Staphylococcal protein a. Medical search. Frequent questions

Therefore, to challenge the feasibility of this approach, we used the Staphylococcus aureus alkaline shock protein 23 (Asp23) as a model to gain a deeper insight into the spatial organization of Asp23 within the cell. (nature.com)
Our aim was to provide information about the production of Egg White Immunoglobulin (EWIg) with specificity to Staphylococcal protein-A , a surface antigen of Staphylococcus aureus and to study the inhibition of this bacterium growth in pre- and post-immunized hens. (omicsonline.org)
Protein A is a cell wall protein deriving from Staphylococcus aureus which exhibits unique binding properties for IgG from a variety of mammalian species and for some IgM and IgA as well. (abeomics.com)
A specific purification process with strict quality control was taken to get the recombinant protein A with the purity of more than 98% , no human IgG affinity step is used during validated fermentation and purification and devoid of bacterial contaminant found normally in native Protein A. (Free of Staphylococcus endotoxins and hemolysin). (abeomics.com)
Staphylococcal Scalded Skin Syndrome (SSSS) is caused by a special type of Staphylococcus aureus (S.aureus) which can produce exfoliative toxins. (springer.com)
Recombinant protein of Staphylococcus aureus protein A with proprietary mutation. (eliteimmunebioservices.com)
In this study, using a combination of the bioinformatics design and molecular genetics, the protein A signal peptide from Staphylococcus aureus was modified, redesigned and then fused to the mature hGH coding region. (biomedcentral.com)
The ability to clot blood by producing coagulase distinguishes the virulent pathogen, Staphylococcus aureus , from the less virulent coagulase-negative staphylococcal species. (merckmanuals.com)
Staphylococcus aureus is a common cause of staphylococcal food poisoning in Australia with several outbreaks associated with foods prepared by commercial caterers. (who.int)

Mobile Unnatural Amino Acid Side Chains in the Core of Staphylococcal Nuclease. (atomistry.com)
To examine the role of water penetration, we have measured the 17O and 2H magnetic relaxation dis- persions (MRD) for the V66E and V66K mutants of staphylococcal nuclease, where glutamic acid and lysine residues are buried in predominantly apolar environments. (lu.se)

Whole blood collected from each participant was incubated for 18 hours with and without two microbial stimulants (lipopolysaccharide/LPS and staphylococcal enterotoxin type B/SEB) using TruCulture technology to evaluate immune cell activity. (cdc.gov)
Gupta DD, Saha S, Chakrabarti MK (2005) Involvement of protein kinase C in the mechanism of action of Escherichia coli heat-stable enterotoxin (STa) in a human colonic carcinoma cell line, COLO-205. (springer.com)
Foods high in starch and protein are believed to favour staphylococcal enterotoxin (SE) production. (who.int)

Higher incidence was found in developing countries where the incidence of staphylococcal infections is higher [ 8 ]. (springer.com)
For treatment of staphylococcal and streptococcal infections. (medscape.com)
Clindamycin is a lincosamide for the treatment of serious skin and soft tissue staphylococcal infections. (medscape.com)
Staphylococcal infections are of major importance in both human and veterinary medicine. (frontiersin.org)

Due to the close relationship of animals with the environmental microbiome and resistome, animal staphylococcal strains also represent a source of resistance determinants. (frontiersin.org)
The coagglutination test, which uses staphylococcal protein A, for serotyping strains of Streptococcus pneumoniae, was extended to include serotyping within serogroups. (bmj.com)
In conclusion, we show that this multiplex competitive Luminex assay is a suitable method for the rapid and simultaneous determination of S. aureus IgG-accessible cell-surface-associated proteins and carbohydrates in various growth phases and growth conditions in different S. aureus strains. (eur.nl)
Excess and inappropriate use of clindamycin have led to the emergence of resistant Staphylococcal strains. (clin-lab-publications.com)

The mechanism of resistance usually involves modification of normal or the presence of acquired PENICILLIN BINDING PROTEINS. (bvsalud.org)

SCOP: Structural Classification of Proteins and ASTRAL. (berkeley.edu)
SCOPe: Structural Classification of Proteins - extended. (berkeley.edu)

Recombinant Protein A was developed to increase the specificity of the molecule for IgG and is widely used both in research and bioprocessing. (abeomics.com)
Because a uniform dielectric ionizable group in a protein relative to the pKa value of the continuum model is a very crude description of the highly same group in a small reference molecule in dilute aqueous heterogeneous protein interior, the dielectric coefficient, eP, solution. (lu.se)

The ionizable amino acid side chains of proteins are usually located at the surface. (lu.se)

A sandwich Enzyme-Immunosorbent Assay (ELISA) showed a large concentration of anti-SpA antibodies in the eggs from hens immunized with protein A. The titer of these antibodies was at least 5 to 6-folds of that of the eggs from pre-immunized hens 10 days post-immunization. (omicsonline.org)
Grouping of beta-haemolytic streptococci with group-specific antibodies absorbed to staphylococcal protein A. (bmj.com)
Anti-protein C or S antibodies bind to protein C or S. Cross-reacting immunoglobulin G (IgG) autoantibodies increase protein S clearance from the circulation and are thought to trigger postinfectious PF. (logicalimages.com)

The bifunctional fusion protein was expressed in Escherichia coli, recovered on a cellulose column and purified by elution at alkaline pH, ProtA-CBD was used to purify IgG from rabbit serum and its ability to bind IgC from different sources was determined. (huji.ac.il)

Newborn innate immunity is distinct demonstrating diminished skin integrity, impaired Th1-polarizing responses, low complement levels, and diminished expression of plasma antimicrobial proteins and peptides, especially in preterm newborns. (harvard.edu)

Its activation is tightly controlled by numerous other signaling proteins including protein kinase C (PKC), Sab/SH3BP, and caveolin-1. (medscape.com)

Antibody is purified using immobilized protein A/G. (eliteimmunebioservices.com)
The antibody can be used as primary antibody in a capture ELISA assay against engineered recombinant Staphylococcal protein A (SpA) that diminished Fc binding. (eliteimmunebioservices.com)
Because staphylococcal Protein A (ProtA) binds specifically to IgG, it has been used for many immunological manipulations, most notably antibody purification and diagnostics. (huji.ac.il)

Intriguingly, helical localization patterns were reported for many other fluorescently tagged bacterial proteins (e.g. chemoreceptors, secretion proteins, RNase E, and the chromosome partitioning protein SetB) but often the questions remains how well these observations reflect the true situation 6 . (nature.com)

Fluorescence-tags, commonly used to visualize the spatial distribution of proteins within cells, can influence the localization of the tagged proteins by affecting their stability, interaction with other proteins or the induction of oligomerization artifacts. (nature.com)
Taxonomic distribution of proteins containing IG domain. (embl.de)

Inhibits bacterial growth possibly by blocking dissociation of peptidyl tRNA from ribosomes, causing RNA-dependent protein synthesis to arrest. (medscape.com)
Mupirocin inhibits bacterial growth by inhibiting RNA and protein synthesis. (medscape.com)
Trouw L., Nilsson S., Goncalvez I., Landberg G. and Blom A. M. (2005) C4b-binding protein binds to necrotic cells and DNA, which limits DNA release and inhibits complement activation. (lu.se)

Analysis of virulence related gene expression and computational simulations of pivotal proteins involved in pathogenesis demonstrate that myricetin downregulates the saeR global regulator and interacts with sortase A and α-hemolysin. (nature.com)
His own research interests include the role of anaerobic bacteria in health and disease, structure/function of staphylococcal superantigens, and the molecular pathogenesis of staphylococcal toxic shock syndrome. (cdc.gov)

The recombinant protein A is produced by expressing a modified protein A gene in E.coli. (abeomics.com)
He has authored over 350 peer-reviewed papers and book chapters and is co-recipient of several US and international patents on the use of the 60 kDa heat shock protein (HSP60) gene sequences as universal targets for infectious disease diagnosis and microbial species identification. (cdc.gov)
Methicillin resistance is due to the acquisition of the mecA gene, that encodes a new protein designated PBP2a, belonging to a family of enzymes necessary in building the bacterial cell wall. (frontiersin.org)
We demonstrated that in an S. aureus srtA gene knockout strain, srtA-dependent cell-surface-associated proteins were detectable yet not covalently linked to the PG layer. (eur.nl)

Unlike most coagulase-negative staphylococcal species, S. lugdunensis often remains sensitive to penicillinase-resistant beta-lactam antibiotics (ie, methicillin-sensitive). (merckmanuals.com)
Encapsulated bacteria, most commonly Streptococcus pneumoniae, followed by Haemophilus influenzae type b and staphylococcal species, are the typical pathogens. (medscape.com)

Furthermore, Myr confers a significant degree of protection against staphylococcal infection in the Galleria mellonella model. (nature.com)
It can be due to inherited protein C deficiency or acquired protein C deficiency in the setting of severe bacterial infection, commonly Neisseria meningitidis . (logicalimages.com)
Characterization of distinct aspects of the neonatal immune response is defining novel approaches to enhance host defense to prevent and/or treat staphylococcal infection in this vulnerable population. (harvard.edu)

Urosepsis and staphylococcal sepsis are further common causes. (logicalimages.com)

Immediately after insertion host proteins are adsorbed to the surface of the implant. (lu.se)

In our previous work, we developed a six-point classification scoring schema with annotation pertaining to protein family scores, orthology, protein interaction/association studies, bidirectional best BLAST hits, sorting signals, known databases and visualizers which were used to validate protein interactions. (biomedcentral.com)

It produces a ring-shaped complex called a staphylococcal alpha-hemolysin pore. (wikipedia.org)
Hemolysin Proteins" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (harvard.edu)
This graph shows the total number of publications written about "Hemolysin Proteins" by people in Harvard Catalyst Profiles by year, and whether "Hemolysin Proteins" was a major or minor topic of these publication. (harvard.edu)
Below are the most recent publications written about "Hemolysin Proteins" by people in Profiles. (harvard.edu)

For example, it can have inhibitory effects on glycolysis followed by a direct effect on protein synthesis [ 2 ], as well as increase the absorption and retention of calcium, magnesium and phosphate ions in the body. (biomedcentral.com)
In the body, these toxins inhibit protein synthesis leading to cell death. (cdc.gov)

On the other hand, recombinant therapeutical proteins have received a great deal of attention in recent years due to their advantages including low side effects, minimized cytotoxicity, high selectivity, and very low non-specific interactions [ 7 , 8 ]. (biomedcentral.com)
Hydrophobicity was one of the most important physicochemical properties, due to the fact that it is involved in protein interactions, for example, by forming hydrophobic cores. (hindawi.com)
Bacterial toxins also catalyze the non-covalent modification of host protein function or can modify host cell properties through direct protein-protein interactions. (springer.com)
All or other nearby charged groups, the pKa shift of a buried pH-dependent properties of proteins are ultimately governed residue is dominated by the large electrostatic self-energy by the electrostatic interactions of ionizable side chains. (lu.se)
2004). The effect of electrostatic interactions is usually polarization effects in the protein are subsumed in an quantified in terms of the shift, DpKa, of the pKa value of an apparent dielectric constant, eP. (lu.se)
The results support the fact that a peptide as small as a hexamer can have specific interactions with large proteins such as hIgG. (duke.edu)

Applying this method, we show a cellular distribution of the staphylococcal alkaline shock protein 23 (Asp23), which is compatible with filament formation, a property of Asp23 that we also demonstrate in vitro . (nature.com)
In this work, we report the in vitro shuffling of two different modular domains using a protein self-assembly methodology. (korea.ac.kr)
In vitro self-assembly of functional domains from modular lytic enzymes on a protein scaffold likely expands the repertoire of bactericidal enzymes with improved activities. (korea.ac.kr)
This defect compromises multiple cell lineages and functions, and when protein expression is reestablished in-vitro, the Arp2/3 complex conformation and functions are rescued. (bvsalud.org)

Intoxication or staphylococcal food poisoning (SFP) occurs following ingestion of food products contaminated with heat-resistant S. aureus enterotoxins. (who.int)
Staphylococcal food poisoning (SFP) symptoms generally have a rapid onset, appearing within three hours after ingestion (range: 30 minutes to 6 hours). (who.int)

Computational design of novel proteins with well-defined functions is an ongoing topic in computational biology. (hindawi.com)
therefore, computational approaches combined with high-throughput experimental datasets are required to identify the function of proteins [ 9 , 14 ]. (biomedcentral.com)
Different computational methods have been designed for estimating protein function based on the information generated from sequence similarity, subcellular localization, phylogenetic profiles, mRNA expression profiles, homology modelling etc. [ 15 ]. (biomedcentral.com)

Since the native signal protein peptide of S. aureus protein A was not able to deliver hGH to the extracellular space, it was modified using bioinformatics tools and fused to the n-terminal region of hGh to show that the redesigned signal peptide was functional. (biomedcentral.com)

Sjöberg A., Trouw L., McGrath F., Hack E. C. and Blom A. M. (2006) Regulation of complement activation by C-reactive protein: targeting of the inhibitory activity of C4b-binding protein. (lu.se)

The production of recombinant mature hormone in suitable hosts and secretion of this therapeutic protein into the extracellular space can be considered as one of the best cost-effective approaches not only to obtain the active form of the protein but also endotoxin-free preparation. (biomedcentral.com)

Recent advances in genetic engineering have made it possible for microorganisms to be used for expression of heterologous proteins. (biomedcentral.com)
Here, we investigated expression and function of TREM-1 in a murine model of renal IR using different TREM-1 inhibitors: LP17, LR12 and TREM-1 fusion protein. (bvsalud.org)

Moreover, at 37 °C, the initial rate of cell lysis was over 3-fold higher than that with free Lst, thereby revealing the unique catalytic properties of the chimeric proteins. (korea.ac.kr)
More specifically, the team took the protein streptavidin, which acts as an effective template to which the researchers could attach a binding domain from one organism and a catalytic domain from another. (sciencedaily.com)
One of the methods that have been proposed is a multiobjective optimization, in which protein stability and catalytic activity are simultaneously optimized [ 4 , 5 ]. (hindawi.com)
Several methods have been proposed to design novel stable proteins, such as multi-objective optimization, in which protein stability and catalytic activity are simultaneously optimized. (hindawi.com)

Anti-GBM nephritis is a classic autoimmune disease characterized by the presence of circulating pathogenic autoantibodies directed against proteins in the glomerular and alveolar basement membranes. (medscape.com)

Immunoglobulin-like domains that are related in both sequence and structure can be found in several diverse protein families. (embl.de)

Bacterial toxins can be single proteins or oligomeric protein complexes that are organized with distinct AB structure-function properties. (springer.com)

Given the intimate relationship between a protein's structure and function, a way to design proteins with targeted properties is to start from a desired structure and find sequences able to fold into it, imposing additional constraints in the process [ 1 ]. (hindawi.com)

The colloidal gold was conjugated with recombinant streptococcal protein G (rSPG). (biomedcentral.com)
Blom A. M . , Berggård K., Webb J. H., Villoutreix B., Lindahl G. and Dahlbäck B. (2000) Human C4b-binding protein has overlapping but not identical binding sites for C4b and streptococcal M-proteins. (lu.se)

These known-unknown regions for which no functional links are discovered, i.e. those with no biochemical properties or obvious relatives in protein and nucleic acid databases are known as orphan genes, and the end products are called HPs [ 2 ]. (biomedcentral.com)
These proteins are of great importance, as many of them might be associated with human diseases, thus falling into functional families. (biomedcentral.com)

Hemolysins or haemolysins are lipids and proteins that cause lysis of red blood cells by disrupting the cell membrane. (wikipedia.org)
While most hemolysins are protein compounds, some are lipid biosurfactants. (wikipedia.org)

In this work, we generated and optimized a new synthetic fusion protein using an evolutionary approach. (hindawi.com)

is a group of inherited diseases in which epithelial adhesion protein defects lead to epithelial fragility and bullae formation. (msdmanuals.com)

Molecular modelling of Staphylococcal delta-toxin ion channels by restrained molecular dynamics. (ox.ac.uk)

Ig molecules are highly modular proteins, in which the variable and constant domains have clear, conserved sequence patterns. (embl.de)

Of these four proteins, from 16, 21, 38 and 52 kDa molecular weights, the 52 kDa protein had the highest affinity for Vn. (lu.se)
Methicillin-resistance is due to a penicillin-binding protein, which has a low affinity for beta-lactam antibiotics. (clin-lab-publications.com)

The strain, which adhesion was dependent on Vn was shown to express four binding proteins in its cell wall. (lu.se)

Transition metals are critical for all forms of life, with 30% of all proteins and 50% of all enzymes predicted to utilize a metal cofactor. (illinois.edu)

Recombinant Staphylococcal Protein A produced in E.Coli is a non-glycosylated, Polypeptide chain having a molecular mass of 45 kDa.Recombinant Staphylococcal Protein A is purified by proprietary chromatographic techniques. (abeomics.com)

Protein design methods use trial and error or more sophisticated methods like directed evolution or inverse folding to generate novel scaffolds or to find novel protein sequences folding into a defined scaffold, respectively. (hindawi.com)

Webb, J. H., Villoutreix, B. O., Dahlbäck, B. and Blom A. M. (2001) Localization of a hydrophobic binding site for protein S on the b-chain of C4BP. (lu.se)
Blom, A. M. , Rytkönen, A., Vasquez, P., Dahlbäck, B. and Johnsson, A-B. (2001) A novel interaction between type IV pili of Neisseria gonorrhoeae and complement regulatory protein - C4b-binding protein. (lu.se)
Blom A. M. , Kask L. and B. Dahlbäck (2001) Structural requirements for the complement regulatory activities of C4b-binding protein. (lu.se)

Myricetin affects both surface proteins and secreted proteins which indicate that its action is unrelated to inhibition of the agr quorum sensing system. (nature.com)
Most ionizable side chains reside at the protein surface, parent physical significance. (lu.se)
Section I. The Gram-Positive Cell Wall 1 -- Ultrastructure of Gram-Positive Cell Walls / Terry J. Beveridge 3 -- Surface Proteins on Gram-Positive Bacteria / Vincent A. Fischetti 11 -- Section II. (epa.gov)
Therefore, we developed a novel method for the rapid yet comprehensive characterization of S. aureus cell-surface-associated proteins and carbohydrates, based on a competitive Luminex assay. (eur.nl)
S. aureus specific IgGs from the serum are captured to the IgG-accessible staphylococcal antigens present on the bacterial surface, or in supernatant, in a dose dependent manner. (eur.nl)
The remaining non-captured IgGs are then quantified by incubation with Luminex beads to which the individual recombinant S. aureus surface proteins and carbohydrates are covalently linked. (eur.nl)

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