Stimulation of peripheral blood lymphocytes. Medical search. Frequent questions

The Company's state-of-the-art and patented Todos Biochemical Infrared Analyses (TBIA) is a proprietary cancer-screening technology using peripheral blood analysis that examines cancer's influence on the immune system, looking for biochemical changes in blood mononuclear cells and plasma. (yahoo.com)
Tumor-infiltrating lymphocytes (TIL) and autologous peripheral blood mononuclear cells (PBMC) were isolated from patients undergoing surgery for primary tumors. (aacrjournals.org)
We now validated whether [ 18 F]FB-IL2 can be used to quantify activated human peripheral blood mononuclear cells (hPBMC) in rats by pharmacokinetic modelling. (springer.com)
In our previous studies, we demonstrated the ability of [ 18 F]FB-IL2 to selectively detect activated human peripheral blood mononuclear cells (hPBMC) in rodent models of inflammation by noninvasive PET imaging [ 22 ]. (springer.com)
These results provide preliminary evidence that MBSR(BC) increases TA in peripheral blood mononuclear cells from BC patients and have implications for understanding how MBSR(BC) may extend cell longevity at the cellular level. (virginia.edu)
These cells inhibited T cell proliferation in a standard in vitro mixed lymphocyte assay and, moreover, attenuated the development of vasculopathy mediated by autologous peripheral blood mononuclear cells in a functionally relevant humanized mouse transplant model. (lu.se)
Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. (thermofisher.com)

Pokeweed mitogen is also used in research studies to stimulate different lymphocytes and observe change. (wikipedia.org)

MART-1 is an Ag expressed on melanomas and melanocytes, and is recognized by the majority of HLA-A2-restricted tumor-specific tumor-infiltrating lymphocytes (TIL) from melanoma patients. (elsevierpure.com)
These processes are impeded by PD-1, a coinhibitory molecule expressed on T cells that is elevated in tumor-infiltrating lymphocytes (TIL). (nih.gov)
Adoptive cell therapy using tumor-infiltrating lymphocytes (TILs) has shown activity in melanoma, but has not been previously evaluated in metastatic non-small cell lung cancer. (nature.com)

PWM can contribute to B-cell assay or other lymphocytes for testing. (wikipedia.org)
Evaluation of lymphocyte function was approached with a lymphocyte blastogenesis (stimulation) assay as it can be adapted to all species. (vin.com)
The choice of assay should be based on: 1) the type of pathogen causing the infection and the body system of the patient that is most frequently infected, 2) breed, age, and history of the patient, 3) evaluation of the complete blood count (CBC). (dvm360.com)
In colorectal biopsies and peripheral blood lymphocytes of patients with IBD, polyps and CRC, we evaluated 1) genomic instability using the γH2AX assay as marker of DSB and micronuclei in mononuclear lymphocytes kept under cytodieresis inhibition, and 2) cellular stress through expression and cellular localization of glutathione-S-transferase omega 1 (GSTO1). (oncotarget.com)
The measurement of iATP levels in peripheral blood CD4+ T-lymphocytes following thir nonspecific stimulation with phytohemagglutinin (PHA), through the commercial assay Immuknow®, was approved by US Food and Drug Administration for detecting cell-mediated immunity in immunosuppressed patients. (ucm.es)
4 ]. A relationship between activated or lymphocytes and enzyme-linked immuno- memory-effector lymphocyte populations sorbent assay (ELISA) for CD117. (who.int)

It can test for immunodeficiency by inducing cell proliferation and test for abnormal counts of lymphocytes. (wikipedia.org)
The Lymphocyte Proliferation Test (LymPro Test™) is a diagnostic blood test that determines the ability of peripheral blood lymphocytes (PBLs) and monocytes to withstand an exogenous mitogenic stimulation that induces them to enter the cell cycle. (yahoo.com)
It has also been implicated in proliferation and differentiation of preactivated B lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. (lu.se)
Ba inhibits the proliferation of preactivated B lymphocytes. (lu.se)
I. Effect on lymphocyte proliferation and interleukin 2 receptor expression. (typeset.io)
Selenium (Se) is an essential nutritional factor that was shown by us to alter the expression of the high affinity interleukin 2 receptor (Il2-R) and its subunits, cell proliferation, and clonal expansion of cytotoxic T-lymphocytes in mice. (typeset.io)
These molecular defects interfere with lymphocyte development and function, blocking the differentiation and proliferation of T cells and, in some types, of B cells and NK cells. (medscape.com)
In X-linked SCID, loss of IL-2 receptor (IL-2R) function leads to the loss of a lymphocyte proliferation signal. (medscape.com)
To analyse cell-mediated responses to GAD, we studied the peripheral blood lymphocyte proliferation and cytokine responses to recombinant human GAD-65 in 5 patients with SMS, 6 with CAPA, 9 with DM1, 8 with APS and 15 control subjects. (uab.cat)

LymPro is unique in the use of peripheral blood lymphocytes as a surrogate for neuronal cell function, suggesting a common relationship between PBLs and neurons in the brain. (yahoo.com)
Human peripheral blood lymphocytes (PBLs) were from mice infected with a SAg-producing S. pyogenes purified from blood of a healthy donor by using strain (29). (cdc.gov)
Importantly for future clinical trials, stimulation of peripheral blood lymphocytes (PBLs) from a melanoma patient by AdCMVMAGE- 1-transduced autologous cells resulted in the generation of specific CTLs against the MAGE-1 antigen. (illinois.edu)

3 Gy) in the number of leucocytes (i.e., lymphocytes, monocytes, basophils, eosinophils and neutrophils) by the 30th day post autopsy interval in comparison to the control. (tfljournal.org)

MART-1 27-35 -specific CTL could be effectively generated from a total of 11 of 12 PBL and from 3 of 3 TIL derived from HLA-A2 + melanoma patients, as well as from 2 of 4 PBL from HLA-A2 + healthy donors by in vitro stimulation with autologous PBMC pulsed with the synthetic MART-1 27-35 peptide. (elsevierpure.com)
These disorders are characterized by the activation of the immune system and slow recruitment of immune cells (peripheral blood mononuclear cell, PBMC) in the lesion. (springer.com)
Tetramer staining of fresh, unstimulated PBMC from two of the DNA-vaccinated animals indicated that as much as 0.4% of all CD3+/CD8alpha+ T lymphocytes were specific for the SIVgag CTL epitope. (ox.ac.uk)
Administration of MVA expressing the SIVgag CTL epitope further boosted these responses, such that 0.8-20.0% of CD3+/CD8alpha+ T lymphocytes in fresh, unstimulated PBMC were now Ag specific. (ox.ac.uk)
Moreover, direct ex vivo SIV-specific cytotoxic activity could be detected in PBMC from five of the six DNA/MVA-vaccinated animals, indicating that this epitope-based DNA prime/MVA boost regimen represents a potent method for inducing high levels of functionally active, Ag-specific CD8+ T lymphocytes in non-human primates. (ox.ac.uk)
Expanded T cells are screened in stimulations with pathogen proteins in the presence of autologous PBMC as antigen-presenting cells (APC). (antigendiscovery.com)

The CD27 antigen is a lymphocyte-specific member of the tumor necrosis factor receptor (TNFR) super family, which also includes nerve growth factor receptor, CD30, CD40, CD95 (Fas), CD120a, CD120b, CD134 (OX 40), and CD137. (bdbiosciences.com)
the kinetics of the response showed that indomethacin must be added to lymphocyte cultures within hours after mitogen or antigen addition for enhancement of [3h]thymidine uptake to occur. (liverpool.ac.uk)
Although it is possible to expand naturally occurring T(regs), an attractive alternative possibility, particularly suited to solid organ and bone marrow transplantation, is the stimulation of total T cell populations with defined allogeneic antigen-presenting cells (APCs) under conditions that lead to the generation or expansion of donor-reactive, adaptive T(regs). (lu.se)

Although anti-PD-1 antibodies enhance IFNγ secretion after stimulation of the T-cell receptor (TCR), the mechanistic link between PD-1 and its effects on T-cell help (Tc1/Th1 skewing) remains unclear. (nih.gov)

High levels of interleukin-2 receptors (IL-2R) can be found mainly on the surface of activated T lymphocytes (both CD4+ and CD8+, mainly Th1 lymphocytes) [ 1 - 3 ] after endogenous stimulation. (springer.com)
There was a clear correlation between supplementation with Se and enhanced 3H-thymidine incorporation into nuclear DNA, preceded by enhanced expression of high affinity Il2-R. Supplementation with Se can apparently modulate T-lymphocyte mediated immune responses in humans that depend on signals generated by the interaction of interleukin 2 with Il2-R. (typeset.io)
Calcium ionophore, ionomycin, and phorbol myristate acetate (PMA) were used to activate rabbit peripheral blood B cells to study the role of increased intracellular calcium ion concentration ( (Ca$\sp2+\rbrack\sb{\rm i}$), protein kinase C (PKC) activation, and autocrine interleukin (IL-2) in inducing cell cycle entry and maintaining activation to DNA synthesis. (tmc.edu)

Figure 1: CD3/CD46 stimulation induces IL-10 production in human peripheral blood CD4 + T lymphocytes. (nature.com)

The autoimmune nature of diabetes and inflammatory cytokine IL-10 (as markers the major contribution of lymphocyte T- of inflammatory changes) and the soluble cells are well established. (who.int)

Human peripheral blood CD3 + T lymphocytes were isolated using Human T-cell Isolation kit (Miltenyi, DEU). (biomedcentral.com)
The issue of the tropicity of the SARS-CoV-2 virus to human nervous tissue and its possible ways of affecting the peripheral nervous system is discussed. (bvsalud.org)
Premature chromosome condensation in human resting peripheral blood lymphocytes without mitogen stimulation for chromosome aberration analysis using specific whole chromosome DNA hybridization probes. (uams.edu)
This study examined the effect of dietary (200 μg/d for 8 wk) supplementation with selenium (as sodium selenite) on the ability of human peripheral blood lymphocytes to respond to stimulation with alloantigen, develop into cytotoxic lymphocytes, and to destroy tumor cells, and on the activity of natural killer cells. (typeset.io)
The finding that many human melanomas express distinct antigens that can be recognised by specific cytolytic T lymphocytes (CTL) implies that immunotherapeutic strategies against this cancer might prove effective. (illinois.edu)

In prospectively collected cancer tissues, we found that TIL exhibited dampened Tc1/Th1 skewing and activation compared with peripheral blood lymphocytes (PBL). (nih.gov)
CD3/CD28 T-cell activation Dynabeads (Gibco, USA) was used for T cell stimulation in the presence of IL-2 (PeproTech, USA). (biomedcentral.com)
A number of the immunologic abnormalities seen in SARD, including changes in peripheral B (switched memory) and T (iNKT, T regulatory, activated memory T follicular helper) subsets and B cell activation, were also seen in asymptomatic ANA + subjects and those with UCTD. (biomedcentral.com)
In this study, we examined peripheral blood T and B cell populations and their activation in asymptomatic ANA + individuals together with patients with undifferentiated connective tissue disease (UCTD) and early SARD to determine whether the cellular immune characteristics found in SARD are distinct from those seen in asymptomatic ANA + individuals, most of whom will not progress to SARD. (biomedcentral.com)
Next, the effect of complete and incomplete signalling on B lymphocyte activation leading to cell cycle entry, IL-2 production, functional IL-2 receptor (IL-2R) expression, and DNA synthesis was examined. (tmc.edu)
Incomplete activation with ionomycin or PMA alone altered the responsiveness of B cells to further stimulation only in the case of ionomycin, and the unresponsiveness of these cells was apparently due to a lack of functional IL-2R expression on these cells, even though IL-2 production was maintained. (tmc.edu)

The MM group showed a decrease of perforin level in CD8 + lymphocytes after stimulation compared with patients with PL. These results indicate that MM patients have characteristics of impairment in stimulation-induced cytotoxicity of peripheral blood CD8 + lymphocytes and that PL and MM patients have a common character of functional alteration in those lymphocytes, namely, an increase in memory cells, possibly related to exposure to asbestos. (elsevierpure.com)
The data indicated that the supplementation regimen resulted in 118% increase in cytotoxic lymphocyte-mediated tumor cytotoxicity and 82.3% increase in natural killer cell activity as compared to baseline values. (typeset.io)

The amplified number of proliferated cells makes it easier to test different lymphocyte functions, biological mechanisms, and response to different stimuli. (wikipedia.org)
When looking at deficiency of CD26 in knockout mice, after stimulation with PWM the role of CD26 in development of CD4+ T cells was observed. (wikipedia.org)
To test whether extracts also affected normal cells, similar experiments were also performed using isolated peripheral blood lymphocytes from healthy volunteers, with and without stimulation by the mitogen phytohemagglutinin (PHA). (nih.gov)
The CD27 antibody, clone L128, is derived from hybridization of mouse Sp2/0 cells with spleen cells from BALB/c mice immunized with activated peripheral blood lymphocytes. (bdbiosciences.com)
Enzyme-linked immunospot assays confirmed this high frequency of Ag-specific cells, and intracellular IFN-gamma staining demonstrated that the majority of these cells produced IFN-gamma after peptide stimulation. (ox.ac.uk)
Fig. 5: Change in phenotype and genotype of peripheral T cells after TIL infusion. (nature.com)
TRV-β sequencing of tumor and peripheral T cells as the project 'TIL trial for NSCLC' are available in the immuneACCESS free public database at https://clients.adaptivebiotech.com/immuneaccess . (nature.com)
We examined the properties of peripheral blood CD8 + lymphocytes from asbestos-exposed patients with pleural plaque (PL) and MM. The percentage of CD3 + CD8 + cells in PBMCs did not differ among the three groups, although the total numbers of PBMCs of the PL and MM groups were lower than those of the healthy volunteers (HV). (elsevierpure.com)
The percentage of IFN-γ + and CD107a + cells in PMA/ionomycin-stimulated CD8 + lymphocytes did not differ among the three groups. (elsevierpure.com)
Percentages of perforin + cells and CD45RA - cells in fresh CD8 + lymphocytes of PL and MM groups were higher than those of HV. (elsevierpure.com)
Percentages of granzyme B + and perforin + cells in PMA/ionomycin- stimulated CD8 + lymphocytes were higher in PL group compared with HV. (elsevierpure.com)
Percentages of perforin+ cells and CD45RA- cells in fresh CD8+ lymphocytes of PL and MM groups were higher than those of HV. (elsevierpure.com)
Biomarkers included common lymphocyte subsets in the peripheral blood as well as the ability of T cells to become activated and secrete cytokines in response to stimulation with mitogens. (virginia.edu)
The numbers and percentages of lymphocyte subsets, activated T cells, and Th1 and Th2 cells in peripheral blood samples were determined by immunostaining and flow cytometry. (virginia.edu)
Here we demonstrate that stimulation of mouse CD4(+) T cells by immature allogeneic dendritic cells combined with pharmacological inhibition of phosphodiesterase 3 (PDE) resulted in a functional enrichment of Foxp3(+) T cells. (lu.se)
While the autoreactive cells displayed significantly reduced Tregs numbers, the alloreactive transplacentally acquired maternal lymphocytes had high functional Tregs. (hindawi.com)
In some cases, T cells may present, as a result of residual autologous cells or transplacentally acquired maternal lymphocytes [ 1 ]. (hindawi.com)
The origin of the reactive cells in such patients are either thymic release of T-cells that expand at the periphery or transplacentally transfers of maternal T lymphocytes. (hindawi.com)
These cells expand in the periphery, causing tissue infiltration and damage due to breakdown of both central (e.g., autoimmune regulator, AIRE protein dysfunction) and peripheral (FOXP3 + deficiency) tolerance mechanisms [ 3 ]. (hindawi.com)
For example, Omenn syndrome, a typical case of impaired T-cell differentiation with abnormal self-reactive cells, is invariably characterized by autoimmune features such as generalized scaly exudative erythroderma, enlarged lymphoid tissues, and peripheral expansion of oligoclonal T-cells, in addition to increased susceptibility for severe infections [ 3 , 4 ]. (hindawi.com)
A distinctive feature of SCID patients, which sometimes can clinically resemble Omenn, [ 6 ] is the presence of alloreactive cells originated from transplacentally maternal T lymphocytes. (hindawi.com)
The hypothesis that IL-2, acts in late G$\sb1$ and is required for DNA synthesis in B cells was supported by comparing IL-2 production and DNA synthesis in peripheral blood cells and purified B cells, kinetic analysis of these events in B cells, effects of anti-IL-2 antibody and PKC inhibitors, and by the response of G$\sb1$ B cells. (tmc.edu)
In contrast, only T cells from CAPA patients showed a significantly high production of interferon-γ after GAD stimulation, compared to all other patients and controls. (uab.cat)
Lymphomas are cancers of a specific type of white blood cells known as lymphocytes. (msdmanuals.com)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
HN - 2008 BX - Granulosa Cells, Cumulus MH - Coronary Sinus UI - D054326 MN - A07.231.908.194.500 MS - A short vein that collects about two thirds of the venous blood from the MYOCARDIUM and drains into the RIGHT ATRIUM. (bvsalud.org)

The CD27- subset of peripheral blood memory CD4+ lymphocytes contains functionally differentiated T lymphocytes that develop by persistent antigenic stimulation in vivo. (bdbiosciences.com)

Peripheral blood lymphocytes (PBL) from 35 donors were tested for IL-2 production after in vitro stimulation by different rabies and rabies-related viruses. (researchgate.net)

1. CABG increased peripheral blood lymphocyte apoptosis. (czytelniamedyczna.pl)

There were no MF-related changes in NK activity or the number of circulating neutrophils, eosinophils, basophils, or T-lymphocytes (CD4, CD8, CD4:CD8 ratio). (cdc.gov)

Another study where PWM was used as a stimulus was in the proteome analysis of lymphocytes of different immune phenotypes in cows. (wikipedia.org)
The effects of a 60-Hz magnetic field (MF) exposure on white blood cell ornithine decarboxylase (ODC) activity, natural killer (NK) cell activity, lymphocyte phenotypes, and differential cell counts were studied among 60 electric utility workers. (cdc.gov)

Peripheral blood cellular immunological changes were assessed by flow cytometry and transcript levels of BAFF , interferon (IFN)-induced and plasma cell-expressed genes were quantified by NanoString. (biomedcentral.com)
CD44 percentage values in peripheral T-lymphocytes were significantly higher in patients than controls, as detected by flow cytometry. (who.int)

The suggested mechanism for this phenomenon is the possible inability of the thymus to delete these abnormal clones due to compromise of both central and peripheral tolerance mechanisms [ 5 ]. (hindawi.com)

T lymphocytes and cytokines are believed to cause the characteristic inflammatory changes appreciated on histopathologic examination of lesions. (medscape.com)

Interrelationships and determinants of aging biomarkers in cord blood. (who.int)

This study shows that dietary supplementation of Se-replete humans with 200 micrograms/d of sodium selenite for 8 wk, or in vitro supplementation with 1 x 10(-7) M Se (as sodium Se) result in a significant augmentation of the ability of peripheral blood lymphocytes to respond to stimulation with 1 microgram/mL of phytohemagglutinin or alloantigen and to express high affinity Il2-R on their surface. (typeset.io)

Because the quantity and function of lymphocyte subsets change during stress, we hypothesized that these subsets would serve as stress markers and therefore predict which breast cancer patients would benefit most from mindfulness-based stress reduction (MBSR)-facilitated stress relief. (virginia.edu)
The aim of the study was to analyse the mitochondrial transmembrane potentials of lymphocytes in adult patients undergoing coronary artery bypass grafting (CABG). (czytelniamedyczna.pl)
Le pourcentage de CD44 dans les lymphocytes T périphériques était significativement plus élevé chez les patients que chez les témoins, comme détecté par la cytométrie en flux. (who.int)
En outre, il y avait une aug- mentation significative de la forme soluble du c-kit dans le sérum des patients atteints de pemphigus vulgaire actif par rapport aux témoins. (who.int)
Les IFN et les IL-10 étaient signi cativement élevés chez ceux qui présentaient une néphropathie diabétique (ND) et une maladie rénale en phase terminale (MRPT) par rapport aux témoins et aux patients diabétiques sans ND. (who.int)

Van Parijs, A. & Abbas, A. K. Homeostasis and self-tolerance in the immune system: turning lymphocytes off. (nature.com)

Efforts were made to evaluate immunologic health via leukocyte phenotyping and lymphocyte function. (vin.com)

Beside other apoptotic signals in lymphocyte injury, the decrease of mitochondrial transmembrane potential is regarded as the earliest and non-reversible. (czytelniamedyczna.pl)

Neoantigen-reactive T cell clonotypes increased and persisted in peripheral blood after treatment. (nature.com)
Graves disease may be associated with normocytic anemia, low-normal to slightly depressed total white blood cell (WBC) count with relative lymphocytosis and monocytosis, and low-normal to slightly depressed platelet count. (medscape.com)
Beyond this basic grouping, SCID may be categorized according to phenotypic lymphocyte profiles that include both B-cell status (B + or B - ) and NK-cell status (NK + or NK - ) in addition to T-cell status (T - , because there is always a T-cell deficiency in SCID). (medscape.com)

There is no specific or diagnostic blood test for psoriasis. (medscape.com)

M (CTPYDINQM)-specific CD8+ T lymphocyte responses in six of six Mamu-A*01+ rhesus macaques. (ox.ac.uk)
enhancement of lymphocyte blastogenic and delayed hypersensitivity skin responses by indomethacin. (liverpool.ac.uk)

A complete blood count (CBC) with differential should be obtained at baseline and with the development of fever or symptoms of infection. (medscape.com)

IL-2 was produced by PBL from more than 85% of donors after stimulation with inactivated and purified rabies viruses (IPRV) prepared from either Pittman Moore (PM) or Pasteur Virus (PV) strains. (researchgate.net)

We suggest that a high-tolerance capacity of the alloreactive transplacentally acquired maternal lymphocytes represents a toleration advantage, yet still associated with severe immunodeficiency. (hindawi.com)

Exposure to PCBs has been assessed by measuring PCBs in blood, breast milk, and adipose tissue. (cdc.gov)

Anatomy50

Organisms11

Diseases21

Chemicals and Drugs90

Analytical, Diagnostic and Therapeutic Techniques and Equipment45

Phenomena and Processes47

Disciplines and Occupations1

Information Science3

Health Care3

Mononuclear cells7

Pokeweed Mitogen1

Tumor-infiltratin3

Assay6

Proliferation9

PBLs3

Monocytes1

PBMC6

Antigen3

Secretion after stimulation1

Interleukin3

Induces1

Cytokine1

Human5

Activation6

Cytotoxicity2

Cells27

Functionally1

Vitro stimulation1

Apoptosis1

Basophils1

Phenotypes2

Flow cytometry2

Central and peripheral1

Cytokines1

Biomarkers1

Phytohemagglutinin1

Patients5

Immune system1

Immunologic1

Signals1

Cell3

Diagnostic blood test1

Responses2

Differential1

Donors1

Tolerance1

Breast milk1