and maintenance of many of the cell types in the brain) were less able to form balls of cells called neurospheres -- a laboratory test that reflects the number and robustness of nerve stem cells in a culture. (sciencedaily.com)
  • In order to compare our strains, we ran fermentation trials in laboratory conditions with a standard wort for all the strains and standard temperature conditions (SafLager: 12°C for 48h then 14°C / SafAle: 20°C). We focused on the following parameters: Alcohol production, residual sugars, flocculation and fermentation kinetic. (fermentis.com)
  • Biofilm expression by the outbreak clone was greater than that of sporadic isolates (p ≤ 0.0005). (cdc.gov)
  • Recently, biofilm-forming potential was cited as a reason that patients with C. parapsilosis -infected catheters should have the device removed ( 17 ). (cdc.gov)
  • Methods and Results: Biofilm formation on plastic and cFn-coated surfaces by the wild-type M1 and M41 strains, and their isogenid sc/1-inactivated mutants, we compared spectrophotometrically following crystal violet staining. (cdc.gov)
  • Both WT strains formed significantly richer biofilm on cFn than on plastic, indicating that cell anchoring with ECM strengthens the overall biofilm structure. (cdc.gov)
  • complementation of Scl1 expression in the mutant restored biofilm to wild-type level. (cdc.gov)
  • Biofilm formed on nECM was comparable to that formed on commercial cFn and there was a statistically significant decrease in biofilm formed by both scl1 mutants as compared with the WT strains. (cdc.gov)
  • Adherence and protein secretion did not correlate with strain pathogenicity. (cdc.gov)
  • gene expression and secretion. (lu.se)
  • RESULTS: Draft genome sequencing, gene expression analysis, and proteomic analysis revealed that NaphS2 degrades naphthoyl-CoA in a manner analogous to benzoyl-CoA degradation. (elsevierpure.com)
  • This represents a major proteomic dataset of biogenic-amine-producing strains. (bvsalud.org)
  • Most information known about the genus is from strains of the type species, Dokdonia donghanensis. (wikipedia.org)
  • For isolation of strain from seawater, the standard dilution plating technique was used and the isolate is cultured on marine agar at 25 °C. Gram-negative, non-motile, non-spore-forming, slightly halophilic, rod or elongated rod-shaped with carotenoid pigments but no flexirubin-type pigments Cells are 1.5-25.0μm in length and 0.3-0.6μm in width. (wikipedia.org)
  • A 'contaminant' in structural biology is generally 'undesirable' rather than 'unwholesome', as very careful and thorough analysis is required to deconvolute the unexpected results when a contaminant progresses through all the involved process of expression, purification, crystallization and structure determination. (iucr.org)
  • Because of the presence of gas-forming organisms, subcutaneous air is classically described in necrotizing fasciitis. (medscape.com)
  • The use of recombinant inbred lines allows an estimate of the heritability of expression measured by individual probes. (biomedcentral.com)
  • Expression of the L1 protein using recombinant DNA technology produces noninfectious virus-like particles (VLPs). (cdc.gov)
  • The general characteristics of Dokdonia species include gram-negative, non-motile, aerobic, catalase- and oxidase-positive, non-spore-forming rods or elongated rods. (wikipedia.org)
  • Clinically obtained C. albicans isolates form biofilms ( 14 ), which may be important for sustaining infection. (cdc.gov)
  • among others, the strained caused by financial and housing instability might contribute to addiction. (basisonline.org)
  • Conversely, reducing Usp16 expression in skin and nerve-progenitor cells from people with Down syndrome allowed the cells, which usually proliferate slowly, to assume normal growth patterns. (sciencedaily.com)
  • Manipulating the tongue to form a cloverleaf shape, showcasing intricate patterns and precision. (cognovision.com)
  • The disruption of this pathway could generate a strain incapable of surviving a typical human immune response and would represent an attractive candidate as an attenuated growth vaccine. (plos.org)
  • If 'D' is a Sympy Symbol or Expression then this is substituted into the equation. (nvidia.com)
  • If 'rho' is a Sympy Symbol or Expression then this is substituted into the equation to allow for compressible Navier Stokes. (nvidia.com)
  • T7 Express strains do not carry the DE3 prophage and better tolerate an SOS response. (neb.com)
  • 0 gene-expression profiling to tinct subtypes of SRBCTs (Fig. 1). (lu.se)
  • Fruity driven strain, gives a high mouthfeel and body to the beer. (fermentis.com)
  • A numerically generated expression to determine crack length in a compact tension specimen from back-faced strain compliance is presented. (astm.org)
  • Riddell, W & Piascik, RS 1998, ' A back face strain compliance expression for the compact tension specimen ', NASA Technical Memorandum , no. 208453. (researchwithrowan.com)
  • Thus, complex expressions can easily be constructed from simpler primitive expressions like the ones described here. (python.org)
  • The only way for a client to construct expressions is using our handwritten 'smart constructor' functions with their carefully chosen types. (stanford.edu)
  • Clients cannot construct ill-formed expressions. (stanford.edu)
  • If expression in mammalian or insect cells is required - to obtain post-translational modifications, for example - the same construct can be used without the need for time-consuming subcloning procedures. (qiagen.com)
  • 4 lead to the next division of the Ḳerobot scheme, which is pervaded by verse-forms due to Eleazar Ḳalir ( c . 800), and presents, from internal evidence, some of the antique melody which Duran ( l.c. ) attributed to him. (jewishencyclopedia.com)
  • This is not surprising given the strong evidence for interaction between myelin and axon gene expression in development and after experimental nerve lesions. (medscape.com)
  • To validate the GFP strain of E. coli K-12 for predictive modeling studies, growth kinetics of the parent and GFP strain were compared at 10, 25 and 40 C on sterilized cooked chicken breast meat (sterilized chicken). (usda.gov)
  • function validate ( form ) { var name = form. (9lessons.info)
  • The Affymetrix software Microarray Suite 4.0 and 5.0 (MAS 4 and MAS 5) estimate expression from the average difference of PM and MM fluorescence. (biomedcentral.com)
  • The defects in stem cell growth and self-renewal observed by the researchers can be alleviated by reducing the expression of just one gene on chromosome 21, they found. (sciencedaily.com)
  • These expressions agree well with previously published results. (astm.org)
  • There are 10 classified species (D. aurantiaca, D. diaphoros, D. donghaensis, D. eikasta, D. flava, D. genika, D. lutea, D. pacifica, D. ponticola, and D. sinensis) and many unclassified strains under the Dokdonia genus based on the NCBI taxonomy database. (wikipedia.org)
  • In early childhood the factors contributing to the emotional strain are almost always found in a conflicting mother-child relationship, very often against the background of a general deficiency in family interaction. (unboundmedicine.com)
  • This module provides regular expression matching operations similar to those found in Perl. (python.org)
  • We have tested heritability-weighted averages to define expression of a transcript and found that these allow detection of more QTLs than previously described methods. (biomedcentral.com)
  • A reaction against the abrupt dogmatism of this position has led many others to an equally abrupt and equally dogmatic assertion of the laws of the lower forms of physical struggle and selection which explain or describe progress in lower animals as sufficient for all purposes of sociology. (marxists.org)
  • Scientists armed with whole genome sequence data can now ask questions about genome structure and organization that impact gene expression. (genengnews.com)
  • Critical to sub-classify SRBCTs, Khan et al .6 used to measure genome-wide gene expres- .com decisions in cancer therapeutics are to microarrays to monitor the expression sion for a variety of biological samples. (lu.se)
  • Matches the boundary conditions at an interface Parameters ========== T_1, T_2 : str Dependent variables to match the boundary conditions at the interface. (nvidia.com)
  • Diffusion equation Parameters ========== T : str The dependent variable. (nvidia.com)
  • Burmeister A, Bitzenhofer NL, Grünberger A, Binder D, Drepper T, Wiechert W. Microfluidic heterogeneity analysis of fluorescence-based gene expression of industrially relevant production strains on single-cell level. (uni-bielefeld.de)
  • Microfluidic heterogeneity analysis of fluorescence-based gene expression of industrially relevant production strains on single-cell level", Presented at the 18. (uni-bielefeld.de)
  • Several alternative methods have been proposed to combine multiple probe-specific values into a single expression estimate. (biomedcentral.com)
  • Although gfp expression was not induced during growth on sterilized chicken, maximum specific growth rate (umax) of the GFP strain was reduced (P (usda.gov)
  • We report on experimental determination of the strain and bandgap of InAsP in epitaxially grown InAsP-InP core-shell nanowires. (lu.se)
  • Public datasets and online software at WebQTL [ 10 , 13 ] allow free exploration of the characteristics of this form of analysis [ 14 ]. (biomedcentral.com)
  • ABSTRACT In order to define the protein expressional changes related to the process of meglumine antimoniate resistance in anthroponotic cutaneous leishmaniasis (CL), we performed a comparative proteomics analysis on sensitive and resistant strains of Leishmania tropica isolated from Iranian CL patients. (who.int)
  • Red not have the tumor-specific ex- with these dangerous drugs, rhab- represents relatively high expression level, whereas green pression pattern. (lu.se)
  • Representative σ 66 -dependent gene transcription was repressed in the absence of rsbV1 or upon increased expression of RsbW, and increased upon elevated expression of RsbV1. (plos.org)
  • One note is the use of the optional marker: Given the condition '~foo', both '~foo' and 'foo' match the expression. (go.dev)
  • Note that str_match_ic_regex is case INSENSITIVE . (ucar.edu)
  • Use str_match_regex if you need case sensitivity. (ucar.edu)
  • We determine the predominantly uniaxial strains formed in the core-shell nanowires along the nanowire growth axis and demonstrate that the strains can be. (lu.se)
  • Beauty is a quality constituting the nonutilitarian value of a form, inhering in it as a subtle and hazardous union of the quantitative and qualitative elements, and discovered with increasing interest and adherence of the mind. (encyclopedia.com)
  • Note that these strains are not recommended for use with pET, pACYCDuet, pCDF, pRSF, pCOLADuet or pETcoco vectors. (sigmaaldrich.com)
  • Little is known about the pathogenic potential of these strains. (frontiersin.org)
  • If the sublime characterizes a structure (of meaning), the unexpressed potential seems, by the natural movement of the mind, to be multiplied, while the actual (expression) seems to advance toward relative annihilation. (encyclopedia.com)
  • On the other hand, if grace qualifies the form, the actual expression - casually and without strain - exceeds the expectation of already superior expression, and inference is drawn of potential still unactualized. (encyclopedia.com)
  • Recent advances in genetic manipulation of Chlamydia were employed to inactivate rsbV1 , as well as to increase the expression levels of rsbW or rsbV1 , in vivo . (plos.org)
  • A function defined by a function expression inherits the current scope. (stackoverflow.com)
  • Amy Radunz, a nutritionist from the department of animal sciences at the University of Wisconsin in River Falls, has partnered with molecular biologist Hasan Khatib, an associate professor at University of Wisconsin-Madison, to ask how differences in maternal diet during pregnancy induce gene expression and DNA methylation changes in fetal tissues in sheep. (genengnews.com)