Loading...
  • GTPase
  • Ras is a membrane-bound small GTPase that plays a pivotal role in transducing responses to diverse extracellular signals that impact various cellular processes, prominently cell proliferation, differentiation, apoptosis ( 1 ). (frontiersin.org)
  • The physiological importance of Ras' GTPase activity was recognized in the late 80s through the detection and biochemical characterization of GTPase impairing Ras mutations commonly found in various human tumor tissues ( 6 ). (frontiersin.org)
  • The GTPase family contains sub families such as the RAS super family which is critical to cell signaling. (thermofisher.com)
  • Ras GTPase family containing H-Ras,N-Ras and K-Ras4A/4B. (umbc.edu)
  • Rheb and TSC2 comprise a unique pair of GTPase and GAP, because Rheb has high basal GTP levels and TSC2 does not have the catalytic arginine finger found in Ras-GAP. (asm.org)
  • We showed that CRACR2A (Ca 2+ release-activated Ca 2+ channel regulator 2A) encodes a lymphocyte-specific large Rab GTPase that contains multiple functional domains, including EF-hand motifs, a proline-rich domain (PRD), and a Rab GTPase domain with an unconventional prenylation site. (sciencemag.org)
  • growth factor stimulation
  • It is likely that there are many more interdomain collaborations with the new discovered SH2-like domain-for example, the observed recruitment of GIV to the PM after growth factor stimulation is likely to be cooperatively mediated by its phosphatidylinositol 4-phosphate (PI4P) binding (Enomoto et al. (nih.gov)
  • EGFR
  • Frequent molecular alterations of NSCLC include mutations/deletions of FHIT , p53 , p16, LKB1 and K-Ras genes, as well as overexpression/mutation of EGFR or HER2 and increased telomerase activity. (pubmedcentralcanada.ca)
  • agonist
  • Accordingly, the mechanisms mediating agonist control of Ras-GDP/GTP levels have been the focus of intense research. (biomedcentral.com)
  • stimuli
  • At the cellular level, meaningful responses to external stimuli depend on propagation and quenching of a signal to maintain vigilance for subsequent stimulation or changes that serve to shape and modulate the response. (frontiersin.org)
  • catalytic
  • We demonstrated a novel catalytic mechanism of the TSC2 GAP and Rheb that TSC2 uses a catalytic "asparagine thumb" instead of the arginine finger found in Ras-GAP. (asm.org)
  • upstream
  • Computational analyses revealed enrichment of many sequence motifs within the upstream and downstream intronic regions near the alternatively spliced regions corresponding to binding sites of splicing regulators. (health-media.net)
  • Cdc42
  • Moreover, we show that the potentiating effect of ionomycin on Ras-GRF-mediated MAPK stimulation is also regulated by Cdc42. (hud.ac.uk)
  • GAPs
  • The GAP function of the amino-terminal extension of ARD1 required two arginines, an intact zinc finger motif, and a group of residues which resembles a sequence present in Rho/Rac GAPs. (embl-heidelberg.de)
  • membrane
  • Cell functions are accomplished by highly coordinated activity of several membrane‐enclosed organelles that exchange chemicals and information. (embopress.org)
  • MCSs are few tens to hundreds nm in size and typically use a small fraction of the membrane of each organelle, namely 2-3% in the resting state that may increase to 5-10% after cell stimulation, except in yeast in which the ER‐PM contacts are formed with as much as 40% of the PM [ 9 ]. (embopress.org)
  • tumor
  • We provide evidence that neurofibromin, the product of the tumor suppressor of neurofibromatosis type 1 (NF1), is the RasGAP species mediating the deactivation of Ras. (biomedcentral.com)
  • inhibition
  • In contrast, the mechanism accounting for signal termination and prompt restoration of basal Ras-GTP levels is unclear, but has been inferred to involve feedback inhibition of Sos. (biomedcentral.com)
  • Our findings show that, in addition to feedback inhibition of Sos, feedback stimulation of the RasGAP neurofibromin enforces termination of the Ras signal in the context of growth-factor signaling. (biomedcentral.com)
  • activate
  • Identification of Epac as an intermediary linking the GLP-1-R to the stimulation of insulin secretion has led to an appreciation that the blood glucose-lowering effect of GLP-1 might be reproduced using pharmacological agents that activate cAMPGEFs in a direct and selective manner. (diabetesjournals.org)
  • secretion
  • Alterations in pulsatile stimulation of the gonadotropes result in differential synthesis and secretion of the gonadotropins LH and FSH and changes in the expression of their respective hormone subunit genes. (frontiersin.org)
  • cell
  • Of particular note are new reports demonstrating that Epac couples β-cell cAMP production to the stimulation of fast Ca 2+ -dependent exocytosis. (diabetesjournals.org)
  • Extensive work has elucidated that the duration of the signal elicited by Ras is decisive for cell fate decision taking. (biomedcentral.com)
  • substrate
  • Although the FLVRES motif and Arg βB5 play a dominant role in SH2-mediated phosphopeptide binding, they do not fully account for the specificity of SH2 domain substrate interaction. (asm.org)
  • We found that Arg15, a residue equivalent to Gly12 in Ras, is important for Rheb to function as a substrate for TSC2 GAP. (asm.org)
  • biochemical
  • Both genetic and biochemical studies have demonstrated that Rheb (Ras homolog enriched in brain) is a direct target of TSC2. (asm.org)