Stem cells are cells nuclei clone cells. Medical search. Frequent questions

A couple of studies show some success in generating early microscopic embryos, but this [study] is the first successful pluripotent stem cell line," said Daley. (the-scientist.com)
This could help researchers identify abnormalities in iPSC differentiation, correct them, and develop pluripotent stem cells that don't harbor tumorigenic qualities and do not require the use of human embryos. (the-scientist.com)
Human cloning involves creating embryos with the intent of implanting them in women to produce children. (boloji.com)
The bill also applies Federal ethical regulations on human subject research and outlaws the transfer of cloned embryos to a woman's uterus or to any artificial womb. (boloji.com)
At the same time, the statement calls for a five-year moratorium on the use of cloning to create human embryos for research purposes. (boloji.com)
While supporting research that would help to determine whether stem cells have therapeutic effects, they point out that those adult stem cells, umbilical cord stem cells, and embryonic stem cells not derived from embryos created for research can be used. (boloji.com)
Researchers reported in Nature on November 22, 2007, that they successfully isolated 2 embryonic stem cell lines from cloned embryos made using cells from the skin of an adult rhesus macaque. (nih.gov)
These scientists destroyed the embryos and derived stem cell lines. (nationalrighttolifenews.org)
Most embryos…formed one or two pronuclei at the time of removal from TSA, whereas a slightly higher portion of embryos cleaved…suggesting that some SCNT embryos did not exhibit visible pronuclei at the time of examination… Most cleaved embryos developed to the eight-cell stage…but few progressed to compact morula…and blastocyst. (nationalrighttolifenews.org)
Activation of embryonic genes and transcription from the transplanted somatic cell nucleus are required for development of SCNT embryos beyond the eight-cell stage…Therefore, these results are consistent with the premise that our modified SCNT protocol supports reprogramming of human somatic cells to the embryonic state. (nationalrighttolifenews.org)
A story in News.Com.Au-which runs stories from several Australian newspapers celebrates the cloning breakthrough because it means no embryos are used in the process! (nationalrighttolifenews.org)
But it is an important step in research because it doesn't require the use of embryos in creating the type of stem cell capable of transforming into any other type of cell in the body. (nationalrighttolifenews.org)
The Los Angeles Times has waded in to the junk biology game, assuring us that no embryos are threatened in human cloning-WHEN THE WHOLE POINT OF HUMAN CLONING IS TO CREATE AN EMBRYO! (nationalrighttolifenews.org)
This also might have explained why cloning is inefficient: only 1-3% of cloned embryos eventually develop into an adult clone. (rupress.org)
Interest in using stem cells from cloned human embryos has revived after success by scientists in the United States and Korea. (bioedge.org)
Nature adds that rogue scientists might implant cloned embryos into wombs to create cloned children, a possibility which is widely condemned. (bioedge.org)
In humans, a major roadblock in achieving successful SCNT leading to embryonic stem cells has been the fact that human SCNT embryos fail to progress beyond the eight-cell stage. (news-medical.net)
They derived several human embryonic stem cell lines from these cloned embryos whose DNA was an exact match to the adult cell that donated the DNA. (news-medical.net)
The Catholic Church has always held that stem-cell research and therapies are morally acceptable, as long as they don't involve the creation and destruction of human embryos. (archstl.org)
The stem cells suits human needs, does not cause harm and can be obtained from both adult and fetal does not conflict with religious beliefs, it has tissues, umbilical cord and early embryos. (who.int)
Unicellular for those cells that are derived from human organisms are primed to replicate (clone) pre-embryos, which seem to have a high themselves by nature. (who.int)
Yamanaka worked to find new ways to acquire embryonic stem cells to avoid the social and ethical controversies surrounding the use of human embryos in stem cell research during the late twentieth and early twenty-first centuries. (asu.edu)
In the ongoing debate about cloning human embryos for research, and about destroying them in order to harvest their stem cells, it is important to keep some basic facts in mind. (actionlife.org)
Last August, President George W. Bush announced his decision banning federal funding for stem-cell research that involved the destruction of living human embryos. (commonwealmagazine.org)
First, while stem-cell experimentation could involve the creation of embryos with the express purpose of destroying them, this is not the only means available for obtaining embryos. (jewishvaluesonline.org)
Thus, there is broad halakhic (Jewish legal) agreement that stem cell research is permitted on "excess" embryos. (jewishvaluesonline.org)
Most (but not all) authorities would forbid the creation of embryos with the express purpose of killing them in the pursuit of stem cell research. (jewishvaluesonline.org)
The controversy over stem cell research is focused specifically on the use of stem cells taken from embryos. (jewishvaluesonline.org)
Researchers there are working on technology that induces human skin cells to change into the kind of stem cells that have been created by embryos. (cbc.ca)
Some argue that the possibility of mimicking stem cells without acquiring them from embryos, side-steps that moral dilemma. (cbc.ca)
Embryonic stem cells come from embryos, embryonic germ cells from testes, and adult stem cells can come from bone marrow. (cbc.ca)
This new method of generating stem cells does not require embryos as starting points and could be used to generate cells from many adult tissues, such as a patient's own skin cells,' said principal author Andras Nagy, senior investigator at Mount Sinai's Samuel Lunenfeld Research Institute. (cbc.ca)
Some prohibit only cloning for reproductive purposes and allow the creation of cloned human embryos for research, whereas others prohibit the creation of cloned embryos for any purpose. (who.int)

After stimulating this cell to begin dividing, in the proper conditions an embryo will develop. (wikipedia.org)
The resulting embryo was then used to create embryonic stem cells, capable of generating every cell type in the body, and the nuclei of these cells were injected into other eggs to produce clones. (newscientist.com)
The fusion ultimately gives rise to a microscopic embryo, from which embryonic stem cells can theoretically be derived. (the-scientist.com)
In the best case, an early embryo consisting of a few cells may form, but these are not capable of giving rise to human life, nor hESCs for therapeutic purposes. (the-scientist.com)
The resulting clone developed into a microscopic embryo, which survived long enough for pluripotent stem cell lines to be derived. (the-scientist.com)
The egg then "reprograms" the adult nucleus so that the cell behaves like an embryo but has the genes of the adult cell. (nih.gov)
When an embryo like this is implanted into a uterus, as with Dolly, the process is called reproductive cloning. (nih.gov)
In another strategy, called therapeutic cloning, the embryo can instead be used to create stem cells that are genetically identical to a patient. (nih.gov)
Since embryonic stem cells have the ability to form virtually any cell type in the body, those taken from a cloned embryo could potentially be used to treat many diseases. (nih.gov)
Both Lillie and Just used Echinarachnius parma for their studies of egg cell membranes and embryo development at the Marine Biological Laboratory (MBL) at Woods Hole, Massachusetts, in the early 1900s. (asu.edu)
Created by Lewis Wolpert in the late 1960s, the model uses the French tricolor flag as visual representation to explain how embryonic cells can interpret genetic code to create the same pattern even when certain pieces of the embryo are removed. (asu.edu)
The early mammalian embryo consists of the extra-embryonic cell layers-the trophoblast and a body of cells called the inner cell mass (ICM), which eventually become the embryo proper. (thefutureofthings.com)
creates a cloned embryo. (nationalrighttolifenews.org)
A cloned embryo-like a natural embryo-is an individual organism, a member of its (in this case, human) species. (nationalrighttolifenews.org)
After that, the question becomes not whether to clone, but what to do with the embryo that was created through the cloning process. (nationalrighttolifenews.org)
… "embryo" means a human organism during the first 56 days of its development following fertilization or creation, excluding any time during which its development has been suspended, and includes any cell derived from such an organism that is used for the purpose of creating a human being. (hinxtongroup.org)
… "human clone" means an embryo that, as a result of the manipulation of human reproductive material or an in vitro embryo, contains a diploid set of chromosomes obtained from a single - living or deceased - human being, fetus, or embryo. (hinxtongroup.org)
It is also our view that there are no sound reasons for treating the early-stage human embryo or cloned human embryo as anything special, or as having moral status greater than human somatic cells in tissue culture. (wikiquote.org)
However, the process is still ethically controversial, as researchers first create a human embryo and then destroy it to create stem cells. (bioedge.org)
It creates an embryo only for the purpose of harvesting its cells. (bioedge.org)
Park Se-pill, head of Seoul-based fertility clinic Maria Biotech, said the tests can silence Hwang's critics who have suggested the dog might be a twin created from a split embryo rather than a clone. (blogspot.com)
That's why Father Pacholczyk, director of education at the National Catholic Bioethics Center in Philadelphia, said that the efforts to help people understand the immorality of embryo reserch, including human cloning, must focus on humanizing the issue and appreciating our own embryonic origins, not just on the desired results of embryonic or other types of stem-cell research. (archstl.org)
After many divisions in culture, this single cell forms a blastocyst (an early stage embryo with about 100 cells) with almost identical DNA to the original donor who provided the adult cell - a genetic clone. (eurostemcell.org)
Hwang and colleagues report that the cells are chromosomally normal, self-renewing and "pluripotent" - meaning they have the ability to form the three major types of cells in the early embryo that give rise to all other cells in the body. (scienceblog.com)
Normally, the embryo comes into being through sexual conception, in which the female egg cell is fertilized by a male sperm cell. (actionlife.org)
Although reasonable people can disagree about the moral status and "personhood" of the embryo, the distinction drawn between therapeutic and reproductive cloning is sophistry. (commonwealmagazine.org)
The real issue is quite straightforward: Those in favor of therapeutic cloning believe that the potential good to be derived from the destruction of the embryo outweighs the fact that human life has been created only to be exploited and then destroyed. (commonwealmagazine.org)
increased public sensitivity and awareness together with the development of national regulations of governance of human cloning and embryo research in general. (lifeissues.net)
3. National regulations of governance of human cloning and embryo research in general adopted so far confirm the convergence of views of the refusal to adopt legislation or guidelines permitting reproductive cloning , while they still show variations on the legitimacy of human cloning carried out as part of research agendas. (lifeissues.net)
In the first 4 - 5 days after fertilization, the early-stage embryo (or blastocyst) is comprised of about 150 cells, within which there is a region called the Inner Cell Mass containing the stem cells. (jewishvaluesonline.org)
The controversy arises for some people because, in the course of harvesting these cells, the embryo is destroyed. (jewishvaluesonline.org)
They isolate skin cells, then using the same procedure that created Dolly, they create an embryo from them. (biologywriter.com)
The egg with its skin cell nucleus is allowed to form a 120-cell embryo. (biologywriter.com)
The embryo is then destroyed, its cells used as embryonic stem cells for transfer to injured tissue. (biologywriter.com)
The mouse experiment carried out by the Rockefeller researchers would have been far more controversial if it had been carried out in humans, because the 120-cell embryo could in principle be brought to term by inserting it into a human uterus. (biologywriter.com)
2. Nuclear transfer is a technique used to duplicate genetic material by creating an embryo through the transfer and fusion of a diploid cell in an enucleated female oocyte.2 Cloning has a broader meaning than nuclear transfer as it also involves gene replication and natural or induced embryo splitting (see Annex 1). (who.int)

This is how Dolly the Sheep and many other species were cloned. (wikipedia.org)
Despite this, the low efficiency of the technique has prompted some researchers, notably Ian Wilmut, creator of Dolly the cloned sheep, to abandon it. (wikipedia.org)
Roger Highfield Dolly creator Prof Ian Wilmut shuns cloning. (wikipedia.org)
Researchers have been hoping to harness the therapeutic potential of cloning ever since the cloning of Dolly the sheep in 1997. (nih.gov)
Dolly the sheep was famously cloned using this method in 1996. (livescience.com)
Then in '97 the paper by Ian Wilmut came out on the cloning of Dolly the sheep. (rupress.org)
This question had not been resolved unequivocally by the cloning of Dolly or other mammals. (rupress.org)
This has led to a lot of interest in SCNT, which is best known as the method used to pioneer whole animal cloning technology, such as Dolly the sheep. (news-medical.net)
It became a hot topic in 1996 when Dolly the sheep was cloned via a process called somatic cell nuclear transfer. (archstl.org)
Cloning, or somatic cell nuclear transfer (SCNT), is the technique used to produce Dolly the sheep, the first animal to be produced as a genetic copy of another adult. (eurostemcell.org)
To produce Dolly, the cloned blastocyst was transferred into the womb of a recipient ewe, where it developed and when born quickly became the world's most famous lamb. (eurostemcell.org)
Yamanaka also noted that experiments in cloning Dolly the sheep in 1996, conducted by Ian Wilmut, Angelica Schnieke, Jim McWhir, Alex Kind, and Keith Campbell at the Roslin Institute in Roslin, Scotland, influenced his work. (asu.edu)
The Dolly experiment showed that scientists could reprogram the nucleus of somatic cells by transferring the contents of the nucleus into oocytes that have had their nuclei removed, a technique called somatic cell nuclear transfer (SCNT). (asu.edu)
He was in high school when he heard about Dolly, one of the world's most extreme examples of cell reprogramming. (lu.se)
Through the cloning of Dolly, we learned that the cell nucleus contains all the genetic information needed for the cell to develop into any type of cell. (lu.se)
Although many species produce clonal offspring in this fashion, Dolly, the lamb born in 1996 at a research institute in Scotland, was the first asexually produced mammalian clone. (who.int)
Somatic-cell nuclear transfer, the technique by which Dolly was created, was first used 40 years ago in research with tadpoles and frogs. (who.int)

As the cell begins to divide, scientists believe stem cells can be extracted and grown into tissue or organs. (boloji.com)
Yesterday, scientists in the United Kingdom announced that they'd been granted permission by the Human Fertilization and Embryology Authority to create stem cells by therapeutic cloning. (sentientdevelopments.com)
In therapeutic cloning, scientists take a human egg from a healthy donor, and remove its nucleus. (sentientdevelopments.com)
Our scientists have developed a wide array of stem cell-focused reagents and resources for many applications including flow cytometry , western blotting , ELISAs , and recombinant proteins for cell differentiation. (biolegend.com)
While regarded by many top scientists as the Holy Grail of medicine, others consider embryonic stem-cell research sacrilegious. (thefutureofthings.com)
A number of large biotech companies and scientists are looking toward stem cells as the basis for a therapeutic solution to cure such illnesses as blindness, diabetes and spinal cord injuries. (thefutureofthings.com)
Scientists have used cloning technology to transform human skin cells into embryonic stem cells, an experiment that may revive the controversy over human cloning. (nationalrighttolifenews.org)
The method described on Wednesday by Oregon State University scientists in the journal Cell, would not likely be able to create human clones, said Shoukhrat Mitalipov, senior scientist at the Oregon National Primate Research Center. (nationalrighttolifenews.org)
Scientists have cloned organisms before, generally by injecting the nucleus of a donor cell into an egg whose own DNA has been removed. (livescience.com)
Scientists are unlikely to rush into creating human embryonic stem cell lines. (bioedge.org)
The con- is removed and replaced by a nucleus of cept of human cloning has long been in the another cell type, the stem cell will then imagination of many scientists, scholars and be reprogrammed to produce the product fiction writers [ 1 ]. (who.int)
Scientists have isolated the first human embryonic stem cell lines specifically tailored to match the nuclear DNA of patients, both males and females of various ages, suffering from disease or spinal cord injury. (scienceblog.com)
The work also moves scientists one step closer to the goal of transplanting healthy cells into humans to replace cells damaged by diseases such as Parkinson's and diabetes. (scienceblog.com)
From the 31 nuclear-transfer blastocysts, the scientists derived 11 stem cell lines. (scienceblog.com)
Yamanaka received the Nobel Prize in Physiology or Medicine in 2012, along with John Gurdon, as their work showed scientists how to reprogram mature cells to become pluripotent. (asu.edu)
Takahashi and Yamanaka's 2006 and 2007 experiments showed that scientists can prompt adult body cells to dedifferentiate, or lose specialized characteristics, and behave similarly to embryonic stem cells (ESCs). (asu.edu)
If one of the infected cells showed G418 resistance, then the scientists would know that one of the twenty-four genes influenced the cell to become an embryonic stem cell-like cell. (asu.edu)
When scientists at the Oregon Health and Science University announced success in performing SCNT with human cells last year, it reignited interest in eventually using the technique for human therapies. (pharmaceuticalintelligence.com)
Because the early stem cells have the ability to become any one of the hundreds of different kinds of human cells, scientists are working on research using these cells with the aim of creating therapies to treat a variety of diseases. (jewishvaluesonline.org)
Scientists have been all abuzz in the last few years over stem cells - cellular magicians that promise to dazzle and amaze. (cbc.ca)
Scientists say embryonic stem cells are the most useful type because they have the potential to become any type of cell within the body. (cbc.ca)
Scientists are fascinated by the ability of stem cells to become any type of cell. (cbc.ca)
By surgically transplanting embryonic stem cells, scientists have performed the remarkable feat of repairing disabled body tissues in mice. (biologywriter.com)
Scientists were initially interested in somatic-cell nuclear transfer as a means of determining whether genes remain functional even after most of them have been switched off as the cells in a developing organism assume their specialized functions as blood cells, muscle cells, and so forth. (who.int)
1. Cloning is an umbrella term traditionally used by scientists to describe different processes for duplicating biological material. (who.int)
The Cellomics Arrayscan VTI instrument is an imaging tool that will help our scientists make quicker and more precise analyses, capturing, for instance, changes in cell size, shape and intensity. (lu.se)

Here, we employed a human pancreatic differentiation platform complemented with an shRNA screen in human pluripotent stem cells (PSCs) to identify potential drivers of early endoderm and pancreatic development. (mdpi.com)
For the first time researchers can now compare iPSC differentiation to the same process an egg goes through after the transfer of a somatic cell genome. (the-scientist.com)
RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. (biolegend.com)
These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)
The term stem cell can be defined by two very important qualities: the cell has the ability to self-renew and, in a more general sense, the cell has not completed differentiation into its final state. (thefutureofthings.com)
This general definition includes a wide variety of cells with varying degrees of differentiation potential. (thefutureofthings.com)
Transferring a terminally differentiated cell nucleus into an egg cell that has had its own nucleus removed wipes away the epigenetic marks of differentiation and allows the nucleus once again to code for any cell type. (rupress.org)
In addition, researchers must develop methods to efficiently direct the differentiation of embryonic stem cells to specific stable cell types. (scienceblog.com)
Drs. John B. Gurdon, Irving L. Weissman, and Shinya Yamanaka have been pioneers in studying stem cells and the reprogramming of highly differentiated adult cells into pluripotent cells capable of directing differentiation from a single cell to an adult animal. (brandeis.edu)
No differentiation and self-organization into multi-cellular bile ductules, as observed in the parental PICM-19 cell line, occurred within the PICM-19H cell monolayer even after six months of maintenance culture. (usda.gov)
Additionally, lingering expression or re-expression of viral transgenes in iPSC-derived cells can interfere with their differentiation potential. (addgene.org)
MDS affects hematopoiesis at the stem cell level, as indicated by cytogenetic abnormalities, molecular mutations, and morphologic and physiologic abnormalities in maturation and differentiation of one or more of the hematopoietic cell lines. (medscape.com)
Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). (bvsalud.org)
Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. (bvsalud.org)
Since the Notch signaling pathway molecules play an important role in differentiation of epithelial cells, it is important to identify the presence of notch signaling molecules in SHED during the process of cell differentiation. (bvsalud.org)
The Notch signaling pathway provides important intercellular signaling mechanisms essential for cell fate specification and it regulates differentiation and proliferation of stem or progenitor cells by para-inducing effects 3-4 . (bvsalud.org)
Notch signaling pathway is also involved in the regulation of epithelial cell differentiation in various tissues 5-6 . (bvsalud.org)
These malignancies are further characterized by the maturity and differentiation of the individual cell types and are divided into acute leukemias such as acute myeloid leukemia and acute lymphoblastic leukemia and chronic leukemias such as chronic myeloid leukemia and chronic lymphocytic leukemia. (medscape.com)
Different characteristics of the nuclei and cytoplasm of the cell allow differentiation by instrumentation and microscopy. (medscape.com)
First, we utilized single cell sequencing to dissect the differentiation of stem cells to midbrain dopaminergic neurons. (lu.se)

A blastocyst (cloned or not), because it lacks any trace of a nervous system, has no capacity for suffering or conscious experience in any form - the special properties that, in our view, spell the difference between biological tissue and a human life worthy of respect and rights. (wikiquote.org)
In therapeutic cloning, the blastocyst is not transferred to a womb. (eurostemcell.org)
Instead, embryonic stem cells are isolated from the cloned blastocyst. (eurostemcell.org)
Stem cells were then derived from the inner cell mass of the blastocyst. (scienceblog.com)
The Korean researchers who performed this stem cell research improved upon their protocols that yielded the first embryonic stem cell line from a cloned human blastocyst. (scienceblog.com)

The cell contains genetic information identical to the donated somatic cell. (wikipedia.org)
Recently, the importance of telomere maintenance in human stem cells has been highlighted by studies on dyskeratosis congenital, which is a genetic disorder in the human telomerase component. (nature.com)
Globally, there are several cloning programmes that aim to increase the size of rapidly-dwindling populations of endangered species such as African wildcats, and maintain genetic diversity through one-off clonings of individuals that haven't bred . (newscientist.com)
The findings validate this controversial method, and may one day allow therapeutic stem cells to be created from a patient's own genetic material. (the-scientist.com)
Noggle said the findings may also pave the way for better induced pluripotent stem cells (iPSCs), which form when somatic cells are regressed to a pluripotent state through the use of genetic factors. (the-scientist.com)
In therapeutic cloning on the other hand, genetic material from a body cell is inserted into an egg cell, replacing the nucleus. (boloji.com)
The French flag model represents how embryonic cells receive and respond to genetic information and subsequently differentiate into patterns. (asu.edu)
The key is that the DNA, the genetic material in those embryonic stem cells, comes from the patient, whose immune system won't reject the stem cells. (sentientdevelopments.com)
The university has been conducting a probe on Hwang and his research since Dec. 16, including genetic tests on stem cells being stored at the laboratories. (blogspot.com)
These stem cells are genetically matched to the donor organism, holding promise for studying genetic disease. (eurostemcell.org)
Each of the 11 new human embryonic stem cell lines was created by transferring the nuclear genetic material from a non-reproductive cell of a patient into a donated egg, or "oocyte," whose nucleus had been removed. (scienceblog.com)
In their new paper, Science author Woo Suk Hwang from Seoul National University in Seoul, Korea and colleagues replaced the nuclei from donated oocytes with nuclei from skin cells from male and female patients, ranging in age from 2 to 56, who had spinal cord injuries, juvenile diabetes and the genetic disease "congenital hypogamma-globulinemia. (scienceblog.com)
From the 185 donated oocytes, endowed with the genetic material from a different person (or in one case, the same person), the researchers report development of 31 hollow balls of cells called "human nuclear-transfer blastocysts. (scienceblog.com)
They called the pluripotent stem cells that they produced induced pluripotent stem cells (iPSCs) because they had induced the adult cells, called differentiated cells, to become pluripotent stem cells through genetic manipulation. (asu.edu)
In 2004, Yamanaka began working at Kyoto University as a professor, where he studied factors that help an organism fend off retroviruses, which are single-stranded RNA viruses that can incorporate their genetic material into the DNA of a host cell. (asu.edu)
Similarly, a clone would be a genetic duplicate of another human being, but there is no denying that it would also be a separate individual. (actionlife.org)
In 1962 he made the stunning observation that it was possible to take a differentiated adult cell from a frog and to re-set its genetic program so that the reprogrammed nucleus could be implanted in an enucleated egg and direct the development of tadpoles. (brandeis.edu)
One attraction of SCNT has always been that the genetic identity of the new pluripotent cell would be the same as the patient's, since the transplanted nucleus carries the patient's DNA. (pharmaceuticalintelligence.com)
But what is not getting such wide reporting is the use of pluripotent stem cells (as well as many other types of cells and genetic engineering techniques) for reproductive purposes . (lifeissues.net)
Cloning is often confused with other advances in bio-medicine and bio-engineering - such as genetic selection. (earthtomarrakech.org)
As part of its charge, the committee was asked to prepare a subreport evaluating methods for detecting potential unintended compositional changes across the spectrum of messenger ribonucleic acid (mRNA), proteins, metabolites and nutrients that may occur in food derived from cloned animals that have not been genetically modified via genetic engineering methods. (nationalacademies.org)
Most natural cloning occurs in those species that produce their descendants asexually, that is, without combining the male and female genetic material. (who.int)
if it implants and the pregnancy goes to term, the resulting individual will carry the same nuclear genetic material as the donor of the adult somatic cell. (who.int)
However, an animal created through this technique would not be a precise genetic copy of the source of its nuclear DNA because each clone derives a small amount of its DNA from the mitochondria of the egg (which lie outside the nucleus) rather than from the donor of cell nucleus. (who.int)
A clone is an organism that is a genetic copy of an existing one. (who.int)

In addition to their ability to supply cells at the turnover rate of their respective tissues, they can be stimulated to repair injured tissue caused by liver damage, skin abrasions and blood loss. (thefutureofthings.com)
The ability of our body to regenerate some of its tissues is largely owed to the reserves of adult stem cells. (thefutureofthings.com)
This long-sought technique may eventually let doctors create replacement cells for a wide variety of tissues from bits of a patient's own skin. (nationalrighttolifenews.org)
Cloning of human cells is a technology that holds the potential to cure many diseases and provide a source of exactly matched transplant tissues and organs. (news-medical.net)
Dec. 29, 2005 -- South Korean laboratories used by scientist Hwang Woo Suk no longer have any stem cells created from patients' tissues, the result of the researcher's landmark May 2005 paper, the Seoul National University said. (blogspot.com)
CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. (thermofisher.com)
Mouse cells and tissues created through nuclear transfer can be rejected by the body because of a previously unknown immune response to the cell's mitochondria, according to an international study in mice by researchers at the Stanford University, MIT and colleagues in Germany and England. (pharmaceuticalintelligence.com)
The hope has been that this would eliminate the problem of the patient's immune system attacking the pluripotent cells as foreign tissue, which is a problem with most organs and tissues when they are transplanted from one patient to another. (pharmaceuticalintelligence.com)
In the normal course of gestation, these cells will divide and split off from one another to become every cell in the human body, forming the various organs and tissues. (jewishvaluesonline.org)
The basic strategy for repairing damaged tissues is to surgically transfer embryonic stem cells to the damaged area, where the stem cells can form healthy replacement cells. (biologywriter.com)
Therapeutic cloning successfully addresses the key problem that must be solved before stem cells can be used to repair human tissues damaged by heart attack, nerve injury, diabetes, or Parkinsons, which is immune acceptance. (biologywriter.com)
In theraputic cloning, the body readily accepts stem cells because they are cloned from its own tissues, and so pass the immune system s self identity check. (biologywriter.com)
Chemokines," which take their name from chemotactic cytokines, are small secreted polypeptides that regulate movement of immune cells into tissues (Baggiolini et al. (justia.com)
18:217-242), the β-chemokines regulate the expression of adhesion molecules on endothelial cells and thereby indirectly affect diapedesis and extravasation of immune cells from the circulation into tissues. (justia.com)
Other shared functions of MCP-1, MCP-2, and MCP-3 include induction of N-acetyl β-D-glucosaminidase release, gelatinase B release, and granzyme A release which are believed to help the cells digest the extracellular matrix components necessary to enable them to migrate into tissues (Van Coillie et al. (justia.com)
Stem cells from human exfoliated deciduous teeth (SHED) are multipotent stem cells derived from the pulp tissues of extracted deciduous teeth 1 . (bvsalud.org)
genic models are inadequate for number of activated CD8-positive T LMP1 was strongly expressed in the understanding the cancer etiology in cells increased considerably in the lymphoma tissues but was hardly the context of natural viral infection. (who.int)

Before this new study was published, Nature asked another group of researchers to confirm that the stem cells were genetically identical to the donor skin cells. (nih.gov)
But they showed, for the first time, that it is possible to create cloned embryonic stem cells that are genetically identical to the person from whom they are derived. (nationalrighttolifenews.org)
In biology , cloning is the process of producing similar populations of genetically identical individuals that occurs in nature when organisms such as bacteria , insects or plants reproduce asexually . (wikiquote.org)
Developments in biotechnology have raised new concerns about animal welfare, as farm animals now have their genomes modified (genetically engineered) or copied (cloned) to propagate certain traits useful to agribusiness, such as meat yield or feed conversion. (wikiquote.org)
There have been many false dawns in the field of embryonic stem cell research, but these results seem to confirm that it is possible to use adult cells to create genetically matched stem cell lines. (bioedge.org)
By transferring adult cell DNA into an embryonic stem cell, it is possible to create a line of immortal embryonic cells that are able to develop into any type of adult cell, genetically identical to the donor. (news-medical.net)
Another long-term hope for therapeutic cloning is that it could be used to generate cells that are genetically identical to a patient. (eurostemcell.org)
The team used cells that were created by transferring the nuclei of adult mouse cells into enucleated eggs cells from genetically different mice. (pharmaceuticalintelligence.com)
Thus, the clone would be genetically identical to the nucleus donor only if the egg came from the same donor or from her maternal line. (who.int)
Beyond this scientific interest, the commercial concern in animal cloning focuses on replicating large numbers of genetically identical animals, especially those derived from a progenitor that has been modified genetically. (who.int)

The two entities fuse to become one and factors in the oocyte cause the somatic nucleus to reprogram to a pluripotent state. (wikipedia.org)
Wakayama speculates that freezing and thawing the tissue somehow makes it easier to "reprogram" the brain cell nucleus. (newscientist.com)
Two separate research teams have figured out how to "reprogram" cells with just a handful of genes to give them the characteristics of embryonic stem cells. (nih.gov)
The therapeutic potential of cloned human cells has been demonstrated by another study using human oocytes to reprogram adult cells of a type 1 diabetic. (news-medical.net)
Therefore, new ways are needed to reprogram other cells into immune cells with the same capacity as the "natural" dendritic cells. (lu.se)
In the next step, he used the same molecules to reprogram cancer cells into dendritic cells. (lu.se)

If the cloned cells are placed in the uterus of a female mammal, a cloned organism develops to term in rare instances. (wikipedia.org)
At the top of the list comes the zygote-a fertilized egg, which of course has the ability to divide and differentiate into all cell types in the body and create a new organism. (thefutureofthings.com)
The first three divisions of the zygote give birth to eight totipotent cells, each of which also has the ability to become an entire organism. (thefutureofthings.com)
XI - embryonic stem cells: embryonic cells that are capable of modifying the cells of any organism tissue. (hinxtongroup.org)
This is cloning, a process in which the body cell that donated the replacement nucleus supplies the chromosomes of the new human organism. (actionlife.org)
Whether the new organism is produced by fertilization or by cloning, each new human organism is a distinct entity. (actionlife.org)

Somatic Cell Nuclear Transfer (SCNT) is the process by which the nucleus of an oocyte (egg cell) is removed and is replaced with the nucleus of a somatic (body) cell (examples include skin, heart, or nerve cell). (wikipedia.org)
체세포 핵 치환 (Somatic-cell nuclear transfer, SCNT)은 난자 의 핵 을 제거한 후에, 체세포 의 핵을 이식하여 복제 를 하는 기술을 말한다. (wikipedia.org)
Once the SCNT is done, the cloning is over. (nationalrighttolifenews.org)
The cloning is completed when the SCNT is accomplished. (nationalrighttolifenews.org)
One cloning technology that has been developed for mammalian and human cells is somatic cell nuclear transfer (SCNT). (news-medical.net)
SCNT is a method of cloning mammalian cells that can be used to create personalized embryonic stem cells from an adult animal or human. (news-medical.net)
But SCNT can also be used to clone human cells for transplant or other therapies. (news-medical.net)
This was the first successful reprogramming of human somatic cells into embryonic stem cells using a cloning technique, SCNT. (news-medical.net)
Another successful attempt at human SCNT was made using cells from two adult males. (news-medical.net)
This method is called "somatic cell nuclear transfer" or SCNT. (scienceblog.com)
The promise of the SCNT method is that the nucleus of a patient's skin cell, for example, could be used to create pluripotent cells that might be able to repair a part of that patient's body. (pharmaceuticalintelligence.com)
Stanford University have raised the possibility in the past that the immune system of a patient who received SCNT-derived cells might still react against the cells' mitochondria, which act as the energy factories for the cell and have their own DNA. (pharmaceuticalintelligence.com)
This reaction could occur because cells created through SCNT contain mitochondria from the egg donor and not from the patient, and therefore could still look like foreign tissue to the recipient's immune system. (pharmaceuticalintelligence.com)
When transplanted back into the nucleus donor strain, the cells were rejected although there were only two single nucleotide substitutions in the mitochondrial DNA of these SCNT-derived cells compared to that of the nucleus donor. (pharmaceuticalintelligence.com)
Although many stem cell researchers are focused on a different method of creating pluripotent stem cells, called induced pluripotent stem cells, there may be some applications for which SCNT-derived pluripotent cells are better suited. (pharmaceuticalintelligence.com)
The immunological reactions reported in the new paper will be a consideration if clinicians ever use SCNT-derived stem cells in human therapy, but such reactions should not prevent their use. (pharmaceuticalintelligence.com)
This research informs the medical community of the margin of safety that would be required if, in the distant future, researchers need to use SCNT to create pluripotent cells to treat someone. (pharmaceuticalintelligence.com)

Instead of removing the egg genome prior to nuclear transfer, he and his colleagues added the somatic cell nucleus directly to the intact egg. (the-scientist.com)

The step involves removing the DNA from an oocyte (unfertilised egg), and injecting the nucleus which contains the DNA to be cloned. (wikipedia.org)
Somatic cell nuclear transfer typically involves the transfer of genomic information from a somatic cell into an unfertilized egg cell whose nucleus has been removed. (the-scientist.com)
Currently, the procedure for isolating non-reproductive cells for the nuclear transfer method involves animal enzymes and serum. (scienceblog.com)
One method of creating pluripotent stem cells is called somatic cell nuclear transfer, and involves taking the nucleus of an adult cell and injecting it into an egg cell from which the nucleus has been removed. (pharmaceuticalintelligence.com)
A few years ago, in an article in the The Times of London newspaper, the author, Michael Gove, made the following statement: "Embryonic stem-cell experimentation involves not just the destruction of human life but the creation of life with the specific intent to destroy it. (jewishvaluesonline.org)
Pereira's research also involves reprogramming skin cells into dendritic cells, which are the sentinels of the immune system. (lu.se)
subtypes of cutaneous T-cell lymphoma, SS is a malignant neoplasm originating the most common of which are mycosis from T lymphocytes, which involves the fungoides and Sézary syndrome (SS). (bvsalud.org)

They put the nucleus of the patient's cell into the egg whose nucleus has been removed. (sentientdevelopments.com)

The potential of therapeutic cloning for treating, and perhaps curing, a variety of debilitating diseases demands that the scientific community be allowed to continue this promising work. (boloji.com)

It was thought that mammalian cells might be refractory to cloning. (rupress.org)
Lentivirus can effectively transduce both dividing and non-dividing mammalian cells, and integrate into the host genome, allowing stable long-term, high-level gene expression both in vivo and in vitro. (onenucleus.com)

Elaboration of an international convention against reproductive cloning of human beings has been under consideration in the United Nations since December 2001 when the subject was included in the agenda of the fifty- sixth session as a supplementary agenda item at the request of France and Germany. (who.int)
2. Over the years, the international community has tried without success to build a consensus on an international convention against the reproductive cloning of human beings. (who.int)
3. Creating awareness among ministries of health in the African Region will provide them with critical and relevant information on the reproductive cloning of human beings and its implications to the health status of the general population. (who.int)
7. The WHO Regional Committee for Africa is invited to review this document for information and guidance concerning reproductive cloning of human beings. (who.int)
3. Media reports on nuclear transfer are usually about one form, reproductive nuclear transfer, also known as reproductive cloning of human beings . (who.int)

After examination, Takahashi and Yamanaka concluded that the cells were similar to embryonic stem cells and duplicated themselves in similar periods of as embryonic stem cells. (asu.edu)
skin cells of the mouse into cells strikingly similar to embryonic stem cells, capable of development into a mouse. (brandeis.edu)

In rare instances, the newly constructed cell will divide normally, replicating the new DNA while remaining in a pluripotent state. (wikipedia.org)
The advance here is the proof that somatic cell nuclear transfer can work [in human cells] and can fully reset the donor cell genome to a pluripotent state," said Harvard Medical School's George Daley , who was not affiliated with the study. (the-scientist.com)
An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. (thermofisher.com)
Direct neuronal reprogramming of a somatic cell into therapeutic neurons, without a transient pluripotent state, provides new promise for the large number of individuals afflicted by neurodegenerative diseases or brain injury. (lu.se)

General Assembly the following year,3 and the World Medical Association's Resolution on Cloning, endorsed in 1997, have confronted the issue but lack binding legal force. (who.int)
WHA50.37 of 1997 argues that human cloning is ethically unacceptable and contrary to human integrity and morality. (who.int)

Thus, a kind of 'regenerative medicine' gives people access to therapies derived from their own cells. (boloji.com)
However, the Senate bill does allow for therapeutic cloning, known as 'nuclear transplantation', for research on therapies that could cure several serious and life-threatening diseases. (boloji.com)
These cells have been sought after as potential therapies for diseases ranging from heart disease to Parkinson's to cancer. (news-medical.net)
The Church also supports research and therapies using adult stem cells, which are cells that come from any person who has been born - including umbilical cord blood, bone marrow, skin and other organs. (archstl.org)
His demonstration that the expression of four master regulatory genes was sufficient to cause the reprogramming of adult cells has opened up many possibilities for human stem cell therapies. (brandeis.edu)
Stem cell therapies hold vast potential for repairing organs and treating disease. (pharmaceuticalintelligence.com)
The use of various types of stem cells for research purposes to make disease "models" in the lab for regenerative medicine and for "therapies" to cure sick patients for diseases is constantly in the news. (lifeissues.net)
For instance, it may be possible one day to produce cardiac tissue to repair a heart damaged in a heart attack, nerve tissue to repair spinal cord injuries and cell therapies to treat people suffering from Alzheimer's or ALS. (jewishvaluesonline.org)
In May 2007, Ontario and California announced a $30-million stem cell research deal aimed at finding new therapies for those diseases. (cbc.ca)
The data presented in this thesis may serve as valuable resources to help optimize future cell replacement therapies for patients suffering from PD. (lu.se)

Potentially easier would be cloning cryogenically frozen humans, though the consensus among cloning experts is that it would be unethical and dangerous to clone a human. (newscientist.com)
Before patient-specific stem cells can potentially be used in the clinic, a variety of issues must be addressed, the researchers emphasized. (scienceblog.com)
The advent of techniques to propagate animals by nuclear transfer, also known as cloning, potentially offers many important applications to animal agriculture, including reproducing highly desired elite sires and dams. (nationalacademies.org)
Reprogramming skin cells to blood stem cells could potentially provide an unlimited source of cells for transplantation to patients with blood disorders. (lu.se)
This approach could be potentially applied directly in the brain by targeting resident cells as a source of new neurons. (lu.se)

Nuclear transfer is a form of cloning. (wikipedia.org)
Nuclear transfer is a delicate process that is a major hurdle in the development of cloning technology. (wikipedia.org)
A breakthrough in somatic cell nuclear transfer opens the possibility of producing human embryonic stem cells with a patient's own genes. (the-scientist.com)
The first pluripotent human embryonic stem cells (hESCs) have been generated from somatic cell nuclear transfer, according to a study published today (October 5) in Nature . (the-scientist.com)
Somatic cell nuclear transfer has shown limited success in animal studies, which have successfully isolated pluripotent cells. (the-scientist.com)
In humans, somatic transfer has been less fruitful-the egg cell quits dividing and often dies after nuclear transfer. (the-scientist.com)
More than 100 nuclear transfer procedures could be required to produce one viable clone. (wikiquote.org)
Nuclear transfer is one of the most sophisticated, fickle, and challenging techniques in cell biology. (rupress.org)
For example, stem cells could be generated using the nuclear transfer process described above, with the donor adult cell coming from a patient with diabetes or Alzheimer's. (eurostemcell.org)
The single cell line generated in the 2004 Science paper resulted from nuclear transfer in which the oocyte and non-reproductive ("somatic") cell came from the same healthy female. (scienceblog.com)
The ten additional new lines resulted from nuclear transfer with skin cells of males or females and oocytes from biologically-unrelated females. (scienceblog.com)

Adult stem cells can be used to accelerate bone or tendon healing , and they can induce cartilage progenitor cells to produce a better matrix and repair cartilage damage . (thefutureofthings.com)
The first part of the thesis (Paper I, II, III) shows the development and improvement of a hESC-based system of for virus-mediated direct reprogramming of human glial progenitor cells into both induced dopaminergic neurons (iDANs) and GABAergic interneurons. (lu.se)

They used adult cells, but it was possible that the cells that gave rise to successfully cloned animals were derived from rare adult stem cells. (rupress.org)
A Korean institute Wednesday, Dec. 27, said that its DNA tests proved Prof. Hwang Woo-suk at Seoul National University (SNU) had successfully cloned a dog. (blogspot.com)
concept of animal cloning, which has now been successfully carried out with sheep and a number of other mammals. (brandeis.edu)

He was the first to appreciate the therapeutic potential of stem cell therapy and has pioneered its development. (brandeis.edu)

Basically, given the difference of telomere and telomerase activity in human and mouse cells, the telomere and telomerase status in stem cell populations is different between humans and mice ( Harrington, 2004 ). (nature.com)
In rodents, and even in some preliminary trials in humans, human embryonic stem cells have been shown to bridge gaps in spinal cord injuries , allowing restoration of motor functions. (thefutureofthings.com)
In most countries, it is illegal to attempt reproductive cloning in humans. (eurostemcell.org)
One exception is hu- humanized SCID mice, the use of al oncogenic viruses that are strictly man T-cell lymphotropic virus type 1 surrogate hosts has not proven very species-specific, causing cancer in (HTLV-1): in addition to its ability to useful for defining tumour site con- humans only. (who.int)
that is histopathological y very simi- T cel s, B cel s, natural kil er cel s, LMP1 of EBV can transform ro- lar to that caused by hepatitis B vi- macrophages, and dendritic cells, dent fibroblasts and is expressed rus (HBV) in humans, but it does so and this humanized mouse model in most of the human cancers as- through a different mechanism. (who.int)

Dr. Irving L. Weissman is professor of pathology and developmental biology at the Stanford University School of Medicine , where he is director of the Institute of Stem Cell Biology and Regenerative Medicine. (brandeis.edu)

Cloning in biotechnology refers to processes used to create copies of DNA fragments ( molecular cloning ), cells (cell cloning), or organisms . (wikiquote.org)
The term applies not only to entire organisms but also to copies of molecules (such as DNA) and cells. (who.int)

Bone marrow cells display aberrant morphology and maturation (dysmyelopoiesis), resulting in ineffective blood cell production. (medscape.com)
MDS develops when a clonal mutation predominates in the bone marrow, suppressing healthy stem cells. (medscape.com)
Decreased WBC count, leukopenia, is seen when supply is depleted by infection or treatment such as chemotherapy or radiation therapy, or when a hematopoietic stem cell abnormality does not allow normal growth/maturation within the bone marrow, such as myelodysplastic syndrome or leukemia. (medscape.com)
These are the malignant proliferations of abnormal clones of white blood cells within the bone marrow that are broadly categorized into lymphoid and myeloid neoplasms depending on the type of white cell proliferation. (medscape.com)
A bone marrow smear was performed, in which 'cerebriform' cells were identified, confirming the diagnosis of Sézary syndrome. (bvsalud.org)

The Wakayama team used a modified version of a cloning technique in which the nucleus of a mouse cell - in this case a cell from dead tissue that has been frozen and then thawed - is injected into a mouse egg that has had its nucleus removed. (newscientist.com)
In a surprise finding, the Wakayama team discovered that it was easiest to create clones from brain tissue, even though clones have never been created from living brain cells. (newscientist.com)
Brain tissue is also high in sugars, which can protect cells when they freeze. (newscientist.com)
But this is the first time animals have been cloned from lumps of tissue frozen without the use of chemicals that might protect the cells from damage. (newscientist.com)
If there are intact cells in this tissue they have been 'stored' frozen. (wikiquote.org)
One of the greatest controversies triggered tissue, a stem cell encoding for heart tissue by the rapid pace of evolution in biology, will eventually develop into heart tissue particularly in genomics and biotechnology, and so on. (who.int)
Instead, PICM-19H cells looked exclusively like hepatocytes, the main cell type that forms liver tissue. (usda.gov)
and altering cell and tissue characteristics for biomedical research and manufacturing. (nationalacademies.org)
In a study published in the online journal Nature on March 1, 2009, Canadian researches described a new method for generating stem cells from adult human tissue. (cbc.ca)
Transplantations of fetal tissue in the 1980s and 1990s provided proof-of-concept for the potential of cell replacement therapy for PD and some patients benefitted greatly from their transplants. (lu.se)
However, post-mortem analysis of transplanted tissue revealed accumulation of pathological Lewy bodies in a small subset of transplanted cells over time, revealing a host-to-graft disease propagation. (lu.se)
The Cellomics machine allows you to not only image live cells, but to keep those cells alive for both short- and long-term experiments in a controlled environment similar to that of a tissue culture incubator. (lu.se)

Retrieved on December 04, 2023 from https://www.news-medical.net/life-sciences/Cloning-Human-Cells.aspx. (news-medical.net)

Currently unidentified reprogramming factors present in oocytes are capable of initiating a cascade of events that can reset the mature, specialized cell back to an undifferentiated, embryonic state. (wikipedia.org)
The cloning method is based on the fact that cytoplasmic factors in mature, metaphase II oocytes are able to reset the identity of a transplanted adult cell nucleus to an embryonic state. (news-medical.net)

Healthy mice have been cloned from cells from dead mice that had been frozen for 16 years, raising the possibility that endangered species could be cloned from old carcasses that have been tossed in freezers, rather than from living cells frozen using elaborate techniques. (newscientist.com)
Other teams have already cloned mice from previously frozen dead cells . (newscientist.com)
RUNX1-deficient mice fail to generate hematopoietic stem cells. (biolegend.com)
In 2006, Kazutoshi Takahashi and Shinya Yamanaka reprogrammed mice fibroblast cells, which can produce only other fibroblast cells, to become pluripotent stem cells, which have the capacity to produce many different types of cells. (asu.edu)
Each gene was inserted near the mouse Fbx15 gene, a gene that embryonic stem cells express during development in mice. (asu.edu)
In 1988 he first identified and isolated the blood-forming stem cells from mice and went on to define the stages of development between the stem cells and differentiated cells of the immune system. (brandeis.edu)
Embryonic stem cells of mice have been induced to become insulin-secreting pancreas cells. (biologywriter.com)
While exciting, these advances in stem cell research were all done in strains of mice without functioning immune systems. (biologywriter.com)
This prevents the mice from rejecting transplanted stem cells as foreign. (biologywriter.com)
Inoculation with a high dose strains of LMP1 transgenic mice vide a powerful tool in mechanistic of EBV caused a B-cell lymphopro- were established that express LMP1 studies on the role of individual viral liferative disorder in these mice, under the control of the immunoglob- genes in cancer. (who.int)

Human pluripotent stem cells, with their ability to proliferate indefinitely and to differentiate into virtually all cell types of the human body, provide a novel resource to study human development and to implement relevant disease models. (mdpi.com)
Stem cells are unique due to their ability to limitlessly self-renew and differentiate into each cell type in the adult body. (biolegend.com)
The capability of these cells to differentiate depends on the stem cell type, the regulation of gene expression by various transcription factors and interaction with the stem cell niche 1,4 . (biolegend.com)
Transcription factors have an important role in the ability of a cell to self-renew and also differentiate into most cell types, also known as pluripotency 1 . (biolegend.com)
As the embryonic cells divide and the daughter cells differentiate, they become increasingly specific. (thefutureofthings.com)
The ICM continues to differentiate into three germ layers-ectoderm, mesoderm and endoderm, each of which follows a specific developmental destiny that takes them along an ever-specifying path at which end the daughter cells will make up the different organs of the human body. (thefutureofthings.com)
These adult stem cells are considered multipotent, having the ability to differentiate into different cell types, albeit with a more limited repertoire than embryonic stem cells. (thefutureofthings.com)
A particular field encouraged by the foundation is stem-cell research, with the great hope that it will result in the ability to get cells to differentiate into neurons and support cells to bridge the gap of a spinal cord injury. (thefutureofthings.com)
The have been applied to both the plant and ani- stem cells possess pluripotential charac- mal kingdoms without even stirring a ripple teristics, and can differentiate into various of concern in international conscience [ 2 ]. (who.int)
For example, the stem cells can differentiate into cells that display characteristics of skin and retina cells, muscle cell bundles, bone matrix cells and cells of the gastrointestinal and respiratory lining. (scienceblog.com)
The generation of iPSCs is relatively simple in concept: ectopically express a cocktail of stem cell reprogramming factors and wait for cells to de-differentiate. (addgene.org)
SHED was able to differentiate into epithelial like cells when cultured in keratinocyte growth medium (KGM) 2 . (bvsalud.org)

In this procedure, the nucleus of an egg cell is removed and replaced by the nucleus of a cell from another adult. (eurostemcell.org)
Instead, an egg has its nucleus removed and replaced by a nucleus from another type of cell-a body cell. (actionlife.org)

Stem cells can be extracted 5-6 days later and used for research. (wikipedia.org)
American feminists and women's health activists are debating on the difficult issue of human cloning and stem cell research. (boloji.com)
The Society for Women's Health Research, a non-profit group, agrees that therapeutic cloning should be allowed. (boloji.com)
Now the U.K. is positioned to lead the world in translating the potential benefits of stem-cell research into patients. (sentientdevelopments.com)
Stem cells are at the forefront of medical research and incite some of the most controversial ethical and religious debates worldwide. (thefutureofthings.com)
Recent advances in the field of stem-cell research are giving hope to millions. (thefutureofthings.com)
US researchers have reported a breakthrough in stem cell research, describing how they have turned human skin cells into embryonic stem cells for the first time. (nationalrighttolifenews.org)
And this week, the New York Stem Cell Foundation Research Institute announced in Nature that it had created a line of cells from a woman with Type 1 diabetes. (bioedge.org)
Snuppy was cast under suspicion following revelations that the Korean scientist had fabricated his stem cell research. (blogspot.com)
It brightens the prospects that his team retains the source technologies for stem cell research," Park said. (blogspot.com)
``The patient-matching stem cells no longer exist,'' Roe Jung Hye, dean of research of affairs at the university, said in an e-mailed statement. (blogspot.com)
A decade later, cloning came to the forefront in Missouri with the narrow passage of Amendment 2, a ballot initiative in 2006 that constitutionally protects embryonic stem-cell research and human cloning. (archstl.org)
So when I was recently contacted by an earnest and amiable member of a local school board who was concerned about the questionable manner in which the issue of "stem cell" research - both human embryonic and adult - was presented to the high school students in his district in a currently-used science textbook, I agreed to evaluate that section in the text for him. (lifeissues.net)
My edited analysis of the section on "stem cell research" in this science textbook is copied below. (lifeissues.net)
In my opinion there is no question but that the scientific information on stem cell research included in this science text book being used in Illinois schools incorporates some inaccurate scientific facts, and seems to be very partial to the use of human embryonic "stem cell" research. (lifeissues.net)
What is cloning, and what does it have to do with stem cell research? (eurostemcell.org)
This form of cloning is unrelated to stem cell research. (eurostemcell.org)
This paper outlines the debates prompted through a reproduction mechanism involv- by progress in cloning research, with special ing male and female germ cells. (who.int)
In a Science "Policy Forum" related to the team's latest findings, David Magnus and Mildred Cho from Stanford University in Palo Alto, CA discuss international oversight and ethical issues in oocyte donation, including the need to promote realistic expectations of the outcomes of stem cell research. (scienceblog.com)
Other research groups such as Masako Tada's group in Japan in 2001 and Chad CowanÆs group in Massachusetts in 2005 combined embryonic stem cells with somatic cells to produce pluripotent cells. (asu.edu)
The 2009 Rosenstiel Award for Distinguished Work in Basic Medical Science will be awarded to three pioneers in stem cell research. (brandeis.edu)
Therapeutic cloning, which advocates claim holds the promise of one day helping to develop cures for diseases such as Alzheimer's and spinal cord injuries, is widely supported within the scientific research community, and has recently been given the imprimatur of the National Academy of Sciences. (commonwealmagazine.org)
Kass opposes all cloning, and there seems little chance that his commission, which is weighted heavily with thinkers who express similar skepticism about the direction and pace of biogenetic research, will issue a report approving therapeutic cloning. (commonwealmagazine.org)
The commission's likely refusal to embrace cloning despite the medical potential of stem-cell research has aroused the ire of many who are impatient with arguments about when life begins. (commonwealmagazine.org)
Those opposed to such research think that the logic of justification behind therapeutic cloning will set a dangerous precedent, legitimating experimentation on other human beings, born and unborn. (commonwealmagazine.org)
In particular, scientific developments in areas such as iPS cells open new possibilities of research and, at mid term, of therapeutic applications, but they also bring new ethical challenges and problems requiring further reflection and debate. (lifeissues.net)
Stem Cell Research? (jewishvaluesonline.org)
What is the Jewish perspective on stem cell research? (jewishvaluesonline.org)
If embryonic stem-cell research offers real possibilities for future cures then, from a Jewish point of view, it may be pursued with caution, humility, and strict supervision. (jewishvaluesonline.org)
In December 1999, the editors of Science, the journal devoted to scientific and medical matters, called stem cell research the 'Breakthrough of the Year. (cbc.ca)
Since then, there has been a flurry of announcements about developments in stem cell research and hints of promising treatments for diseases such as Alzheimer's, Parkinson's and cancer. (cbc.ca)
Ontario and California together account for about 70 per cent of the stem cell research currently conducted in North America. (cbc.ca)
Some of that money would be aimed at turning the state into the second-largest stem cell research region in the United States. (cbc.ca)
Michael Koch, a Ph.D. student within the BMLS research group said "As epithelial organoids have a stem cell-like character, which makes them difficult to transduce, they require optimised transduction protocols. (onenucleus.com)
For drug discovery research, AMSBIO offers assays, recombinant proteins and cell lines. (onenucleus.com)
5. In 2001, France and Germany requested the United Nations General Assembly to develop international conventions on human reproductive cloning, therapeutic cloning and research on stem cells. (who.int)

Before the technique will ever make it to the clinic, however, researchers must find a way to remove genomic material from the egg cell. (the-scientist.com)
The stem cells, the researchers showed, could turn into heart or nerve cells in the laboratory, and had other characteristics of established embryonic stem cell lines. (nih.gov)
The researchers stopped well short of creating a human clone. (nationalrighttolifenews.org)
The stem cells could be studied in the laboratory to help researchers understand what goes wrong in diseases like these. (eurostemcell.org)
The researchers generated these stem cell lines ten times more efficiently than in their 2004 Science study, using improved laboratory methods. (scienceblog.com)
The researchers added all of the twenty-four retroviral factors at the same time into mouse fibroblast cells. (asu.edu)
However, researchers at the Harvard Stem Cell Institute say reprogrammed cells won't eliminate the need or value of studying embryonic stem cells. (cbc.ca)
Using this procedure, called therapeutic cloning, the researchers succeeded in making cells from the tail of a mouse convert into the dopamine-producing cells of the brain that are lost in Parkinsons disease (figure 48.7). (biologywriter.com)
To investigate the underlying cell organization principles of organoids, BMLS researchers imaged hundreds of pancreas and cholangiocarcinoma organoids in parallel using microscopic techniques. (onenucleus.com)
As a result of this pioneering work - BMLS researchers were able to monitor development and quantify the behavior of organoids at the single-cell (microscale), individual-organoid (mesoscale), and entire-culture (macroscale) levels. (onenucleus.com)

Conceptual artist Jonathon Keats is giving everyone the chance to become the Christian messiah - and a number of other historical personages and celebrities as well - through his Epigenetic Cloning Agency, which opens a new branch at a Berlin gallery today (May 31). (livescience.com)
To be clear: The Epigenetic Cloning Agency is an art project whose concoctions are meant to inspire thought and conversation about the nature of identity and the march of scientific progress, not actually turn you into someone else. (livescience.com)
But epigenetic cloning takes a different tack, seeking to alter how a customer's genes are expressed rather than swapping out his or her entire genome. (livescience.com)
For example, the recipe for becoming Jesus Christ includes, among other things, some basic components of the Mediterranean diet of the time and substantial doses of omega-3 fatty acids (he likely ate a lot of fish), Keats told LiveScience back in October at the opening of the San Francisco branch of the Epigenetic Cloning Agency. (livescience.com)
The Epigenetic Cloning Agency's Berlin branch will be offering several other tinctures in addition to the Jesus mixture, including Napoleon Bonaparte, Queen Elizabeth I, Lady Gaga , swimmer Michael Phelps and Angela Merkel, the current chancellor of Germany. (livescience.com)
The Epigenetic Cloning Agency is willing to tailor tinctures to their customers' desires, and it will even take a stab at fictional characters such as Sherlock Holmes if asked to do so. (livescience.com)
Hochedlinger pushes cells' developmental rewind buttons to examine their epigenetic history. (rupress.org)
It was believed that the epigenetic signature and age-related changes such as shortened telomeres and oxidative DNA damage might hinder reprogramming of mature adult nuclei. (news-medical.net)

In fact, low levels of telomerase activity have been found in human adult stem cells including haematopoietic and non-haematopoietic stem cells such as neuronal, skin, intestinal crypt, mammary epithelial, pancreas, adrenal cortex, kidney, and mesenchymal stem cells (MSCs) ( Table 1 ). (nature.com)
This vector efficiently produced Tet-ON regulatable immortalized (293T) and primary (human mesenchymal stem cells and human primary fibroblasts) cells. (nih.gov)

The adult cell nuclei were transferred into metaphase-II stage human oocytes, producing a karyotypically normal diploid embryonic stem cell line from each of the adult male donor cells. (news-medical.net)

Cloning entails taking the nucleus - the compartment that contains the DNA - from an adult cell and putting it into an egg from which the original nucleus has been removed. (nih.gov)

antibody (clone AD2.35) on mouse primary astrocytes. (biolegend.com)
The cells were fixed, permeabilized, blocked and then stained with primary antibody, and Alexa Fluor® 594 conjugated secondary IgG (Cat. (biolegend.com)
The following product was used in this experiment: SOX2 (Embryonic Stem Cell Marker) Recombinant Rabbit Monoclonal Antibody (SOX2/4267R) from Thermo Fisher Scientific, catalog # 6657-RBM7-P1. (thermofisher.com)
Caco-2 cells were fixed, permeabilized, and labeled with Anti-Acetylated Alpha-Tubulin (Acetyl K40) Antibody, Clone TEU318 (Catalog #100-0753), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, iFluor™ 488. (stemcell.com)
Inset shows cells labeled with Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, iFluor™ 488 (with DAPI staining). (stemcell.com)
Applications Tested: This APB5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. (thermofisher.com)
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. (thermofisher.com)

While iPSCs avoid the ethical issues surrounding embryonic stem cells, the methods used to derive them sometimes induce mutations in cancer causing genes, making them unsuitable for therapeutic purposes. (the-scientist.com)
The field of induced pluripotent stem cells (iPSCs) has been around for 10 years. (addgene.org)
Retroviral vectors , such as pMXs or pMSCV, infect dividing cells, and have a higher efficiency of generating iPSCs compared to other delivery methods. (addgene.org)
These progenitors which are derived from either embryonic stem cells (ESCs) or healthy induced pluripotent stem cells (iPSCs) express wild-type levels of a-syn, thus making them equally susceptible to developing Lewy bodies over time. (lu.se)
The advent of iPSCs has opened up the possibility to graft patient-specific cells which most likely would circumvent the need for immunosuppression. (lu.se)

Their 'Human Cloning Prohibition Act of 2002' would prohibit human reproductive cloning by imposing significant criminal and civil penalties in the form of fines (at least $1 million) and up to ten years in prison. (boloji.com)

Thus, telomerase activity and telomere maintenance are associated with the immortality of cancer cells, germ-line cells, and embryonic stem (ES) cells. (nature.com)
Reproductive cloning versus germ cell (egg, ovum). (who.int)
Stem cells come in three forms: embryonic stem cells, embryonic germ cells and adult stem cells. (cbc.ca)

Detailed descriptions of methods used in animal cloning and biotechnology are provided in the report Animal Biotechnology: Science-Based Concerns (NRC, 2002). (nationalacademies.org)

Cell culture flasks used to propagate stem cells. (nih.gov)

Those were spindle removal, donor cell fusion, and cytoplast activation. (news-medical.net)

Keats is dedicating the Berlin office tonight with a reception that will serve a cocktail specially designed to create clones of himself. (livescience.com)

Canada should follow its lead and permit therapeutic cloning under strict regulation. (sentientdevelopments.com)
This spring the Senate will consider a bill sponsored by Tom Harkin (D-Iowa) that would ban cloning aimed at creating a child, but unlike the House bill, would permit therapeutic cloning. (commonwealmagazine.org)

These stem cells are encouraged to become the type of cells needed (say, insulin cells) and then introduced into the patient (with, for example, diabetes). (sentientdevelopments.com)
in other words, it has the potential to become any type of cell. (thefutureofthings.com)
Embryonic stem cells are immortal, and have the potential to develop into any type of adult cell, even after months growing in culture dishes. (news-medical.net)
A percentage is not listed because many variables (cell type, criteria used, etc.) can effect reprogramming rates and such a number maybe misleading. (addgene.org)
They are in an early stage of development and have the ability to become any type of cell to form skin, bones, organs or other body parts. (cbc.ca)
Embryonic stem cells are pluripotent - they have the ability to become virtually any type of cell within the body. (cbc.ca)
The new cells produce only about 2% as much insulin as normal cells do, so there is still plenty to learn, but the take-home message is clear: transplanted embryonic stem cells offer a path to cure type 1 diabetes. (biologywriter.com)
By using skin cells as the starting cell-type, he managed to identify the three molecules needed to "program" dendritic cells in just nine days. (lu.se)
Human T-cell leukemia virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) is a chronic neurodegenerative disease. (bvsalud.org)
Reactive leukocytosis can be classified on the basis of the white blood cell type affected. (medscape.com)

At just 31 years old, Hochedlinger has already worked on therapeutic cloning in a mouse model ( 2 ), reprogramming cancer nuclei ( 3 ), and the molecular mechanisms controlling stem cell pluripotency ( 4 ). (rupress.org)
Molecular cloning and expression of subunit 9 of the 26S proteasome.Hoffman L., Rechsteiner M.FEBS Lett. (joplink.net)
AMSBIO have supplied custom lentivectors to Francesco Pampaloni and his team at the Buchmann Institute for Molecular Life Sciences (BMLS) in Frankfurt, Germany allowing them to visualize cell nuclei and F-actin cytoskeleton in their organoids. (onenucleus.com)

Claims that you could clone individual treatments of human beings to treat common diseases like diabetes, suggests you need a huge supply of human eggs. (wikiquote.org)
Other improvements over the last paper include the reduced use of animal products in laboratory procedures and better evidence that the cell lines matched the patients' cells and did not have a parthenogenetic origin, where unfertilized eggs can divide on their own. (scienceblog.com)

Anatomy78

Organisms17

Diseases6

Chemicals and Drugs49

Analytical, Diagnostic and Therapeutic Techniques and Equipment46

Phenomena and Processes61

Disciplines and Occupations4

Technology, Industry, Agriculture1

Information Science3

Embryos33

Embryo36

Dolly17

Scientists26

Differentiation20

Blastocyst5

Genetic25

Tissues17

Genetically10

Reprogram6

Organism6

SCNT17

Somatic cell nucleus1

Involves7

Whose nucleus has been removed1

Debilitating diseases1

Mammalian cells2

Reproductive cloning of huma5

Similar to embryonic stem2

Pluripotent state4

19972

Therapies10

Potentially5

Nuclear Transfer11

Progenitor cells2

Successfully3

Therapeutic potential1

Humans5

Biology and Regenerative Medicine1

Organisms2

Bone marrow5

Tissue12

20231

Embryonic state2

Mice10

Differentiate12

Removed and replaced2

Research36

Researchers10

Epigenetic8

Mesenchymal stem2

Oocytes1

Adult cell and putting it int1

Antibody7

IPSCs5

Human reproductive1

Germ3

Biotechnology1

Propagate1

Donor cell1

Create clones1

Permit therapeutic2

Type10

Molecular3

Eggs2