Stained smear. Medical search. Frequent questions

Giemsa staining of conjunctival scrapings may aid in characterizing the inflammatory response. (medscape.com)
The different types of WBC and their percentages can be determined by doing a differential count on a blood smear, which has been stained with Wright's or Giemsa stain. (uwyo.edu)
This dataset contains 186 digital images of May Grünwald-Giemsa (MGG)-stained peripheral blood (PB) smears. (mendeley.com)
in a thin blood smear stained with Giemsa, showing pleomorphic rings and tetrad forms. (norvect.no)
It can be used if rapid results are needed, but should be followed up when possible with a confirmatory Giemsa stain, so that Schüffner's dots can be demonstrated. (cdc.gov)
Kept tightly stoppered and free of moisture, stock Giemsa stain is stable at room temperature indefinitely (stock stain improves with age). (cdc.gov)
Pipet from this tube to prepare the working Giemsa stain. (cdc.gov)
Prepare fresh working Giemsa stain in a staining jar, according to the directions above. (cdc.gov)
Pour 40 ml of working Giemsa buffer into a second staining jar. (cdc.gov)
Place slides into the working Giemsa stain (2.5%) for 45-60 minutes. (cdc.gov)
Remove thin smear slides and rinse by dipping 3-4 times in the Giemsa buffer. (cdc.gov)
As alternates to this 45-60 minutes in 2.5% Giemsa stain, the smears could be stained for shorter times in more concentrated stains. (cdc.gov)
To ensure that proper staining results have been achieved, a positive smear (malaria) should be included with each new batch of working Giemsa stain. (cdc.gov)
Slides stained with Giemsa can be used, but it is easiest to find the parasite by microscopic examination of fresh wet preparations, because the trypanosomes are motile. (cdc.gov)
in a thin blood smear stained with Giemsa. (cdc.gov)
For best results, the smear should be stained with a 3% Giemsa solution (pH of 7.2) for 30-45 minutes. (cdc.gov)
Babesiosis is usually diagnosed by microscopic examination of Giemsa-stained or Wright-stained thin or thick blood smears. (medscape.com)
Wright-stained or Giemsa-stained peripheral blood smears reveal intraerythrocytic ring forms with a central pallor. (medscape.com)

Because organisms are intracellular, attempt to obtain specimens in a manner that will contain mucosal cells and not merely discharge (similar to a Papanicolaou smear). (medscape.com)
A Papanicolaou (Pap) smear test was performed. (cytojournal.com)
Screening tests for cervical cancer include the Papanicolaou test (Pap smear) and the HPV DNA test. (webmd.com)

A Novel and Rapid Smear Cytomorphology Detection Strategy Based on Upconversion Nanoparticles Immunolabeling Integrated with Wright's Staining for Accurate Diagnosis of Leukemia. (bvsalud.org)
To achieve simultaneous determination , NaYF4Yb,Er UCNPs were coupled with CD38 antibodies to construct immunofluorescence probes that were developed to bind to diffuse large B cell lymphoma (DLBCL) cells , followed by Wright's staining that has been widely used in clinical work for morphological diagnosis . (bvsalud.org)
Wright's stain, 1,000X). (merckvetmanual.com)

Sputum stain for mycobacteria is a test to check for a type of bacteria that cause tuberculosis and other infections. (medlineplus.gov)
Failure to follow these directions may cause staining artifacts and disrupt the normal morphology of bacteria and cells. (sciencecompany.com)
A sputum Gram stain is a laboratory test used to detect bacteria in a sputum sample. (mountsinai.org)
The Gram stain method is one of the most commonly used methods to rapidly identify the type of bacteria in a bacterial infection, including pneumonia. (mountsinai.org)
The lab team member looks at the stained slide under a microscope, checking for bacteria and white blood cells. (mountsinai.org)
These bacteria were often absent from gram stain smears of patients with other bacterial morphotypes. (cdc.gov)
Gram staining technique is used for staining bacteria, yeasts and aerobic actinomycetes. (who.int)
Gram stain is the most common stain used in microbiology to identify bacteria. (medscape.com)
Organisms without a cell wall (eg, Mycoplasma species) and small bacteria (eg, Chlamydia and Rickettsia species) do not stain with Gram stain. (medscape.com)

Blood smear with different kin. (inds.co.uk)
Used in hematology, this stain is not optimal for blood parasites. (cdc.gov)
Make as many thin smears as possible, preferably within one hour after the blood was drawn from the patient. (cdc.gov)
This Stain Kit is used for the detection of ferric iron in tissues, blood smears, or bone marrow smears and is based on the Prussian Blue reaction in which ionic iron reacts with acid ferrocyanide producing a blue color. (gentaur.fr)
An immunoalkaline phosphatase method was developed using the OCI-AML3 cell line, known to have mutated NPM1, and assessed on blood and marrow smears of 60 AML cases. (ox.ac.uk)
Thick blood smears are more sensitive in detecting malaria parasites because the blood is concentrated, allowing a greater volume of blood to be examined. (cdc.gov)
Common errors in reading malaria smears are caused by platelets overlying a red blood cell, concerin about missing a positive slide, and misreading artifacts as parasites. (cdc.gov)
Persons suspected of having malaria but whose blood smears do not demonstrate the presence of parasites should have blood smears repeated approximately every 12-24 hours for 3 consecutive days. (cdc.gov)
Blood smear of cat. (flinnsci.com)
A systematic search in four databases (PubMed, Scopus, Web of Science, and ScienceDirect) and Google Scholar was performed via a search strategy using Machine Learning (ML), leukemia, peripheral blood smear (PBS) image, detection, diagnosis, and classification as the keywords. (hindawi.com)
These studies have analyzed blood smears images for diagnosing, differentiating, and counting the cells in various types of leukemia [ 7 , 8 ]. (hindawi.com)
The rugs were smeared with blood and urine stains. (radaronline.com)
Photomicrograph of a blood smear from a dog with a reactive lymphocyte, a nonspecific indicator of immune stimulation. (merckvetmanual.com)
[ 4 , 6 ] Guidelines from Infectious Diseases Society of America (IDSA) recommend confirmatory testing for babesiosis with a blood smear or PCR. (medscape.com)
Blood smear showing Babesia species in erythrocytes. (medscape.com)
While in general we have four vital signs, in hematology, examining the peripheral blood smear is the fifth one. (hematology.org)
Similar to the role of other vital signs, a normal peripheral blood smear is as helpful as an abnormal one in the journey to establishing a diagnosis. (hematology.org)
Prior to starting a hematology and oncology fellowship, I did not know how to look at a blood smear, where or how to get one, or what to do with it. (hematology.org)
I remember pictures of blood smears along with buzz words of different cell names used to indicate specific diseases commonly tested in exams. (hematology.org)
In fellowship, I learned that a blood smear can assist my argument toward a correct diagnosis and can guide further workup and management. (hematology.org)
Blood smears are usually done by lab technicians. (hematology.org)
This will help to assess the overall quality of the smear, abnormal red blood cell (RBC) distribution (e.g., rouleaux), and/or rapid detection of large abnormal cells (e.g., blasts). (hematology.org)
The best way to build and improve blood smear reading skills is practice. (hematology.org)
Being skilled in reviewing blood smears is rewarding indeed! (hematology.org)
Always remember to check the fifth vital sign in hematology: the patient's blood smear. (hematology.org)
Retrieved from https://www.hematology.org/education/trainees/fellows/trainee-news/2021/blood-smear . (hematology.org)
https://www.hematology.org/education/trainees/fellows/trainee-news/2021/blood-smear (label-accessed December 07, 2023). (hematology.org)
I found toenail clippings in the sink, bottom of bathtub looked like something died there, pubic hairs in the bath towels, hairballs in the bed, blood stains in the sheets, heater spit out dust everywhere. (consumeraffairs.com)
This Wright-stained blood smear photomicrograph depicted a developing, Plasmodium malariae schizont. (cdc.gov)
Gram stains can be made from blood culture and solid culture. (who.int)

Organisms that retain the violet-iodine complexes after washing in ethanol stain purple and are termed Gram-positive, those that lose this complex stain red from the safranin counterstain and are termed Gram-negative. (sciencecompany.com)

Figure 1 shows the stained slides of the most common different types of leukemia. (hindawi.com)
Tray for staining slides. (medscape.com)

For every case, skin tissue was punched out to get biopsy for HP and FF staining and incised for SSS. (njlm.net)
Investigations include smears, biopsy, staining with periodic acid-Schiff (PAS) or Gomori methenamine silver, culture of the affected tissues, polymerase chain reaction (PCR), serodiagnosis (sometimes), physical examination, and chest radiography. (medscape.com)

The cervical smear findings showed both cellular components which raised the suspicion of carcinosarcoma. (cytojournal.com)

Specimens should be obtained for culture and smear if inflammation is severe, in chronic or recurrent infections, with atypical conjunctival reactions, and with failure to respond to treatment. (medscape.com)

In the algorithms, examination of Gram-stained genital smears in female sex workers without clinical signs of cervicitis improved sensitivity without altering specificity for the diagnosis of cervical infection. (nih.gov)
The PAP smear is the most popular test used for diagnosis of cervical epithelial lesions, however, due to differential diagnostic difficulties in cytologic al smears , methods are searched for what would be more reliable and would enable exact evaluation of each case. (iium.edu.my)
If smears remain negative, then the diagnosis of malaria is unlikely. (cdc.gov)
Using the ML method to process leukemia smear images can improve accuracy, reduce diagnosis time, and provide faster, cheaper, and safer diagnostic services. (hindawi.com)
Diagnosis is by demonstrating parasites in smears or cultures and increasingly by polymerase chain reaction (PCR)-based assays at reference centers. (msdmanuals.com)

Thick smears should be left in buffer for 5 minutes. (cdc.gov)
the thick smear is stained unfixed. (cdc.gov)
However, thick smears are more difficult to read, and thin smears may be preferred by laboratories that have limited experience. (cdc.gov)
As an alternative to a damp cloth, smearing a thick film of dish detergent on the glue spot, and allowing that to stand for a few hours, will often soften the glue sufficiently for it to be scraped away. (ehow.com)
The specimen is spread on a slide and a thin smear should be prepared, as thick smears may diminish the accuracy of interpretation. (medscape.com)
thin smears require shorter decolorizing time than thick smears. (medscape.com)

Preparation of the smear. (sciencecompany.com)
The first consideration is the correct preparation of the smear. (sciencecompany.com)
It is important to have personnel who are experienced in preparation and review of these smears. (medscape.com)

These organisms were usually filamentous, gram positive rods of varying length that often form chains, but occasionally, they stained gram negative. (cdc.gov)
They are often thin, wispy, eyelash-like faintly staining curved gram negative rods. (cdc.gov)
Gram-negative organisms take the counterstain and stain pink/red. (medscape.com)

The Gram stain procedure was originally developed by the Danish physician Hans Christian Gram to differentiate pneumococci from Klebsiella pneumonia. (sciencecompany.com)
In brief, the procedure involves the application of a solution of iodine (potassium iodide) to cells previously stained with crystal violet or gentian violet. (sciencecompany.com)
Staining procedure. (sciencecompany.com)
If still unacceptable, check the quality of the reagent, check if the staining procedure was followed correctly and check if the correct reference strains were used. (who.int)

however, Wright stain alone will not reliably stain Plasmodium parasites. (cdc.gov)
Plasmodium parasites are always intracellular, and they demonstrate, if stained correctly, blue cytoplasm with a red chromatin dot. (cdc.gov)

b) Cytological cervical smears examination showed tissue fragments of round to oval hyperchromatic nuclei with scant cytoplasm in necrotic debris background (Pap, ×10). (cytojournal.com)

In a study from Sudan, oral scrape smear cytological analysis has been proposed as a useful early diagnostic method for epithelial atypia and therefore also for malignant oral lesions 1 . (bvsalud.org)

Those organisms that retain the crystal violet dye will stain blue, those that do not are stained red with safranin. (who.int)

Giant chromosomes, smear from salivary gland of Chironomus, carefully fixed and stained prepared microscope slide. (inds.co.uk)
After the slide was gram stained the slide was scanned under a microscope using low power objective to locate clusters of epithelial cells. (cdc.gov)
The smear was examined with a light microscope. (europa.eu)
After staining, the slide is examined under microscope with oil immersion. (medscape.com)

It's extremely darkly colored and known for leaving tough stains. (yahoo.com)
Learn how to remove tough stains from fiberglass tubs with ease. (lovetoknow.com)
If you have tough stains on the textured bottom of your tub, don't despair. (lovetoknow.com)

Gram stains, cultures, and nucleic acid amplification tests (NAATs) of genital, rectal, conjunctival, pharyngeal secretions and/or other extragenital sites, as applicable, should also be obtained when DGI is suspected. (medscape.com)

This sputum stain test can give your doctor a quick answer. (medlineplus.gov)

Stained smears from patients with Babesia infection, in addition to having these intraerythrocytic ring forms, may also demonstrate merozoites arranged in a tetrad configuration resembling a Maltese cross. (medscape.com)
however, Babesia may be easier to detect on thin smears due to the size of the organisms. (medscape.com)

In addition, the 2000mm tall wet room shower screen prevents shower spray into the bathroom, and the 10mm glass thickness provides extra strength and is very easy to clean while resisting smears and stains. (betterbathrooms.com)

Direct immunofluorescence monoclonal antibody staining and enzyme-linked immunosorbent assay (ELISA) are rapid and widely available detection techniques. (medscape.com)
To evaluate the efficacy of currently available laboratory tests like M.leprae-specific repetitive element Polymerase Chain Reaction (RLEP-PCR) and conventional methods like, Slit Skin Smears (SSS), Histopathology (HP) and Fite-Faraco (FF) staining for detection of M. leprae in clinically diagnosed cases of early-stage leprosy. (njlm.net)
Improvement in case detection of 39%, 29.3%, and 12.2% by PCR over SSS, FF Stain and HP respectively proved superiority of PCR over other procedures. (njlm.net)

The cells that are previously stained with crystal violet and iodine are next treated with a decolonizing agent such as an ethanol/acetone mixture. (sciencecompany.com)
Your doctor may use it if a Pap smear finds unusual cells. (webmd.com)
They stain your cervix with a harmless dye or acetic acid so the cells are easier to see. (webmd.com)
When cells are stained with crystal violet, crystal violet binds to the cell wall. (medscape.com)

In individuals with asymptomatic infection, smear results may be negative. (medscape.com)

A variation of Gram staining is called Atkin Gram stain, in which the primary stain is gentian violet and mordant is Atkin iodine. (medscape.com)

Can cytoplasmic nucleophosmin be detected by immunocytochemical staining of cell smears in acute myeloid leukemia? (ox.ac.uk)

After staining, representative areas in each slide were selected and NORs were counted. (iium.edu.my)
One SSS slide was stained by modified Ziehl-Neelsen (ZN) staining to demonstrate Acid Fast Bacilli (AFB). (njlm.net)
If the slide is correctly stained, some types of leukemia can be more easily identified and distinguished than others, but more equipment is needed to determine underlying leukemia. (hindawi.com)
Approximately two-thirds to three-fourths of the slide length should be covered by the smear with no irregularities, streaks, or holes. (hematology.org)

These make stain less susceptible to decolorization. (medscape.com)

The latter will prove useful if a problem occurs during the staining and/or if you wish later to send the smears to a reference laboratory. (cdc.gov)

You can try rubbing baking soda into stains that aren't coming out, too. (yahoo.com)
If any stain remains, sprinkle baking soda on a wet toothbrush. (lovetoknow.com)
To get rid of mold or bathmat stains, you need a bit of baking soda and vinegar. (lovetoknow.com)

At the end of each clinic, we sat down and went over all the patients' smears, the normal ones and the abnormal ones, which made me realize the importance of examining numerous normal smears in order to accurately identify abnormalities. (hematology.org)
The DNA test may be done at the same time as the Pap smear or afterward, in case of abnormal Pap test results. (webmd.com)

Allow the smears to dry quickly, using a fan or blower at room temperature. (cdc.gov)
Stains are stored at room temperature. (who.int)

Air dry the thin film, fix it with methyl alcohol, and immediately stain it. (cdc.gov)
The smear should be thin and not clumped. (medscape.com)

From there, blot out the stain with dish detergent or stain remover. (yahoo.com)

the shorter stains yield faster results, but use more stain and might be of less predictable quality. (cdc.gov)

To save your carpet, upholstery, and linens you'll need a stain remover from the grocery store, ice, and detergent. (yahoo.com)
Avoid the stress of ruining your clothes or dropping your pie this holiday season by stashing stain remover in your purse. (yahoo.com)
Better Homes and Gardens suggests Shout stain remover for chocolate stains. (yahoo.com)
With clothing, it's as simple as rubbing the stain remover into the fabric and rinsing it with water until the stain lifts. (yahoo.com)

This usually results in the best staining which will be needed for proper interpretation. (hematology.org)
Record the QC results on the Gram stain QC sheet provided in Annex 1. (who.int)

Looking at a smear for a different patient may result in misdiagnosis and subsequent inappropriate testing. (hematology.org)

Filter a small amount of this stock stain through Whatman #1 filter paper into a test tube. (cdc.gov)

The appearance of organisms that stain gram-positive or -negative means that the smear contains organisms. (medscape.com)

When you're ready to apply your preferred stain, make a "V" at the bow and outside corners of your lips and a "U" towards the bottom of your lips, according to beauty expert Andrea Claire. (piecestopeaces.com)
To ensure the longest wear, make sure to apply your stain on clean, dry lips," Garner advises Allure. (piecestopeaces.com)
Use cold water because hot water will make the stain more permanent. (yahoo.com)

Allow yourself to be spoiled with Yves Saint Laurent's Vinyl Cream Lip Stain, a high-shine hue that will put the glossiest lip glosses in your makeup bag to shame. (piecestopeaces.com)

Use a clean, white cloth, to avoid the possibility of dye staining your leather. (ehow.com)

This means that counts of Ag-NOR could be of great diagnostic value for the cytopathologists in conjunction with routine pap stain to evaluate cellular proliferative activity of the lesion and for follow up of patients after treatment to detect regression in the severity of the lesion. (iium.edu.my)

This Gram Stain Set consists of four chemicals, each in a handy four ounce HDPE flip top dropper bottle. (sciencecompany.com)

Anatomy12

Organisms19

Diseases51

Chemicals and Drugs28

Analytical, Diagnostic and Therapeutic Techniques and Equipment65

Psychiatry and Psychology2

Phenomena and Processes8

Disciplines and Occupations6

Technology, Industry, Agriculture5

Humanities1

Information Science3

Health Care27

Geographicals12

Giemsa18

Papanicolaou3

Wright's3

Bacteria9

Blood30

Termed Gram-positive1

Slides2

Biopsy2

Cervical smear1

Specimens1

Diagnosis5

Thick6

Preparation3

Gram-negative3

Procedure4

Parasites2

Cytoplasm1

Cytological1

Safranin1

Microscope4

Tough stains3

Genital1

Sputum1

Babesia2

Thickness1

Detection3

Cells4

Infection1

Gentian1

Acute1

Slide4

Decolorization1

Laboratory1

Baking soda3

Abnormal2

Room temperature2

Thin2

Dish1

Shorter1

Remover4

Results2

Subsequent1

Test1

Organisms1

Make3

Shame1

Cloth1

Detect1

Consists1