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  • endothelial
  • The adherence of Mø to endothelial cells is one of the first events detected in experimental animals fed an atherogenic diet. (ahajournals.org)
  • transforming growth factor-beta3 (TGFbeta3, 10-100 ng/ml), insulin-like growth factor-1 (IGF-1, 2-20 ng/ml), Ciliary NeuroTrophic Factor (CNTF, 1-10 ng/ml), or vascular endothelial cell growth factor (VEGF, 10-100 ng/ml) during differentiation under a serum free condition in Lab-Teck II chamber slides (Nalge Nunc). (arvojournals.org)
  • Cardiomyocytes and endothelial cells in the heart are in close proximity and in constant dialogue. (biologists.org)
  • The endothelial cells expressed cardiac markers also present in primary cardiac microvasculature suggesting cardiac endothelium identity. (biologists.org)
  • Glial Cells
  • We found opsin-positive and GFAP-positive cells in TGFbeta3-, IGF-1-, and CNTF-treated HNSCs, indicating the presence of photoreceptor and glial cells, where as VEGF-treated HNSCs did not show opsin immunoreactivity. (arvojournals.org)
  • tumors
  • Their new paper in the Proceedings of the National Academies of Sciences backed by experiments at Duke shows how and why some cells differentiate within tumors and how they spread. (news-medical.net)
  • Non-NK cell-treated control animals did receive the IL-2/IL-15 cytokine regimen and typically developed large tumors as indicated by luciferase expression. (nih.gov)
  • More recently, it has been suggested that therapy resistance and subsequent tumor recurrence could be mediated by the "cancer stem cell " fraction of colorectal tumors. (mcponline.org)
  • pathway
  • At the center, they found JAG1, a ligand, or small protein, that interacts with a signaling pathway critical to regulating the fate of cells. (news-medical.net)
  • In those studies, they discovered how ligands that activate the pathway go rogue to hold migrating cancer cells together and help them attach to connective tissues at new locations. (news-medical.net)
  • The notch-delta pathway creates communication between cells, but things become problematic when the receptor is jagged instead of delta,' he said. (news-medical.net)
  • In this report, we characterize several macrophage-associated proteins and functions considered relevant to atherosclerosis to determine if they are expressed by ES cells that have been differentiated down the Mø pathway. (ahajournals.org)
  • A central role for the ERK-signaling pathway in controlling Schwann cell plasticity and peripheral nerve regeneration in vivo," Neuron , vol. 73, no. 4, pp. 729-742, 2012. (hindawi.com)
  • Pathway analysis showed that "cell death " regulation is strikingly different between the two cell types. (mcponline.org)
  • genes
  • This study provides a direct comparison of transcriptional programs between pure hPSC-INS + cells and true β cells and provides a catalog of genes whose manipulation may convert hPSC-INS + cells into functional β cells. (pnas.org)
  • Since alpha-ketoglutarate is known to change how genes are regulated by removing methyl chemical groups from the DNA in a cell, Teitell and TeSlaa suspected that the molecule was helping cells turn off genes related to pluripotency and turn on genes related to more efficient differentiation. (scienceblog.com)
  • Indeed, the addition of succinate caused the stem cells to differentiate slower and less efficiently, which provided further evidence that alpha-ketoglutarate works by acting on genes. (scienceblog.com)
  • populations
  • Finally, we demonstrate that the aggregation and rotary suspension method can be used to support culture and maintenance of hPSC-derived cell populations representing each of the three germ layers, underscoring the utility of this platform for culturing many different cell types. (ca.gov)
  • Understanding the mechanisms and the extrinsic regulation of cell-fate decisions in stem cell populations and the identification and isolation of pure cell lineages are two major goals of stem biology. (spiedigitallibrary.org)
  • These cell populations were further enriched based on surface markers expression, then recombined allowing development of beating 3D structures termed cardiac microtissues. (biologists.org)
  • progeny
  • We propose solutions to several challenges associated with the consistent formation and extended culture of cell spheroids generated from hPSCs and their differentiated progeny. (ca.gov)
  • These colonospheres, enriched for colon cancer stem cells, were used to establish isogenic cultures of stably differentiated nontumorigenic progeny. (mcponline.org)
  • researchers
  • You will hear from Museum scientists, medical researchers at the frontiers of the field, and a panel of bioethics experts who will address the ethical implications of stem cell research and therapy. (coursera.org)
  • Finally, the researchers have made skin cells. (coursera.org)
  • A jagged little protein appears to be key to how cancer stem cells differentiate and enable metastasis, according to researchers at Rice University and the Duke University School of Medicine. (news-medical.net)
  • The researchers saw similar results when they added alpha-ketoglutarate to other cocktails of molecules that are used to produce other cell types. (scienceblog.com)
  • With LabBag®, researchers have developed an all-in-one system in the form of a transparent bag that provides a cheap, fast and sterile way for scientists to grow, differentiate and freeze stem cells. (bioportfolio.com)
  • Researchers at Case Western Reserve University have discovered how to quickly and accurately predict which lung cancer patients will benefit from chemotherapy by analyzing how immune cells the body sends out to fight the disease are arranged. (technologynetworks.com)
  • Associate professor Dr. Hanna Mikkola reports that her team of researchers have found a link between trophoblasts and premature differentiation of blood stem cells. (doctortipster.com)
  • The study shows a new discovery in the development of blood stem cells whilst offering new findings that could lead to further development of artificial hepatopoietic niches that could help researchers understand the defects that originate from the placenta. (doctortipster.com)
  • cancer stem
  • Duke graduate student Larisa Gearhart-Serna, co-lead author of the paper, used a novel tumor organoid model of inflammatory breast cancer cells that is enriched with cancer stem cells developed previously in Devi's lab to show that reducing the presence of JAG1 affected the cancer stem-like properties and capacity to proliferate. (news-medical.net)
  • Therapy-resistant cancer stem cells have been implicated in drug resistance and tumor recurrence. (mcponline.org)
  • Out of a total data set of 3048 identified proteins, 32 proteins were at least twofold up-regulated in the colon cancer stem cells when compared with the differentiated cells. (mcponline.org)
  • Knockdown of BIRC6 sensitized colon cancer stem cells against the chemotherapeutic drugs oxaliplatin and cisplatin. (mcponline.org)
  • This study reveals that differentiation of colon cancer stem cells is accompanied by altered regulation of cell death pathways. (mcponline.org)
  • Targeting BIRC6, or other Inhibitors of Apoptosis Proteins, may help eradicating colon cancer stem cells. (mcponline.org)
  • Cancer stem cells make up only a few percent of the total tumor cell mass, but are uniquely endowed with tumor-initiating capacity ( 2 ⇓ ⇓ - 5 ). (mcponline.org)
  • scientists
  • Our findings may help overcome that challenge and let scientists more easily create cells to treat disease. (scienceblog.com)
  • A new study conducted by scientists at University of California, Los Angeles (UCLA) finds a new stem cell niche along with a signaling tract, both having an important role in providing a good blood supply during life, whilst preventing any premature differentiation. (doctortipster.com)
  • Scientists previously discovered that the placenta is the place where numerous blood stem cells reside in an undifferentiated state until needed. (doctortipster.com)
  • Stem cell differentiation can now be seen thanks to a combination of machine learning and microfabrication techniques developed by scientists at the RIKEN Quantitative Biology Center in Japan. (riken.jp)
  • murine
  • For example, a murine MSC population isolated from mouse bone marrow and injected into the quadriceps of the mdx mouse demonstrated that the cells could locally contribute dystrophin to the muscle fiber sarcolemma. (ahajournals.org)
  • heterogeneity
  • The team at Duke led by Gayathri Devi, an associate professor in surgery and pathology and program director of the Duke Inflammatory Breast Cancer Consortium, saw evidence of heterogeneity in lab experiments on highly aggressive, inflammatory breast cancer cells. (news-medical.net)
  • This heterogeneity remains a major roadblock to any clinical advances, because no 'magic bullet' can eradicate all types of cells in a tumor,' she said. (news-medical.net)
  • Single-cell phasor FLIM signatures reveal an increased heterogeneity in the metabolic states of differentiating H9 and H1 hESC colonies. (spiedigitallibrary.org)
  • types
  • So it has the potential to make all cell types of the body. (coursera.org)
  • which means that it can make many cell types within the layer. (coursera.org)
  • the number of cell types it can turn into gets fewer and fewer. (coursera.org)
  • into a number of cell types which are unique to that layer. (coursera.org)
  • they have the ability to make all the cell types of the body. (coursera.org)
  • Both these cell types are essential for correct brain function. (wellcomecollection.org)
  • An ever-increasing number of specific cell types can be generated from PSCs, but technical challenges remain in applying these cells in the clinic. (sciencemag.org)
  • Further, we provide examples to demonstrate how aggregation can be used as a tool to select specific subpopulations of cells to create homotypic spheroids, or as a means to introduce multiple cell types to create heterotypic tissue constructs. (ca.gov)
  • This has prompted interest in identifying cell types capable of replenishing the injured myocardium with healthy cells and augmenting heart function. (ahajournals.org)
  • First, the physical space in which the cells are grown has a dramatic impact on the results, causing significant variation in the types of cells into which they differentiate. (technologynetworks.com)
  • Second, classifying the cell types which have developed through manual observation is time consuming. (technologynetworks.com)
  • Both talin (−/−) ES cell mutants formed embryoid bodies, but differentiation was restricted to two morphologically distinct cell types. (rupress.org)
  • bone
  • Such a cell population might be found in the adult bone marrow. (ahajournals.org)
  • 9 When bone marrow cells from a normal male mouse were injected intravenously into the tail vein of affected mdx female mice, skeletal myotubes in the recipients were found to contain Y-positive nuclei. (ahajournals.org)
  • 12 Recently, Bittner et al 13 showed that bone marrow-derived cells could be recruited to both skeletal and cardiac muscle in the mdx mouse, in which these striated muscles undergo continual remodeling. (ahajournals.org)
  • She adds that progress has been made in the past years regarding the presence of niche cells in adult bone marrow, but only now have they discovered the importance of these niches in fetal growth. (doctortipster.com)
  • iPSC
  • These studies demonstrate the feasibility of expanding functionally competent NKT cells via an iPSC phase, an approach that may be adapted for NKT cell-targeted therapy in humans. (jci.org)
  • neuron
  • from a skin cell to a neuron. (coursera.org)
  • Our iPS cells from schizophrenia patients are perhaps a special case, because they included the genomic deletion from chromosome 22, which, through MAPK14, led to the imbalance in neuron/astrocyte development. (eurekalert.org)
  • biology
  • In this five-part online course you will explore the history and basic biology of stem cells, learn about new research techniques, and find out how stem cells could lead to cures for diseases and to individualized medicine. (coursera.org)
  • lineage
  • The current understanding of the pathogenesis of atherosclerosis includes the assignment of a critical role to cells of the monocyte/macrophage lineage. (ahajournals.org)
  • B-D: VSELs were sorted from this gate (R1) as lineage-negative and Sca-1 positive cells (R2), that are also CD45-negative (R3), while HSPC are CD45-positive (R4). (nih.gov)
  • germ
  • which of the three layers known as germ layers the cell will become part of. (coursera.org)
  • Is the Subject Area "Germ cells" applicable to this article? (plos.org)
  • fetal
  • We employ a new method to isolate and profile insulin-expressing cells and conclude that several different hPSC lines generate very similar insulin-expressing cells, cells whose transcripts resemble fetal rather than adult β cells. (pnas.org)
  • i ) Different hPSC lines produce highly similar INS + cells and ( ii ) hPSC-derived INS + (hPSC-INS + ) cells more closely resemble human fetal β cells than adult β cells. (pnas.org)
  • Fetal Bovine Serum, ES Cell-Qualified FBS (Cat. (thermofisher.com)
  • expression
  • 3 6 We demonstrate that ES Mø exhibit properties typical of arterial lesion Mø, including the expression of modified LDL receptors (scavenger receptor [SR]-A, CD36, and CD68), capacity for foam cell formation, secretion of MMPs, and expression of inflammatory cytokines. (ahajournals.org)
  • We conclude that talin is essential for β1 integrin expression and focal adhesion assembly in undifferentiated ES cells, but that a subset of differentiated cells are talin independent for both characteristics. (rupress.org)
  • growth factors
  • 2 After macrophage foam cell formation, Mø are thought to contribute to lesion progression through a variety of interactions with other cells of the artery wall that depend on the elucidation of a host of cytokines and growth factors by cells residing in the intima. (ahajournals.org)
  • mice
  • The stem cells, labeled with lacZ , were injected into the left ventricle of CB17 SCID/ beige adult mice. (ahajournals.org)
  • NOD/SCID mice were inoculated with luc + K562 tumor cells, and cells were allowed to engraft for 3 days before animals were given 1 systemic (intravenous) infusion of NK cells. (nih.gov)
  • B and D: Lung cells from SPC-KO mice transplanted with non-VSELs or HSPC express cytokeratin but not SPC. (nih.gov)