Stem cells epithelial cells. Medical search. Frequent questions

The formation of CSCs is highly associated with cancer cell epithelial-mesenchymal transition (EMT), which contributes to the plasticity of cancer cells and comprises an initial step for metastasis. (ku.edu)
Snail-mediated epithelial-mesenchymal transition (EMT) process plays a fundamental role in facilitating pancreatic ductal adenocarcinoma (PDAC) stemness and metastasis. (jcancer.org)
G. Shieh, W. Yang, C. Wu and A. Shiau, Lactate promoting cancer stem cell phenotype and inducing epithelial-mesenchymal transition, Urological Science (2016). (scipedia.com)
In this study, we explored the role of lactate shuttle induced by HK2 in cancer stem cell formation and epithelial-mesenchymal transition (EMT) between bladder cancer cells in vitro and in vivo. (scipedia.com)
The effects of IL15 secreted by GCMSCs on GC development were evaluated by detecting the stemness, epithelial-mesenchymal transition (EMT), and migration abilities of GC cell lines . (bvsalud.org)
Upon its transition to the precancerous Barrett's esophagus epithelium, however, a small dedicated stem cell compartment appears that supports proliferation of the epithelium while concomitantly evidence for a stochastically dividing compartment contributing to the maintenance of the tissue disappears. (wikipedia.org)
The epithelial-mesenchymal transition (EMT) is a key process in successful execution of many steps such as the invasion and metastasis for cancer cells. (elsevierpure.com)
TG2 expression correlated with tumour advancement and expression of markers of epithelial-mesenchymal transition (EMT). (oncotarget.com)
The protein kinase C (PKC) activator phorbol 12-myristate 13-acetate (PMA) induces transition of the epithelial MCF-7 cell line to a mesenchymal phenotype. (edu.au)
We profiled the transcriptome changes associated with the epithelial to mesenchymal transition and those that occurred in the CSC subset. (edu.au)

These glands uniquely express Shh and TFF2 as well as other developmental genes known to reside in progenitor stem cell niches. (nebraska.edu)
SA1] The experiments proposed in this aim will determine the role of PDG as an epithelial progenitor stem cell niche responsible for epithelial renewal and regeneration. (nebraska.edu)
We propose to use a fate mapping, in vivo lineage tagging strategy specifically tagging the PDG compartment, in combination with a well-characterized model of acute and chronic pancreatic injury, to confirm that these compartments contain epithelial progenitor cells. (nebraska.edu)
At least two populations of epithelial stem/progenitor cells give rise to the lung anlage, comprising the laryngo-tracheal complex versus the distal lung below the first bronchial bifurcation. (nih.gov)
Strategies to enhance endogenous alveolar epithelial repair could include protection of epithelial progenitors from injury and/or stimulation of endogenous progenitor cell function. (nih.gov)
Alternatively, exogenous stem/progenitor cells can be delivered into the lung either intravenously, intratracheally, or by direct injection. (nih.gov)
Sources of exogenous stem/progenitor cells that are currently under evaluation in the context of acute lung injury repair include embryonic stem cells, bone marrow- or fat-derived mesenchymal stem cells, circulating endothelial progenitors, and, recently, amniotic fluid stem/progenitor cells. (nih.gov)
Further work will be needed to translate stem/progenitor cell therapy for the lung. (nih.gov)
Recent advances in isolating epithelial progenitor cells and stromal cells, as well as defining niche factors important for lung development, have led to the establishment of an in vitro 3D lung culture system: pulmonary (lung) organoids. (stemcell.com)
Recent progress isolating lung epithelial progenitor and stromal cells, as well as defining stem cell niche factors that are important for lung development, has led to the establishment of an in vitro three-dimensional (3D) culture system: lung organoids 2 , also known as airway organoids or pulmonary organoids. (stemcell.com)
Identification of multipotent luminal progenitor cells in human prostate organoid cultures. (nature.com)
To investigate the involvement of stem cells in Epstein-Barr virus infection-related nasopharyngeal carcinoma (NPC), we used double immunofluorescence staining to examine several cancer stem/progenitor cell markers (CD44v6, CD24, and ALDH1A1) in NPC tissues and NPC cell lines. (hindawi.com)
Inflammation-associated tissue injury may activate stem/progenitor cells, and mutagenic stimuli from inflammation can accumulate multiple mutations and epigenetic changes in stem/progenitor cells [ 3 , 10 ]. (hindawi.com)
Victoria Ptasinski defended her Ph.D. thesis 'Alveolar Progenitor Cells in Lung Damage and Regeneration in Pulmonary Fibrosis' on Monday, November 27, 2023. (lu.se)
Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)
The scientists at the University of California San Diego report on a method of removing cataracts while preserving epithelial stem/progenitor cells (LECs), which have an important role in lens regeneration. (medscape.com)

In this study, we investigated the maintenance of hESCs and their differentiation into RPE using Synthemax II-SC, which is a novel, synthetic animal-derived component-free, RGD peptide-containing copolymer compliant with good manufacturing practices designed for xeno-free stem cell culture. (ca.gov)
In this study, we show that increased expression of Runt related transcription factor 1 (RUNX1) maintains undifferentiated state of CSC-like SP cells through upregulation of inhibitors of DNA binding/differentiation genes (ID1 and ID3) in late cisplatin-paclitaxel resistant cells. (techscience.com)
Intriguingly RUNX1 inhibition not only induces CSC differentiation but also downregulates anti-apoptotic protein BCL2 in both SP and NSP cells and potentiates cytotoxic effects of Cisplatin-Paclitaxel in chemoresistant EOC cells. (techscience.com)
Cancer stem cells, as a source in cancer invasion and metastasis, possess powerful potential of differentiation. (bmj.com)
Methods Spheroid culture with serum-free medium was used for enriching ovarian cancer stem cell-like cells (CSC-LCs) from 3AO cell line, and a medium with 10% fetal bovine serum was used to induce the differentiation of CSC-LCs. (bmj.com)
IQGAP1 expression was decreased in ovarian CSC-LCs compared with parental 3AO cells, but increased de novo during the differentiation of CSC-LCs. (bmj.com)
Conclusions Increased IQGAP1 expression during the differentiation of CSC-LCs is involved in an aggressive cell behavior, which may contribute to metastasis of ovarian cancer. (bmj.com)
CSCs may generate tumors through the stem cell processes of self-renewal and differentiation into multiple cell types. (wikipedia.org)
In different tumor subtypes, cells within the tumor population exhibit functional heterogeneity and tumors are formed from cells with various proliferative and differentiation capacities. (wikipedia.org)
In this study we investigated the role of epigenetic regulation of CD133 in epithelial differentiation and cancer. (biomedcentral.com)
This indicates that CD133 expression is not altered in prostate cancer and it is consistent with an important role for CD133 in the maintenance of the hierarchical cell differentiation patterns in cancer. (biomedcentral.com)
As in normal tissues, a small percentage of cancer cells can persist and initiate new tumour growth (tumour-initiating cells or cancer stem cells - CSCs), while most cells proceed to terminal differentiation [ 6 ]. (biomedcentral.com)
A long-term multi-organ co-culture system coupled with quantitative imaging to visualize blood cell cycle progression, analyze polarized blood cell mitosis, and track blood cell differentiation kinetics during Drosophila hematopoiesis in homeostatic condition and following infection. (elifesciences.org)
The authors sought to identify mechanisms driving the differentiation of mucus cells during the bronchiolisation process. (bmj.com)
Methods Pathways governing airway mucus cell differentiation include SRY (sex determining region Y)-box 2 (SOX2), Notch, forkhead box A3(FOXA3)/SAM pointed domain containing ETS transcription factor (SPDEF), epidermal growth factor (EGF) and the EGF-related neuregulins NRG1α and NRG1β. (bmj.com)
Results In IPF, the bronchiolised and enlarged distal airspaces stained for SOX2 are consistent with epithelial differentiation characteristic of conducting airway epithelium. (bmj.com)
In this work, pathways governing mucus cell differentiation were investigated in lung tissues from patients with IPF and other chronic pulmonary disorders. (bmj.com)
Neuregulin1α, which drives mucus cell differentiation in vitro, was expressed in normal airway submucosal glands and in lungs from patients with IPF. (bmj.com)
Activation of abnormal respiratory epithelial differentiation programs may contribute to the expression of MUC5B and bronchiolisation of the distal lung, a salient feature of IPF. (bmj.com)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
Recent advances in live imaging and genetics of mammalian division, movement and cell differentiation leading to development which integrate observations of biochemical tissue formation [14 ]. (lu.se)

Signals that regulate epithelial regeneration upon stem cell injury are largely unknown. (nature.com)
Upon Lgr5 + cell depletion, these cells are recruited to contribute to crypt regeneration. (nature.com)
Chemical injury induced by DSS leads to a loss of both Lgr5 + cells and Axin2 + cells and epithelial regeneration is driven by Axin2 − cells, including differentiated Krt20 + surface enterocytes. (nature.com)
Regeneration requires stromal Rspo3, which is present at increased levels upon injury and reprograms Lgr5 − but Lgr4 + differentiated cells. (nature.com)
This Rspo3-driven regeneration program is supported by injury-induced stromal remodeling, and is essential for epithelial recovery. (nature.com)
Thus, we find that endogenous Rspo3 signaling is a critical determinant of cellular fate within the crypt and stimulates rapid recruitment of differentiated cells for epithelial wound healing and crypt regeneration through induction of Wnt signaling. (nature.com)
To date little is known about these somatic stem cell niches or their contribution to pancreatic epithelial regeneration and cancer. (nebraska.edu)
This proposal is focused on characterizing the role of the PDG compartment in epithelial regeneration and carcinogenesis. (nebraska.edu)
In order to better characterize this two stem cell population we propose a developmental sub-aim to evaluate the transcriptome by mRNA-Seq from minimal (10-cell) samples in order to identify unique markers for these cells, as well as understand key pathways that regulate this compartment during regeneration and inflammatory metaplasia. (nebraska.edu)
Residual pools of adult stem cells are hypothesized to be the source of lung regeneration and repair. (nih.gov)
Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)
Commenting on the study in a statement, Dr Dusko Ilic, reader in stem cell science at King's College London, says: "The basic science research led to the hypothesis that preserving and stimulating autologous stem cells in the eye might promote regeneration of a surgically removed lens. (medscape.com)
and stem cell regeneration. (lu.se)

These pools have been located within the basal layer of the upper airways, within or near pulmonary neuroendocrine cell rests, at the bronchoalveolar junction as well as within the alveolar epithelial surface. (nih.gov)
The human airway, from the nasal passage to the alveolar sacs, is covered with a continuous epithelial sheet that differs in morphology and cellular composition between the conducting and respiratory zones. (stemcell.com)
The alveolar epithelium is lined with type I and II alveolar epithelial cells (AECs). (stemcell.com)
In our latest work, we developed longitudinal timelapse imaging of intact lung tissue in vivo and ex vivo and discovered alveolar stem cell migration between alveoli as a new cellular mechanism required for repair (Chioccioli et al. (findapostdoc.com)
We have multiple NIH grants for suite of projects that together aim to define the molecular and cellular mechanisms that drive alveolar epithelial cell repair in the live mouse. (findapostdoc.com)
We developed a model of the aberrant epithelial reprogramming observed in IPF using alveolar organoids derived from human-induced pluripotent stem cells stimulated with a cocktail of pro-fibrotic and inflammatory cytokines. (lu.se)
Deconvolution of RNA-seq data of alveolar organoids indicated that the fibrosis cocktail rapidly increased the proportion of transitional cell types including the KRT5-/KRT17+ aberrant basaloid phenotype recently identified in the lungs of. (lu.se)

Tumor cells can extrude and shuttle lactate to neighboring cancer cells, adjacent stromal cells, and vascular endothelial cells to induce signaling molecular change. (scipedia.com)
Several studies show that mesenchymal stem cells (MSCs) homing to tumors not only provide the microenvironment for tumor cells but also promote tumor growth and metastasis . (bvsalud.org)
Essentially this theory proposes that all cells have the ability to be tumorigenic making all tumor cells equipotent with the ability to self-renew or differentiate, leading to tumor heterogeneity while others can differentiate into non-CSCs The cell's potential can be influenced by unpredicted genetic or epigenetic factors, resulting in phenotypically diverse cells in both the tumorigenic and non-tumorigenic cells that compose the tumor. (wikipedia.org)
According to the "stochastic model" (or "clonal evolution model") every cancer cell in a tumor could gain the ability to self-renew and differentiate to the numerous and heterogeneous lineages of cancer cells that compromise a tumor These mutations could progressively accumulate and enhance the resistance and fitness of cells that allow them to outcompete other tumor cells, better known as the somatic evolution model. (wikipedia.org)
Cancer stem-like cells (CSCs) are associated with cancer progression, metastasis, and recurrence, and may also represent a subset of circulating tumor cells (CTCs). (biorxiv.org)

The EasySep™ Mouse Epithelial Cell Enrichment Kit II is designed to isolate epithelial cells from freshly dissociated mouse mammary tissues by immunomagnetic negative selection. (stemcell.com)
Epithelial ovarian cancers (EOC) are a complex group of tumors that arise from multiple different precursor tissues, and the most common EOC subtype (high-grade serous) is now thought to originate in both the ovary and the fallopian tube. (ocrahope.org)
Hence, at least for certain neoplastic tissues, dedicated stem cell compartments maintain and enlarge the size of the transformed compartment The cancer stem cell model, also known as the Hierarchical Model proposes that tumors are hierarchically organized (CSCs lying at the apex (Fig. 3). (wikipedia.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
CD133 is a marker for adult stem cells in various tissues and tumour types. (biomedcentral.com)
It plays a crucial role in preserving the hierarchical structure of tissues and is involved in maintaining stemness and fate determination of adult stem cells [ 2 , 3 ]. (biomedcentral.com)
Indeed, CD133 + cells from human primary tissues represent a very small subpopulation (0.1-3.0% of the total prostatic epithelium) located in the basal compartment. (biomedcentral.com)
Adult stem cell-based organoid technology is a versatile tool for the generation and long-term maintenance of near-native 3D epithelial tissues in vitro. (nature.com)
Here, we provide an overview of the protocols used by different groups to establish organoids from various epithelial tissues and cancers, plus the different protocols subsequently used to test the in vitro therapy sensitivity of these patient-derived organoids. (nature.com)
The staining intensity of 8-nitroguanine was significantly higher in cancer cells and inflammatory cells in the stroma of NPC tissues than in chronic nasopharyngitis tissues. (hindawi.com)
Expression levels of CD44v6 and ALDH1A1 were significantly increased in cancer cells of primary NPC specimens in comparison to chronic nasopharyngitis tissues. (hindawi.com)
8-Nitroguanine was detected in both CD44v6- and ALDH1A1-positive stem cells in NPC tissues. (hindawi.com)
Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)

The animal survival and lung metastasis were assessed in a mouse subcutaneous model using TSGH8301 cells with HK2-overexpression clones. (scipedia.com)
Image shows the expression of an adult stem cell signature marker (dark brown) in less aggressive lung cancer cells (left) and highly aggressive lung cancer cells (right). (ucla.edu)
The lung is a complex organ composed of many types of epithelial cells, immune cells, endothelial cells and stromal cells. (stemcell.com)
We combine powerful genome-wide 'omics approaches, genetic mouse models, in vivo and ex vivo live imaging, advanced microscopy and human lung slice culture to uncover fundamental mechanisms of epithelial stem cell-based repair in the lung. (findapostdoc.com)
Interest in the lung epithelial stem cell field. (findapostdoc.com)
Induction of stem-like cells with malignant properties by chronic exposure of human lung epithelial cells to single-walled carbon nanotube s. (cdc.gov)
Methods: Non-tumorigenic human lung epithelial cells were chronically exposed to well-dispersed SWCNT for a period of 6 months at the physiologically relevant concentration of 0.02 mu g/cm(2) surface area dose. (cdc.gov)
Non-small cell lung cancer cells served as a positive control. (cdc.gov)
Results: We demonstrated for the first time the existence of CSC-like cells in all clones of chronic SWCNT-exposed lung epithelial cells. (cdc.gov)
These CSC-like cells, in contrary to their non-CSC counterpart, possessed all biological features of lung CSC that are central to irreversible malignant transformation, self-renewal, aggressive cancer behaviors, and in vivo tumorigenesis. (cdc.gov)
In vitro generation of human pluripotent stem cell derived lung organoids. (nature.com)
In doing so, I have turned my attention toward the cells that form the barrier of the lung facing the outer environment. (lu.se)

One of the main characteristics of stem cells is their ability to self-renew to maintain a tissue for a lifetime. (whiterose.ac.uk)
Little is know about normal tissue stem cells in these organs, but we are able to selectively culture these cells in our laboratory. (ocrahope.org)
To induce central T-cell tolerance, medullary thymic epithelial cells (mTEC) collectively express most protein-coding genes, thereby presenting an extensive library of tissue-restricted antigens (TRAs). (ox.ac.uk)
Unexpectedly, 50 modules of genes were robustly defined each showing patterns of co-expression within individual cells, which were mainly not explicable by chromosomal location, biological pathway or tissue specificity. (ox.ac.uk)
An allogeneic source of transplantable cells for use in total bilateral disease can be obtained from culture of donated cadaveric corneal tissue. (liverpool.ac.uk)
Tissue stem cells are restricted to the α 2 β 1 integrin hi population, have a high colony-forming ability and proliferative potential, and are able to regenerate fully differentiated prostate epithelium in vivo. (biomedcentral.com)
Autofluorescence imaging permits label-free cell type identification in live airway tissue and reveals the dynamic formation of airway secretory cell associated antigen passages. (elifesciences.org)
Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
Recent news of an impending clinical cell transplantation trial in Parkinson's disease using parthenogenetic stem cells as a source of donor tissue have raised hopes in the patient community and sparked discussion in the research community. (lu.se)
of the most critical events which lead to tissue patterning involves mechanisms going beyond single cells. (lu.se)

Biologically indeterminate yet ordered promiscuous gene expression in single medullary thymic epithelial cells. (ox.ac.uk)
Zhao Y.(2011).Mouse embryonic stem cell-derived thymic epithelial cell progenitors enhance T-cell reconstitution after allogeneic bone marrow transplantation. (ioz.ac.cn)

This has been studied mainly in the small intestine, where different cell types such as enterocyte progenitors 4 , more differentiated secretory Bmi1 + cells 3 , or even fully differentiated Paneth cells can repopulate crypts upon injury. (nature.com)

Lgr5 is a well-established marker of these basal stem cells in the gastrointestinal tract 1 and lineage tracing experiments have demonstrated that Lgr5 + cells give rise to clonal cell populations establishing entire colonic crypts 2 . (nature.com)
The major cell types of the large airway epithelium are goblet cells that produce and secrete mucus, ciliated cells that promote mucus motility through coordinated movement of their apical cilia, and basal cells that line the basement membrane and do not contact the apical surface of the epithelium. (stemcell.com)
Moreover, differential methylation of CD133 was absent from both benign or malignant CD133 + /α 2 β 1 integrin hi prostate (stem) cells, when compared to CD133 - /α 2 β 1 integrin hi (transit amplifying) cells or CD133 - /α 2 β 1 integrin low (basal committed) cells, selected from primary epithelial cultures. (biomedcentral.com)
The corneal epithelium is maintained by a population of stem cells known as limbal stem cells (LSCs) due to their location in the basal layer of the outer border of the cornea known as the limbus. (ncl.ac.uk)
Células madre somáticas adultas presentes en la capa epitelial basal del LIMBO DE LA CÓRNEA. (bvsalud.org)
Adult somatic stem cells found in the basal epithelial layer of the LIMBUS CORNEAE. (bvsalud.org)

For corneal stem cells these are located in intricate microenvironments or niches situated within areas of the limbal region known as the Palisades of Vogt. (whiterose.ac.uk)
We performed a controlled multicenter study to examine the feasibility, safety, and efficacy of allogeneic corneal epithelial stem cells in the treatment of bilateral LSCD. (liverpool.ac.uk)
Patients were randomized to receive corneal epithelial stem cells cultured on amniotic membrane (AM): investigational medicinal product (IMP) or control AM only. (liverpool.ac.uk)
This first randomized control trial of allogeneic corneal epithelial stem cells in severe bilateral LSCD demonstrates the feasibility and safety of this approach. (liverpool.ac.uk)
05). This study demonstrates that transplantation of autologous limbal epithelial stem cells cultured on HAM without the use of non-human animal cells or products is a safe and effective method of reconstructing the corneal surface and restoring useful vision in patients with unilateral total LSCD. (ncl.ac.uk)
However, treatment with various approaches, including exogenous WNT5A methylation inhibitors and a nanoconjugate that inhibits WNT5A suppression, improved corneal epithelial wound healing as well as expression of the limbic epithelial stem cells. (medscape.com)
Overall, Wnt-5a is a new corneal epithelial wound healing stimulator that can be targeted to improve wound healing and stem cells in the diabetic cornea," write Ruchi Shah, PhD, of the Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, California, and colleagues. (medscape.com)
The finding of Wnt-5a as a new diabetic corneal marker regulating wound healing and stem cell function may have implications for other diabetes complications involving impaired wound healing, including diabetic foot ulcers, as they share similar neurovascular, sensory, and immunological compromise with diabetic eye disease, Shah and colleagues say. (medscape.com)
The central corneal endothelium is removed, and the use of topical rho kinase inhibitors speeds the migration of peripheral corneal endothelium cells to fill the defect. (msdmanuals.com)
The migrated cells reduce the corneal stromal edema and vision improves. (msdmanuals.com)

Autologous limbal stem cell transplantation is effective in unilateral or partial bilateral disease but not applicable in total bilateral disease. (liverpool.ac.uk)
Treatment of limbal stem cell deficiency (LSCD) has been achieved with transplantation of ex vivo expanded LSCs taken from a small biopsy of limbus. (ncl.ac.uk)
Based on discussions held by a global collaborative initiative on translation of stem cell therapy in Parkinson's disease, we have identified a set of key questions that we believe should be addressed ahead of every clinical stem cell-based transplantation trial in this disorder. (lu.se)

Conditionally reprogrammed physiological endometrial epithelial cells provide a novel strategy in IUA clinics in a personalized or generalized manner and also serve as a physiological model to explore biology of endometrial epithelial cells and mechanisms of IUA. (biomedcentral.com)
Education & qualifications Applicants should have, or be close to obtaining, a PhD in a relevant field such as genetics, cell biology, molecular biology, developmental biology, or related areas. (findapostdoc.com)
This highlights the crucial choice of cell model systems when studying epigenetic control in cancer biology and stem cell biology. (biomedcentral.com)
However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)

Defined culture of human embryonic stem cells and xeno-free derivation of retinal pigmented epithelial cells on a novel, synthetic substrate. (ca.gov)
Human embryonic stem cells (hESCs) can generate an unlimited source of RPE for cellular therapies, and clinical trials have been initiated. (ca.gov)
TATA-box binding protein is not required for RNA Polymerase II transcription in mouse embryonic stem cells. (elifesciences.org)

Our data showed that knockdown of HuR in pancreatic cancer cells significantly inhibited cell migration, invasion, and stem-like features. (ku.edu)
Restoration of HuR in HuR deleted (KO) pancreatic cancer cells enhanced migration and enriched CSC population. (ku.edu)
As an HuR/mRNA disruptor, KH-3 treatment successfully mimicked the effects of HuR knockdown in pancreatic cancer cell lines: KH-3 significantly inhibited pancreatic cancer cell migration, invasion, tumor sphere formation and EMT. (ku.edu)
In this study we introduced micropockets into biodegradable microfabricated membranes and explored the potential contribution of these structures to limbal cell migration and their ability to deliver cells to a 3D cornea model. (whiterose.ac.uk)
Results indicate that the micropockets enhance the migration of cells from limbal explants and cells transfer readily from the membranes to the ex vivo cornea model. (whiterose.ac.uk)
We found that IL15 derived from GCMSCs enhanced stemness, induced EMT and promoted migration of GC cell lines . (bvsalud.org)
Cell migration and invasion were assessed using wound-healing assay and modified Boyden chamber assay, respectively. (elsevierpure.com)
Introduction of Snail induced EMT properties in HNSCC cells and enhanced cell migration and invasion. (elsevierpure.com)
CSC-like cells were isolated using fluorescence-activated cell sorting and were assessed for aggressive behaviors, including acquired apoptosis resistance and increased cell migration and invasion in vitro, and tumor-initiating capability in vivo. (cdc.gov)
We demonstrate that CD117+ cells display increased proliferation and migration. (biorxiv.org)
Role of DARPP-32 in Breast Cancer Cell Signalling and Migration. (lu.se)
Involvement of eicosanoid signalling in epithelial cell migration. (lu.se)

This constant replacement of the epithelium is driven by cells located in the base of colonic crypts, which constantly divide and give rise to the various differentiated cell types. (nature.com)
In the bronchioles, the cuboidal epithelium contains secretory club cells and fewer ciliated cells than in more proximal airway regions. (stemcell.com)
However, available therapies do not specifically target the epithelium and human models of fibrotic epithelial damage with suitability for drug discovery are lacking. (lu.se)

In this study we will isolate and characterize stem cells from normal ovaries and fallopian tubes and compare the molecular profiles to stem cells from ovarian cancers, to identify common markers between the groups. (ocrahope.org)
Immunofluorescence was for detecting the stem markers OCT4 and SOX2. (bmj.com)
Stable HK2 overexpression induced cell proliferation, and showed morphologic changes with gain of cancer stem cell markers. (scipedia.com)
In response to lactate exposure, nuclear translocation of NF-κB phosphorylation and Twist1 as well as mesenchymal markers was promoted in human bladder cancer cells. (scipedia.com)
A subset of the resulting mesenchymal cells has surface markers characteristics of a cancer stem cell (CSC) population. (edu.au)
These cells also displayed aberrant stem cell markers, notably Nanog, SOX-2, SOX-17 and E-cadherin. (cdc.gov)
Furthermore, we identified specific stem cell surface markers CD24(low) and CD133(high) that are associated with SWCNT-induced CSC formation and tumorigenesis. (cdc.gov)
In conclusion, CD44v6 and ALDH1A1 are candidate stem cell markers for NPC, and the increased formation of DNA lesions by inflammation may result in the mutation of stem cells, leading to tumor development in NPC. (hindawi.com)

Cells were treated with DNA demethylating agents and HDAC inhibitors. (biomedcentral.com)
Condensed chromatin was associated with CD133 downregulation in all of the cell lines, and treatment with HDAC inhibitors resulted in CD133 re-expression in both cell lines and primary samples. (biomedcentral.com)
Further, in cell lines, CD117 activation by SCF promotes faster proliferation and invasiveness, while blocking CD117 activation with tyrosine kinase inhibitors (TKIs) decreased progression in a context-dependent manner. (biorxiv.org)

The intricate molecular mechanisms involved in the regenerative process of the normal intestine and the identity of putative somatic intestinal stem cells have become clear. (mdpi.com)
Sigalling and trafficking of the cysteinyl leukotriene receptors in intestinal epithelial cells. (lu.se)
Regulation of survival and proliferationof human intestinal epithelial cells by leukotriene D4. (lu.se)

Head and neck squamous cell carcinoma (HNSCC) is known to have a poor prognosis. (elsevierpure.com)
We also provide an in-depth protocol for the generation of head and neck squamous cell carcinoma organoids and their subsequent use in semi-automated therapy screens. (nature.com)

In both 2D and 3D cultures, stemness marker gene expression is higher in CD117+ cells. (biorxiv.org)

Conclusions: Our findings provide new and compelling evidence for the acquisition of CSC-like cells induced by chronic SWCNT exposure, which are likely to be a major driving force for SWCNT tumorigenesis. (cdc.gov)
These cells are defined as tumor-initiating cells or rare cells with indefinite potential for self-renewal that drives tumorigenesis [ 15 ]. (hindawi.com)

Primary endometrial epithelial cells for cell therapy are largely hampered with the extremely limited proliferation capacity of uterine epithelial cells. (biomedcentral.com)
Effects of lactate exposure on cell proliferation, morphologic change and cancer stem cell phenotype were analyzed in human bladder cancer cells. (scipedia.com)

The HK2 expression was significantly higher in bladder cancers compared with normal cells. (scipedia.com)
High HK2 expression in bladder cancers induced oversecretion of lactate, which was associated with metastatic behaviors through the cancer stem cell formation, EMT promotion and nuclear translocation of phosphorylated NF-κB and Twist1. (scipedia.com)
Cancer stem cells (CSCs) are cancer cells (found within tumors or hematological cancers) that possess characteristics associated with normal stem cells, specifically the ability to give rise to all cell types found in a particular cancer sample. (wikipedia.org)

In vitro and in viv strategies are also proposed to determine if these cells have multipotential capacity. (nebraska.edu)

Scaffolding IQ domain GTPase-activating protein 1 (IQGAP1) plays a key role in the invasion and metastasis of cancer cells, but IQGAP1's role in cancer stem cells including ovarian cancer was unclear. (bmj.com)
Our data proved that the high concentration of IL15 in tumor microenvironment , which was mainly secreted by GCMSCs, may contribute to tumor cell metastasis and offer a new opportunity to develop effective therapeutics for intercepting tumor progression. (bvsalud.org)
Such cells are hypothesized to persist in tumors as a distinct population and cause relapse and metastasis by giving rise to new tumors. (wikipedia.org)

Some adult stem cell niches have already been described, but the majority of them remain unclear, including the dental pulp stem cell niches. (bvsalud.org)

Additionally, we are performing biochemical characterization of this molecule to determine how it may affect cell adhesion and other cell functions. (cincinnatichildrens.org)
Little is known about molecular mecha- desmosomal glycoproteins, resulting in the nisms affecting mast cell and T lympho- loss of keratinocyte cell-cell adhesion [ 1 ]. (who.int)

Our preliminary data suggests that this compartment has at least two populations of stem cells, label retaining cells (LRC) and transient amplifying cells (TA). (nebraska.edu)

Overexpression of miR-30b led to reduced expression of mesenchymal marker N-cadherin and the upregulation of epithelial marker E-cadherin. (jcancer.org)
β-Catenin and TG2 was also upregulated in SW620 spheroid cells enriched with cancer stem cell marker CD44 and TG2 inhibition/knockdown reduced the spheroid forming potential of SW620 cells. (oncotarget.com)

Although cultured limbal epithelial cells have been used to regenerate scarred corneas for more than 15 years, the culture strategies do not deliver cells under the physiological conditions they experience in vivo. (whiterose.ac.uk)
We propose that normal stem cells, that usually regenerate the epithelia linings of the ovary and fallopian tube, may be cells of origin for EOC. (ocrahope.org)
During menses, the epithelial cells re-epithelialize the exposed surface and regenerate the new functionalis under the control of estrogen, while the glands remain in the basalis layer [ 2 ]. (biomedcentral.com)
Unlike the liver and skin, where cells divide to create new daughter cells and regenerate the organ, cells in the proximal tubules of the kidney are mitotically quiescent - they do not divide to create new cells. (scitechdaily.com)
In most scenarios, if kidney cells are severely injured, they will die, and they cannot regenerate," said Zheng, a Distinguished Chair in Natural Sciences and Mathematics. (scitechdaily.com)

Crosstalk between colorectal cancer cells and tumour-associated macrophages. (lu.se)

We earlier reported enrichment of CSC-like side population (SP) with increasing resistance towards Cisplatin and Paclitaxel either alone or in combination in epithelial ovarian cancer (EOC) cells. (techscience.com)

We identify two populations of cells with active Wnt signaling: highly proliferative Lgr5 + /Axin2 + cells, as well as secretory Lgr5 − /Axin2 + cells. (nature.com)

We demonstrate that while Rspo3 from myofibroblasts maintains colonic Lgr5 + cells during homeostasis, during injury its main function is not to maintain Lgr5 + cells but to interact with more differentiated cells that express Lgr4 but not Lgr5 and are able to regain expression of Wnt target genes and generate new crypts. (nature.com)
To generate models of early cancer development we will introduce genetic changes into the stem cells, selectively altering genes that are common deregulated in high-grade serous EOCs. (ocrahope.org)

Recently, it has been shown that Snail-induced EMT could induce a cancer stem cell (CSC)-like phenotype in a number of tumor types. (elsevierpure.com)

Pancreatic cancer is enriched with cancer stem cells (CSCs), which contribute to its high metastatic tendency and resistance to treatments. (ku.edu)
Data demonstrated that Pao and Rau preferentially inhibited the viability of pancreatic CSC population, compared with the whole cancer cell population. (ku.edu)
The data demonstrated that inhibition of HuR destabilized SNAI1 mRNA, and subsequently reduced Snail protein levels, resulting in the EMT and CSCs inhibition in pancreatic cancer cells. (ku.edu)
In the present study, we revealed that microRNA-30 (miR-30) members, especially miR-30b, were remarkably downregulated in triple-positive (CD24 + , CD44 + , EpCAM + ) pancreatic cancer stem cells (PCSCs). (jcancer.org)
Our laboratory has recently discovered a novel epithelial stem cell compartment, Pancreatic Duct Glands (PDG). (nebraska.edu)

We hypothesized that the acquisition of cancer stem cells (CSC), a subpopulation that drive tumor initiation and progression, may contribute to CNT carcinogenesis. (cdc.gov)
During inflammation, reactive oxygen species (ROS) and reactive nitrogen species (RNS) are generated from inflammatory cells and are considered to play key roles in carcinogenesis [ 3 ]. (hindawi.com)
Recently, evidence has accumulated showing that stem cells are involved in inflammation-related carcinogenesis. (hindawi.com)

It often has multiple types of cells mirroring their counterpart in the body along with constituents of native extracellular matrix (ECM) and is typically miniature or micro-scale in size. (stemcell.com)
TGFβ1 expression in CRC cells, and its level in the cell medium and extracellular matrix was increased in primary tumour CRCs overexpressing TG2 and could regulate TG2 expression and EMT by both canonical (RKO) and non-canonical (RKO and SW480) signalling. (oncotarget.com)
We found that epithelial reprogramming and extracellular matrix (ECM) production persisted after removal of the fibrosis cocktail. (lu.se)

Human colon cancer cell lines (CRCs) RKO, SW480 and SW620 were investigated for TG2 involvement in tumour advancement and aggression. (oncotarget.com)

We now seek to explore how different signals and cell-cell interactions modulate this and other repair mechanisms, and how these go awry in disease. (findapostdoc.com)

Kretzschmar, K. & Clevers, H. Organoids: modeling development and the stem cell niche in a dish. (nature.com)
Stem cell niche. (bvsalud.org)

Within the cancer population of the tumors there are cancer stem cells (CSC) that are tumorigenic cells and are biologically distinct from other subpopulations They have two defining features: their long-term ability to self-renew and their capacity to differentiate into progeny that is non-tumorigenic but still contributes to the growth of the tumor. (wikipedia.org)

Anatomy73

Organisms14

Diseases6

Chemicals and Drugs57

Analytical, Diagnostic and Therapeutic Techniques and Equipment48

Phenomena and Processes58

Disciplines and Occupations4

Technology, Industry, Agriculture1

Information Science3

Transition10

Progenitor16

Differentiation21

Regeneration14

Alveolar7

Tumor cells5

Tissues13

Lung12

Tissue10

Thymic epithelial2

Progenitors1

Basal6

Corneal10

Transplantation3

Biology4

Embryonic3

Migration12

Epithelium3

Markers8

Inhibitors3

Intestinal3

Head and Neck Squamous Cell Carc2

Stemness1

Tumorigenesis2

Proliferation2

Cancers3

Vitro1

Metastasis3

Adult1

Adhesion2

Least two populations1

Marker2

Regenerate5

Colorectal1

Ovarian cancer1

Populations1

Genes2

Phenotype1

Pancreatic5

Carcinogenesis3

Extracellular3

Cancer cell1

Mechanisms1

Niche2

Tumors1