• Culture conditions of CD34(+) cells - either with or without mesenchymal stromal cells (MSCs) - had relatively little impact on DNAm, although proliferation is greatly increased by stromal support. (nih.gov)
  • Mesenchymal stem cell medium was used in the control groups. (scielo.br)
  • Adipose tissue (AT) represents a commonly used source of mesenchymal stem/stromal cells (MSCs) whose proregenerative potential has been widely investigated in multiple clinical trials worldwide. (mdpi.com)
  • We designed a study to find theoretical evidence for the induction, movement, fusion, proliferation, and safety of human adipose mesenchymal stem cells (hADSCs) in intraocular application. (molvis.org)
  • Therefore, in this study, we assessed the ability of human adipose mesenchymal stem cells (hADSCs) to express the characteristics of ECs after induction in vitro to evaluate the theoretical feasibility of maintaining or restoring the function of ECs after intraocular application. (molvis.org)
  • To compare the heterogeneities of human umbilical cord mesenchymal stem cells (HUCMSCs) derived from different donors and test their therapeutic variations in mouse liver fibrosis model. (researchsquare.com)
  • Mesenchymal stem cells (MSCs) have been widely tested for treating a variety of refectory medical indications such as type 1 diabetes mellitus, systemic lupus erythematosus, rheumatoid arthritis and Crohn's disease due to their multiple differentiation potentials and immunomodulation capability [1-5]. (researchsquare.com)
  • Mesenchymal stem cells (MSCs) are multipotent cells usually isolated from bone marrow, endometrium, adipose tissues, skin, and dental pulp. (techscience.com)
  • Mesenchymal stem cells (MSCs) are multipotent stromal cells capable of self-renewal and exhibit multilineage differentiation properties. (techscience.com)
  • The Mesenchymal and Tissue Stem Cell Committee, which belongs to the International Society for Cellular Therapy, stated that plastic-adherent properties are the minimum criteria for determining MSCs. (techscience.com)
  • While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
  • There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
  • Data suggests that CXCL12β mediates cells' survival by enhancing autophagy in bone marrow mesenchymal stem cells. (biolegend.com)
  • mesenchymal stem cells - placenta) differentiate into multipotency as precursors to formation of the cellular components. (hoajonline.com)
  • These CD4+ CD25+ T regulatory cells can be isolated from mesenchymal stem cells and CD34+ hematopoietic stem cells. (hoajonline.com)
  • This study aimed to evaluate the effect of mesenchymal stem cell (MSC)-derived exosomes on an immune-induced liver injury model. (biomedcentral.com)
  • 6,7 Mesenchymal stem cells (MSCs) have also been isolated from other sources, such as bone marrow, 8 the placenta, 9 muscle, 10 or blood. (jcadonline.com)
  • We are grateful to Gibco and Life Technologies AS for supplying CTS™ Dynabeads™ CD3/CD28 and Drs. Mengyu Wang and Hanne B. Scholz (Oslo University Hospital) for providing the mesenchymal stem cells. (opinvisindi.is)
  • Mesenchymal stem cells (MSCs) are ASCs, and were first described in 1966 by Friedenstein et al. (bvsalud.org)
  • 2 ] in 1994 and is defined by the expression of a variety of cell surface antigens, including the stem cell marker CD34, the pan-leukocyte marker CD45, and monocyte markers CD14 and CD11. (ersjournals.com)
  • A feature of fibrocytes is that they tend to lose the expression of haematopoietic markers, such as CD45 and CD34, within a short time frame once incorporated into the disease tissue. (ersjournals.com)
  • A significant proportion (two thirds) of CD45+ cells in the normal corneal stroma expressed CD34+ while no CD45- cells (ie keratocytes) co-expressed CD34. (arvojournals.org)
  • Immunophenotyping of hWMSCs by flow cytometry To confirm the derived MSCs, specific cell-surface antigens including CD45, CD44, CD34 and CD105 (Sigma, Germany) were probed using monoclonal antibodies and compared with cells treated with control isotype antibodies. (bioskinrevive.com)
  • Hematopoietic stem and progenitor cells (HPCs) can be maintained in vitro, but the vast majority of their progeny loses stemness during culture. (nih.gov)
  • The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early haematopoietic and vascular-associated progenitor cells. (wikipedia.org)
  • Collectively, these are referred to as hematopoietic stem and progenitor cells (HSPCs). (stemcell.com)
  • CD34 is the most commonly used cell surface marker to identify human HSPCs as it is expressed on HSCs, in addition to both multipotent and more differentiated progenitor cells of individual blood cell lineages. (stemcell.com)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • Hematopoietic stem and progenitor cells (HSPCs) isolated from human blood are frequently used as a normal comparator in AML studies. (ox.ac.uk)
  • The ability to genetically manipulate hematopoietic stem and progenitor cells (HSPCs) in particular has significantly advanced our understanding of the mechanisms that regulate hematopoiesis and is contributing to the development of novel cellular therapies. (stemcell.com)
  • Mouse monoclonal to CD34.D34 reacts with CD34 molecule, a 105-120 kDa heavily O-glycosylated transmembrane glycoprotein expressed on hematopoietic progenitor cells, vascular endothelium and some tissue fibroblasts. (imacst.com)
  • Effective expansion of umbilical cord blood hematopoietic stem/progenitor cells by regulation of microencapsulated osteoblasts under hypoxic condition. (ox.ac.uk)
  • The expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood (UCB) with the support of microencapsulated osteoblasts under hypoxia environment was investigated. (ox.ac.uk)
  • CD34 was first described on hematopoietic stem cells independently by Civin et al. (wikipedia.org)
  • It may also mediate the attachment of hematopoietic stem cells to bone marrow extracellular matrix or directly to stromal cells. (wikipedia.org)
  • Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. (wikipedia.org)
  • CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. (wikipedia.org)
  • The presence of CD34 on non-hematopoietic cells in various tissues has been linked to progenitor and adult stem cell phenotypes. (wikipedia.org)
  • CD34 is expressed in roughly 20% of murine haematopoietic stem cells, and can be stimulated and reversed. (wikipedia.org)
  • CD34+ is often used clinically to quantify the number of haemopoietic stem cells for use in haemopoietic stem cell transplantation. (wikipedia.org)
  • Antibodies are used to quantify and purify hematopoietic progenitor stem cells for research and for clinical bone marrow transplantation. (wikipedia.org)
  • i.e., they are multipotent hematopoietic stem cells. (wikipedia.org)
  • Injection of CD34+ hematopoietic stem cells has been clinically applied to treat various diseases including spinal cord injury, liver cirrhosis and peripheral vascular disease. (wikipedia.org)
  • This study was designed to determine the in vivo performance of three different materials as scaffolds for dental pulp stem cells (DPSC) undergoing induced odontogenic differentiation. (scielo.br)
  • Although different stem cells, morphogens and scaffolds can be combined to simulate natural odontogenic regeneration, it is crucial to determine which options most closely replicate the characteristics of human dental tissues. (scielo.br)
  • Dental pulp stem cells (DPSC) seem to be the most appropriate cell group for the regenerative approach. (scielo.br)
  • Dental pulp stem cells: state of the art and suggestions for a true translation of research into therapy. (scielo.br)
  • Odontogenic capability: bone marrow stromal stem cells versus dental pulp stem cells. (scielo.br)
  • The odontogenic differentiation of human dental pulp stem cells on nanofibrous poly(L-lactic acid) scaffolds in vitro and in vivo. (scielo.br)
  • Sonoyama W, Yamaza T, Gronthos S, Shi S. Multipotent stem cells in dental pulp. (scielo.br)
  • editors Culture of human stem cells. (scielo.br)
  • The CD117 antigen is expressed on very few normal bone marrow cells. (beckman.com)
  • The majority of CD117+ marrow cells co-express CD34. (beckman.com)
  • Hematopoietic stem cell transplantation (HSCT) involves the intravenous infusion of hematopoietic stem cells in order to reestablish blood cell production in patients whose bone marrow or immune system is damaged or defective. (medscape.com)
  • The National Marrow Donor Program (NMDP), founded in 1986, and the World Marrow Donor Association (WMDA), founded in 1988, were established to (1) locate and secure appropriate unrelated-donor HSCT sources for patients by promoting volunteer donation of bone marrow and peripheral blood stem cells in the community and (2) promote ethical practices of sharing stem cell sources by need, rather than by geographic location of the donor. (medscape.com)
  • The purpose is to deliver chemotherapy, immunotherapy, and/or radiation to eliminate malignancy, prevent rejection of new stem cells, and create space for the new cells. (medscape.com)
  • In addition, they act as antigen presenting cells and promote angiogenesis [ 8 ]. (ersjournals.com)
  • In contrast, the remaining population of CD45+ CD34- cells in the corneal stroma expressed CD11b, MHC Class II+ but not CD11c, and were found mostly in anterior and peripheral part of stroma. (arvojournals.org)
  • The larger population are CD34+ and resemble haemopoietic stem cells while the smaller population are CD34-CD11b+ MHC Class II+ macrophages. (arvojournals.org)
  • CD135 is a 130-160 kD type III tyrosine kinase receptor expressed on CD34 + hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. (biolegend.com)
  • Expressed on CD34 + hematopoietic stem cells, myelomonocytic progenitors, primitive B cell progenitors, and thymocytes. (biolegend.com)
  • Embryonic stem cells isolation primarily requires the consent of donors and can include the killing of fertilized eggs. (techscience.com)
  • The present invention provides stem cells enriched with healthy functional mitochondria, and therapeutic methods utilizing such cells for the alleviation of debilitating conditions, including aging, and age-related diseases as well as the debilitating effects of anti-cancer therapies in subjects in need thereof. (justia.com)
  • The present invention relates to stem cells enriched with functional mitochondria, and therapeutic methods utilizing such cells to diminish the debilitating effects of various conditions, including aging and age-related diseases as well as the debilitating effects of anti-cancer therapy treatments. (justia.com)
  • WO 2001/046401 discloses embryonic or stem-like cells produced by cross species nuclear transplantation. (justia.com)
  • WO 2013/002880 describes compositions and methods comprising bio-energetic agents for restoring the quality of aged oocytes, enhancing oogonial stem cells or improving derivatives thereof (e.g., cytoplasm or isolated mitochondria) for use in fertility-enhancing procedures. (justia.com)
  • Aberrantly expressed cytokines in the bone marrow (BM) niche are increasingly recognized as critical mediators of survival and expansion of leukemic stem cells. (haematologica.org)
  • To identify regulators of primitive chronic myeloid leukemia (CML) cells, we performed a high-content cytokine screen using primary CD34 + CD38 low chronic phase CML cells. (haematologica.org)
  • A pro-inflammatory environment is thought to provide a selective advantage for the leukemic stem cells (LSC). (haematologica.org)
  • The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. (origene.com)
  • [ 1 ] Antigen-presenting cells or histiocyte-like cells containing Birbeck granules have been identified within inflamed synovial tissues. (medscape.com)
  • [ 3 , 4 ] Langerhans cells are stellate dendritic cells that arise from CD34-positive bone marrow stem cells [ 5 ] and are not a member of the mononuclear phagocytic system. (medscape.com)
  • Counting your baby's cord blood stem cells: What do TNC, CD34+, CFU and other numbers mean? (cryo-cell.com)
  • The goal of cord blood banking is to capture as many stem cells as possible, but how do you know if your baby's cord blood collection is a good one? (cryo-cell.com)
  • There are currently several methods you can use to assess the potential future effectiveness of your child's cord blood collection including the collection's volume (in milliliters), the number of total cells (TNC count), the number of stem cells (CD34+ count), and a measurement of the stem cell functionality and potency (such as a colony-forming unit [CFU] count). (cryo-cell.com)
  • So more cord blood often equates to more cells, which often correlates to more stem cells, which often equates to better potency. (cryo-cell.com)
  • Increases in the volume of a cord blood correlate with increases in stem cells post-processing. (cryo-cell.com)
  • It is, however, not a rule, so a smaller collection could yield more stem cells than a slightly larger collection. (cryo-cell.com)
  • This is why it is important to more directly assess the number of stem cells in a collection to ensure its effectiveness in transplantation. (cryo-cell.com)
  • Nucleated cells include stem cells but also other immune system cells, and as such, stem cells can account for around 1% of the total nucleated cells. (cryo-cell.com)
  • The TNC count, therefore, gives us the total number of many cell types but not the number of stem cells exclusively. (cryo-cell.com)
  • To better determine the number of hematopoietic stem cells in a cord blood collection, scientists can also look for a specific protein on the surface of the cell. (cryo-cell.com)
  • This marker protein allows us to count stem cells more directly, but the marker is not exclusive to stem cells, so some other cell types will also be counted. (cryo-cell.com)
  • To count the number of cells that are positive for the CD34 protein (CD34+), a sample of the cord blood is mixed with a fluorescent dye that adheres to the CD34 antigen. (cryo-cell.com)
  • A flow cytometer uses a laser to pick up on the dye and count the number of CD34-positive cells. (cryo-cell.com)
  • PGCB says the median private cord blood collection of 60 mL equates to 1.8 million cells with the CD34 antigen. (cryo-cell.com)
  • While the number of stems cells in any cord blood collection is important, the ability for those stem cells to function and multiply after infusion is even more important. (cryo-cell.com)
  • CD133 + stem cells represent a promising subpopulation for innovative cell-based therapies in cardiovascular regeneration. (biomedcentral.com)
  • CD133 + stem cells were purified from patient-derived sternal bone marrow using the recently developed automatic CliniMACS Prodigy® BM-133 System (Prodigy). (biomedcentral.com)
  • In addition, the regenerative potential of purified stem cells was assessed 3 weeks after transplantation in immunodeficient mice which had been subjected to experimental myocardial infarction. (biomedcentral.com)
  • Adult stem cells remain of particular interest as therapeutic agents for cardiac regeneration in ischemic heart disease [ 1 - 4 ]. (biomedcentral.com)
  • Recently, several clinical trials showed an increased left ventricular ejection fraction (LVEF) and an improved regional perfusion following intramyocardial application of cluster of differentiation (CD) 133 + stem cells [ 5 - 9 ]. (biomedcentral.com)
  • According to the European Medicines Agency (EMA) BM-derived CD133 + stem cells intended for the regeneration of human ischemic heart tissue are classified as an advanced therapy medicinal product (ATMP) [ 17 ]. (biomedcentral.com)
  • Yet, CD133 + stem cells for clinical use are purified using manual or semi-automatic devices based on the conventional magnetic cell sorting (MACS®) technique. (biomedcentral.com)
  • In recent years, clinical trials with stem cells have taken the emerging field in many new directions. (biomedcentral.com)
  • More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)
  • These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
  • Placenta-derived stem cells are being considered for similar uses and are in Phase III clinical trial for critical limb ischemia by Israel's Pluristem Therapeutics. (biomedcentral.com)
  • A significant proportion of clinical studies that are underway involve bone marrow and cord blood stem cells for blood and immune disorders [ 3 ] and cancers. (biomedcentral.com)
  • The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
  • This Technical Bulletin provides instructions for CRISPR-Cas9 genome editing and maintenance of human CD34 + cells, including optimized pre- and post-editing culture conditions and methods to evaluate genome editing efficiency. (stemcell.com)
  • While CRISPR-Cas9 technology has been successfully applied in numerous cell lines, its application in primary human CD34 + cells has been hampered by challenges in efficient expression and delivery of CRISPR-Cas9 components. (stemcell.com)
  • The ArciTect™ CRISPR-Cas9 RNP is then prepared and delivered into CD34 + cells using electroporation, and cells are plated in StemSpan™ SFEM II supplemented with StemSpan™ CD34 + Expansion Supplement post-electroporation. (stemcell.com)
  • Additionally, edited CD34 + cells can be further cultured in conditions stimulating lineage-specific differentiation by combining StemSpan™ media with expansion supplements designed for the expansion and differentiation of progenitors to erythroid, myeloid, megakaryocyte, natural killer (NK), or T cell lineages. (stemcell.com)
  • Genetically distinct leukemic stem cells in human CD34- acute myeloid leukemia are arrested at a hemopoietic precursor-like stage. (ox.ac.uk)
  • 98% of cells are CD34(-), there are multiple, nonhierarchically arranged CD34(+) and CD34(-) LSC populations. (ox.ac.uk)
  • Also, CXCL12 is highly expressed in bone marrow stromal stem cells and regulates growth and survival. (biolegend.com)
  • In contrast, Y‐1K cells expressed CD34 stem cell antigen and CD41 megakaryocyte‐specific antigen. (elsevierpure.com)
  • This reduced differentiation of CD34+ hematopoietic progenitor stem cells could occur due to both infectious and non-communicable diseases and also due to their drug induced treatments. (hoajonline.com)
  • Therapeutic regeneration of molecularly engineered and transplanted autologous stem cells in neurological and cardiovascular diseases due to inability of self-regeneration or repair of endogenous stem cells at sites of inflammation or disease at various stages of progress for regeneration of the damaged tissue depending upon the sites and nature of degeneration. (hoajonline.com)
  • Tumors contain a minority population of cancer stem cells that maintain the tumor. (hoajonline.com)
  • In marked contrast to the cancer stem cells, the tumor cells without "stemness" have either no capacity or a markedly diminished capacity, to form new tumors. (hoajonline.com)
  • The tumor will rapidly recur if the therapy eliminates cancer cells but spares a significant population of the cancer stem cells. (hoajonline.com)
  • Cancer stem cellsand their susceptibility over their expected to be "downstream or progeny" cancer cells represent a target for therapeutic interventions to prevent latency and consequently relapse or metastasis of the malignant disease. (hoajonline.com)
  • Embryonic stem cells which are characteristic of totipotency differentiate into generations of the multiple organs. (hoajonline.com)
  • The pluripotent stem cells (eg. (hoajonline.com)
  • Retention of cellular phenotype is the hallmark of differentiation of pluripotent stem cells. (hoajonline.com)
  • Gene delivery through stem cells using specific viral vectors are also utilised to confer lineage specificity of transducedautologous or allogeneic stem cell gene expressionin the recipient while addressing graft versus host disease. (hoajonline.com)
  • The best hope for regeneration of these permanently damaged cells is optic stem cell therapy combined with continued drug treatments or other exogenous biomaterial implants depending upon the relevant ophthalmological condition. (hoajonline.com)
  • The expansion of HSPCs was evaluated through the total number of UCB mononuclear cells (MNCs) produced, their repopulating potential with the colony-forming unit assay (CFU-Cs) and CD34(+) phenotypic analysis with flow cytometry. (ox.ac.uk)
  • At the end of 7 days of culture, the UCB-MNCs, CFU-Cs and CD34(+) cells had achieved 18.7 +/- 1.6, 11.6 +/- 0.9 and 23.4 +/- 2-fold expansions, respectively, in the test groups. (ox.ac.uk)
  • Here we show that chimeric antigen receptor (CAR) T cells could successfully target an isoform of alkaline phosphatase, ALPL-1, which is highly and specifically expressed in primary and metastatic OS. (opinvisindi.is)
  • T cells transduced with these CAR constructs mediate efficient and effective cytotoxicity against ALPL-positive cells in in vitro settings and in state-of-the-art in vivo orthotopic models of primary and metastatic OS, without unexpected toxicities against hematopoietic stem cells or healthy tissues. (opinvisindi.is)
  • Objective: the aim of this study was to conduct a literature review of the types of stem cells of dental origin and their applications in Dentistry. (bvsalud.org)
  • Results: there are five main sources of stem cells of dental origin: stem cells from dental pulp of permanent teeth and deciduous teeth, apical papilla, periodontal ligament and dental follicle. (bvsalud.org)
  • Conclusion: stem cells from dental origin are an interesting alternative for research and application in regenerative therapies in Dentistry. (bvsalud.org)
  • Stem cells (SCs) are undifferentiated cells with self-renewal ability and capacity to differentiate into specialized cell types. (bvsalud.org)
  • 1,2 Regarding the origin, they can be classified as embryonic stem cells (ESCs) and adult stem cells (ASCs). (bvsalud.org)
  • 3 Embrionic stem cells (ESCs) are derived from the inner cell mass of the blastocyst and form all cell types, derived from the three germ layers, and are therefore pluripotent. (bvsalud.org)
  • Due to the ability to self-renew and to differentiate into cells that are found throughout the body, there is a great interest in using stem cells for the regeneration of injured tissues as well as to develop tissue-engineered implants and bio-hybrid organs, in order to restore tissue function. (bvsalud.org)
  • 8,9 Because dental stem cells (DSCs) are easy to obtain and present a great potential of differentiation, there has been a growing interest in their use in regenerative medicine for treatment of various human diseases. (bvsalud.org)
  • In human postnatal dental tissues, five main sources of DSCs have been identified: dental pulp stem cells (DPSCs), 11 stem cells from human exfoliated deciduous teeth (SHEDs), 12 periodontal ligament stem cells (PDLSCs), 13 dental follicle stem cells (DFSCs) 14 and stem cells from apical papilla (SCAPs). (bvsalud.org)
  • The patient received bone marrow cells (4.2 × 10 6 CD34-positive cells/kg) from an 8/10 human leukocyte antigen-matched, CMV-positive, unrelated donor. (cdc.gov)
  • Association of stromal cell-derived factor-1-3'A polymorphism to higher mobilization of hematopoietic stem cells CD34+ in Tunisian population. (cdc.gov)
  • ABSTRACT This study examined haematopoietic stem cells of 19 high-risk cases of myelodysplastic syndrome (MDS) for apoptotic and anti-apoptotic signals and cellular proliferation and correlated these with clinical and cytogenetic subtypes, particularly trisomy 8. (who.int)
  • The essence of MDS is damage of In the current work we examined All our patients were of the high-risk colony-forming units [4], but the defect haematopoietic stem cells of high-risk group and none of them was eligible of the haematopoietic stem cells is not MDS cases for apoptotic and anti-apop- for stem cell transplantation. (who.int)
  • 2005). Notch1 and syndecan-1 potent human embryonic stem (ES) cells. (lu.se)
  • The HUCMSCs derived different donors have individual heterogeneity, which potentially lead to distinct therapeutic outcomes in mouse liver fibrosis, indicating we could make use of the donor-variation of MSCs to screen out guaranteed general indicators of MSCs for specific diseases in further stem cell therapy. (researchsquare.com)
  • CD34(-) LSCs have disordered global transcription profiles, but these profiles are enriched for transcriptional signatures of normal CD34(-) mature granulocyte-macrophage precursors, downstream of progenitors. (ox.ac.uk)
  • Although anti-CD19 chimeric antigen receptor (CAR) T-cell therapy shows good efficacy in patients with relapsed/refractory B-cell acute lymphoblastic leukemia (r/r B-ALL), it fails to improve long-term leukemia-free survival (LFS). (biomedcentral.com)
  • CD34 derives its name from the cluster of differentiation protocol that identifies cell surface antigens. (wikipedia.org)
  • Cell markers, or surface antigens, are molecules located on a cell's membrane used to identify specific cell types, their lineage, and their stage in the differentiation process according to the presence or absence of the expression of defined markers. (stemcell.com)
  • Later studies have reported that low rhodamine retention identifies LT-HSCs within the Lin−CD34+CD38− population. (wikipedia.org)
  • Inhibition of chemokine receptor 4, the receptor for the stem cell mobiliser stromal-derived factor-1, significantly prevented and reversed hypoxia-induced pulmonary hypertension in neonatal mice [ 13 ]. (ersjournals.com)
  • CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. (thermofisher.com)
  • The stage-specific embryonic antigen 4 (SSEA4) is com- isolate the NSCs from neonatal mice and rats (Campos monly used as a cell surface marker to identify the pluri- et al. (lu.se)
  • The stage-specific embryonic antigen 4 (SSEA4) is brain. (lu.se)
  • The 581 monoclonal antibody specifically binds to CD34, a sialomucin-like type I transmembrane glycoprotein. (bdbiosciences.com)
  • The 581 antibody blocks reactivity of another anti-CD34 monoclonal antibody, 8G12. (bdbiosciences.com)
  • CD34 is a transmembrane phosphoglycoprotein protein encoded by the CD34 gene in humans, mice, rats and other species. (wikipedia.org)
  • CD34 is a transmembrane phosphoglycoprotein that was first identified on HSPCs, but its expression is also present on other cell types, such as vascular tissue. (stemcell.com)
  • This document also includes a case study detailing optimization of pre- and post-editing culture conditions to support a high efficiency genome editing workflow in CD34 + HSPCs. (stemcell.com)
  • Early attempts to apply CRISPR-Cas9 for genome editing in CD34 + HSPCs used plasmids for Cas9 and guide RNA (gRNA) expression, which resulted in low targeting efficiency and high toxicity. (stemcell.com)
  • CD34 may also play a role as an adhesion molecule since it binds to CD62E and CD62L. (bdbiosciences.com)
  • Human recombinant protein fragment corresponding to amino acids 312-385 of human CD34(NP_001020280) produced in E.coli. (origene.com)
  • Recombinant fusion protein containing a sequence corresponding to amino acids 32-290 of human CD34 (NP_001020280.1). (prosci-inc.com)
  • Maturation of the terminally differentiated cell loses the phenotype of the self-renewal antigen (eg. (hoajonline.com)
  • Malignant transformation usually occurs at the pluripotent stem cell level, although it sometimes involves a committed stem cell with more limited capacity for self-renewal. (msdmanuals.com)
  • Human HSCs express the CD34 marker. (wikipedia.org)
  • In human acute myeloid leukemia (AML), current models suggest transformation creates leukemic stem cell (LSC) populations arrested at a progenitor-like stage expressing cell surface CD34. (ox.ac.uk)
  • CD133+), but are rarely codetected with the neural stem dents, very few human-specific NSC markers have been cell (NSC) marker CD15. (lu.se)
  • Our understanding of the perturbation of normal cellular differentiation hierarchies to create tumor-propagating stem cell populations is incomplete. (ox.ac.uk)
  • Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. (wikipedia.org)
  • Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. (wikipedia.org)
  • Regardless of its mode of action, under all circumstances CD34, and its relatives podocalyxin and endoglycan, facilitates cell migration. (wikipedia.org)
  • Stem cell infusion is a relatively simple process that is performed at the bedside. (medscape.com)
  • Our hospital is approved to be qualified for implementing stem cell clinic trials by government agencies [19]. (researchsquare.com)
  • Different approaches to evaluate the future of biomaterials and stem cell properties have been developed. (techscience.com)
  • Dr. Abutalib is Assistant Director in the Stem Cell Transplant & Cell Therapy Program at Cancer Treatment Centers of America in Chicago. (ascopost.com)
  • Chronic myeloid leukemia (CML) is a myeloproliferative neoplasm caused by an acquired 9;22-chromosomal translocation in a hematopoietic stem cell (HSC) resulting in the expression of the BCR-ABL1 fusion protein. (haematologica.org)
  • The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
  • But unlike mature precursors, LSCs express multiple normal stem cell transcriptional regulators previously implicated in LSC function. (ox.ac.uk)
  • CXCL12/CXCR4 pair plays a key role in maintaining proliferation and survival of bone marrow stem cell subpopulations under stress conditions. (biolegend.com)
  • Therefore, to treat cancer effectively, the cancer stem cell must be eliminated. (hoajonline.com)
  • Some studies report a higher frequency of KIPyV DNA detection in hematopoietic stem cell transplant (HSCT) recipients ( 5 - 7 ) than in immunocompetent patients. (cdc.gov)
  • The theoretical basis for marrow failure includes primary defects in or damage to the stem cell or the marrow microenvironment. (medscape.com)
  • External insults (eg, infections, radiation, drugs) may disrupt stem cell homeostasis in marrow environment, leading to altered growth. (medscape.com)
  • VC 2006 Wiley-Liss, Inc. a glycolipid antigen with globo-series carbohydrate core structures (Kannagi et al. (lu.se)