Stable mobile and stationary phases. Medical search. Frequent questions

Shim-pack UC series was designed with eight types of stationary phases and sizes to meet diverse research and development needs. (shimadzu.com)
By modifying the matrix or bonding different groups, different separation types of stationary phases are formed, which usually include: normal phase, reverse phase, ion exchange, size exclusion, chiral, and other stationary phases. (flashcolumns.com)

Thus, the evaluation of the interfacing is also reported.The CEC work presented in this thesis details the examination of effects of physicochemical properties of different silica based C18 stationary phases on their chromatographic performance in CEC separations for a series of different acidic, neutral and basic type of analytes. (shu.ac.uk)
The chromatographic mode in which the polarity of the modified groups on the matrix is greater than the polarity of the mobile phase is called normal phase chromatography, which relies on the different polarity of the sample to distribute between the stationary phase and the mobile phase to achieve separation. (flashcolumns.com)
We present a new chromatographic method for the HPLC separation of underivatized amino acids using an acidic, aqueous mobile phase in conjunction with a mixed-mode stationary phase that can be interfaced with the LC IsoLink for compound-specific delta13C analysis. (ox.ac.uk)
Preparation of such large diameter columns is difficult using conventional slurry packing wherein a suspension of stationary phase is forced or "packed" into a chromatographic column using a high pressure stream of liquid to produce a homogeneous and stable column structure. (justia.com)

Learn to capitalize on the advantages of both core-shell and thermally modified fully porous stationary phase media to expand the options for HPLC and UHPLC method development. (phenomenex.blog)
Kinetex Core-Shell particles are available in 1.3, 1.7, 2.6, 3.5, and 5 μm sizes suitable for UHPLC to preparative HPLC, and utilize a variety of bonded phases for standard reversedphase. (phenomenex.blog)
Among the most common methods of enantioseparation is high-performance liquid chromatography (HPLC) on chiral stationary phases. (sciendo.com)
The traditional bonding techniques used to manufacture silica-particle-based HPLC stationary phases provide many benefits, but can emerging technologies tackle unmet needs? (chromatographyonline.com)
This article will review historical bonding techniques still in use for manufacturing HPLC stationary phases today, and also examine some emerging technologies that may be able to tackle unmet needs in novel platforms and phase construction. (chromatographyonline.com)
In 1973, Locke correctly predicted that organosilanes would transform HPLC columns via chemically-bonded phases, mentioning that polymerization of silanes would be the synthetic route taken in general with chemical reactions being carried out to produce a primary organosilane layer (5). (chromatographyonline.com)
The scope of compound-specific stable isotope analysis has recently been increased with the development of the LC IsoLink which interfaces high-performance liquid chromatography (HPLC) and isotope ratio mass spectrometry (IRMS) to provide online LC/IRMS. (ox.ac.uk)
High efficiency separations at any pH The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any mobile phase or pH. (thomassci.com)
The 2D-HPLC system incorporated strong cation exchange and reversed phase separation modes. (cdc.gov)

Affinity chromatography has the advantage of specific binding interactions between the analyte of interest (normally dissolved in the mobile phase), and a binding partner or ligand (immobilized on the stationary phase). (wikipedia.org)
In a typical affinity chromatography experiment, the ligand is attached to a solid, insoluble matrix-usually a polymer such as agarose or polyacrylamide-chemically modified to introduce reactive functional groups with which the ligand can react, forming stable covalent bonds. (wikipedia.org)
Binding to the solid phase may be achieved by column chromatography whereby the solid medium is packed onto a column, the initial mixture run through the column to allow settling, a wash buffer run through the column and the elution buffer subsequently applied to the column and collected. (wikipedia.org)
When conducting analysis with the Nexera UC supercritical fluid chromatography system, because diffusion of the sample band in the mobile phase is high compared with liquid chromatography, separation behavior changes significantly depending on the types of columns used. (shimadzu.com)
Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. (springer.com)
Normal or reversed-phase liquid chromatography can be used for analysis. (springer.com)
In normal phase liquid chromatography (NP-LC), the stationary phase is more polar than the mobile phase. (springer.com)
For historical reasons, it has been reported that HILIC is a variant of normal phase liquid chromatography, but the separation mechanism used in HILIC is more complicated than that in NP-LC. (springer.com)
For example, it is suitable for analyzing compounds in complex systems that always elute near the void in reserved-phase chromatography. (springer.com)
The principle in gas chromatography involves the separation of the components of the sample under test due to partition in between gaseous mobile phase and stationary liquid phase. (cifa.nic.in)
In terms of stationary and mobile phases it is categorized under gas-liquid type of chromatography i.e. stationary phase is a liquid layer supported over a stationary phase while the mobile phase is an inert and stable gas. (cifa.nic.in)
It combines the simplest capillary electrophoresis mode where separations are based on the differences in the electrophoretic migration of charged analytes under the influence of a high electric field with separation based on analyte partitioning between the mobile phase and stationery phase from liquid chromatography. (shu.ac.uk)
Get outstanding peak shapes in reversed phase, ion exchange, HILIC or normal phase chromatography. (fishersci.com)
Gas chromatography technique is to use the difference of distribution coefficients of different compounds in the mobile phase (carrier gas) and stationary phase at a certain temperature to make different compounds flow out in the column successively according to time, to achieve the purpose of separation and analysis. (antiteck.com)
The stationary phase is also commonly known as the packing, which is the core part of chromatography. (flashcolumns.com)
After modification, it has a wide range of applications in the separation and purification of biomolecules such as proteins and carbohydrates in ion exchange chromatography, size exclusion chromatography, and reversed-phase hydrophobic chromatography. (flashcolumns.com)
Almost 50 years later, the C18 phase is still the reigning champion in the reversed-phase liquid chromatography (RPLC) arena, and many chromatography companies are still bonding using the same or a very similar synthetic approach. (chromatographyonline.com)
According to the United States Pharmacopeia ( USP ), there are 858 C18 liquid chromatography phases registered under code L1 (octadecylsilane chemically bonded to porous silica or ceramic microplates, 1.5 to 10 μm in diameter, or a monolithic rod) (3). (chromatographyonline.com)
In preparative chromatography, a fluid known as the mobile phase is passed under pressure, through a compact bed of particulate material known as the stationary phase. (justia.com)
For the effective use of preparative chromatography, it is essential that the bed of stationary phase be prepared as a compact bed of high uniformity. (justia.com)

This report examines the morphology and performance of two types of stationary phase media( Luna® Omega Thermally Modified Fully Porous Particles and Kinetex® Core-Shell Particles from Phenomenex) and their application in method development work. (phenomenex.blog)
Most chromatographers are familiar with conventional fully porous stationary phase material. (phenomenex.blog)

Shim-pack UC series was designed with a variety of stationary phases, making it suitable for analysis of various compounds. (shimadzu.com)
The purpose of this work was to review the options for the characterization of HILIC stationary phases and their applications for separations of polar compounds in complex matrices. (springer.com)
A desirable mobile phase would contain high organic content for better sensitivity and also show good on-column retention for polar ionic compounds. (springer.com)
UC-Diol and UC-Diol II normal phase columns can even be used for lipids and other compounds with low polarity. (shopshimadzu.com)
BIOshell™ IgG 1000 Å C4 columns are highly suited for the reversed phase separation of high molecular weight compounds such as monoclonal antibodies with molecular weight of 150 kDa. (sigmaaldrich.com)

Polar samples always show good solubility in the aqueous mobile phase used in HILIC, which overcomes the drawbacks of the poor solubility often encountered in NP-LC. (springer.com)
These quaternary amine strong anion-exchange columns are highly stable for aqueous and low pH mobile phases. (fishersci.com)

At the same time, the surface of silica gel is easily modified to form other bonded stationary phases. (flashcolumns.com)
There are many kinds of stationary phases modified with a silica gel matrix, and the separation and purification effect is much better than other matrixes. (flashcolumns.com)
However, compared with inorganic substrates such as silica gel, the separation mechanical strength that the organic matrix stationary phase can withstand is lower, and the separation and purification effect is poor, which limits its scope of application in purification. (flashcolumns.com)
The normal phase silica gel stationary phase is mainly used in the preliminary purification of synthetic non-polar or medium-polar intermediates. (flashcolumns.com)
LiChrosorb® RP-8 is a reliable irregular shaped silica gel carriers with reversed phase properties. (thomassci.com)

The retention increases as the polarity of the mobile phase decreases, and thus polar analytes are more strongly retained than nonpolar ones. (springer.com)

The membrane doped with acidic moieties such as 12-phosphotungstic acid shows large protonic conductivities at intermediate temperatures up to 140°C and was found to be flexible as well as thermally stable due to the temperature tolerant inorganic frameworks in the hybrids. (elsevierpure.com)

The mobile phase consisting of 52.5% acetonitrile and 47.5% water provided good peak shape, accuracy and precision (stable ionization). (ubbcluj.ro)

QC lasers are spectroscopically stable and can be operated near room temperature when in pulsed mode. (nist.gov)

While CL increases, the two predominant lipids phosphatidylglycerol (PG) and phosphatidylethanolamine (PE) of the exponential growth phase decrease as B. subtilis enters stationary phase 6 . (nature.com)
We expected to observe similar changes in relative abundances of these major polar lipids as A. radioresistens enters stationary phase. (nature.com)

Although new synthetic approaches have been applied to reversed-phase ligands, the bulk of the commercially available phases are still manufactured via conventional methods (6). (chromatographyonline.com)

Shim-pack UC series offers various stationary phases suitable for the mobile phase of high diffusion and low-viscosity liquid carbon dioxide. (shimadzu.com)
The following process often lends itself to ensuring the quality of glass surfaces during production: Suitable limiting values are first determined in the laboratory using our stationary contact angle measuring instruments, and adherence to these is then checked on site using a mobile unit. (kruss-scientific.com)

The objectives of Phase I are (1) to construct a proof-of-principle generator for operation at 125 kV, (2) to experimentally demonstrate that a uniform, high energy ion beam can be generated, and (3) to develop the conceptual design for a Phase II laboratory prototype DNG system. (nsf.gov)

A wash buffer is then applied to remove non-target biomolecules by disrupting their weaker interactions with the stationary phase, while the biomolecules of interest will remain bound. (wikipedia.org)
The method utilizes a reversed-phase Primesep-A column with embedded, ionizable, functional groups providing the capability for ion-exchange and hydrophobic interactions. (ox.ac.uk)
The surface free energy of glass surface and coating should be such that interactions between the phases ensure good wetting and adhesion. (kruss-scientific.com)

Specialty phases are also available for work at high pH or for separating very polar materials under HILIC conditions. (phenomenex.blog)

No. 3,966,609 does produce highly efficient preparative columns which are stabilized against failure due to stationary phase compaction, the method and apparatus described in the patent do not permit the nondestructive removal of packed columns from the compressive means if it is desired to pack a new column. (justia.com)

The operating table has a new type of wheel suspension and remains stable even on uneven floors with its unique adaptive chassis. (rinicompany.com)

Balance is the ability to move without falling and distribute your weight in order to hold a stable stationary position. (wellnessneuro.com)

Its uniform silica surface and stable chemical modification also ensure high analysis reproducibility. (shimadzu.com)
Spellman presently offers a 20 kW medical Monoblock® for high average power mobile/surgical C-arm applications. (spellmanhv.com)
For larger stationary systems, Spellman's HFe Series is ideal as it balances high average power with the ability to drive large anode 3-phase starter X-Ray tubes and features interfaces for digital imaging workstations. (spellmanhv.com)
It is crucial to maintain a stable electrical contact throughout the CE capillary and ion-source as well as adequate grounding of the high voltage applied in CE. (shu.ac.uk)
Silica supports offer high mechanical strength, allowing the formation of packed beds that are stable for long periods under high operating pressures. (chromatographyonline.com)
ODS-A is a high coverage, fully endcapped C18 phase which exhibits exceptional batch-to-batch reproducibility. (thomassci.com)

Given that normal phase separation is the main separation mode used for SFC, normal phase UC-Diol and UC-Diol II columns are commonly used. (shopshimadzu.com)
The matrix is the basic material composed of the stationary phase, and the commonly used matrix materials are divided into three categories: inorganic, organic, and composite materials. (flashcolumns.com)

The overall objective of the proposed project (Phases I and II) is to demonstrate the feasibility of the DNG concept. (nsf.gov)
Specifically, an optical security system embodying phase-encoding will be conceptualized as encryption means in conjunction with biometric measurements, with the general objective of producing an automatic, tamper-proof security system. (nsf.gov)
The study of separability of the prepared racemates into individual enantiomers R and S using two stationary phases and varying compositions of the mobile phase was another research objective. (sciendo.com)
The main Phase 1 research objective is to demonstrate the required measurement stability (one part per thousand). (nist.gov)

The stationary phase is first loaded into a column to which the mobile phase is introduced. (wikipedia.org)
Sometimes a hybrid method is employed such that the binding is done by the batch method, but the solid phase with the target molecule bound is packed onto a column and washing and elution are done on the column. (wikipedia.org)
The solid phase particles are placed in a column where liquid phase is pumped in from the bottom and exits at the top. (wikipedia.org)
The gravity of the particles ensure that the solid phase does not exit the column with the liquid phase. (wikipedia.org)
A core/shell type analytical column (50×2,1 mm, 2.6 Å) was used with C18 stationary phase. (ubbcluj.ro)
As a sample travels through the column in the mobile phase, it diffuses in and out of the pores, interacts with the stationary phase, and becomes separated. (phenomenex.blog)
Today HAWACH will talk about the connotation of the purification column-stationary phase. (flashcolumns.com)
To determine the stationary phase packed in the Flash purification column, three aspects of the stationary phase must be determined in sequence: matrix type, surface modification/bonding phase, and matrix parameters. (flashcolumns.com)
In the '609 patent a piston containing a filter matrix and fluid sealing mechanism is used to compact a bed of stationary phase and maintain adequate compression to correct column instability. (justia.com)

This polymer's surface contains reactive silanols (Si-OH) that offer a number of possibilities for the synthesis of chemically-bonded phases. (chromatographyonline.com)

The composite matrix is compatible with and neutralizes the advantages and disadvantages of the inorganic matrix and the organic matrix, but the corresponding stationary phase of the composite matrix is generally expensive, so it is less used in preparation and purification. (flashcolumns.com)

The characteristics of the hydrophilic stationary phase may affect and in some cases limit the choices of mobile phase composition, ion strength or buffer pH value available, since mechanisms other than hydrophilic partitioning could potentially occur. (springer.com)

With Reverse Phase, Normal Phase and Specialty Phases, ProteCol ensures you have the right phase for your separation needs. (thomassci.com)

4] A. B. T. Barbaro and P. Degond, Phase transition and diffusion among socially interacting self-propelled agents, Discrete and Continuous Dyn. (degruyter.com)

This Small Business Innovation Research Phase I project involves research on development and application of an optical data acquisition system, I/O devices, and optical data analysis and recognition. (nsf.gov)
The concept will be rapidly prototyped for a proof-of-concept experiment, for feasibility evaluation, and for providing a preliminary design and demonstration plan for Phase II, which will involve development, building, and testing of a brass-board model of the optical security system. (nsf.gov)
This Small Business Innovation Research Phase I project will demonstrate a new class of optical devices that will form the heart of a low-cost, versatile, field-usable environmental monitoring system to be developed in Phase II. (nsf.gov)
Carrier gas system: including gases source, gas purity, gas flow rate controls and measurements.To obtain pure and stable flow rate of carrier gas. (antiteck.com)
Finally, we show that the system exhibits a similar bifurcation to that in Vicsek and Czirok (T. Vicsek, A. Czirók, E. Ben-Jacob, I. Cohen and O. Shochet, Novel type of phase transition in a system of self-driven particles, Phys. (degruyter.com)

In the second part of the paper, the directional headings of the particles are perturbed, in two distinct ways, and the speeds accelerated in order to obtain two distinct classes of non-stationary, complex solutions. (degruyter.com)
Either increasing the variance of the Brownian angular noise, or decreasing the turning rate, or coupling between the particles, cause a similar phase transition. (degruyter.com)

Developed especially for cooling water lines in stationary and mobile applications, Eisele offers the Liquid Line. (ien.eu)

However, given that normal phase columns separate by class, UC-HyP columns may be better suited for separation by molecular type. (shopshimadzu.com)

We studied changes in its lipid composition induced by the onset of stationary phase. (nature.com)

Our mobile measuring equipment enables glass surfaces of any size to be tested non-destructively. (kruss-scientific.com)

It is used in normal-phase purification systems. (flashcolumns.com)
However, it is difficult to modify the surface of alumina, so it is still used in normal phase purification systems at most and a small amount in ion exchange systems. (flashcolumns.com)
In Phase 1, Boston MicroSystems will leverage from work on previous and ongoing FAA, ATF and NSF programs to test three already developed devices, microresonators, SAWs and FPWs, for their applicability as in situ fluid viscosity sensors for condition based maintenance of compressors in refrigerant systems. (nist.gov)
Proton exchange membrane fuel cells (PEMFCs) have been considered to be a key technology for renewable energy systems such as an alternative battery for electric vehicles (EVs), stationary power generators, and mobile phones. (elsevierpure.com)

No. 4,549,584, permits packed columns to be removed from the compressive equipment, additional potentially damaging operations such as trimming or shaping of the stationary phase bed and attachment of end fittings are required to finish the preparation of the columns. (justia.com)

The ratio of polar and non-polar (disperse) interaction parts of the surface tension of the two phases is decisive for the adhesion of the coating. (kruss-scientific.com)

Video Demonstration of the Phase I Prototype: http://youtu.be/AIGigEXn12Q Purpose: This project team will develop and test Zaption, a mobile and desktop platform designed to support educators in effectively and efficiently utilizing video (e.g., from YouTube, Vimeo, or their own desktop) as an interactive teaching and learning object. (sbir.gov)
Video Demonstration of the Phase I Prototype: http://youtu.be/P74Z5oNAo4c Purpose: This project will develop and test Kiko's Thinking Time, a series of game apps designed to strengthen children's cognitive skills related to executive functioning and reasoning. (sbir.gov)

The material to be separated into constituent components, known as the sample, is passed through the stationary phase by means of the mobile phase and is separated into its components by means of differential interaction with the stationary phase. (justia.com)

A good balance exercise routine added to a regular exercise regimen will help seniors stay mobile longer. (wellnessneuro.com)

This stability will be converted to accuracy in Phase 2 using the ozone ultraviolet absorption standard and chemical titration to transfer this standard to NO and NO2. (nist.gov)

Kaishan brand BOREAS series stationary screw air compressor The Boreas(BK) series compressor represent a giant leap in design and performance with each component designed for reliability and ease of maintenance. (industryaircompressor.com)

The operating table is used both stationary in the operating room or as a mobile unit in line with "roll-in roll-out" method. (rinicompany.com)
Jobsite table saws are invaluable for being easy to carry to the job site and give you the same cutting accuracy as a stationary table saw. (woodworknation.com)

Sample pretreatment methods often include liquid-liquid extraction followed by solid phase extraction (SPE), but methods without SPE have also been proposed [ 13 ]. (hindawi.com)

Mobile sources always remain agnostic of multicast mobility operations. (rfc-editor.org)
Operations of PIM in Phase Three (Shortest-Path Tree) 17 4.3.5 . (rfc-editor.org)

Anatomy6

Organisms26

Diseases1

Chemicals and Drugs100

Analytical, Diagnostic and Therapeutic Techniques and Equipment47

Phenomena and Processes72

Disciplines and Occupations1

Technology, Industry, Agriculture4

Information Science9

Health Care7

Types of stationary phases2

Chromatographic4

HPLC9

Chromatography20

Fully porous2

Compounds5

Aqueous2

Silica gel5

Polarity1

Thermally1

Acetonitrile1

Temperature1

Lipids2

Conventional1

Suitable2

Laboratory1

Interactions3

HILIC1

Preparative1

Suspension1

Distribute1

High6

Commonly2

Objective4

Column9

Synthesis1

Inorganic1

Characteristics1

Reverse phase1

Diffusion1

System5

Particles2

Applications1

Type1

Composition1

Size1

Systems4

Columns1

Surface1

Prototype2

Material1

Good1

Stability1

Performance1

Table2

Sample1

Operations2