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  • embryonic stem
  • Engineering integrin signaling for promoting embryonic stem cell self-renewal in a precisely defined niche," Biomaterials , vol. 31, no. 6, pp. 1219-1226, 2010. (hindawi.com)
  • The role of PI3K/AKT, MAPK/ERK and NF κ β signalling in the maintenance of human embryonic stem cell pluripotency and viability highlighted by transcriptional profiling and functional analysis," Human Molecular Genetics , vol. 15, no. 11, pp. 1894-1913, 2006. (hindawi.com)
  • colony-forming unit-e
  • One of its roles is to change the BFU-E (burst-forming unit-erythroid) cells, which are the earliest erythrocyte precursors in the erythrocytic series, into the CFU-E (colony-forming unit-erythroid). (prospecbio.com)
  • Here, we show that TET2 deficiency leads initially to stem cell factor (SCF)-dependent hyperproliferation and impaired differentiation of human colony-forming unit-erythroid (CFU-E) cells, which were reversed by a c-Kit inhibitor. (bloodjournal.org)
  • malignant
  • Testicular germ-cell tumors (TGCTs) of adolescents and adults originate from intratubular germ cell neoplasia (ITGCN), which is composed of the malignant counterparts of embryonal germ cells. (aacrjournals.org)
  • However, oncogenic cells bypass this process due to the lack of oncogene-induced senescence effectors, such as p16 or p53 ( 1 ), and establish malignant transformation. (aacrjournals.org)
  • For example, application to malignant cells of the "histone code" hypothesis, which posits that different combinations of histone modifications mediate unique cellular responses, suggests that abnormal histone modifications in cancers serve as one of the molecular switches for malignant transformation. (aacrjournals.org)
  • SALL4 expression was higher in SP cells compared to non-SP cells by 2-4 fold in various malignant hematopoietic cell lines. (harvard.edu)
  • mesenchymal
  • The effects of LNC transplantation for alkali burn limbal stem cell defect model were tested using bone marrow derived mesenchymal stem cell (BM-MSC) as control. (arvojournals.org)
  • Increased stemness and migration of human mesenchymal stem cells in hypoxia is associated with altered integrin expression," Biochemical and Biophysical Research Communications , vol. 423, no. 2, pp. 379-385, 2012. (hindawi.com)
  • vascular endoth
  • Beppu K, Jaboine J, Merchant MS, Mackall CL, Thiele CJ (2004) Effect of imatinib mesylate on neuroblastoma tumorigenesis and vascular endothelial growth factor expression. (springer.com)
  • mediates
  • SCF also mediates cell-cell adhesion and acts synergistically with other cytokines. (biosensis.com)
  • Hypoxia-inducible factor 1 α mediates anoikis resistance via suppression of α 5 integrin," Cancer Research , vol. 68, no. 24, pp. 10113-10120, 2008. (hindawi.com)
  • cytokine
  • Further, the relative level of tyrosine phosphorylation of various substrates by either cytokine alone differs from that produced by the combination of the two cytokines, suggesting that cell signaling by the combination of the two cytokines differs from that produced by either alone. (nih.gov)
  • protein
  • The cells were lysed, and equal amounts of protein from each lysate were separated by SDS/PAGE and electrotransferred to Immobilon P, followed by immunoblotting with the indicated antibodies. (nih.gov)
  • Polycomb protein Bmi1, which is a potent negative regulator of the p16INK4 gene, suppresses senescence in primary cells and is overexpressed in various cancers. (aacrjournals.org)
  • Since SCF immunoreactivity normally accumulates in basolateral compartments of SCF-expressing embryonic epithelial tissues, we analyzed the localization of wildtype and Sl17H mutant SCF protein in transfected epithelial (MDCK) cells in vitro. (eurekamag.com)
  • Human - A DNA sequence encoding the signal peptide and the mature Stem Cell Factor (SCF) protein of human SCF was expressed in modified human 293 cells. (biosensis.com)
  • expression
  • Its expression in these cells increases in response to TBI, the researchers said. (medicalxpress.com)
  • The expression of stem cell factor (SCF) and C-kit in human and mouse limbus niche were examined with immune histochemical staining and western blot. (arvojournals.org)
  • The expression of SCF in cultured human limbal niche cell (LNC) was examined with Immunofluorescence staining, western blot and ELISA. (arvojournals.org)
  • In addition, while overexpression of SALL4 led to drug resistance in cell lines, cells with decreased SALL4 expression were more sensitive to drug treatments than the parental cells. (harvard.edu)
  • In the absence of later acting factors such as GM-CSF, cells that progress beyond the CFU-GM stage lose c-kit expression and die by default. (mysciencework.com)
  • Others[ 9 ] showed that Bmi-1 expression in CD34+ cells was high in refractory anemia with excess blasts (RAEB), RAEB in transformation (RAEB-T), and MDS-AML. (biomedcentral.com)
  • Gene expression signature for angiogenic and nonangiogenic non-small-cell lung cancer," Oncogene , vol. 24, no. 7, pp. 1212-1219, 2005. (hindawi.com)
  • SALL4
  • This led to our investigation of the implication of SALL4 in drug resistance and its role in side population (SP) cancer stem cells. (harvard.edu)
  • Knocking down of SALL4 in isolated SP cells resulted in a reduction of SP cells, indicating that SALL4 is required for their self-renewal. (harvard.edu)
  • Taken together, our results suggest a novel role for SALL4 in drug sensitivity, at least in part through the maintenance of SP cells, and therefore may be responsible for drug-resistance in leukemia. (harvard.edu)
  • We are the first to demonstrate a direct link between stem cell factor SALL4, SP and drug resistance in leukemia. (harvard.edu)
  • gene
  • In this study, the researchers found that conditional deletion of Sox2 - the gene encoding the SOX2 stem cell transcription factor - and the associated dampening of astrocyte reactivity appear to promote functional recovery, including behavioral recovery, after traumatic brain injury , said Dr. Zhang, a W.W. Caruth, Jr. Scholar in Biomedical Research. (medicalxpress.com)
  • Sickle cell anemia is a genetic disorder that results from a single nucleotide substitution in codon 6 of the beta-globin gene which, in the homozygous state, produces an abnormal hemoglobin that is prone to polymer formation when deoxygenated. (clinicaltrials.gov)
  • Celladon Corporation, a clinical-stage biotechnology company applying its leadership position in the field of gene therapy and calcium dysregulation today announced an exclusive, global license from Enterprise Partners Venture Capital for gene therapy applications of the membrane-bound form of the Stem Cell Factor gene (mSCF) for treatment of cardiac ischemia. (stemcellsfreak.com)
  • mSCF gene therapy promoted a regenerative response in damaged hearts similar to that observed with stem cell therapy and may be one of the first non-cell therapies to increase cardiac muscle precursors in the heart. (stemcellsfreak.com)
  • We believe mSCF gene therapy has the potential to be a powerful therapeutic approach, harnessing the potency of stem cell therapy without the associated complications of developing cells as products. (stemcellsfreak.com)
  • imatinib
  • Although cisplatin treatment eliminated the majority of tumor cells, it did not eliminate CSCs, whereas imatinib or anti-SCF antibody destroyed CSCs. (aacrjournals.org)
  • precursors
  • The normal products of the murine Steel (Sl) and Dominant white spotting (W) genes are essential for the development of melanocyte precursors, germ cells, and hematopoietic cells. (eurekamag.com)
  • We suggest, therefore, that the loss of melanocyte precursors prior to epidermal invasion, and the loss of germ cells from mature testis, can be explained by the inability of Sl17H mutant SCF to be targeted to the basolateral compartment of polarized epithelial keratinocytes and Sertoli cells, respectively. (eurekamag.com)
  • tumors
  • In the two wild-type bilateral tumors for which ITGCN was available, the preinvasive cells contained the mutation. (aacrjournals.org)
  • They are drug resistant and display the ability to self-renew and generate a progeny of the differentiated cells that constitute a large majority of the cells in tumors. (aacrjournals.org)
  • Targeting
  • Our study demonstrates the role of stem cell factor (SCF)-based toxin conjugates for targeting WT-KIT-overexpressing malignancies such as NBs and CRCs. (springer.com)
  • Efficient targeting was achieved in all receptor-positive neuroblastoma (IMR-32 and SHSY5Y) and colon cancer cell lines (COLO 320DM, HCT 116, and DLD-1) but not in receptor-negative breast carcinoma cell line (MCF-7) thereby proving specificity. (springer.com)
  • cultures
  • EML cultures contain a heterogeneous mixture of cells, including a lineage-negative, CD34+ subset of cells that propagate rapidly in SCF and can clonally regenerate the mixed population. (nih.gov)
  • The percentage of cell types in the human rIL-3 and human rIL-3 plus human rSCF cultures, however, remained the same, with basophils constituting 18 to 35% of the final cultured cells, and mast cells 3% or less of the final cell number. (jimmunol.org)
  • Electron microscopic examination of cultures supplemented with human rIL-3 and rSCF, but not human rIL-3 alone, revealed that after 3 wk in culture, mast cell granules contained tryptase and exhibited scroll, reticular, and homogeneous patterns as seen previously in CD34+/3T3 fibroblast cocultures. (jimmunol.org)
  • Thus, CD34+ cells cultured in the presence of both human rIL-3 and rSCF give rise to cultures containing increased numbers of basophils and mast cells, with the mast cells by ultrastructural studies showing evidence of maturation although the percentages of basophils and mast cells arising in these cultures remained unchanged. (jimmunol.org)
  • On transfer to GM-CSF containing cultures, these cells proliferate and differentiate terminally. (mysciencework.com)
  • Alzheimer's
  • Astrocytes, the supporting cells of the brain, could play a significant role in the pathogenesis of Alzheimer's disease (AD), according to a new study from the University of Eastern Finland. (medicalxpress.com)
  • vivo
  • These data demonstrate that administration of SCF in vivo affects extrathymic-origin thymocyte regenerating cells and may influence, directly or indirectly, early prethymic stages of T-cell lymphopoiesis in addition to its known effect on early stages of myelopoiesis and erythropoiesis. (hindawi.com)
  • We now report that administration of SCF intradermally in vivo results in dermal mast cell activation and a mast cell-dependent acute inflammatory response. (rupress.org)
  • synergistically
  • The response of these cells to IL-3 is stimulated synergistically by SCF, and we present evidence that both the synergy and the inhibition of c-kit responses may be mediated by direct interaction with IL-3 receptor. (nih.gov)
  • Over 3 to 4 wk, human rSCF acted synergistically with human rIL-3 at all concentrations, producing a three- to fivefold increase in total, mast cell, and basophil numbers over human rIL-3 alone when used at 100 ng/ml. (jimmunol.org)