Stem cell gene expression. Medical search. Frequent questions

Efficient commitment to functional CD34+ progenitor cells from human bone marrow mesenchymal stem-cell-derived induced pluripotent stem cells. (1library.org)
Hart, D.A. (2014) Why Mesenchymal Stem Cell/Progenitor Cell Heterogeneity in Specific Environments? (scirp.org)
Wu, Y. and Zhao, R.C. (2012) The Role of Chemokines in Mesenchymal Stem Cell Homing to the Myocardium. (scirp.org)
Li, L. and Jiang, J. (2011) Regulatory Factors of Mesenchymal Stem Cell Migration into Injured Tissues and Their Signal Transduction Mechanisms. (scirp.org)
Fehrer, C. and Lepperdinger, G. (2005) Mesenchymal Stem Cell Aging. (scirp.org)

Top PDF Morphologic and gene expression criteria for identifying human induced pluripotent stem cells. (1library.org)
Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells. (1library.org)
DNA methylation dynamics in human induced pluripotent stem cells over time. (1library.org)
Human induced pluripotent stem cells differentiation into oligodendrocyte progenitors and transplantation in a rat model of optic chiasm demyelination. (1library.org)
Persistent donor cell gene expression among human induced pluripotent stem cells contributes to differences with human embryonic stem cells. (1library.org)
Negligible immunogenicity of induced pluripotent stem cells derived from human skin fibroblasts. (1library.org)
Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression. (1library.org)
Genetic background drives transcriptional variation in human induced pluripotent stem cells. (1library.org)
Scalable passaging of adherent human pluripotent stem cells. (1library.org)
By using appropriate promoter combinations and shielding the LVs with the Is2 insulator, we have constructed the Lent-On-Plus Tet-On system that achieved efficient transgene regulation in human multipotent and pluripotent stem cells. (nih.gov)
To our knowledge, Lent-On-Plus is the first all-in-one vector system that tightly regulates transgene expression in bulk populations of human pluripotent stem cells and its progeny. (nih.gov)
Melinda is highly proficient in the use of in vitro and in vivo assays for normal and malignant hematopoietic stem and progenitor cells, determination of self-renewal, expansion and lineage specific differentiation capabilities, and the use of induced pluripotent stem cells for the investigation of hematopoietic disorders and leukemic progression and the testing of targeted therapeutics. (edu.au)
Firstly, we present a robust 2-week protocol for the differentiation of human pluripotent stem cells (PSCs) into forebrain neural progenitor cells. (lu.se)

germline stem cell development illuminate the biology of highly potent stem cells. (bvsalud.org)
Chromatin and gene expression changes during female Drosophila germline stem cell development illuminate the biology of highly potent stem cells. (bvsalud.org)
The findings advance fundamental knowledge of how germline stem cells become egg cells , and could lead to important findings in developmental biology . (bvsalud.org)
The interplay between stem cell and tumor biology offers an exceptional opportunity to advance our knowledge about cancer, one of the leading causes of death worldwide, with potential impacts in cancer diagnosis and therapy. (hindawi.com)
Based on presentations by world-renowned investigators at the 73rd annual Cold Spring Harbor Symposium on Quantitative Biology, this volume reviews the latest advances in research on the control and regulation of stem cells. (cshlpress.com)
Research in the Paralkar Lab spans the spectrum from human patient sample studies and mouse models to cutting-edge molecular biology tools, high-throughput sequencing approaches, and novel computational algorithms, all with the goal of gaining insight into how the transcription of coding genes and noncoding ribosomal DNA genes is regulated in hematopoietic stem cells, myeloid progenitors, and in leukemia. (upenn.edu)
Single-cell level characterization of embryonic development is a major benchmark of human developmental biology. (newswise.com)
He serves on the Editorial Boards of Annual Review of Cell and Developmental Biology and Genome Biology. (elifesciences.org)
She is currently a Senior Investigator leading the Chromosome Structure and Epigenetics Mechanism Unit within the Laboratory of Receptor Biology and Gene Expression at the Center for Cancer Research of the NCI/NIH in Bethesda. (elifesciences.org)
Using a combination of chromatin biochemistry, computational modelling, atomic force microscopy (AFM), genetics, genomics and cell biology, Dr. Dalal and colleagues are investigating whether chromatin adopts alternate structural conformations in cancer cells, the functional consequences of large-scale chromosomal alterations upon the cancer epigenome, and identifying small molecules which can target these structures or processes. (elifesciences.org)
Led by Sui Huang, MD, PhD, a Visiting Associate Professor in the Children's Hospital Boston Vascular Biology Program (now also on the faculty of the University of Calgary), and Hannah Chang, an MD/PhD student in Children's Vascular Biology Program, the researchers examined how blood stem cells "decide" to become white blood cell progenitors or red blood cell progenitors. (sciencedaily.com)
Development: For advances in developmental biology and stem cells. (lu.se)

This balance is maintained by the combinatorial activity of transcription factors and chromatin proteins that dictate the transcription of coding gene networks instructing fate choice decisions. (upenn.edu)
Similarly, while your genes are "fixed", the expression of those genes - the amount of proteins they cause to be made, whether or not they are even switched on or off at all - depends on the "environment," the circumstances surrounding those genes . (marksdailyapple.com)
However, new research shows that the decision is made collectively by an entire network of genes and proteins. (sciencedaily.com)
Huang and Chang then looked at the proteins GATA1 and PU.1, transcription factors that normally favor differentiation into red and white blood cells, respectively. (sciencedaily.com)
We have learned that genes in mammalian cells are transcribed into messenger RNAs (mRNAs), which are to be translated into polypeptides (proteins). (intechopen.com)
This process is mediated by a complex molecular machinery known as the spliceosome, which enables the production of multiple and functionally distinct proteins from single genes. (lu.se)
[ 8 ] In the studies in which HO was induced experimentally, 2 factors were found to be prerequisites for ectopic ossification: (1) traumatic ischemic degeneration of involved muscle and (2) tissue expression of bone morphogenic proteins (BMPs). (medscape.com)

Human colonic epithelial cell renewal, proliferation, and differentiation are stringently controlled by numerous regulatory pathways. (utmb.edu)
The TAL1/SCL transcription factor regulates cell cycle progression and proliferation in differentiating murine bone marrow monocyte precursors. (nih.gov)
The rate of ribosome production has far-reaching influence on the fate of the cell, and dictates its size, proliferation, and ability to translate global or specific mRNAs. (upenn.edu)
Gene expression must be appropriately maintained to regulate development, differentiation, and proliferation of cells. (intechopen.com)
B) Analysis of stem cell proliferation marker gene LGR5 expression by quantitative reverse transcription PCR in undifferentiated HIEs. (cdc.gov)
ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)

In addition to retinal cells, other stem-cell derivatives are likely to play an important role in the future of regenerative medicine. (news-medical.net)
The researchers of the paper from Advanced Cell Technology, collaborated with scientists from the Institute of Regenerative Medicine at Wake Forest University School of Medicine, Winston-Salem, North Carolina, and the University of Chicago, Chicago, Illinios. (news-medical.net)
Advanced Cell Technology is a leading biotechnology company in the emerging field of regenerative medicine. (news-medical.net)
Overall, Wnt-5a is a new corneal epithelial wound healing stimulator that can be targeted to improve wound healing and stem cells in the diabetic cornea," write Ruchi Shah, PhD, of the Board of Governors Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, California, and colleagues. (medscape.com)

Furthermore, our data analysis revealed that the cell cycle, urea cycle, p53 signaling, and metabolism of amino groups are significantly implicated in the regulation of hESC differentiation. (molcells.org)
The generation of inducible stem cell lines with the Lent-ON-Plus LVs did not require selection or cloning, and transgene regulation was maintained after long-term cultured and upon differentiation toward different lineages. (nih.gov)
In this paper we summarise recent advances in our knowledge of the regulation of Xist and Tsix in embryonic stem (ES) and differentiating ES cells. (hal.science)
We found that both MSC and OEC transplanted acutely after injury induce an early up-regulation of genes related to tissue protection and regeneration. (uab.cat)
In early 2008, the National Institutes of Health established an epigenomics program to coordinate such efforts to better understand how this method of gene regulation fits into normal development, aging, learning and memory as well as its role in cancer, obesity, depression and other disease. (sciencedaily.com)
The topics covered include nuclear reprogramming, regulation of stem cell self-renewal and differentiation, the stem cell niche, and signaling and gene regulation in stem cells. (cshlpress.com)
The Paralkar Lab has identified that key hematopoietic and leukemic transcription factors bind to rDNA and regulate rRNA transcription, and we are interested in understanding how the binding of cell-type-specific transcription factors regulates the activity of Polymerase I and the transcription of rRNA in normal hematopoiesis, and how this regulation is co-opted in leukemia to drive abundant ribosome biogenesis. (upenn.edu)
RNA splicing is a major nexus of gene expression regulation, shaping cellular identity during development, frequently altered in human cancers. (lu.se)

abstract = "Transplantation of bone marrow derived mesenchymal stromal cells (MSC) or olfactory ensheathing cells (OEC) have demonstrated beneficial effects after spinal cord injury (SCI), providing tissue protection and improving the functional recovery. (uab.cat)
However, the changes induced by these cells after their transplantation into the injured spinal cord remain largely unknown. (uab.cat)
These data suggest a regulatory effect of MSC and OEC transplantation after SCI regarding tissue repair processes, but a fast rejection response to the grafted cells. (uab.cat)
Torres-Espín, A , Hernández, J & Navarro, X 2013, ' Gene Expression Changes in the Injured Spinal Cord Following Transplantation of Mesenchymal Stem Cells or Olfactory Ensheathing Cells ', PLoS ONE , vol. 8, e76141. (uab.cat)
Cytomegalovirus reactivation is still a critical concern following allogeneic hematopoietic cell transplantation, and cellular immune reconstitution of cytomegalovirus-specific cytotoxic T-cells is necessary for the long-term control of cytomegalovirus reactivation after allogeneic hematopoietic cell transplantation. (nature.com)
These methods and results can help us to better understand immune reconstitution following hematopoietic cell transplantation, leading to future studies on the clinical application of adoptive T-cell therapies. (nature.com)
This study describes for the first time, a differentiation system that does not require co-culture with animal cells or products, thus allowing the production of pathogen-free RPE cells suitable for transplantation into patients. (news-medical.net)
The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
About 675 publications in the fields of neuroanatomy, neuronal regeneration, cell transplantation and repair in the central nervous system. (lu.se)

In our study, we worked on cells derived from the CD34 + hematopoietic stem and progenitor cells, enriched with CD133 + population, and used culture media promoting growth of hematopoietic progenitors. (hindawi.com)
Blood stem cells with low levels of Sca-1 differentiated into red blood cell progenitors seven times more often than cells high in Sca-1 when exposed to erythropoietin, a growth factor that promotes red blood cell production. (sciencedaily.com)

ALC-2 expression has proven to be a useful marker of cardiac muscle chamber distinction, development and differentiation. (wikipedia.org)
EPCs are enriched in the population coexpressing CD34, CD133, and KDR with a low or absent expression of pan-hematopoietic marker CD45 [ 1 ]. (hindawi.com)
Further, when those MSCs were analyzed for their specific marker expression using flowcytometry, they were found to be positive for CD29, CD73, CD90, and CD105 and negative for CD34 and CD45 stem cell markers respectively. (journalcra.com)
To identify genetic programs of human colonic epithelial cell differentiation in vivo as well as candidate marker genes that define colonic epithelial stem/progenitor cells and the stem cell niche, we applied gene expression analysis of normal human colon tops and basal crypts by using expression microarrays with 30,000 genes. (utmb.edu)
They began by examining populations of seemingly identical blood stem cells, and found that a cell marker of "stemness," a protein called Sca-1, was actually present in highly variable amounts from cell to cell -- in fact, they found a 1,000-fold range. (sciencedaily.com)
RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). (bvsalud.org)
Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. (bvsalud.org)
The finding of Wnt-5a as a new diabetic corneal marker regulating wound healing and stem cell function may have implications for other diabetes complications involving impaired wound healing, including diabetic foot ulcers, as they share similar neurovascular, sensory, and immunological compromise with diabetic eye disease, Shah and colleagues say. (medscape.com)
The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)

Taken together, the data indicates that gene topology causes NSD1 to deposit H3K36me2 throughout the genome and represses transcription. (epigenie.com)
Transposable elements - fragments of DNA that can copy themselves and integrate into different parts of the genome - can greatly disrupt the integrity of the germ cell genome . (bvsalud.org)
Systems involving small RNAs and DNA methylation , which respectively modify the sequence and structure of the genome , can protect germ cells from the activity of transposable elements . (bvsalud.org)
Current bioinformatic pipelines for high-throughput studies like whole genome sequencing, RNA-seq, ChIP-seq, and single cell RNA-seq are limited in their ability to map repetitive elements of the genome like ribosomal DNA. (upenn.edu)
In a final step, Huang and Chang used microarrays to look at the cells' entire genome. (sciencedaily.com)

rRNA transcription rates vary dramatically between different normal cell types in the hematopoietic tree, and leukemic cells have characteristic prominent nucleoli, indicating robust ribosome synthesis. (upenn.edu)
The thesis work would focus on identification of genes that are essential for the optimal functioning of the ubiquitin-proteasomal system in leukemic cells. (lu.se)

The complex epigenetic process leading to X-inactivation is largely controlled by Xist and Tsix, two non-coding genes of opposing function. (hal.science)
The Paralkar Lab has developed customized genomes and computational pipelines to map datasets to rDNA, and we are interested in developing advanced tools to map and interpret the genetic and epigenetic profiles of rDNA in normal and malignant cells. (upenn.edu)
Research in gene expression is exploding right now and is examining both the impact of environmental factors and the promise of epigenetic therapies. (marksdailyapple.com)
The discovery does not affect gene sequence but entails specific DNA modifications altering gene expression - what are known as epigenetic alterations," he said. (medscape.com)

The establishment of large-scale genomic collaborative efforts along with the development of new single-cell transcriptomic techniques and multi-omics approaches have enabled characterization of the mutational and transcriptional profiles of many cancer types and helped to identify clinically actionable alterations as well as predictive and prognostic biomarkers. (cdc.gov)

From the abstract: 'Neoadjuvant or adjuvant immunotherapy can improve outcomes in patients with resectable non-small-cell lung cancer (NSCLC). (cdc.gov)

By comparing their expression patterns, we determined novel hESC-specific genes and transcriptomes that could serve as reliable hESC markers associated with the "stemness" phenotype. (molcells.org)
Resveratrol showed inhibitory effects on cell behaviour related to the development of endometriosis by differentially affecting growth, apoptosis, migration and stem cell phenotype of endometrial and endometriotic cells in vitro. (nih.gov)
However, the features initially ascribed to endothelial progenitor cells, i.e ., phenotype, expression of endothelial markers, and ability to form tube-like structures on Matrigel, can be mimicked by blood monocytes [ 3 ]. (hindawi.com)
Adaptation to hypoxia and consequent pro-inflammatory gene expression of prostate and breast carcinomas have been implicated in the progression toward cancer malignant phenotype. (biomedcentral.com)
The aim of our study was to analyze the hypoxic and pro-inflammatory microenvironment in GBMs and to demonstrate that in a stem/progenitor cell line derived from human glioblastoma (GBM-SCs), hypoxia activates a coordinated inflammatory response, evidencing an invasive and migratory phenotype. (biomedcentral.com)
Advances in this field point to a more complex model of tumor development than previously envisioned, in which the stem-like phenotype may be dynamically acquired by cancer cells. (hindawi.com)
Ando, W., Heard, B., Nakamura, N., Frank, C.B. and Hart, D.A. (2012) Ovine Synovial Membrane-Derived Mescenchymal Progenitor Cells Retain the Phenotype of the Original Tissue That Was Exposed to in Vivo Inflammation: Evidence for a Suppressed Chondrogenic Differentiation Potential of the Cells. (scirp.org)

MSCs can be differentiated along different cell lineages of mesodermal origin including osteoblasts, chondrocytes, skeletal myocytes or visceral stromal cells 9 . (nature.com)
In-vitro cultures of primary endometriotic stromal cells, immortalized endometrial stromal (St-T1b) and endometriotic epithelial (12Z) cells were used to assess the effects of resveratrol on endometrial cell mechanisms. (nih.gov)
In the 1980's, Arnold Caplan and his colleagues published an isolation method of fibroblast-like stromal cells from bone marrow and first identified them as mesenchymal stem cells (MSCs) because of their multilineage differentiation potential ( Caplan, 1991 ). (frontiersin.org)

Again, they found tremendous variability within the apparently uniform cell population: more than 3,900 genes were differentially expressed (turned "on" or "off") between the low- and high-Sca-1 cells. (sciencedaily.com)

The establishment of the first human embryonic stem cells (hESCs) in 1998 provided a unique tool for studying human development. (molcells.org)
In this context, we conducted microarray-based differential gene expression analyses using two Korean embryo-derived hESC lines (CHA3 and CHA4) to identify undifferentiated and spontaneously dif-ferentiated (human embryoid body, or hEB) status. (molcells.org)
Evaluation of the cardiotoxicity of mitragynine and its analogues using human induced pluripotent stem cell-derived cardiomyocytes. (1library.org)
Expression of ALC-2 has been shown to correlate with expression of alpha-myosin heavy chain in cardiac atria of non-human primates. (wikipedia.org)
Conditional transgene expression in human stem cells has been difficult to achieve due to the low efficiency of existing delivery methods, the strong silencing of the transgenes and the toxicity of the regulators. (nih.gov)
In the present study, we aim the generation of Tet-On all-in-one lentiviral vectors (LVs) that tightly regulate transgene expression in human stem cells using the original TetR repressor. (nih.gov)
Conditioned media from such cells were then used to stimulate human aortic endothelial cells (HAoECs) to enhance tube-like structure formation in a Matrigel assay. (hindawi.com)
Concluding, high concentrations of atorvastatin stabilize tubes and enhance the paracrine angiogenic activity of human PAC cells in vitro . (hindawi.com)
Adult human bone marrow (BM) is the major source of mesenchymal stem cells (MSC) for cell-based therapies. (journalcra.com)
And hence, this study suggests that human bone marrow is a potential source of mesenchymal stem cells that could be differentiated in to ccardiomyocytes and used for cell based therapies for treating various cardiovascular diseases. (journalcra.com)
Collectively, the expression data set provides a comprehensive picture of human colonic epithelial cell differentiation. (utmb.edu)
In human solid glioblastoma we have observed a coordinated overexpression of a panel of pro-inflammatory genes as compared to host normal tissue. (biomedcentral.com)
Over the past decade, the identification of stem-like cells as drivers of tumor initiation, recurrence, and metastasis spread in a variety of human cancers had a tremendous impact in basic and translational cancer research. (hindawi.com)
Human ortholog(s) of this gene implicated in acute lymphoblastic leukemia. (nih.gov)
Gimble, J.M., Bunnell, B.A. and Guilak, F. (2012) Human Adipose-Derived Cells: An Update on the Transition to Clinical Translation. (scirp.org)
We differentiated several human embryonic stem cell lines into RPE," said Irina Klimanskaya, Senior Scientist at ACT, and first author of the study. (news-medical.net)
Human embryonic stem cells are so exciting because they provide a pathway of making many desperately-needed cell types for use in medicine," said Michael D. West, President & C.E.O. at ACT and an author on the paper. (news-medical.net)
Besides use in the aging retina, human embryonic stem cells have now demonstrated the potential to change the face of several age-related degenerative diseases, perhaps one day allowing physicians to prescribe young cells they way they dispense pills today. (news-medical.net)
Here, we defined the single-cell spatiotemporal gene expression landscape of human embryonic development with stem-cell-derived organoids. (newswise.com)
We established the human embryonic organoid (HEMO) from expanded potential stem cells and achieved both embryonic and extraembryonic tissues in the same organoid. (newswise.com)
Time-series single-cell RNA sequencing paired with single-cell resolution spatial revealed human embryonic development signatures such as extraembryonic placenta, yolk sac hematopoiesis neural crest, blood vessels, and cardiac mesoderm. (newswise.com)
Overall, our study advances the spatiotemporal analysis of human embryonic development in stem-cell-derived organoids. (newswise.com)
STEMdiff™ Neural Progenitor Medium is a defined and serum-free medium for the expansion of neural progenitor cells (NPCs) derived from human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium (Catalog #05835). (stemcell.com)
Recent global analyses of gene transcripts revealed that specific transcription factors (TFs) and their networking systems physiologically correspond to the onset of human diseases, including cancer. (intechopen.com)
We show for the first time that single-walled CNT and to a lesser extent multi-walled and its COOH-functionalized form induced CAF-like cells, which are non-tumorigenic in animals, but promote tumor growth of human lung carcinoma and CNT-transformed lung epithelial cells. (cdc.gov)
Changes occur in all cells of the human body as we age. (lu.se)
Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). (bvsalud.org)
Possibility to work with primary human hematopoietic stem cells. (lu.se)
A team of researchers led by Dr. Cristian Bellodi recently discovered a hardwired genetic control mechanism modulating individual spliceosomal components, known as splicing factors, in cells harboring oncogenic lesions common in human cancers. (lu.se)
To circumvent these obstacles, we have developed two methods for the investigation of human neural cells in culture. (lu.se)
We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)
2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)

The effects of resveratrol on 12Z and St-T1b cell viability were assessed by MTT assay, apoptosis by FITC Annexin V assay and cleaved caspase-3 levels and cell migration by wound healing assay. (nih.gov)
Elimination of BCR/ABL-dependent intracellular signals triggers apoptosis, but it is unclear whether this activates additional cell survival and/or death pathways. (lu.se)
Pathway analysis revealed the differential expression of genes involved in cell cycle maintenance and apoptosis, as well as genes in bone morphogenetic protein (BMP), Notch, Wnt, EPH, and MYC signaling pathways. (utmb.edu)
Increasing tumor size requires that GBM tumor cells maintain a balance between adaptation to hypoxia and cell death (apoptosis and central necrosis) through activation of hypoxia-inducible transcription factor 1 (HIF-1). (biomedcentral.com)

The effect of resveratrol on the expression of genes related to cell migration, angiogenesis and cell stemness was evaluated by qRT-PCR. (nih.gov)

The mouse blastocyst-derived embryonic stem cell (ES cell) line BLC6 efficiently differentiates into myosin heavy chain-, desmin- and myogenin- positive skeletal muscle cells when cultivated in embryo-like aggregates (embryoid bodies). (uni-luebeck.de)
In the ongoing debate over embryonic stem cells some have argued that the cells have not demonstrated their potential value in medicine. (news-medical.net)
Studies of embryonic stem cells and adult stem cells are covered, along with research shedding light on the roles of these cells in regeneration and cancer. (cshlpress.com)
Moreover, targets of the polycomb repressor complex in breast cancer and embryonic stem cells were more methylated in luminal B tumours than in other tumours. (lu.se)

Further experiments investigated how these changes impact the transition from stem cell to egg cell , revealing that germline stem cells express a wide diversity of genes , including most genes whose transcripts will be stored in the mature egg later on. (bvsalud.org)
In situ hybridization demonstrates transcripts for myogenin and myoD accumulating in mono- and multinucleated myogenic cells, while myf5 mRNA is already found in mononucleated myoblasts. (uni-luebeck.de)
Lund University researchers find that individual blood stem cells (depicted as snowflakes in this image) express stress-related transcripts when subjected to experimental procedures involving cell incubation at elevated temperatures. (lu.se)

Rat stem-cell leukemia gene expression increased during testis maturation. (nih.gov)
Cell-cell communication network analysis demonstrated that trophoblast-like tissues supplied WNT signaling in neural crest cells to facilitate maturation and migration. (newswise.com)

The gene profiles were analyzed by clustering and functional enrichment analysis based on the Gene Ontology database. (uab.cat)
The myocytes also express functional nicotinic cholinoceptors and exhibit T-type Ca 2+ currents and later L-type Ca 2+ currents, demonstrating physiological properties of skeletal muscle cells. (uni-luebeck.de)
Gene knockdown experiments showed that an expression of podoplanin on CAF-like cells is essential for their effects, indicating the functional role of CAF-like cells and podoplanin in CNT tumorigenic process. (cdc.gov)

DNMT3A is putting down non-CG methylation-mostly at CA (mCA)-and MeCP2 reads these marks to regulate enhancers and genes. (epigenie.com)
In contrast, cells transplanted at 7 days after injury down-regulate genes related to tissue regeneration. (uab.cat)
Dr. Robertson, who also works in breast cancer and brain tumors as well as colon cancer, is finding patterns of genes that regulate healthy cell differentiation are shut off in cancers. (sciencedaily.com)
When you change to a diet low in sugars and rich in healthy fats, those or other genes are directed to reduce inflammatory expression, down-regulate insulin-producing metabolic machinery, up-regulate insulin receptors and rebuild cell membranes to reflect the presence of better building materials (omega 3 fatty acids, etc. (marksdailyapple.com)

We have developed a computational modeling framework for studying this process, where the combined effects of mechanical and genetic interactions are analyzed within the context of proliferating cells. (lu.se)
To our knowledge, this is the first attempt to combine cell-based spatial mechanical simulations with genetic networks to explain mammalian embryogenesis. (lu.se)
According to a recent study by researchers from the Lund Stem Cell Center at Lund University, published in Nature Aging, the genetic changes previously observed in aging blood stem cells could be linked to cell extraction stress rather than the aging process itself. (lu.se)
Variants (also known as mutations) in the FGD1 gene are the only known genetic cause of Aarskog-Scott syndrome. (medlineplus.gov)

Mesenchymal stem cells (MSCs) support chondrogenic differentiation and are an attractive cell source for cartilage tissue engineering. (nature.com)
Patients with hypertrophic cardiomyopathy shown an increased expression of ALC-2 in whole heart tissue. (wikipedia.org)
Although mesenchymal stem cells (MSCs) have been shown as a novel approach in tissue regeneration, the therapeutic potential of MSCs mediated by the interaction between MSC-derived paracrine mediators and Mφs remains elusive. (frontiersin.org)
Methods: We analysed methylation status of 807 cancer-related genes in 189 fresh frozen primary breast tumours and four normal breast tissue samples using an array-based methylation assay. (lu.se)

Our study also suggests that BMP antagonists are candidate signaling components that make up the intestinal epithelial stem cell niche. (utmb.edu)
The abnormal wound healing is due to impaired limbal epithelial stem cells, and the new research, published online July 18 in Diabetologia , involved isolation of those cells from 30 donor eyes of humans with and 23 without diabetes. (medscape.com)
However, treatment with various approaches, including exogenous WNT5A methylation inhibitors and a nanoconjugate that inhibits WNT5A suppression, improved corneal epithelial wound healing as well as expression of the limbic epithelial stem cells. (medscape.com)

However, little information is available on the gene expression profile (GEP) of CMV-CTL clones according to CMV reactivation patterns (and donor CMV serostatus) after allo-HCT. (nature.com)
The volumetric analysis of cellular expression patterns provides evidence for molecular mechanisms regulating the development of pre-implantation mouse embryos. (zeiss.com)
Distinctive patterns of genes turned off -- or left on -- in healthy versus cancerous cells could enable early screening for many common cancers and maybe help avoid them scientists say. (sciencedaily.com)
Researchers are comparing chemical alterations, called DNA methylation, in the body's basic building block in healthy colon, breast, brain and lymphatic cells and their cancerous counterpart to find telltale patterns that could one day be detected in the blood, urine or feces. (sciencedaily.com)
Finally, by utilizing gene expression data, we observed that a large fraction of genes reported as having subtype-specific expression patterns might be regulated through methylation. (lu.se)

The key attributes like isolation efficiency, cell yield, colony-forming unit-fibroblast (CFU-F) frequency, phenotypic characteristics, and multi-lineage differentiation capacity were determined for the isolated MSCs from bone marrow specimens. (journalcra.com)
Apart from this, it was observed that those MSCs, when induced to differentiate into cardiomyocytes, they successfully differentiated into cardiomyocyte like cells with flat, wide, myotube like structure with sarcomeric organization, which were later confirmed to be cardiomyocytes by qRT-PCR. (journalcra.com)

Blood stem cells that were low in Sca-1 (and most prone to become red blood cells) had much more GATA1 than did the high- and medium-Sca-1 cells. (sciencedaily.com)
Blood stem cells, in particular, lose some of their functionality over time, contributing to various blood disorders and disease-related conditions. (lu.se)
Hematopoietic stem cells, known as blood stem cells, are crucial for producing all types of blood cells throughout a person's life. (lu.se)
Anna Konturek-Ciesla, a researcher at Lund Stem Cell Center and the lead author of the study explains, "In our research, which focuses on hematopoiesis, or blood cell formation, many studies have attempted to analyze the changes associated with aging in blood stem cells using gene expression profiling. (lu.se)
What makes blood stem cells transform? (lu.se)
Splicing factor mutations are particularly prevalent in MDS, a group of heterogeneous hematological disorders characterized by defective blood stem cells and a high risk of leukemia development. (lu.se)

Myeloid angiogenic cells (MAC) derive from hematopoietic stem/progenitor cells (HSPCs) that are mobilized from the bone marrow. (hindawi.com)
Such myeloid angiogenic cells (MAC) [ 1 ] of bone marrow origin were known as endothelial progenitor cells (EPCs). (hindawi.com)
It was observed that the bone marrow possessed a good number of nucleated cells and had a high yield of adherent cells. (journalcra.com)
Mesenchymal stem/progenitor cells (MSC/MPC) are found in many tissues and fluids including bone marrow, adipose tissues, muscle, synovial membranes, synovial fluid, and blood. (scirp.org)
Borlongan, C.V., Glover, L.E., Tajiri, N., Kaneko, Y. and Freeman, T.B. (2011) The Great Migration of Bone Marrow-Derived Stem Cells towards the Ischemic Brain: Therapeutic Implications for Stroke and Other Neurological Disorders. (scirp.org)
Normal hematopoiesis requires an intricate balance in the bone marrow between the ability of stem cells to maintain themselves for decades of life while producing billions of mature blood cells every day. (upenn.edu)
15]. The percentage of bone common of which are trisomy 8, mono- marrow blast cells for estimation of the Myelodysplastic syndrome (MDS) is somy 7 and 5q- [11]. (who.int)
Thus, BMP may play a role as a paracrine factor in the differentiation of satellite cells into bone-forming cells. (medscape.com)

Once you've made your decision to dig into the details, read more at Molecular Cell , May 2023 . (epigenie.com)
These cells expressed RPE-specific molecular markers and were capable of phagocytosis, an important RPE function. (news-medical.net)
Differentiomics could also be a valuable predictive tool for quality assessment of other ES cell derivatives based on their molecular signature. (news-medical.net)
Molecular mechanisms of the initiation of transcription from TATA box have been well known as the most essential nuclear events in mammalian cells. (intechopen.com)
Despite this wealth of knowledge, Anna Konturek-Ciesla noted that there is still no consensus about the molecular profile of aged hematopoietic stem cells. (lu.se)
When we looked at our data, we noticed a pattern of stress-induced changes in gene activity, especially in cells exposed to higher temperatures during isolation," explains David Bryder, Professor of Molecular Hematology at Lund University, and research group leader at Lund Stem Cell Center. (lu.se)
In a study published in the Molecular Cell journal, they explain what makes hematopoietic stem cells acquire malignant traits in cancer. (lu.se)
Introduction: Five different molecular subtypes of breast cancer have been identified through gene expression profiling. (lu.se)

Our study is aimed at investigating whether precursors of myeloid angiogenic cells (PACs) treated with known pharmaceuticals would produce media with better proangiogenic activity in vitro and if such media can be used to stimulate blood vessel growth in vivo . (hindawi.com)
Then, we used atorvastatin-stimulated cells or conditioned media from them in the Matrigel plug in vivo angiogenic assay. (hindawi.com)
We previously established a direct single-cell analysis to simultaneously identify and quantify in vivo CMV-CTL clones after allo-HCT 9 , 10 . (nature.com)
Our results suggest that ES cells recapitulate closely the early steps of muscle development in vivo and may serve as an excellent in vitro system to study this process. (uni-luebeck.de)
This is the first application of transcriptomics to assess ES cell derivatives and their in vivo counterparts -- a 'differentiomics' outlook. (news-medical.net)

Highly potent animal stem cells either self renew or launch complex differentiation programs, using mechanisms that are only partly understood. (bvsalud.org)
Our results provide an initial step to determine the mechanisms of action and to optimize cell therapy for SCI. (uab.cat)
His work investigating the neural mechanisms that control behaviour has made an impact across scales from cells to brain regions across mammalian species. (elifesciences.org)

Very similar changes also take place in the cells generated from fertilized eggs and in mammalian reproductive cells . (bvsalud.org)
Mammalian embryogenesis is a dynamic process involving gene expression and mechanical forces between proliferating cells. (lu.se)
The sphingomyelin that accumulates in the lysosomes of Niemann-Pick disease-affected cells is thought to arise from the degradation of the cells and their organelles because it is a major component of all mammalian cell membranes. (medscape.com)

Identified gene candidates will be studied further in the context of determining sensitivity of protein degrader drugs which are currently emerging as alternative therapies for leukemia. (lu.se)

Given that rRNA accounts for the bulk of the transcriptional output of the cell, the inability to map datasets to rDNA has historically been a major limitation, and has created a significant knowledge gap in our understanding of the most abundant RNA in the cell. (upenn.edu)
Given that OKSM (Yamanaka) factors convert somatic cells into induced pluripotent stem (iPS) cells, alterations in transcriptional state could affect destiny of the cells. (intechopen.com)
Their mutagenic potential and gene regulatory effect have shaped the evolution of transcriptional networks involved in development, pluripotency, and inflammation. (lu.se)

Relatively open GSC chromatin is further restricted by Polycomb repression of testis or somatic cell -expressed genes briefly active in early female germ cells . (bvsalud.org)

On the other hand, bulk RNA-sequence analyses reveal that gene expression profiles among patients are different according to the cytomegalovirus reactivation pattern, and are associated with cytokine production or cell division. (nature.com)

We have shown here that IM induces autophagy in CML blast crisis cell lines, CML primary cells, and p210BCR/ABL-expressing myeloid precursor cells. (lu.se)
Cells previously known as endothelial progenitor cells are considered now to be rather myeloid angiogenic cells than endothelial precursors [ 4 ]. (hindawi.com)
Therefore, the cells we used should be considered as hematopoietic precursors of myeloid angiogenic cells and are referred as PACs. (hindawi.com)

Most animals are made up of two cell types germline stem cells , which give rise to reproductive cells ( egg and sperm ) and pass their DNA to the next generation, and somatic cells , which make up the rest of the body. (bvsalud.org)
An individual T-cell has a specific complementarity-determining region 3 (CDR3) of T cell receptor (TCR)-α and -β, which is a result of the recombination of somatic TCR V-(D)-J genes and junction diversity. (nature.com)

Identification of Gene Expression-Based Prognostic Markers in the Hematopoietic Stem Cells of Patients With Myelodysplastic Syndromes. (stembook.org)

Understanding how cancer stem cells affect tumor heterogeneity, the contribution of resident stem and progenitor cells to the tumor niche, and the search for true cancer stem cell markers are other examples of scientific challenges in this fast-growing research area. (hindawi.com)
We invite investigators to submit original research as well as review articles addressing recent advancements in our understanding of stem cells in cancer. (hindawi.com)
Understanding how embryonic cells biomolecularly restrict their early developmental potential and promote specific cell commitment helps to provide key insights on cancer plasticity and could improve fertilization protocols. (zeiss.com)
DNA methylation is a piece of a relatively new research field called epigenetics that looks more globally at which genes are turned off and on with an eye on early identification of some of the aberrant adjustments that enable cancer cells to thrive. (sciencedaily.com)
A lot of methylated genes we found in cancer are not naturally expressed in normal cells. (sciencedaily.com)
A lot of people think of cancer cells turning genes on, like oncogenes, that allow them to grow faster, evade immune surveillance, that sort of thing," Dr. Robertson says. (sciencedaily.com)
Induction of cancer-associated fibroblast-like cells by carbon nanotube s dictates its tumorigenicity. (cdc.gov)
The mechanism by which CNT-induced CAF-like cells promote tumor growth involved the acquisition of cancer stem cells (CSCs) in cancer population. (cdc.gov)
Perioperative Durvalumab for Resectable Non-Small-Cell Lung Cancer. (cdc.gov)

Single-cell RNA-sequence analyses demonstrate the homogenous distribution of the gene expression profile in individual cytomegalovirus-specific cytotoxic T-cells within each recipient. (nature.com)

Hypoxic stem cell lines showed a clear time-dependent activation of the entire panel of pro-inflammatory genes as compared to differentiated tumor cells. (biomedcentral.com)
Because tumor cells that result from aberrant changes shed their DNA into bodily fluids, non-invasive screening for a wide range of cancers could result be another result of this initiative, Dr. Bhalla says. (sciencedaily.com)

With therapeutic cloning, or the creation of banks of stem cells generated using parthenogenesis (perhaps as few as 40-400 lines), a limitless supply of immune-matched cells could be generated for most of the population. (news-medical.net)
The work also suggests a much more efficient way of differentiating stem cells for therapeutic purposes. (sciencedaily.com)

Atrial Light Chain-2 (ALC-2) also known as Myosin regulatory light chain 2, atrial isoform (MLC2a) is a protein that in humans is encoded by the MYL7 gene. (wikipedia.org)
An important next step will be to test the ability of these cells to restore visual function in both humans and animal models. (news-medical.net)
2002). In humans, SSEA4 is expressed by building the nervous system but also for their prospec- nonneural cells such as the erythrocytes (Kannagi et al. (lu.se)

Additionally, we sought to identify hEB markers that could be used to determine the presence of differentiated cells in specific tissues, allowing for the purification of homogeneous cell populations or serving as indicators of hESC differentiation. (molcells.org)
The findings, published in the May 22 issue of Nature, give a glimpse into how that collective behavior works, and show that cell populations maintain a built-in variability that nature can harness for change under the right conditions. (sciencedaily.com)

In vitro analysis demonstrated that gremlin 1 partially inhibits Caco-2 cell differentiation upon confluence and activates Wnt signaling in normal rat intestinal epithelial cells. (utmb.edu)
The FGD1 gene provides instructions for making a protein that turns on (activates) another protein called Cdc42, which transmits signals that are important for various aspects of development before and after birth. (medlineplus.gov)

Now, Harrison Gabel's lab (Washington University School of Medicine in St. Louis) reports that two phenomena-gene topology and gene expression-are tied together through NSD1 , which directs non-CG methylation to the right places. (epigenie.com)
A tumor is not only gaining methylation in certain areas and turning this gene off, it's losing methylation in normally methylated areas," echoes Dr. Robertson. (sciencedaily.com)
Significant differences were found in DNA methylation between the cells of those two groups. (medscape.com)

We used G-CSF-mobilized CD34 + HSPCs, FACS-sorted from healthy donor peripheral blood mononuclear cells (PBMCs). (hindawi.com)

The conventional view of how stem cells differentiate assumes that cells are "instructed" to progress along prescribed signaling pathways. (sciencedaily.com)
A new study suggests that the conventional view, which assumes that cells are "instructed" to progress along prescribed signaling pathways, is too simplistic. (sciencedaily.com)

Anatomy55

Organisms16

Diseases9

Chemicals and Drugs54

Analytical, Diagnostic and Therapeutic Techniques and Equipment51

Phenomena and Processes69

Disciplines and Occupations5

Technology, Industry, Agriculture1

Information Science5

Health Care3

Mesenchymal stem5

Pluripotent stem13

Biology12

Proteins7

Proliferation6

Regenerative medicine4

Regulation8

Transplantation9

Progenitors2

Marker10

Genome5

Leukemic cells2

Epigenetic4

Characterization1

Abstract1

Phenotype7

Stromal cells3

Differentially1

Human32

Apoptosis4

Angiogenesis1

Embryonic stem4

Transcripts3

Maturation2

Functional3

Regulate4

Genetic4

Tissue4

Epithelial stem3

Patterns5

MSCs2

Blood stem cells6

Bone8

Molecular8

Vivo5

Mechanisms3

Mammalian3

Leukemia1

Transcriptional3

Chromatin1

Profiles1

Myeloid3

Somatic2

Myelodysplastic Syndromes1

Cancer9

Analyses1

Tumor cells2

Therapeutic2

Humans3

Populations2

Activates2

Methylation3

Donor1

Pathways2