FluorescenceAuramine-rhodamineActinMycobacteriaCounterstainTuberculosisBacteriaFluorochrome dyeCytoplasmPropidium IodideZiehl-NeelsenNucleusSpecimenMycobacteriumDyesAssayTruantMethylene BlueGramReagentsEffluxEthidium BromideAcridineBiologicalNuclearNucleiConfocalImmunofluorescenceNocardiaDecolorizerPunctateMitochondriaMicroscopyHematoxylinMicroscopicCrystal VioletCollagenSmearInterpretationMembraneCommonly2020HoechstProteinsRinseCellsGlycogenBlueDetection
Fluorescence13
- The auramine-rhodamine stain (AR), also known as the Truant auramine-rhodamine stain, is a histological technique used to visualize acid-fast bacilli using fluorescence microscopy, notably species in the Mycobacterium genus. (wikipedia.org)
- There is no heat fixation done for the (Primary stain) Fluorescence dyes, unlike in the Ziehl Neelsen Staining method. (microbenotes.com)
- M. tuberculosis stained by fluorescence auramine-rhodamine. (microbenotes.com)
- Of the many bacteria studied only the acid-fast saprophytes took this stain, but their fluorescence was appreciably different in brightness and color from that of tubercle bacteria. (dtic.mil)
- DAPI - a fluorescent nuclear stain that is excited by ultraviolet light, showing blue fluorescence when bound to DNA. (microscopeworld.com)
- Rhodamine - a protein-specific fluorescent stain used in fluorescence microscopy. (microscopeworld.com)
- The cell viability and apoptosis were determined by CCK-8 assay and acridine orange/ethidium bromide (AO/EB) fluorescence staining assay. (greenmedinfo.com)
- Fluorescent staining of actin filaments in fixed tissue sections and tissue culture cells preparations.Note: Unlike many actin antibodies, Acti-stain™ 555 phalloidin binds only to F-actin resulting in low background fluorescence. (cytoskeleton.com)
- Absorbance and fluorescence scan of Acti-stain™ 555. (cytoskeleton.com)
- Above Brachionus manjavacas stained with Acridine orange and viewed in a fluorescence microscope 400X. (canadiannaturephotographer.com)
- Items that inherently fluoresce in the ultra-violet region will interfere with the dye stain fluorescence. (cbdiai.org)
- T.E.C. reportedly produces a brighter fluorescence of ridge detail with less interfeering background luminescence than other dye stains such as Rhodamine 6G and Ardrox. (cbdiai.org)
- Auramine stain binds to the mycolic acid present in the cell walls of acid-fast bacteria, causing them to emit a bright yellow-green fluorescence when exposed to ultraviolet light. (labtestsguide.com)
Auramine-rhodamine8
- Although the auramine-rhodamine stain is not as specific for acid-fast organisms (e.g. (wikipedia.org)
- The auramine-rhodamine staining technique being a histological type of stain that is used to stain and demonstrate the presence of Acid Fast-bacilli under a fluorescent Microscope and also known as Truant auramine-rhodamine stain, demonstrates the anatomy of the bacterial bacilli-cell. (microbenotes.com)
- The fluorochrome dye, Auramine-Rhodamine, used combine with the mycolic acid on the cell wall of the bacteria, which is then fixed by steamed heat. (microbenotes.com)
- Add enough quantity of the Auramine-Rhodamine Dyes (Flooding) on the smear and allow it to stand for 15 minutes and ensure the dyes stain the smear well. (microbenotes.com)
- Figure 2: Mycobacteria stained with fluorescent auramine-rhodamine stain. (microbenotes.com)
- The fluorochrome dye, auramine-rhodamine, forms a complex with mycolic acids found in the acid-fast cell wall of organisms that resist discoloration by acid-alcohol. (labtestsguide.com)
- Contains TB Auramine-Rhodamine reagent, double the amount of TB Decolorizer Fluorescent, and TB Permanganate reagent as counterstain. (chxa.com)
- Fluorochrome stains (eg, auramine-rhodamine) also identify acid-fast organisms, but a special fluorescent microscope is required. (msdmanuals.com)
Actin17
- Red: F-actin that has been stained with rhodamine-conjugated phalloidin. (molvis.org)
- Four general zones of f-actin movement are seen in primary cultures of newt lung epithelial cells microinjected with X-rhodamine actin. (ucsd.edu)
- Comparison of actin speckle microscopy with phalloidin staining. (ucsd.edu)
- Primary cultures of newt lung epithelial cells were microinjected with X-rhodamine actin. (ucsd.edu)
- Furthermore, actin staining is not appreciably different between species. (cytoskeleton.com)
- Actin staining is very useful in determining the structure and function of the cytoskeleton in living a nd fixed cells. (cytoskeleton.com)
- The actin cytoskeleton is a very dynamic and labile structure in the living cell, but it can be fixed with paraformaldehyde prior to probing or staining for actin structures. (cytoskeleton.com)
- Phalloidin has been labeled with a proprietary red fluorescent dye which allows it to be used to stain actin filaments in tissue cultured cells and tissue sections (1, see Figure 1) and cell-free preparations. (cytoskeleton.com)
- Acti-stain™ 555 phalloidin-labeled actin filaments retain many functional characteristics of unlabeled actin including their ability to interact with myosin. (cytoskeleton.com)
- Actin-stain™ 555 phalloidin is supplied as a red solid. (cytoskeleton.com)
- Actin Stress Fibers stained with Acti-stain™ 555 in a Swiss 3T3 cell. (cytoskeleton.com)
- Swiss 3T3 cell with activated Rho, nucleus is stained with Dapi (blue) and F-actin is stained with Acti-stain™ 555 (red F-actin, Cat. (cytoskeleton.com)
- Compare these performance characteristics to other fluorescent phalloidins and you will see the advantages of using Acti-stain™ for your actin staining requirements. (cytoskeleton.com)
- Neonatal bile duct explants treated with the toxin showed lumen obstruction with increased subepithelial staining for α-smooth muscle actin and collagen, consistent with fibrosis. (nih.gov)
- Rhodamine phalloidin is a high-affinity F-actin probe conjugated to the red-orange fluorescent dye, tetramethylrhodamine (TRITC). (thermofisher.com)
- Demonstrating very little nonspecific staining, Rhodamine phalloidin allows high-contrast discrimination of actin staining. (thermofisher.com)
- Rhodamine phalloidin can be used to visualize and quantitate F-actin in tissue sections, cell cultures, or cell-free preparations. (thermofisher.com)
Mycobacteria7
- Staining of the mycobacteria of tuberculosis with auramine 00 containing rhodamine S is quite specific. (dtic.mil)
- Stains other mycobacteria -- not specific for tuberculosis. (librepathology.org)
- Methods and RESULTS: Auramine-O-rhodamine (AR) staining and LIVE/DEAD BacLight Bacterial Viability staining (L/D staining) were adapted and evaluated for detection/quantification and differentiation (viable vs non-viable) of the MWF-associated mycobacteria and the background bacterial flora, respectively. (cdc.gov)
- The AR staining method was found to be specific to MWF mycobacteria with a minimum detection limit of 10 cells ml(-1) and was comparable to the QPCR in quantification efficiency in MWF matrix. (cdc.gov)
- The optimized AR staining- and the L/D staining-based microscopy methods have the potential for rapid, specific and differential assessment (viable vs non-viable) of MWF-associated mycobacteria and co-contaminants in field MWF. (cdc.gov)
- Stains commonly used to identify the mycobacteria are Ziehl-Neelsen and Fite. (medscape.com)
- Ziehl-Neelsen and Kinyoun stains are acid-fast stains used to identify mainly mycobacteria, particularly M. tuberculosis . (msdmanuals.com)
Counterstain4
- The counterstain Potassium Permanganate functions to stain the non-fluorescent tissues and the cell debris thus reducing possibilities of artifacts. (microbenotes.com)
- Eosin - a counterstain to haematoxylin, this stain colors red blood cells, cytoplasmic material, cell membranes, and extracellular structures pink or red. (microscopeworld.com)
- Malachite Green - a blue-green counterstain to safranin in Gimenez staining for bacteria. (microscopeworld.com)
- Safranin - a nuclear stain used as a counterstain or to color collagen yellow. (microscopeworld.com)
Tuberculosis5
- Mycobacterium tuberculosis or Nocardia) as the Ziehl-Neelsen stain, it is more affordable and more sensitive, therefore it is often utilized as a screening tool. (wikipedia.org)
- Ziehl-Neelson stain - used to look for Mycobacterium tuberculosis . (librepathology.org)
- Auramine stain is a fluorescent dye used in microbiology and histology to detect acid-fast bacteria, such as Mycobacterium tuberculosis. (labtestsguide.com)
- It is commonly employed in the Ziehl-Neelsen staining technique for tuberculosis diagnosis. (labtestsguide.com)
- This staining method allows for rapid and reliable identification of acid-fast organisms, aiding in the diagnosis and monitoring of tuberculosis and other related infections. (labtestsguide.com)
Bacteria2
- This is one of those techniques which was modified from the Acid Fast staining technique know as Ziehl-Neelsen Staining of Mycobacterium spp of bacteria. (microbenotes.com)
- Gram stain - for bacteria. (librepathology.org)
Fluorochrome dye1
- The fluorochrome dye, Auramine O, used in this stain reacts with the mycolic acids in the acid-fast cell wall of the organism and is refractory when rinsed with acid-alcohol (TB Fluorescent Decolorizer). (labtestsguide.com)
Cytoplasm1
- Phyloxin = pink -- stains muscle and cytoplasm. (librepathology.org)
Propidium Iodide1
- Cell death was assessed by Hoechst and propidium iodide staining. (diabetesjournals.org)
Ziehl-Neelsen2
- This technique was better than the Ziehl Neelsen Staining techniques. (microbenotes.com)
- If desired, the slide can be directly re-stained with one of the other acid-fast stains (Ziehl-Neelsen or Kinyoun Stain) after removing the immersion oil. (labtestsguide.com)
Nucleus2
- By using different stains, a nucleus or a cell wall are easier to view. (microscopeworld.com)
- Haematoxylin = blue -- stains nucleus. (librepathology.org)
Specimen2
- With the cover slip in place on top of the specimen, place a drop of stain on the edge of the cover slip. (microscopeworld.com)
- Wilson disease biopsy specimen with rhodanine stain (stain specific for copper deposition). (medscape.com)
Mycobacterium1
- Auramine and Rhodamine are both fluorescent dyes with a high affinity for mycolic acid found on the Mycobacterium spp cell wall Hence it stains the cell wall bright yellow or orange, under a fluorescent microscope with a green background. (microbenotes.com)
Dyes3
- A combination of Auramine and rhodamine produced satisfactory results as compared to the use of the dyes separately. (microbenotes.com)
- A decolorizing agent, acid alcohol is used to rinse off the none stained dyes. (microbenotes.com)
- Bucevičius J, Gerasimaitė R, Kiszka KA, Pradhan S, Kostiuk G, Koenen T, Lukinavičius G (2022) A general, highly efficient and facile synthesis of biocompatible rhodamine dyes and probes for live-cell multicolor nanoscopy. (uni-goettingen.de)
Assay1
- Rhodamine-123 accumulation assay was used to evaluate the pump function of P-gp. (greenmedinfo.com)
Truant1
- In 1962, Truant, Brett, and Thomas evaluated the usefulness of a fluorescent staining technique and found it to yield a larger number of positive smears than the conventional fuchsin-stained method. (labtestsguide.com)
Methylene Blue1
- Methylene Blue - stains animal cells to make nuclei more visible. (microscopeworld.com)
Gram3
- This stain is used in Gram Staining. (microscopeworld.com)
- Introduction Many bacterial cells stain easily using simple stains or a Gram stain. (chxa.com)
- Gram stain is the most common stain for general bacterial identification. (msdmanuals.com)
Reagents2
- The major difference between these two techniques is the kind of reagents used for staining and the mode of observing them. (microbenotes.com)
- Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator . (thermofisher.com)
Efflux1
- Spheroids treated with biliatresone had increased permeability as shown by rhodamine efflux within 5 hours compared with untreated spheroids, which retained rhodamine for longer than 12 hours. (nih.gov)
Ethidium Bromide1
- Ethidium Bromide - this stain colors unhealthy cells in the final stages of apoptosis, or deliberate cell death, fluorescent red-orange. (microscopeworld.com)
Acridine1
- Further, the nuclear and cellular damage was distinguished using Hoechst and Acridine orange/EtBr stain. (bvsalud.org)
Biological1
- Auramine phenol stain (AP stain) Biological stains Kommareddi S. (wikipedia.org)
Nuclear1
- Hematoxylin - a nuclear stain that, with a mordant, stains nuclei blue-violet or brown. (microscopeworld.com)
Nuclei2
- Neutral/Toluylene Red - stains nuclei red. (microscopeworld.com)
- Nile Blue - stains nuclei blue. (microscopeworld.com)
Confocal1
- Fibroblast contractility was assessed in three-dimensional collagen gel contraction assays, stress fiber assembly with rhodamine-phalloidin stains, and confocal microscopy. (nih.gov)
Immunofluorescence2
- Immunofluorescence and immunohistochemical staining were used to detect the expression of P-gp in DLBCL cells and tissues. (greenmedinfo.com)
- Tumor xenograft model and ocular pathological neovascularization model were constructed as well as Isolectin B4 (IsoB4) staining and immunofluorescence staining were used to assess the effects of AFAP1L1 on the progression of neoplasms and neovascular eye diseases in vivo. (biomedcentral.com)
Nocardia1
- Stains Nocardia . (librepathology.org)
Decolorizer1
- Flood the stain with Auramine Decolorizer. (labtestsguide.com)
Punctate2
- Control: Minimal α2-IR was detected in acinar cells (arrows), while strong α2-IR was observed in ductal cells as clustered punctate staining (arrowheads). (molvis.org)
- IAD: The distribution of α2-IR in LG from rabbits with IAD was very similar to that of control animals, i.e., α2-IR was mostly found in ductal cells as clusters of punctate staining. (molvis.org)
Mitochondria1
- Fuchsin - this stain is used to stain collagen, smooth muscle or mitochondria. (microscopeworld.com)
Microscopy4
- Osmium Tetroxide - used in optical microscopy to stain lipids black. (microscopeworld.com)
- Brachionus manjavacas stained with Lugol's iodine (a solution of potassium iodide with iodine in water) which interacts with polysaccharides, starches and glycogen in animals cells viewed by bright field microscopy. (canadiannaturephotographer.com)
- B. manjavacas stained with Toluidine blue and viewed by Bright field microscopy. (canadiannaturephotographer.com)
- The L/D staining-based microscopy allowed differential quantification of viable vs non-viable cells. (cdc.gov)
Hematoxylin1
- Hematoxylin and eosin stain of benign prostate gland . (librepathology.org)
Microscopic1
- One of the earliest methods devised for the detection of the tubercle bacillus is the microscopic staining technique. (labtestsguide.com)
Crystal Violet1
- Crystal Violet - stains cell walls purple when combined with mordant. (microscopeworld.com)
Collagen1
- Saffron = yellow -- stains collagen. (librepathology.org)
Smear4
- Rinse the stained smear with water until no color appears in the effluent. (microbenotes.com)
- Place the slide containing the fixed smear in a level staining rack. (labtestsguide.com)
- Let the smear stain for 15 minutes. (labtestsguide.com)
- Make sure the stain remains on the smear. (labtestsguide.com)
Interpretation1
- The evolution in staining methodologies has led to an era of modified staining techniques that are rapid, more versatile, and reliable in result interpretation. (microbenotes.com)
Membrane1
- The mitochondrial membrane potential was evaluated using the Rhodamine 123 stain. (bvsalud.org)
Commonly2
- Below is a list of commonly used stains, often for different types of cells. (microscopeworld.com)
- Rhodamine phalloidin is one of the most commonly used fluorescent phalloidin conjugates in the literature, as evidenced by over 1,500 citations (maintained in our in-house database). (thermofisher.com)
20201
- Bucevičius J, Kostiuk G, Gerasimaitė R, Gilat T, Lukinavičius G (2020) Enhancing the biocompatibility of rhodamine fluorescent probes by a neighbouring group effect. (uni-goettingen.de)
Hoechst2
- Hoechst Stains - two types of fluorescent stains, 33258 and 33342 are used to stain DNA in living cells. (microscopeworld.com)
- Bucevičius J, Keller-Findeisen J, Gilat T, Hell SW, Lukinavičius G (2019) Rhodamine-Hoechst positional isomers for highly efficient staining of heterochromatin. (uni-goettingen.de)
Proteins1
- Rhodamine phalloidin staining is fully compatible with other fluorescent stains used in cellular analyses including fluorescent proteins, Qdot nanocrystals, and Alexa Fluor conjugates including secondary antibodies. (thermofisher.com)
Rinse1
- A rinse of 800 ml methanol and 200 ml distilled water can be used to remove excess dye-stain from the item. (cbdiai.org)
Cells5
- Microscope cell staining is a technique used to enable better visualization of cells and cell parts under the microscope. (microscopeworld.com)
- Most stains can be used on non-living (fixed) cells, while only some types of stain can be used on living cells. (microscopeworld.com)
- Cells are primarily stained to enhance visualization of the cell or certain componenets. (microscopeworld.com)
- Cells are sometimes also stained to highlight metabolic processes or to differentiate between live and dead cells. (microscopeworld.com)
- In some situations you're blessed with an internal control , e.g. in renal tumours CD10 will stain RCC and the proximal tubule , in GISTs - CD117 the mast cells are positive. (librepathology.org)
Glycogen1
- Used to identify glycogen (together with PAS stain). (librepathology.org)
Blue2
- Nile Red / Nile Blue Oxazone - this stain is made by boiling Nile Blue with Sulfuric Acid, which creates a mix of Nile Red and Nile Blue. (microscopeworld.com)
- Staining is shown as a light brown color, haemotoxylin counter staining (blue). (elifesciences.org)
Detection1
- Wilkinson, D., and Misner, A., "A Comparison of Thenoyl Europium Chleate with Ardrox and Rhodamine 6G for the Fluorescent Detection of Cyanoacrylate Prints", Jor. (cbdiai.org)