AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceHealth Care
Escherichia coliEscherichia coli ProteinsBacterial ProteinsEscherichia coli InfectionsMolecular Sequence DataGenes, BacterialAmino Acid SequenceBase SequencePlasmidsEscherichia coli O157MutationCloning, MolecularDNA, BacterialSequence Homology, Amino AcidAdenomatous Polyposis Coli ProteinKineticsBacteriophage lambdaBacterial Outer Membrane ProteinsEscherichia coli K12Gene Expression Regulation, BacterialRecombinant ProteinsAdenosine TriphosphatasesMembrane ProteinsTemperatureRibosomal ProteinsBinding SitesElectrophoresis, Polyacrylamide GelAdhesins, Escherichia coliProtein BindingModels, MolecularOperonRNA, BacterialMolecular WeightProtein ConformationHeat-Shock ProteinsChromosomes, BacterialColiphagesGenetic Complementation TestGenesRestriction MappingDNA-Binding ProteinsSpecies SpecificityAnti-Bacterial AgentsCulture MediaSequence Analysis, DNASubstrate SpecificityBacterial ToxinsTranscription, GeneticBacterial AdhesionRecombinant Fusion ProteinsEnterotoxigenic Escherichia coliConjugation, GeneticShiga-Toxigenic Escherichia coliDrug Resistance, MicrobialFimbriae, BacterialSequence AlignmentCarrier ProteinsVirulenceEnteropathogenic Escherichia coliEscherichiaFecesMutagenesis, Site-DirectedChromosome MappingDiarrheaEnterohemorrhagic Escherichia coliPolymerase Chain ReactionDNA PrimersMicrobial Sensitivity TestsHot TemperatureUropathogenic Escherichia coliEnterotoxinsbeta-GalactosidaseHydrogen-Ion ConcentrationDNA Transposable ElementsMaltose-Binding ProteinsChloramphenicolPhenotypeFimbriae ProteinsEscherichia coli VaccinesIntegration Host FactorsTransduction, Genetic
BacteriaShigellaCFT073BL21EnterohemorrhagicMutationO157:H7GenesIsolatesSolubleCharacterizationOuter membrane proteinEukaryoticGroup of proteinsSequenceStaphylococcusSusceptibilityBiologicalGenomePathogenicATCCPseudomonasAtypicalSubstrBacillusProteaseNatively disordered regionsReagentsKinasesBacterialCysteinePurificationMutationsBacteroidesMutantHemeAssayMRNAInfectionKinaseSpeciesResistanceExpressionHomologousPenicillinDerivativesRevealProteomePeptidesPhenotypicPredictionIsolate
Bacteria7
  • Bacteriocins are protein antibiotics produced by strains of bacteria that are active against related bacteria. (nottingham.ac.uk)
  • Tetracyclines inhibit protein synthesis and, therefore, bacterial growth by binding with 30S and possibly 50S ribosomal subunits of susceptible bacteria. (medscape.com)
  • Enterotoxins, generated by some bacteria (ie, enterotoxigenic Escherichia coli, Vibrio cholera ) act directly on secretory mechanisms and produce a typical, copious watery (rice water) diarrhea. (medscape.com)
  • Cytotoxin production by other bacteria (ie, Shigella dysenteriae, Vibrio parahaemolyticus, Clostridium difficile, enterohemorrhagic E coli ) results in mucosal cell destruction that leads to bloody stools with inflammatory cells. (medscape.com)
  • Unlike the atypical form, the typical form is caused by infection with certain strains of Escherichia coli bacteria that produce toxic substances called Shiga-like toxins. (medlineplus.gov)
  • This system is a group of proteins that work together to destroy foreign invaders (such as bacteria and viruses), trigger inflammation, and remove debris from cells and tissues. (medlineplus.gov)
  • Infection by Escherichia coli O157:H7 and Other Enterohemorrhagic E. coli (EHEC) The gram-negative bacteria Escherichia coli O157:H7 and other enterohemorrhagic E. coli (EHEC) typically cause acute bloody diarrhea, which may lead to hemolytic-uremic syndrome. (msdmanuals.com)
Shigella4
  • Thus, herein we successfully obtained a recombinant UspF protein from aEPEC, which is a α/β, ATP-binding protein involved in stress response, with comparable protein production among the four studied strains, but showing noteworthy differences when cultivated in different stress conditions, also present in other enterobacterial species, such as Shigella sonnei and Citrobacter freundii. (utmb.edu)
  • Enterocyte invasion is the preferred method by which microbes such as Shigella and Campylobacter organisms and enteroinvasive E coli cause destruction and inflammatory diarrhea. (medscape.com)
  • Ampicillin is a second-generation penicillin that is active against many strains of Escherichia coli, Proteus mirabilis, Salmonella, Shigella, and Haemophilus influenzae. (medscape.com)
  • or some strains of Shigella dysenteriae ). (msdmanuals.com)
CFT0731
BL212
  • Escherichia coli strains TG1 and BL21 (DE3) were grown at 37°C in LB medium. (hindawi.com)
  • Fragmen gen xynA disubkloning menggunakan plasmid pET-21b dan diekspresikan pada Escherichia coli BL21 (DE3) pLys. (biotrop.org)
Enterohemorrhagic2
  • The crystal structure of a C-terminal domain of enterohemorrhagic Escherichia coli type III effector NleH2 has been determined to 2.6 Å resolution. (northwestern.edu)
  • The majority of the hypothetical proteins were homologue products of genes identified in the genome of enterohemorrhagic E. coli. (utmb.edu)
Mutation2
  • The reason for the observed defective assembly in E. coli is probably not some unidentified mutation in the plasmid DNA because the same plasmid preparation complemented a B. subtilis sdhCAB deletion strain [7]. (lu.se)
  • Complement dysregulation is usually from a mutation in genes controlling complement proteins or factors but sometimes from acquired autoantibodies to certain complement factors. (msdmanuals.com)
O157:H72
Genes4
  • In addition to these promotion-flowering genes, TERMINATING FLOWER ( TMF ) encodes an ALOG family protein and affects inflorescence organization in tomato. (nature.com)
  • The beta-galactosidase activity in strains producing a PulG-LacZ hybrid protein increased substantially when the dsbA, dsbB, or all sec genes tested except secB were compromised by mutations. (pasteur.fr)
  • Unprocessed PrePulG* was detected in strains carrying mutations in secA, secY, secE, and secD genes, including some novel alleles of secY and secD. (pasteur.fr)
  • The genes associated with atypical hemolytic-uremic syndrome provide instructions for making proteins involved in a part of the body's immune response known as the complement system. (medlineplus.gov)
Isolates1
Soluble2
  • The recombinant protein was expressed and purified from Escherichia coli in the soluble form, and biochemically characterized. (us.es)
  • If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)
Characterization2
  • The molecular approach of using Geneious Prime software and protein prediction data base for characterization of bacteriocin is novel and predicts " KSSAYSLQMGATAIKQVKKLFKKWGW " as peptide responsible for antimicrobial activity. (researchsquare.com)
  • We show that these microarrays are suitable for the high throughput characterization of the recognition capabilities of monoclonal antibodies, carbohydrate-binding modules, and other oligosaccharide-binding proteins of biological significance and also that they have potential for the characterization of carbohydrate-active enzymes. (megazyme.com)
Outer membrane protein2
  • Ertapenem resistance may be explained by a defect in the outer membrane protein and production of extended-spectrum β-lactamase CTX-M-2. (cdc.gov)
  • Modulations in lipid A and phospholipid biosynthesis pathways influence outer membrane protein assembly in Escherichia coli K-12. (wikigenes.org)
Eukaryotic3
  • and it has been within many diseases especially cancer rendering it an interesting focus on inside CX-6258 manufacture the druggable category of eukaryotic proteins kinases (Pagano et al. (immune-source.com)
  • The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. (antikoerper-online.de)
  • The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. (antikoerper-online.de)
Group of proteins2
  • Furthermore, our results confirm that the Usp protein superfamily encompasses a conserved group of proteins involved in stress resistance in aEPEC and other Enterobacteriaceae. (utmb.edu)
  • Known as "destabilising motifs", these sequences attract the attention of a group of proteins called Ccr4-Not. (elifesciences.org)
Sequence4
  • PASS2 version 6: a database of structure-based sequence alignments of protein domain superfamilies in accordance with SCOPe. (ncbs.res.in)
  • RStrucFam: a web server to associate structure and cognate RNA for RNA-binding proteins from sequence information. (ncbs.res.in)
  • It has a signal sequence-like N-terminal segment that, according to studies with green and red fluorescent protein chimeras, anchors unassembled PulG in the inner membrane. (pasteur.fr)
  • Beta-ketoacyl-ACP synthase I of Escherichia coli: nucleotide sequence of the fabB gene and identification of the cerulenin binding residue. (genebiosystems.com)
Staphylococcus2
  • Protein A is a highly stable cell surface receptor produced by several strains of Staphylococcus aureus. (sigmaaldrich.com)
  • 10 kDa in size with 37-48 amino acids that inhibit growth of food spoilage organisms such as Listeria monocytogenes, Bacillus cereus, Clostridium perfringens, Staphylococcus aureus and E. coli (Rodrigues et al. (researchsquare.com)
Susceptibility1
  • We describe the clinical and microbiologic features associated with an ertapenem-resistant E. coli isolate that had reduced susceptibility to imipenem after in vivo treatment with imipenem/cilastatin and provide a detailed molecular analysis of the antimicrobial drug resistance mechanisms. (cdc.gov)
Biological3
  • How proteins are translocated across membrane barriers is a key issue in the biological and biomedical sciences. (nottingham.ac.uk)
  • Structural evidence that colicin A protein binds to a novel binding site of TolA protein in Escherichia coli periplasm Journal of Biological Chemistry. (nottingham.ac.uk)
  • These studies provide the IRG with insight into how membrane strain is connected to the organization of phospholipids and the biological function of the resulting material. (wisc.edu)
Genome2
  • Genome-wide survey of putative RNA-binding proteins encoded in the human proteome. (ncbs.res.in)
  • 3, 137-155 (1996) REFERENCE 9 AUTHORS Fujita,N., Mori,H., Yura,T. and Ishihama,A. TITLE Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region JOURNAL Nucleic Acids Res. (nig.ac.jp)
Pathogenic1
ATCC4
  • There has been much research on AmtR structure, functions, and regulons in the type strain C. glutamicum ATCC 13032, but little research in other C. glutamicum strains. (frontiersin.org)
  • The RS_01910, RS_01915, RS_15995, and RS_16000 are not present in the type strain C. glutamicum ATCC 13032. (frontiersin.org)
  • Real-time quantitative PCR (RT-qPCR) analysis and sfGFP (superfolder green fluorescent protein) analysis reveal that AmtR directly and negatively regulates the transcription and expression of crn A, cre T, csh A, and hyu B. A growth test shows that C. glutamicum ATCC 14067 can use creatine or creatinine as a sole nitrogen source. (frontiersin.org)
  • The research on transcription factors in C. glutamicum was mainly focused on the type strain C. glutamicum ATCC 13032. (frontiersin.org)
Pseudomonas1
  • polyamine ABC transporter, ATP-binding protein [Pseudomonas syringae pv. (cornell.edu)
Atypical1
  • The regulatory proteins associated with atypical hemolytic-uremic syndrome protect healthy cells by preventing activation of the complement system when it is not needed. (medlineplus.gov)
Substr1
  • Escherichia coli K-12 substr. (lbl.gov)
Bacillus2
  • Identification of an acetyl esterase in the supernatant of the environmental strain Bacillus sp. (us.es)
  • Why is Bacillus subtilis respiratory Complex II not assembled when produced in Escherichia coli? (lu.se)
Protease2
  • Balanced biosynthesis of major membrane components through regulated degradation of the committed enzyme of lipid A biosynthesis by the AAA protease FtsH (HflB) in Escherichia coli. (wikigenes.org)
  • The GST-fused proteins was cleaved by PreScission protease (GE Health care) in the column in cleavage buffer at 277?K for 16?h. (immune-source.com)
Natively disordered regions1
  • 3) Protein recruitment by natively disordered regions (NDRs). (nottingham.ac.uk)
Reagents1
  • Once coupled to protein, these neoglycoconjugates are versatile reagents that can be printed as microarrays onto a variety of slide types and membranes. (megazyme.com)
Kinases2
  • The structure resembles those of protein kinases featuring the catalytic, activation, and glycine-rich loop motifs and ATP-binding site. (northwestern.edu)
  • Proteins kinase CK2 (previously called casein kinase 2) is a pivotal and ubiquitously expressed member of the protein CX-6258 manufacture kinase CMGC subfamily which includes cyclin-dependent kinases (CDKs) mitogen-activated protein kinases (MAPKs) glycogen synthase kinases (GSKs) and CDK-like kinases (CLKs). (immune-source.com)
Bacterial7
  • The Tat system is a recently discovered protein export pathway that serves to translocate folded proteins, often containing redox cofactors, across the bacterial cytoplasmic membrane. (ox.ac.uk)
  • CusabioProtein Description: Full Length Alternative Name (s) : Bacterial nodulin-like intrinsic proteinGene Names: AQPZResearch Areas: MicrobiologySpecies: Escherichia coli. (joplink.net)
  • NDRs are functionally important in proteins throughout the three kingdoms of life as well as being implicated in cancer, amyloid diseases and bacterial pathogenesis. (nottingham.ac.uk)
  • Contamination by bacterial viruses is one of the most difficult problems to evaluate in the modern biotechnology industry, especially in the productions of recombinant proteins that are based on Escherichia coli strains. (sld.cu)
  • The approach allows efficient incorporation of heme analogs using a widely available bacterial strain and offers an attractive alternative to present reconstitution methods that subject proteins to harsh, denaturing conditions. (nih.gov)
  • Nickel ABC transporter, substrate-binding protein NikA / Solute-binding protein family 5, conserved site / Bacterial extracellular solute-binding proteins, family 5 signature. (pdbj.org)
  • Carbapenems inhibit bacterial cell wall synthesis by binding to penicillin-binding proteins. (medscape.com)
Cysteine1
Purification2
  • 2.2 Protein expression and purification Escherichia coli strain HMS174 (DE3) cells (Novagen) were transformed with pGEX-hCK2?2. (immune-source.com)
  • In addition, protein A may also be immobilized on a solid supports like agarose or acrylic beads for the purification of polyclonal or monoclonal immunoglobulins. (sigmaaldrich.com)
Mutations3
  • Both phenotypes are similar to those displayed by strains carrying point mutations in the lpxC (envA) gene. (ox.ac.uk)
  • Some parental strains had mutations initially, which were retained in the mutants. (cdc.gov)
  • CL-deficient strains of E. coli displayed elevated rates of accumulating spontaneous mutations compared to wild-type E. coli, which is consistent with the unregulated activation of RecA. (wisc.edu)
Bacteroides1
Mutant7
  • Here we report that tat strains are associated with a mutant cell septation phenotype, where chains of up to 10 cells are evident. (ox.ac.uk)
  • Mutant strains are also hypersensitive to hydrophobic drugs and to lysis by lysozyme in the absence of EDTA, and they leak periplasmic enzymes, characteristics that are consistent with an outer membrane defect. (ox.ac.uk)
  • The Escherichia coli dnaN159 allele encodes a mutant form of the β-sliding clamp (β159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. (asm.org)
  • These phenotypes appear to result, at least in part, from impaired interactions of the mutant β159 clamp protein with the replicative DNA polymerase, Pol III ( 38 ). (asm.org)
  • In comparison, a cre T deletion mutant strain is able to grow on creatinine but loses the ability to grow on creatine. (frontiersin.org)
  • However, the E. coli cells did not show increased succinate dehydrogenase activity nor did the operon complement a sdhCDAB defective E. coli mutant [7]. (lu.se)
  • The slight difference in electrophoretic mobility of normal Fp and that produced in E. coli could be due to the absence of covalently FAD but could have another explanation because this difference was not observed for some mutant Fp without covalently bound FAD [3]. (lu.se)
Heme2
  • Heme exporter protein A (Cytochrome c-type biogenesis ATP-binding protein ccmA) [Shigell. (cornell.edu)
  • The membrane anchor of SQR in mammalian mitochondria and proteobacteria, such as Escherichia coli , consists of two polypeptides (SdhC and SdhD) and contains one heme group. (lu.se)
Assay1
  • Here, we have developed and characterized a droplet digital PCR single-nucleotide discrimination assay to detect chimerism among common albino and non-albino mouse strains. (stanford.edu)
MRNA3
  • When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
  • The more mRNA copies it makes, the more protein it can produce. (elifesciences.org)
  • A simple way to control protein production is to raise or lower the number of these mRNA messages, and living cells have lots of ways to make this happen. (elifesciences.org)
Infection2
  • Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)
  • E. coli serotype O5 factor for the development of urinary tract was the most prevalent (29.3%) followed by infection (UTI) [ 1-3 ]. (who.int)
Kinase2
  • Escherichia coli osmosensor EnvZ is a protein histidine kinase that plays a central role in osmoregulation, a cellular adaptation process involving the His-Asp phosphorelay signal transduction system. (rcsb.org)
  • is a constitutively active protein kinase and is further fully activated by association with CK2? (immune-source.com)
Species1
  • This protein is capable of binding to the Fc portion of immunoglobulins, especially IgGs, from a large number of species. (sigmaaldrich.com)
Resistance3
  • Spread of CTX-M type ESBLs, especially in Escherichia coli, may provide a favorable background for selection of carbapenem resistance. (cdc.gov)
  • Resistance to the recently introduced ertapenem has not been reported in E. coli associated with a CTX-M-type enzyme. (cdc.gov)
  • Naoise McGarry, Stephen Smith, Escherichia coli serum resistance : roles for cell number and Haemolysin. (tcd.ie)
Expression1
  • Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies. (antikoerper-online.de)
Homologous2
  • this protein homologous to HlyB, which participates in the. (cornell.edu)
  • RecA is a widely conserved cytoplasmic protein that performs a range of functions in Escherichia coli, including: DNA repair, homologous recombination, and induction of the SOS pathway. (wisc.edu)
Penicillin1
  • Serogroup A strains are by the disc diffusion method and in- ~10% in treated cases but varies widely homogeneous and have the same an- cluded penicillin G, amoxicillin, cefo- depending on the clinical presentation tigenic formula, A:4:P1.9 ( 2 , 10 , 11 , 14 ), taxime, chloramphenicol and rifampin. (who.int)
Derivatives1
  • We analyzed the early steps of PulG inner membrane targeting and insertion in Escherichia coli derivatives defective in different protein targeting and export factors. (pasteur.fr)
Reveal1
  • Crystal structures of the Toll/Interleukin-1 receptor (TIR) domains from the Brucella protein TcpB and host adaptor TIRAP reveal mechanisms of molecular mimicry. (umm.de)
Proteome1
  • A quarter of the E. coli proteome is extracytoplasmic, targeted to a final destination by short, canonical N-terminal sequences that for the most part direct them either to the general secretory or Tat pathway machines. (nottingham.ac.uk)
Peptides3
  • We assessed changes in the gut microbiota, intestinal permeability, gut peptides, intestinal epithelial tight-junction proteins ZO-1 and occludin (qPCR and immunohistochemistry), hepatic and systemic inflammation. (bmj.com)
  • Water 2H and 17O spin relaxation is used to study water dynamics in the hydration layers of two small peptides, two globular proteins and in living cells of two microorganisms. (lu.se)
  • This distribution comprises a small fraction of protein-specific hydration sites, where water rotation is strongly retarded, and a dominant fraction of generic hydration sites, where water rotation is as fast as in the hydration shells of small peptides. (lu.se)
Phenotypic1
  • These strains have apparent differences in several phenotypic characteristics ( Yang and Yang, 2017 ). (frontiersin.org)
Prediction1
  • 2020. A knowledge-driven protocol for prediction of proteins of interest with an emphasis on biosynthetic pathways. . (ncbs.res.in)
Isolate2
  • An ertapenem-resistant Escherichia coli isolate was recovered from peritoneal fluid in a patient who had been treated with imipenem/cilastatin for 10 days. (cdc.gov)
  • For this purpose we used an environmental bacteriophage isolate and the LE392 sensitive strain and bases of the firms OXOID, BioCen and Biolife. (sld.cu)