Two-cell stage albino embryos were injected unilaterally into the animal hemisphere of one blastomere with 10-20 ng of XMeis3 MO. (xenbase.org)
A) Two-cell stage albino embryos were injected unilaterally into the animal hemisphere of one blastomere with 100 pg of RNA encoding the HoxD1 antimorph protein. (xenbase.org)
Xenopus3
This video guide is an essential learning tool for investigators considering work with Xenopus eggs and embryos. (cshlpress.com)
The embryo of the frog Xenopus laevis is widely used as a model of cell polarization, migration, and morphogenesis due to its unique experimental advantages. (nature.com)
In Xenopus embryos, XMeis3 protein activity is required for normal hindbrain formation. (xenbase.org)
Axial3
Simultaneously, on the dorsal side of the embryo, axial and paraxial mesoderm tissues undergo convergent extension which elongates the anterior-posterior axis and aids blastopore closure. (nature.com)
We analyzed the morphological features of dying cells in the developing axial structures of 5 human embryos between 5 and 8 weeks of postovulatory age. (karger.com)
According to our findings, apoptosis seems to be the most frequently observed type of PCD, but it is not the exclusive type of morphological cell death during the development of axial structures in human embryos. (karger.com)
Vitro3
Identification of the relative embryonic autonomy as a critical stage in embryo culture in vitro, along with morphological, histological, and physiological statuses of the relatively autonomous embryos, is considered. (springer.com)
These findings were confirmed in later studies in which removal of mesenchyme from embryonic day 12 murine thymic lobes impaired thymus growth in vitro ( 8 ). (frontiersin.org)
We characterize the activation kinetics of these reagents in vitro and demonstrate their efficacy in zebrafish embryos that express NfsB either ubiquitously or in defined cell populations. (cdc.gov)
Drosophila1
In the placodes of the forming tubes of the salivary glands in the Drosophila embryo, we previously identified spatially defined cell behaviors of cell wedging, tilting, and cell intercalation that are key to the initial stages of tube formation. (elifesciences.org)
Embryonic period2
The embryonic period (first 8 wk after conception) is divided into 23 stages according to the Carnegie system. (medscape.com)
During stage 4 (day 20 of embryonic period), the foregut begins its appearance and the respiratory diverticulum, from which the respiratory system develops, is identified in a medial position. (medscape.com)
Dorsal3
Like the original allele, a fraction of mutant embryos die with cuticular defects, notably head involution and dorsal closure defects. (peerj.com)
A fraction of these mutant embryos have lethal head involution or dorsal open phenotypes, and have an extended phenocritical period. (peerj.com)
the red arrows mark the dorsal midline in A-D. (A) N-tubulin expression is inhibited in 91% of the embryos (n = 100/110). (xenbase.org)
Embryogenesis2
This is one of the critical stages of embryogenesis, when an immature embryo becomes independent of some physiological factors, in particular, hormones (mainly auxins, cytokinins, and ABA) of a maternal organism. (springer.com)
Embryonic defects are much more extreme in germline clones, where the majority of mutant embryos die during embryogenesis and do not form cuticle, implying a strong chem maternal contribution. (peerj.com)
Morphology1
Embryo morphology al ows options, the discovery of cell-free DNA in the evaluation of its growth, viability, and biological fluids has led to major advances in implantation capacity. (who.int)
Gene7
This study, published in Nature Biotechnology , combines scRNAseq data with spatially-resolved expression profiles to generate an atlas of gene expression at single-cell resolution across the entire embryo. (embl.org)
According to AceView, this gene is expressed at high level , 2.6 times the average gene in this release, at all stages of development [Kohara cDNAs]. (nih.gov)
The sequence of this gene is defined by 16 cDNA clones and 18 elements defined by RNA-seq, some from mixed (seen 7 times), embryo (5), l2 (once). (nih.gov)
HoxD1 is an XMeis3 direct-target gene, and ectopic HoxD1 expression rescues cell fate losses in either XMeis3 or Zic protein knock down embryos. (xenbase.org)
Expression of the proneural gene (not shown), Xngnr1 is inhibited in 77% of the embryos (n = 48/62). (xenbase.org)
We and others have synthesized light-activatable cMOs that allow spatiotemporal control of RNA splicing or translation, complementing the use of conditional knockouts to study stage- and tissue-specific differences in gene function. (cdc.gov)
11 , 12 Light-dependent gene silencing can be achieved through whole-organism irradiation or the targeted illumination of specific cell populations. (cdc.gov)
Genetic1
Because one egg was fertilized by one sperm, the genetic material in the two embryos is the same. (msdmanuals.com)
Target genes2
It's also work we can build upon to include more target genes across different stages of development. (embl.org)
FGF3 and FGF8 are direct target genes of XMeis3 protein and their expression is lost in XMeis3 morphant embryos. (xenbase.org)
Cells12
This is what distinguishes a sack of cells from an embryo. (strata-sphere.com)
Unlike the global warming mythology and the disgusting harvesting of embryos for magical stem cells, the science of creation is not in doubt. (strata-sphere.com)
In order to break the initial "egg shape" of the embryo, cells need to polarize in a precise and coordinated manner. (nature.com)
Cells organised according to their transcript data changing to the seqFISH mouse embryo map. (embl.org)
Studies using single-cell RNA sequencing (scRNAseq) have provided insights into how the molecular landscape of cells in the mouse embryo changes during early development. (embl.org)
However, scRNAseq methods require the cells in the embryo to be dissociated - or separated - which means spatial information is lost. (embl.org)
This stage of development was of particular interest because it is when the cells within the embryo start to differentiate, or become a specific cell type. (embl.org)
In this period a fertilized egg will be created, a group of cells will be in charge in different process, first the blastocyst which is a group a cell that will develop into the embryo. (bartleby.com)
In those men in whom spermatogenesis is blocked at the stage of round spermatids, in which meiosis has already been completed, these round cells can successfully fertilize oocytes after being injected into their cytoplasm. (wikipedia.org)
The inner cells in the thickened area develop into the embryo, and the outer cells burrow into the wall of the uterus and develop into the placenta. (msdmanuals.com)
In vivo and in organized cells, and proper symmetry are healthy individuals, macrophages can characteristics of higher-quality embryos, which phagocytize DNA that has been passively point to healthy development and higher rates of released into the blood from apoptotic or necrotic implantation. (who.int)
Low-quality embryos, on the other cells, thereby maintaining a relatively low basal hand, frequently display morphological level [16-18]. (who.int)
Tissues1
Autonomy is a special structural and functional state of an embryo, reflecting its ability for self-regulation and independence from the adjacent tissues. (springer.com)
Experiments2
The interested reader may find the extremes in the data supplied from the most interesting embryo transfer experiments by Allen et al. (ivis.org)
In the described experiments, Krox20 expression is inhibited in 94% of the embryos (n = 190/202). (xenbase.org)
Sperm2
In cases of nonobstructive (secretory) azoospermia, on the other hand, testicular sperm production is blocked at different stages of the process of sperm formation (spermatogenesis). (wikipedia.org)
After intracytoplasmic sperm injection (ICSI), 48 embryos were evaluated on day 3 of their development, according to their cell number. (who.int)
Develop3
If all requirements for round spermatid selection and injection are successfully met, the injected oocytes develop to early embryos and can be transferred to the mother's uterus to produce pregnancy. (wikipedia.org)
Embryos with strongly disrupted apico-basal polarity do not develop, and result in lethal mutant phenotypes where only small pieces of cuticle are synthesized. (peerj.com)
During this important stage of human development, the respiratory and digestive tracts develop separately. (medscape.com)
Study4
The researchers carried out this study using 8-12 somite stage mouse embryos. (embl.org)
According to AIMS scientist, Dr Andrew Negri the novelty of the study is that it explored the effects of insecticides on many different life stages of the coral. (aims.gov.au)
In addition, the study found that coral at all life stages was particularly sensitive to the agricultural fungicide MEMC, which caused bleaching in adult corals at levels so low as to be scarcely measurable. (aims.gov.au)
This prospective study was conducted with 96 spent CM from patients undergoing IVF cycle, in order to determine relationships of Cell-free DNA levels in embryo CM with embryo cleavage quality on day 3. (who.int)
Human2
This historic 1957 paper by O'Rahilly is a description of the development of the human embryo limb cartilage. (edu.au)
Streeter (1929) considered using the order of chondrification established by Senior as an aid in staging human embryos. (edu.au)
Development6
Senior pointed out that "chondrification, being cumulative, affords an excellent guide to the relative stage of development of embryonic limbs while the increase in the number of centers lasts. (edu.au)
They combined this information with scRNAseq data to produce an atlas of cell types found across this stage of mouse development. (embl.org)
Once fertilized, the egg is transformed into a pre-embryo and it has to be transferred to the uterus to continue its development. (wikipedia.org)
A baby goes through several stages of development, beginning as a fertilized egg. (msdmanuals.com)
[ 1 ] There is no evidence of identifiable respiratory system development during the first 8 stages of the Carnegie system. (medscape.com)
Tracheoesophageal septum at stage 12 embryonic development, separating the foregut into the esophagus and laryngotracheal tube. (medscape.com)
Critical1
Dr Andrew Baird of the ARC CoE for Reef Studies says "These developmental stages and events are critical points in the life history of corals. (aims.gov.au)
Period2
According to O'Rahilly and Tucker, this stage marks the period when the "respiratory tap" is turned on. (medscape.com)
At the beginning of the fetal period (30 mm crown-rump [C-R] stage), the chondrocytes are well identified within the incomplete rings of the trachea. (medscape.com)
Length1
When juvenile masu salmon were subjected to feeding manipulations during the first spring/summer, only fish exceeding a fork length of 12 cm exhibited an increased gill NKA activity. (bvsalud.org)
Life6
Too many people think an 'embryo' is a form of life. (strata-sphere.com)
The term refers to a stage of life shared by numerous animals (including those who lay eggs to give birth). (strata-sphere.com)
In addition, the high sensitivity of coral settlement also suggests current water quality guidelines may not adequately protect all coral life stages. (aims.gov.au)
Markey KL, Baird AH, Humphrey C, Negri A (2007) Insecticides and a fungicide affect multiple coral life stages. (aims.gov.au)
The respiratory diverticulum then migrates caudally into the mesenchyme ventral to the foregut at 26 days of embryonic life (stage 12) (see the image below). (medscape.com)
Section1
This new approach means that we can take a section of the embryo and essentially paint on the cell types using different colours to place cell type context on the anatomy of the embryo at single-cell resolution. (embl.org)
Found2
In a 27-mm. embryo, Schulin (1879) found that all the skeletal elements of the hand were ehondrified except the sesamoids. (edu.au)
Leboucq (1886) stated that he found the tarsal elements differentiated in a 12-mm. embryo, but it is not clear from his account whether he intended to use "differentiated" as synonymous with "chondrified. (edu.au)
Quality2
Understanding the association between Cell-free DNA levels in embryo CM and the quality of embryo cleavage could help improve the quality of IVF techniques. (who.int)
Due to its clinical applications success of IVF results is the morphological and the expansion of non-invasive treatment quality of the embryo. (who.int)
Normal8
E) Zic1 expression is normal in 96% of the embryos (n = 26/27). (xenbase.org)
F) Zic2 expression is normal in 96% of the embryos (n = 23/24). (xenbase.org)
G) Zic5 expression is normal in 91% of the embryos (n = 91/100). (xenbase.org)
H) Sox2 expression is normal in 95% of the embryos (n = 20/21). (xenbase.org)
J) Pax3 expression is normal in 85% of the embryos (n = 28/33). (xenbase.org)
K) c- Myc expression is normal in 83% of the embryos (n = 25/30). (xenbase.org)
L) Id3 expression is normal in 84% of the embryos (n = 32/38). (xenbase.org)
e) Pax3 expression is normal in 100% of the embryos (n = 16/16). (xenbase.org)
Expression11
Compared to controls, in chem mutants in embryonic lateral epithelia Crumbs expression is mislocalized and reduced, Coracle is increased and mislocalized basally at embryonic stages 13-14, then reduced at stage 16. (peerj.com)
B) XMyt1 expression is inhibited in 100% of the embryos (n = 12/12). (xenbase.org)
C) FoxD3 expression is inhibited in 83% of the embryos (n = 116/139). (xenbase.org)
D) Slug expression is inhibited in 75% of the embryos (n = 70/93). (xenbase.org)
15% of the embryos had minor expression reductions. (xenbase.org)
17% of the embryos had minor expression reductions. (xenbase.org)
16% of the embryos had minor expression reductions. (xenbase.org)
a) Krox20 expression is inhibited in 87% of the embryos (n = 83/95). (xenbase.org)
b) N-tubulin expression is inhibited in 97% of the embryos (n = 57/59). (xenbase.org)
c) Slug expression is inhibited in 85% of the embryos (n = 44/52). (xenbase.org)
d) FoxD3 expression is inhibited in 87% of the embryos (n = 52/60). (xenbase.org)
Order1
This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal uterus. (wikipedia.org)
Growth1
With the continuous growth of the respiratory diverticulum, at about 22 days (stage 10), the primitive pharynx is visible, among the branchial arches and pouches. (medscape.com)
Specific1
Each specific stage is defined by specific morphologic criteria. (medscape.com)
Levels4
Day 2 and day 3 CM corresponding to each one of the embryos was analyzed, by quantitative PCR, for estimation of Cell-free DNA levels. (who.int)
The results revealed a significant increase in Cell-free DNA levels on day 2 CM corresponding to 4 to 6 cell embryos compared to those corresponding to 7 to 8 cel embryos (p=0.04). (who.int)
As for day 3 CM, the results showed no significant difference between the Cell-Free DNA levels in CM of 7-8 and those of 4-6 cell embryos (p=0.4). (who.int)
We conclude that cel -free DNA levels in CM might be associated with delayed embryo cleavage. (who.int)