• In recent years, extensive research has established that some immune cells either reside in the heart or have very complicated interactions with cardiomyocytes through permanent blood circulation. (biomedcentral.com)
  • Herein, we introduce a cardiac-mimetic cell-culture system that resembles the microenvironment in the heart and provides interactions with cardiomyocytes and electrical cues to the cultured fibroblasts for direct cardiac reprogramming. (thno.org)
  • At its most elementary level, regeneration is mediated by the molecular processes of gene regulation and involves the cellular processes of cell proliferation, morphogenesis and cell differentiation. (wikipedia.org)
  • Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
  • In some cases, "master" genes have been described which control differentiation versus self-renewal. (justia.com)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • The induction of cardiomyocyte differentiation in stem cells is especially useful in developing therapeutic methods and products for heart disease and abnormal heart conditions. (justia.com)
  • Transcription factor GATA4-targeted compounds that have previously shown in vivo efficacy in cardiac injury models were tested for stage-specific activation of atrial and ventricular reporter genes in differentiating pluripotent stem cells using a dual reporter assay. (biomedcentral.com)
  • In this line, reporter gene assays with combinatorial treatment of 3i-1000 and the BET bromodomain inhibitor (+)-JQ1 demonstrated the cooperative role of GATA4 and BRD4 in the modulation of chamber-specific cardiac gene expression. (biomedcentral.com)
  • Collectively, these results indicate the potential for therapeutic alteration of cell fate decisions and pathological gene regulatory networks by GATA4-targeted compounds modulating chamber-specific transcriptional programs in multipotent cardiac progenitor cells and cardiomyocytes. (biomedcentral.com)
  • The natural history of cardiac rhabdomyomas is one of spontaneous regression. (medscape.com)
  • We compared this data with existing murine and 3D cardiac organoid scRNA-seq data and confirmed the dominance of left ventricular cardiomyocytes (>90%) in our hiPSC-derived progeny. (stanford.edu)
  • The myocardial nicheplays a critical role in directing the function and fate of cardiomyocytes;therefore, engineering a biomimetic scaffold holds excellent promise.We produced an electroconductive cardiac patch of bacterial nanocellulose(BC) with polypyrrole nanoparticles (Ppy NPs) to mimic the naturalmyocardial microenvironment. (ibecbarcelona.eu)
  • The human neonatal dermal fibroblasts containing cardiac transcription factors were plated on the membrane and cultured with the murine cardiomyocyte in the presence of the electric stimulus. (thno.org)
  • The cardiac-mimetic culture system may serve as an effective tool for producing a feasible number of reprogrammed cardiomyocytes from fibroblasts. (thno.org)
  • The cardiac patches were robust: they could be lifted out and handled well without losing their shape and the cardiomyocytes within them responded by elongating, aligning and maturing as in the normal heart. (bsgct.org)
  • There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
  • In this review, we focus on the role of cardiomyocyte-derived and cardiac fibroblast-derived microRNAs that are involved in the regulation of genes associated with cardiomyocyte and fibroblast function and in atherosclerosis-related cardiac ischemia. (archivesofmedicalscience.com)
  • Finally, it seems appropriate to consider the "sodium channel syndrome" (mutations in the gene of the α subunit of the sodium channel, SCN5A gene) as a single clinical entity that may manifest in a wide range of phenotypes, to thus have a better insight on these cardiac syndromes and potential outcomes for their clinical treatment. (bvsalud.org)
  • Due to cellular heterogeneity in the heart, the activation of gene programs representing specific atrial and ventricular cardiomyocyte subtypes would be highly desirable. (biomedcentral.com)
  • Furthermore, we conduct a cross-species prediction of cardiomyocyte subtypes from in vitro-derived human induced pluripotent stem cells and unexpectedly uncover a predominance of left ventricular (LV) identity that we confirmed by an LV-specific TBX5 lineage tracing system. (stanford.edu)
  • First, they have both the cell source for pluripotent cardiomyocytes or endothelial cells and the "bioink" vehicle from the same patient. (bsgct.org)
  • The conversion of mature somatic cells into pluripotent stem cells, both by nuclear transfer and transduction with specific 'reprogramming' genes, represents a major advance in regenerative medicine. (ca.gov)
  • This has been driven by positive factors such as the marked improvement in protocols for differentiating pluripotent stem cells (either embryonic or induced pluripotent) into beating cardiomyocytes as well as negative ones such as the high rate of loss when cardiomyocytes are injected as a suspension into the muscle of the heart. (bsgct.org)
  • We generated induced pluripotent stem cells (iPSC) from healthy controls, DM1 and DM2 subjects and differentiated these into cardiomyocytes. (jci.org)
  • The improvement of preclinical cardiotoxicity testing, discovery of new ion-channel-targeted drugs, and phenotyping and use of stem cell-derived cardiomyocytes and other biologics all necessitate high-throughput (HT), cellular-level electrophysiological interrogation tools. (nature.com)
  • Most notably, upon transplantation to the perinatal cerebellum, cerebellar stem cells from all ages are able to acquire the position and mature electrophysiological properties of cerebellar granule cells. (silverchair.com)
  • Stem cells are undifferentiated cells which can give rise to a succession of mature functional cells. (justia.com)
  • Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
  • IPSC-derived cardiomyocytes from DM1 but not DM2 had aberrant splicing of known target genes and MBNL sequestration. (jci.org)
  • MicroRNAs are small non-coding post-translational biomolecules which, when expressed, modify their target genes. (archivesofmedicalscience.com)
  • In the central nervous system, polarized oligodendrocytes form myelin by wrapping processes in a spiral pattern around neuronal axons through myelin-related gene regulation. (koreascience.kr)
  • Since these events occur at a distance from the cell body, post-transcriptional control of gene expression has strategic advantage to fine-tune the overall regulation of protein contents in situ. (koreascience.kr)
  • Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene. (thermofisher.com)
  • Biochemicalanalyses revealed that H9c2 cells showed different cardiomyocyte phenotypesand distinct levels of maturity depending on the amount of Ppy inthe substrate used. (ibecbarcelona.eu)
  • RNA-sequencing from DM1 and DM2 iPSC-derived cardiomyocytes revealed distinct misregulation of gene expression as well as differential aberrant splicing patterns. (jci.org)
  • ABSTRACT Teleost eggs contain an abundant store of maternal thyroid hormones (THs) and early in zebrafish embryonic development all the genes necessary for TH signalling are expressed. (pharmaceuticalintelligence.com)
  • The final result is that, after MI, billions of cardiomyocytes (CMs) with limited proliferation capacity are lost and substituted by heterogeneous collagen-rich fibrotic scar tissue [ 2 ] [ 3 ] . (encyclopedia.pub)
  • Taken together, the findings suggest that induction of adipogenesis in 3D culture provides a more adipocyte-specific gene expression pattern and enhances mitochondrial respiration, resulting in more adipocyte-like cellular properties. (bvsalud.org)
  • These mice have a unique T-cell receptor (TCR) gene in all cells, as opposed to the mixture present from normal TCR gene rearrangement occurring during immune system development. (ca.gov)
  • These mice have prerearranged T-cell receptor (TCR) genes in all cells. (ca.gov)
  • Cells in the primordia of zebrafish fins, for example, express four genes from the homeobox msx family during development and regeneration. (wikipedia.org)
  • We validate optical actuation by virally introducing optogenetic drivers in rat and human cardiomyocytes or through the modular use of dedicated light-sensitive somatic 'spark' cells. (nature.com)
  • Some cells in some multicellular organisms lack mitochondria (for example, mature mammalian red blood cells ). (wikipedia.org)
  • In the heart, cardiomyocytes interact with neighboring cells through direct cell-cell interactions, various secreted cytokines, and electric signals (Figure 1 A) [ 24 ]. (thno.org)
  • Many strategies have been tried, such as mixing in endothelial or other blood vessels cells with the cardiomyocytes and introducing tunnels in the construct with needles to encourage blood vessel formation. (bsgct.org)
  • Although the majority of works on FMRP have been performed in neurons, it is also found in the developing or mature glial cells including oligodendrocytes, where its function is not well understood. (koreascience.kr)
  • Ca2+ transients differed between DM1 and DM2 IPSC-derived cardiomyocytes and each differed from healthy control cells. (jci.org)
  • Myocardial infarction (MI) is one of the clusters of numerous other cardiovascular diseases and occurs due to the blockage of the coronary artery delivering blood (ischemia) to the ventricle with consequent oxygen shortage to the contractile cells (cardiomyocytes) [ 1 ] . (encyclopedia.pub)
  • With over 100 types of monogenic inherited cardiovascular disease (CVD) discovered so far, the application of gene therapy has become a focus for recent CVD research and clinical trials. (cyagen.com)
  • Today, we discuss a research paper published by a client of Cyagen Biosciences as an example to show you the foundational ideas of successful preclinical research on gene therapy for cardiovascular disease. (cyagen.com)
  • Large numbers of cardiomyocytes (tens of millions to billions) can be produced with high purity even in an academic laboratory, and they readily form tissue engineered constructs with synchronous beating. (bsgct.org)
  • Many of the genes that are involved in the original development of tissues are reinitialized during the regenerative process. (wikipedia.org)
  • Cardiovascular diseases often cause substantial heart damage and even heart failure due to the limited regenerative capacity of adult cardiomyocytes. (thno.org)
  • The cardiomyocytes are rather immature, and this can have benefits as they are still able to expand their numbers, are resistant to low oxygen conditions and can integrate more readily into the host heart when grafted. (bsgct.org)
  • MicroRNAs regulate gene expression at the post-transcriptional level by binding to 3′- or less often to 5′-untranslated regions of target messenger RNAs (mRNAs), which in consequence leads to inhibited translation and/or induces degradation of targeted mRNA [ 1 ]. (archivesofmedicalscience.com)
  • Here, we describe the development of a TBX5/MYL2 lineage tracing reporter system that allows for the identification of FHF- progenitors and their descendants including left ventricular cardiomyocytes. (stanford.edu)
  • 873 During the developmental process, genes are activated that serve to modify the properties of cell as they differentiate into different tissues. (wikipedia.org)
  • Pharmacological modulation of cell fate decisions and developmental gene regulatory networks holds promise for the treatment of heart failure. (biomedcentral.com)
  • In this study, we evaluated gene expression patterns using RNA sequencing and metabolic functions using an extracellular flux analyzer in 3T3-L1 preadipocytes with and without adipogenic induction in 2D culture and 3D culture. (bvsalud.org)
  • Consistently, expression levels of mitochondria-encoded genes including mt-Nd6, mt-Cytb, and mt-Co1 were significantly increased by adipogenic induction of 3T3-L1 preadipocytes in 3D culture compared with those in 2D culture. (bvsalud.org)
  • Chemically induced gene expression changes were characterized by qRT-PCR, global run-on sequencing (GRO-seq) and immunoblotting, and the network of cooperative proteins of GATA4 and NKX2-5 were further explored by the examination of the GATA4 and NKX2-5 interactome by BioID. (biomedcentral.com)
  • Reporter gene assays were conducted to examine combinatorial effects of GATA-targeted compounds and bromodomain and extraterminal domain (BET) inhibition on chamber-specific gene expression. (biomedcentral.com)
  • GATA4-targeted compounds 3i-1000 and 3i-1103 were identified as differential modulators of atrial and ventricular gene expression. (biomedcentral.com)
  • We showed previously that cytoplasmic release of mtDNA activates the cGAS STING TBK1 pathway resulting in interferon-stimulated gene (ISG) expression that promotes antiviral immunity4. (regenerativemedicine.net)
  • Here, we find that persistent mtDNA stress is not associated with basally activated NF-κB signalling or interferon gene expression typical of an acute antiviral response. (regenerativemedicine.net)
  • These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins (1, 2). (novusbio.com)
  • Surprisingly, approximately 50% of these mice develop spontaneous T cell lymphomas, which originate in the thymus. (ca.gov)
  • Surprisingly, approximately 50% of mice with prerearranged TCR genes develop spontaneous T cell lymphomas, which originate in the thymus. (ca.gov)
  • We herein demonstrated that circadian clock network in the intestines plays pivotal roles in skeletal metabolism such that the lack of Bmal1 gene in the intestines (Bmal1Int-/- mice) caused bone loss with bone resorption being activated and bone formation suppressed. (jci.org)
  • Gene therapy has given many patients with congenital diseases new hope! (cyagen.com)
  • In recent years, several gene therapy drugs have been approved for marketing around the world, and many more are advancing through clinical trials. (cyagen.com)
  • The birth of a gene therapy drug is based on performing sufficient basic research with both innovation and integrity to be used for pre-clinical research and clinical trials. (cyagen.com)
  • Currently required preclinical cardiotoxicity testing (part of the drug development process, Fig. 1 ) specifically focuses on a drug's blocking action on the hERG K + channel, which provides one of the main repolarizing currents in cardiomyocytes. (nature.com)
  • Characterization of these airborne strains and investigation of their potential disease-causing genes could facilitate identification of environmental sources of contamination leading to foodborne illnesses and nosocomial infections transported by air. (academic-accelerator.com)
  • Line-scanning analysis of the fluorescence intensity suggested that TβRIII was mainly present in the vicinity of the plasma membrane of mature cardiomyocytes (Figure 1F, b). (cyagen.com)
  • Polymorphisms in UGT genes have been linked to excretion of steroids and risk of some cancers. (helsinki.fi)
  • Nano-thin and nano-porous membranes in the culture system facilitated interactions between fibroblasts and cardiomyocytes in coculture. (thno.org)