Stem cell differentiation regulates satellite cell differentiation skeletal muscle proliferation and differentiation. Medical search. Frequent questions

As one of the key cell markers of satellite cells (the stem cell pool for skeletal muscles), it plays an important role in the regeneration of adult muscle. (wikipedia.org)
Cellular and molecular regulation of muscle regeneration. (scielo.br)
Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)
Remarkably type 2 cytokine signaling is also required in FAPs but not myeloid cells for quick clearance of necrotic debris a process that is necessary for timely and total regeneration of BV-6 cells. (scienceexhibitions.org)
2008 In addition to satellite cells recent studies have recognized an important part for fibro/adipogenic progenitors (FAPs) in muscle mass regeneration and its fatty degeneration (Joe et al. (scienceexhibitions.org)
In a number of species cells regeneration is associated with the presence of the molecular signature for type 2 innate immune response such as alternatively triggered (M2) macrophages and eosinophils (Allen and Wynn 2011 Palm et al. (scienceexhibitions.org)
Low-intensity pulsed ultrasound (LIPUS, a form of mechanical stimulation) can promote skeletal muscle functional repair, but a lack of mechanistic understanding of its relationship and tissue regeneration limits progress in this field. (ijbs.com)
We investigated the hypothesis that specific energy levels of LIPUS mediates skeletal muscle regeneration by modulating the inflammatory microenvironment. (ijbs.com)
LIPUS at intensity of 60mW/cm 2 could significantly promoted skeletal muscle regeneration through shifting macrophage phenotype from M1 to M2. (ijbs.com)
Additionally, we are interested in mechanisms responsible for skeletal muscle recovery and regeneration after injury. (uncg.edu)
More specifically, my laboratory investigates the role inflammation plays in muscle atrophy, as well as during recovery and regeneration after injury. (uncg.edu)
Aged skeletal muscle displays increased fibrosis and impaired regeneration. (uncg.edu)
In aged skeletal muscle, impairments in regrowth and regeneration may be explained by a decreased responsiveness of muscle precursor cells (MPCs) to environmental cues such as growth factors. (uncg.edu)
Our team is working on the molecular and cellular actors involved in human muscle regeneration , in muscle ageing and in muscular dystrophies including oculopharyngeal muscular dystrophy (OPMD) and Duchenne muscular dystrophy (DMD). (institut-myologie.org)
More precisely we are working on RNA metabolism , muscle regeneration , muscle stem cells , and fibrosis , with the final aim of developing innovative therapeutic approaches. (institut-myologie.org)
In 2009 beginning of 2010 we have focused on investigating what factors human embryonic stem cells (hESCs) may produce that enhance regeneration and if those factors have any effects by themselves on regeneration. (ca.gov)
Our current running hypothesis is that the positive growth factors produced by hESCs trigger injured muscle to initiate and maintain regeneration, the TGF-beta inhibitors produced by hESCs reduce the TGF-beta signaling, and the combination assures the robust regeneration of muscle. (ca.gov)
Preliminary data indicates the effects on regeneration of old muscle look very promising. (ca.gov)
Specifically, our data suggest that several other ligands of MAPK pathway secreted by hESCs are likely to enhance and rejuvenate the regeneration of old muscle tissue. (ca.gov)
We have submitted the several manuscripts on topics of enhanced tissue regeneration and we are preparing the manuscript that identifies hESC-based novel strategies for restoring high regenerative capacity to old muscle. (ca.gov)
Finally, our data suggest that muscle stem cells either do not accumulate DNA damage with age or can efficiently repair such damage, when activated for tissue regeneration. (ca.gov)
Satellite cells and skeletal muscle regeneration, Compr. (copernicus.org)
Skeletal muscle growth and regeneration involves the activity of resident adult stem cells, namely satellite cells (SC). (sdbonline.org)
Collectively, this study provides the first evidence that transient levels of the evolutionarily conserved factor RACK1 are critical for adult SC activation and proper skeletal muscle regeneration, favoring the efficient progression of SC from a committed to a fully differentiated state. (sdbonline.org)
These cells play a vital role in muscle repair and regeneration. (2014davidsonfellows.com)
Conditions involving muscles wasting such as for example muscular dystrophies cachexia and sarcopenia would reap the benefits of strategies URMC-099 that promote skeletal muscles regeneration. (biotech-angels.com)
Macrophages later arrive at the tissue to remove cellular debris, and satellite cells (SCs) are activated and proliferate to induce myofiber regeneration. (biomedcentral.com)
Therefore, medications that can prevent the progression of muscle atrophy or promote muscle regeneration are in high demand. (biomedcentral.com)
Considering that reinnervation of the TA muscle can occur even after UVFP through these anastomoses, it would be ideal if drugs that promote nerve regeneration could be used to treat UVFP. (biomedcentral.com)
Satellite cells (SCs), which are precursors of skeletal muscles, such as the TA muscle, play a key role in muscle homeostasis and regeneration. (biomedcentral.com)
SCs are normally in a quiescent state in adult muscles, and start proliferating during muscle regeneration in response to denervation. (biomedcentral.com)

A new tumor model, combining human embryonic stem cells (ESC) and tumor cells, develops abundant human vessels. (nih.gov)
TATA-box binding protein is not required for RNA Polymerase II transcription in mouse embryonic stem cells. (elifesciences.org)
2022. Transcriptional programs regulating neuronal differentiation are disrupted in DLG2 knockout human embryonic stem cells and enriched for schizophrenia and related disorders risk variants . (cardiff.ac.uk)

It is a protein with a key role in regulating muscle differentiation or myogenesis, specifically the development of skeletal muscle. (wikipedia.org)
In zebrafish, Myf5 is the first MRF expressed in embryonic myogenesis and is required for adult viability, even though larval muscle forms normally. (wikipedia.org)
As no muscle is formed in Myf5;Myod double mutant zebrafish, Myf5 cooperates with Myod to promote myogenesis. (wikipedia.org)
Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
MicroRNA-1/206(miRNA-1/206) is specifically expressed in skeletal muscle and play a critical role in myogenesis. (biomedcentral.com)
Activation of IL-4/IL-13 signaling promotes proliferation of FAPs to support myogenesis while inhibiting their differentiation into adipocytes. (scienceexhibitions.org)
Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD. (chinagene.cn)

Although it is expressed in both the epaxial (to become muscles of the back) and hypaxial (body wall and limb muscles) portions of the myotome, it is regulated differently in these tissue lines, providing part of their alternative differentiation. (wikipedia.org)
More proliferation was observed in muscle tissue of LBW-GLN than LBW-ALA piglets at 5 dpn, but there was no clear effect of supplementation on related gene expression. (nature.com)
Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
Glutamine is regarded as the most abundant amino acid in the body, is mainly synthesized in skeletal muscle 10 , and muscle tissue is the most important site for Gln storage 11 . (nature.com)
Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
2010 However reflecting their adipogenic potential FAPs can also give rise to ectopic adipocytes that accumulate in degenerating muscle tissue (Uezumi et al. (scienceexhibitions.org)
Muscle injury results in quick activation of the innate immune system which exerts pleiotropic effects on regenerating muscle mass (Brunelli and Rovere-Querini 2008 Tidball and Villalta 2010 Within minutes of injury neutrophils infiltrate hurt skeletal muscle mass and launch tissue-damaging reactive molecules which exacerbate muscle mass damage (Tidball 1995 This initial burst of security damage caused by the innate immune system is followed by a wave of reparative macrophages. (scienceexhibitions.org)
In this paper, we discuss the current available information about the role that different phenotypes of macrophages plays after injury and during the remodelling phase in different tissue types, with particular attention to the skeletal muscle. (hindawi.com)
A beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion in mice. (omicsdi.org)
The results support the concept that fuel excess can drive obesity and diabetes via hyperinsulinaemia, and that an islet beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion. (omicsdi.org)
However, since there are many different non-muscle cell populations that reside within the tissue, these experiments cannot easily distinguish true myonuclei from those of neighboring mononuclear cells. (frontiersin.org)
Skeletal muscle is the largest tissue in the body. (frontiersin.org)
In both processes, rapid proliferation gives rise to new tissue, cell fate has to be specified within that tissue, and distinct positional identities have to be established to generate a properly patterned structure. (frontiersin.org)
Tissue-engineering and cell-based therapies, two prominent branches of Regenerative Medicine, are considered as the most suitable techniques to repair the tissue damage. (sciepub.com)
It's actually a special type of tissue in your body, where cells are fused together extra close - so close, that they behave a like a giant single cell. (robert-gorter.info)
This assumption long seemed valid, with many researchers reporting the presence of disintegrating nuclei in muscle tissue during atrophy induced by inactivity, injury or paralysis. (robert-gorter.info)
The response of target cells is further regulated by their microenvironment, involving the extracellular matrix, which may be modified by MSC-produced matrix metalloproteinases (MMPs) and tissue inhibitor of MMPs. (biomedcentral.com)
MSCs can be defined by their ability to adhere to tissue culture plastic, their expression of several cell surface molecular epitopes-cluster of differentiation CD73, CD90, and CD105, and others-as well as their lack of several surface markers, including CD45 [ 6 ]. (biomedcentral.com)
MSCs can be isolated from a range of tissues, but the most commonly cited sources are bone marrow (BM), adipose tissue, muscle, bone, and perinatal tissues (e.g. (biomedcentral.com)
Our work is at the stage of understanding the molecular mechanisms by which the aging of the regenerative potential of organ stem cells can be reversed by particular human embryonic factors that are capable of neutralizing the affects of aged niches on tissue regenerative capacity. (ca.gov)
Thus, the use of hESC-produced pro-regenerative factors for boosting the regenerative capacity of organ stem cells is likely to yield healthy, young tissue. (ca.gov)
Although functional organ stem cells persist in the old, tissue damage invariably overwhelms tissue repair, ultimately causing the demise of an organism. (ca.gov)
The levels of catalase (CAT) and glutathione (GSH) were measured in PC12 cells and Drosophila brain tissue. (sdbonline.org)
GSOs also mitigated the deleterious effects of GLU on the mitochondrial membrane potential and Cyt C release, thus alleviating mitochondrial dysfunction, and increased GSH levels and CAT activity in both cells and Drosophila brain tissue. (sdbonline.org)
Adult stem cells are tissue specific and have limited capacity to be expanded while pluripotent stem cells have the ability to differentiate into any cell type of the body while possessing unlimited self-renewal. (biotech-angels.com)
34] Upon damage satellite television cells become activated providing rise to proliferating myoblasts which in turn fuse to existing muscle tissue fibers or even to additional myoblasts to URMC-099 create new myofibers to correct muscle tissue harm. (biotech-angels.com)
Skeletal muscle regenerative capacity later diminishes with advancing age and decreasing numbers of SCs, and muscle tissue is steadily replaced by adipose and connective tissues[ 13 ]. (biomedcentral.com)
In small mammals brown adipose tissue (BAT) plays a predominant role in regulating energy expenditure (EE) via adaptive thermogenesis. (biomedcentral.com)
To achieve this we firstly established a mouse BAT enriched-miRNA profile by comparing miRNAs expressed in mouse BAT, white adipose tissue and skeletal muscle. (biomedcentral.com)
Discovery of active human brown adipose tissue (BAT) has renewed interest in the possibility that it may regulate metabolism and EE in humans [ 3 - 5 ]. (biomedcentral.com)
Autofluorescence imaging permits label-free cell type identification in live airway tissue and reveals the dynamic formation of airway secretory cell associated antigen passages. (elifesciences.org)

There is a longstanding belief that a given nucleus controls a defined volume of cytoplasm, so when a muscle grows (hypertrophy) or shrinks (atrophy), the number of myonuclei change accordingly. (frontiersin.org)
Recently, two independent models, one from rodents and the other from insects, have demonstrated that nuclei are not lost from skeletal muscle fibers when they undergo either atrophy or programmed cell death. (frontiersin.org)
Muscles get damaged during extreme exercise, and often have to weather changes in food availability and other environmental factors that lead to atrophy. (robert-gorter.info)
Obestatin signalling counteracts glucocorticoid-induced skeletal muscle atrophy via NEDD4/KLF15 axis. (institut-myologie.org)
Physical activity is known to prevent muscle atrophy, improve or boost metabolic activity, improve respiratory function, and positively impact muscles regarding strength, endurance, and stability. (apeironzoh.com)
We previously reported that local administration of bFGF 1 month after recurrent laryngeal nerve (RLN) paralysis compensated for atrophy of the thyroarytenoid muscle. (biomedcentral.com)
As increased neuromuscular junctions are expected to maintain myofiber volume, bFGF administration may prevent thyroarytenoid muscle atrophy in the mid to long term. (biomedcentral.com)
These treatments provide only static adjustment to the larynx, and their effects may be limited by the remaining hoarseness due to progressive atrophy of the paralyzed thyroarytenoid (TA) muscle. (biomedcentral.com)

The close association of FAPs with regenerating muscle mass fibers along with their manifestation of factors that influence myogenic differentiation such as IL-6 and IGF-1 suggests that these stromal cells may perform a supportive part in myogenic differentiation (Joe et BV-6 al. (scienceexhibitions.org)
Until recently, scientists thought this meant that nuclei - the cell control centers that build and maintain muscle fibers - are also lost to sloth. (robert-gorter.info)
Two independent studies - one in rodents and the other in insects - have demonstrated that nuclei are not lost from atrophying muscle fibers, and even remain after muscle death has been initiated. (robert-gorter.info)
RACK1 was expressed transiently in the skeletal muscle of post-natal mice, being abundant in the early phase of muscle growth and almost disappearing in adult mature fibers. (sdbonline.org)
After acute injury in muscle of both mouse and the fruit fly Drosophila melanogaster (used as alternative in vivo model) this study found that RACK1 accumulated in regenerating fibers while it declined with the progression of repair process. (sdbonline.org)
This heightened protein synthesis contributes to muscle hypertrophy, or the enlargement of muscle fibers, resulting in increased muscle mass. (2014davidsonfellows.com)
30 31 One group took the approach of transplanting sole muscle fibers which demonstrated that every myofiber including 7 or fewer satellite television cells could create over 100 new myofibers in engrafted muscles. (biotech-angels.com)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)

Still, the existence of these separate "MyoD-dependent" and "Myf5-dependent" subpopulations has been disputed, with some claiming that these MRFs are indeed coexpressed in muscle progenitor cells. (wikipedia.org)
Bober E, Franz T, Arnold HH, Gruss P, Tremblay P. Pax-3 is required for the development of limb muscles:a possible role for the migration of dermomyotomal muscle progenitor cells. (scielo.br)
2009 potentially reflecting the reduced secretion of myogenic growth element IGF-1 by these cells (Wynes and Riches 2003 While these studies demonstrate a facilitative part of innate immune cells in muscle mass regrowth after injury a direct molecular link between the innate immune system and muscle mass progenitor biology remains to be founded. (scienceexhibitions.org)
Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
Ensure high yields when passaging and culturing your primary human skeletal muscle progenitor cells (myoblasts) by using MyoCult™-SF Attachment Substrate, which is optimized to support myoblast attachment. (stemcell.com)
For more information on protocols for the derivation and expansion of human skeletal muscle progenitor cells with MyoCult™, please explore the Product Information Sheet . (stemcell.com)

Feeding with amino acids can stimulate protein synthesis in all tissues, especially in skeletal muscle as reviewed by Davis et al. (nature.com)
The expression level of the SFRP1 was highly varied across numerous pig tissues and it was down-regulated during porcine skeletal muscle development. (biomedcentral.com)
To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
What was surprising is that administering these inhibitors to the whole animal appears to reduce TGF-beta levels in the whole animal, suggesting some kind of feed-back and perhaps effects on other tissues as well as muscle. (ca.gov)
The poor performance of stem cells in an aged organ, such as skeletal muscle, is caused by the changes in regulatory pathways such as Notch, MAPK and TGF‐β, where old differentiated tissues and blood circulation inhibit the regenerative performance of organ stem cells. (ca.gov)
In clinical practice, these involve the life-long immunosuppression of the organ transplantation patients, the creation of the supporting environment for engineered tissues in the mended organ, the unsolved issues of cell survival and differentiation of the cell-based therapy, and the selection and development of vectors for gene therapy. (biomedcentral.com)
Understanding the molecular structure of titan steroids is essential to grasp how they interact with the body's cells and tissues. (2014davidsonfellows.com)
Comparison of transcriptome of skeletal muscles and other tissues between phenotypically different pig breeds has been proposed to improve the understanding of the genetic mechanisms underlying differences in growth and meat quality [ 10 ]. (biomedcentral.com)
The light must be absorbed by the endogenous chromophores of cells or tissues for actions. (biomedcentral.com)

The high redundancy of this system shows how crucial the development of skeletal muscle is to the viability of the fetus. (wikipedia.org)
The delayed growth of LBW piglets is mainly due to a slower development of skeletal muscle as the myogenic activity is usually impaired in these animals during the fetal and early postnatal period 3 , 4 . (nature.com)

Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)
My research focuses on investigating the mechanisms responsible for sarcopenia (the age-related loss of skeletal muscle mass and strength) and developing exercise interventions for the treatment/prevention of sarcopenia. (uncg.edu)
These cell lines are shared in collaboration with many international teams, either to study physiological or pathophysiological mechanisms, or to test innovative therapeutic approaches. (institut-myologie.org)
Additionally, our data in progress suggest that muscle and brain age by similar molecular mechanisms and thus, therapeutic strategies for rejuvenating muscle repair might be applicable to the restoration of neurogenesis in aged brain. (ca.gov)
The impact of titan steroids on muscle growth is multifaceted and involves various mechanisms within the body. (2014davidsonfellows.com)
However, the mechanisms of cell proliferation induced by LPLI are poorly understood. (biomedcentral.com)
then we review the studies on the molecular mechanisms of LPLI-induced proliferation since January 1999, which will serve as a reference for the researchers in this field. (biomedcentral.com)
The objective of this study was to elucidate the effects of early bFGF administration on the thyroarytenoid muscle after RLN transection and to investigate the underlying mechanisms. (biomedcentral.com)
Understanding the mechanisms controling BAT differentiation may identify potential therapeutic targets to enhance BAT-controlled metabolism and to increase EE. (biomedcentral.com)

3 20 21 Major factors underlining this poor outcome included low ability of myoblasts to migrate beyond the injection site[22 23 and poor survival of injected cells. (biotech-angels.com)
26-29] As an alternative to poorly-engrafting myoblasts much recent interest has developed around the idea of therapy with stem cells. (biotech-angels.com)
human pluripotent stem cell (hPSC)-derived myoblasts may be cultured on cultureware coated with Corning® Matrigel® hESC-Qualified Matrix. (stemcell.com)
Myoblasts cultured using MyoCult™-SF Expansion Medium can be differentiated into multinucleated myotubes using MyoCult™ Differentiation Kit (Human) . (stemcell.com)
After several rounds of proliferation, most SCs differentiate into myoblasts, which fuse to form new myofibers [ 5 ]. (biomedcentral.com)
We previously reported that local injection of single, high-dose bFGF into the paralyzed vocal fold 1 month after RLN transection compensated for an atrophied TA muscle by inducing activation of SC proliferation and increasing the number of myoblasts in the RLN paralysis model of rats [ 12 ]. (biomedcentral.com)

There his work focused on transcriptional control of hematopoietic stem cell maturation and cell fate. (ubc.ca)

Herein, the present study aimed to elucidate the effect of hypothalamic TRPV4 on high-fat diet-induced obesity (DIO) and evaluate its potential for regulating energy metabolism. (bvsalud.org)
Cell Metabolism 2013;17(2):210-24. (scielo.br)
Liver kinase B1 (LKB1) plays pivotal roles in cellular processes such as apoptosis, metabolism, and cell cycle regulation. (omicsdi.org)
Medium pH modulates matrix, mineral, and energy metabolism in cultured chick bones and osteoblast-like cells. (sciepub.com)
The manipulation of blood flow in conjunction with skeletal muscle contraction has helped us with the physiological understanding of muscle fatigue, blood pressure reflexes, and metabolism in humans. (pensummed.pro)
We observed alteration in biological functions related to extracellular matrix function and organization, cellular adhesion, muscle growth, lipid metabolism and proteolysis. (biomedcentral.com)

Yamashita D, Machigashira M, Miyamoto M, Takeuchi H, Noguchi K, Izumi Y, Ban S. "Effect of surface roughness on initial responses of osteoblast-like cells on two types of zirconia. (sciepub.com)
Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K. "Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. (sciepub.com)

This study elucidated effects of glutamine (Gln) supplementation on the cellular muscle development of LBW and normal birth weight (NBW) piglets. (nature.com)
We observed a temporarily increased intramuscular availability of free Gln that could have influenced cellular processes in skeletal muscle. (nature.com)
Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)
Roles for thrombospondin-1 signaling, mediated by specific cellular receptors or by activation of latent TGFß, have been defined in T and B lymphocytes, natural killer cells, macrophages, neutrophils, and dendritic cells. (bvsalud.org)
Musgrove E, Seaman M, Hedley D. "Relationship between cytoplasmic pH and proliferation during exponential growth and cellular quiescence. (sciepub.com)
Amphetamine, a psychostim ulant, also causes reversal and altered cellular location of the DAT which is known to be regulated by kinases, phos phatases, and Ca2 localization and association. (inhibitorkit.com)
Mostly, there is a finite amount of time that cells can survive and cumulative damage incurred from life choices can increase or decrease the speed of cellular damage. (apeironzoh.com)
Low power laser irradiation (LPLI) promotes proliferation of multiple cells, which (especially red and near infrared light) is mainly through the activation of mitochondrial respiratory chain and the initiation of cellular signaling. (biomedcentral.com)
The nuclear membrane protein SUN1 stabilizes endothelial cell-cell junctions far from the nucleus via regulation of microtubule dynamics and Rho GEF-H1 signaling, revealing long-range cellular communication important for vascular development and function. (elifesciences.org)
Cytoskeleton, U-93631 which is a network of interconnected filaments of different types, connects the cell membrane with underlying sub-cellular components. (globaltechbiz.com)
The nucleus is the largest organelle among sub-cellular components, demonstrating solid-elastic behavior [4], and it is typically stiffer than the cell itself [5]. (globaltechbiz.com)

Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)

He performed some of the first studies to identify transcription factors that regulate the gene expression and differentiation of eosinophils, which are known to play a major role in allergic and asthmatic responses. (ubc.ca)
Gene expression of Hanwoo satellite cell differentiation in longissimus dorsi and semimembranosus, Bmc Genomics, 20, 156, https://doi.org/10.1186/s12864-019-5530-7, 2019. (copernicus.org)

Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)
MicroRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7. (chinagene.cn)
30] The additional approach used a transgenic reporter mouse for Pax3 a paralog of Pax7 which allowed for the immediate isolation of Pax3+ (GFP+) muscle satellite television cells by movement cytometry. (biotech-angels.com)
31] Cells isolated from mature skeletal muscles displayed homogenous expression of Pax7 and contributed to both dietary fiber repair also to the muscle satellite television cell URMC-099 compartment subsequent their transplantation into dystrophic mice. (biotech-angels.com)
Furthermore these authors demonstrated that Pax7(+)luciferase(+) mononuclear cells could possibly be readily re-isolated offering proof for the self-renewal of the cell human population. (biotech-angels.com)
Their fate is regulated by the expression of various transcription factors, including paired box 7 (Pax7) and myogenic differentiation (MyoD) [ 6 ]. (biomedcentral.com)

MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)
To directly assess the role of beta cell lipolysis in insulin secretion and whole-body energy homeostasis, inducible beta cell-specific adipose triglyceride lipase (ATGL)-deficient (B-Atgl-KO) mice were studied under normal diet (ND) and high-fat diet (HFD) conditions. (omicsdi.org)
Although adipocyte differentiation starts early in fetal stages, the increase in adipose cell number and size is maintained along early postnatal growth and later development [ 6 ]. (biomedcentral.com)

Intro The regenerative response of skeletal muscle mass to ICAM4 injury is dependent within the quiescent populace of skeletal muscle mass stem cells termed the satellite cells which reside beneath the basal lamina of each myofiber (Brack and Rando 2012 Wang and Rudnicki 2012 Upon injury these quiescent satellite cells become triggered and undergo proliferation providing rise to myogenic progenitors (MPs) that ultimately differentiate into mature myofibers. (scienceexhibitions.org)
1 Adult Stem Cells 1.1 Satellite Cells Studies in the last decade have clearly proven that the regenerative ability of adult skeletal muscle is due to the satellite cell a quiescent stem cell population of muscle precursors located between the basal lamina and sarcolemma of each myofiber. (biotech-angels.com)
New devices have demonstrated significant results for the treatment of medical conditions, including mild-to-moderate acne vulgaris, wound healing, psoriasis, squamous cell carcinoma in situ (Bowen's disease), basal cell carcinoma, actinic keratosis, and cosmetic applications. (researchgate.net)

In vivo , the identification of macrophage phenotype is complicated since macrophages are obviously exposed to a microenvironment that is more complex respect to cell culture conditions, and they display characteristics that do not conform to the in vitro defined phenotypic categories. (hindawi.com)
Then, we comprehensively assessed the functional and histological parameters of skeletal muscle injury in mice and the phenotype shifting in macrophages through molecular biological methods and immunofluorescence analysis both in vivo and in vitro . (ijbs.com)
Since only small numbers of cells are obtainable from the patient, it requires expansion of cells in vitro . (sciepub.com)
Nevertheless, these primary cells grow slowly in vitro and lose their general characteristics during prolonged culturing period. (sciepub.com)
Results: LED produced in vitro increases of cell growth of 140-200% in mouse-derived fibroblasts, rat-derived osteoblasts, and rat-derived skeletal muscle cells, and increases in growth of 155-171% of normal human epithelial cells. (researchgate.net)
In vitro studies using infra-/red light have not shown any useful impact on wound healing by activating cells located in deeper skin layers such as fibroblasts or stem cells [2] . (researchgate.net)

The miRNA-mRNA profiles and bioinformatics study suggested that the SFRP1 gene was potentially regulated by miRNA-1/206 during porcine skeletal muscle development. (biomedcentral.com)
Dual luciferase assay and Western-blot results demonstrated that SFRP1 was a target of miR-1/206 in porcine iliac endothelial cells. (biomedcentral.com)

2010 FAPs which do not arise from your myogenic lineage are bipotential cells capable of providing rise to fibroblasts and adipocytes. (scienceexhibitions.org)
In addition, cytokines released in the process of myonecrosis recruit inflammatory cells, which release inflammatory cytokines to activate fibroblasts that lay down extracellular matrix proteins and lead to fibrosis[ 12 ]. (biomedcentral.com)

Furthermore, GSOs protected cells against GLU-induced apoptosis by reducing the expression of the mitochondrial apoptosis-associated Bcl-2 family effector proteins and protected cells from GLU-induced oxidative damage by increasing the nuclear translocation of Nrf2 and HO-1 expression. (sdbonline.org)

It functions during that time to commit myogenic precursor cells to become skeletal muscle. (wikipedia.org)

Adult mesenchymal stem cells (MSCs) represent a subject of intense experimental and biomedical interest. (biomedcentral.com)
From a research, medical, and business standpoint, mesenchymal stem cell (MSC)-based therapies are fascinating. (biomedcentral.com)
The use of mesenchymal stem cells (MSCs) as a regenerative therapy holds the promise of regrowing injured vasculature, helping DM patients live healthier and longer lives. (thno.org)

There he worked with Dr. Max D. Cooper (Howard Hughes Medical Institute, National Academy of Sciences) and his research focused on cell surface proteins expressed by preB cells that regulate B cell maturation and homing. (ubc.ca)
In addition, he identified a number of novel hematopoietic stem cell surface proteins and began analyzing their function. (ubc.ca)
His laboratory has followed two primary interests: 1) the transcription factor networks that regulate fate determination in various cells that make blood, and 2) the cell surface proteins expressed by hematopoietic stem cells that and allow them to communicate with their microenvironment. (ubc.ca)
In this regard, his lab has identified a novel family of hematopoietic cell surface proteins, called the CD34 family, and shown that these are essential for a number of developmentally important processes. (ubc.ca)
In the next reporting period we will re-confirm that the levels of candidate proteins from the 500 antibody array actually are very highly produced by hESCs and that the signals from these proteins are perceived by regenerating muscle cells. (ca.gov)
In 2010 beginning of 2011, we have approached the identification and characterization of the proteins that are produced by hESCs and have the rejuvenating and pro-regenerative activity on adult muscle. (ca.gov)
Titan steroids stimulate the cells' protein-building processes, leading to an accelerated production of muscle proteins. (2014davidsonfellows.com)
This decreases risk for various chronic diseases by eliminating damaging proteins, improving strength, and increasing muscle mass. (apeironzoh.com)
Recently, the signaling proteins involved in LPLI-induced proliferation merit special attention, some of which are regulated by mitochondrial signaling. (biomedcentral.com)
LPLI-induced cell cycle progression can be regulated by the activation or elevated expressions of cell cycle-specific proteins. (biomedcentral.com)
Recently, a large number of signaling proteins reported play an important key role in the process of LPLI-induced cell proliferation, probably due to the fact that the molecular events they are involved in are the basic response of the cells to extracellular stimuli. (biomedcentral.com)
The cell membrane is really a viscous fluid-like matter which includes several lipids, cholesterol, and inserted proteins. (globaltechbiz.com)

Macrophage activation has been described as a dynamic process: the same cell may initially induce proinflammatory and cytotoxic reactions and later may take part in the resolution of inflammation and wound healing [ 9 ]. (hindawi.com)
The IAL aims to synergise the expertise in muscle and neuromuscular diseases of French teams and the expertise in immunology and inflammation of the Brazilian teams . (institut-myologie.org)
Additionally, titan steroids can also interact with other receptors, such as glucocorticoid receptors, which play a role in regulating inflammation and immune responses. (2014davidsonfellows.com)

In addition to regulating physiological nitric oxide signaling and responses of cells to stress, studies in mice lacking thrombospondin-1 or its receptors have revealed important roles for thrombospondin-1 in regulating immune responses in infectious and autoimmune diseases and antitumor immunity. (bvsalud.org)
BFRT exercise alters acute physiological stressors such as local muscle oxygen availability and vascular shear stress which may lead to adaptations that are not easily attained with conventional training. (pensummed.pro)
in these cells physiological lev els of E2 and low levels of xenoestrogens can rapidly reverse actions of the DAT. (inhibitorkit.com)
Cell proliferation is a very important physiological effect for low power laser irradiation (LPLI) used in clinical practice. (biomedcentral.com)

He has delineated the function of these molecules in diverse set of biological processes including: 1) gut and kidney formation, 2) vascular permeability, 3) mucosal inflammatory disease, 4) stem cell homing and migration, and 5) epithelial tumor progression. (ubc.ca)
Immune cells that infiltrate lesions are important for atherosclerosis progression and immunotherapies. (biomedcentral.com)
A long-term multi-organ co-culture system coupled with quantitative imaging to visualize blood cell cycle progression, analyze polarized blood cell mitosis, and track blood cell differentiation kinetics during Drosophila hematopoiesis in homeostatic condition and following infection. (elifesciences.org)
Introduction Cell mechanics has proved to be a widely used label-free biomarker to discern phenotypes, detect pathologies and more importantly, monitor existence or progression of a disease [1C3]. (globaltechbiz.com)
Secondly, they can improve our understanding of the adjustments that occur during disease progression which, in turn, might uncover reasons for corresponding alterations occurring in cell mechanics [10, 11]. (globaltechbiz.com)

Finally, our preliminary data suggest that the ESC-ovarian tumor model has human tumor vascular cells. (nih.gov)
Tumor vascular cells are critical for the growth of tumor stem cells, which reside within the vascular niche. (nih.gov)
We hypothesize that the ESC ovarian tumor model, with human vascular cells, will provide an ideal microenvironment to support human stem cell growth. (nih.gov)
Vascular endothelial growth factor (VEGF) is important for the maintenance of the skeletal muscle capillaries. (uncg.edu)
Recently, we observed that muscle capillarization, vascular endothelial growth factor (VEGF) protein, and the VEGF mRNA response to acute exercise were lower in aged compared with young women. (uncg.edu)
2017. Potency of human cardiosphere-derived cells from patients with ischemic heart disease is associated with robust vascular supportive ability . (cardiff.ac.uk)

microRNAs in skeletal muscle differentiation and disease. (chinagene.cn)

These receptors are primarily found in skeletal muscle cells, leading to an increase in protein synthesis and promoting muscle growth. (2014davidsonfellows.com)

Therefore, in collaboration with W. Wright, we have developed an approach for the immortalisation of human muscle precursors that allows them to retain their ability to differentiate: this has enabled the generation of immortal cell lines derived from patient cells. (institut-myologie.org)
These cells have the ability to self-renew and to differentiate into specialized cell types and can be primarily classified as adult and pluripotent stem cells which differ significantly in regard to their differentiation potential and expansion capability. (biotech-angels.com)

Thrombospondin-1 is a secreted matricellular glycoprotein that modulates cell behavior by interacting with components of the extracellular matrix and with several cell surface receptors. (bvsalud.org)
Its presence in the extracellular matrix is induced by injuries that cause thrombospondin-1 release from platelets and conditions including hyperglycemia, ischemia, and aging that stimulate its expression by many cell types. (bvsalud.org)
Heparan sulfate proteoglycans (HSPGs) are present on the cell surface and in the extracellular matrix in all metazoans. (scielo.br)
When activated by injury, satellite cells reenter the cell cycle and proliferate in response to extracellular growth factors ( Relaix & Zammit, 2012 Relaix F, Zammit PS. (scielo.br)
The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
These MSC influences can range from relatively rare activities that require cell contact, such as mitochondrial transfer and cell fusion, to relatively common paracrine MSC actions through extracellular microvesicles or secreted factors. (biomedcentral.com)

If successful, this will create a murine tumor model that nearly completely reproduces the human tumor microenvironment with human tumor stroma, vessels and tumor stem cells. (nih.gov)

Moreover, miRNAs play a modulatory role in the development and growth of skeletal muscles [ 22 ]. (biomedcentral.com)
Three miRNAs, miRNA-1, −133 and −206, are specifically expressed in muscle and are considered to be myomiRs [ 23 , 24 ]. (biomedcentral.com)
It's been suggested that miRNAs regulate up to 50% of all mRNAs in the human genome. (genengnews.com)

Differentiation begins (regulated by other genes) after this initial proliferation. (wikipedia.org)
Expression of muscle growth related genes was quantified with qPCR. (nature.com)
The objective of this work was the evaluation of muscle transcriptome profile in piglets of both genetic types, in order to identify genes, pathways and regulatory factors responsible for their phenotypic differences. (biomedcentral.com)
Candidate genes with known effects on muscle growth were found among the DE genes upregulated in DU×IB. (biomedcentral.com)

We have previously examined a well characterized this research non transfected neuronal cell culture model that expresses three known mERs mER, mER, and GPR30. (inhibitorkit.com)

Some evidence shows that Myf5 and MyoD are responsible for the development of separate muscle lineages, and are not expressed concurrently in the same cell. (wikipedia.org)

Most notably, while Myf5 is activated by Sonic hedgehog in the epaxial lineage, it is instead directly activated by the transcription factor Pax3 in hypaxial cells. (wikipedia.org)
While the product of this gene is capable of directing cells towards the skeletal muscle lineage, it is not absolutely required for this process. (wikipedia.org)
Role of glycosphingolipid SSEA-3 and FGF2 in the stemness and lineage commitment of multilineage differentiating stress enduring (MUSE) cells. (nih.gov)
In Drosophila with depleted RACK1 in all muscle cells or, specifically, in SC lineage resulted in a delayed recovery of skeletal muscle after physical damage as well as the low presence of active SC in the wound area. (sdbonline.org)

2012 whereas paracrine actions of IL-6 and insulin-like growth factors (IGFs) have been implicated in the differentiation of MPs into mature myotubes (Bodine et al. (scienceexhibitions.org)
The dynamics of RACK1 levels in isolated adult SC of mice, i.e., progressively high during differentiation and low compared to proliferating conditions, and RACK1 silencing indicated that RACK1 promotes both the formation of myotubes and the accretion of nascent myotubes. (sdbonline.org)

While acknowledging progress in the other areas mentioned, this work will focus on the current debates concerning sourcing, MSC alterations of angiogenesis, cell differentiation/stimulation, and strategies to improve MSC differentiation. (biomedcentral.com)

This review concentrates on the research progress of the non-canonical Hippo pathway in regulating innate immune cell anti-infection responses, maintaining redox homeostasis, responding to microenvironmental stiffness, and T-cell differentiation. (bvsalud.org)

35-39] Meanwhile a little subset of satellite television cells will not undergo differentiation but wthhold the ability to go back to a quiescent state and therefore preserve the satellite television cell pool. (biotech-angels.com)

One challenge with characterizing tumor stem cells has been finding appropriate conditions for in vivo growth. (nih.gov)
We therefore propose (3) to isolate ovarian tumor stem cells and grow them in vivo using the ESC ovarian cancer model. (nih.gov)
Cell invasion is a crucial step of tumor metastasis , finding new regulators of which offers potential drug targets for cancer therapy. (sdbonline.org)
Hepatocyte growth factor receptor (c-Met), a member of tyrosine protein kinase receptors (TPKR), is phosphorylated during LPLI-induced proliferation, but tumor necrosis factor alpha (TNF-alpha) receptor has not been affected. (biomedcentral.com)

At 8 weeks of age, these mice were injected with tamoxifen to induce deletion of beta cell-specific Atgl (also known as Pnpla2), and the mice were fed an ND or HFD. (omicsdi.org)
We reported that LIPUS therapy at intensity of 60mW/cm 2 exhibited the most significant differences in functional recovery of contusion-injured muscle in mice. (ijbs.com)
Mice with muscle-speci?c inactive AMPK (AMPK DN) were used to invest. (uncg.edu)
31] Down the road Sacco and colleagues proven that intra-muscular transplantation of an individual luciferase-expressing muscle stem cell isolated from Myf5 reporter mice led to intensive proliferation and contribution to muscle materials. (biotech-angels.com)
In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)

Through gene knockout studies he has shown that these molecules act as a type of molecular "Teflon" to make cells more mobile and invasive and also facilitate chemotaxis. (ubc.ca)
Nature Reviews Molecular Cell Biology 2012;13(2):127-33. (scielo.br)
Molecular motors lie at the heart of biological processes from DNA replication to cell migration. (nih.gov)
The molecular structure of titan steroids allows them to bind to specific receptors inside cells, including androgen receptors. (2014davidsonfellows.com)
These changes take place on the molecular level impacting properties of specific the different parts of cell inner structure, but resulting in alterations in mechanical properties of the complete cell ultimately. (globaltechbiz.com)

Our team has demonstrated some important differences between man and mouse, in the regulation of the proliferation of myogenic precursors. (institut-myologie.org)

The SFRPs inhibit Wnt receptor binding to down-regulate pathway signaling during development [ 8 ]. (biomedcentral.com)
Transient receptor potential vanilloid 4 (TRPV4) is involved in regulating osmotic pressure, temperature and mechanical force transmission, but little is known about its role in obesity. (bvsalud.org)
KIT is a receptor tyrosine kinase type III, which binds to stem cell factor. (inhibitorkit.com)
The thymic selection of the human T-cell receptor repertoire releases polyspecific receptors with the ability to recognize and respond to peptides from unrelated viruses. (elifesciences.org)

The expression level of the SFRP1 was significantly higher in the embryonic skeletal compared with postnatal skeletal muscle, whereas miR-206 showed the inverse pattern of expression. (biomedcentral.com)
Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)
Panobinostat therapy significantly repressed mRNA for DNMT1 and DNMT3a in the two cell lines while no modifications had been observed in DNMT3b levels. (thrombininhibitors.com)
However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
The cross-sectional thyroarytenoid muscle area was significantly larger in the high-dose group than in the saline and low-dose groups on days 28 and 56. (biomedcentral.com)
Immunohistochemistry indicated that bFGF significantly increased the number of satellite cells in the thyroarytenoid muscle up to day 14 and that of neuromuscular junctions on days 28 and 56. (biomedcentral.com)

In addition, we showed that during SMSC differentiation, the downregulated levels of EZH2 mRNA and protein were accompanied by increasing miR-101a expression via qRT-PCR and Western blot. (chinagene.cn)
Panobinostat treatment drastically repressed mRNA for DNMT1 and DNMT3a in the two cell lines whilst no adjustments have been observed in DNMT3b levels. (ilreceptor.com)

With this context of injury and repair a number of factors have been recognized that promote proliferation and differentiation of MPs (Kuang et al. (scienceexhibitions.org)

Depletion of macrophages in vivo eliminated the therapeutic effects of LIPUS, indicating that improvement in muscle function was the result of M2-shifted macrophage polarization. (ijbs.com)
Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)

Additionally, titan steroids improve the efficiency of muscle cells' energy production. (2014davidsonfellows.com)

When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)

These and other data argue against the current interpretation of the myonuclear domain hypothesis and suggest that once a nucleus has been acquired by a muscle fiber it persists. (frontiersin.org)

Incomplete migration of enteric neural crest-derived cells (ENCDC) leads to Hirschsprung disease, a congenital disorder characterized by the absence of enteric ganglia along variable lengths of the colorectum. (bvsalud.org)
Firstly, loss of dGLYAT suppressed scrib depletion- or Egr overexpression-induced JNK pathway activation and invasive cell migration. (sdbonline.org)
Commensurate with the known truth that HuR improved the great quantity of adhesion substances VLA-4 on Th17 cells, knockout of HuR impaired splenic Th17 cell migration towards the central anxious program and abolished the condition. (globaltechbiz.com)

Yamaguchi DT, Huang JT, Ma D. "Regulation of gap junction intracellular communication by pH in MC3T3-E1 osteoblastic cells. (sciepub.com)
Recent mechanistic insights into the trophic activities of these cells point to ultimate regulation by nitric oxide, nuclear factor-kB, and indoleamine, among other signaling pathways. (biomedcentral.com)
Crist CG, Montarras D, Pallafacchina G, Rocancourt D, Cumano A, Conway SJ, Buckingham M. Muscle stem cell behavior is modified by microRNA-27 regulation of Pax3 expression. (chinagene.cn)

They have proinflammatory functions: they produce effector molecules (reactive oxygen and nitrogen intermediates) and inflammatory cytokines (IL-1 β , TNF α , and IL-6) and participate as inducer and effector cells in polarized Th1 responses. (hindawi.com)

Meat science and muscle biology symposium: stem cell niche and postnatal muscle growth, J. Anim. (copernicus.org)

Despite only being present for a few days during embryonic development, Myf5 is still expressed in certain adult cells. (wikipedia.org)

Cell damage was assessed using MTT assays, and the lactate dehydrogenase (LDH) release rate and flow cytometry were used to detect the accumulation of reactive oxygen species (ROS) and mitochondrial membrane potential respectively. (sdbonline.org)

Anatomy64

Organisms12

Diseases10

Chemicals and Drugs75

Analytical, Diagnostic and Therapeutic Techniques and Equipment34

Phenomena and Processes58

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Regeneration31

Embryonic stem3

Myogenesis7

Tissue31

Atrophy8

Fibers8

Progenitor6

Tissues9

Development of skeletal muscle2

Mechanisms9

Myoblasts6

Transcriptional1

Metabolism6

Osteoblast-like cells2

Cellular11

Regenerative capacity1

Gene expression2

Pax76

Adipose3

Basal3

Vitro6

Porcine2

Fibroblasts2

Apoptosis1

Precursor cells1

Mesenchymal3

Proteins12

Inflammation3

Physiological4

Progression5

Vascular6

MicroRNAs1

Protein synthesis1

Differentiate2

Extracellular6

Microenvironment1

MiRNAs3

Genes4

Neuronal1

Myod1

Lineage4

Myotubes2

Stimulation1

Innate immune1

Undergo1

Tumor4

Mice6

Molecular5

Myogenic precursors1

Receptor4

Significantly6

MRNA2

Kuang1

Therapeutic2

Additionally1

Myofibers2

Hypothesis1

Migration3

Regulation3

Effector cells1

Postnatal muscle growth1

Adult cells1

Mitochondrial1