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  • molecules
  • The molecules are separated by size due to the gel filtration mechanism and by electrophoretic mobility due to the gel electrophoresis mechanism. (wikipedia.org)
  • Although Helmchen's postulates are specific for amides on silica gel using liquid chromatography, the postulates provide fundamental guidelines for other molecules. (wikipedia.org)
  • The effect originates from the disruption of highly dynamic hydrogen bonds between molecules of liquid water. (wikipedia.org)
  • This process creates a competitive interaction between the desired protein and the immobilized stationary molecules, which eventually lets the now highly purified proteins be released. (wikipedia.org)
  • The other molecules in the mobile phase will not become trapped as they do not possess this property. (wikipedia.org)
  • The water-soluble and charged molecules such as proteins, amino acids, and peptides bind to moieties which are oppositely charged by forming ionic bonds to the insoluble stationary phase. (wikipedia.org)
  • The equilibrated stationary phase consists of an ionizable functional group where the targeted molecules of a mixture to be separated and quantified can bind while passing through the column-a cationic stationary phase is used to separate anions and an anionic stationary phase is used to separate cations. (wikipedia.org)
  • elution
  • There are also instances of the elution times increasing below 15 °C, which most likely can be attributed to the lower temperatures' effects on mass transfer playing a more significant role on retention than the stationary phase behavior. (wikipedia.org)
  • The elution buffer disrupts interactions between the bound target biomolecules with the stationary to a much greater extent than the wash buffer, effectively removing the target biomolecules. (wikipedia.org)
  • Highly specific elution of the desired macromolecule from the stationary phase is usually effected by adding to the eluting buffer a gradient of the same kind on the macromolecule and displaces it. (wikipedia.org)
  • Because of the abundant separating columns, elution systems, and detectors available, chromatography has developed into the main method for ion analysis. (wikipedia.org)
  • 1975
  • Not until 1975 was "ion chromatography" established as a name in reference to the techniques, and was thereafter used as a name for marketing purposes. (wikipedia.org)
  • detectors
  • It was in the fifties and sixties that theoretical models were developed for IC for further understanding and it was not until the seventies that continuous detectors were utilized, paving the path for the development from low-pressure to high-performance chromatography. (wikipedia.org)
  • capillaries
  • An organically modified silica glass material containing octyl groups (C 8 ) was fabricated by the sol-gel process and coated onto the inner walls of fused silica capillaries. (springer.com)
  • hydrophobic
  • Where pure PNIPAAm was unable to resolve hydrophobic steroids at any temperature, IBc-grafted silica stationary phases were able to resolve steroid peaks with increasingly retarded retention times in correlation to both increased BMA content and increased temperature. (wikipedia.org)