• The investigators used gene editing techniques to engineer A. vinelandii to produce ammonia at a constant level, regardless of environmental conditions surrounding the bacteria, and to excrete it at concentrations high enough to effectively fertilize crops. (phys.org)
  • The proprietary synbiotic formulation contains 25 clinically proven and scientifically studied bacteria strains, which can help maintain gut health, coupled with psyllium husk, and can act as the prebiotic food for healthy bacteria. (gudhealthy.com)
  • It contains 11 strains of bacteria and microfungi that live in symbiosis and form a living system around the plant root and in the substrate. (romb.bio)
  • The molecular structures of P3HB, P3HB3HV, and P3HB3HH, and a transmission electron micrograph of a thin section of Azotobacter vinelandii bacteria containing P3HB granules (white inclusions), are shown below. (polymerexpert.biz)
  • This suggests that this ORF is functional and is essential for the vanadium-dependent alternative pathway of nitrogen fixation in A. vinelandii. (syr.edu)
  • Raina, R , Bageshwar, UK & Das, HK 1993, ' The ORF encoding a putative ferredoxin-like protein downstream of the vnfH gene in Azotobacter vinelandii is involved in the vanadium-dependent alternative pathway of nitrogen fixation ', MGG Molecular & General Genetics , vol. 236, no. 2-3, pp. 459-462. (syr.edu)
  • Das, H. K. / The ORF encoding a putative ferredoxin-like protein downstream of the vnfH gene in Azotobacter vinelandii is involved in the vanadium-dependent alternative pathway of nitrogen fixation . (syr.edu)
  • Researchers from Washington State University have engineered strains of the ubiquitous, nitrogen-fixing soil bacterium Azotobacter vinelandii to produce ammonia and excrete it at high concentrations, transferring it into crop plants in lieu of conventional chemical fertilizers. (phys.org)
  • The growth of the Na + -dependent soil bacterium Azotobacter salinestris strain 184 was inhibited only 36% by 50 μ m -carbonyl cyanide m -chlorophenylhydrazone (CCCP) at alkaline pH, whereas other species of this genus were inhibited 80-90% under the same conditions. (microbiologyresearch.org)
  • Bacillus anthracis str. (up.ac.za)
  • Bacillus thuringiensis serovar konkukian str. (up.ac.za)
  • The first ORF was identified as fixX on the basis of sequence homology with fixX from several Rhizobium and Bradyrhizobium strains. (hal.science)
  • We sequenced and constructed the complete genome of an environmental strain CR1 of P. aeruginosa and performed the comparative genomic analysis. (frontiersin.org)
  • We identified eight new regions of genomic plasticity and a plasmid pCR1 with a VirB/D4 complex followed by trimeric auto-transporter that can induce virulence phenotype in the genome of strain CR1. (frontiersin.org)
  • The use of gene editing techniques in lieu of inserting transgenes into the A. vinelandii genome allowed regulatory requirements to be avoided that would have made the development process slower, and more difficult and expensive. (phys.org)
  • This construct was mobilized into A. vinelandii CA12 (Δ nifHDK) strain by conjugation and the mutation was introduced into the genome by marker exchange. (syr.edu)
  • One of the key enzymes for this pathway (encoded by thiF of most organisms) is not present among the proteins predicted by the genome sequence of A. vinelandii . (vt.edu)
  • 2009. The genome sequence of Azotobacter vinelandii , an obligate aerobe specialized to support diverse anaerobic metabolic processes. (vt.edu)
  • Florence Mus et al, Genetic determinants of ammonium excretion in nifL mutants of Azotobacter vinelandii, Applied and Environmental Microbiology (2022). (phys.org)
  • Three of the mutants were deficient in diaminopimelate decarboxylase and accumulated meso- and ll -diaminopimelic acid (DAP) but otherwise were indistinguishable from the parent strain. (microbiologyresearch.org)
  • Further work on purification and characterization of these proteins is needed, along with characterization of mutant strains deficient in the respective genes. (vt.edu)
  • Crystal Growth and Characterization of Residual Bacterioferritin in Partially Purified Nitrogenase CrFe Protein Solution from a Mutant UW3 of Azotobacter vinelandii [J]. J Integr Plant Biol. (jipb.net)
  • Our work helps provide a more complete, fundamental understanding of the factors that underpin gene expression in a model nitrogen fixing microorganism and defines the biochemistry that brings about ammonia excretion in A. vinelandii," said Mus. (phys.org)
  • strain JS443, another two-component monooxygenase gene NpdA1A2 has been identified [ 4 ]. (biomedcentral.com)
  • AE24 ACIN2 Acinetobacter venetianus Aven Acrocarpospora corrugata Acor Acrocarpospora pleiomorpha Aple Actinoallomurus spadix Aspa Actinoalloteichus cyanogriseus Acya Actinobacillus capsulatus Acap1 Actinobacillus equuli Aequ Actinobacillus hominis Ahom Actinobacillus indolicus Aind Actinobacillus lignieresii Alig Actinobacillus minor Amin1 Actinobacillus muris Amur Actinobacillus pleuropneumoniae Aple1 Actinobacillus pleuropneumoniae serovar 1 str. (uni-freiburg.de)
  • 4074 Aple1_A Actinobacillus pleuropneumoniae serovar 3 str. (uni-freiburg.de)
  • JL03 Aple1_C Actinobacillus pleuropneumoniae serovar 5b str. (uni-freiburg.de)
  • L20 Aple1_B Actinobacillus pleuropneumoniae serovar 7 str. (uni-freiburg.de)
  • enterica serovar Schwarzengrund str. (weizmann.ac.il)
  • enterica serovar Typhi str. (up.ac.za)
  • Simple and Effective Method for development of longer shelf life liquid formulation with Azospirillum brasilense strain Asp-7. (abfindia.org)
  • From the scientific point of view biofertilizer is an individual microorganism exerting plant growth promotion properties, but in the agronomical context this term pertain to product composed of beneficial strain(s), which are useful regarding nutrient mobilization, included in a carrier, possessing features that allow its storage at the time specified by the producer, and ready to effective application to the soil or plant. (nubco.org)
  • Current focus is on the nitrogen fixing, obligate aerobe, Azotobacter vinelandii , whose natural habitat is the soil. (vt.edu)
  • The nitrogenase holoenzyme of A. vinelandii has been characterised by X-ray crystallography in both ADP tetrafluoroaluminate-bound and MgATP-bound states. (wikipedia.org)
  • A. vinelandii has three genes predicted to encode sulfur dioxygenase. (vt.edu)
  • strain PN1, a two component PNP monooxygenase NpsA1A2 was found to catalyze PNP to both HQ and BT in the presence of ascorbic acid as a reducing reagent. (biomedcentral.com)
  • Analysis of an acid over-producing strain showed that Rb + appeared to be an uncompetitive inhibitor of Na + -dependent growth and in competition with Na + as a promoter of acidification. (microbiologyresearch.org)
  • Mutant strains deficient in each of these genes will be constructed, and phenotypes will be assessed. (vt.edu)
  • A. vinelandii is no exception, where 12 rhodanese-containing proteins are predicted. (vt.edu)
  • Due to this characteristic, A. vinelandii exhibits a high respiratory activity, especially when exposed to high oxygen concentrations [ 4 ]. (biomedcentral.com)
  • Virulence genotype analysis revealed that strain CR1 lacked hemolytic phospholipase C and D, three genes for LPS biosynthesis and had reduced antibiotic resistance genes when compared with clinical strains. (frontiersin.org)
  • Candidatus Accumulibacter phosphatis clade IIA str. (sjtu.edu.cn)
  • Metabolism of chloramphenicol in strains containing R1 B1 was greater than in those with R 1a. (microbiologyresearch.org)
  • Azotobacter vinelandii is Gram-negative diazotroph that can fix nitrogen while grown aerobically. (wikipedia.org)
  • Genetically engineered strains can produce significantly higher amounts of ammonia. (wikipedia.org)
  • But they expect to be able to design different groups of A.vinelandii to produce ammonia at different rates to fit the needs of different species of crop plants. (phys.org)
  • A powerful blend of all natural ingredients and 29 different strains of mycorrhizae and beneficial microbes. (sweetgrasshydroponics.com)
  • A powerful blend of all natural ingredients & 29 different strains of mycorrhizae & beneficial microbes designed for today's demanding "High Performance Gardening. (monstergardens.com)
  • A. vinelandii can contain up to 80 chromosome copies per cell. (wikipedia.org)
  • To our knowledge, there have been no studies about the relationship between NADH and NADPH levels and the metabolic behavior of A. vinelandii . (biomedcentral.com)
  • The majority of the ATPase activity on everted membrane vesicles prepared from strain 184 grown at alkaline pH was sensitive to azide and N , N ′-dicyclohexylcarbodiimide (DCCD), but ATPase was less sensitive to these inhibitors when Na + was present. (microbiologyresearch.org)
  • Drought tolerant Azotobacter strain azt-7 effect on growth and development of okra seedlings under drought stress.International Journal of Current Advanced Research.7: 8920-8926. (abfindia.org)
  • Study on the Structure and Genetic Polymorphism of Embryo Sac in Polyembryonic Rice Strain AP Ⅳ [J]. J Integr Plant Biol. (jipb.net)
  • Changes of ploidy during the Azotobacter vinelandii growth cycle" (PDF). (wikipedia.org)
  • 10 products with undefined mechanism (apart from "plant growth promotion") consisting of 1-30 strains of PGPR. (nubco.org)
  • Growth of strain 184 at alkaline pH was inhibited 66% by 50 μ m -monensin and 100% by monensin plus CCCP. (microbiologyresearch.org)
  • Rb + was a more complex competitor and affected the final yield and the growth rate of strain 184. (microbiologyresearch.org)
  • Strain indexes such as relative growth rate etc. were determined. (jipb.net)
  • Strain Responses in Na2CO3-Stressed Leymus chinensis Seedlings and Their Mathematical Analysis [J]. J Integr Plant Biol. (jipb.net)