Stem differentiation into tissue. Medical search. Frequent questions

OBJECTIVE: To evaluate the different behavior of two different human adult adipocytes derived stem cells (hASCs) during proliferation and osteogenic differentiation. (uniba.it)
After osteogenic differentiation, the cell clusters were incubated and analyzed with Real Time-Polymerase Chain Reaction (qRT-PCR) and fluorescence microscopy. (uniba.it)
The ALP, AR stain confirmed the osteogenic differentiation capacity of MSCs towards osteoblast-like-cells. (uniba.it)
This study was therefore aimed at establishing the dose-response effect of EPO stimulation on rat BMSCs and investigating the effects of mechanical stretch combined with EPO on the proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
The proliferation and osteogenic differentiation of rat BMSCs were examined and compared using EPO with different concentrations. (hindawi.com)
The osteogenic differentiation of BMSCs was detected by alkaline phosphatase (ALP) staining, and the mRNA expression levels of ALP, OCN, COL, and Runx2 were detected by qPCR. (hindawi.com)
Mechanical stretch combined with 20 IU/ml EPO promoted proliferation and osteogenic differentiation of BMSCs. (hindawi.com)
EPO was able to promote the proliferation and osteogenic differentiation of BMSCs, and these effects were enhanced when combined with mechanical stretch. (hindawi.com)
Notably, it promotes the osteogenic differentiation of bone mesenchymal stem cells (BMSCs) [ 15 - 17 ]. (hindawi.com)
This study was therefore aimed at addressing three key issues: First, is there a dose-dependent effect of EPO on the proliferation and osteogenic differentiation of BMSCs? (hindawi.com)
The cells were phenotypically categorized according to the expression of CD29, CD90, CD34 and CD45, and submitted to adipogenic and chondrogenic differentiation for 21 days and osteogenic differentiation for 7, 14 and 21 days. (scielo.br)
On days 7, 14 and 21 of osteogenic differentiation, the cultures were submitted to evaluations of MTT conversion in formazan, of alkaline phosphatase activity (AP), of collagen and mineralized matrix synthesis, evaluation of the number of cells per field and there was quantification of the gene transcripts for osterix, bone sialoprotein (BSP), osteonectin (ON) and osteocalcin (OC). (scielo.br)
AT-MSC submitted to osteogenic differentiation showed higher MTT conversion in formazan than BM-MSC, under the same conditions on days 7 and 21. (scielo.br)
At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
Osteogenic differentiation of electrostimulated human mesenchymal stem" by ANIL SERA ÇAKMAK, SONER ÇAKMAK et al. (tubitak.gov.tr)
The aim of this study was to investigate the osteogenic differentiation potential of human mesenchymal stem cells (hMSCs) cultivated on silk-fibroin films in response to different parameters, i.e. (tubitak.gov.tr)
The experimental groups can be defined as high voltage in osteogenic differentiation medium, low voltage in osteogenic differentiation medium, and low voltage in growth medium in this study. (tubitak.gov.tr)
Compared to the unstimulated controls (silk films without platinum wires), low voltage (10 mV) did not influence proliferation, while it enhanced osteogenic differentiation according to early and late osteogenic markers in osteogenic differentiation medium. (tubitak.gov.tr)

Injectable Peptide Decorated Functional Nanofibrous Hollow Microspheres to Direct Stem Cell Differentiation and Tissue Regeneration. (stembook.org)
The hydrogels can spontaneously adjust their microstructure to effectively support the massive proliferation, rapid assembly and directional differentiation of stem cells in the three-dimensional hydrogels, thereby promoting the repair and regeneration of damaged tissues and organs. (edu.hk)
CONCLUSIONS: These in vitro findings of both hAS-SCs and hAV-SCs suggested an important role of these stem cells for future clinical use in bone regeneration. (uniba.it)
The project will focus on investigating through which mechanisms senescent cells induce pluripotency (I), how such a persistent lock of stemness can impair tissue regeneration (II) and how this may affect tumor therapy resistance (III). (uu.nl)
Tissue regeneration relies on faithful differentiation of stem cells. (uu.nl)
In healthy cells, we hypothesize that SASP factors prevent differentiation of tissue stem cells and thereby impair tissue rejuvenation (de Keizer Trend Mol Med 2017) Together, this calls for investigation on how senescent and stem cells interact and how mitigation of their negative interplay may improve tissue regeneration during aging and affect migration of therapy-resistant cancer. (uu.nl)
Electric field is known as an important regulator to guide the development and regeneration of many tissues. (tubitak.gov.tr)
The combination of inductive scaffold materials with stem cells might optimize the approaches for bone regeneration. (bvsalud.org)
The development of new biomaterials for tissue engineering provides a scientific basis for the creation of scaffolds that could provide appropriate regeneration and tissue repair 14 . (bvsalud.org)
The high regeneration potential has aroused a great interest in the scientific community 3 , due to its many clinical applications in cell therapy or tissue engineering. (bvsalud.org)
The tissues that used to be discarded may now serve as a basis for scientific research and clinical use in tissue regeneration and treatment of many diseases 23 . (bvsalud.org)
It is used in various applications for its tissue regeneration and immunomodulation activities. (bvsalud.org)
In case of tissue damage, SCs also drive regeneration of the lost tissue. (medscape.com)
By contrast, transit amplifying cells and differentiated cells are incapable of self-renewal and tissue regeneration. (medscape.com)
At the heart of this trade-off is stem cell capacity - while stem cell maintenance is crucial for longevity (e.g., for tissue regeneration to repair damage), stem cell differentiation is required for fertility (i.e., to produce gametes). (lu.se)

Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
However, the results indicate that bone marrow -derived MSCs are superior to those from subcutaneous adipose tissue in terms of differentiation into pancreatic islet -like clusters. (bvsalud.org)
All MSCs clusters were cultured with an osteogenic and adipogenic differentiation medium. (uniba.it)
Homogeneous pools of Mesenchymal Stem Cells (MSCs) were confirmed by Flow-Cytometry Analysis (FACS) and Spectrophotometric Assay. (uniba.it)
Mesenchymal stem cells (MSCs) are multipotent cells with regenerative and immunomodulatory properties. (frontiersin.org)
Mesenchymal stem cells (MSCs) are multi-potent cells that can differentiate into osteoblasts, adipocytes, chondrocytes and myocytes. (harvard.edu)
MSCs isolated from MSCKO mice showed reduced differentiation towards osteoblasts and chondrocytes in vitro, but no difference in proliferation or apoptosis. (harvard.edu)
The efficacy and safety profile of mesenchymal stem cells (MSCs) augmentation in chondral procedures are controversial. (springer.com)
We found that myocilin is expressed in bone marrow-derived mesenchymal stem cells (MSCs) and affects their differentiation into osteoblasts. (nih.gov)
Mesenchymal Stem Cells (MSCs) can be isolated from several body tissues, including dental tissues. (bvsalud.org)
MSCs have great therapeutic potential in tissue engineering, they can be expanded in vitro, and combined with scaffolds they can be inserted into wounds to promote healing and tissue replacement. (bvsalud.org)
The ability to restore cells and tissue function without the need of immunosuppressive drugs and without the concern for tissue compatibility makes Mesenchymal Stem Cells (MSCs, usual acronym) a strong promise for the future. (bvsalud.org)
MSCs have a clinical attractiveness because they are easy to expand, they have the ability to differentiate into various types of tissues 5 , and can be directed to become osteocytes, chondrocytes or adipocytes. (bvsalud.org)

Furthermore, mechanical stretch may induce the differentiation of BMSCs into mature osteoblasts and enhance the deposition of the bone matrix [ 18 - 20 ]. (hindawi.com)
My research continues to focus on stem cells and osteoblasts and in particular 3D cell culture models and microenvironments. (nottingham.ac.uk)
Stem cells differentiate into bone-forming osteoblasts, and mechanical stimulation is involved in this p. (biomedcentral.com)

Using biomaterials to study stem cell mechanotransduction, growth and differentiation" (PDF). (wikipedia.org)
Continuous growth and differentiation of organoids depend on both Wnt and Notch paracrine signalling. (nature.com)
Support the growth and differentiation of human pluripotent stem cells by using Vitronectin XF™, a defined and xeno-free cell culture matrix. (stemcell.com)
For stem cells to be used in tissue engineering a scaffold is essential to provide the necessary support for the transport of nutrients, oxygen and the elimination of metabolic waste 30 , promoting a conducive environment for cell growth and differentiation. (bvsalud.org)

Olfactomedin 1 (Olfm1) and the closely related genes Olfactomedin 2 (Olfm2) and Olfactomedin 3 (Olfm3) show overlapping expression patterns demonstrating preferential expression in the developing and adult neuronal tissues. (nih.gov)
In contrast to recent findings in neuronal differentiation, we found that mitochondrial content decreases continuously during mesoderm differentiation, despite increased mitochondrial activity and higher levels of ATP-linked respiration. (uib.no)
Neuroblastoma tumor initiating cells are immature with a neural crest-like phenotype and downregulation of HIF-2alpha in these cells results in neuronal sympathetic differentiation and the cells become phenotypically similar to the bulk of neuroblastoma cells found in clinical specimens. (lu.se)
Through understanding functional recovery in terms of neuronal subtype and connectivity, the work presented in this thesis aims to bring the prospect of CRT closer to the clinic, I also describe the generation of a very promising alternative cell source that could rival fetal tissue. (lu.se)

We explore the method on three circuits for haematopoiesis and embryonic stem cell development for commitment and reprogramming scenarios and illustrate how the method can be used to determine sequential steps for onsets of external factors, essential for efficient reprogramming. (lu.se)

Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
Differentiation of mesenchymal stem cells derived from human bone marrow and subcutaneous adipose tissue into pancreatic islet-like clusters in vitro. (bvsalud.org)
Professor Bian explained that the supramolecular hydrogels provide an excellent three-dimensional cell culture experiment tool for basic biomedical research such as stem cells and tumour in vitro models, and can be used as an effective delivery vehicle for therapeutic cells to serve many transformational types including regenerative medicine. (edu.hk)
We show that single epithelial stem cells in vitro can give rise to differentiated organoids containing ciliated and secretory cells. (nature.com)
The presence of 'stem cell-like' cells has previously been postulated based on sphere-forming capacity and differentiation in vitro 1 and evidence for the presence of label-retaining cells in the distal fallopian tube 2 . (nature.com)
Here we demonstrate the existence of adult stem cells in the human fallopian tube epithelium, which gives rise to a monolayer of differentiated epithelial cells in a complex 3D organoid in vitro . (nature.com)
Soft tissue engineering for both clinical use and as novel 3D in vitro models for improved laboratory studies. (sheffield.ac.uk)
This protocol describes a detailed procedure for resuspending and culturing human stem cell derived neurons that were previously differentiated from neural progenitors in vitro for multiple weeks. (jove.com)
Our approach is to develop in vivo and in vitro models of musculoskeletal injury and clinically-translatable outcome measures to assess the efficacy of regenerative and tissue engineering solutions. (rochester.edu)
Discuss how we can use stem cells combined with biomaterials for in vitro and in vivo applications. (manchester.ac.uk)
It is possible to grow retinal tissue in vitro . (labmanager.com)
In this regard, tissue engineers have sought to leverage native mechanotransduction pathways to enhance in vitro stem cell. (biomedcentral.com)
Tissue engineering has developed many paradigms and techniques on how to best integrate cells and extracellular matrix to create in vitro structures that replicate native tissue. (biomedcentral.com)
Second, altering the MSC state by inducing differentiation into multiple lineages did not affect the exosomal properties or endocytosis but triggered the expression of lineage-specific genes and proteins in vitro and in vivo respectively. (frontiersin.org)
An in vitro and in vivo comparison among three different human hepatic stem cell populations. (ca.gov)
Dynamic Compressive Loading Improves Cartilage Repair in an in vitro Model of Microfracture: Comparison of Two Mechanical Loading Regimens on Simulated Microfracture Based on Fibrin Gel Scaffolds Encapsulating Connective Tissue Progenitor Cells. (chop.edu)
In vitro repair of meniscus radial tear using hydrogels seeded with adipose stem cells and TGF-β3. (chop.edu)

It is one of a number of analysis and characterisation facilities within the Department, and is predominantly used by members of the Biomaterials and Tissue Engineering theme . (sheffield.ac.uk)
Develop an understanding of the principles of tissue engineering, the biomaterials commonly used and discuss some specific examples of engineered tissues. (manchester.ac.uk)
Advances in the fields of stem cell biology, biomaterials, and tissue engineering over the last decades have brought the possibility of constructing tissue substitutes with a broad range of applications in reg. (biomedcentral.com)
Jewell's research combines immunology and biomaterials to understand the interactions between synthetic materials and immune tissues, and to design more selective therapeutic vaccines for cancer and autoimmunity. (jhu.edu)

Be able to design a scaffold to direct differentiation. (manchester.ac.uk)

Anatomical region-specific enhancement of 3-dimensional chondrogenic differentiation of human mesenchymal stem cells by soluble meniscus extracellular matrix. (chop.edu)

These genes increase the proliferation and differentiation of cells associated with the hair follicle matrix. (medlineplus.gov)

Journal of Tissue Engineering and Regenerative Medicine. (wikipedia.org)
Embryonic stem cells hold considerable promise in tissue engineering and regenerative medicine as a source of tissue-specific cells. (ca.gov)

This protocol facilitates the use of human pluripotent stem cell-derived neurons for high content screening applications. (jove.com)
Human pluripotent stem cell-derived neurons are increasingly relevant in the areas of basic research, drug development, and degenerative medicine. (jove.com)

As a carrier of stem cells, the gels with ultra-dynamic mechanical properties encapsulated with the cultivated stem cells can be injected into the defect site to support the spreading and directed differentiation of the cells in them. (edu.hk)

We aim to develop a cell culture platform to automatically differentiate human induced pluripotent stem cells (hiPSCs) for lab-on-a chip applications. (dim-elicit.fr)
The results reveal that mesenchymal stem cells (MSC) derived from bone marrow and subcutaneous adipose tissue can differentiate into pancreatic islet -like clusters, as evidenced by their islet-like morphology, positive dithizone staining and expression of genes such as Nestin , PDX1, Isl 1, Ngn 3, Pax 4 and Insulin . (bvsalud.org)
Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
The goal of this protocol is to test the ability of progenitor cells derived from human perivascular adipose tissue to differentiate into multiple cell lineages. (jove.com)
Differentiation was compared to mesenchymal stem cells derived from human bone marrow, which is known to differentiate into adipocyte, osteocyte, and chondrocyte lineages. (jove.com)

In conclusion, micropatterned co-cultures of mESCs and hepatic cells showed a significant promise in driving stem cell differentiation towards hepatic phenotype. (ca.gov)
The HIF-2alpha-Driven Pseudo-Hypoxic Phenotype in Tumor Aggressiveness, Differentiation, and Vascularization. (lu.se)

The aim of this paper is to provide a review about current and future materials for scaffolds to carry stem cells in tissue engineering in Dentistry, especially for bone tissue repair. (bvsalud.org)

They can be used as a carrier that mimics the human extracellular matrix, encapsulating human stem cells or the patient's autologous cells, and be implanted into the patient's tissue defect site so as to promote tissue repair and achieve an ideal therapeutic effect. (edu.hk)
Efficacy of thermoresponsive, photocrosslinkable hydrogels derived from decellularized tendon and cartilage extracellular matrix for cartilage tissue engineering. (chop.edu)

According to rough calculations, the amount of culture medium needed to produce enough erythrocytes for a normal transfusion pack (containing one trillion units) is 1,000 -- 2,000 liters when using direct differentiation from undifferentiated iPS cells, whereas 50-100 liters is sufficient with the newly developed method. (sciencedaily.com)

Given the considerable interest in using stem cells for modeling and treating disease, it is essential to understand what regulates self-renewal and differentiation. (uib.no)
Tumors, like the tissues from which they originate, contain a unique SC population that is capable of self-renewal and of sustaining tumor growth indefinitely. (medscape.com)
Furthermore, pathways controlling the self-renewal capacity of normal tissue SCs are frequently deregulated in human cancer. (medscape.com)
Finally, there is a discussion about the mechanisms behind the self-renewal and differentiation of stem cells and the role of stem cells in renewal of the body's tissues. (lu.se)

However, their pancreatic lineage transdifferentiation competence was not compared to that for bone marrow -derived stem cells . (bvsalud.org)
The pancreatic lineage differentiation was further corroborated by positive results in the glucose challenge assay. (bvsalud.org)
Many existing tissue-engineering approaches focus on delivery of selected proteins (growth factors, transcription factors etc.) or nucleic acids to host or implanted stem cells to achieve lineage specific differentiation. (frontiersin.org)
However, realizing this promise requires novel methods for guiding lineage-specific differentiation of stem cells. (ca.gov)
Thus, our work highlights similarities in mitochondrial remodeling during the transition from pluripotent to multipotent state in ectodermal and mesodermal lineages, while at the same time demonstrating cell-lineage-specific adaptations upon further differentiation. (uib.no)
A fraction of ADIPOSE TISSUE prepared to enrich in STEM CELLS with the capacity for multi-lineage differentiation. (bvsalud.org)

Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
Here, we address the question of mitochondrial adaptation by examining the differentiation of human pluripotent stem cells to cardiac progenitors and further to differentiated mesodermal derivatives, including functional cardiomyocytes. (uib.no)

Microarray analysis reveals that inhibition of Notch signalling causes downregulation of stem cell-associated genes in parallel with decreased proliferation and increased numbers of ciliated cells and that organoids also respond to oestradiol and progesterone treatment in a physiological manner. (nature.com)
Furthermore, Notch is responsible for the regulation of known stem cell factor genes. (nature.com)
Our data reveal a significant overlap between Notch-dependent genes in the fallopian epithelium and the defined 'stem cell signature' of the mouse intestine, suggesting the existence of a conserved pathway that regulates tissue renewal and directly controls cell fate specification and differentiation in the organoid by inhibiting cilliogenesis. (nature.com)
Deep sequencing followed by abundancy ranking pinpointed six top hit genes potentially associated with either improved or impaired endodermal differentiation, which were selected for functional validation in CRISPR-Cas9 mediated knockout (KO) lines. (mdpi.com)
We conclude that SIRT1 deacetylates β-catenin to promote its accumulation in the nucleus leading to transcription of genes for MSC differentiation. (harvard.edu)
We focus our attention on the genes that are expressed in RGCs and eye angle tissues. (nih.gov)

inbook{ecf3c49f-5038-4d94-a89d-062e1056050f, abstract = {{Cellular adaptation to diminished tissue oxygen tensions, hypoxia, is largely governed by the hypoxia inducible transcription factors, HIF-1 and HIF-2. (lu.se)

This project is part of RESCUE, a multidisciplinary, intersectoral and interdisciplinary PhD training programme in Regenerative Medicine and Stem Cells organised by UMC Utrecht (coordinator) and Utrecht University. (uu.nl)
The course aims to prepare students for work in a field that includes regenerative medicine and stem cells. (lu.se)

Indeed, the final outcomes suggested a better performance of cells coming from subcutaneous adipose tissue vs. those from visceral fat tissue. (uniba.it)

Erythrocytes are produced in the body by progressive evolution of hematopoietic stem cells into hematopoietic progenitor cells and then erythroblasts. (sciencedaily.com)

Until now, several progenitor cells derived from dental tissues have been isolated and characterized ( table I ). (bvsalud.org)

PATIENTS AND METHODS: Human adult adipocytes stem cells (hAT-SCs) from visceral (hAV-SCs) and subcutaneous (hAS-SCs) sites were obtained after surgery procedures of seven patients. (uniba.it)

The retina is a tissue of the eye that consists of several layers of neurons forming a chain. (labmanager.com)
This involves placing stem cell clusters into a special medium that induces the spontaneous formation of undeveloped neurons, followed by their differentiation into retinal cells. (labmanager.com)
That process produces actual retinal neurons organized as complex tissue, without any external stimulation of development pathways during specialization. (labmanager.com)
A potentially pre-clinical aspect of this thesis is detailed in paper №4 where I describe a robust protocol for the generation of functional mesDA neurons from human embryonic stem cells that are functional in a rat model of PD. (lu.se)

My PhD focussed on investigating the distributions and possible functions of nitric oxide/nitric oxide synthases in the physiology and pathology of a variety of tissues and organs and I was fortunate enough to publish some of the first papers on the involvement of these molecules in human diseases, notably in the cardiovascular system. (nottingham.ac.uk)
Human mesenchymal stem cells (HMSCs) are multipotent somatic stem cells that can be isolated from a variety of tissues such as the bone marrow, adipose tissue, and dental pulp. (frontiersin.org)

On examination of the oral cavity, the vascular malformations of the mucosa and the adjacent soft tissues are usually readily apparent. (medscape.com)

Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)

The major advantage of these stem cells over their counterparts is their amazing proliferative and differentiation potency . (bvsalud.org)
Normal proliferative tissues contain multipotent stem cells (SCs) that drive tissue turnover. (medscape.com)

Detailed characterization of cell type transitions is essential for cell biology in general and particularly for the development of stem cell-based therapies in regenerative medicine. (nature.com)
I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
On completion of the course, the students shall be able to · reflect on societal opportunities and consequences arising from developmental biology research and knowledge, and discuss these with individuals in the same education cycle · reflect on current scientific challenges and ethical issues in developmental and stem cell biology research and how this influences society in general. (lu.se)

The unit will demonstrate various types of stem cells: adult, embryonic, induced pluripotent and their specialised culture methods. (manchester.ac.uk)
As a result of being capable of differentiating into a variety of cell types, it can be presumed that stem cell therapy has an advantage when compared to other tissue repair methods. (bvsalud.org)

Although stem cells are present in various adult tissues and body fluids , bone marrow has been the most popular source of stem cells for treatment of a wide range of diseases . (bvsalud.org)
In adult tissues, this pathway plays a role in the maintenance and renewal of stem cells, which are cells that help repair tissue damage and can give rise to other types of cells. (medlineplus.gov)
Stem cells are classified into two main: embryonic stem cells, which are found in the embryos and adult stem cells, found in adult tissues. (bvsalud.org)

Here we report on the establishment of long-term, stable 3D organoid cultures from human fallopian tubes, indicative of the presence of adult stem cells. (nature.com)
We also developed a tool for automatic retina identification and organoid classification, which we showed in action, optimizing cell specialization conditions and monitoring tissue quality. (labmanager.com)
Robustly generate three-dimensional, patterned brain organoid cultures from human pluripotent stem cells without matrix embedding by using STEMdiff™ Midbrain Organoid Differentiation Kit. (stemcell.com)

An article collection in Stem Cell Research & Therapy . (biomedcentral.com)
Cell therapy is a therapy where cellular material is injected systemically or directly into the injured tissue, to promote local repair or to restore systemic health. (bvsalud.org)

Cellular adaptation to diminished tissue oxygen tensions, hypoxia, is largely governed by the hypoxia inducible transcription factors, HIF-1 and HIF-2. (lu.se)

These rare tumors arise from connective tissue, which provides strength and flexibility to structures such as bones, ligaments, and muscles. (medlineplus.gov)
Knockdown of HIF-2alpha in neuroblastoma and glioma tumor stem/initiating cells leads to reduced levels of VEGF and poorly vascularized, highly necrotic tumors. (lu.se)
The cancer stem cell theory holds that tumors also contain stem-like cells that drive tumor growth and metastasis formation. (medscape.com)

The concept of tissue engineering is to develop biological materials to repair, replace and improve the damaged organs and their functions in the human body. (edu.hk)
At the Stem Cell Differentiation and Cytogenetics Group, we are interested in the effects of ionizing radiation on organs such as the brain, the heart, and the lung trying to elucidate the risks for patients treated with ionizing radiation as well as for astronauts, who are inevitably exposed to ionizing radiation on their missions into space. (gsi.de)
Discuss the importance of mimicking structures of tissues and organs. (manchester.ac.uk)
Tissue engineering is the science that combines the principles of biology and engineering techniques in order to obtain biological substitutes for regenerating, replacing, modifying, repairing or restoring the function of organs and tissues. (bvsalud.org)

Recent results for stem cells from adipose tissue have put it in a position to compete for being the leading therapeutic source. (bvsalud.org)
Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
The potential therapeutic use of stem cells has been broadly researched in recent years. (bvsalud.org)
The stem cells from exfoliated deciduous teeth (SHEDs) have a great potential for therapeutic use because of its differentiation capability and its easy access, since the collection is performed at the physiological exfoliation stage of the deciduous teeth. (bvsalud.org)

In such organoids, we can also mimic brain tumor initiation and growth and examine the interaction between normal tissue and tumor tissue upon treatment with ionizing radiation alone or in combination with other treatment modalities such as chemotherapies. (gsi.de)
In recent years, high tumor cell levels of HIF-2 and the oxygen sensitive subunit HIF-2alpha have been associated with unfavorable disease and shown to be highly expressed in tumor stem/initiating cells originating from neuroblastoma and glioma, respectively. (lu.se)

However during aging the regenerative capacity of tissues is impaired and the inhibitory mechanism on tissue stem cells are largely unknown When healthy cells are irreparably damaged, they can enter a state called senescence (White et al. (uu.nl)
However, the role of stem cell capacity in life-history trade-off has not been investigated. (lu.se)
In this project, we will manipulate stem cell capacity in Drosophila melanogaster flies by controlling their oxygen-sensing conditions (e.g., by keeping them under different oxygen concentrations or by using transgenic flies), and we will investigate whether and how fertility, longevity, and (potentially) tissue repair differ between flies with high or low stem cell capacity . (lu.se)

Use Vitronectin XF™ with mTeSR™1 , mTeSR™ Plus , TeSR™-E8™ , or TeSR™-AOF medium to provide a defined culture system for the maintenance of human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells under feeder-free conditions. (stemcell.com)

By using this platform, time and efforts will be reduced and both cardiac and alveolar models will be studied for tissue repairing assays. (dim-elicit.fr)

Proteomics is emerging as a powerful tool to identify the signaling complexes and pathways that control multi- and pluri-potency and SC differentiation. (medscape.com)

His research is focused on mesenchymal stem cell interactions with nanotopography, with particular focus on the use of metabolomics, to study mechanotransduction. (wikipedia.org)

My research focuses primarily on tissue engineering of load bearing connective tissues, including ligament and tendon, cartilage, and bone. (rochester.edu)

Anatomy71

Organisms8

Diseases8

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes57

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science3

Osteogenic differentiation18

Regeneration15

MSCs13

Osteoblasts3

Growth and differentiation4

Neuronal4

Embryonic stem1

Vitro17

Biomaterials4

Scaffold1

Chondrogenic differentiation1

Proliferation and differentiation1

Engineering and Regenerative Medicine2

Pluripotent stem cell-de2

Cultivated stem cells1

Differentiate5

Phenotype2

Scaffolds1

Extracellular matrix2

Direct differentiation1

Self-renewal4

Lineage6

Cardiomyocytes2

Genes6

Abstract1

Regenerative Medicine a2

Coming from subcutaneous adipose1

Hematopoietic Stem1

Progenitor cells derived1

Subcutaneous1

Neurons4

Variety of tissues2

Soft Tissues1

Pancreatic1

Proliferative2

Cell biology3

Methods2

Adult tissues3

Organoid3

Therapy2

Transcription1

Tumors3

Organs4

Therapeutic4

Tumor2

Capacity3

Human induced1

Cardiac1

Pathways1

Mechanotransduction1

Connective1