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  • centrosomes
  • Disruption of centrosomes disrupts the high fidelity of asymmetric division. (rupress.org)
  • Jens Januschke and Cayetano González , both scientists at the Institute for Research in Biomedicine (IRB Barcelona), have discovered that microtubules and centrosomes, organelles that govern some cell division processes, participate in memorizing this orientation over successive generations. (healthcanal.com)
  • Localization of Giα proteins in the centrosomes and at the midbody: implication for their role in cell division. (springer.com)
  • The cystoblast then divides and matures into an oocyte, a process in which centrosomes exhibit a series of complex behaviors proposed to play a crucial role in oogenesis [4- (ox.ac.uk)
  • REVIEW Reina J., Gonzalez C. (2014) When fate follows age: unequal centrosomes in asymmetric cell division. (irbbarcelona.org)
  • tumor
  • Thus, mechanistic insight into how the sibling cells assume distinct identities during asymmetric stem cell division is likely to advance our knowledge in stem cell biology, developmental biology and tumor biology. (biologists.org)
  • Metastasis is a process in which tumor cells shed from the primary tumor intravasate blood vascular and lymphatic system, thereby, gaining access to extravasate and form a secondary niche. (jove.com)
  • The extravasation of tumor cells from the blood vascular system can be studied using endothelial cells (ECs) and tumor cells obtained from different cell lines. (jove.com)
  • However, to observe and study interactions with rare cells such as circulating tumor cells (CTCs), certain changes are required to be made to the conventional flow chamber assembly. (jove.com)
  • Cancer stem cells (CSCs) are notorious for their capacity of tumor progression, metastasis or resistance to chemo-radiotherapy. (biomedcentral.com)
  • One daughter remains a it seems more likely that a tumor would arise from a normal stem cell and the other gives rise to differentiated progeny that stem cell. (scribd.com)
  • Primary mammary epithelial cell cultures are generated from mammary glands without palpable tumor. (jove.com)
  • Despite the outsized medical impact of metastasis, remarkably little is known about one of the key early steps of metastasis: departure of a tumor cell from its originating tissue. (sdbonline.org)
  • proteins
  • Major changes in transcription, morphology, and function occur at the basal/spinous layer transition and again at the granular/stratum corneum transition such that differentiated cells reaching the skin surface are enucleated cellular skeletons that are packed with cables of keratin filaments encased by a γ-glutamyl-ε-lysine cross-linked cornified envelope of proteins. (rupress.org)
  • We will produce the proteins responsible for carrying these signals and get them to work outside of the cell. (wellcome.ac.uk)
  • T cells accomplish regulated adhesion by controlling the adhesive properties of integrins, a class of cell adhesion molecules consisting of heterodimeric pairs of transmembrane proteins that interact with target molecules on partner cells or extracellular matrix. (jove.com)
  • SIGNIFICANCE:B cell lymphoma-2 (Bcl-2) was discovered over three decades ago and is the prototype antiapoptotic member of the Bcl-2 family that comprises proteins with contrasting effects on cell fate. (nus.edu.sg)
  • Our cells have a lot to do, and the proteins inside of them need to work together to get it all done. (uoregon.edu)
  • We have studied the Par complex, a set of three proteins that polarizes many different cell types, including neuroblasts. (uoregon.edu)
  • Interfering with the function of these proteins in vitro randomizes spindle orientation and changes cell fate. (biologists.org)
  • Some of the complexity in the Pn.p cell fusion pattern arises because these two Hox proteins can regulate each other s activities. (sdbonline.org)
  • REF-2 functions with the Hox proteins to prevent Pn.p cell fusion. (sdbonline.org)
  • Next, Wang plans to investigate the other proteins that regulate microtubule growth and their role in asymmetric cell division. (duke-nus.edu.sg)
  • Pins-like proteins and Gα subunits are key components of a highly conserved molecular machinery that links the cell cortex to the astral microtubules of the mitotic spindle ( Siller and Doe, 2009 ). (biologists.org)
  • protein
  • Consistently, Klu protein is detected in type II neuroblasts but is undetectable in immature INPs. (biologists.org)
  • The G-protein regulatory (GPR) motif-containing Leu-Gly-Asn-enriched protein (LGN) and Giα3 influence cortical positioning of the mitotic spindle poles at metaphase in symmetrically dividing mammalian cells. (springer.com)
  • Here we report the identification of a gene, miranda, which encodes a new protein that co-localizes with Prospero in mitotic neuroblasts, tethers Prospero to the basal cortex of mitotic neuroblasts, directing Prospero into the GMC, and releases Prospero from the cell cortex within GMCs. (eurekamag.com)
  • aPKC (an apical protein in red), Mira (a basal protein in green) and DNA (in blue) were labeled in wild-type neural stem cells. (duke-nus.edu.sg)
  • Recent studies in mammalian tissue culture cells suggest that the apically localised atypical protein kinase C (aPKC) is important for the planar orientation of the mitotic spindle in dividing epithelial cells. (biologists.org)
  • Here, we show that expression of the proneural protein Lethal of scute [L(1)sc] signals the transition of NE cells to NBs in the OOA. (biologists.org)
  • molecular
  • Studies aimed at dissecting the molecular mechanism involved in stem cell regulation have been hampered by difficulties in obtaining populations of HSCs devoid of more differentiated progenitor cells. (rupress.org)
  • We will prove their functions in learning and study in detail at molecular levels how dopamine modulates the connection between neuronal cells. (wellcome.ac.uk)
  • POU-III transcription factors (Brn1, Brn2, and Oct6) influence neurogenesis, molecular identity, and migratory destination of upper-layer cells of the cerebral cortex. (springer.com)
  • basal cell
  • In human epidermis, the self-renewing capacity of epidermal stem cells is enormous, and, within 4 wk, a basal cell has terminally differentiated and exited at the skin surface. (rupress.org)
  • progenitors
  • Thus, we have identified proliferative characteristics and a dose-dependent transcriptional network that define distinct OE precursors: medial precursors that are most probably transit amplifying neurogenic progenitors for ORNs, VRNs and GnRH neurons, and lateral precursors that include multi-potent self-renewing OE neural stem cells. (biologists.org)
  • To overcome these problems we have studied the optically superior brain of the zebrafish embryo and have monitored the in vivo behaviour of fluorescently labelled neural progenitors and their daughter cells throughout a substantial period of hindbrain development. (biologists.org)
  • However, the neuroblast division generates two progenitors, whereas in vertebrate studies an asymmetric division is typically defined as one that generates a progenitor and a neuron (e.g. (biologists.org)
  • tissue
  • Tissue stem cells play a key role in tissue maintenance. (rupress.org)
  • Adult tissue stem cells play key roles in tissue maintenance/repair ( Nystul and Spradling, 2006 ). (rupress.org)
  • The skin's elixir for maintaining tissue homeostasis, regenerating hair, and repairing the epidermis after injury is its stem cells, which reside in the adult hair follicle, sebaceous gland, and epidermis. (rupress.org)
  • Literally meaning 'big eater', macrophages capture and destroy microbial intruders, but also clear dead cells and orchestrate tissue repair after injury or infection. (wellcome.ac.uk)
  • Our new special issue is packed with articles that use mathematical and physical approaches to gain insights into cell and tissue patterning, morphogenesis and dynamics, and that provide a physical framework to capture these processes operating across scales. (biologists.org)
  • Glands are carefully resected with clear separation from adjacent muscle, lymph nodes are removed, and single-cell suspensions of enriched mammary epithelial cells are generated by mincing mammary tissue followed by enzymatic dissociation and filtration. (jove.com)
  • Simple epithelial repair relies on a combination of collective cell movements and the action of a contractile actomyosin cable at the wound edge that together promote the fast and efficient closure of tissue discontinuities. (sdbonline.org)
  • Popcorn (W Pop ), a virulent strain of Wolbachia , provides a particularly striking demonstration of the role of Wolbachia -specific factors as it over-replicates in adult neurons and muscle cells ultimately causing tissue degeneration and premature death ( Min and Benzer, 1997 ). (biologists.org)
  • Milk fat globule-epidermal growth factor-factor VIII (MFG-E8) is a secretory glycoprotein that plays a wide range of cellular functions including phagocytosis of apoptotic cells, anti-inflammation, tissue regeneration, and homeostasis. (frontiersin.org)
  • transcription
  • Basal cells express several characteristic markers, including keratins and transcription factors. (rupress.org)
  • Ikaros, an early lymphoid-specific transcription factor and a putative mediator for T cell commitment. (springer.com)
  • Early in development, neuroblasts switch through a temporal series of transcription factors modulating neuronal fate according to the time of birth. (biologists.org)
  • This raises the issue of whether there are additional temporal transcription factors expressed in neuroblasts during postembryonic stages. (biologists.org)
  • A zinc-finger transcription factor, REF-2, is described that is required for the Pn.p cells to be generated and to remain unfused. (sdbonline.org)
  • biology
  • The study has been published in the first issue of March of the Journal of Cell Biology , edited by Rockefeller University Press . (healthcanal.com)
  • The postdoc work of Nicolas Loyer in the Januschke lab, Division of Cell and Developmental Biology, recently published in Nature Communications, addresses this question. (dundee.ac.uk)
  • In this biobytes podcast, Duke-NUS' very own Assoc Prof Wang Hongyan discusses her recent publication in Journal of Cell Biology that reports the role of ADP ribosylation factor like 2 ( arl2 ) and mini spindles ( msps ) genes in asymmetric cell division. (duke-nus.edu.sg)
  • Journal of Cell Biology. (irbbarcelona.org)
  • The study, published in The Journal of Cell Biology, focuses on the development of the sperm tail, the structure that enables sperm cells to swim and is therefore critical for male fertility. (irbbarcelona.org)
  • L'estudi, publicat a The Journal of Cell Biology , se centra en el desenvolupament de la. (irbbarcelona.org)
  • embryo
  • Cells in the early embryo are of particular interest as they harbour the potential to form all cell types found in the adult body. (wellcome.ac.uk)
  • From stem cell to embryo without centrioles. (ox.ac.uk)
  • The genetic analysis of the alpha Tub67C gene demonstrates that it is required for nuclear division in the oocyte and early embryo. (sdbonline.org)
  • aPKC
  • Yet, in chicken neuroepithelial cells, aPKC is not required in vivo for spindle orientation, and it has been proposed that the polarization cues vary between different epithelial cell types and/or developmental processes. (biologists.org)
  • brains
  • Aberrantly increasing Arm activity in immature INPs further exacerbates the defects in the specification of INP identity and enhances the supernumerary neuroblast mutant phenotype in brat mutant brains. (biologists.org)
  • By contrast, reducing Arm activity in immature INPs suppresses supernumerary neuroblast formation in brat mutant brains. (biologists.org)
  • We also detail our simplified protocol used to explant whole brains from third instar larvae for live cell imaging and fixed analysis applications. (jove.com)
  • regulate
  • We will use fruit flies to find neuronal cells that regulate the release of dopamine and produce an anatomical database and genetic tools to manipulate them. (wellcome.ac.uk)
  • The ability of a T cell to control adhesion derives from the ability to regulate the affinity states of individual integrins. (jove.com)
  • Wnt genes encode secreted factors that regulate various cell fate decisions depending on the cellular context. (biologists.org)
  • Zhang Y., Rai M., Wang C., Gonzalez C., Wang H. (2016) Prefoldin and Pins synergistically regulate asymmetric division and suppress dedifferentiation. (irbbarcelona.org)
  • distinct
  • Our results indicate that these cells originate in three distinct clusters in the dorsal protocerebrum during stage 12 of embryonic development. (biologists.org)
  • Migrating cells polarize in the direction of movement, creating distinct leading and trailing structures. (biologists.org)
  • developmental
  • We suggest using genome editing to mark homologous neural cells in the fly D. melanogaster , the beetle Tribolium castaneum , and the Mediterranean field cricket Gryllus bimaculatus to investigate developmental differences leading to brain diversification. (springer.com)
  • tissues
  • Stem cells have the remarkable capacity to both self-perpetuate and also give rise to the differentiating cells that constitute one or more tissues. (rupress.org)
  • All tissues of the body are populated with a mixture of immune cells that help protect the body from invasion by microbes. (wellcome.ac.uk)
  • In many tissues the most abundant immune cell is the macrophage. (wellcome.ac.uk)
  • During vertebrate development, a wide variety of cell types and tissues emerge from a single fertilized oocyte. (embopress.org)
  • This Review presents the latest advances in our understanding of stem cell aging in multiple tissues, and discusses their implications for lifespan, disease and potential therapies. (biologists.org)