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  • Protein
  • Stat1 interacts with P53 TUMOR SUPPRESSOR PROTEIN and regulates expression of GENES involved in growth control and APOPTOSIS. (nih.gov)
  • To this end, we presently evaluated the role of the transcription factor CCAAT/enhancer binding protein delta (C/EBPδ) on β-cell apoptosis and production of inflammatory mediators in the rat insulinoma INS-1E cells, in purified primary rat β-cells and in human islets. (nih.gov)
  • Examples include the NLSs of the influenza virus NP protein ( 55 ), the Borna disease virus p10 protein ( 62 ), the cellular transcription factor Stat1 ( 47 , 34 , 35 ), the histone deacetylase 4 ( 18 , 54 ), and the U1A spliceosome protein ( 22 ). (asm.org)
  • We focus on how the retinoblastoma protein and its downstream target E2F transcription factor mediate proliferation, differentiation and apoptosis in response to specific signaling cascades. (moffitt.org)
  • To find relevant epigenetic signatures enriched at the ARA-lincRNAs' transcription start sites (TSSs) we used a machine learning approach and identified that the ARA-lincRNA genomic loci in LNCaP cells are significantly enriched with epigenetic marks that are characteristic of in cis enhancer RNA regulators, and that the H3K27ac mark of active enhancers is conspicuously enriched at the TSS of ARA-lincRNAs adjacent to androgen-activated protein-coding genes. (frontiersin.org)
  • mRNA
  • Closer examination revealed that SOCS-3 but not SOCS-1 mRNA levels increase in an RNA- and nuclear factor kappa B (NF-kappaB)-dependent but type I IFN-independent manner early in the viral replication cycle. (nih.gov)
  • The expression of mRNA specific for the NK-associated transcription factor Id2 and for Bcl-2 and GATA-3 was investigated by quantitative PCR (qPCR) in freshly isolated (day 0) and cytokine-differentiated CD34-lineage- cells (week +4), as previously detailed . (nih.gov)
  • regulation
  • C/EBPδ deficiency increased the up-regulation of the transcription factor CHOP in response to cytokines, enhancing expression of the pro-apoptotic Bcl-2 family member BIM. (nih.gov)
  • The regulation of transcription is of utmost interest as it is central not only to developmental biology, but also to cancer biology, drug screening, etc. (biochemsoctrans.org)
  • These loss of function studies demonstrate an essential role of endogenous IFN and activated STAT1 for constitutive MHC class I expression in normal mice and define IL-7-dependent but IFN-independent regulation of STAT1 restricted to T lymphocytes. (nih.gov)
  • Cytokines
  • The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3), and research investigators consider it an important target in the diagnosis and treatment of cancer (4). (cellsignal.com)
  • genetic
  • Raimondi S, Maisonneuve P, Lohr JM, Lowenfels AB: Early onset pancreatic cancer: evidence of a major role for smoking and genetic factors. (springermedizin.de)
  • utilize
  • Signal transducers and activators of transcription (STAT) utilize karyopherins-alpha (KPNA) for their passage from cytoplasm into the nucleus. (biomedcentral.com)
  • By describing known factors which affect expression estimates this article provides guidelines for appropriate quality control and pre-processing of the data, additionally applicable to other transcriptome analysis methods that utilize similar sample handling protocols. (beds.ac.uk)
  • expression
  • Gene regulatory network analysis highlights overrepresented and deregulated HNF4 and CAR (Cluster 1), Krüppel-like factors MafF and ELK1 (Cluster 2) as well as ETF (Cluster 3) among the interspecies conserved key regulators of expression changes. (springer.com)
  • Surprisingly, an IFN-independent requirement for STAT1 was also found, specifically in T lymphocytes, where MHC class I expression was not fully accounted for by IFN signaling. (nih.gov)
  • Reduced surface expression of MHC class I antigens in different subsets of STAT1−/− lymphocytes. (nih.gov)
  • Additionally, we describe methods that can be used to estimate the influence of these factors, and to control the way in which they affect the expression estimates. (beds.ac.uk)
  • analysis
  • We then performed a multivariate analysis on all the factors collectively. (gersteinlab.org)
  • Analysis of microarray data is however very complex, requiring sophisticated methods to control for various factors that are inherent to the procedures used. (beds.ac.uk)
  • regions
  • Moreover, BoCaTFBS differs from most other motif programs in that (1) it takes into consideration positional dependencies within a given motif and (2) it uses the negative data (regions where the transcription factor does not bind) in order to refine the binding site. (gersteinlab.org)
  • human
  • To explore embryonic functions of STAT1, we investigated stat1b, an unstudied zebrafish co-ortholog of human STAT1 . (zfin.org)
  • specific
  • This grouped the transcription factors into sequence-specific and sequence-nonspecific clusters. (gersteinlab.org)
  • To obtain significant results, microarray data need to undergo statistical processing to differentiate between signal changes caused by direct experimental factors and arising from the indirect experimental factors such as specific methods used, as well as from inaccuracies of the measurements. (beds.ac.uk)
  • important
  • In this article we describe the individual steps of a microarray experiment, highlighting important elements and factors that may affect the processes involved and that influence the interpretation of the results. (beds.ac.uk)