Staphylococcus phages. Medical search. Frequent questions

However, successful therapy requires understanding the genetic basis of host range-the subset of strains in a species that could be killed by a particular phage. (robertpetit.com)
However, phage resistance genes that were not part of the core genome were significantly less consistent with the core genome phylogeny than all noncore genes in this set, suggesting more frequent movement between strains by horizontal gene transfer. (robertpetit.com)
TSS is caused by toxin-producing strains of staphylococci (staphylococcal TSS) and streptococci (streptococcal TSS). (medscape.com)
Firstly, lysogenic phages are natural vectors that have evolved to integrate and propagate their DNA through specific bacterial strains. (igem.org)
Lysogenic phages have evolved to insert their DNA into the genomes of specific strains of bacteria. (igem.org)
Herein we report the identification and characterisation of two linear antimicrobial peptides (AMPs), HG2 and HG4, with activity against a wide range of multi-drug resistant (MDR) bacteria, especially methicillin resistant Staphylococcus aureus (MRSA) strains, a highly problematic group of Gram-positive bacteria in the hospital and community environment. (microbiologyresearch.org)
Distribution of coagulase positive, phage typable Staphylococcus aureus strains and predominant microbial types in throat, nasal, skin and aerosol smaples were determined. (dtic.mil)
All 28 MRSA strains (27 bovine and 1 human) produced PBP2a, according to latex agglutination test (Oxoid Ltd.). Eight of 27 randomly chosen bovine strains and the human strain containing the mec A gene, as well as 4 bovine and 2 human mec A-negative isolates, were phage typed with MRSA phages ( 6 ) at the Institute of National Public Health and Medical Officers Service in Hajdú-Bihar County. (cdc.gov)
None of the MRSA or methicillin-susceptible S. aureus (MSSA) strains were lysed by phages in RTD. (cdc.gov)
All MRSA strains showed a similar lysis pattern with the 100× RTD MRSA phages ( Table ). (cdc.gov)
Staphylococcal scalded skin syndrome (SSSS) is a rare dermatological condition associated with extensive desquamation by toxicogenic strains of staphylococcus aureus. (amrita.edu)
Do the oral Staphylococcus aureus strains from denture wearers have a greater pathogenicity potential? (growkudos.com)
They then conducted laboratory experiments to test which bacteria these phages infect, using nearly 120 bacterial strains, covering 29 staphylococcal species. (nfp72.ch)
They systematically assessed phage resistance evolution and bacterial fitness in Pseudomonas , including sequencing more than 250 mutant strains! (phagedirectory.com)

Bacteriophages (green, red) infecting Staphylococcus aureus cells (black clusters). (rockefeller.edu)
Here we describe our studies on the genetic organization of phages infecting Staphylococcus aureus, a pathogen of human importance, by using an assembly of tools for gene annotation, identification of expression components, and phylogeny analysis. (bvsalud.org)

When Staphylococcus aureus bacteria are not causing pneumonia or idling harmlessly in our nostrils, they are busy fighting their own battles against a constant onslaught of phage virus bent on infecting them. (rockefeller.edu)
Curious about how this non-specific system impacts the host bacteria, Marraffini and Mo exposed Staphylococcus aureus cells to phage and watched the type III system come online. (rockefeller.edu)
It is plausible, Marraffini says, that mutations caused by type III CRISPR are among the major factors in the transformation of harmless Staphylococcus aureus bacteria into more deadly antibiotic-resistant variants. (rockefeller.edu)
Phages that infect multiple bacteria can ferry beneficial genes between hosts, but only if they manage to do so before they are eradicated by CRISPR. (rockefeller.edu)
Marraffini suspects that the mutations introduced by DNA repair offset that loss-ensuring that, although the opportunity for horizontal gene transfer via phage is lost, bacteria still have a chance to mutate and benefit from further evolution. (rockefeller.edu)
Then transform the plasmid into the host bacterial strain and expose the bacteria to the phage. (igem.org)
Despite phage replication in these matrices, lysis of the bacteria was slower than phage propagation, and no reduction of the bacterial population was observed. (bvsalud.org)
Phages kill bacteria. (nfp72.ch)
Bacteriophages (short: phages) are viruses that infect and kill bacteria. (nfp72.ch)
But phages not only kill bacteria, they can also transfer antibiotic resistance genes from one bacterium to another: When multiplying in the cells of targeted bacteria, they inject their own genetic material into new virus particles, and in some cases also add genetic material from the host bacteria - sometimes, this can be a resistance gene. (nfp72.ch)
Up to now, it was widely assumed that specific phages can only infect very specific bacteria. (nfp72.ch)
They found these in wastewater, where a great diversity of bacteria and their phages converge - from humans, animals, households, industry and agriculture. (nfp72.ch)
Furthermore, the teams' findings are crucial regarding the use of phages to combat pathogenic bacteria in humans. (nfp72.ch)
The discovery that phages can have a broad host range makes it easier to use them against many different bacteria. (nfp72.ch)
Xin You (Helmholtz Centre for Environmental Research, Germany) and colleagues published a preprint on phage co-transport with hyphal-riding bacteria . (phagedirectory.com)
They report the ability of hyphal-riding bacteria to transport lytic phages and utilize them as 'weapons' during colonization of new water-unsaturated habitats. (phagedirectory.com)
The Silveira Lab at the University of Miami, Florida is hiring a Postdoctoral Associate to study the tripartite relationships between phage-bacteria interactions in coral reef. (phagedirectory.com)
phages are viruses that can infect bacteria, just like a coronavirus and the flu virus can infect us, and usually the phages kill the bacteria. (cdc.gov)
But some phages can also infect bacteria without killing them. (cdc.gov)

We found that genes encoding biosynthesis of molecules that were targets of S. aureus phage adsorption to the outer surface of the cell were the most conserved in the pangenome. (robertpetit.com)
Taken together, these results suggested that, while phage adsorption genes are heavily conserved in the S. aureus species, HGT may play a significant role in strain-specific evolution of host range patterns. (robertpetit.com)
The inhibition of the phages associated with a reduction in phage adsorption, quantified to 99% at 10% plasma. (bvsalud.org)
Jennifer Mahony (University College Cork, Ireland) published a commentary in mSystems on the use of cell surface polysaccharides as a common strategy for phage adsorption . (phagedirectory.com)

We describe the design and preclinical development of AB-SA01, a fixed-composition bacteriophage product intended to treat Staphylococcus aureus infections. (pacb.com)
Bacteriophage (phage) attachment to the host bacterial surface is an important process during phage infection, which involves interactions between phage receptor-binding proteins and host receptor molecules. (elsevierpure.com)
Here we show that staphylococcal phages produce a structured RNA transcribed from the terminase subunit genes, termed CBASS-activating bacteriophage RNA (cabRNA), which binds to a positively charged surface of the CdnE03 cyclase and promotes the synthesis of the cyclic dinucleotide cGAMP to activate the CBASS immune response. (bvsalud.org)

Wolfram-Schauerte M, Pozhydaieva N, Viering M, Glatter T, Höfer K. (2022) Integrated Omics Reveal Time-Resolved Insights into T4 Phage Infection of E. coli on Proteome and Transcriptome Levels. (spp2330.de)

In 1956, Kaesberg and his colleagues studied staphylococcus bacteriophages via electron microscopy and found that freeze-dried particles and air-dried particles had significant morphological differences, specifically in size and shape. (wikipedia.org)
The propagation of bacteriophages and other mobile genetic elements requires exploitation of the phage mechanisms involved in virion assembly and DNA packaging. (unavarra.es)

Only superinfection immunity genes encoded by temperate phages inserted in the genome correlated with experimentally determined temperate phage resistance. (robertpetit.com)
The objectives of our phage standard include negating the issues of genome size, exploiting phage characteristics for use as a vector, and developing a BioBrick compatible standard applicable to all lysogenic phages. (igem.org)
The phage standard describes the process of adding a given Biobrick part, which we will call source DNA into the genome of a lysogenic phage, referred to as host DNA . (igem.org)
This will require secondary DNA sequences including the phage genome integration site , some garbage DNA (flanked by essential restriction enzyme cut sites) and the low-copy number BioBrick plasmid . (igem.org)
The first step is to choose restriction enzymes using webcutter 2.0 that appear only once , closest to the region of the phage genome that is going to be modified (Fig. 1). (igem.org)
Current state of the low copy BioBrick plasmid containing the full phage genome. (igem.org)
Cut and ligate it all back together and viola, a modified phage genome is at your disposal. (igem.org)
Kupczok A , Bailey ZM, Refardt D, Wendling CC. Co-transfer of functionally interdependent genes contributes to genome mosaicism in lambdoid phages. (spp2330.de)
Pawel Markwitz (University of Wroclaw, Poland) and colleagues published a new paper in ISME on genome-driven elucidation of phage-host interplay and the impact of phage resistance evolution on bacterial fitness . (phagedirectory.com)
Draft genome sequence of Staphylococcus aureus 118 (ST772), a major disease clone from India. (ccamp.res.in)
Draft genome sequence of Staphylococcus aureus ST672, an emerging disease clone from India. (ccamp.res.in)
Genome sequencing unveils a novel sea enterotoxin-carrying PVL phage in Staphylococcus aureus ST772 from India. (ccamp.res.in)

In this in vitro and animal study, we demonstrated that the activity of anti-staphylococcal phages is severely impaired in 0.5% plasma or synovial fluid. (bvsalud.org)
Staphylococcus aureus pathogenicity islands (SaPIs) have an intimate relationship with temperate staphylococcal phages. (unavarra.es)

These results strongly suggest that ORF16 of phage S24-1 and its homologs may be the receptor-binding proteins of AHJD-like viruses. (elsevierpure.com)
Cyclic oligonucleotide-based antiphage signalling systems (CBASS) protect prokaryotes from viral (phage) attack through the production of cyclic oligonucleotides, which activate effector proteins that trigger the death of the infected host1,2. (bvsalud.org)

They're looking to publish novel phage bioinformatics tools, AI in phage biology and therapy, and tools for host discovery and prediction, phage characterization, database tools, and more! (phagedirectory.com)

In vitro, AB-SA01 killed 94.5% of 401 clinical Staphylococcus aureus isolates, including methicillin-resistant and vancomycin-intermediate ones for a total of 95% of the 205 known multidrug-resistant isolates. (pacb.com)
Surface swabs were tested for lytic phages and bacterial isolates for mitomycin C-inducible prophages. (mdpi.com)
Isolates from the stuffed chicken breasts and from stool cultures from two ill persons were positive for S. aureus phage type 53. (cdc.gov)
The isolates initially characterized as staphylococci were tested for coagulase production (in tubes) and with Slidex Staph Plus test (bioMérieux, Marcy l'Etoile, France) to confirm their identification as S. aureus . (cdc.gov)

We collected 77 environmental samples from the surroundings of 12 patients with infection/colonizations by Escherichia coli , Staphylococcus aureus or Klebsiella spp. (mdpi.com)

Phage therapy has been proposed as a possible alternative treatment for infections caused by the ubiquitous bacterial pathogen Staphylococcus aureus . (robertpetit.com)
It is exciting that we found this in a major human pathogen, Staphylococcus aureus , one known to develop antibiotic resistance. (rockefeller.edu)
S. aureus is a pathogen that frequently causes severe morbidity and phage therapy is being discussed as an alternative to antibiotics for the treatment of S. aureus infections. (bvsalud.org)

This will allow the phage to infect the bacterial cells as well as the integration site on the low-copy BioBrick plasmid. (igem.org)

Toxic shock syndrome (TSS) caused by methicillin-resistant Staphylococcus aureus (MRSA) nosocomial infection is a growing concern in both adult and pediatric patients. (medscape.com)
How bacterial cyclases recognize phage infection is not known. (bvsalud.org)
Consistent with these in vitro findings, phage treatment did not reduce bacterial burdens in a neutropenic mouse S. aureus thigh infection model. (bvsalud.org)
Multiple phage resistance systems inhibit infection via SIR2-dependent NAD + depletion. (spp2330.de)
In through the Out Door: A Functional Virulence Factor Secretion System Is Necessary for Phage Infection in Ralstonia solanacearum. (ulaval.ca)

2021. Analysis of viromes and microbiomes from pig fecal samples reveals that phages and prophages rarely carry antibiotic resistance genes. (ulaval.ca)
2021. Phage Cocktail Development against Aeromonas salmonicida subsp. (ulaval.ca)

AB-SA01 contains three naturally occurring, obligately lytic myoviruses related to Staphylococcus phage K. AB-SA01 component phages have been sequenced and contain no identifiable bacterial virulence or antibiotic resistance genes. (pacb.com)
Putative phage resistance genes that were core components of the pangenome genes had similar nucleotide diversity, ratio of nonsynonymous to synonymous substitutions, and functionality (measured by delta-bitscore) to other core genes. (robertpetit.com)
In Staphylococcus aureus, the phage-mediated acquisition of mobile genetic elements (MGEs) that encode virulence and antibiotic resistance genes largely contribute to its evolutionary adaptation and genetic plasticity. (bvsalud.org)
Staphylococcus aureus is one of the most relevant mastitis pathogens in dairy cattle, and the acquisition of antimicrobial resistance genes presents a significant health issue in both veterinary and human fields. (bvsalud.org)
On the other hand, however, when using phages in medicine, one must be careful that they don't unintentionally transmit antibiotic resistance genes. (nfp72.ch)
Genes Influencing Phage Host Range in Staphylococcus aureus on a Species-Wide Scale. (cdc.gov)

The spontaneous frequency of resistance to AB-SA01 was =3 × 10-9, and resistance emerging to one component phage could be complemented by the activity of another component phage. (pacb.com)
But occasionally a fortuitous mutation can confer resistance to other phages, or even antibiotics. (rockefeller.edu)
However, this mechanism has received little attention regarding the spread of antibiotic resistance to date, and little is known about the role phages - the most abundant biological entities on earth - play in the spread of antibiotic resistance across humans, animals and the environment. (nfp72.ch)
This would narrow their scope of impact and act as a natural barrier for the phage-mediated spread of genetic material, and thus antibiotic resistance. (nfp72.ch)
These results prove that phages create widely branched connections through which antibiotic resistance can spread between bacterial species, across the entire biological system of humans, animals and the environment. (nfp72.ch)
They examined bacterial resistance mechanisms, the current state of phage therapy, how phage therapy can augment strategies to combat resistance, and the role of efflux pumps in antimicrobial resistance. (phagedirectory.com)

Staphylococcus aureus is a clinically important bacterium that is commensal in both humans and animals. (elsevierpure.com)
S. aureus is known to bind multiple coagulation factors, resulting in the formation of aggregates and blood clots that might protect the bacterium from the phages. (bvsalud.org)
Phages attack a bacterium (illustration). (nfp72.ch)
2023. CRISPR-Cas provides limited phage immunity to a prevalent gut bacterium in gnotobiotic mice. (ulaval.ca)

D. Entry to strong phage preparations To boost the newest proportion out of typable societies past three-household, Williams and you may Rippon (1952) re-checked-out having undiluted phages those individuals countries and that provided zero strong responses from the RTD. (rcv-led.ca)

First, based on a comparative genomic analysis of phages S24-1 and S13′, open reading frame 16 (ORF16) of phage S24-1 was speculated to be the receptor-binding protein, which possibly determines the host range. (elsevierpure.com)

In our July PHAVES series, Dr. Ameneh Khatami, a paediatric infectious disease physician and senior lecturer at the University of Sydney, spoke about phage therapy for difficult to treat infections in children. (phagedirectory.com)

However, little information is available on the receptor-binding protein of S. aureus phages. (elsevierpure.com)
In the present study, we investigated the receptor-binding protein of AHJD-like viruses using phage S24-1. (elsevierpure.com)
Second, we demonstrated that this was the receptor-binding protein of phage S24-1. (elsevierpure.com)
M13 phage displaying an in vivo biotinylatable peptide (AviTag) genetically fused to the phage tail protein pIII were used as reporter particle scaffolds, with biotinylated aptamers attached via avidin-biotin linkages, and horseradish peroxidase (HRP) reporter enzymes covalently attached to the pVIII coat protein. (nih.gov)
These modified viral nanoparticles were used in immunochromatographic sandwich assays for the direct detection of IgE and of the penicillin-binding protein from Staphylococcus aureus (PBP2a). (nih.gov)

Temporal trends of livestock-associated methicillin-resistant Staphylococcus aureus infections in Denmark. (cdc.gov)
Design and Preclinical Development of a Phage Product for the Treatment of Antibiotic-Resistant Staphylococcus aureus Infections. (pacb.com)
In summary, phage treatment of S. aureus infections inside the body may be fundamentally challenging, and more investigation is needed prior to proceeding to in-human trials. (bvsalud.org)

S. aureus virulent phages S24-1 and S13′ (family Podoviridae, genus AHJD-like viruses) were isolated from sewage. (elsevierpure.com)

With the burst of publications on phage isolation and genetic studies during the last decade, several different bioinformatics programs have been used. (bvsalud.org)

Phage-mediated transfer of microbial genetic elements plays a crucial role in bacterial life style and evolution. (unavarra.es)
The researchers observed numerous different combinations of targets, so that "shared" phages may enable one staphylococcal species to exchange genetic material with, on average, seventeen other species. (nfp72.ch)

Drug developers thus have to pay close attention to the exact propagation mechanism of phages used in medicine. (nfp72.ch)

Typable societies following essentially meet or exceed 90%, however the learning off reactions gotten having phages on RTD x a lot of presents dilemmas (Sections J K D , 2 and 1V.C). Hence the effectiveness of phage for secondary entering is after less to RTD x one hundred (Statement, 1971). (rcv-led.ca)

Examples of research areas include: Gene replacement using CRISPR, Viral delivery of transgenes, or creation of synthetic phages to target and kill drug resistant pathogens. (phagedirectory.com)

Third, our study suggested that wall teichoic acids in the cell walls of S. aureus are the main receptor molecules for ORF16 and phage S24-1. (elsevierpure.com)

Overall, the inherent characteristics of AB-SA01 component phages meet regulatory and generally accepted criteria for human use, and the preclinical data presented here have supported production under good manufacturing practices and phase 1 clinical studies with AB-SA01. (pacb.com)
While Staphylococcus aureus (the best-known species of the genus) has been for long subject of studies on this form of gene transfer, research has primarily worked with samples from human medicine and identified phages that are of immediate clinical relevance. (nfp72.ch)
For PHAVES #22 next week, Dr. Saima Aslam, MD (IPATH, UCSD, California) will present 'Clinical phage therapy: Learning from patients' on Sept 20 at 9AM Pacific! (phagedirectory.com)

We isolated methicillin-resistant Staphylococcus aureus (MRSA) from cows with subclinical mastitis and from a person who worked with these animals. (cdc.gov)

Olivia Williams Barber (Northwestern University, USA) and colleagues published a minireview in mSystems on how the future of phage therapy will promote antimicrobial susceptibility . (phagedirectory.com)

Since 2007, phages have been used sporadically to treat bacterial infections in well-defined cases in the Queen Astrid military hospital (QAMH) in Brussels, Belgium. (mdpi.com)
And we actually have exploited that behavior since 1919, when we started to use phage therapy to treat bacterial infections. (cdc.gov)

Phage Australia is a national network of phage researchers and clinician scientists who aim to professionalise phage therapy as the third major intervention for infectious diseases, after vaccines and antibiotics. (phageaustralia.org)

It turned out that phages also incorporated this into new virus particles. (nfp72.ch)

Contrary to previous assumptions, it was shown that phages can attack not only one bacterial species, but on average four different ones. (nfp72.ch)

There is a growing interest in phage therapy as a complementary tool against antimicrobial resistant infections. (mdpi.com)
In the last two years, external requests for phage therapy have increased significantly. (mdpi.com)
From April 2013 to April 2018, 260 phage therapy requests were addressed to the QAMH. (mdpi.com)
Of these 260 requests, only 15 patients received phage therapy. (mdpi.com)
Streptococcal TSS is similar to that caused by Staphylococcus aureus , but mortality is higher (20 to 60%) despite aggressive therapy. (msdmanuals.com)

This position will use cryo-electron microscopy combined with functional techniques to study the mechanisms of transport across biological membranes, including phages. (phagedirectory.com)

Novel staphylococci nucH taxonomical marker used in identification of human‐associated Staphylococcus succinus subsp. (growkudos.com)

Staphylococcal superantigen-carrying pathogenicity islands (SaPIs) are discrete, chromosomally integrated units of ∼15 kilobases that are induced by helper phages to excise and replicate. (unavarra.es)

Lastly, lysogenic phage genomes are too large to be manipulated using normal BioBrick plasmids. (igem.org)
For our project, these insertion vectors will be low-copy BioBrick plasmids containing the integration site of a given phage, flanked by chosen restriction sites. (igem.org)

An in-depth analysis of phage genomic sequences is essential for the proposal of a cocktail for therapeutic uses. (bvsalud.org)

Characterisation of a Staphylococcus aureus Isolate Carrying Phage-Borne Enterotoxin E from a European Badger ( Meles meles ). (bvsalud.org)
The researchers were able to isolate almost a hundred different types of phages from wastewater for their project. (nfp72.ch)

Anatomy6

Organisms34

Diseases16

Chemicals and Drugs56

Analytical, Diagnostic and Therapeutic Techniques and Equipment14

Phenomena and Processes26

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture2

Information Science3

Health Care3

Strains14

Infecting Staphylococcus aureus2

Bacteria19

Adsorption4

Bacteriophage3

20221

Bacteriophages2

Genome12

Staphylococcal phages2

Proteins2

Characterization1

Isolates4

Escherichia1

Pathogen3

Infect1

Infection5

20212

Genes6

Resistance6

Bacterium4

Strong phage1

Genomic analysis1

Ameneh Khatami1

Protein5

Aureus infections3

Viruses1

Isolation1

Genetic2

Propagation1

Typable1

Pathogens1

Wall teichoic1

Clinical3

Mastitis1

Susceptibility1

Treat bacterial infections2

Antibiotics1

Particles1

Species1

Therapy5

Biological1

Subsp1

Pathogenicity1

Plasmids2

Sequences1

Isolate2