Stem cardiomyocytes. Medical search. Frequent questions

They are a feasible and physiologically relevant alternative to embryonic stem cell, primary cells, and animal models. (creative-bioarray.com)
The most well-known type of pluripotent stem cell is the embryonic stem cell. (wikipedia.org)
Patient-matched embryonic stem cell lines can now be derived using somatic cell nuclear transfer (SCNT). (wikipedia.org)
They hypothesized that genes important to embryonic stem cell (ESC) function might be able to induce an embryonic state in adult cells. (wikipedia.org)
By performing promoter capture Hi-C (PCHi-C) on human embryonic stem cell-derived cardiomyocytes (hESC-CMs), we show that such promoter interactions are a key mechanism by which enhancers contact their target genes after hESC-CM differentiation from hESCs. (manchester.ac.uk)
In vitro toxicity data of these metabolites derived in the development of validated and accepted in vitro and in silico embryonic stem cell test were used as input in the PBK model to extrapolate in vitro concentration-response curves to predicted approaches is urgently needed. (cdc.gov)
This approach could therefore micromass test, and the embryonic stem cell test (EST) provide a means to reduce the need for animal testing in human risk assessment practices. (cdc.gov)
physiologically based animals because a mouse embryonic stem cell line is used. (cdc.gov)

Ranolazine rescues the heart failure phenotype of PLN-deficient human pluripotent stem cell-derived cardiomyocytes. (stembook.org)
Derived from workspace Reproducibility of the computational model of induced pluripotent stem-cell derived cardiomyocytes at changeset 430939c5c1b8 . (cellml.org)
In this study, we demonstrated an approach using human induced pluripotent stem cell‐derived atrial and ventricular cardiomyocytes (hiPSC‐aCMs and hiPSC‐vCMs, respectively) coupled with a sophisticated optical mapping system for drug screening of atrial‐selective compounds in vitro. (sfu.ca)
Several applications of pluripotent stem cell (PSC)-derlved cardlomyocytes require elimination of undifferentiated cells. (elsevierpure.com)
Cardiac in vitro models have become increasingly obtainable and affordable with the optimization of human pluripotent stem cell-derived cardiomyocyte (hPSC-CM) differentiation. (mssm.edu)
A Mount Sinai-led team has developed a reproducible and scalable method to advance maturation of human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs)-;cells that support heart muscle contraction, generated in the lab from human stem cell lines-;which researchers say will improve approaches for disease modeling, regenerative therapies, and drug testing. (azolifesciences.com)
2022) PPARdelta activation induces metabolic and contractile maturation of human pluripotent stem cell-derived cardiomyocytes. (azolifesciences.com)
In this group, human pluripotent stem cell derived (Organ-on-Chip) models are being developed, mostly cardiovascular models. (tradecast.eu)
Berend van Meer has won the Hugo van Poelgeest prize 2020 for his research on human pluripotent stem cell derived cardiomyocytes for disease modelling and drug discovery. (tradecast.eu)
In Chapter 3, I used human pluripotent stem cell (hPSC)-derived ventricular and atrial cells to study the toxicity of ibrutinib, a novel Bruton's tyrosine kinase (BTK) inhibitor, which has demonstrated benefit in B cell cancers, but is associated with atrial fibrillation. (sfu.ca)
Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from a somatic cell. (wikipedia.org)
citation needed] Since iPSCs can be derived directly from adult tissues, they not only bypass the need for embryos, but can be made in a patient-matched manner, which means that each individual could have their own pluripotent stem cell line. (wikipedia.org)
Induced pluripotent stem cell-derived cardiomyocytes beating in substrate. (medgadget.com)
CDI will also use the proceeds for the expansion of its product lines beyond the current iCell Cardiomyocytes product offering, and to expand its commercial organisation to meet the growing demand for induced pluripotent stem cell (iPSC) based products. (pharmaceutical-technology.com)
The transplantation of individual pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) is a promising technique to treat myocardial infarction and reverse heart failure but to time the contractile benefit generally in most studies remains humble. (healthcarecoremeasures.com)
Background Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are in vitro models with the clear advantages of their human origin and suitability for human disease investigations. (unibo.it)
Human-induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) have shed great light on cardiac regenerative medicine and specifically myocardial repair in heart failure patients. (biomedcentral.com)
Uninterrupted monitoring of drug effects in human-induced pluripotent stem cell-derived cardiomyocytes with bioluminescence Ca2+ microscopy. (ox.ac.uk)
Specialized cardiomyocytes like human induced pluripotent stem cell (iPSC)-derived cardiomyocytes are especially attractive cell models because they express GPCRs and ion channels that provide gene expression profiles as well as phenotypic characteristics while demonstrating spontaneous mechanical and electrical activity similar to native cardiac cells. (moleculardevices.com)
Human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) can be used to identify and evaluate the effects of potentially cardiotoxic compounds. (moleculardevices.com)
A study by Hashem et al using induced pluripotent stem cell-derived cardiomyocytes indicated that LAMP2 protein deficiency in Danon disease results in autophagic flux impairment, which in turn causes excessive oxidative stress and, subsequently, cardiomyocyte apoptosis. (medscape.com)

The isoform of contractile protein genes indicated that their muscle phenotype was similar to fetal ventricular cardiomyocytes. (elsevierpure.com)
I showed that ibrutinib has a dramatic impact on the cardiac electrophysiology of hPSC-derived atrial cardiomyocytes, without affecting hPSC-derived ventricular cardiomyocytes. (sfu.ca)

The diabetic formula used showed an increase in DNA damage and a decline in cell migration in mouse embryonic stem cells. (nottingham.ac.uk)
In this study, the interaction of mouse embryonic stem cells (mESC) with poly L-co-D, L lactic acid co-trimethylene carbonate (PLDLA-co-TMC) was assessed. (jscimedcentral.com)
the EST, which assesses the effects of compounds on the differentiation of mouse embryonic stem cells into contracting cardiomyocytes, can be used to rank the potency of chemicals within a series of alkoxyacetic acid metabolites formed from The implementation of the European REACH (Registration, glycol ethers. (cdc.gov)

The expression of cardiac markers in iPSC-derived cardiomyocytes and the electrical properties of the cells. (creative-bioarray.com)
Human iPSC-derived cardiomyocytes have been cocultured with sympathetic neurons, isolated from neonatal rat pups. (lscn.co.uk)
The iPSC technology was pioneered by Shinya Yamanaka and Kazutoshi Takahashi in Kyoto, Japan, who together showed in 2006 that the introduction of four specific genes (named Myc, Oct3/4, Sox2 and Klf4), collectively known as Yamanaka factors, encoding transcription factors could convert somatic cells into pluripotent stem cells. (wikipedia.org)
This product line is the first commercial product based on stem cells grown from adult tissue using iPSC technology to help pharmaceutical companies speed up the discovery of safer, more effective drugs. (pharmaceutical-technology.com)
Cell labeling, tracking, and molecular biology techniques indicated that the grafted cardiomyocytes survived in the rat heart 1 month after iPSC-CM transplantation. (biomedcentral.com)
iPSC-derived cardiomyocytes form a synchronously beating monolayer that can be used to reliably reproduce drug-associated, cardio-physiologic phenotypes using a fast, kinetic fluorescence assay that monitors changes in intracellular calcium oscillations. (moleculardevices.com)
The assays use iPSC-derived cardiomyocytes loaded with a calcium-sensitive dye and allow you to monitor the compound impact on oscillating calcium within the cells as they beat. (moleculardevices.com)
Spontaneous calcium oscillation acquired with FLIPR Calcium Assay Kit and the FLIPR system from iPSC cardiomyocytes or 3D cardiac spheroids can be paired with cytotoxicity and mitochondrial integrity data from the ImageXpress confocal system for comprehensive phenotypic analysis and bioactivity profiling of both pharmaceutical and environmental compounds. (moleculardevices.com)

iPSCs have great replicative ability and demonstrated potential to form functional cardiomyocytes (CMs). (creative-bioarray.com)
Creative Bioarray provides top quality service for directed-differentiation of iPSCs into cardiomyocytes using our proprietary induction protocol and reagents. (creative-bioarray.com)
1. Lineage-committed cardiomyocytes differentiated from iPSCs. (creative-bioarray.com)
Unlike the first generation of iPSCs, these second generation iPSCs produced viable chimeric mice and contributed to the mouse germline, thereby achieving the 'gold standard' for pluripotent stem cells. (wikipedia.org)
Researchers at Michigan Tech and Harvard Medical School have developed a new way to create cardiomyocytes from induced pluripotent stem cells (iPSCs), for improved cardiac cell therapy. (medgadget.com)
iPSCs show significant potential for cardiac regenerative medicine, as researchers can use biochemical cues to "program" them into a cardiomyocyte-like cell, which shows regenerative efficacy in damaged cardiac muscle. (medgadget.com)
iCell Cardiomyocytes are human heart cells derived from iPSCs. (pharmaceutical-technology.com)

The ability of the human induced pluripotent stem cells (hiPSCs) to differentiate to any cell type including cardiomyocytes, while carrying patients' complex genetic backgrounds, has made them a promising and powerful tool for drug screening, assessing the cardiotoxicity of chemotherapeutic agents, and studying inherited cardiac diseases in vitro by recapitulating their cellular phenotypes. (sfu.ca)
Since the discovery of human induced pluripotent stem cells (hiPSCs), numerous strategies have been established to efficiently derive cardiomyocytes from hiPSCs (hiPSC-CMs). (healthdatagateway.org)
Oxytocin - but none of 14 other neurohormones tested here - stimulates cultures of human Induced Pluripotent Stem Cells (hIPSCs) to become EpiPCs, at up to twice the basal rate: a much stronger effect than other molecules previously shown to stimulate EpiPC production in mice. (scitechdaily.com)
The FLIPR Penta system allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into cardiomyocytes and neurons, up to 100 times per second and quickly cherry pick events such as Early Afterdepolarization-like events (EAD-like events), fibrillation, and irregular beating. (moleculardevices.com)

Creative Bioarray is an experienced and outstanding provider of Cardiomyocyte Differentiation service as well as differentiation kit. (creative-bioarray.com)

Functional CMs derived from human pluripotent stem cells (hPSCs) would provide an unprecedented cell source for disease modeling, drug discovery, and cell transplantation therapy for CVD patients [ 3 ]. (biomedcentral.com)
We deliver ready-to-use, functional cardiomyocytes that are desirable in vitro models for high content toxicity and drug screening, and cell regeneration research. (creative-bioarray.com)
It has been thought that functional myocardial repair and regeneration could be regulated by stem cell-cardiomyocyte contact. (syr.edu)
Unfortunately stem cell therapeutics don't have high success rates partly because the cells are not mature and fully functional. (medgadget.com)
This results in more robust, mature and functional cardiomyocyte-like cells. (medgadget.com)
Myocardial infarction (MI), the most common heart disease, is characterized by a significant reduction in the number of functional cardiomyocytes and leads to the development of progressive heart failure. (biomedcentral.com)

The effect of reactive oxygen species on cardiomyocyte differentiation of pluripotent stem cells. (musc.edu)

By combining GmNL(Ca2+) with this adaptation, we could image spontaneous Ca2+ transients in cultured cardiomyocytes and phenotype their response to well-known drugs without accessing the sample directly. (ox.ac.uk)
We previously showed a link between the RyR2 -H29D mutation and a clinical phenotype of short-coupled PMVT at rest using patient -specific hiPSC -derived cardiomyocytes ( hiPSC -CMs). (bvsalud.org)

We have isolated a cardiomyogenic cell line (CMG cell) from murine bone marrow mesenchymal stem cells. (elsevierpure.com)
Fukuda, K 2002, ' Reprogramming of bone marrow mesenchymal stem cells into cardiomyocytes ', Comptes Rendus - Biologies , vol. 325, no. 10, pp. 1027-1038. (elsevierpure.com)
Adult human bone marrow (BM) is the major source of mesenchymal stem cells (MSC) for cell-based therapies. (journalcra.com)
This study focuses to identify, isolate and characterize the mesenchymal stem cells that are believed to be present in BM. (journalcra.com)
And hence, this study suggests that human bone marrow is a potential source of mesenchymal stem cells that could be differentiated in to ccardiomyocytes and used for cell based therapies for treating various cardiovascular diseases. (journalcra.com)
These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)

Using photolithography to shape a polydimethylsiloxane substrate and micropatterning to alter the substrate surface, the researchers developed 3D molds that mimic the natural forces and pressures that cardiomyocytes experience in the heart. (medgadget.com)

Non-thermal disruption of β-adrenergic receptor-activated Ca2+ signalling and apoptosis in human ES-derived cardiomyocytes by microwave electric fields at 2.4 GHz. (swansea.ac.uk)

Cells from the blood samples are reprogrammed into clinical-grade human induced pluripotent stem cells, which can be distributed to recipients for regenerative medicine. (wjgnet.com)
Pluripotent stem cells hold promise in the field of regenerative medicine. (wikipedia.org)

Stem Cell Res Ther;14(1): 266, 2023 09 23. (bvsalud.org)

Among the current biomaterial applications, the use of biopolymers as cell scaffolds could aid the development of stem cell-based therapies aimed at replacing tissue loss due to injuries or diseases, such as during myocardial infarction. (jscimedcentral.com)
The work of Berend aimed to understand how well these stem cell based cardiac models can predict the effect of (well-known) drug therapies in patients. (tradecast.eu)
Understanding how stem cells interact with cardiomyocytes is crucial for cell-based therapies to restore the cardiomyocyte loss that occurs during myocardial infarction and other cardiac diseases. (syr.edu)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)
This explains why researchers are now focusing attention on developing stem cell therapies using postnatal stem cells donated by the patients themselves or their close relatives. (bvsalud.org)

Cardiomyocytes have been stained for alpha-actinin (red) and a marker of sympathetic neurons, tyrosine hydroxylase (green). (lscn.co.uk)
Our main focus is neural lineage (e.g. neurons and astrocytes), but other cell types can also be recorded (e.g. cardiomyocytes, β cells). (lu.se)

We after that cocultured outrageous type cardiomyocytes with either cardiomyocytes or fibroblasts overexpressing R1R2 and noticed greater than a twofold upsurge in the level and price of contraction of outrageous type cardiomyocytes. (healthcarecoremeasures.com)

Thus, this study shows that MSCs were isolated from human bone-marrow and subsequently differentiated into cardiomyocytes with 100% success rate. (journalcra.com)

Now, scientists from Michigan State University have demonstrated that oxytocin has yet another, previously unknown, function in zebrafish and human cell cultures: it stimulates stem cells from the heart's outer layer (epicardium) to migrate into its middle layer (myocardium), where they develop into cardiomyocytes, the muscle cells that cause heart contractions. (scitechdaily.com)
The system can also monitor changes in intracellular Ca2+ flux (calcium oscillations) associated with cardiomyocyte contractions using the EarlyTox Cardiotoxicity Kit . (moleculardevices.com)

These findings indicated that cell transplantation therapy for the patients with heart failure might possibly be achieved using the regenerated cardiomyocytes from autologous bone marrow cells in the near future. (elsevierpure.com)

Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)

These new EpiPCs then migrate to the zebrafish myocardium to develop into cardiomyocytes, blood vessels, and other important heart cells, to replace those which had been lost. (scitechdaily.com)

Cardiomyocytes are the cells that make up the cardiac muscles and are responsible for the heart's contractile function. (moleculardevices.com)

mEScell growth and differentiation on PLDLA-co-TMC membrane, demonstrated the cyto compatibility of this polymer, and provided evidence of minimal to no toxicity on the cells, allowing adhesion and cell proliferation, as well as differentiation into cardiomyocytes. (jscimedcentral.com)
This is done partly by proliferation of cardiomyocytes, but also by EpiPCs. (scitechdaily.com)

A well-established method of cardiomyocyte differentiation from PSCs provides an ideal source of cardiomyocytes and makes regenerative therapy for myocardial muscle damage a promising strategy. (biomedcentral.com)

The biochips will help us gain knowledge of contact-mediated interactions between stem cells and cardiomyocytes, which are fundamental for formulating a strategy to achieve stem cell-based cardiac tissue regeneration. (syr.edu)

These include contractile activity (morphological scoring system), cell activity, total protein content, ROS production, DNA damage, transmembrane proteins expression (connexin43, integrin β1) and stem cell migration. (nottingham.ac.uk)
In ESD3, 6-gingerol severely affected differentiation into cardiomyocytes cell activity and protein content at both low and high concentrations. (nottingham.ac.uk)
We found that the human cardiomyocytes developed patterns of protein organization identical to the three-step series seen in the model organisms cited above. (syr.edu)
The survival of grafted cardiomyocytes was confirmed by observing the fluorescent cell tracer Vybrant™ CM-DiI or expression of the enhanced green fluorescent protein (eGFP) in transplanted cells, or survival was demonstrated by polymerase chain reaction (PCR)-based detection of human mitochondrial DNA. (biomedcentral.com)

ScreenWorks® Peak Pro Software offers the ability to analyze multi-peak calcium oscillation responses of cells-in particular, cardiomyocyte beat rate as well as temporal characteristics of peaks including rise, decrease, and amplitude. (moleculardevices.com)
Personalized medicine in the dish to prevent calcium leak associated with short-coupled polymorphic ventricular tachycardia in patient-derived cardiomyocytes. (bvsalud.org)

Outcomes hESC-CM contractility is certainly elevated with R1R2 overexpression Our prior work shows that R1R2 overexpression leads to elevated contractility in neonatal and mature adult rat cardiomyocytes[15 16 We initial confirmed that overexpression of R1R2 leads to similar boosts in contraction magnitude and speed in hESC-CMs. (healthcarecoremeasures.com)

The characterization of this material was made by means of SEM, DSC, TGA, GPC, Tensile Test, Viability Assay, and stem cells behavior by qPCR and Immunofluorescence techniques. (jscimedcentral.com)

Upon introduction of reprogramming factors, cells begin to form colonies that resemble pluripotent stem cells, which can be isolated based on their morphology, conditions that select for their growth, or through expression of surface markers or reporter genes. (wikipedia.org)
As the cells mature, they begin to beat, and more closely resemble mature, native cardiomyocytes. (medgadget.com)

chick embryonic heart micromass (MM) and mouse embryonic D3 stem cells (ESD3). (nottingham.ac.uk)

The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)
Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)

These lab-fabricated, fluorescently stained cardiomyocytes-heart muscle cells-exhibit the maturation and functionality of heart cells grown within a heart. (medgadget.com)
Programming induced pluripotent stem cells into heart muscle cells requires biochemical and biomechanical cues. (medgadget.com)
After a heart attack, cardiomyocytes often die off in large numbers. (scitechdaily.com)
Previous research has revealed, however, that a subset of cells in the epicardium may be reprogrammed to become stem-like cells known as Epicardium-derived Progenitor Cells (EpiPCs), which can regenerate not only cardiomyocytes but also other kinds of heart cells. (scitechdaily.com)
Heart regenerative therapy, such as transplantation of myocytes [ 4 ] and cardiomyocytes to replace lost cells, has become a new strategy for the treatment of heart failure caused by MI [ 5 ]. (biomedcentral.com)
At the Stem Cell Differentiation and Cytogenetics Group, we are interested in the effects of ionizing radiation on organs such as the brain, the heart, and the lung trying to elucidate the risks for patients treated with ionizing radiation as well as for astronauts, who are inevitably exposed to ionizing radiation on their missions into space. (gsi.de)
Particularly, human embryonic stem cells (hES cells), which can give rise to all tissues of an organism (Figure A), are used to generate mature cells of the heart (Figure B) allowing us to study radiation/microgravity-induced cardiovascular alterations, which may occur during long-term space missions. (gsi.de)

The results showed that the biochip design allows defined stem cell-cardiomyocyte contact-mode formation, which can be used to determine specific cellular interactions, including electrical coupling, mechanical coupling, and mitochondria transfer. (syr.edu)

A) Colonies of human embryonic stem cells, bright field microscopy, scale bar: 100µm. (gsi.de)

Further, when those MSCs were analyzed for their specific marker expression using flowcytometry, they were found to be positive for CD29, CD73, CD90, and CD105 and negative for CD34 and CD45 stem cell markers respectively. (journalcra.com)
Apart from this, it was observed that those MSCs, when induced to differentiate into cardiomyocytes, they successfully differentiated into cardiomyocyte like cells with flat, wide, myotube like structure with sarcomeric organization, which were later confirmed to be cardiomyocytes by qRT-PCR. (journalcra.com)

The research contributes to understanding the relevance of stem cell based models and strengthens the confidence regulators and pharmaceutical companies have in such models as animal alternatives in the drug development pipeline. (tradecast.eu)
We welcome candidates who can contribute to the diversity and excellence of our academic community.Description Postdoctoral Fellowship in Stem Cells The Cardiomyocyte Stem Cell Laboratory at Emory University School of Medicine is seeking a postdoctoral fellow to work on human induced pluripotent stem cells (http://www.pedsresearch.org/research-group/chunhui-xu-lab). (cytopathology.org)
Cellular Dynamics International (CDI) has raised $40.6m to increase the production capacity for its iCell Cardiomyocytes and to initiate additional human tissue cell products for biomedical and pharmaceutical drug development and safety research. (pharmaceutical-technology.com)
We initial performed intracellular dye transfer research using dATP conjugated to fluorescein and confirmed rapid difference junction-mediated transfer between cardiomyocytes. (healthcarecoremeasures.com)
The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)

And while animal models predicted less than 70% correctly, the human stem cell based models predicted almost 80% of the expected effects correctly. (tradecast.eu)

Cardiomyocytes (CMs) derived from human pluripotent stem cells (hPSCs) provide a valuable cell source for regenerative therapy, disease modeling, and drug screening. (biomedcentral.com)
Our aim is to determine if the same pathway is followed in human cardiomyocytes derived from human inducible pluripotent stem cells. (syr.edu)
OBJECTIVE: Cardiomyocytes derived from human-induced pluripotent stem cells are a powerful platform for high-throughput drug screening in vitro. (ox.ac.uk)

Anatomy44

Organisms13

Diseases18

Chemicals and Drugs83

Analytical, Diagnostic and Therapeutic Techniques and Equipment39

Phenomena and Processes61

Disciplines and Occupations3

Technology, Industry, Agriculture1

Information Science2

Embryonic stem8

Pluripotent stem21

Ventricular cardiomyocytes2

Mouse embryonic stem3

IPSC8

IPSCs7

HiPSCs4

Differentiation Kit1

Functional6

Reactive oxygen1

Phenotype2

Mesenchymal6

Substrate1

Apoptosis1

Regenerative medicine2

20231

Therapies5

Neurons2

Fibroblasts1

Subsequently1

Contractions2

Autologous1

Bone marrow1

Myocardium1

Heart's1

Proliferation2

Myocardial1

Cell based cardiac1

Protein4

Calcium2

Neonatal1

Behavior1

Resemble2

Chick1

Adult2

Heart7

Electrical1

Colonies1

MSCs2

Research5

Human stem cell1

Derived from human3