AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsTechnology, Industry, AgricultureInformation Science
Cell DifferentiationStem CellsEmbryonic Stem CellsHematopoietic Stem CellsCells, CulturedCell LineageMesenchymal Stromal CellsAdult Stem CellsStem Cell TransplantationPluripotent Stem CellsNeural Stem CellsGene Expression Regulation, DevelopmentalSignal TransductionCell LineCell ProliferationTranscription FactorsGene Expression RegulationMice, Inbred C57BLInduced Pluripotent Stem CellsMultipotent Stem CellsCell Culture TechniquesRNA, MessengerEmbryo, MammalianHomeodomain ProteinsReverse Transcriptase Polymerase Chain ReactionCell DivisionTretinoinOsteogenesisBone Marrow CellsStem Cell NicheOctamer Transcription Factor-3Embryoid BodiesPhenotypeGene Expression ProfilingAdipogenesisHematopoietic Stem Cell TransplantationMice, KnockoutMolecular Sequence DataDNA-Binding ProteinsMice, TransgenicGene ExpressionFlow CytometryImmunohistochemistryBase SequenceModels, BiologicalNeuronsHematopoiesisOsteoblastsRegenerationStem Cell FactorMesenchymal Stem Cell TransplantationEndodermTissue EngineeringBasic Helix-Loop-Helix Transcription FactorsMesodermSex DifferentiationAntigens, DifferentiationTranscription, GeneticNeoplastic Stem CellsCoculture TechniquesBiological MarkersTime FactorsRepressor ProteinsPromoter Regions, GeneticNeurogenesisBone Morphogenetic Protein 2Epigenesis, GeneticReceptors, NotchChondrogenesisSOXB1 Transcription FactorsBone Morphogenetic ProteinsCell SurvivalDown-RegulationBlotting, WesternCell SeparationTransfectionMuscle DevelopmentCell CycleMutationMicroRNAsGerm CellsAdipocytesApoptosisHemangioblastsNuclear ProteinsLeukemia Inhibitory FactorGreen Fluorescent ProteinsUp-RegulationRegenerative MedicineFluorescent Antibody TechniqueFetal Stem CellsOligonucleotide Array Sequence AnalysisDNA PrimersSpermatogoniaNerve Tissue ProteinsCell ShapeAmino Acid SequenceTrans-ActivatorsTumor Cells, CulturedIn Situ Hybridization
VitroNeuronalMouse embryonicESCsRole during embryonic deveProliferationEarly embryonic developmentRegenerativeInduceEmbryoid BodiesExtra embryonicGene expressionHematopoietic stemRegulatesSoluble growth factorsGenesIPSCsCardiomyocytesFetalPluripotencyMesenchymalLethalityCommitsNeural cellsTransgenicGermAdhesionPotentialityHumanTissueLineagesProgenitor cellBiologyLineage cellsMyogenicPathwaysRetinoic AcidBone marrowPancreaticVivoPopulationsFateHematopoiesisDifferentiateCellularTissuesInductionProteinMicroenvironmentAntigenEpithelialFACSNeuronsEstablishmentMolecularMorphogenesisExtracellular matrixAffectsTumors
Vitro22
  • At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
  • We detect alterations of protein properties in numerous cellular pathways and components including ribosome biogenesis and demonstrate that modulation of ribosome maturation through SBDS protein can be helpful for manipulating cell stemness in vitro. (nature.com)
  • Using our integrative proteomics approach and the web-based tool, we uncover a molecular basis for the uncoupling of robust transcription from parsimonious translation in stem cells and propose a method for maintaining pluripotency in vitro. (nature.com)
  • We portrayed the Notch system in embryonic stem cell (ESC)-derived embryoid bodies (EBs) differentiating under the standard protocols used to assess yolk sac (YS) hematopoiesis in vitro. (karger.com)
  • Widespread Notch1 activation was evident at days 2-4 of EB differentiation, the time window of hemangioblast generation in this in vitro system. (karger.com)
  • Hematopoietic Stem Cell and Leukaemic Stem Cell environmental stress responses (In vitro biomimicry of hypoxia, normoxia, hypothermia, hyperthermia, hypoglycemia and hyperglycemia): Effect on cellular growth, cell cycle distribution, apoptosis and cellular metabolism. (imperial.ac.uk)
  • A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
  • By using human lungs and cells, this new system provides a novel way to culture cells in vitro to investigate matrix contributions to lung pathology in a disease-specific manner. (atsjournals.org)
  • On the other hand, even if 3D aggregate culture methods are able to recapitulate in vitro the cell-extracellular matrix interactions properly observed in vivo, and the synthetic/natural matrix and scaffolds have biochemical and mechanical properties, in order to mimic the native extracellular matrix, both of these systems possesses some limitations and some methodological improvements are needed. (razavihospital.ir)
  • Results: The processes by which re-aggregated adult single cell types or adult and embryonic explanted tissues are able to recapitulate embryogenesis in vitro, when cultured in adhesion or embedded in 3D gels, is not surprising and is clearly under the control of a reminiscent cellular memory which recapitulates early developmental stages. (razavihospital.ir)
  • Moscona A. The Development in Vitro of Chimeric Aggregates of Dissociated Embryonic Chick and Mouse Cells. (razavihospital.ir)
  • Kurosawa H. Methods for inducing embryoid body formation: in vitro differentiation system of embryonic stem cells. (razavihospital.ir)
  • Culture and differentiation of preadipocytes in two-dimensional and three-dimensional in vitro systems. (razavihospital.ir)
  • We discuss what is known about the distinct metabolic states captured in vitro by the 2-cell-like, naïve, blastocyst-like, formative, and primed states of pluripotency. (conditionmed.org)
  • 2019). Akin to the dynamic nutrient requirements of the developing embryo, discrete in vitro cell states have distinct metabolic profiles (Zhou et al. (conditionmed.org)
  • It is only through understanding embryonic metabolism and development that we can derive and maintain different in vitro stem cell states for disease modeling and therapies. (conditionmed.org)
  • Our results suggest a fundamental role for mechanosensing in mammalian development and illustrate that the mechanical environment should be taken into consideration when engineering implantable scaffolds or when producing therapeutically relevant cell populations in vitro. (ox.ac.uk)
  • Identification of genes involved in in vitro differentiation induction of embryonic stem cells (ESCs) into hematopoietic stem cells (HSCs) has been challenged during last decade. (ebiotrade.com)
  • Transduction of Lhx2 into ESC-derived mesodermal cells resulted in robust differentiation of c-Kit+/Sca-1+/Lineage- (KSL) cells in vitro. (ebiotrade.com)
  • Transduction of Lhx2 into induced pluripotent stem cells (iPSCs) was also effective in generating KSL cells in vitro, as well as HSC-like activities in vivo. (ebiotrade.com)
  • In vitro experiments using NV virus-like particles (VLPs) directly showed NV VLP attachment to HBGA, resulting in VLP internalization into the cell ( 19 ). (cdc.gov)
  • From these critical studies, putative NV receptors were identified, and thus it was hypothesized that a successful in vitro cell culture system would most likely possess these receptors to support NV replication. (cdc.gov)
Neuronal8
  • Ngns (class II bHLH transcription factors) are known as pro-neural factors as they are necessary to initiate differentiation of NSPCs and are important to specify a neuronal subtype[3]. (bartleby.com)
  • Thoma et al observed that Ngn2 alone is sufficient to induce neuronal differentiation in embryonic stem cells. (bartleby.com)
  • In this study they used murine embryonic stem cells and transfected with Ngn2 expression and after five days post transfection they observed that cells expressed Tuj1 and MAP2 (both are neuronal markers). (bartleby.com)
  • TWS 119 induces neuronal differentiation in pluripotent murine embryonal carcinoma cells and embryonic stem cells at 400 nM. (tocris.com)
  • The housekeeping gene hypoxanthine guanine phosphoribosyltransferase (HPRT) regulates multiple developmental and metabolic pathways of murine embryonic stem cell neuronal differentiation. (ucsd.edu)
  • The resulting differentiated populations were analysed for transcript expression of a range of multi-lineage markers i e neuronal, myogenic, exocrine and endocrine pancreas, extra-embryonic and apoptotic markers. (dcu.ie)
  • Applying 2-stage regulated differentiation protocols developedduring the course of the study, we demonstrated the derivation of an intermediate multi-potential population (RAJEBs) from undifferentiated ES cells that preferentially gave rise to pancreatic endocrine insulin-expressing cell types in the presence of SB and neuronal-like cell types in the presence of AA or BTC. (dcu.ie)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
Mouse embryonic5
ESCs15
  • Stem cells, including embryonic stem cells (ESCs) and adult stem cells, have the potential of self-renewal and differentiation into multiple cell types and therefore hold great promise in regenerative medicine [ 11 - 13 ]. (biomedcentral.com)
  • Induced pluripotent stem cells (iPSCs) are a type of newly defined stem cell with properties similar to those of ESCs in terms of self-renewal and differentiation. (biomedcentral.com)
  • According to their potentiality, embryonic stem cells (ESCs) are classified as totipotent and pluripotent, both of them can differentiate into the three germ lines. (biomedcentral.com)
  • ESCs are obtained from inner cell mass, characterized by unlimited self-renewal and pluripotentiality, which allow them to differentiate into various specialized cells types in a morphologically and functionally way, characteristics that make them attractive for developing differentiated kidney cells (DKCs) [ 9 ]. (biomedcentral.com)
  • The team, led by Associate Professor Tomohide Takaya from the Department of Agricultural and Life Sciences at Shinshu University, recently discovered that an anti-nucleolin aptamer, myogenetic oligodeoxynucleotide iSN04, induced myocardial differentiation in embryonic stem cells (ESCs). (shinshu-u.ac.jp)
  • Using various assays like RNA sequencing, cell staining and imaging, and molecular interaction and pathway analysis, the researchers investigated iSN04's effect on murine ESCs and iPSCs. (shinshu-u.ac.jp)
  • Chemical factors such as soluble growth factors and insoluble components of the extracellular matrix are known to affect the differentiation of murine ESCs. (ox.ac.uk)
  • By growing ESCs on flexible polydimethylsiloxane substrates with varying stiffness, we tested the hypothesis that substrate stiffness can influence ESC differentiation. (ox.ac.uk)
  • Finally, we demonstrated that osteogenic differentiation of ESCs was enhanced on stiff substrates compared to soft substrates, illustrating that the mechanical environment can play a role in both early and terminal ESC differentiation. (ox.ac.uk)
  • Here, we show that HSC-like cells were efficiently induced from mouse ESCs by enforced expression of Lhx2, a LIM-homeobox transcription factor. (ebiotrade.com)
  • MicroRNA-214(miR-214) has been recently reported to regulate angiogenesis and embryonic stem cells (ESCs) differentiation. (oncotarget.com)
  • However, very little is known about its functional role in vascular smooth muscle cells (VSMCs) differentiation from ESCs. (oncotarget.com)
  • Murine ESCs were seeded on collagen-coated flasks and cultured in differentiation medium for 2 to 8 days to allow VSMCs differentiation. (oncotarget.com)
  • Importantly, miR-214 overexpression in ESCs promoted VSMCs differentiation in vivo . (oncotarget.com)
  • Mechanistically, our data showed that miR-214 regulated VSMCs gene expression during VSMCs differentiation from ESCs through suppression of QKI. (oncotarget.com)
Role during embryonic deve2
  • Whereas vitamin A is best known for its critical role during embryonic development and visual cycle (documented by 3 Nobel prizes), its post-natal activities, including in neuroprotection, modulation of neurotransmission and synaptic plasticity only start being discovered and understood. (igbmc.fr)
  • however, its role during embryonic development remains poorly understood. (bvsalud.org)
Proliferation10
  • Scientists have discovered that fucoidans are a source of necessary components of cell-surface molecules that facilitate cell-to-cell signaling, the core regulatory process responsible for everything from immunity and cardiovascular function to healthy cellular proliferation. (lifeextension.com)
  • TWS 119 enhances the proliferation and survival of γδ T-cells, and induces differentiation of CD8+ T cells towards a stem cell-like memory T cell phenotype at 2.5 μM. (tocris.com)
  • Platelet-rich fibrin (PRF), commonly known as a second-generation PC, was shown to have a high capacity to improve wound healing and tissue repair owing to the gradual release of growth factors during its slow degradation along with its intrinsic fibrin scaffolding, which offers a unique three-dimensional (3-D) microstructure for promoting proliferation and differentiation of recruited cells ( 12 , 13 ). (spandidos-publications.com)
  • The transplantation of iPSC-derived RPCs decreased the levels of biomarkers indicative of renal injury and attenuated the necrosis and apoptosis of renal tissues, but resulted in the up-regulation of renal tubules formation, cell proliferation, and the expression of pro-renal factors. (biomedcentral.com)
  • Our results revealed that iPSC-derived RPCs can protect AKI rat from renal function impairment and severe tubular injury by up-regulating the renal tubules formation, promoting cell proliferation, reducing apoptosis, and regulating the microenvironment in the injured kidney. (biomedcentral.com)
  • In response to acute injury, the adult kidney shows some level of regeneration characterized by the proliferation of the surviving cells and the replacement of the necrotic tubular cells with functional tubular epithelium [ 3 ]. (biomedcentral.com)
  • Since mesenchymal stem cells (MSCs) have effective immune-modulatory features, inhibit T-lymphocyte proliferation, and have a high regenerative capacity, they can play a vital role in the reconstruction of injured tissues, including bronchioles, in pulmonary diseases. (biomedcentral.com)
  • This loss in the glucose stimulated insulin secretion (GSIS) phenotype correlates with changes in morphology, increased proliferation and increased alkaline phosphatase (AP) activity, suggesting that the loss of GSIS may be due to de-differentiation of the high passage MIN6 cells. (dcu.ie)
  • First, we are interested in Epstein Barr Virus-mediated mechanisms of immune evasion with particular emphasis on pathways that promote survival and proliferation of EBV B cell lymphomas, the characterization of the human T cell and NK cell response to EBV and the identification of novel therapeutics for treatment of EBV B cell lymphomas. (stanford.edu)
  • FGF-10 immunostaining could be related to cell proliferation in epithelium and cell differentiation in epithelium and ectomesenchyme. (bvsalud.org)
Early embryonic development2
Regenerative13
Induce4
  • The consensus of researchers studying RILI is that ionizing radiation could induce damage to epithelial cells and endothelial cells, dysfunction of the blood-air barrier, and increase vascular permeability. (biomedcentral.com)
  • Treatment of these aggregates with retinoic acid (RA) was previously shown to induce endodermal differentiation We exposed RA_EBs to secondary treatment with agents associated with pancreatic endocrine differentiation, e g Activin A (AA), Sodium Butyrate (SB) and Betacellulin (BTC), with the aim of developing a novel protocol for the regulated derivation of insulin-expressing cell types. (dcu.ie)
  • Most notably, the Cas enzyme can be expressed in human cells and, by providing a human DNA-directed gRNA, induce a highly specific DNA double strand break that cannot be repaired error-free, leading to insertion and deletion (InDel) mutations. (biochem2.com)
  • Similarly, GATA-1 has been shown to induce lineage switching expression values even if, for simplicity, we assume only ``on'' of committed cells in hematopoiesis, first in cell lines (Kulessa and ``off'' states for each gene. (lu.se)
Embryoid Bodies2
Extra embryonic1
Gene expression4
  • 2007 ) Polony analysis of gene expression in ES cells and blastocysts. (neurotree.org)
  • Histone modifications and chromatin-associated protein complexes are crucially involved in the control of gene expression, supervising cell fate decisions and differentiation. (researchgate.net)
  • To investigate this hypothesis further, gene expression differences between low passage and high passage MIN6 cells were investigated by microarray and real-time PCR (qPCR) analysis. (dcu.ie)
  • In reality, gene somatic cells to a pluripotent cell state by a handful of transcrip- expression is graded, making the potential gene expression tion factors (Takahashi and Yamanaka, 2006). (lu.se)
Hematopoietic stem1
Regulates1
Soluble growth factors1
Genes8
  • Background: Bivalent chromatin domains consisting of the activating histone 3 lysine 4 trimethylation (H3K4me3) and repressive histone 3 lysine 27 trimethylation (H3K27me3) histone modifications are enriched at developmental genes that are repressed in embryonic stem cells but active during differentiation. (researchgate.net)
  • Here we show that bivalent domains and chromosome architecture for bivalent genes are dynamically regulated during the cell cycle in human pluripotent cells. (researchgate.net)
  • Central to this is the transient increase in H3K4-trimethylation at developmental genes during G1, thereby creating a 'window of opportunity' for cell-fate specification. (researchgate.net)
  • Genes Cells. (razavihospital.ir)
  • However, when these pluripotent stem cells were treated after experiencing differentiating conditions for five days, specific marker genes were upregulated, and the cells committed to forming beating cardiomyocytes. (shinshu-u.ac.jp)
  • Similarly, several genes expressed in the primitive streak during gastrulation and implicated in early mesendoderm differentiation, such as Brachyury, Mixl1 and Eomes, were upregulated in cell cultures on stiffer compared to softer substrates. (ox.ac.uk)
  • In the present study, we assessed the hypothesis that miR-214 and its target genes play an important role in VSMCs differentiation. (oncotarget.com)
  • explosion further, consider that a fictitious small genome with 2002) More recently and more dramatically, the potential for 260 genes would host the same number of combinations as cell state conversions is exemplified by the reprogramming of the number of atoms in the visible universe! (lu.se)
IPSCs5
Cardiomyocytes1
Fetal3
  • My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
  • Shih CC, Forman SJ, Chu P, Slovak M. Human embryonic stem cells are prone to generate primitive, undifferentiated tumors in engrafted human fetal tissues in severe combined immunodeficient mice. (razavihospital.ir)
  • 1983) and the multipotent progenitor cells from fetal disease (Bjorklund and Lindvall, 2000). (lu.se)
Pluripotency5
  • To get a new insight into pluripotency, we employed a plurifaceted experimental design combining expression proteomics with the proteome-wide integral solubility alteration (PISA) assay 7 to compare pluripotent cells with their isogenic progenies and parental cells as well as with allogeneic cells. (nature.com)
  • Molecular cues for pluripotency, genetic reprogramming of adult stem cells, and widely multipotent adult cells. (razavihospital.ir)
  • We explore the recently described metabolic surge event that occurs as pluripotency is lost and stem cells commit to differentiate. (conditionmed.org)
  • To establish protocols for the safe and efficient differentiation of healthy cells for therapies, we must develop a better understanding of the dynamic continuum of metabolic states that span pluripotency and differentiation, and how to influence them. (conditionmed.org)
  • The reporter cell line shows the cellular endogenous activity of pluripotency gene Oct3/4 with green fluorescent protein (GFP) while also reporting mesoderm-specific gene, Brachyury, activity with DsRed fluorescent protein. (siu.edu)
Mesenchymal4
  • Cell-based therapy with mesenchymal stem cells or renal progenitor cells (RPCs) resident in the kidney has appeared to be an effective strategy for the treatment of AKI. (biomedcentral.com)
  • Over the last few decades numerous studies have reported the beneficial effects of mesenchymal stem cells (MSCs) on tissue repair and regeneration. (biomedcentral.com)
  • The mechanisms of radiation-induced lung injury (RILI) and the mechanisms by which mesenchymal stem cells (MSCs) alleviate it. (biomedcentral.com)
  • Alveolar epithelial cells participate in the pathogenesis of fibrosis, producing pro-inflammatory mediators, and undergoing epithelial-to-mesenchymal transition (EMT). (biomedcentral.com)
Lethality2
  • Targeted inactivation of p12, CDK2 associating protein 1, leads to early embryonic lethality. (nih.gov)
  • Disruption of murine leads to embryonic lethality seen as a a major lack of bloodstream cells and reductions in hematopoietic precursors (22, 23). (researchhunt.com)
Commits3
Neural cells1
  • Lorberbaum DS, Gottlieb D. ( 2011 ) Regulated expression of transgenes in embryonic stem cell-derived neural cells. (neurotree.org)
Transgenic1
  • Expression of cell-cycle regulator CDK2-associating protein 1 (p12CDK2AP1) in transgenic mice induces testicular and ovarian atrophy in vivo. (nih.gov)
Germ3
  • The blastocyst comprises the inner cell mass (ICM), which gives rise to the three primary germ layers and consequently the fetus, and the trophectoderm (TE), which gives rise to the extraembryonic and placental tissue. (conditionmed.org)
  • Sertoli cells and germ cells. (researchhunt.com)
  • Here in this work, the synergistic effect of chemical and mechanical signaling in driving cells towards mesoderm germ-layer was investigated. (siu.edu)
Adhesion3
  • Plakoglobin is a component of both the adherens junctions and desmosomes, and therefore plays a vital role in the regulation of cell-cell adhesion. (oncotarget.com)
  • Similar to β-catenin, plakoglobin is capable of participating in cell signaling in addition to its role in cell-cell adhesion. (oncotarget.com)
  • The structural integrity of these tissues requires extensive cell-cell adhesion and interactions mediated by the adhesive junctional complexes consisting of the adherens junctions and desmosomes [ 2 - 5 ]. (oncotarget.com)
Potentiality2
  • Embryonic or adult SCs by definition are characterized by their self-renewal and potentiality, peculiarities that allow them to give rise to more SCs and differentiate into various cell lineages under appropriate conditions. (biomedcentral.com)
  • Potentiality of embryonic stem cells: an ethical problem even with alternative stem cell sources. (razavihospital.ir)
Human18
  • 2019 ) Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding. (neurotree.org)
  • We apply our method to study differences between human pluripotent stem cells and several cell types including their parental cell line and differentiated progeny. (nature.com)
  • Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)
  • Ganglioside GQ1b promotes the differentiation of human neuroblastoma cells, murine embryonic stem cells via the activation of the ERK1/2 pathway, and murine keratinocytes via phosphoinositide turnover. (enzolifesciences.com)
  • Teratoma formation by human embryonic stem cells is site dependent and enhanced by the presence of Matrigel. (razavihospital.ir)
  • Purinergic signaling in human pluripotent stem cells is regulated by the housekeeping gene encoding hypoxanthine guanine phosphoribosyltransferase. (ucsd.edu)
  • Here, we used ChIP followed by high-throughput sequencing (ChIP-seq) to analyse genomic occupancy of CFP1 in two human haematopoietic cell types. (biomedcentral.com)
  • Future studies with human pluripotent stem cells may transduce cells towards mesoderm and finally towards cardiac fate. (siu.edu)
  • Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model. (go.jp)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • Immunohisto- have been used for positive selection of NSCs from em- chemistry on human embryonic central nervous system bryonic mice (Nagato et al. (lu.se)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • This strategy provides a useful tool for visualization and identification of neural progeny derived from human pluripotent stem cells. (lu.se)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Given this, human pluripotent stem cells, such as human embryonic stem cells (hESCs) and induced pluripo- tent stem cells (hiPSCs), have become a unique cell source for study- ing early human brain development. (lu.se)
  • However, current protocols for deriving neurons from human pluripotent stem cells give rise to heterogeneous cell populations both in regard to the temporal aspects and the cellular composition. (lu.se)
  • Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
Tissue6
  • I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
  • I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. (nottingham.ac.uk)
  • Tissue engineering and function reconstruction through host remodeling and autologous cell recruitment effectively was shown to overcome the aforementioned limitations and represent a fundamental shift from cell-based approaches ( 6-8 ). (spandidos-publications.com)
  • Extracellular matrix (ECM) is a dynamic tissue that contributes to organ integrity and function, and its regulation of cell phenotype is a major aspect of cell biology. (atsjournals.org)
  • When lung tissue is irradiated by x-ray cells are injured by the direct or indirect action of radiation. (biomedcentral.com)
  • International bi-monthly journal of cell signaling, tissue protection, and translational research. (conditionmed.org)
Lineages2
  • Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. (biomedcentral.com)
  • In this group of CGIs, repressive polycomb group (PcG) complexes act to block transcription in inappropriate lineages or at non-expressing stages during the differentiation programme. (biomedcentral.com)
Progenitor cell3
  • for hematopoietic stem and progenitor cell function and mast cell differentiation (22, 23). (researchhunt.com)
  • Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
  • A key challenge is to understand how state, but must also afford flexibility in cell-fate choice to permit the different cell-fate options confronting stem and progenitor cell-type diversification and differentiation in response to cells are selected and coordinated such that adoption of a given intrinsic cues or extrinsic signals. (lu.se)
Biology9
  • Nature Cell Biology. (wikipedia.org)
  • I cover mainly cell biology and physiology. (nottingham.ac.uk)
  • Understanding transitions from one cell type or state to another is in the focus of modern molecular biology. (nature.com)
  • 4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
  • The annual meeting of the Indian Society of Cell Biology, the "XXXI All India Cell Biology Conference & Symposium on Stem Cells: Application and Prospects" was held at Banaras Hindu University, Varanasi, from 14th to 16th December, 2007 with Dr R Raman as its Covenor. (ifcbiol.com)
  • Dr S K Apte, President, Indian Society of Cell Biology welcomed the guests on the behalf of the society and delivered the Presidential address. (ifcbiol.com)
  • The conference included the Prof J Das Memorial Lecture by Dr V Nagaraja, Indian Institute of Science, Bangalore, 14 invited talks, 21 proffered oral presentations by students and senior members, 59 poster presentations, an interactive session on research methodologies and the meeting of Executive Committee and General Body of Indian Society of Cell Biology. (ifcbiol.com)
  • His visit was fully supported by the International Federation of Cell Biology. (ifcbiol.com)
  • Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
Lineage cells1
Myogenic1
  • We had previously found that iSN04 promoted myogenic precursor cells (myoblasts) to differentiate into skeletal muscle cells and had hypothesized that the aptamer also enhanced differentiation of pluripotent stem cells. (shinshu-u.ac.jp)
Pathways3
  • In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • In terminally differentiated cell fate is coupled to appropriate regulation of the alternative cells, transcriptional networks must be stable and irreversible, pathways. (lu.se)
Retinoic Acid1
  • All-trans retinoic acid (RA) is the most active metabolite of vitamin A. Several studies have described a pivotal role for RA signalling in different biological processes such as cell growth and differentiation, embryonic development and organogenesis. (mdpi.com)
Bone marrow2
  • The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
  • Myelodysplastic syndrome (MDS) in childhood encompasses a diverse group of bone marrow disorders that share a common clonal defect of stem cells and that result in ineffective hematopoiesis with dysplastic changes in the marrow. (medscape.com)
Pancreatic1
  • The murine embryonic stem (ES) cell line, ES-D3 was used in this project as a model for (a) investigating controversial aspects of current protocols used for the derivation of (3 cell types from ES cells and (b) investigating the potential to regulate the differentiation of ES cells into endodermal derivatives 1 e 0-1 ike cells, using extra-cellular factors previously associated with various aspects of pancreatic development. (dcu.ie)
Vivo1
  • These results demonstrate that ectopic expression of Lhx2 confers an in vivo engrafting capacity to ESC/iPSC-derived hematopoietic cells and in vivo behavior of iPSC-derived hematopoietic cells is almost identical to that of ESC-derived cells. (ebiotrade.com)
Populations4
  • Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
  • However, given that bivalent domains can still be observed-albeit in lower proportion-in unipotent cells such as T cells and MEFs, an admixture of cell populations appears to be an unlikely explanation for the observed coexistence of these marks. (researchgate.net)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
Fate3
  • Fundamental questions in organogenesis are related to the identification of a simplified model which is able to mimic the molecular mechanisms involved in pattern organization and cell fate determination as well. (razavihospital.ir)
  • This raises a pertinent question: Can regulating the fate of stem cells in clinical settings and at scale be made more economical? (shinshu-u.ac.jp)
  • 2008). Historically, this concept is highlighted by the experi- factors are key intrinsic regulators of these fate decisions and mental phenomenon of lineage reprogramming, for example, that fate choice involves modulating networks of transcription by the conversion of fibroblasts to muscles cells following trans- factors. (lu.se)
Hematopoiesis1
  • Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)
Differentiate2
  • MSCs can not only differentiate into lung alveolar epithelial cells and secrete anti-inflammatory factors, but they also deliver some vehicles for gene therapy in repairing the injured lung, which provides new ideas for managing RILI. (biomedcentral.com)
  • However, there is also evidence to suggest that undifferentiated cells can both sense the mechanical properties of their environment and differentiate accordingly. (ox.ac.uk)
Cellular1
  • Thus, it has been argued that the observed bivalency simply reflects cellular heterogeneity arising from the averaging of cells that carry either, but not both, marks at a given locus (Fig. 2). (researchgate.net)
Tissues1
  • Cells Tissues Organs (2011) 193 (4): 239-252. (karger.com)
Induction1
  • The KSL cell induction frequency was superior to the case of Hoxb4. (ebiotrade.com)
Protein8
  • To systematically study such transitions, we introduce a method that simultaneously measures protein expression and thermal stability changes in cells and provide the web-based visualization tool ProteoTracker. (nature.com)
  • These transitions are enacted by profound changes in protein composition of the cells. (nature.com)
  • The combined proteomic approach called PISA-Express implemented here uses just two samples per replicate analysis of a cell type (Fig. 1a, b ) to assess both the protein abundance and solubility. (nature.com)
  • Fig. 1: Protein thermal stability and expression changes in cell-type transitions. (nature.com)
  • b Cells were collected and heated in a narrow temperature range, with one sample incubated at 37 °C for expression measurements, protein aggregates were eliminated by ultracentrifugation and the soluble proteins were digested. (nature.com)
  • The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. (biomedcentral.com)
  • To further promote differentiation and to increase mechanical signaling, Notch signaling was activated by presenting cells with fibronectin and DLL1 protein. (siu.edu)
  • The differentiation was found to have a pronounced effect in the presence of fibronectin and DLL1 protein together with the withdrawal of LIF. (siu.edu)
Microenvironment1
Antigen3
  • 2020 ) Chimeric antigen receptor T-cell therapy after allogeneic stem cell transplant for relapsed/refractory large B-cell lymphoma. (neurotree.org)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
  • The stage-specific embryonic antigen 4 (SSEA4) is brain. (lu.se)
Epithelial3
  • The process is hypothesized to involve epithelial cell dedifferentiation, interstitial cell transdifferentiation and/or activation of quiescent stem cells. (biomedcentral.com)
  • Adherens junctions are a ubiquitous type of intercellular junction and are present in both epithelial and non-epithelial cells [ 3 , 6 ], whereas desmosomes are adhesive junctions that confer tensile strength and resilience on cells and are present not only in epithelial cells but also in non-epithelial cells that endure mechanical stress, such as cardiac muscle and meninges [ 7 . (oncotarget.com)
  • FUT2 encodes an enzyme that produces histo-blood group antigens (HBGA) on the surface of epithelial cells and in mucosal secretions ( 21 , 22 ). (cdc.gov)
FACS1
  • fication and isolation via fluorescence-activated cell sort- ing (FACS). (lu.se)
Neurons2
  • Ectopic expression of Ngn2 was sufficient to form mature neurons from embryonic stem cells[5]. (bartleby.com)
  • We provide detailed protocols for the generation of lentiviral vectors, the transduction of hES-cells, and their differentia- tion into neurons. (lu.se)
Establishment1
Molecular1
Morphogenesis1
Extracellular matrix1
Affects2
  • This activates cascade of events which, in the end, affects oxidative status of the cells and provides robust protection against oxidative challenge. (springer.com)
  • Substrate stiffness affects early differentiation events in embryonic stem cells. (ox.ac.uk)
Tumors2
  • The commonest malignant tumors of bone are Meta-analysis of 14 randomized trials revealed a signifiplasma cell tumors (Chap. Side impact: dryness of mouth, pores and skin, blurred vision, loss of lodging constipation, bradycardia followed by tachycardia, difficulty with micturation, flushing [url=https://samoore.com/products/buy-finpecia-online/] hair loss jak inhibitor purchase finpecia 1 mg[/url]. (ehd.org)
  • Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)