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  • genes
  • In theory, the oocyte's cytoplasm would reprogram the transferred nucleus by silencing all the somatic cell genes and activating the embryonic ones. (sciencemag.org)
  • The egg was evidently able to reprogram the introduced nucleus and direct its genes to switch from the duties of an intestinal cell to those appropriate to a developing egg. (nytimes.com)
  • To reprogram the fibroblasts, the Hu lab used BRD3R delivered by a lentiviral vector, along with three of the genes that the Japanese Nobel Laureate Shinya Yamanaka used in his first successful reprogramming of mature mouse cells into iPSCs in 2006 - OCT4, SOX2 and KLF4. (uab.edu)
  • That would mean reactivating a whole lot of genes that skin cells don't need but eggs do. (scribd.com)
  • A potential use of stem cells genetically matched to a patient would be to create cell lines that have genes linked to a patient's particular disease. (wikipedia.org)
  • They form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency and can differentiate into endodermal, ectodermal and mesodermal cells both in vitro and in vivo. (wikipedia.org)
  • Brinster and longtime collaborator Richard Palmiter pioneered techniques to transfer foreign genes into mammals, and they utilized these methods to elucidate the activity and function of genes. (wikipedia.org)
  • The 3' UTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. (wikipedia.org)
  • That is, gene segments capable of producing antibodies are isolated and cloned into cells that can be grown in a bioreactor such that antibody proteins produced from the DNA of the cloned genes can be harvested en masse. (wikipedia.org)
  • nuclei
  • In case that does not work, though, the discussion has turned to the idea of transplanting adult nuclei into embryonic stem cells as a way of getting round the rejection problem. (scribd.com)
  • This nervousness has not been calmed by the activities of Advanced Cell Technology, a firm based in Worcester, Massachusetts, which announced in November 2001 that it had managed the trick of transplanting adult nuclei into stem cells and persuading the result to divide a few times. (scribd.com)
  • In January 2018, a team of scientists in Shanghai announced the successful cloning of two female crab-eating macaques (named Zhong Zhong and Hua Hua) from fetal nuclei. (wikipedia.org)
  • hESCs can be generated by SCNT using dermal fibroblasts nuclei from both a middle-aged 35-year-old male and an elderly, 75-year-old male, suggesting that age-associated changes are not necessarily an impediment to SCNT-based nuclear reprogramming of human cells. (wikipedia.org)
  • The first mouse born was named Cumulina, after the cumulus cells whose nuclei were used to clone her. (wikipedia.org)
  • mammalian cells
  • Abgent has over fifteen years of experience producing recombinant proteins in E. coli and mammalian cells (CHO and HEK293, etc), and we have added a powerful yeast expression platform to our menu of services. (abgent.com)
  • Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. (wikipedia.org)
  • As discussed above, this is achieved through recombinant DNA methods using an appropriate vector and expression in mammalian cells. (wikipedia.org)
  • transplantation
  • Gurdon's recent research has focused on analyzing inter cellular signal ling factors involved in cell differentiation, and on elucidating the mechanisms involved in reprogramming the nucleus in transplantation experiments, including demethylation of the transplanted DNA. (blogspot.com)
  • Somatic cell nuclear transplantation has become a focus of study in stem cell research. (wikipedia.org)
  • vitro
  • The production of Megan and Morag demonstrated that viable sheep can be produced by nuclear transfer from cells which have been cultured in vitro. (wikipedia.org)
  • Trounson's areas of interest include cloning, stem cells, biotechnology, cloning for agricultural industry, gene storage and in-vitro fertilisation. (wikipedia.org)
  • This capacity to continue to regenerate new cells has been shown in in vitro conditions for some animals in which all other cells have died, especially if the animal died from exposure to cold elements. (wikipedia.org)
  • regenerative medicine
  • Trounson was the President of the California Institute for Regenerative Medicine between 2007 and 2014, a former Professor of Stem Cell Sciences and the Director of the Monash Immunology and Stem Cell Laboratories at Monash University, and retains the title of Emeritus Professor. (wikipedia.org)
  • tissues
  • Our finding offers new ways of generating stem cells for patients with dysfunctional or damaged tissues and organs. (medicalnewstoday.com)
  • Such stem cells can regenerate and replace those damaged cells and tissues and alleviate diseases that affect millions of people. (medicalnewstoday.com)
  • Crown antibodies pass additional stringent quality requirements, including extended control sets, uniform results against multiple biologically relevant cell lines and tissues, and function in multiple applications. (abgent.com)
  • Biologists hope the technique will enable replacement tissues to be generated from a patient's own cells for use against a wide variety of degenerative diseases. (nytimes.com)
  • The stem cell theory of aging postulates that the aging process is the result of the inability of various types of stem cells to continue to replenish the tissues of an organism with functional differentiated cells capable of maintaining that tissue's (or organ's) original function. (wikipedia.org)
  • There are also several challenges when it comes to therapeutic use of stem cells and their ability to replenish organs and tissues. (wikipedia.org)
  • believed that these unique cells lie dormant until activated by injury or disease, and that they have the potential to regenerate tissues lost to disease or damage. (wikipedia.org)
  • reproductive
  • One of the headaches for the advocates of NT-hESC is that potentially each advance in making NT-hESC (therapeutic cloning) could unintentionally also make it easier for some crazy folks to try to actually clone a person (reproductive cloning or "Star Wars" type cloning). (ipscell.com)
  • Think that reproductive human cloning is impossible? (ipscell.com)
  • Further, even failed attempts at human reproductive cloning (and it's very likely the first attempts at human repro cloning would be horrible failures potentially producing deformed or dead humans) could unfairly, but rather quickly sink therapeutic cloning. (ipscell.com)
  • I personally do not believe that there is any insurmountable technical obstacle to human reproductive cloning as it has worked for many mammals in the past and animal cloning is more common now than ever. (ipscell.com)
  • Above is a picture from my book, Stem Cells: An Insider's Guide explaining the differences between reproductive and therapeutic human cloning. (ipscell.com)
  • Gábor Vajta publications indexed by Google Scholar "Website of Reproductive Biomedicine Online (journal)" "Website of Cryobiology (journal)" "Website of Reproduction, Nutrition, Development (journal)" "Website of Theriogenology (journal)" "Website of Animal Reproduction Science (journal)" "Website of Cellular Reprogramming (journal)" "Cell Nuclear Transfer" patent no. (wikipedia.org)
  • germ
  • Normally a female germ cell begins to divide when it has about 150 cells - at this point stem cells can be harvested. (medicalnewstoday.com)
  • In recent years, Brinster has continued to advance the field of stem cell biology, having made a series of catalyzing, transformational discoveries utilizing male germ line stem cells. (wikipedia.org)
  • with Mario Capecchi and Oliver Smithies 2003 Selected for the Hall of Honor National Institute of Child Health and Human Development (15 members, total) 2006 Gairdner Foundation International Award, Canada "for pioneering discoveries in germ line modification in mammals. (wikipedia.org)
  • research
  • Let's consider two cases: human cloning and stem cell research. (apologeticspress.org)
  • Another topic that is often discussed on the news is stem cell research. (apologeticspress.org)
  • Research scientists from Bristol University have received funding from Catholic parishioners, to use adult stem cells for therapeutic applications, reports the BBC. (bionews.org.uk)
  • Now, a team at the Research Institute for Stem Cell Research at CHA Health Systems in Los Angeles and the University of Seoul said they have achieved the same result with two adult males, 'The Telegraph' reported. (stem-cells-news.com)
  • So if you can't do this with adult cells it is of limited value," said Robert Lanza , co-author of the research published in the journal Cell Stem Cell . (stem-cells-news.com)
  • In November 2007, two research groups, one at the University of Wisconsin-Madison and another at Kyoto University in Japan, announced that they had succeeded in directly reprogramming human skin cells into stem cells. (opposingviews.com)
  • Yesterday, as many as 700 new stem cell lines were approved for use in federally funded research by the National Institutes of Health, reversing the policy of the George W. Bush administration to restrict funding to just a handful of approved cell lines on ethical grounds. (opposingviews.com)
  • But some countries are getting nervous about stem-cell research. (scribd.com)
  • Alan Osborne Trounson (born 16 February 1946) is an Australian embryologist with expertise in stem cell research. (wikipedia.org)
  • He was a professor of theriogenology and biotechnology at Seoul National University (dismissed on March 20, 2006) who became infamous for fabricating a series of experiments, which appeared in high-profile journals, in the field of stem cell research. (wikipedia.org)
  • On May 12, 2006, Hwang was charged with embezzlement and bioethics law violations after it emerged much of his stem cell research had been faked. (wikipedia.org)
  • The Korea Times reported on June 10, 2007, that Seoul National University fired him, and the South Korean government canceled his financial support and barred him from engaging in stem cell research. (wikipedia.org)
  • In February 2011, Hwang visited Libya as part of a $133 million project in the North African country to build a stem cell research center and transfer relevant technology. (wikipedia.org)
  • His alleged success was touted as the fifth instance in the world in cow cloning, with a notable caveat: Hwang failed to provide scientifically verifiable data for the research, giving only media sessions and photo-ops. (wikipedia.org)
  • tadpoles
  • Dr. Gurdon's discovery came in 1962, when he produced living tadpoles from the adult cells of a frog. (nytimes.com)
  • Gurdon began cloning experiments using nonembryonic cells-specifically, cells from the intestinal lining of tadpoles. (blogspot.com)
  • Gurdon believed that the tadpoles were old enough so that cells taken from them would be differentiated. (blogspot.com)
  • But the tadpoles cloned in Gurdon's early experiments never survived to adulthood and scientists now believe that many of the cells used in these experiments may not have been differentiated cells after all. (blogspot.com)
  • Wilmut
  • However, in 2008 Wilmut announced that he would abandon the technique of somatic cell nuclear transfer by which Dolly was created in favour of an alternative technique developed by Shinya Yamanaka. (wikipedia.org)
  • world's
  • On November 2015, a Chinese biotech company Boyalife Group announced that it will partner with Hwang's laboratory, Sooam Biotech, to open the world's largest animal cloning factory in Tianjin as early as 2016. (wikipedia.org)
  • 1962
  • 1962, John Gurdon discovers the specialization of cells is reversible. (uab.edu)
  • Alzheimer's
  • Researchers are trying to use these special cells to cure illnesses such as Alzheimer's disease. (apologeticspress.org)
  • Diseases such as Alzheimer's disease, end-stage renal failure and heart disease are caused by different mechanisms that are not related to stem cells. (wikipedia.org)
  • researchers
  • However, because iPS can sometimes cause unexpected mutations in the cells, researchers have been seeking alternative methods. (medicalnewstoday.com)
  • In order to make Dolly, researchers took a cell from a six year old and starved that cell almost to death. (apologeticspress.org)
  • Now some of the researchers want to clone humans. (apologeticspress.org)
  • In a paper published March 7 in Nature Communications, Hu and fellow UAB researchers describe their successful hunt for a reprogramming factor that boosts the efficiency and shortens the time the cell takes to reprogram. (uab.edu)
  • Last year, a team of researchers had created stem cells from the skin cells of babies but it was unclear whether it would work in adults because cells mutate with age. (stem-cells-news.com)
  • genome
  • Because the cell-size of less than 5 micrometers seems rather small as to contain the entire human genome the authors speculate on the "concept of a minimal genome"[citation needed] for these cells. (wikipedia.org)
  • organism
  • Spore-like cells were said to be a specific class of stem cells in adult organisms, including humans, which are small, versatile, and most frequently remain in a dormant "spore-like" state as the rest of the cells of the organism divide, grow, and die. (wikipedia.org)
  • antibody
  • Chimeric antibody names contain a -xi- stem. (wikipedia.org)
  • That is, after an antibody is developed to have the desired properties in a mouse (or other non-human), the DNA coding for that antibody can be isolated, cloned into a vector and sequenced. (wikipedia.org)
  • animal
  • Previously, human ES cells could only be cultivated using other cells or animal substances. (bionews.org.uk)
  • In 1924 Spemann and Mangold demonstrated the key importance of cell-cell inductions during animal development. (wikipedia.org)
  • As of 2005[update], Megan was still alive and was the oldest cloned animal at the time. (wikipedia.org)
  • So-called "testis" in an adult animal is a receptacle of cells from larval males. (wikipedia.org)
  • Although Hwang had already established himself as an expert in animal cloning and secured celebrity status in South Korea in the late 90s, his alleged sudden success came as a surprise because this was the first reported success in human somatic cell cloning. (wikipedia.org)
  • accumulates
  • After division svp accumulates in both daughter cells and down-regulates Hb. (wikipedia.org)
  • Aging of the hair follicle appears to be primed by a sustained cellular response to the DNA damage that accumulates in renewing stem cells during aging. (wikipedia.org)