Stability of rna. Medical search. Frequent questions

In addition, detection of viral RNA by reverse transcription--polymerase chain reaction (RT-PCR), usually in a throat swab or urine sample, and subsequent genotyping of strains is valuable for diagnosis and molecular epidemiology ( 2 ). (cdc.gov)
mRNA is created during the process of transcription, where an enzyme (RNA polymerase) converts the gene into primary transcript mRNA (also known as pre-mRNA). (wikipedia.org)
During transcription, RNA polymerase makes a copy of a gene from the DNA to mRNA as needed. (wikipedia.org)
One notable difference is that prokaryotic RNA polymerase associates with DNA-processing enzymes during transcription so that processing can proceed during transcription. (wikipedia.org)
Shortly after the start of transcription, the 5' end of the mRNA being synthesized is bound by a cap-synthesizing complex associated with RNA polymerase. (wikipedia.org)
Consistent with this notion, stability of the ribosomal DNA (rDNA) repeats of budding yeast requires processes that counteract RNA polymerase II (RNAPII) function at intergenic spacers within the repeats. (mcgill-cihr-ig.ca)
Here we show, however, that RNA polymerase II (Pol II) inside human nucleoli operates near genes encoding rRNAs to drive their expression. (nature.com)
87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)
In particular, we investigate: how the efficiency of RNA polymerase elongation can influence the distribution of the SMC complex condensin during mitotic chromosome condensation and, how the transcription by-product R-loop can interfere with chromosome integrity. (ens-lyon.fr)
Using genetics and genome editing strategies in fission yeast, we have accumulated compelling evidence that the mechanisms of transcription termination at the 3' end of genes play a role in determining the local occupancy of condensin, whatever the RNA polymerase involved. (ens-lyon.fr)
Engineered to carry the λDE3 lysogen that contains the T7 RNA polymerase gene under control of the lac UV5 promoter, these cells are pre-aliquoted in a rack containing 12 strips of 8 wells to increase productivity for throughput bacterial transformations. (thermofisher.com)
IPTG is required to induce expression of the T7 RNA polymerase cascade system that in turn transcribes the T7 promoter regulated target gene. (thermofisher.com)

In general, these protocols aim to enrich for mature mRNA by selection of polyadenylated RNA or by depletion of ribosomal RNA. (nature.com)
The translation of codons into amino acids requires two other types of RNA: transfer RNA, which recognizes the codon and provides the corresponding amino acid, and ribosomal RNA (rRNA), the central component of the ribosome's protein-manufacturing machinery. (wikipedia.org)
Existing models suggest that RNA polymerases I and III (Pol I and Pol III) are the only enzymes that directly mediate the expression of the ribosomal RNA (rRNA) components of ribosomes. (nature.com)
These kits are able to purify all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA), depending on the age of the FFPE tissue as fragmentation of the RNA is known to occur over time. (norgenbiotek.com)
DEAD-box helicase DDX27 regulates 3' end formation of ribosomal 47S RNA and stably associates with the PeBoW-complex. (nih.gov)

Overall, our findings reveal intersecting roles for Pbp1/Ataxin2 and the extracellular environment in the suppression of nucleic acid structures threatening genome stability. (mcgill-cihr-ig.ca)
In the Yildirim lab, we study how epigenetic mechanisms, particularly those that are mediated by long noncoding RNAs (ncRNAs), complement gene expression, impact genome stability and define cell fate decisions. (duke.edu)
Our laboratory uses primarily XCI as a model to understand how long ncRNAs 1) regulate gene dosage and maintain epigenetic state, 2) impact genome stability, and 3) participate in cell fate decisions. (duke.edu)

Here we present a computational approach called exon-intron split analysis (EISA) that measures changes in mature RNA and pre-mRNA reads across different experimental conditions to quantify transcriptional and post-transcriptional regulation of gene expression. (nature.com)
A recently discovered dimension of post-transcriptional gene regulation involves co-regulatory crosstalk between RNA transcripts, which compete for common pools of microRNA (miRNA) molecules. (biomedcentral.com)
lncRNA HOX transcript antisense RNA (HOTAIR) represses gene expression through recruitment of chromatin modifiers. (biomedcentral.com)
In molecular biology, messenger ribonucleic acid (mRNA) is a single-stranded molecule of RNA that corresponds to the genetic sequence of a gene, and is read by a ribosome in the process of synthesizing a protein. (wikipedia.org)
Modulation of RNA stability regulates gene expression in two opposite ways: through buffering of RNA levels upon global perturbations and by supporting adapted differential expression. (scilifelab.se)
Here, we explore the role of RNA stability in achieving balanced gene expression and present genome-wide RNA stabilities in Drosophila melanogaster male and female cells as well as male cells depleted of proteins essential for dosage compensation. (scilifelab.se)
We identify two distinct RNA-stability mediated responses involved in regulation of gene expression. (scilifelab.se)
The first of these responds to acute and global changes in transcription and thus counteracts potentially harmful gene mis-expression by shifting the RNA stability in the direction opposite to the transcriptional change. (scilifelab.se)
The second response enhances inter-individual differential gene expression by adjusting the RNA stability in the same direction as a transcriptional change. (scilifelab.se)
Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)
Besides their canonical roles in translation, tRNAs also originate tRNA-derived small RNAs (tsRNAs), a class of small non-coding RNAs with regulatory functions ranging from translation regulation to gene expression control and cellular stress response. (mdpi.com)
This comprehensive global TSS mapping atlas provides a valuable resource for RNA biology and gene expression analysis in the Enterococci. (frontiersin.org)
Mechanically, m6A can be recognized by "reader" proteins or can directly modify RNA conformation, and it widely affects gene expression by mediating RNA stability, translation, splicing and export. (scienceopen.com)
Our findings therefore suggest that RNA decoration by m(6)A has a fundamental role in regulation of gene expression. (scienceopen.com)
This includes, 1) how small RNAs regulate gene expression in the male germline to support spermatogenesis and fertility, 2) how small RNAs are packaged into mature sperm, 3) how RNAs transmitted during fertilization are able to regulate early embryonic gene expression and development, and 4) how this regulation can alter developmental programs to produce a non-genetically inherited phenotype. (upenn.edu)
MultiShot StripWell BL21 Star (DE3) Competent Cells offer enhanced mRNA stability due to a mutation in the RNaseE gene ( rne 131) that reduces levels of endogenous RNases and mRNA degradation, thereby increasing the stability of mRNA transcripts and increasing protein yield. (thermofisher.com)
In this section, we comprehensively analyzed the expression levels of fourteen m 6 A RNA methylation regulators and clinical factors in patients with HCC from the ICGC (International Cancer Genome Consortium, https://icgc.org/ ), Gene Expression Omnibus (GEO, https://www.ncbi.nlm.nih.gov/geo/ ), and TCGA (The Cancer Genome Atlas, http://cancergenome.nih.gov/ ) databases. (hindawi.com)
Using a small noncoding RNA, microRNA 211, and tools that track the stability and decay of the protein-coding and noncoding RNAs in lab-grown melanoma cells, a team led by a Johns Hopkins Kimmel Cancer Center researcher identified highly unstable RNA molecules in human melanomas , including a novel miR-211 target gene DUSP3. (news-medical.net)
This gene provides instructions for making a particular type of RNA, a molecule that is a chemical cousin of DNA. (medlineplus.gov)
De Novo Variants in CNOT1, a Central Component of the CCR4-NOT Complex Involved in Gene Expression and RNA and Protein Stability, Cause Neurodevelopmental Delay. (bvsalud.org)
CNOT1 is a member of the CCR4-NOT complex, which is a master regulator, orchestrating gene expression , RNA deadenylation, and protein ubiquitination . (bvsalud.org)

A 5' cap (also termed an RNA cap, an RNA 7-methylguanosine cap, or an RNA m7G cap) is a modified guanine nucleotide that has been added to the "front" or 5' end of a eukaryotic messenger RNA shortly after the start of transcription. (wikipedia.org)
Polyadenylation is the covalent linkage of a polyadenylyl moiety to a messenger RNA molecule. (wikipedia.org)
Lawyer Tom Renz has exposed that the COVID-19 vaccines, widely advertised as mRNA (messenger RNA) vaccines, are in fact lab-created hybrids known as modRNA. (republicbroadcasting.org)
They claimed the COVID-19 vaccines were mRNA & that meant MESSENGER RNA (which occurs in life everywhere). (republicbroadcasting.org)
modRNA (modified messenger RNA) is a synthesized form of mRNA that has been altered at specific sites. (republicbroadcasting.org)
Pfizer admitted that during its clinical studies, participants aged 16 years and older received 30 mcg of nucleoside-modified messenger RNA (modRNA) . (republicbroadcasting.org)
Nucleoside-modified messenger RNA (modRNA) is a modified form of mRNA that encodes the spike (S) glycoprotein of the SARS-CoV-2 virus, the virus that causes COVID-19. (republicbroadcasting.org)
Messenger RNA (mRNA) is a single-stranded molecule naturally present in all of our cells. (medicalnewstoday.com)
Although biochemical studies indicate that N(6)-methyladenosine (m(6)A) is the most prevalent internal modification in messenger RNA, an in-depth study of its distribution and functions has been impeded by a lack of robust analytical methods. (scienceopen.com)
This was related to an increase in TF messenger RNA (mRNA) stability. (imperial.ac.uk)

Defects in the regulation of RNA metabolism were also seen in neurodegenerative disorders by involving the mutation or mis-localization of RNA binding proteins leading to the formation of aggregation and inclusion of the proteins. (frontiersin.org)
Using formalin to fix tissues leads to crosslinking of the RNA and proteins, and the process of embedding the tissue samples can also lead to fragmentation of the RNA over time. (norgenbiotek.com)

Decreased expression of the RNA-binding protein, Pumilio, stabilizes predicted neural mRNA targets and presence of a 3'UTR PRE is sufficient to trigger mRNA decay in the nervous system. (escholarship.org)
These are removed in the process of RNA splicing, leaving only exons, regions that will encode the protein. (wikipedia.org)
Mature mRNA is then read by the ribosome, and, utilising amino acids carried by transfer RNA (tRNA), the ribosome creates the protein. (wikipedia.org)
The yeast Ataxin-2 protein Pbp1 binds intergenic RNA, represses RNA:DNA hybrids, and prevents aberrant rDNA recombination/replication. (mcgill-cihr-ig.ca)
Since all of the factors that affect the formation of an RNAs 3D structure cannot be determined experimentally, statistically derived potential energy has been used in the prediction of protein structure. (tmu.edu.tw)
Our findings revise theories of labour division between the major RNA polymerases, and identify nucleolar Pol II as a major factor in protein synthesis and nuclear organization, with potential implications for health and disease. (nature.com)
The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. (wiley-vch.de)
Ribonucleoprotein complex immunoprecipitation and biotinylated RNA pull-down assays demonstrated that PARP-14 forms a complex with the mRNA-destabilizing protein tristetraprolin (TTP) and a conserved adenylate-uridylate-rich element in the TF mRNA 3′ untranslated region. (imperial.ac.uk)
Mauno Vihinen is well-known for his experience and interest in investigating variations and their effects whether they emerge at molecular levels (DNA, RNA protein), in structural context or in the cellular networks and pathways. (lu.se)
He has contributed significantly to the state-of-the-art by frequently presenting novel ideas, methods and results and have pioneered several important proof-of-concepts relevant for the project, including the conceptualization of DNA, RNA and protein variation to Variation Ontology (Vihinen, Genome Res . (lu.se)
n=82) was determined by RNA sequencing and osteomodulin protein levels by immunohistochemistry (n=45) in carotid plaques obtained by endarterectomy from patients. (lu.se)
n=82) was determined by RNA sequencing and osteomodulin protein levels by immunohistochemistry (n=45) in carotid plaques obtained by endarterectomy from patients with or without cerebrovascular symptoms from the CPIP (Carotid Plaque Imaging Project) cohort, Skåne University Hospital, Sweden. (lu.se)

EISA reveals both transcriptional and post-transcriptional contributions to expression changes, increasing the amount of information that can be gained from RNA-seq data sets. (nature.com)
Transfer RNA (tRNA) molecules contain various post-transcriptional modifications that are crucial for tRNA stability, translation efficiency, and fidelity. (mdpi.com)
Despite their critical implication in worldwide public healthcare, essential and available resources such as deep transcriptome annotations remain poor, which also limits our understanding of post-transcriptional control small regulatory RNA (sRNA) functions in these bacteria. (frontiersin.org)

The RNAi approach conscripts a subset of microRNA (miRNA) processing machinery, allowing exogenously supplied RNA of specific sequence to stimulate turnover of accordingly-specific mRNA [6]. (chromatographytoday.com)
Norgen's FFPE RNA Purification Kits provide a rapid method for the isolation and purification of total RNA (including microRNA) from formalin-fixed paraffin-embedded (FFPE) tissue samples in as little as 1 hour. (norgenbiotek.com)
2019) Transcriptome stability profiling using 5'-bromouridine IP chase (BRIC-seq) identifies novel and functional microRNA targets in human melanoma cells. (news-medical.net)

Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)

Previous work with saliva samples, however, has indicated that some buffers optimized for host nucleic acid stabilization may actually inhibit viral RNA detection ( 7 ) (S.B. Griesemer et al. (cdc.gov)
The DNA/RNA Shield SafeCollect Saliva Collection Kit is a user-friendly saliva collection kit for stabilizing the nucleic acid content of saliva and sputum samples. (zymoresearch.com)
AMPIVIEW™ EGFR (AS) Dig RNA Probes are digoxigenin-labeled RNA probes targeting EGFR nucleic acid (DNA/RNA or RNA) for in situ hybridization in tissues and cells. (enzolifesciences.com)

Common mechanisms underlying aging and neurodegeneration involve dysregulated RNA metabolism and genome instability. (frontiersin.org)
Although dysregulated RNA metabolism and defective DNA damage repair have been linked to aging and neurodegeneration, the molecular mechanisms are not sufficiently investigated, which is critical to develop mechanism-based therapeutic avenues. (frontiersin.org)
Noncoding RNAs (ncRNAs) are noncoding transcripts that take part in epigenetic mechanisms by providing RNA-directed silencing, aiding recruitment of chromatin modifying complexes and in some instances, presenting enhancer-like functions to boost transcription. (duke.edu)
Although macrophage TF expression is known to be regulated at the RNA level, very little is known about the mechanisms involved. (imperial.ac.uk)

Although RNA-seq mostly generates reads that map to exons, it also captures less abundant intronic sequences 6 . (nature.com)
If sequences specifying such intrastrand triplexes are encoded in genomes, the potential exists for the formation of stable structures in RNA or DNA in vivo. (elsevierpure.com)
G-quadruplex (G4) DNA, an alternate structure formed by Hoogsteen hydrogen bonds between guanines in G-rich sequences, threatens genomic stability by perturbing normal DNA transactions including replication, repair, and transcription. (nih.gov)
H1 sequences with fewer than three base-pairs fail to support full activity in vitro or in vivo, arguing against any significant difference in the stability of short RNA helices under in vitro and intracellular conditions. (umassmed.edu)
In the current work, we evaluate the statistical free energy of various secondary structure motifs, including base-pair stacks, hairpin loops, and internal loops, using their statistical frequency obtained from the comparative analysis of more than 50,000 RNA sequences stored in the RNA Comparative Analysis Database (rCAD) at the Comparative RNA Web (CRW) Site. (tmu.edu.tw)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
They form more stable hybrids with complementary RNA strands than equivalent DNA/RNA sequences. (eurogentec.com)

Most of the m 6 A RNA methylation regulators were confirmed to be differentially expressed among groups stratified by clinical characteristics and tissues. (hindawi.com)
In summary, we demonstrated the vital role of m 6 A RNA methylation regulators in the initial presentation and progression of HCC and constructed a nomogram which would predict the clinical outcome and provide a basis for individualized therapy. (hindawi.com)

We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
A better understanding of how these species cause disease and spread antibiotic resistance requires a knowledge of how its genes are controlled, on both the DNA and the RNA level. (frontiersin.org)
The BL21 Star strain has higher basal expression of heterologous genes than BL21 (DE3) strains, due to the increased stability of mRNA. (thermofisher.com)

The inter- and intramolecular forces between this RNA and other small molecules and macromolecules, in addition to other factors in the cell such as pH, ionic strength, and temperature, influence the complex dynamics associated with transition of a single stranded RNA to its secondary and tertiary structure. (tmu.edu.tw)

RNA-seq experiments generate reads derived not only from mature RNA transcripts but also from pre-mRNA. (nature.com)
Other researchers, Perera notes, have attempted to profile genomewide transcript stability by using chemicals that block all transcription, and then observing the stability of the transcripts that existed before blocking. (news-medical.net)
To address this, Perera and colleagues used BRIC-seq, a method that tags transcripts with 5-Bromouridine, which is incorporated into newly synthesized RNAs and then can be immunocytochemically analyzed over time without disturbing the rest of the cellular environment. (news-medical.net)

Accurate laboratory results for detection of IgM and viral RNA are dependent on proper collection, processing, shipment, and storage of clinical samples and use of accurate tests performed by a proficient laboratory. (cdc.gov)
DBS has been used for various epidemiologic studies for the detection of measles- and rubella-specific IgG and IgM antibodies and viral RNA ( 3--5 ). (cdc.gov)
PolyU is a synthetic ssRNA which when complexed to cationic lipids can substitute for viral RNAs in inducing IFN-α production in plasmacytoid dendritic cells (pDC). (invivogen.com)
TLR7 and TLR8 can recognize both self and viral RNA but seem able to distinguish the presence of viral RNA by detecting their abnormal localization in the endosome rather than a particular RNA motif. (invivogen.com)

High Quality and Yield of Total RNA. (norgenbiotek.com)
Total RNA was isolated from one slice of hamster FFPE kidney section (20 micron thickness) using Norgen's FFPE RNA Purification Kit and a leading competitors kit. (norgenbiotek.com)
As it can be seen, Norgen not only isolated higher yields of total RNA, but the RNA was also of a higher quality as evidenced by the higher RIN values obtained with Norgen's RNA. (norgenbiotek.com)
Total RNA and DNA were simultaneously isolated from 50 mL of water sample containing 10 7 cfu/mL E.coli using Norgen's Water RNA/DNA Purification Kit and subsequently run on gels for visual analysis. (norgenbiotek.com)

The steady state levels of RNAs, often referred to as expression levels, result from a well-balanced combination of RNA transcription and decay. (scilifelab.se)
Alterations in RNA levels will therefore result from tight regulation of transcription rates, decay rates or both. (scilifelab.se)
The C-terminal domain of YTHDF2 selectively binds to m6A-containing mRNA whereas the N-terminal domain is responsible for the localization of the YTHDF2-mRNA complex to cellular RNA decay sites. (scienceopen.com)
N6-Methyladenosine (m 6 A), a predominant internal modification of RNA in mammalian cells, has been recognized as having a vital role in mRNA stability, export, translation, splicing, and decay [ 4 ]. (hindawi.com)
So we decided to look at a completely new angle of genomewide RNA stability and decay during the first steps in the development of melanoma. (news-medical.net)

RNA is a notoriously fragile molecule. (medicalnewstoday.com)
RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)

The extensive processing of eukaryotic pre-mRNA that leads to the mature mRNA is the RNA splicing, a mechanism by which introns or outrons (non-coding regions) are removed and exons (coding regions) are joined. (wikipedia.org)

However, virus RNA in nonsupplemented saliva is stable for extended periods and at elevated temperatures. (cdc.gov)
RNA is inherently less stable than DNA due to its chemical structure. (idtdna.com)

Cellular RNAs are regulated at multiple stages, including transcription, RNA maturation and degradation. (nature.com)

The shield prevents Pol I from producing sense intergenic noncoding RNAs (sincRNAs) that can disrupt nucleolar organization and rRNA expression. (nature.com)

Combined with untested concerns regarding SARS-CoV-2 RNA stability in saliva, using supplements to reduce degradation and improve sample processing has become common. (cdc.gov)

We report a process of rDNA repeat stabilization that relies on suppressing intergenic RNA:DNA hybrids. (mcgill-cihr-ig.ca)

We reveal a nucleolar Pol-II-dependent mechanism that drives ribosome biogenesis, identify disease-associated disruption of nucleoli by noncoding RNAs, and establish locus-targeted R-loop modulation. (nature.com)
Traditionally, the nucleolar Pol I and nucleoplasmic Pol III are viewed as the sole mammalian RNA polymerases that directly mediate housekeeping ribosome biogenesis. (nature.com)

More specifically, our experiments indicate that the backtracking of RNA polymerases that occurs naturally at termination sites can drive the accumulation of condensin ( Fig. 2 ). (ens-lyon.fr)

FANCJ belongs to a conserved iron-sulfur (Fe S) cluster family of helicases important for genomic stability including XPD (nucleotide excision repair), DDX11 (sister chromatid cohesion), and RTEL (telomere metabolism), genetically linked to xeroderma pigmentosum/Cockayne syndrome, Warsaw breakage syndrome, and dyskeratosis congenita, respectively. (nih.gov)
To elucidate the role of FANCJ in genomic stability, its molecular functions in G4 metabolism were examined. (nih.gov)

N6-methyladenosine (m6A) is the most abundant inner RNA modification in eukaryotes. (scienceopen.com)

We apply EISA to 17 diverse data sets to show that most intronic reads arise from nuclear RNA and changes in intronic read counts accurately predict changes in transcriptional activity. (nature.com)
Three recent studies based on RNA-seq provided evidence that intronic reads might correlate with transcriptional activity. (nature.com)

Transcription is when RNA is copied from DNA. (wikipedia.org)
RNA stability assay was applied to characterize the mechanism of C1QBP regulating XDH transcription. (medsci.org)

To pursue this question, it is important to determine the stabilities of representative triple helices at physiological temperature and pH. (elsevierpure.com)

Since 2001, LabNet reference laboratories in Australia, Cote d'Ivoire, Netherlands, Turkey, Uganda, the UK, and the United States have been working to 1) determine IgM and RNA stability in DBS and OF samples and 2) optimize the methods for IgM antibody assay and protocols for RNA detection in DBS and OF samples ( 8--10 ). (cdc.gov)
AMPIVIEW™ EGFR (AS) Dig RNA probes are conjugated with digoxigenin and have been optimized to produce clear results with Enzo's DIGX ® anti-digoxigenin linker and nanopolymner-based detection systems, POLYVIEW ® PLUS, combined with HIGHDEF ® chromogens and counterstain to produce clear results that can be visualized with a light microscope. (enzolifesciences.com)
AMPIVIEW™ EGFR (AS) Dig RNA Probes are optimized for detection of EGFR RNA or RNA/DNA in FFPE tissues or cells with DIGX ® anti-digoxigenin linker, POLYVIEW® PLUS detection reagents combined with HIGHDEF ® chromogens and counterstains (linker and detection solutions not inlcuded). (enzolifesciences.com)

They reported their findings June 16 in RNA Biology online. (news-medical.net)
This sledgehammer method, however, is considered toxic, and can alter other aspects of a cell's biology in ways that may obscure transcript stability information. (news-medical.net)

Previous investigations have concluded that while both DNA and RNA can participate in the pyrimidine tripler motif under mildly acidic conditions, these structures are often relatively unstable at neutral pH. (elsevierpure.com)
We are now exploring the stability of intrastrand DNA and RNA pyrimidine motif triplexes at physiological temperature and pH. (elsevierpure.com)
Short intrastrand triplexes were observed to form in the pyrimidine motif in both DNA and RNA. (elsevierpure.com)

Ecology and Evolution in the RNA World Dynamics and Stability of Prebiotic Replicator Systems. (nih.gov)
In a melanoma cell line that normally lacks miR-211, the researchers introduced miR-211 expression and then used the BRIC-seq method to investigate transcriptome stability dynamics in the presence/absence of miR-211. (news-medical.net)

As seen with many other bacteria, RNA-seq technology has now become the method of choice for transcriptome analysis in the enterococcus. (frontiersin.org)
Due to the development of RNA sequencing technology, the distribution pattern of m6A in the transcriptome has been uncovered. (scienceopen.com)

RNA which contains an intron sequence that has an enzyme- like catalytic activity. (genomicglossaries.com)
An extensive repertoire of modifications is known to underlie the versatile coding, structural and catalytic functions of RNA, but it remains largely uncharted territory. (scienceopen.com)

We developed a quantitative assay of hairpin ribozyme cleavage activity in yeast to learn how structure-function relationships defined for RNA enzymes in vitro relate to RNA-mediated reactions in cells. (umassmed.edu)

The RNA is preferentially purified from other cellular components without the use of phenol or chloroform. (norgenbiotek.com)

R-loops are three-stranded structures that form when the nascent RNA hybridizes with its DNA template, leaving the non-template DNA strand unpaired ( Fig. 3 ). (ens-lyon.fr)

Targeted elimination of RNA:DNA hybrids via various methods restores rDNA stability in Pbp1-deficient cells. (mcgill-cihr-ig.ca)

Pellicular anion-exchange chromatography applied to RNAi assays for monitoring strand stoichiometry and RNA stability. (chromatographytoday.com)
aRNA is RNA that is transcribed from the coding, rather than the template, strand of DNA. (genomicglossaries.com)

AMPIVIEW™ EGFR (AS) Dig RNA probes have been designed to target the EGFR or HER-1 DNA and RNA in tissues and cells. (enzolifesciences.com)

2004. Species-specific recognition of single-stranded RNA via toll-like receptor 7 and 8. (invivogen.com)

FFPE RNA Purification Kits (Cat. (norgenbiotek.com)
The Norgen FFPE RNA Purification Kit 25300 enable our laboratory to access the RNA and transcriptomic information from the archived paraffin blocks. (norgenbiotek.com)
Norgen's FFPE RNA Purification Kits provide conditions that allow for the partial reversing of the formalin modifications, resulting in a high quality and yield of RNA. (norgenbiotek.com)
Norgen's FFPE RNA Purification Kit isolates FFPE RNA that exceeds both yield and quality of competitors. (norgenbiotek.com)

The limitation that the researchers aimed to solve was RNA guide instability, which was encountered in earlier applications of the technology and led to low genome labeling efficiency. (isaaa.org)

Long non-coding RNAs (lncRNAs) govern fundamental biochemical and cellular processes. (biomedcentral.com)

To explore the viability of broadly deploying affordable saliva-based surveillance approaches ( 8 ), we characterized SARS-CoV-2 RNA stability and virus infectivity in saliva samples stored in widely available, sterile, nuclease-free laboratory plastic (polypropylene) tubes. (cdc.gov)

PIWI-interacting RNAs (piRNAs) are a large family of small, single-stranded, non-coding RNAs present throughout the animal kingdom. (biomedcentral.com)
The functions of small RNAs in fertility, inheritance, and development. (upenn.edu)
Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. (norgenbiotek.com)

AMPIVIEW™ NSP Dig RNA probes (no signal) detected in prostate hyperplasia tissue specimen with DIGX® rabbit anti-digoxigenin linker (ENZ-ABS303), POLYVIEW® PLUS AP (anti-rabbit) (ENZ-ACC110), combined with HIGHDEF® Red Chromogen/Substrate and Hematoxylin counterstain (ENZ-ACC106). (enzolifesciences.com)
AMPIVIEW™ NSP Dig RNA probes are negative control probes. (enzolifesciences.com)

Water RNA/DNA Purification Kits (Cat. (norgenbiotek.com)
From observing the gels it can be seen that the kit allows for the isolation and purification of high yields of concentrated and high quality RNA and DNA. (norgenbiotek.com)
Fifty mL water samples spiked with increasing amounts of E.coli were filtrated through the provided 0.45 µm water filter columns, and RNase-treated DNA was isolated from the filter by following Norgen's Water RNA/DNA Purification Kit manual. (norgenbiotek.com)

Anatomy13

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