This metabolic step is the first in sterol biosynthesis leading to the formation of lanosterol or cycloartenol. (gerli.com)
Cell membrane4
S)-2,3-Oxidosqualene ((S)-2,3-epoxysqualene) is an intermediate in the synthesis of the cell membrane sterol precursors lanosterol and cycloartenol, as well as saponins. (wikipedia.org)
Mechanism of Action of Azoles Azoles exert their action by inhibiting the C14α demethylation of lanosterol in fungi, which interferes with the synthesis of ergosterol in the fungal cell membrane. (davidgessner.com)
The antifungal azoles target fungal ergosterol biosynthesis by inhibiting the fungal CYP450-dependent enzyme lanosterol 14-α-demethylase, which blocks the conversion of lanosterol to ergosterol and leads to accumulation of aberrant sterol compounds and depletion of ergosterol in the fungal cell membrane. (davidgessner.com)
Azole antifungals work by inhibiting the cytochrome P450 dependent enzyme lanosterol 14-alpha-demethylase, which converts lanosterol to ergosterol, the main sterol in the fungal cell membrane. (davidgessner.com)
Squalene3
ERG1 and ERG7 encode squalene epoxidase and lanosterol synthase, respectively, which are two important, unique and essential enzymes in the ergosterol synthesis pathway. (davidgessner.com)
Nearly 200 different triterpene skeletons are known from natural sources and represent structurally cyclization products of Squalene which is the immediate biological precursor of all triterpenoids. (gerli.com)
The stereoisomer 2,3-(R)-oxidosqualene is an inhibitor of lanosterol synthase. (wikipedia.org)
Hydroxymethylglutaryl-CoA synthase 1 and 2 (HMG-CoA synthase 1 and 2) The first enzyme in the lanosterol pathway catalyses the synthesis of the metabolite (S)-3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) from condensation and acyl group transfer of substrates acetyl CoA and acetoacetyl CoA. (davidgessner.com)
The first committed enzyme in triterpene saponin biosynthesis from A. mongolicus , cycloartenol synthase (AmCAS), which belongs to the oxidosqualene cyclase family, was cloned by us to study the astragalosides biosynthesis. (biomedcentral.com)
Biosynthesis4
As a member of this family, lanosterol 14α-demethylase is responsible for an essential step in the biosynthesis of sterols. (davidgessner.com)
Deficiencies in sterol biosynthesis cause pleiotropic defects that limit cellular proliferation and adaptation to stress. (davidgessner.com)
Which antifungal class targets the biosynthesis of lanosterol in the fungal membrane? (davidgessner.com)
Six CsOSCs were characterized for the biosynthesis of diverse triterpene skeletons, including α-amyrin, ß-amyrin, δ-amyrin, dammarenediol-II, ψ-taraxasterol, taraxasterol, and cycloartenol by the heterologous expression system. (bvsalud.org)
Cholesterol2
Lanosterol, a precursor of cholesterol, is ingested with human diet and occurs in the body in concentrations exceeding solubility. (davidgessner.com)
The steroid hormones progestagens, estrogens, androgens, and glucocorticoids as well as their precursor cholesterol are required for successful establishment and maintenance of pregnancy and proper development of the fetus. (chateaunut.com)
Enzyme1
Imidazoles inhibit the enzyme lanosterol-14-α-demethylase, a cytochrome P-450-dependent enzyme that converts lanosterol to ergosterol. (davidgessner.com)
Synthesis1
By blocking fungal cytochrome P450-dependent enzymes, azoles disrupt the synthesis of ergosterol, which is the principal sterol in fungal cell membranes. (davidgessner.com)
Natural2
Lanosterol is a natural product found in Eleutherococcus sessiliflorus, Euphorbia mellifera, and other organisms with data available. (davidgessner.com)
They form a large group of natural products which includes steroids and consequently sterols, they derived from C30 precursors. (gerli.com)