Stem cells cells differentiate. Medical search. Frequent questions

Human mesenchymal stem cells (hMSCs) have emerged as a leading candidate in cellular therapies due to their unique properties. (selectscience.net)
Because human mesenchymal stem cells are known to be sensitive to their mechanical environments, we investigated the mechanotransductive potential of Descemet membrane-like microtopography (DLT) to differentiate human mesenchymal stem cells into CEC-like cells. (lww.com)
Master molds with inverted DLT were produced by 2-photon lithography (2-PL). To measure the mechanotransductive potential of DLT, mesenchymal stem cells were cultivated on silicone or collagen imprints with DLT. (lww.com)
Adult human bone marrow (BM) is the major source of mesenchymal stem cells (MSC) for cell-based therapies. (journalcra.com)
This study focuses to identify, isolate and characterize the mesenchymal stem cells that are believed to be present in BM. (journalcra.com)
And hence, this study suggests that human bone marrow is a potential source of mesenchymal stem cells that could be differentiated in to ccardiomyocytes and used for cell based therapies for treating various cardiovascular diseases. (journalcra.com)
Here, we investigated whether our recently-established human tonsil-derived mesenchymal stem cells (TMSC) restore invivo parathyroid cell function in rats with parathyroidectomy (PTX). (ewha.ac.kr)
However, mesenchymal stem cells have shown to be effective against multiple sclerosis. (medicaltourismco.com)
According to a study by Castro-Manrreza and Montesinos2 published in 2015 shows that mesenchymal stem cells are known to regulate the immunity system, and improve the symptoms associated with MS by reducing the inflammation of the central nervous system and the loss of the myelin sheath (demyelination). (medicaltourismco.com)
Recently, mesenchymal stem cells (MSCs) have been suggested for use in the cell-based treatment of cartilage lesions. (researchgate.net)
NC-MSCs are mesenchymal stem cells derived from a single donor's fibroblasts that have been dedifferentiated into an induced pluripotent stem cell (iPSC) master cell bank, thereby avoiding the need to source additional donor cells. (prnewswire.com)

Pluripotent cells are implanted in a hydroxyapatite-coated poly- L -lactic acid scaffold that releases bone morphogenic protein 2. (nature.com)
The implants achieve rapid and efficient repair of a calvarial defect with a very low incidence of teratomas (they are seen in 2 of 42 mice) after injection of 1 million human pluripotent cells. (nature.com)
The discovery in 2006 that human and mouse fibroblasts could be reprogrammed to generate induced pluripotent stem cells (iPSCs) with qualities remarkably similar to embryonic stem cells has created a valuable new source of pluripotent cells for drug discovery, cell therapy, and basic research. (thermofisher.com)
Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)

Neural stem cells (NSCs) are self-renewing, multipotent cells that firstly generate the radial glial progenitor cells that generate the neurons and glia of the nervous system of all animals during embryonic development. (wikipedia.org)
Some neural progenitor stem cells persist in highly restricted regions in the adult vertebrate brain and continue to produce neurons throughout life. (wikipedia.org)
NSCs primarily differentiate into neurons, astrocytes, and oligodendrocytes. (wikipedia.org)
Neural stem cells are more specialized than ESCs because they only generate radial glial cells that give rise to the neurons and to glia of the central nervous system (CNS). (wikipedia.org)
Adult NSCs differentiate into new neurons within the adult subventricular zone (SVZ), a remnant of the embryonic germinal neuroepithelium, as well as the dentate gyrus of the hippocampus. (wikipedia.org)
These neurospheres can differentiate to form the specified neurons, glial cells, and oligodendrocytes. (wikipedia.org)
One example is a neuroblast migrating towards the olfactory bulb to differentiate into periglomercular or granule neurons which have a radial migration pattern rather than a tangential one. (wikipedia.org)
Morphological, molecular and functional analysis of the organoids pointed to characteristics of this cortical malformation such as impaired cell proliferation, neuronal network hyperexcitability, dysmorphic neurons, and the presence of balloon cells, so called because of their shape, with a neuron-like nucleus and cytoplasm similar to an astrocyte's. (eurekalert.org)
Here, the stem cells are shown expressing various markers and differentiating into neurons. (livescience.com)
Reprogrammed cells could then develop into a multitude of cell types, including the neurons found in the brain and spinal cord. (livescience.com)
However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
Stem Derived Dopamine Neurons: Will They Replace DBS as the Leading Neurosurgical Treatment for Parkinson's Disease? (lu.se)

There are two basic types of stem cell: adult stem cells, which are limited in their ability to differentiate, and embryonic stem cells (ESCs), which are pluripotent and have the capability of differentiating into any cell type. (wikipedia.org)
While the appeal of embryonic cells has been their ability to differentiate into any type of cell, there has been significant ethical, moral, and spiritual controversy surrounding their use. (prnewswire.com)

While a few studies have attempted reprogramming of glioblastoma (GBM) cells toward a neuronal fate, this approach has not yet been used to induce differentiation into other lineages and in vivo data on reduction in tumorigenicity are limited. (lu.se)

For example, hematopoietic stem cells are a type of adult stem cell found in bone marrow. (healthline.com)
Doctors have been performing stem cell transplants, also known as bone marrow transplants, for decades using hematopoietic stem cells in order to treat certain types of cancer. (healthline.com)
For example, because hematopoietic stem cells can reconstitute the entire blood system, bone marrow transplantation has long been used in the clinic to treat various diseases. (articlecity.com)
00:39 police is these are hematopoietic stem cells. (yale.edu)
Discovery of new hematopoietic tissue and discovery of multinucleated giant blood cells in D ananassae. (nottingham.ac.uk)
In early blood cells, called hematopoietic stem cells, the methylation patterns established by DNA methyltransferase 3 alpha promote maturation (differentiation) into different blood cell types. (medlineplus.gov)
Researchers speculate that the altered gene activity prevents hematopoietic stem cells from differentiating normally, which leads to the overproduction of abnormal, immature white blood cells characteristic of acute myeloid leukemia. (medlineplus.gov)
4 It also occurs in neutropenic cancer patients and hematopoietic stem cell transplant or solid organ transplant recipients. (cdc.gov)

When dissociated, they can be co-cultured with SC-like adipose-derived stem cells (ASC), providing a valuable model to study in vitro nerve regeneration and myelination, mimicking the in vivo environment at the injury site. (jove.com)

The findings also help explain why the process of differentiating stem cells into specific lineages in the laboratory has been highly inefficient. (sciencedaily.com)
Pushing mature cells into other lineages may offer a new way to replace cells involved in blood diseases and neurodegenerative disorders such as Alzheimer s disease. (innovations-report.com)
At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)

The fact that the DNA of a fully differentiated (adult) cell could be stimulated to revert to a condition comparable to that of a newly fertilized egg and to repeat the process of embryonic development demonstrates that all the genes in differentiated cells retain their functional capacity, although only a few are active. (who.int)
Aerosolized nano-zinc oxide (nZnO) exposure alters the cellular composition of the fully differentiated human airway epithelium. (cdc.gov)
Fully differentiated cells were exposed to aerosolized nZnO for 6 minutes, at the indicated doses, using the VitroCell® Cloud Alpha 12 system. (cdc.gov)
The presence of all four major cell types at protein level was confirmed by IF staining of the fully differentiated airway epithelium. (cdc.gov)

It appears there is at least a small window where terminally differentiated cells vacillate on which identity to adopt. (innovations-report.com)
Results: qPCR analysis of the differentiation time-course shows a steady increase in markers of terminally differentiated cells, such as club (SCGB1A1), goblet (MUC5AC) and ciliated cells (FoxJ1). (cdc.gov)

Adult Stem Cells: The New Plastic Surgery? (scitizen.com)
27 Jun, 2008 05:23 pm A new article in Live Science predicts that adult stem cells may replace silicone breast implants and other contemporary plastic surgery techniques. (scitizen.com)
Together with other published results, these observations blur the concept of fixed cell types and provide new insights concerning potential uses for adult stem cells. (innovations-report.com)
Adult stem cells have a misleading name, because they are also found in infants and children. (healthline.com)
Adult stem cells can't differentiate into as many other types of cells as embryonic stem cells can. (healthline.com)
Scientists have recently discovered how to turn adult stem cells into pluripotent stem cells. (healthline.com)
To create iPSCs, scientists genetically reprogram the adult stem cells so they behave like embryonic stem cells. (healthline.com)
Adult stem cells don't present any ethical problems. (healthline.com)
Adult stem cells, which are present in small amounts in adult tissue but less adaptable than embryonic stem cells, making their use in medical treatments more challenging. (articlecity.com)
Traditionally, scientists have worked with both embryonic and adult stem cells for research tools, as well as for cellular therapy. (prnewswire.com)
Although some adult stem cells do have differentiation capacity, it is often limited in nature, which results in fewer options for use. (prnewswire.com)

We take advantage of the similarity between neural developmental processes and cancer hierarchy to mitigate, if not completely abolish, the malignant nature of tumor cells and pave the way for new intervention strategies. (lu.se)
However, the smaller the residual mass, the lower the chance that it harbors viable tumor cells. (medscape.com)

Epidermal growth factor (EGF) and fibroblast growth factor (FGF) are mitogens that promote neural progenitor and stem cell growth in vitro, though other factors synthesized by the neural progenitor and stem cell populations are also required for optimal growth. (wikipedia.org)
The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo. (scielo.br)
The organoids were cultured in vitro to simulate the morphology and functioning of part of the brain from skin cells donated by four patients with severe epilepsy treated at UNICAMP's teaching hospital (Hospital de Clínicas). (eurekalert.org)
Skoglund G, Lainé J, Darabi R , Fournier E, Perlingeiro R, Tabti N. Physiological and ultrastructural features of human induced pluripotent and embryonic stem cell-derived skeletal myocytes in vitro. (uh.edu)
02:47 and red blood cells in vitro. (yale.edu)
Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)

Sites of loosely packed extracellular matrix (ECM) also offer stem and progenitor cell optimal space to proliferate and differentiate. (europa.eu)
The basal cell (stem/progenitor cell) marker (KRT5) shows an initial increase in expression, but did not vary much during differentiation. (cdc.gov)

Processes of differentiated central glia-4 oligodendrocytes were insensitive to lysophosphatidic acid treatment but cell bodies became phase dark, indicating cell spreading on the poly-l-lysine substratum. (nih.gov)
The results show that lysophosphatidic acid signalling pathways influence retraction of processes in oligodendrocyte precursors but that this effect changes as oligodendrocytes differentiate. (nih.gov)

In type 2 diabetes, the most common form, tissues in the body lose their sensitivity to insulin, and pancreatic beta cells can't make enough insulin to keep glucose levels in check. (pharmaceuticalintelligence.com)
In the early stage of skin trauma, the basal cells at the edge of the wound begin to proliferate and form a single layer of epithelium, which covers the surface of the granulation tissues of the wound and then differentiate into squamous epithelium [ 1 , 2 ]. (biomedcentral.com)
More importantly, miR-214 is implicated in self-renewal of skin tissues and found to be significantly downregulated in proliferation and differentiation of HFSc into transit-amplifying cells [ 18 ]. (biomedcentral.com)
This special type of stem cell is present in many tissues and restores bone, connective tissues and fat. (europa.eu)
27 Jun, 2007 06:08 pm Stem cells have the potential to become all the cells and tissues in the human body. (scitizen.com)
These stem cells come from developed organs and tissues in the body. (healthline.com)
Although different stem cells, morphogens and scaffolds can be combined to simulate natural odontogenic regeneration, it is crucial to determine which options most closely replicate the characteristics of human dental tissues. (scielo.br)
Similarly, the transplantation of other tissue-specific stem cells, such as stem cells isolated from epithelial and neural tissues, can treat mouse disease models and human patients in which epithelial and neural cells are damaged. (articlecity.com)
The possibilities arising from these characteristics have resulted in great commercial interest, with potential applications ranging from the use of stem cells in reversal and treatment of disease, to targeted cell therapy, tissue regeneration, pharmacological testing on cell-specific tissues, and more. (prnewswire.com)
Studying how induced pluripotent stem cells develop into various tissues could also shed light on disorders that are due to malfunctions in development. (livescience.com)
Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)

Differences in the size of the central nervous system are among the most important distinctions between the species and thus mutations in the genes that regulate the size of the neural stem cell compartment are among the most important drivers of vertebrate evolution. (wikipedia.org)
Instead, it supports the idea that cells differentiate through the collective behavior of multiple genes in a network that ultimately leads to just a few endpoints -- just as a marble on a hilltop can travel a nearly infinite number of downward paths, only to arrive in the same valley. (sciencedaily.com)
Again, they found tremendous variability within the apparently uniform cell population: more than 3,900 genes were differentially expressed (turned "on" or "off") between the low- and high-Sca-1 cells. (sciencedaily.com)
Thus, we know that the simple cells of an egg are preformed in the sense that they contain a preformed instruction set for development which is encoded in their genes. (encyclopedia.com)
These include generation of knock-in reporter stem cell lines to study myogenic differentiation, CRISPR/Cas9 gene targeting methods to correct mutated genes in muscular dystrophies, and using bioscaffolds, seeded with stem cells, to treat skeletal muscle injuries. (uh.edu)
Found within Wharton's jelly - which is easily harvested from what would otherwise be post-natal medical waste - are several distinct stem cell genes. (articlecity.com)
The model also predicts that reprogramming the network from a differentiated state, in particular the endoderm state, into a stem cell state, is best achieved by over-expressing Nanog, rather than by suppression of differentiation genes such as Gata-6. (lu.se)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)

In this study, we investigate the expression of differentiated Schwann cell markers by exposing of endometrial stem cells with induction media including FGF2/FSK/HRG/RA. (scirp.org)
Using immunocytochemistry, we show that differentiated cells express S100 and P75 markers. (scirp.org)
While scientists have been able to change stem cells into insulin-producing cells, these cells don't have markers that indicate they are beta cells, and they aren't responsive to glucose. (pharmaceuticalintelligence.com)
The cultured beta cells had specific markers that were found on normal beta cells which displayed changes in calcium levels when exposed to glucose and packaged insulin into granules. (pharmaceuticalintelligence.com)
Several markers for AF phenotype (aggrecan, collagen type I and glypican-3) were expressed in both hAFCs and differentiated hASCs. (utwente.nl)
Further, when those MSCs were analyzed for their specific marker expression using flowcytometry, they were found to be positive for CD29, CD73, CD90, and CD105 and negative for CD34 and CD45 stem cell markers respectively. (journalcra.com)
Differentiated cells express typical glial markers and show a marked decrease in their proliferative state. (lu.se)
Optimal differentiation was verified by qPCR and immunofluorescent (IF) staining of cell-type specific markers. (cdc.gov)
Cells were harvested at 24 hours or 7 days post exposure, to investigate changes in (a) inflammation, (b) oxidative stress-related, and (c) cell-type specific markers using qPCR and/or IF staining. (cdc.gov)
Exposure to high dose of nZnO induced a significant decrease in expression of club (SCGB1A1), goblet (MUC5AC) and ciliated cells (FoxJ1) markers at mRNA levels. (cdc.gov)

In vivo engraftment of stem cell-derived cells into damaged or diseased tissue has many challenges. (nature.com)
For one, if pluripotent stem cells are introduced in numbers large enough for tissue regeneration, there is a high risk of teratomas in an immunodeficient mouse (a standard research model for studying engraftment). (nature.com)
Using this system, called the Nodal/Activin pathway, scientists will be able to control the eventual type of tissue the stem cells will develop into, simply by adjusting the cell's chemical environment. (rejuvalife.md)
Dr. Kian Leong Lee, who led the study, says this finding "paves the way for advanced studies in cell regeneration and tissue repair, which could ultimately lead to its use in personalized medicine, where stem cells from the same patient could be manipulated to make other types of cells that are genetically matched to the donor. (rejuvalife.md)
Critical to the hypothesis is the experimental fact that human endometrial-derived stem cells (hEnSCs) as multipotent accessible source of cells are known as useful cell candidates in the field of nerve tissue engineering. (scirp.org)
These novel differentiated cells in fibrin gel might open new opportunities for the management of cell survival and neurotrophic potential in tissue engineering approaches for nerve repair. (scirp.org)
Quiescent stem cells are Type B that are able to remain in the quiescent state due to the renewable tissue provided by the specific niches composed of blood vessels, astrocytes, microglia, ependymal cells, and extracellular matrix present within the brain. (wikipedia.org)
Stem cells are essential for regeneration and repair of worn-out or injured tissue. (europa.eu)
There, MSC differentiate and regenerate tissue integrity. (europa.eu)
The Fragility of Cryopreserved Insulin-producing Cells Differentiated from Adipose-tissue-derived Stem Cells. (bvsalud.org)
The aim of our study is to determine whether insulin -producing cells (IPCs) differentiated from adipose- tissue -derived stem cells (ADSCs) can be cryopreserved. (bvsalud.org)
8 Jun, 2007 04:13 pm Stem cells provide the starting material for the development and repair of every organ and tissue in the body and they are present in all stages of life. (scitizen.com)
This means they can potentially produce new cells for any organ or tissue. (healthline.com)
Hard tissue formation in a porous HA/TCP ceramic scaffold loaded with stromal cells derived from dental pulp and bone marrow. (scielo.br)
With this raw material, biomedical firms can create stem cell lines that, among other things, aid recuperation via the regeneration of tissue that has been lost or damaged. (articlecity.com)
Current research is focused on growing a wide range of new tissue from stem cells, including muscle, blood, brain, and cartilage cells. (articlecity.com)
They have many of the positive characteristics of embryonic stem cells while sourcing material is far less challenging, since postpartum tissue can be used. (articlecity.com)
In other words, dead people can yield living cells that can be converted into any cell or tissue in the body. (livescience.com)
Mature cells can be made or induced to become immature cells, known as pluripotent stem cells , which have the ability to become any tissue in the body and potentially can replace cells destroyed by disease or injury. (livescience.com)
Fibroblasts are the most common cells of connective tissue in animals, and they synthesize the extracellular matrix, the complex scaffolding between cells. (livescience.com)
Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)

Scientists believed that stem cell differentiation was controlled by multiple, highly specific and complex chemical systems, each capable of producing only a limited variety of cells. (rejuvalife.md)
Scientists have found a way to possibly avoid using embryonic stem. (scitizen.com)
Scientists at the University of Pennsylvania School of Veterinary Medicine have found a new wrinkle in the developmental biology dogma that cell differentiation occurs irreversibly as stem cells give rise to increasingly specialized types of offspring cells. (innovations-report.com)
Scientists also say this approach offers the potential for converting lymphoma cells that are resistant to treatment into more manageable forms of cancer. (innovations-report.com)
Since stem cells have the ability to turn into various other types of cells, scientists believe that they can be useful for treating and understanding diseases. (healthline.com)
Scientists are hoping that the cells can be made from someone's own skin to treat a disease. (healthline.com)
Now scientists have harvested such cells from the scalps and brain linings of human corpses and reprogrammed them into stem cells. (livescience.com)
Now scientists have taken fibroblasts from the scalps and the brain linings of 146 human brain donors and grown induced pluripotent stem cells from them as well. (livescience.com)
Bringing together Universities and high school students, UniStem Day is an opportunity to foster learning, discovery and debate in the field of stem cell research - inspiring the scientists of tomorrow. (lu.se)
These and many more questions will be answered by PhD students, postdoctoral fellows, and senior scientists from Lund Stem Cell Center during UniStem Day 2024. (lu.se)

Assessment of the myogenic stem cell compartment following transplantation of pax3/pax7-induced embryonic stem cell-derived progenitors. (uh.edu)

2 Despite having high metabolic activity, human CECs (hCECs) do not proliferate in vivo because these cells arrest in the G1 phase of the cell cycle. (lww.com)
Surprisingly, scalp cells did proliferate more and grew more rapidly than dura mater cells. (livescience.com)

Schwann cells (SCs) have important roles in development, myelination and regeneration in the peripheral nervous system. (scirp.org)
The dynamic epithelial-mesenchymal crosstalk characteristic of hair follicle stem cells (HFSc) from embryonic development to adulthood can be found during normal hair follicle growth and cyclic regeneration [ 7 ]. (biomedcentral.com)
Dr Anand Srivastava: How stem cells help in regeneration? (medicaltourismco.com)
The main interest of Dr. Darabi's lab is to use pluripotent stem cells for skeletal muscle regeneration in muscular dystrophies and muscle injuries. (uh.edu)

These new types of cells are called induced pluripotent stem cells (iPSCs). (healthline.com)

These group 2 or type 2 innate lymphoid cells (ILC2 cells) represent a critical source of type 2 cytokines in vivo and serve an important role in orchestrating the type 2 response to helminths and allergens. (nature.com)
This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. (scielo.br)
Most importantly, forced differentiation substantially reduces tumorigenicity of GBM cells in an in vivo xenotransplantation model. (lu.se)

Further characterization of ILC2 cell biology will enhance the understanding of type 2 responses and may identify new treatments for asthma, allergies and parasitic infections. (nature.com)
Led by Sui Huang, MD, PhD, a Visiting Associate Professor in the Children's Hospital Boston Vascular Biology Program (now also on the faculty of the University of Calgary), and Hannah Chang, an MD/PhD student in Children's Vascular Biology Program, the researchers examined how blood stem cells "decide" to become white blood cell progenitors or red blood cell progenitors. (sciencedaily.com)
At the core of the network reside Oct4, Sox2 and Nanog, into embryonic stem cells [1,2,3,4,5], have made major inroads which form a self-organized core of the TFs maintaining into stem cell biology. (lu.se)
However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)

Our complete portfolio of stem cell research products and services provides optimized tools for each step of the stem cell workflow: from reprogramming somatic cells to iPSC expansion, validation, and differentiation. (thermofisher.com)
Reprogramming of somatic cells for iPSC generation can be accomplished with a number of technologies. (thermofisher.com)
Analyze stem cells and their derivatives using a wide range of cellular and molecular characterization methods, as validation is critical in iPSC research. (thermofisher.com)
Induced pluripotent stem cells represent one of the most promising research advances within the past decade, making this a valuable report for both executives and investors to use to optimally position themselves to sell iPSC products. (prnewswire.com)
Chad and I have both been principal investigators on the induced pluripotent stem cell (IPSC) grant. (medscape.com)

Apart from this, it was observed that those MSCs, when induced to differentiate into cardiomyocytes, they successfully differentiated into cardiomyocyte like cells with flat, wide, myotube like structure with sarcomeric organization, which were later confirmed to be cardiomyocytes by qRT-PCR. (journalcra.com)
Thus, this study shows that MSCs were isolated from human bone-marrow and subsequently differentiated into cardiomyocytes with 100% success rate. (journalcra.com)
6- Differentiation of iPSCs into cardiomyocytes and endothelial cells for disease modeling and screening. (uh.edu)
Highly functional cardiomyocytes-the muscle cells responsible for the contraction of the heart-are important for disease modeling, drug screening, and other regenerative medicine approaches. (universityresearchpark.org)
the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)

Blood stem cells with low levels of Sca-1 differentiated into red blood cell progenitors seven times more often than cells high in Sca-1 when exposed to erythropoietin, a growth factor that promotes red blood cell production. (sciencedaily.com)
Wu J, Matthias N, Lo J, Ortiz-Vitali JL, Shieh AW, Wang SH, Darabi R . A Myogenic Double-Reporter Human Pluripotent Stem Cell Line Allows Prospective Isolation of Skeletal Muscle Progenitors. (uh.edu)
02:14 progenitors that make red blood cells. (yale.edu)

Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells. (scielo.br)

In the adult mammalian brain, the subgranular zone in the hippocampal dentate gyrus, the subventricular zone around the lateral ventricles, and the hypothalamus (precisely in the dorsal α1, α2 region and the "hypothalamic proliferative region", located in the adjacent median eminence) have been reported to contain neural stem cells. (wikipedia.org)
Some neural cells are migrated from the SVZ along the rostral migratory stream which contains a marrow-like structure with ependymal cells and astrocytes when stimulated. (wikipedia.org)
The neuroblasts form tight chains and migrate towards the specified site of cell damage to repair or replace neural cells. (wikipedia.org)
Neural stem cell proliferation declines as a consequence of aging. (wikipedia.org)
Because FOX proteins regulate neural stem cell homeostasis, FOX proteins have been used to protect neural stem cells by inhibiting Wnt signaling. (wikipedia.org)
Therapeutic Effects of Conditioned Medium of Neural Differentiated Human Bone Marrow-Derived Stem Cells on Rotenone-Induced Alpha-Synuclein Aggregation and Apoptosis. (logosbio.com.cn)
The cells were reprogrammed to become pluripotent stem cells and differentiate into neural cells. (eurekalert.org)
This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)

Artificial reproduction of Descemet membrane with respect to topography and similar stiffness offers a potential innovative way to bioengineer a functional CEC monolayer from autologous stem cells. (lww.com)
The researchers tested more than 150 combinations of over 70 compounds to figure out a method to produce functional human beta cells from the cultured stem cells which when added in exact combinations over a period of several weeks, they transformed human pluripotent stem cells into beta cells that functioned similarly to normal adult beta cells. (pharmaceuticalintelligence.com)
While this observation is interesting and provides useful information to understand the functional correlates of stem cell differentiation, further studies are required to clarify the molecular and mechanistic correlates of these changes. (agu.edu.tr)
Released PTH increased osteocalcin expression and alizarin red S staining in preosteoblastic cells, indicating its functional activity. (ewha.ac.kr)
Production of a functional cell wall-anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824. (nottingham.ac.uk)

Stem Cells International 2021:6658271. (logosbio.com.cn)

The other approach is to develop functioning beta cells from stem cells which have the potential to transform into many different cell types. (pharmaceuticalintelligence.com)
Stem cells are characterized by their capacity to differentiate into multiple cell types. (wikipedia.org)
Adipose-derived mesenchymal stromal cells (ASCs) are multipotent stem cells which can differentiate into various cell types, including osteocytes and adipocytes. (agu.edu.tr)
The researchers have shown that certain mouse cells retain an ability to oscillate between very distinct blood cell types - B-cells and macrophages - long after what has been commonly regarded as the point of no return. (innovations-report.com)
Until recently, it was believed that the various cell types of the blood are generated from stem cells in a controlled but irreversible fashion," said James Hagman, an immunology researcher at the National Jewish Medical and Research Center in Denver who was not involved in this research. (innovations-report.com)
There are several types of stem cells that can be used for different purposes. (healthline.com)
They make new red blood cells, white blood cells, and other types of blood cells. (healthline.com)
They can differentiate into all types of specialized cells in the body. (healthline.com)
Some study the cell growth, or how specialized cell types differentiate from stem cells. (encyclopedia.com)
In addition, their "unspecialized" nature allows them to differentiate into a wide variety of specialized cell types. (prnewswire.com)
Mechanistic insight into heterogeneity of trans-plasma membrane electron transport in cancer cell types. (nottingham.ac.uk)
Direct cellular reprogramming has recently gained attention of cancer researchers for the possibility to convert undifferentiated cancer cells into more differentiated, postmitotic cell types. (lu.se)
The enzyme also functions in early cells that can give rise to more mature cell types. (medlineplus.gov)
The adult human airway epithelium is comprised of four major cell types, basal, club, goblet, and ciliated cells. (cdc.gov)
Any change in the numbers of these cell types is termed as 'airway remodeling', which is a disease-related phenotype in several lung diseases. (cdc.gov)
Of the three main types of testicular cancer, nonseminomatous germ cell tumors (NSGCTs) are second only to seminomas in terms of frequency. (medscape.com)
The developmental history of specific differentiated cell types as traced back to the original STEM CELLS in the embryo. (bvsalud.org)

It s possible that by targeting this gene with drugs, we may be able to convert malignant B-cell lymphomas into much less harmful histiocytomas, tumors composed of relatively inactive macrophages. (innovations-report.com)
Extragonadal germinal cell tumors (EGGCTs) are rare tumors that predominantly affect young males. (medscape.com)
The only known risk factor for extragonadal germ cell tumors is Klinefelter syndrome (47XXY), which is associated with mediastinal nonseminomatous germ cell tumors, which are characterized by their location on the midline from the pineal gland to the coccyx. (medscape.com)
[ 1 , 2 ] In extragonadal germ cell tumors, no evidence of a primary malignancy is present in either the testes or ovaries by radiologic imaging or physical examination. (medscape.com)
Controversy remains regarding the origin of extragonadal germ cell tumors. (medscape.com)
The classic theory suggests that germ cell tumors (GCTs) in these areas are derived from local transformation of primordial germ cells misplaced during embryogenesis. (medscape.com)
According to this theory, the differences in phenotypes expressed by mediastinal germ cell tumors (MGCTs) and gonadal germ cell tumors may be explained by differences in the cellular environment between the gonad and the anterior mediastinum. (medscape.com)
Some retroperitoneal extragonadal germ cell tumors may represent metastases from a testicular cancer , with subsequent spontaneous necrosis of the primary tumor. (medscape.com)
Such tumors may arise in males of nearly any age and may be of germ cell or non-germ cell origin. (medscape.com)
This article addresses the demographics, histology, prognosis, and treatment of nonseminomatous germ cell tumors (NSGCTs). (medscape.com)
germ cell tumors , non-germ cell tumors, and extragonadal tumors . (medscape.com)
Germ cell tumors, which are the most common, are classified as either seminoma or nonseminoma, based on histology. (medscape.com)
Literature suggests that the only known risk factor for extragonadal germ cell tumors (EGCTs) is Klinefelter syndrome (47XXY), which is associated with mediastinal nonseminomatous germ cell tumors. (medscape.com)
Extragonadal germ cell tumors produce a rich symptomatology and may reach large volumes if they arise in silent areas. (medscape.com)
Hematologic malignancies are frequently associated with mediastinal germ cell tumors. (medscape.com)
Highly differentiated yolk-sac tumors make up 30% of mediastinal germ cell tumors, providing a possible basis for this association. (medscape.com)
The balance of the p53-mdm2 interaction has been shown to be disrupted in intracranial germ cell tumors (ICGCTs). (medscape.com)

The findings, published in the May 22 issue of Nature, give a glimpse into how that collective behavior works, and show that cell populations maintain a built-in variability that nature can harness for change under the right conditions. (sciencedaily.com)
They began by examining populations of seemingly identical blood stem cells, and found that a cell marker of "stemness," a protein called Sca-1, was actually present in highly variable amounts from cell to cell -- in fact, they found a 1,000-fold range. (sciencedaily.com)
Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)

abstract = "Parathyroid cells release parathyroid hormone (PTH), which controls calcium homeostasis. (ewha.ac.kr)

During the process of harvesting embryotic stem cells, the embryo is destroyed. (healthline.com)

However, the worldwide shortages of corneal donor material generate a strong demand for personalized stem cell-based alternative therapies. (lww.com)
A new human model developed by researchers at the University of Campinas (UNICAMP) in the state of São Paulo, Brazil, using organoids cultured from patients' cells has now opened up opportunities to test more specific therapies and medications. (eurekalert.org)
As such, this work could help lead to novel stem cell therapies and shed light on a variety of mental disorders, such as schizophrenia , autism and bipolar disorder, which may stem from problems with development, researchers say. (livescience.com)
Cells from corpses might play a key role in developing future stem cell therapies . (livescience.com)

In this experiment, the researchers grew a human embryonic stem cell line and 2 human-induced pluripotent stem cell lines in a culture system that allowed them to produce large numbers of cells. (pharmaceuticalintelligence.com)

During the embryonic development of vertebrates, NSCs transition into radial glial cells (RGCs) also known as radial glial progenitor cells, (RGPs) and reside in a transient zone called the ventricular zone (VZ). (wikipedia.org)

Lund Stem Cell Center will host UniStem Day 2024 on Friday, 22 March, 2024, with high school students from all over Skåne. (lu.se)
Johan Jakobsson, a professor at Lund University and research group leader at MultiPark and Lund Stem Cell Center, explains, "LINE-1 retrotransposons are a rich source of genetic sequences that we suspect have shaped the evolution of the human brain, and we now have the tools to explore their role in brain development. (lu.se)

In the bone marrow MSC share the niche with haematopoietic stem cells (HSC). (europa.eu)
The key attributes like isolation efficiency, cell yield, colony-forming unit-fibroblast (CFU-F) frequency, phenotypic characteristics, and multi-lineage differentiation capacity were determined for the isolated MSCs from bone marrow specimens. (journalcra.com)
It was observed that the bone marrow possessed a good number of nucleated cells and had a high yield of adherent cells. (journalcra.com)

During the last several years, his lab has developed novel methods for multi-lineage differentiation of pluripotent stem cells for cell therapy in mice models of muscular dystrophies and injuries. (uh.edu)
Methods: Normal human bronchial epithelial cells (NHBEpC) (at ≥ 90% viability) were seeded on 12-well porous 0.4 µm- cell culture inserts in basal cell culture medium. (cdc.gov)

The cells, called NoveCite Cells or NC-MSCs, are made by Novellus, Inc. ("Novellus"), a Cambridge-based biotechnology company, using its patented mRNA-based cell-reprogramming process. (prnewswire.com)

In other words, unfaithful cells that have strayed from their original identity can come to their senses and return to the original lineage," Thomas-Tikhonenko said. (innovations-report.com)
Citation: Chickarmane V, Peterson C (2008) A Computational Model for Understanding Stem Cell, Trophectoderm and Endoderm Lineage Determination. (lu.se)

Nov. 22, 2023 Tandem solar cells based on perovskite semiconductors convert sunlight to electricity more efficiently than conventional silicon solar cells. (sciencedaily.com)
2023). Comparing the adult and prepubertal testis: Metabolic Transitions and the Change in the Spermatogonial Stem Cell Metabolic Microenvironment. (cdc.gov)

These cells have been described as embryonic stem cells (ESCs) and. (scitizen.com)

In this study, we used 2-photon live Ca2+ imaging to assess the fraction of cells showing spontaneous oscillations and the frequency of the oscillation (measured as interpeak interval-IPI) in ASCs undergoing osteogenic or adipogenic differentiation, using undifferentiated ASCs as controls. (agu.edu.tr)
Both adipogenic and osteogenic differentiation resulted in a reduction in Ca2+ dynamics, such as the fraction of cells showing intracellular Ca2+ oscillations and their frequency. (agu.edu.tr)
Adipogenic differentiation was associated with a more pronounced reduction of Ca2+ dynamics compared to cells differentiating towards the osteogenic fate. (agu.edu.tr)
These data suggest that adipogenic and osteogenic differentiation cell fates are associated with specific changes in spontaneous Ca2+ dynamics over time. (agu.edu.tr)

Our results now suggest that 4-MU treatment of MSC not only affects the formation of SG via increased levels of O-linked glycosylation of cellular proteins but also that MSC cultivated under osteogenic stimulation develop SG, which in turn support MSC differentiation into osteogenic precursor cells. (europa.eu)

The intricate molecular mechanisms involved in the regenerative process of the normal intestine and the identity of putative somatic intestinal stem cells have become clear. (mdpi.com)
Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)
The nucleus of an adult somatic cell (such as a skin cell) is removed and transferred to an enucleated egg, which is then stimulated with electric current or chemicals to activate cell division. (who.int)
if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
Somatic DNMT3A gene mutations are also found relatively frequently in another form of blood cancer called T-cell acute lymphoblastic leukemia. (medlineplus.gov)

NSCs are stimulated to begin differentiation via exogenous cues from the microenvironment, or stem cell niche. (wikipedia.org)
Cell viability assay Cell viability of NSCs was evaluated by making use of the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. (forgetmenotinitiative.org)

Our differentiation portfolio allows optimal growth, expansion and storage of differentiated cells-enabling faster, more efficient systems. (thermofisher.com)

Transplanted human pancreatic cells from deceased donors have been successfully used to treat people with type 1 diabetes. (pharmaceuticalintelligence.com)
These cells have been successfully used to treat children with blood cancers, such as leukemia, and certain genetic blood disorders. (healthline.com)
However, bacteria and fungi on the skin can wreak havoc on the culturing processes used to grow cells in labs, making the process tricky to successfully carry out. (livescience.com)
Successfully reprogramming induced pluripotent stem cells so they behave like the cells they are meant to replace means that samples of the mimicked cells must be present for comparison. (livescience.com)

Dental pulp stem cells (DPSC) seem to be the most appropriate cell group for the regenerative approach. (scielo.br)
Such an approach is highly relevant to regenerative medicine since it allows for a rapid search over the host of possibilities for reprogramming to a stem cell state. (lu.se)
They may also serve as an unlimited source of therapeutically active cells for regenerative medi- cine. (lu.se)
UniStem Day is the largest educational outreach initiative on stem cells and regenerative medicine in Europe. (lu.se)

1 If the number of corneal endothelial cells (CECs) falls below a certain threshold because of traumatic injury, disease, or normal aging processes, functionality of the CE is decreased, and the cornea swells and becomes milky, leading to eventual vision loss. (lww.com)
A major component outside of the cells, which notably is distinctly involved in controlling and guiding many cellular processes, is the polysaccharide hyaluronan (HA). (europa.eu)
Treated cells undergo a remarkable shift in structure, acquiring an astrocyte-like morphology with star-shaped bodies and radial branched processes. (lu.se)

Moreover, DNA methyltransferase 1 (DNMT1) deficiency was involved in the differentiation of hair follicle stem cell (HFSc), but the molecular mechanisms remain unknown. (biomedcentral.com)

We decided to use the three-dimensional culture of Schwann cells differentiated from endometrial stem cell in fibrin gel. (scirp.org)
Matthias N, Hunt SD, Wu J, Lo J, Smith Callahan LA, Li Y, Huard J, Darabi R . Volumetric muscle loss injury repair using in situ fibrin gel cast seeded with muscle-derived stem cells (MDSCs). (uh.edu)

The initiation of type 2 immune responses by the epithelial cell-derived cytokines IL-25, IL-33 and TSLP has been an area of extensive research in the past decade. (nature.com)
Saenz, S.A., Taylor, B.C. & Artis, D. Welcome to the neighborhood: epithelial cell-derived cytokines license innate and adaptive immune responses at mucosal sites. (nature.com)
Differentiation of epithelial cells is the key process of epidermal wound repair. (biomedcentral.com)
Thus, the aim of this study was to investigate if aerosolized nZnO exposure leads to changes in the cellular composition of the airway epithelium, using air-liquid interface (ALI)-differentiated primary human bronchial epithelial cells. (cdc.gov)

Stem Cell Therapy for Multiple Sclerosis in Mexico can help which is safe and effective. (medicaltourismco.com)
We at Medical Tourism Corporation have collaborated with the global leader in stem cell research and therapy, for treating this chronic disease that attacks the central nervous system. (medicaltourismco.com)
However, the stem cell therapy cost in Mexico for MS is nearly half as compared to its counterparts in the USA. (medicaltourismco.com)
Therefore, stem cell therapy for multiple sclerosis in Mexico can prove effective as it will not only stop further nerve cell degeneration but also promote regrowth of the damaged cells. (medicaltourismco.com)
We offer choices in integration-free reprogramming technologies and services that fit your research and translational needs including our Cell Therapy Systems (CTS) Products, which are designed for clinical and translational research applications. (thermofisher.com)
From feeder-based and feeder-free media to optimized passaging reagents and substrates to media for stem cell therapy research, Gibco products enable you to culture with confidence. (thermofisher.com)
Dental pulp stem cells: state of the art and suggestions for a true translation of research into therapy. (scielo.br)
However, the standardization of the manufacturing process of MSC-based cell therapy medicinal products in compliance with the requirements of the local authorities is obligatory and will allow us to obtain the necessary permits for product administration according to its intended use. (mdpi.com)
Towards cell replacement therapy in Parkinson's disease. (lu.se)

Chinese researchers believe they've gotten a step closer to producing all-weather photovoltaics by integrating phosphors into solar cells. (materialstoday.com)
18 Sep, 2007 12:13 pm Rice University researchers have engineered musculoskeletal cartilages with human embryonic stem cells, with the hope of eventually using the neotissue. (scitizen.com)
6 Jun, 2007 07:00 pm Researchers have reprogrammed skin cells into embryonic stem cells. (scitizen.com)
Since the initial discovery of the self-renewing properties of stem cells in the early 1960s - pioneered by a small team of researchers at the Ontario Cancer Institute [3] - the understanding of the power and diversity of stem cells has increased rapidly. (articlecity.com)
A distinctive feature of this report is an end-user survey of 273 researchers (131 U.S. / 143 International) that identify as having induced pluripotent stem cells as a research focus. (prnewswire.com)
Researchers produced pluripotent stem cells from the fibroblast cells in the brain lining of human corpses. (livescience.com)

Yet, in both experiments, all three groups of cells retained characteristics of stem cells. (sciencedaily.com)
Thus, induced pluripotent stem cells represent a promising combination of adult and embryonic stem cell characteristics. (prnewswire.com)

show that teratoma formation can be minimized by transplanting the cells into an engineered niche that promotes efficient differentiation. (nature.com)
MSC reside in a quiescent state at a special site referred to as the stem cell niche. (europa.eu)
Stem cell niche. (bvsalud.org)

Previous studies have shown that human adipose stem cells (hASCs) are able to differentiate towards AF phenotype when treated with suitable growth factors. (utwente.nl)
qRT-PCR confirmed the similarity of the differentiated hASCs with AF phenotype. (utwente.nl)
This is the first study that demonstrated that hASCs can be differentiated towards AF phenotype using serum free chondrogenic medium without growth factors. (utwente.nl)

Anatomy57

Organisms16

Diseases12

Chemicals and Drugs67

Analytical, Diagnostic and Therapeutic Techniques and Equipment53

Phenomena and Processes51

Disciplines and Occupations4

Technology, Industry, Agriculture2

Information Science2

Named Groups1

Health Care1

Mesenchymal stem11

Pluripotent cells4

Neurons13

Ability to differentiate2

Neuronal1

Hematopoietic8

Adipose-derived stem cells1

Lineages3

Fully differentiated4

Terminally differentiated cells2

Adult stem11

Tumor Cells2

Vitro7

Progenitor cell2

Oligodendrocytes2

Tissues12

Genes8

Markers10

Tissue23

Scientists10

Transplantation1

Proliferate2

Regeneration4

Called induced1

Vivo3

Biology4

IPSc5

Cardiomyocytes5

Progenitors3

Stromal stem cells1

Neural9

Functional5

20211

Types17

Tumors17

Populations4

Abstract1

Embryo1

Therapies4

Human induced1

Embryonic development1

Lund Stem Cell Center2

Bone marrow3

Methods2

MSCs1

Lineage2

20232

ESCs1

Adipogenic4

Precursor cells1

Somatic5

NSCs2

Optimal1

Successfully4

Regenerative4

Processes3

Molecular1

Fibrin2

Epithelial4

Therapy9

Researchers6

Characteristics2

Niche3

Phenotype3