Stable incorporations of T-DNA into the citrus genome have been confirmed with both histochemical staining of GUS activity and molecular analyses. (springer.com)
However, they are completely different in their purposes, as gene therapy aims to cure a defect in cells, and transgenesis seeks to produce a genetically modified organism by incorporating the specific transgene into every cell and changing the genome. (wikipedia.org)
Typical plant transformation protocols to produce transgenic, genetically modified (GM) varieties rely on transgenes, chemical selection, and tissue culture. (frontiersin.org)
Lentiviral2
Here we demonstrate for the first time a cure of HT1 by direct, in vivo administration of a therapeutic lentiviral vector targeting the expression of a human fumarylacetoacetate hydrolase ( FAH ) transgene in the porcine model of HT1. (biorxiv.org)
Lentiviral vectors deliver transgenes into transcriptionally active regions of the target chromatin, also favouring stable protein expression. (gtp-bioways.com)
Expression17
Some examples include delivering DNA-free gene editing reagents such as ribonucleoproteins or mRNA, relying on reagent expression from non-integrated DNA, using novel delivery mechanisms such as viruses or nanoparticles, using unconventional selection methods to avoid integration of transgenes, and/or avoiding tissue culture altogether. (frontiersin.org)
For successful transgene expression, viruses administered into muscle must undergo a series of processes, including host cell interaction and internalization, intracellular sorting, long-range retrograde axonal transport, endosomal liberation, and nuclear import. (frontiersin.org)
The choice of viral vector depends on the efficiency of transgene expression, ease of production, safety, toxicity, and stability. (kaloramainformation.com)
Molecular characterization of the T 2 transgenics using PCR, RT-PCR and Southern blot analyses further revealed the integration, expression and inheritance of the transgene. (ias.ac.in)
We show that injection of rAAV derived from an Iodixanol gradient can be used for in vivo gene transfer applications in the brain and spinal cord without detectable cytopathic effects and directing stable transgene expression for at least 2 months. (nih.gov)
The directed expression of Buffy in the dopamine producing neurons, via aDdc-Gal4 transgene, resulted in flies with increased climbing ability and enhanced survival, while the inhibition of Buffy in the dopaminergic neurons reduced climbing ability over time prematurely, similar to the phenotype observed in the alpha-synuclein-induced Drosophila model of PD. (sdbonline.org)
The expression of human α-synuclein transgene in neuronal populations that include dopamine producing neurons under the control of Ddc -Gal4 produces a robust Parkinson disease model, and results in severely reduced lifespan and locomotor dysfunction. (sdbonline.org)
This therapy was well tolerated and provided stable long-term expression of FAH in pigs with HT1. (biorxiv.org)
Transgene expression was analysed in Arabidopsis T‐DNA transformants carrying defined numbers and arrangement of different reporter genes. (fu-berlin.de)
High, stable transgene expression was observed in plants containing two copies of the β‐glucuronidase (GUS) gene, two or four copies of the green fluorescent protein (GFP) gene and two, four or six copies of the streptomycin phosphotransferase (SPT) gene. (fu-berlin.de)
Delivering consistent, robust transgene expression, the AAVS1 safe harbor site is a preferred target for gene knock-ins. (reportergene.com)
Insertion at the site has been shown to be safe with no phenotypic effects reported, and the surrounding DNA appears to be kept in an open confirmation, enabling stable expression of a variety of transgenes. (reportergene.com)
Additionally, lingering expression or re-expression of viral transgenes in iPSC-derived cells can interfere with their differentiation potential. (addgene.org)
Stable cell lines expressing recombinant proteins are generated by experienced scientists working on protein engineering, expression, and purification, and cell line development services for decades. (welgen.com)
Stable cell lines developed with our proprietary protein expression system with multiple selectable markers is very productive in expressing target proteins. (welgen.com)
CHO suspension clonal stable cell lines are generated by electroporation using proprietary mammalian expression vector system with multiple selectable markers to express single gene of interest. (welgen.com)
Amongst the technologies available are the well-known UCOE ® and SGE® technologies, developed respectively by Merck Millipore and Selexis, that allow to minimize the number of stable clones for which the transgene insertion locus would hinder the expression. (gtp-bioways.com)
Somatic1
Transgenesis will therefore change the germ cells, not only the somatic cells, in order to ensure that the transgenes are passed down to the offspring when the organisms reproduce. (wikipedia.org)
Genes2
In none of the cases analysed were tandem repeat arrangements of transgenes and/or inverted repeat (IR) T‐DNA structures sufficient to trigger silencing of the different reporter genes. (fu-berlin.de)
An important milestone of reprogramming is the silencing of reprogramming transgenes and the up-regulation of corresponding endogenous pluripotency genes. (addgene.org)
Transgenesis1
The introduction of a transgene, in a process known as transgenesis, has the potential to change the phenotype of an organism. (wikipedia.org)
Sequences2
These are typically combined in a bacterial plasmid and the coding sequences are typically chosen from transgenes with previously known functions. (wikipedia.org)
Thus, the mere presence of multiple promoter and/or transgene sequences did not result in gene silencing. (fu-berlin.de)
Genetically2
Viral vectors are genetically stable, non-toxic for host cells and safe to handle. (kaloramainformation.com)
Using mice they had genetically engineered to mimic the human condition, scientists from Notre Dame's W.M. Keck Center for Transgene Research recently identified the mechanism. (nd.edu)
Locus1
Fhb1 , a quantitative trait locus discovered in Chinese germplasm, provides the most stable and the largest effect on FHB resistance in wheat. (nature.com)
Promoter2
The transgene must contain a promoter, which is a regulatory sequence that will determine where and when the transgene is active, an exon, a protein coding sequence (usually derived from the cDNA for the protein of interest), and a stop sequence. (wikipedia.org)
All transgenes were placed under the control of the strong constitutive CaMV 35S promoter. (fu-berlin.de)
Transient1
Here we report a simple and reliable Agrobacterium -mediated method for generating transient and stable transformants of mature C. richardii gametophytes. (biomedcentral.com)
Transformation1
Stable transformation was achieved at a rate of nearly 3% by regenerating entire gametophytes from single transformed cells from T 0 gametophytes on selective media. (biomedcentral.com)
Gene Therapy1
Gene therapy viruses are non-replicating, but still hijack host cell machinery to express transgenes of interest in the nucleus. (frontiersin.org)
Crops2
Transgene escape has been documented for GMO crops since 2001 with persistence and invasiveness. (wikipedia.org)
In some crops, the transgenes are routinely segregated away during meiosis by performing crosses, and thus only a minor concern. (frontiersin.org)
Vectors1
Retroviral vectors are prone to incomplete silencing of reprogramming transgenes, which leads to incomplete reprogramming. (addgene.org)
Integration3
This review highlights diverse strategies to deliver CRISPR/Cas gene editing reagents to regenerable plant cells and to recover edited plants without unwanted integration of transgenes. (frontiersin.org)
Since, the transgene is integrated into the cells of already differentiated tissues, the T 0 plants were chimeric and stable integration was observed in T 1 generation. (ias.ac.in)
Using classic transfection techniques followed by genetic selection for the generation of your recombinant cell line is a time-consuming process often leading to an unpredictable outcome as transgene integration is a rare, random event. (gtp-bioways.com)
Neurons1
Inhibition of parkin or Pink1 through the induction of stable RNAi transgene in the Ddc-Gal4-expressing neurons results in such phenotypes to model PD. (sdbonline.org)
Clones1
Omitting the plasmid backbone, multiple transposable elements carrying the transgene cassette are specifically inserted resulting in highly stable and high expressing clones. (probiogen.de)
Shuttle2
T316992 TAg Stable Y-LIZ Wildtype Mouse Embryonic Fibroblast Cell Line carry the lambda-LIZ transgene (Y-LIZ), a shuttle. (topsan.org)
Transposases - enzymes that shuttle transgenes into multiple open DNA sites - are known to facilitate cell line generation in many ways. (probiogen.de)
Cells1
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
Previously1
As we previously stated, NEC and Transgene share a common goal to harness the power of data and develop new targeted therapies in oncology. (nec.com)
High1
We favour the idea that in many cases this may be attributed to the high transgene doses rather than the repeat arrangements themselves. (fu-berlin.de)
Precise1
However, the technique must invariably deliver a precise result that is stable and safe for the environment and the consumer. (frontiersin.org)
Results1
You, though, want to position your transgene where the action is to yield the best results for your product. (probiogen.de)
Active1
Indeed, transgenes are directed to a multitude of such active sites where they still insert randomly, allowing for optimal transcript levels. (probiogen.de)
Cell line development2
Our faster and more predictive screening techniques may significantly increase speed of stable cell line development while improving quality and uniformity. (welgen.com)
Stable transgenics were selected using stringent selection pressure, molecular characterization and stress-specific bio-efficacy studies. (bvsalud.org)
T3178Ad5 pTP Stable 293 (293pTP) Cell Line was established through the introduction of the piggyBac transposon-based vector encoding for Ad5 pTP. (topsan.org)