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  • reagents
  • The reagents are used as probes, stains, markers, NIR-labels, pH-sensors, ion indicators, chelators and in other applications (e.g. analytical biochemistry, immunoassays and microscopy) by life science researchers in academia, biotechnology and the diagnostics & pharmaceutical industry. (bio-connect.com)
  • Cellular labeling with CellLight™ reagents employs BacMam technology, which uses a modified insect cell baculovirus coupled with a mammalian promoter as a vehicle to efficiently deliver and express genes in mammalian cells. (thermofisher.com)
  • The inability of baculoviruses to replicate in mammalian cells renders them safe as research reagents and provides a transient, footprint-free method to label cells. (thermofisher.com)
  • CellLight™ reagents provide a simple and effective method for introducing targeted intracellular labels into live cells, enabling unambiguous visualization by fluorescence microscopy of key organelles involved in membrane trafficking. (thermofisher.com)
  • tissue
  • Applications for slide assays include staining and imaging of tissue slices representing various diseased and normal states, performing cell-based assays in array format, and testing assays already developed on slides prior to moving to plate-based formats. (moleculardevices.com)
  • Herein, we present a new approach, virtual staining, that improves the Raman spectral histopathology (SHP) images of colorectal cancer tissue by combining the integrated Raman intensity image in the C-H stretching region (2800-3050 cm −1 ) with the pseudo-colour Raman image. (rsc.org)
  • The virtual staining image also represents the whole biochemical fingerprint, and several tissue components including carcinoma were identified automatically with high sensitivity and specificity. (rsc.org)
  • Mohs surgery is performed in four steps: Surgical removal of tissue (Surgical Oncology) Mapping the piece of tissue, freezing and cutting the tissue between 5 and 10 micrometers using a cryostat, and staining with hematoxylin and eosin (H&E) or other stains (Including Toluidine Blue) Interpretation of microscope slides (Pathology) Possible reconstruction of the surgical defect (Reconstructive Surgery) The procedure is usually performed in a physician's office under local anesthetic. (wikipedia.org)
  • After each surgical removal of tissue, the specimen is processed, cut on the cryostat and placed on slides, stained with H&E and then read by the Mohs surgeon/pathologist who examines the sections for cancerous cells. (wikipedia.org)
  • Gold particles can be created with a diameter of 1 nm (or lower) but another limitation is then realized-at these sizes the gold label becomes hard to distinguish from tissue structure. (wikipedia.org)
  • DAPI
  • Select a low magnification (1x-4x) and a single wavelength that can be used to view the entire sample with a short exposure, such as Transmitted Light (if available) or DAPI for a nuclear stain. (moleculardevices.com)
  • cell
  • Cell types previously not compatible with or poorly transduced with BacMam 1.0 can now be labeled with BacMam 2.0, including primary cells (e.g., neurons) and stem cells. (thermofisher.com)
  • Human dermal fibroblast cells were treated with these labeled polymers and rapid internalization and efficient cell staining was achieved within one hour, as judged by confocal microscopy. (techconnectworld.com)
  • This staining method is useful to localize the cell body, as it can be seen in the soma and dendrites of neurons, though not in the axon or axon hillock. (wikipedia.org)
  • Size
  • For more information and guidelines for selecting a kit size, please download the Mix-n-Stain™ flyer and the product information sheet for this product. (biotium.com)
  • Methods
  • Pathways through cerebral white matter can be charted by histological dissection and staining, by degeneration methods, and by axonal tracing. (wikipedia.org)
  • specific
  • View our specific categories below to easily locate the right Stain, Dye, Probe or Label for your research needs. (scbt.com)
  • images
  • The morphology of the virtually stained images is in agreement with the gold standard in medical diagnosis, the haematoxylin-eosin staining. (rsc.org)
  • interest
  • The electron-dense gold particle can now be seen under an electron microscope as a black dot, indirectly labeling the molecule of interest. (wikipedia.org)
  • sheet
  • If one imagines an aluminum pie pan as the blood covered surgical margin, and the top of the pie is the crust covered surface of the skin - the goal is to flatten the aluminum pie pan into one flat sheet, mark it, stain it, and examine it under the microscope. (wikipedia.org)
  • record
  • In 2010, he created "Stained Music", his own record label. (wikipedia.org)
  • The first track they produced "I Don't Want It To End" was picked up by the record label EMI / Universal Music Group and reached number 1 in the iTunes chart. (wikipedia.org)
  • emission
  • The filters and the dichroic beamsplitter are chosen to match the spectral excitation and emission characteristics of the fluorophore used to label the specimen. (wikipedia.org)