Stem cells mice clone cells. Medical search. Frequent questions

There are important problems in the therapeutic use of cloning which increase with the more advanced differentiation of somatic cells. (yildiz.edu.tr)
In humans, CD38 is expressed by the majority of hematopoietic cells at levels which vary according to the differentiation and activation status of the cells. (stemcell.com)
Is the Subject Area "Cell differentiation" applicable to this article? (plos.org)
Here we document the effects of specified PEMF parameters over mesenchymal stem cells (MSC) chondrogenic differentiation. (nature.com)
Mesenchymal stem cells (MSCs) support chondrogenic differentiation and are an attractive cell source for cartilage tissue engineering. (nature.com)
The expression of TRA-1-60 antigen is stage-specific and can be used to characterize embryonic cells and monitor their differentiation. (bdbiosciences.com)
As human EC and ES cells undergo differentiation, expression of TRA-1-60 antigen is lost. (bdbiosciences.com)
Ezh2 orchestrates gene expression for the stepwise differentiation of tissue-specific stem cells. (genscript.com)
Our scientists have developed a wide array of stem cell-focused reagents and resources for many applications including flow cytometry , western blotting , ELISAs , and recombinant proteins for cell differentiation. (biolegend.com)
RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. (biolegend.com)
These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)
However, the use of bFGF for human ES cell culture, particularly at the high levels used by some for 'feeder-free' culture, must be considered carefully, as bFGF may drive ES cells towards differentiation. (ddw-online.com)
The need to control differentiation of embryonic stem cells in vitro presents another set of challenges. (ddw-online.com)
Accumulating evidence suggests that KDM4A is not only involved in body homeostasis (such as cell proliferation, migration and differentiation, and tissue development) but also associated with multiple human diseases, especially cancers. (bvsalud.org)
Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)

France prohibits reproductive cloning and embryo creation for research purposes, but enacted laws (with a sunset provision expiring in 2009) to allow scientists to conduct stem cell research on imported a large amount of embryos from in vitro fertilization treatments. (wikipedia.org)
A high-resolution anatomical atlas of the transcriptome in the mouse embryo. (genscript.com)
The resulting embryo was then used to create embryonic stem cells, capable of generating every cell type in the body, and the nuclei of these cells were injected into other eggs to produce clones. (newscientist.com)
After many divisions in culture, this single cell forms a blastocyst (an early stage embryo with about 100 cells) with almost identical DNA to the original donor who provided the adult cell - a genetic clone. (eurostemcell.org)
Embryonic stem cells can then be harvested from the cloned embryo and used to create new cells and organs for the original organism. (kottke.org)
That's why Father Pacholczyk, director of education at the National Catholic Bioethics Center in Philadelphia, said that the efforts to help people understand the immorality of embryo reserch, including human cloning, must focus on humanizing the issue and appreciating our own embryonic origins, not just on the desired results of embryonic or other types of stem-cell research. (archstl.org)
When Yamanaka's team implanted the cells into mouse embryos, those embryos developed as expected - with the DNA of the original stem cell, not of the embryo. (denialism.com)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)
For instance, he wonders-just an intellectual puzzle, he assures me, that he would never want to do-What would happen if scientists injected human stem cells into a monkey embryo? (discovermagazine.com)
Nearly all the cells in the stalled embryo had turned into brain cells, simply because a single protein had been stopped. (discovermagazine.com)

This Study presents the first use in somatic cell nuclear transfer (SCNT) of tooth Pulp cells containing progenitor cells that resemble the nonaging embryonic connective tissue in adults. (yildiz.edu.tr)
Using tooth Pulp Cells ill SCNT increased the first cleavage rates compared with adult somatic Cells, leading to a higher rate of DNA reprogramming and increased production of an identical embryonic stem cells (ESCs) line. (yildiz.edu.tr)
These Outcomes are promising for regenerative medicine ill the therapy of diabetes mellitus, and clarification of the importance of selecting appropriate adult somatic cells in deriving identical ESC lines is also a significant step. (yildiz.edu.tr)
While embryonic stem cells are taken from embryoblasts, induced pluripotent stem cells are undifferentiated from somatic adult cells. (wikipedia.org)
Stem cell treatments are a type of cell therapy that introduce new cells into adult bodies for possible treatment of cancer, somatic cell nuclear transfer, diabetes, and other medical conditions. (wikipedia.org)
Cloning, or somatic cell nuclear transfer (SCNT), is the technique used to produce Dolly the sheep, the first animal to be produced as a genetic copy of another adult. (eurostemcell.org)
It became a hot topic in 1996 when Dolly the sheep was cloned via a process called somatic cell nuclear transfer. (archstl.org)
Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)
if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)

Therapeutic Use of Cloning: Osmangazi Tu. (yildiz.edu.tr)
This proposed South Korean program, led by Woo Suk Hwang of Seoul National University, will store human ES cells, and will focus on their therapeutic uses. (progress.org.uk)
Belgium bans reproductive cloning but allows therapeutic cloning of embryos. (wikipedia.org)
Sweden forbids reproductive cloning, but allows therapeutic cloning and authorized a stem cell bank. (wikipedia.org)
In therapeutic cloning, the blastocyst is not transferred to a womb. (eurostemcell.org)
Another long-term hope for therapeutic cloning is that it could be used to generate cells that are genetically identical to a patient. (eurostemcell.org)
To date, no human embryonic stem cell lines have been derived using therapeutic cloning, so both these possibilities remain very much in the future. (eurostemcell.org)
NEW YORK, September 21, 2003 - New research from Memorial Sloan Kettering Cancer Center (MSKCC), Cornell University, and The University of Connecticut describes a novel way of producing therapeutic nerve cells that can cure mice with Parkinson's-like disease. (mskcc.org)
5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)
The use of mouse feeder layers and animal serum are particularly problematic in the culturing of stem cells for possible therapeutic applications. (ddw-online.com)

Stem cells have been used to repair tissue damaged by disease. (wikipedia.org)
The Wakayama team used a modified version of a cloning technique in which the nucleus of a mouse cell - in this case a cell from dead tissue that has been frozen and then thawed - is injected into a mouse egg that has had its nucleus removed. (newscientist.com)
In a surprise finding, the Wakayama team discovered that it was easiest to create clones from brain tissue, even though clones have never been created from living brain cells. (newscientist.com)
Wakayama speculates that freezing and thawing the tissue somehow makes it easier to "reprogram" the brain cell nucleus. (newscientist.com)
Brain tissue is also high in sugars, which can protect cells when they freeze. (newscientist.com)
But this is the first time animals have been cloned from lumps of tissue frozen without the use of chemicals that might protect the cells from damage. (newscientist.com)
The accomplishment, known as hematopoietic transdifferentiation, may prove useful for tapping the potential for tissue repair using human adult stem cells. (scienceblog.com)
A small percentage of AND-34 −/− mice show distinctive small white eye lesions resulting from the migration of ruptured cortical lens tissue into the anterior chamber. (molvis.org)
CD106 antigen is present on activated endothelial cells, tissue macrophages, dendritic cells and bone marrow fibroblasts. (beckman.com)
The team first made mouse PGCs, and then added cells taken from the testicular tissue of newborn mice, as well as other biological molecules. (scientificamerican.com)
8 Jun, 2007 04:13 pm Stem cells provide the starting material for the development and repair of every organ and tissue in the body and they are present in all stages of life. (scitizen.com)
We are a global provider of human and animal biospecimens: including frozen & FFPE tissue, DNA, RNA, total proteins, blood products and primary cells. (amsbio.com)
And the researchers have already accomplished the first step, creating embryonic stem-like cells from the tissue of an endangered adult snow leopard ( Panthera uncia ). (scientificamerican.com)
The activation of these cells leads to the release of chemical mediators such as histamine and arachidonic acid metabolites, which increase vascular permeability and tissue swelling 9 . (bvsalud.org)

All recurrent mutations identified in mononuclear cells could be tracked back to the phenotypically defined hematopoietic stem cell (HSC) compartment in all investigated patients and were also present in downstream myeloid and erythroid progenitor cells. (nih.gov)
Progenitor cells are similar to embryonic stem cells in their capacity to differentiate into various cell types. (ddw-online.com)
However, progenitor cells can only differentiate into a limited number of cell types. (ddw-online.com)
Progenitor cells can be far easier to handle in culture than ES cells. (ddw-online.com)
For example, neural progenitor cells derived from a human ES cell line are easily propagated and require less handling than human ES cells. (ddw-online.com)
1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)

Stem cell laws are the law rules, and policy governance concerning the sources, research, and uses in treatment of stem cells in humans. (wikipedia.org)
Embryonic stem cells were isolated in mice in 1981, and in humans in 1998. (wikipedia.org)
Potentially easier would be cloning cryogenically frozen humans, though the consensus among cloning experts is that it would be unethical and dangerous to clone a human. (newscientist.com)
In most countries, it is illegal to attempt reproductive cloning in humans. (eurostemcell.org)
American scientific companies are cross-breeding humans and animals and coming up with mice with fully functioning human brains," O'Donnell said. (livescience.com)
2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)
Our results showed that inflamed and non-inflamed dental pulps neither from humans nor from rats presented mast cells. (bvsalud.org)
Since mast cells are active during inflammatory responses it becomes crucial to find out if these cells are actually present and play a role in dental pulp under normal and inflammatory conditions either in humans or in rats. (bvsalud.org)
Therefore, the purpose of the present work was, under the same experimental conditions, to identify mast cells in healthy and inflamed dental pulp from rats and humans using toluidine blue histochemistry and immunohistochemistry techniques. (bvsalud.org)
The demonstration of what could happen was great, and they used mice because it's a more tractable system, but what we cared about were humans. (medscape.com)

When the cloning process is used in this way, to produce a living duplicate of an existing animal, it is commonly called reproductive cloning. (eurostemcell.org)
The fact that the resulting cell could be injected into an egg and produce a viable animal is a stringent test," says Allan Spradling, a reproductive biologist at the Carnegie Institution for Science in Baltimore, Maryland. (scientificamerican.com)
2. Over the years, the international community has tried without success to build a consensus on an international convention against the reproductive cloning of human beings. (who.int)
3. Creating awareness among ministries of health in the African Region will provide them with critical and relevant information on the reproductive cloning of human beings and its implications to the health status of the general population. (who.int)
7. The WHO Regional Committee for Africa is invited to review this document for information and guidance concerning reproductive cloning of human beings. (who.int)
3. Media reports on nuclear transfer are usually about one form, reproductive nuclear transfer, also known as reproductive cloning of human beings . (who.int)
Some prohibit only cloning for reproductive purposes and allow the creation of cloned human embryos for research, whereas others prohibit the creation of cloned embryos for any purpose. (who.int)
Elaboration of an international convention against reproductive cloning of human beings has been under consideration in the United Nations since December 2001 when the subject was included in the agenda of the fifty- sixth session as a supplementary agenda item at the request of France and Germany. (who.int)

To produce Dolly, the cloned blastocyst was transferred into the womb of a recipient ewe, where it developed and when born quickly became the world's most famous lamb. (eurostemcell.org)
Instead, embryonic stem cells are isolated from the cloned blastocyst. (eurostemcell.org)
For blastocyst, five samples (including ICM and TE) for control (ICSI) and semi-cloned blastocysts (including WT and DKO). (nih.gov)

He stayed on to earn his Ph.D. in molecular biology at Cambridge, training under the legendary geneticist John Gurdon, whose breakthroughs in the 1950s and 1960s were key to the experiments performed by Ian Wilmut, a Gurdon student who cloned Dolly the sheep in 1997. (discovermagazine.com)
Although many species produce clonal offspring in this fashion, Dolly, the lamb born in 1996 at a research institute in Scotland, was the first asexually produced mammalian clone. (who.int)

WHA50.37 of 1997 argues that human cloning is ethically unacceptable and contrary to human integrity and morality. (who.int)
General Assembly the following year,3 and the World Medical Association's Resolution on Cloning, endorsed in 1997, have confronted the issue but lack binding legal force. (who.int)

European scientists aim to create a library of mouse embryonic stem cells that can be used to research human diseases. (progress.org.uk)
Woo Suk Hwang - head of the team that announced the creation of 11 patient-specific embryonic stem (ES) cell-lines recently - is planning to open an international stem cell bank in South Korea. (progress.org.uk)
A team of South Korean researchers have managed to extract embryonic stem (ES) cells from frozen human embryos. (progress.org.uk)
The information presented here covers the legal implications of embryonic stem cells (ES), rather than induced pluripotent stem cells (iPSCs). (wikipedia.org)
In contrast to multipotent stem cells, embryonic stem cells are pluripotent and are thought to be able to give rise to all cells of the body. (wikipedia.org)
In January 2007, researchers at Wake Forest University reported that "stem cells drawn from amniotic fluid donated by pregnant women hold much of the same promise as embryonic stem cells. (wikipedia.org)
Whereas Germany, Austria, Italy, Finland, Portugal and the Netherlands prohibit or severely restrict the use of embryonic stem cells, Greece, Sweden, Spain and the United Kingdom have created the legal basis to support this research. (wikipedia.org)
According to modern stem cell researchers, Spain is one of the leaders in stem cell research and currently has one of the most progressive legislations worldwide with respect to human embryonic stem cell (hESC) research. (wikipedia.org)
H9 human embryonic stem (ES) cells were harvested with Accutase™ and stained with either PE Mouse Anti-Human TRA-1-60 (solid line) or PE mouse IgM (G155-228) isotype control (Cat. (bdbiosciences.com)
The antigen is found on teratocarcinoma (embryonal carcinoma or EC), embryonic inner cell mass (but not morula or trophoblast), and embryonic stem (ES) cells. (bdbiosciences.com)
BayGenomics: a resource of insertional mutations in mouse embryonic stem cells. (genscript.com)
Rb homeostasis is also essential for self-renewal and survival of human embryonic stem cells 10 . (biolegend.com)
They coaxed mouse embryonic stem cells to become cells that resembled primordial germ cells (PGCs)-an important stage in the development of both eggs and sperm. (scientificamerican.com)
generated haploid male gametes from mouse embryonic stem cells that can produce viable and fertile offspring, demonstrating functional reproduction of meiosis in vitro. (scientificamerican.com)
Scientists have found a way to possibly avoid using embryonic stem. (scitizen.com)
18 Sep, 2007 12:13 pm Rice University researchers have engineered musculoskeletal cartilages with human embryonic stem cells, with the hope of eventually using the neotissue. (scitizen.com)
These cells have been described as embryonic stem cells (ESCs) and. (scitizen.com)
6 Jun, 2007 07:00 pm Researchers have reprogrammed skin cells into embryonic stem cells. (scitizen.com)
Cloning + embryonic stem cells is a powerful combination. (kottke.org)
It seems the research O'Donnell was referring to was a 2005 study in which fetal mice were injected with human embryonic stem cells . (livescience.com)
To assess the role of an individual HDAC isoenzyme in physiology and tumor development, HDAC2-mutant mice were generated from a gene trap embryonic stem cell clone. (aacrjournals.org)
Father Tad Pacholczyk is convinced that embryonic stem cells will someday cure diseases. (archstl.org)
A decade later, cloning came to the forefront in Missouri with the narrow passage of Amendment 2, a ballot initiative in 2006 that constitutionally protects embryonic stem-cell research and human cloning. (archstl.org)
Our lab first derived mouse androgenetic haploid embryonic stem cells (AG-haESCs) and demonstrated that AG-haESCs can be used as an "artificial spermatids" to generate gene-edited semi-cloned (SC) mice through intracytoplasmic injection (ICAHCI) into mature oocyte, even though the birth efficiency is very low. (nih.gov)
and from 2002 on pluripotency in embryonic stem cells in the lab of Ali H. Brivanlou. (eurostemcell.org)
He has been leader of a research group at the Max-Delbrück-Center for Molecular Medicine in Berlin until 2011 focusing his research on signal transduction mechanisms in human and murine embryonic stem cells. (eurostemcell.org)
Stem cells, and in particular human embryonic stem (ES) cells, have earned a reputation for being labour intensive and difficult to grow and control in culture. (ddw-online.com)
He and his fellow researchers then used the snow leopard cells to create induced pluripotent stem (iPS) cells, also known as embryonic stem-like cells. (scientificamerican.com)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)

The researchers, based at the Maria Infertility Hospital in Seoul, have obtained seven ES cell lines from 20 embryos left over from infertility treatment. (progress.org.uk)
Because ES cells are cultured from the embryoblast 4-5 days after fertilization, harvesting them is most often done from donated embryos from in vitro fertilization (IVF) clinics. (wikipedia.org)
Italy has a 2004 law that forbids all sperm or egg donations and the freezing of embryos, but allows, in effect, using existing stem cell lines that have been imported. (wikipedia.org)
In 2003, Spain's laws state that embryos left over from IVF and donated by the couple that created them can be used in research, including ES cell research, if they have been frozen for more than five years. (wikipedia.org)
The Catholic Church has always held that stem-cell research and therapies are morally acceptable, as long as they don't involve the creation and destruction of human embryos. (archstl.org)
Is it the idiotic assumption that we are already thinking about injecting these cells willy-nilly into embryos? (denialism.com)

Recent studies shown that CD106 identifies a unique subpopulation of Mesenchymal Stem Cells (MSCs) with powerful immunosuppressive activity. (beckman.com)

A common example of a stem cell is the hematopoietic stem cell (HSC) which are multipotent stem cells that give rise to cells of the blood lineage. (wikipedia.org)

MSCs can be differentiated along different cell lineages of mesodermal origin including osteoblasts, chondrocytes, skeletal myocytes or visceral stromal cells 9 . (nature.com)
It is well-described that leptin receptor (LepR) + perivascular stromal cells provide a major source of bone-forming osteoblasts in adult and aged bone marrow. (nature.com)
These Fgfr3-creER -marked endosteal stromal cells contribute to a stem cell fraction in young stages, which is later replaced by Lepr-cre -marked stromal cells in adult stages. (nature.com)
Further, Fgfr3 + endosteal stromal cells give rise to aggressive osteosarcoma-like lesions upon loss of p53 tumor suppressor through unregulated self-renewal and aberrant osteogenic fates. (nature.com)
These Fgfr3 + stem/stromal cells with OCT identities are abundant in the young bone marrow and depleted in the old bone marrow, denoting their transitional nature. (nature.com)
Overall, our findings indicate that Fgfr3 + endosteal stem/stromal cells with OCT identities dictate active and aggressive osteogenesis, identifying these cells as an important regulator of long-term bone homeostasis. (nature.com)
The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. (origene.com)

We're a partnership of more than 400 stem cell and regenerative medicine labs across Europe, connected via research centres, consortia, networks and hubs. (eurostemcell.org)
Paolo De Coppi is a Consultant Paediatric Surgeon at Great Ormond Street Hospital (GOSH), and Reader and Head of Stem Cells and Regenerative Medicine at the UCL Institute of Child Health in London. (eurostemcell.org)
The remarkable potential of stem cells to generate several hundred differentiated cell types is driving their use for regenerative medicine and for supporting the traditional drug discovery and development process (Figure 1). (ddw-online.com)

Use of feeder layers requires two cell types to be maintained in parallel and introduces mouse proteins into the culture system. (ddw-online.com)
In this fashion, mice or other laboratory animals that exhibit particular traits can be created for specialized studies, or herds of farm animals (such as goats, sheep or cows) can be created that produce pharmaceutically useful proteins in their milk. (who.int)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)

The TRA-1-60 monoclonal antibody reacts with the neuraminidase-resistant form of a pluripotent-stem-cell-specific epitope on a high-molecular-weight transmembrane glycoprotein. (bdbiosciences.com)

Our scientists pursue every aspect of cancer research-from exploring the biology of genes and cells, to developing immune-based treatments, uncovering the causes of metastasis, and more. (mskcc.org)
In 2001, Lorenz Studer, MD , Head of the Stem Cell and Tumor Biology Laboratory at MSKCC, and his colleagues at Rockefeller University published research in which they generated unlimited numbers of genetically matched dopamine nerve cells using cloned stem cells whose genetic material originated from the mouse's own tail. (mskcc.org)
2 Haematopoietic Stem Cell Biology Laboratory, MRC Molecular Haematology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom. (nih.gov)
Glimpse the future of medicine with Kristin Baldwin from the Scripps Research Department of Cell Biology as she discusses cutting-edge stem cell technologies. (scienceblogs.com)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)

These stem cells are genetically matched to the donor organism, holding promise for studying genetic disease. (eurostemcell.org)

It has been reported that the 1A8 antibody detects Ly-6G-expressing granulocytes in peripheral blood, whereas the RB6-8C5 antibody also binds to Ly-6C-expressing lymphocytes, monocytes, and dendritic cells. (stemcell.com)
The CD106 antigen, also known as Vascular Cell Adhesion Molecule-1 (VCAM-1), is an adhesion molecule which binds to integrin α4/β1 (VLA‑4) and integrin α4/β7 on lymphocytes. (beckman.com)

These mice provide evidence that the key functions of HDAC2, although not essential for survival of the organism, play a rate-limiting role for tumor development in vivo . (aacrjournals.org)
A clone is an organism that is a genetic copy of an existing one. (who.int)

Peroxisomes are ubiquitous components of the cytoplasm found in nearly all mammalian cells. (medscape.com)

Assessment of stem cell function in vitro as well as in vivo established that only HSCs and not investigated progenitor populations could propagate the SF3B1 mutated clone. (nih.gov)
Every cell type has its own unique needs when grown in vitro and stem cells are no exception. (ddw-online.com)

Healthy mice have been cloned from cells from dead mice that had been frozen for 16 years, raising the possibility that endangered species could be cloned from old carcasses that have been tossed in freezers, rather than from living cells frozen using elaborate techniques. (newscientist.com)
Globally, there are several cloning programmes that aim to increase the size of rapidly-dwindling populations of endangered species such as African wildcats, and maintain genetic diversity through one-off clonings of individuals that haven't bred . (newscientist.com)
What's more, most conservationists agree that cloning should be considered only as a last resort for species such as the northern white rhino, where all other attempts at conservation have failed, says Paul Bartels, manager of BioBankSA at the National Zoological Gardens of South Africa in Pretoria. (newscientist.com)
Researchers in North Carolina have successfully demonstrated that genetically altered stem cells from one species can be turned into a different sort of cell in another. (scienceblog.com)
But as the Web site Wildlife News points out, techniques such as reproduction with iPS cells could be used when a species is down to its last few members and breeding options are few and far between. (scientificamerican.com)
Most natural cloning occurs in those species that produce their descendants asexually, that is, without combining the male and female genetic material. (who.int)
Recently, research has increased in the area of transplanting embryonic cells across species and growing kidneys and endocrine pancreas cells in situ. (medscape.com)
Heterophilic antibodies, such as rheumatoid factor (RF) and human anti-mouse antibodies (HAMA), can bind to immune globulins of other species and thus interfere with the immunoassay since the tryptase immunoassay employs mouse-derived anti-tryptase. (medscape.com)

The following Vps35l gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)

5' noncoding region] and significantly lower neurovirulence supplementary immunization activity (SIA) to sample collection of fecally shed vaccine virus in transgenic mice) ( 3 - 5 ). (cdc.gov)

In his never-ending quest to attack all science that doesn't affirm his belief that vitamin D and fruit smoothies will cure all disease, he's gone after the new new induced pluripotent stem cell findings . (denialism.com)
Chad and I have both been principal investigators on the induced pluripotent stem cell (IPSC) grant. (medscape.com)
I fell in love with the idea of human pluripotent stem cells and started surveying the United States and even Europe for who was playing in that area. (medscape.com)

TRA-1-60 antigen is released into the serum of patients bearing testicular tumors containing EC cells. (bdbiosciences.com)
Many tumor cell lines and experimental tumors respond to HDAC inhibition. (aacrjournals.org)
But mice cloned in this fashion eventually developed neck tumors. (denialism.com)
It seems that everyone in the mainstream media is so excited about this new stem cell technique that they forgot to notice the fact that it leads to the growth of cancer tumors," said consumer health advocate Mike Adams. (denialism.com)
Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)

This library would make it easier and faster for researchers to obtain mouse stem cells with specific genes already 'knocked-out' so that they will not need to spend the time. (progress.org.uk)
Researchers can 'knock-out', or disrupt, a gene in the mouse to see the effect it has on its health and development. (progress.org.uk)
The stem cells could be studied in the laboratory to help researchers understand what goes wrong in diseases like these. (eurostemcell.org)
Specifically, the researchers converted adult liver stem cells cloned from a male rat into functional adult bone marrow cells in female mice. (scienceblog.com)
Researchers in China say that they have discovered a way to make rudimentary mouse sperm in a dish, and used them to produce offspring. (scientificamerican.com)
Zhou says that the researchers are "very confident" that their protocol can be repeated in other laboratories-and that it would be normal for there to be variations between creating sperm in a dish and the process in live mice. (scientificamerican.com)
Below you can see some examples of the infrastructure for research on genes and cells, available for researchers at Lund University. (lu.se)

Upon transplantation into immune-deficient mice, SF3B1 mutated MDS-RS HSCs differentiated into characteristic ring sideroblasts, the hallmark of MDS-RS. (nih.gov)
Clare's lab recently identified a population of progenitor/stem cells in mice that, on transplantation, is sufficient to generate a fully functional thymus. (eurostemcell.org)
The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)

Inclusion of fetal bovine serum in stem cell media further contributes to an illdefined culture system. (ddw-online.com)

RUNX1-deficient mice fail to generate hematopoietic stem cells. (biolegend.com)
Further we proved that H19-DMR and IG-DMR were the main barrier to generate viable mice through androgenetic and parthenogenetic haESCs. (nih.gov)
More importantly, AG-haESCs mediated SC technology combined with CRISPR-Cas9 is a powerful tool to generate gene-modified mouse models and carry out genetic screening at organismal level. (nih.gov)
Using the SURVEYOR nuclease assay 13 , we assessed the ability of each Cas9-sgRNA complex to generate indels in human embryonic kidney (HEK) 293FT cells through the induction of DNA doublestranded breaks (DSBs) and subsequent nonhomologous end joining (NHEJ) DNA damage repair (Online Methods). (cdc.gov)

For example, stem cells could be generated using the nuclear transfer process described above, with the donor adult cell coming from a patient with diabetes or Alzheimer's. (eurostemcell.org)
The inhibition of CTSB can reduce the brain Aβ peptides and improve memory which was determined from a mouse model with Alzheimer's disease. (biolegend.com)

Transcription factors have an important role in the ability of a cell to self-renew and also differentiate into most cell types, also known as pluripotency 1 . (biolegend.com)

Now, for the first time, scientists have identified a specific molecular pathway within cells that becomes mutated by ultraviolet light exposure, thereby speeding up melanoma development. (scienceblog.com)
But some scientists are not convinced by the report, which is published today in Cell Stem Cell . (scientificamerican.com)
Other scientists raise specific concerns that mainly relate to the timing of the processes that lead to the sperm cells. (scientificamerican.com)
1. Cloning is an umbrella term traditionally used by scientists to describe different processes for duplicating biological material. (who.int)

The underphosphorylated, active form of Rb interacts directly with E2F1 , leading to cell cycle arrest, while the hyperphosphorylated form decouples from E2F1, thus promoting the transcription of genes promoting entry into the S phase. (biolegend.com)
Screening for recurrently mutated genes in the mononuclear cell fraction revealed mutations in SF3B1 in 39 of 40 cases (97.5%), combined with TET2 and DNMT3A in 11 (28%) and 6 (15%) patients, respectively. (nih.gov)
The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
In addition to infrastructures for bioimaging, protein and genes & cells, we also provide other resources e.g., databases, networks and specialized labs. (lu.se)

Alternatively, stem cell cultures can be grown on extracellular matrix extracts and supplemented with conditioned medium from mouse fibroblast cultures. (ddw-online.com)

The 1A8 antibody binds specifically to Ly-6G, whereas another commonly used antibody, clone RB6-8C5, binds to both Ly-6G and Ly-6C. (stemcell.com)
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Mouse Neutrophil Enrichment Kit (Catalog #19762). (stemcell.com)
This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human CD138 Positive Selection Kit II (Catalog #1877) and EasySep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit II (Catalog #17887). (stemcell.com)
antibody (clone AD2.35) on mouse primary astrocytes. (biolegend.com)

For example, Zhou and Zhao report that, on the basis of a genetic analysis, their artificial PGCs were similar to mouse cells at 12.5 days of development. (scientificamerican.com)
However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)

While AND-34 is widely expressed in wild type mice, histologic analysis of multiple organs in AND-34 −/− mice is unremarkable and analyses of lymphocyte development show no overt changes. (molvis.org)
What if your cells could be engineered to grow your own replacement organs? (scienceblogs.com)
The Church also supports research and therapies using adult stem cells, which are cells that come from any person who has been born - including umbilical cord blood, bone marrow, skin and other organs. (archstl.org)
Our facilities provide the opportunity to study molecules, cells, organs and entire organisms. (lu.se)

The cell bank, which cost 2.6 million pounds, is funded by the Medical Research Council (MRC. (progress.org.uk)
The European Union has yet to issue consistent regulations with respect to stem cell research in member states. (wikipedia.org)
Germany has restrictive policies for stem cell research, but a 2008 law authorizes "the use of imported stem cell lines produced before May 1, 2007. (wikipedia.org)
The United Kingdom is one of the leaders in stem cell research, in the opinion of Lord Sainsbury, Science and Innovation Minister for the UK. (wikipedia.org)
A new £10 million stem cell research centre has been announced at the University of Cambridge. (wikipedia.org)
What is cloning, and what does it have to do with stem cell research? (eurostemcell.org)
This form of cloning is unrelated to stem cell research. (eurostemcell.org)
The petition recognizes that many "Canadians suffer from debilitating illnesses and diseases" and that the petitioners "support ethical stem cell research that has already shown encouraging potential to provide cures and therapies for these illnesses and diseases. (lifesitenews.com)
8 Feb, 2008 06:09 pm Six years ago, biomedical engineer Michael King was exploring the strange rolling motion of white blood cells when his research took a radical turn. (scitizen.com)
27 Nov, 2007 10:41 am Tony Maciulis talks with Dr. Jon LaPook about a breakthrough in stem cell research. (scitizen.com)
21 Nov, 2007 10:54 am A huge advancement in stem cell research--and a stake in the heart of human cloning--was announced yesterday. (scitizen.com)
6 Sep, 2007 12:57 pm British authorities decided yesterday to permit research that uses animal eggs to create human stem cells because of the limited supply of human eggs. (scitizen.com)
25 Jun, 2007 04:43 pm On June 7, the House of Representatives voted 247-176 to pass a bill (S 5) that would allow federal funding for research using stem cells derived from. (scitizen.com)
Audience member on the Jewish perspective on stem cell research: "A fetus is a fetus is a fetus until it becomes a lawyer. (kottke.org)
That seems to be the case for Christine O'Donnell, a Tea Party favorite who, during an appearance on The O'Reilly Factor in 2007, made a strange case against cloning and stem-cell research. (livescience.com)
EuroStemCell has key partnerships with European Union-funded stem cell research consortia who provide significant contribution to the project financially and scientifically. (eurostemcell.org)
He co-ordinates the EU Horizon2020 funded stem cell research consortium - INTENS which aims to aims to make a functional reconstructed bowel for people with Short Bowel Syndrome. (eurostemcell.org)
At International Society for Stem Cell Research-the big stem cell meeting-Yamanaka revealed one more factor. (medscape.com)

Depicts a single human stem cell embedded within a porous hydrogel matrix (false colour). (progress.org.uk)

Gr-1 is expressed on monocytes, neutrophils and subsets of macrophages, plasmacytoid dendritic cells, and T cells. (stemcell.com)

General Assembly the adoption of a declaration on human cloning by which Member States were called upon to prohibit all forms of human cloning inasmuch as they are incompatible with human dignity and the protection of human life. (who.int)

These studies have served to highlight the role of AND-34/BCAR3 and p130Cas in cell adhesion and migration signaling pathways. (molvis.org)

Since then, mice have been important in the study of how human diseases progress, because the mouse and the human genomes are so similar. (progress.org.uk)
In sexual reproduction, clones are created when a fertilized egg splits to produce identical (monozygous) twins with identical genomes. (who.int)

The TRA-1-60 antigen is a sialylated epitope on the same keratan sulfate core molecule, podocalyxin, as 4 other distinct antigens on tumor-derived cell lines, TRA-1-81, GCTM2, K4, and K21. (bdbiosciences.com)
Expression of TRA-1-60 antigen has also been observed on a rhesus monkey ES cell line (Thomson et al, 1995). (bdbiosciences.com)
May participate in antigen-induced T-cell activation. (lu.se)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)

ES-E14TG2a cells (ATCC CRL-1821) were cultured, fixed, and stained with Alexa Fluor® 488 Mouse anti-SSEA-1 (pseudo-colored green) according to the Recommended Assay Procedure. (bdbiosciences.com)
2. Dilute the recombinant mouse Cathepsin B to 0.05 μg/ml in assay buffer (25 mM MES, pH 5.0). (biolegend.com)

Verma said these cells can be used either for cloning or to grow eggs or sperm in a lab. (scientificamerican.com)
The cells could also be stored in cryopreservation for future use when eggs and sperm are too difficult to collect or freeze. (scientificamerican.com)

ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)
Expression of AND-34 regulates epithelial cell growth pattern, motility, and growth factor dependence. (molvis.org)
ALiCE® is a radically different approach to cell-free expression and a kit unlike any others. (amsbio.com)
Our results suggest that although trisomy 8 cells are in a pro-apoptotic state, they are checked by the enhanced expression of anti-apoptotic signals which provide them with their proliferative advantage. (who.int)

This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. (origene.com)
ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)

The bone marrow contains various populations of skeletal stem cells (SSCs) in the stromal compartment, which are important regulators of bone formation. (nature.com)
The term "transitional" emphasizes the unique feature of these cells that are particularly abundant in the young bone marrow. (nature.com)
We investigated the origin of SF3B1 mutations within the bone marrow hematopoietic stem and progenitor cell compartments in patients with MDS-RS. (nih.gov)
15]. The percentage of bone common of which are trisomy 8, mono- marrow blast cells for estimation of the Myelodysplastic syndrome (MDS) is somy 7 and 5q- [11]. (who.int)

The thymus plays a central role in the development of the body's immune system as it is the main site in which T cells are generated. (eurostemcell.org)

Affordable & consistent controls from 50 tumor cell lines, ideal for IHC, in situ hybridization & NGS applications. (amsbio.com)

1. Dilute the recombinant mouse Cathepsin B to 10 μg/ml in activation buffer (25 mM MES, 1 mM DTT, pH 5.0) and incubate the protein at 25°C for 3 min. (biolegend.com)
Full length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell. (origene.com)

Anatomy56

Organisms15

Diseases12

Chemicals and Drugs56

Analytical, Diagnostic and Therapeutic Techniques and Equipment61

Phenomena and Processes57

Disciplines and Occupations4

Technology, Industry, Agriculture1

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Named Groups1

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Differentiation15

Embryo10

Somatic11

Therapeutic10

Tissue14

Progenitor cells6

Humans10

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19972

Embryonic stem29

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Proteins3

Monoclonal1

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