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  • particulate
  • 000137589 001__ 137589 000137589 005__ 20181203021605.0 000137589 0247_ $$2doi$$a10.1021/ac0109467 000137589 022__ $$a00032700 000137589 037__ $$aARTICLE 000137589 245__ $$aAn Integrated Fritless Colum for On-Chip Capillary Electrochromatography with Conventional Stationary Phases 000137589 260__ $$c2002 000137589 269__ $$a2002 000137589 336__ $$aJournal Articles 000137589 500__ $$a280 000137589 520__ $$aA new polymer device for use with conventional particulate stationary phases for on-chip, fritless, capillary electrochromatography (CEC) has been realized. (epfl.ch)
  • mobile phase
  • of the components for a stationary phase (a solid or liquid) as a mobile phase (a liquid or gas) passes over the stationary phase. (britannica.com)
  • The mobile phase flows through the packed bed or column. (britannica.com)
  • mobile phase, and a contiguous stationary phase. (britannica.com)
  • The mobile phase may be either a liquid or a gas, while the stationary phase is either a solid or a liquid. (britannica.com)
  • Efforts to lower mobile phase organic content and raise pH to improve retention were ineffective using the alkyl phase. (sigmaaldrich.com)
  • In this case, 2-AMP is well retained and separated from the parent molecule using a simple mobile phase. (sigmaaldrich.com)
  • Figure 1 shows an excellent separation of serine enantiomers using a very simple, LC-MS compatible mobile phase. (sigmaaldrich.com)
  • Although the MeOH content in the mobile phase did not significantly influence the resolution, MeOH enhanced the peak intensities of most of the steroids with an atmospheric pressure chemical ionization (APCI) interface. (go.jp)
  • Use of other salts such as 100-300mM sodium perchlorate, which are soluble in high-organic solvent mixtures (ca. 70%-90% acetonitrile), can be used to increase the mobile phase polarity to effect elution. (wikipedia.org)
  • The sampler brings the sample mixture into the mobile phase stream which carries it into the column. (wikipedia.org)
  • The pumps deliver the desired flow and composition of the mobile phase through the column. (wikipedia.org)
  • The sample mixture to be separated and analyzed is introduced, in a discrete small volume (typically microliters), into the stream of mobile phase percolating through the column. (wikipedia.org)
  • The velocity of each component depends on its chemical nature, on the nature of the stationary phase (column) and on the composition of the mobile phase. (wikipedia.org)
  • The separation took place under isocratic conditions using pure water as the mobile phase, and controlled the temperature using a water bath. (wikipedia.org)
  • Avoiding the use of pressure to introduce the mobile phase into the column, results in a number of important advantages. (wikipedia.org)
  • A second advantage of using electroosmosis to pass the mobile phase into the column is the plug-like flow velocity profile of EOF, which reduces the solute dispersion in the column, increasing column efficiency. (wikipedia.org)
  • These will then less rapidly eluted by the mobile phase consisted of acetonitrile, heteroduplexes compared to the first pass so that the front detector UV (Ultraviolet). (wikipedia.org)
  • For the dry method, the column is first filled with dry stationary phase powder, followed by the addition of mobile phase, which is flushed through the column until it is completely wet, and from this point is never allowed to run dry. (wikipedia.org)
  • However, the maximum flow rate is limited because a finite time is required for the analyte to equilibrate between the stationary phase and mobile phase, see Van Deemter's equation. (wikipedia.org)
  • The mixture is dissolved in a fluid called the mobile phase, which carries it through a structure holding another material called the stationary phase. (wikipedia.org)
  • The eluate is the mobile phase leaving the column. (wikipedia.org)
  • The mobile phase is the phase that moves in a definite direction. (wikipedia.org)
  • The mobile phase consists of the sample being separated/analyzed and the solvent that moves the sample through the column. (wikipedia.org)
  • Synthesis
  • At least three known genes including xthA and katE are regulated by a sigma factor, KatF(RpoS), whose synthesis is turned on during the stationary phase. (wikipedia.org)
  • ClpAP seems to play an indirect role in maintaining ongoing dps synthesis during stationary phase Cavities formed by Dps and ferritin proteins have been successfully used as the reaction chamber for the fabrication of metal nanoparticles (NPs). (wikipedia.org)
  • elution
  • There are also instances of the elution times increasing below 15 °C, which most likely can be attributed to the lower temperatures' effects on mass transfer playing a more significant role on retention than the stationary phase behavior. (wikipedia.org)
  • cultures
  • We report the isolation and characterization of quiescent and nonquiescent cells from stationary-phase (SP) yeast cultures by density-gradient centrifugation. (rupress.org)
  • The group discovered regulatory systems exclusive to cells in this non-growing state and found that mutants with greater fitness in stationary phase evolved and rapidly took over the cultures. (wikipedia.org)
  • Zambrano MM, Siegele DA, Almirón M, Tormo A, Kolter R. Microbial competition: Escherichia coli mutants that take over stationary phase cultures. (wikipedia.org)
  • PNIPAAm
  • Where pure PNIPAAm was unable to resolve hydrophobic steroids at any temperature, IBc-grafted silica stationary phases were able to resolve steroid peaks with increasingly retarded retention times in correlation to both increased BMA content and increased temperature. (wikipedia.org)
  • liquid
  • The most common method of measuring amino acid hydrophobicity is partitioning between two immiscible liquid phases. (wikipedia.org)