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  • verprolin
  • The WASp family proteins includes WASp, N-WASp, SCAR/WAVE, WHAMM and WASH the five of them share a C- terminal VCA (verprolin, central, acidic) domain where they interact with actin nucleating complex (ARP2/3) and they differ in their terminal domains. (wikipedia.org)
  • actin
  • Deletion of the tail homology 2 (TH2) domain, previously implicated in ATP-insensitive actin binding, has no detectable effect on Myo5p function. (rupress.org)
  • In contrast, myo3Δ myo5Δ cells expressing mutant Myo5 proteins with deletions of the src homology domain 3 (SH3) or both TH2 and SH3 domains display defects including Myo5p patch depolarization, actin disorganization, and phenotypes associated with actin dysfunction. (rupress.org)
  • The first step, assembly of Myo5p patches, is dependent upon F-actin, and the second step, polarization of actin patches, requiresVrp1p and the SH3 domain of Myo5p. (rupress.org)
  • The head or motor domain contains binding sites for ATP and actin. (rupress.org)
  • The Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. (wikipedia.org)
  • phosphopeptide
  • A phosphopeptide library screen has recently been used to deduce an 'optimal' binding sequence for the Lck SH2 domain. (rcsb.org)
  • We report here the high-resolution crystallographic analysis of the Lck SH2 domain in complex with this phosphopeptide. (rcsb.org)
  • reveals
  • Comparison of intron positions demonstrates that extensive intron loss has occurred during invertebrate evolution and also reveals the presence of conserved introns in both the N- and C-terminal PLC-γ SH2 domains that are present in SH2 domains in many other genes. (genetics.org)
  • Sequence analysis of the fusion MLL/EEN transcript in our patient reveals that exon 6 of MLL is fused to the N- terminal end of EEN, a fusion that would create a chimeric protein that includes the major functional domain of EEN. (hku.hk)
  • A detailed bioinformatic examination of SH2 domains of human and mouse reveals 120 SH2 domains contained within 115 proteins encoded by the human genome, representing a rapid rate of evolutionary expansion among the SH2 domains. (wikipedia.org)
  • catalytic
  • PLC-γ is particularly interesting from an evolutionary standpoint, because it has a central region between the X and Y catalytic domains that is unique among PLC subtypes. (genetics.org)
  • 2001
  • each domain is presumed to have arisen once and then been spread both by gene duplication and by being co-opted into existing genes by exon shuffling via retrotransposition, illegitimate recombination, or long interspersed nuclear element-mediated 3′ transduction ( L ong 2001 ). (genetics.org)
  • structures
  • IBE cells with an inactive SH2 domain of Shb displayed disorganised formation of tubular structures in the tube formation assay, while overexpression of wild-type Shb led to accelerated tubular morphogenesis. (diva-portal.org)
  • A large number of SH2 domain structures have been solved and many SH2 proteins have been knocked out in mice. (wikipedia.org)
  • mutant
  • Mutant Myo5 proteins with deletions in nonmotor domains were expressed in myo3Δ myo5Δ cells and the resulting strains were analyzed for Myo5p function. (rupress.org)
  • growth
  • Thus, the SH3 domain of Myo5p contributes to but is not sufficient for localization of Myo5p either to patches or to sites of polarized cell growth. (rupress.org)