Splicing intronic micro rna. Medical search. Frequent questions

Interestingly, it has been shown that platelets contain a broad spectrum of RNA molecules, including, in addition to mRNAs and miRNAs, also pre-mRNAs and a role of mRNA splicing in regulation of platelet protein synthesis has been proposed 18 , 19 . (nature.com)
Initially, two observations prompted the present study: i) previous results revealed down-regulation of p68 RNA helicase resulting from overexpression of the IDX hairpin loop in HeLa cells, and ii) the secondary structure of the IDX hairpin loop resembles pri-miRNAs, implying that an miRNA could be processed from the hairpin loop-containing pri-miRNA and regulate 68 RNA helicase. (lidsen.com)
microRNAs (miRNAs). (unitn.it)
Intensive investigations have identified a collection of microRNAs (miRNAs) and their functional machineries in cytoplasm. (biomedcentral.com)
In this study, the miRNAs and their target mRNAs in the Argonaute2 (Ago2) complex of nucleus and cytoplasm of gastric cancer cells were characterized using high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation (HITS-CLIP). (biomedcentral.com)
Thus, it has become increasingly important to develop biochemical tools based on physical interactions between miRNAs and their specific target RNAs. (biomedcentral.com)
Recently, there has been strong interest in exploring the roles of non-coding RNAs, including circular RNAs (circRNAs), long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), in the process of GC [ 2 - 4 ]. (archivesofmedicalscience.com)
Moreover, circRNAs have four major functions, including as competing endogenous RNAs (ceRNAs) to sponge microRNAs (miRNAs), interaction with RNA binding proteins to regulate the cell cycle and proliferation and the management of gene transcription and protein translation [ 8 , 9 ]. (archivesofmedicalscience.com)
miRNAs are small, non-coding and single-stranded RNAs (approximately 22 nucleotides) and function as post-transcriptional regulators to modulate gene expression by sequence-specific interaction with their 3′-untranslated regions (3′-UTRs) [ 14 ]. (archivesofmedicalscience.com)
Unlike miRNAs whose central function is to restrain mRNA translation by inducing degradation, lncRNAs can act as numerous roles to exert their functions by directly or indirectly interacting with DNA, RNA or protein, even can encode some short peptides (Fig. 3 a-d) [ 6 ]. (biomedcentral.com)
It is generally recognized that most of the mammalian genome is transcribed into ncRNAs, including microRNAs (miRNAs) and long ncRNAs (lncRNAs). (biomedcentral.com)
SncRNAs are less than 200 nt in length, consisting of microRNAs (miRNAs), Piwi-interacting RNAs (piRNAs), endogenous small interfering RNAs (endo-siRNAs or esiRNAs) and transcription initiation RNAs (tiRNAs) [ 6 ]. (ijbs.com)
The first class of small RNAs identified were the micro RNAs (miRNAs), which are small (~22 nt) non-coding RNAs that regulate gene expression by base pairing to mRNAs where they direct either mRNA cleavage or repress translation [ 8 ]. (biomedcentral.com)
MicroRNAs (miRNAs) are small regulatory non-coding RNAs, resulting from the cleavage of long primary transcripts (pri-miRNAs) in the nucleus by the Microprocessor complex generating precursors (pre-miRNAs) that are then exported to the cytoplasm and processed into mature miRNAs. (mdpi.com)
Some miRNAs are hosted in pri-miRNAs annotated as long non-coding RNAs (lncRNAs) and defined as MIRHGs (for miRNA Host Genes). (mdpi.com)
A large number of miRNAs are encoded in organized intronic clusters within many protein coding genes. (biomedcentral.com)
Regulatory noncoding microRNAs (miRNAs) play a critical role in the regulation of gene expression [ 5 ] and are potential candidates for studying their role in the progression of PCa to androgen-independent stage. (biomedcentral.com)
Gene expression modulating miRNAs are encoded in diverse genomic locations including intergenic regions, introns of protein-coding genes and introns/exons of noncoding RNA genes [ 36 ]. (biomedcentral.com)
It is likely that the intronic miRNAs are processed from the same primary transcript as the precursor mRNAs and thus, their expression levels are regulated by the expression of the host mRNA [ 39 ]. (biomedcentral.com)
MiRNAs are short non-coding RNAs that play a central role in regulating RNA silencing and gene expression. (encyclopedia.pub)
MiRNAs are short non-coding RNAs (ncRNAs) that function in the regulation of RNA silencing and gene expression [ 2 ] . (encyclopedia.pub)
Like the expression of miRNAs, alternative splicing events are also rigorously regulated processes and aberrant splicing events give rise to malignancy and resistance to therapy [ 6 ] . (encyclopedia.pub)
Evidence suggests that there is an interplay between miRNAs and alternative splicing events that alters drug resistance [ 1 ] . (encyclopedia.pub)

Here, we highlight that an important RNA sequence region, encompassing an exon-intron hairpin loop (also called IDX-rasISS1), of the H-Ras pre-mRNA may encode an ncRNA that regulates p68 RNA helicase. (lidsen.com)
Thirty Years with ERH: An mRNA Splicing and Mitosis Factor Only or Rather a Novel Genome Integrity Protector? (xenbase.org)
Structures of mammalian GLD-2 proteins reveal molecular basis of their functional diversity in mRNA and microRNA processing. (xenbase.org)
microRNA-mRNA interactions occur in nucleus and cytoplasm of gastric cancer cells. (biomedcentral.com)
Additionally, circRNAs modulate pre-mRNA alternative splicing and possess protein-coding capacity. (ijbs.com)
However, circRNAs were initially regarded as viral genomes or byproducts of pre-mRNA alternative splicing, and thus they did not gain much attention for a long time [ 15 ]. (ijbs.com)
Polyadenylation is essential for the stability of the transcript, preventing the mRNA from being the target of posttranscriptional gene silencing (PTGS) via RNA-dependent RNA polymerase 6 (RDR6) in plants ( Luo and Chen, 2007 ). (frontiersin.org)
Studies in rat found that this enzyme acted on its own pre-mRNA molecules to convert an AA dinucleotide to an AI dinucleotide which resulted in a new splice site. (cancerindex.org)
When the gene is transcribed, the exon RNA is spliced into mRNA, and most of the intronic RNA is discarded. (opensourcebiology.eu)
In bacteria , a single RNA polymerase produces the primary transcript precursors for all three major classes of RNA: messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA). (basicmedicalkey.com)
During splicing reactions , introns are removed and the exons connected to form the mature mRNA. (basicmedicalkey.com)
For some genes, the abundance of the circular RNA exceeds that of the associated linear mRNA by a factor of 10, raising the interesting possibility that the function of some protein-coding genes may actually be to produce circular noncoding RNAs, not proteins. (bcm.edu)
In total, these results indicate that inhibition or slowing of canonical pre-mRNA processing events shifts the steady-state output of protein-coding genes towards circular RNAs, which likely helps explain why and how circular RNAs show tissue-specific expression profiles. (bcm.edu)
In particular, we are interested in determining how cellular cues can alter the ratio of linear mRNA to circular RNA for a given gene. (bcm.edu)
Alternative splicing may change the target mRNA 3′UTR binding site. (encyclopedia.pub)
This warrants urgent attention to, and targeting of, molecular alterations, such as miRNA regulation and alternative mRNA splicing, which hold potent therapeutic potential. (encyclopedia.pub)
Alternative splicing (AS) contributes to several cellular functions by maintaining the diversity of the proteome, producing several differently spliced mRNA from a single gene for translation [ 6 ] . (encyclopedia.pub)
LHCGR has 11 exons and codes for multiple alternatively spliced species (at least 6) of mRNA. (biomedcentral.com)
Its pre-mRNA is sex-specifically spliced and encodes female-specific or male-specific polypeptides. (bioone.org)
However, intron 4 is so long that it is difficult to identify the intronic cis-element(s) required for male-specific splicing of Bmdsx pre-mRNA using Bmdsx minigenes whose introns are shortened in various manners. (bioone.org)
Bmdsx pre-mRNA transcribed derived from transgene was sex-specifically spliced. (bioone.org)
These results reveal a central role of RNA structure dynamics in gene regulatory programs during embryogenesis.This is the developmental mRNA-seq timecourse part of the study. (refine.bio)
At the post-transcriptional level, lncRNAs can act as mRNA regulators to change either translation or mRNA stability, and they have been reported to modulate alternative splicing of mRNA. (enlightenbio.com)

Long noncoding RNAs (lncRNAs) are a class of endogenous, non-protein coding RNAs that are highly linked to various cellular functions and pathological process. (biomedcentral.com)
Long noncoding RNAs (lncRNAs) represent a vast unexplored genetic space that may hold missing drivers of tumourigenesis, but few such "driver lncRNAs" are known. (nature.com)
Amongst the most numerous, yet poorly understood of the latter are long noncoding RNAs (lncRNAs). (nature.com)
Noncoding RNAs (ncRNAs), such as microRNAs and long noncoding RNAs (lncRNAs), participate in cellular transformation. (oncotarget.com)
Long non-coding RNAs (lncRNAs) are clinically relevant in at least two major ways: as biomarkers for cancer or carcinogenesis and as actual targets for cancer therapy. (enlightenbio.com)
In other instances, lncRNAs can act as molecular decoys or guides for proteins that bind to DNA or other RNAs. (enlightenbio.com)

Circular RNAs (circRNAs) have been identified as competing endogenous RNAs (ceRNAs) to mediate gene expression participating in the progression of multiple cancers, including gastric carcinoma (GC). (archivesofmedicalscience.com)
In our study, hsa_circ_0017728 as a potential oncogene competing endogenous RNA (ceRNA) was investigated in the progression and development of gastric carcinogenesis. (archivesofmedicalscience.com)
CircRNAs are a novel class of endogenous noncoding RNA and are characterized by single-stranded, covalently closed circular molecules without 5′ to 3′ polarity and a polyadenylated tail [ 5 , 6 ]. (archivesofmedicalscience.com)
Circular RNAs (circRNAs) are a new type of endogenous noncoding RNA that exhibit a variety of biological functions. (biomedcentral.com)

Exosomes are extracellular vesicles with a lipid bilayer membrane that can be released by all types of cells, which contain a variety of proteins, lipids, and a variety of non-coding RNAs. (ijbs.com)
In addition, after transcription, a wide array of RNA-binding proteins interacts with cis -acting elements located mainly in the 3' untranslated region, determining the fate of mRNAs in eukaryotic cells. (frontiersin.org)
But they can all selectively bind to targets, such as messenger RNA transcripts, to promote or inhibit their translation into proteins. (opensourcebiology.eu)
NEAT1 scaffolds RNA-binding proteins and the Microprocessor to globally enhance pri-miRNA processing. (ablife.cc)
We identified trans-factors by profiling RNA-protein interactions and found that poly-U binding proteins are preferentially associated with 3'-UTR sequences and stabilizing motifs. (refine.bio)
2012) that up to 75% of the human genome is transcribed into processed RNA transcripts even though less than 2% of the genome encodes proteins. (enlightenbio.com)

CircRNAs are predominantly generated in eukaryotes via four cyclized models: back-spliced exons, circular intronic RNAs, exon-intron circRNAs and intergenic circRNAs [ 7 , 8 ]. (archivesofmedicalscience.com)
Circular RNAs (circRNAs), a novel member of the lncRNA kingdom, are generated by non-sequential backsplicing of exons, introns or a combination of both [ 7 - 9 ]. (ijbs.com)
But some intronic RNA can become small RNA Involved in Protein production Why eukaryotes have introns is an open question, but researchers suspect that introns help accelerate genetic evolution by making it easier for exons to recombine into new combinations. (opensourcebiology.eu)
With the exception of the first and last exons of genes, every other exon in the genome has splicing signals at its 5' and 3' ends and theoretically can circularize. (bcm.edu)
However, every exon does not circularize, and, in some cases, multiple exons are present in a circular RNA. (bcm.edu)

Circular RNAs (circRNAs) are a type of RNAs that are more abundant, specific, and highly organized, compared to other types of RNAs. (ijbs.com)
Circular RNAs (circRNAs) are noncoding RNAs with widespread distribution and diverse cellular functions. (biomedcentral.com)
CircRNAs have essential functions as microRNA sponges, RNA-binding protein- and transcriptional regulators, and protein translation templates. (biomedcentral.com)
Because of this extraordinary construction, circRNAs are resistant to degradation by exonucleases and are more stable than linear RNA. (biomedcentral.com)
Circular RNAs (circRNAs), a novel type of non-coding RNAs (ncRNAs), are ubiquitously expressed in eukaryotic cells during post-transcriptional processes. (ijbs.com)
Unlike linear RNAs, circRNAs form covalent-closed continuous loops without 5' to 3' polarities and poly (A) tails. (ijbs.com)
More importantly, circRNAs serve as microRNA (miRNA) sponges and crucial regulators of gene expression. (ijbs.com)
CircRNAs were first identified in RNA viruses in the 1970s [ 12 - 14 ]. (ijbs.com)
We found 1100 differentially expressed circRNAs (675 downregulated and 425 upregulated) which were involved in various biological processes such as protein ubiquitination modification, cell cycle regulation, RNA transport, and autophagy. (biomedcentral.com)
RNA-Seq results were validated by circRNAs qRT-PCR. (biomedcentral.com)
Circular RNAs (circRNAs) belong to a class of non‐coding RNAs that are widespread in the cytoplasm of eukaryotic cells and are structurally and functionally different from linear RNA molecules [ 1 ]. (biomedcentral.com)
Herein, we wondered whether circular RNAs (circRNAs), a type of RNA transcripts lacking 5′-3′ ends and forming closed loops that are gaining relevance in cancer biology, are also a target of epigenetic inactivation in tumors. (oncotarget.com)

These are long RNA transcripts that share many characteristics of mRNAs, with the key difference that they do not contain any recognizable Open Reading Frame (ORF), and thus are unlikely to encode protein 4 . (nature.com)
The primary transcripts are modified and trimmed to produce the mature RNAs. (basicmedicalkey.com)
Upon inhibiting RNA polymerase II termination, circular RNA levels were similarly increased due to readthrough transcripts extending into downstream genes and being subjected to backsplicing. (bcm.edu)
Approximately just 2% of the genome is transcribed into protein-coding RNAs [ 1 ], so the majority of transcripts are noncoding RNAs (ncRNAs), that can be categorized according to their structural properties and length [ 2 ]. (oncotarget.com)
The candidate interval was shown to exhibit an extraordinary level of alternate splicing - 19 transcripts were identified for the 5 protein coding genes, but it appears that a significant portion (14/19) of these alternate transcripts did not have an open reading frame, hence their functional role is questionable. (biomedcentral.com)
These transcripts may result from aberrant rather than regulated splicing. (biomedcentral.com)

Furthermore, RNAi assays containing the hairpin loop as an miRNA precursor were conducted, using the pTer vector, to explore the effects on 68 RNA helicase expression levels. (lidsen.com)
The use of microRNA (miRNA) manipulation is one approach which has been fruitful in boosting cell specific productivity but at a cost of cellular growth rate, ultimately diminishing volumetric titre. (dcu.ie)
This study aims to reveal the mechanisms of miRNA-RNA interactions in nucleus and cytoplasm. (biomedcentral.com)
Then, the Dicer protein can act on the pre-miRNA and cleave the end loop of the pre-miRNA to generate a double-stranded RNA with a length of approximately 22 base pairs (bp) [ 1 , 2 ]. (biomedcentral.com)
The characterization of new miRNA target genes and a unique profile for crasiRNAs has allowed for insight into multiple RNA mediated processes in the tammar, including gene regulation, species incompatibilities, centromere and chromosome function. (biomedcentral.com)
More importantly, our data indicate the possibility of additional novel mechanisms for intronic miRNA processing in PCa cells. (biomedcentral.com)
Marima, R. Normal and Aberrant MiRNA and Alternative Splicing Events. (encyclopedia.pub)
Normal and Aberrant MiRNA and Alternative Splicing Events" Encyclopedia , https://encyclopedia.pub/entry/16911 (accessed December 01, 2023). (encyclopedia.pub)
MiRNA expression and alternative splicing events are rigorously regulated processes. (encyclopedia.pub)
Dysregulation of miRNA and splicing events promote carcinogenesis and drug resistance in cancers including breast, cervical, prostate, colorectal, ovarian and leukemia. (encyclopedia.pub)
Molecular events such as alterations in microRNA (miRNA) transcription and aberrant splicing events contribute to drug resistance in many cancers [ 1 ] . (encyclopedia.pub)

These are ca. 21 nt regulatory RNAs that control development and differentiation acting as post-transcriptional negative regulators of the expression of key target genes. (unitn.it)
Small RNAs play important roles in many aspects of pre- and post-transcriptional gene regulation, epigenetic modifications, chromosome segregation and genome structure. (biomedcentral.com)

These results indicate that RNA splicing events occur in platelets during activation and that maturation of specific pre-mRNAs is part of the activation cascade, contributing to a dynamic fine-tuning of the transcriptome. (nature.com)
Finally, alternative splicing microarrays containing apoptosis targets were incubated to verify whether pre-mRNAs other than H-Ras could also present a similar hairpin loop structure regulated by p68 RNA helicase. (lidsen.com)
The double-stranded RNA duplex leads RNA-induced silencing complex (RISC), containing a member of the conserved Argonaute (Ago) protein family, to target sites of mRNAs, resulting in the destabilization of the mRNAs and/or inhibition of translation [ 4 , 5 ]. (biomedcentral.com)
Surprisingly, when spliceosome components were depleted or inhibited pharmacologically, the steady-state levels of circular RNAs increased while expression of their associated linear mRNAs concomitantly decreased. (bcm.edu)
Alternative splicing increases the diversity of the proteome by producing several different spliced mRNAs from a single gene for translation. (encyclopedia.pub)

In silico analysis revealed the presence of resident immature RNAs in resting platelets, characterized by retained introns, while unbiased proteogenomics correlated intron removal by RNA splicing with changes on proteome composition upon activation. (nature.com)
When repeat sequences from the flanking introns base pair to one another, the splice sites are brought into close proximity and backsplicing occurs. (bcm.edu)

The importance of alternative splicing for the diversity of the proteome and the large number of genetic diseases that are due to splicing defects call for methods to modulate alternative splicing decisions. (unitn.it)

In efforts to re-engineer synthetic sponge-decoys we encountered circular RNAs (circRNA). (dcu.ie)
The potential of circular transgene open reading frames (ORF) as a means of improving translational output from an RNA molecule was investigated using a model recombinant glycoprotein, Erythropoietin (EPO). (dcu.ie)
Wang M, Yu F, Wu W, Zhang Y, Chang W, Ponnusamy M, Wang K, Li P. Circular RNAs: A novel type of non-coding RNA and their potential implications in antiviral immunity. (ijbs.com)
Deep sequencing has revealed thousands of eukaryotic protein-coding genes that defy the central dogma, producing circular noncoding RNAs rather than linear messenger RNAs. (bcm.edu)
Once produced, it is largely unclear what circular RNAs do, although two are known to efficiently modulate the activity of microRNAs. (bcm.edu)
Backsplicing produces circular RNAs From Wilusz and Sharp (2013) Science 340: 440-441. (bcm.edu)
Although mechanistically simple, this step occurs in a highly selective manner, as the sequence of the repeats can drastically alter the efficiency of circular RNA production. (bcm.edu)
How then is the ratio of linear to circular RNA controlled or modulated? (bcm.edu)
We propose that this is because cross-exon interactions are not easily replaced with cross-intron interactions, thereby causing spliceosomes to preferentially assemble across an exon and generate a circular RNA. (bcm.edu)
Most mature circular RNAs accumulate in the cytoplasm, and we revealed the first insights into how their nuclear export is controlled in a length-dependent manner. (bcm.edu)
We further developed improved methods for circular RNA identification and purification using RNase R by identifying a way to better remove linear RNAs containing G-quadruplexes or structured 3' ends. (bcm.edu)
By revealing the fundamental mechanisms by which circular RNAs are generated, we have developed plasmid and viral-based methods for ectopically expressing circular RNAs. (bcm.edu)
Almost any sequence can now be efficiently circularized in eukaryotic cells, which has enabled us (and others) to begin to ask how circular RNAs function. (bcm.edu)
More than 150 labs have requested our circular RNA expression plasmids, highlighting our wide impact on this growing field. (bcm.edu)
We are continuing to elucidate the mechanism by which circular RNAs are produced. (bcm.edu)

An intronic insertion in KPL2 results in aberrant splicing and causes the immotile short-tail sperm defect in the pig. (au.dk)
Recent data show aberrant and altered expression of regulatory noncoding micro (mi) RNAs in prostate cancer (PCa). (biomedcentral.com)

MicroRNAs Regulate TASK-1 and Are Linked to Myocardial Dilatation in Atrial Fibrillation. (xenbase.org)
Cells use some of their non-coding DNA to create various RNA molecules that regulate or assist protein production in various ways. (opensourcebiology.eu)

Genes are transcribed by enzymes called RNA polymerases that generate a single-stranded RNA identical in sequence (with the exception of U in place of T) to one of the strands of the double-stranded DNA. (basicmedicalkey.com)

Eukaryotic genes are transcribed in the nucleus by three different RNA polymerases , each principally responsible for one of the major classes of RNA. (basicmedicalkey.com)
The upregulated DEGs were primarily protein‑coding genes enriched in immune and inflammation response pathways, while the downregulated DEGs were mainly coding long non‑coding (lnc)RNAs, indicating the regulatory function of USF1. (spandidos-publications.com)
Alternative splicing is known to take place in many genes that can promote or inhibit drug resistance. (encyclopedia.pub)

Non-coding RNAs (ncRNAs) have emerged as one of the most abundant regulatory molecules. (lidsen.com)
It has become progressively evident that RNA is not just a carrier of genetic information, but also a catalyst and a guide for sequence-specific recognition and processing of other RNA molecules. (unitn.it)
Progress in our understanding of RNA biology has made it possible to identify RNA molecules as targets of therapeutic intervention and to use RNA as a tool for functional studies and as a novel therapeutic molecule to treat human disease.Since a few years, RNA-based techniques (RNA interference, antisense RNA etc.) are becoming more and more useful in therapy and applied research. (unitn.it)
They are covalently closed-loop RNA molecules that are formed by back-splicing of the 5′ and 3′ ends of the primary transcript. (biomedcentral.com)
Small RNAs have proven to be essential regulatory molecules encoded within eukaryotic genomes. (biomedcentral.com)

Integrator endonuclease drives promoter-proximal termination at all RNA polymerase II-transcribed loci. (rochester.edu)
Specific sequences on DNA, called promoters , determine where the RNA polymerase binds and how frequently it initiates transcription. (basicmedicalkey.com)

Although splicing can be modulated by antisense oligonucleotides, this approach is confronted with problems of efficient delivery and the need for repeated administrations of large amounts of oligonucleotides. (unitn.it)

Artificial circularization of gene ORF was achieved by splice signals, made proximal via complementary intronic flanking sequence. (dcu.ie)
The DNA strand that directs the sequence of nucleotides in the RNA by complementary base pairing is the template strand. (basicmedicalkey.com)

However, it has been proven that more than 62% of genomic DNA serves as a template for transcription, which indicates that there are abundant non-coding RNAs (ncRNAs) in human transcriptome [ 2 , 3 ]. (ijbs.com)
Huh7 cells that overexpress USF1 were used with whole transcriptome profiling through RNA sequencing and chromatin immunoprecipitation (ChIP) sequencing methods to investigate the downstream targets of USF1. (spandidos-publications.com)
However, our knowledge of the global structure of the transcriptome is limited to steady-state conditions, hindering our understanding of how RNA structure dynamics influences gene function. (refine.bio)

Therefore, we developed a method to induce the efficient and specific skipping of an exon with the help of the U1 small nuclear RNA involved in pre-messenger RNA splicing in the nucleus. (unitn.it)

A gene is a segment of DNA that functions as a unit to generate an RNA product or, through the processes of transcription and translation, a polypeptide chain. (basicmedicalkey.com)
e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens. (lookformedical.com)

These short RNAs participate in a diverse array of cellular processes including gene regulation, chromatin dynamics and genome defense. (biomedcentral.com)
The RNA-stabilizing properties of RNAprotect Tissue Reagent prevent gene induction or down-regulation triggered by sample manipulation allowing you to preserve and analyze the gene expression profile. (qiagen.com)
This result shows that the mini gene contained the information necessary for the correct regulation of alternative splicing. (bioone.org)

To validate our hypothesis , we directly compared p68 RNA sequences and the hairpin loop in silico . (lidsen.com)
Thus it can be inferred from our findings that the alternative splicing exon IDX from H-Ras, coupled with the immediately downstream intron sequences, may contain an ncRNA. (lidsen.com)
These noncoding regions contain a wealth of regulatory sequences and non-coding RNAs whose role in cancer has been neglected until now 3 . (nature.com)
We examined each of these small RNA classes with respect to the newly assembled tammar wallaby genome for gene and repeat features, salient features that define their canonical sequences, and the constitution of both highly conserved and species-specific members. (biomedcentral.com)
Intronic sequences are shown in lower case. (xenbase.org)

Alternative polyadenylation alters protein dosage by switching between intronic and 3'UTR sites. (rochester.edu)
PolyAMiner-Bulk: A Machine Learning Based Bioinformatics Algorithm to Infer and Decode Alternative Polyadenylation Dynamics from bulk RNA-seq data. (rochester.edu)

A survey of the tammar gonadal piRNAs shows that these small RNAs are enriched in retroelements and carry members from both marsupial and tammar-specific repeat classes. (biomedcentral.com)
Another class of important small RNAs is the piRNAs. (biomedcentral.com)

The changes in alternative splicing and the resulting change in protein expression may affect the effectiveness of various treatments by altering drug uptake and efflux, altering the target of the drug, altering the conversion of the drug to its active state or metabolites, increased drug sequestration, drug inactivation, altered apoptosis, altered DNA damage response (DDR), altered cellular communication and immune system evasion [ 7 ] . (encyclopedia.pub)

Finally, we also identified a similar hairpin loop-like structure in an alternative splicing region of MAPK12/ERK6. (lidsen.com)
We also unveil one potential function of this ncRNA whose expression is regulated by alternative splicing decisions. (lidsen.com)
The 10-amino-acid GßÎ³-interacting domain in TRPM3 is subject to alternative splicing. (bvsalud.org)
Alternative splicing of this gene results in several transcript variants, some of which have been characterized by the presence or absence of an ALU cassette insert and a short or long C-terminal region. (cancerindex.org)
Cancer cells are known to make use of alternative splicing to alter their sensitivity to chemo-, radio-, hormonal, and immunotherapies by changing the expression profile of various protein isoforms. (encyclopedia.pub)

This study encompasses the first analyses of the major classes of small RNAs for the newly completed tammar genome, validates preliminary annotations using deep sequencing and computational approaches, and provides a foundation for future work on tammar-specific as well as conserved, but previously unknown small RNA progenitors and targets identified herein. (biomedcentral.com)

As reported, miR-25 and miR-92a show clear nuclear enrichment in rat primary cortical neurons by using a combination of microarray analysis and small RNA deep sequencing [ 10 ]. (biomedcentral.com)

As part of the genome sequencing initiative for the tammar, we have explored the evolution of each of the major classes of mammalian small RNAs in an Australian marsupial for the first time, including the first genome-scale analysis of the newest class of small RNAs, centromere repeat associated short interacting RNAs (crasiRNAs). (biomedcentral.com)

Targeted Transcriptomics of Frog Virus 3 in Infected Frog Tissues Reveal Non-Coding Regulatory Elements and microRNAs in the Ranaviral Genome and Their Potential Interaction with Host Immune Response. (xenbase.org)
Furthermore, we find that dynamic 3'-UTR structures are enriched in RNA decay elements, including regulatory elements in nanog, and cyclin A1, key maternal factors orchestrating the maternal-to-zygotic transition. (refine.bio)

Pro- and anti-apoptotic microRNAs are differentially regulated during estivation in Xenopus laevis. (xenbase.org)

Costello, Alan (2019) RNA manipulations to improve recombinant protein production in Chinese hamster ovary cells. (dcu.ie)

These RNAi analyses were quantified by Western blots (using anti-p68 RNA helicase and anti-EIF 2 α ) and Fluc/Rluc 3'UTRs/CDS assays. (lidsen.com)

her research on RNA Therapies for neurodegenerative disease in an interview by Osservatorio Terapie Avanzate (in italian). (unitn.it)
Professor Denti spoke about RNA Therapies in a video interview on RAI 3 (in italian). (unitn.it)

RNA polymerases differ from DNA polymerases in that they can initiate the synthesis of new strands in the absence of a primer. (basicmedicalkey.com)
The transcribed region of a gene contains the template for synthesis of an RNA, which begins at the start point. (basicmedicalkey.com)

Moreover, a similar effect on p68 RNA helicase-mediated interference is observed during the upregulation of the hairpin loop. (lidsen.com)

Anatomy1

Organisms1

Chemicals and Drugs28

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes21

Information Science3

MiRNAs23

MRNA23

LncRNAs6

Endogenous4

Proteins6

Exons5

CircRNAs12

Transcripts6

MiRNA11

Post-transcriptional2

MRNAs5

Introns2

Diversity of the proteome1

Circular15

Aberrant2

Regulate2

Single-stranded1

Genes3

Molecules5

Polymerase2

Antisense oligonucleotides1

Sequence2

Transcriptome3

Small nuc1

Transcription2

Regulation3

Sequences5

Polyadenylation2

PiRNAs2

Cellular1

Alternative5

Targets1

Microarray1

Mammalian1

Regulatory Elements2

Differentially1

20191

RNAi1

Therapies2

Synthesis2

Interference1