Spliceosome mediated rna. Medical search. Frequent questions

Both proteins mediate protein-protein and protein-RNA interactions for cooperative RNA-binding during spliceosome assembly. (cipsm.de)
RNA binding proteins (RBPs) regulate the diverse steps in RNA processing, including alternative splicing, which generates fiber-type specific isoforms of structural proteins that confer contractile sarcomeres with distinct biomechanical properties. (uni-muenchen.de)
By integrating multiple RNA-Seq datasets from siRNA-mediated perturbations of core components of the spliceosome, we have defined groups of spliceosomal proteins implicated in specific changes in alternative splice site choice. (fu-berlin.de)
This enhancement of RNA splicing by ORF57 correlates with the intact N-terminal nuclear localization signal motifs of ORF57 and takes place in the absence of other viral proteins. (pasteur.fr)
The above reactions are mediated by a large RNA-protein complex, the spliceosome, which consists of five types of snRNA (small nuclear RNA) and more than 200 proteins [ 2 ]. (ijbs.com)
Among these, three U4/U6·U5 snRNP proteins (hBrr2, hPrp4, and hPrp31) copurified with CBC in an RNA-independent fashion, suggesting that a significant fraction of CBC forms a complex with the U4/U6·U5 snRNP and that the activity of CBC might be associated with snRNP recruitment to pre-mRNA. (iucc.ac.il)
This process is mediated by a complex molecular machinery known as the spliceosome, which enables the production of multiple and functionally distinct proteins from single genes. (lu.se)
In this study, Dr Joshua Shulman and his colleagues investigated a molecular mechanism called RNA splicing that is involved in the production of mature RNA molecules necessary to produce working proteins. (drugtargetreview.com)
Since many spliceosomal proteins were present in low levels in the neurons of flies over-expressing toxic tau, the researchers proposed that tau aggregates either disrupted the proper assembly of the spliceosome or sequestered key components in the cytoplasm, away from the site of action in the nucleus. (drugtargetreview.com)
RNA alternative splicing (AS) is an important post-transcriptional mechanism enabling single genes to produce multiple proteins. (nature.com)
The spliceosome complex, composed of at least 170 proteins and several small nuclear RNAs (snRNAs), is the key structure responsible for splicing in eukaryotes 10 . (nature.com)
This process is carried out by the human spliceosome machinery, in which over 300 proteins sequentially assemble with uridine-rich small nuclear RNA molecules (U snRNAs) to form distinct small nuclear ribonucleoprotein complexes (snRNPs). (biomedcentral.com)
Coding sequences in eukaryotic genomes are frequently interrupted by spliceosomal introns, regions of noncoding DNA that are removed from pre-mRNA transcripts by the spliceosome, a complex of five RNAs and hundreds of proteins [ 1 ]. (biomedcentral.com)
Multiple gene mutations have been identified and considered as important substrates for the development of MDS, such as RNA splicing, histone manipulation, DNA methylation, transcription factors, kinase signaling, DNA repair, cohesin proteins, and other signal transduction elements. (frontiersin.org)
The high sequence conservation of HSPC117/RtcB proteins is suggestive of RNA ligase roles of this protein family in various organisms. (nih.gov)
Family of C2H2-type zinc fingers, present in matrin, U1 small nuclear ribonucleoprotein C and other RNA-binding proteins. (embl.de)
This process is carried out by a megadalton complex called the spliceosome that consists of more than 200 proteins and snRNA molecules. (wustl.edu)
Previous studies have shown that alterations to a DNA sequence of spliceosome proteins introduce errors in the splicing process that leads to incorrect splicing. (wustl.edu)
Many spliceosome proteins have been implicated in diseases like neurodegenerative disorders, retinitis pigmentosa, cancer and spinal muscular atrophy. (wustl.edu)
Thus, it is important to understand the role of individual spliceosome proteins in the splicing process and their effect on splicing fidelity. (wustl.edu)
A subset of spliceosome proteins forms the core component of the spliceosome that are present throughout the splicing process, while other proteins are transient that bind and leave the complex at different points during the splicing process. (wustl.edu)
Our lab previously characterized loss-of-function mutations in four different spliceosome proteins that act as suppressors of splice site mutations in Chlamydomonas reinhardtii. (wustl.edu)
Interestingly, three out of four proteins are part of the small group of proteins that joins the catalytically active spliceosome complex C late in the splicing process. (wustl.edu)
This dissertation focuses on understanding the role of two of these spliceosome proteins, DGCR14 and FRA10AC1. (wustl.edu)
Further work to investigate the interaction of these factors with other spliceosome proteins and 5' and 3' splice site is warranted. (wustl.edu)
Together my research work in this dissertation reveals new insights into the post-transcriptional regulation of genes by alternative splicing in C. reinhardtii and highlights the role of non-core spliceosome proteins in maintaining the splicing fidelity and selection of weak splice sites. (wustl.edu)
Non-coding RNAs can regulate gene expression by affecting the localization and function of epigenetic modifier proteins, including histone deacetylases. (mchughlab.com)
The most well-studied example of a functional non-coding RNA is the X inactive specific transcript (XIST) which controls the function of SHARP and HDAC proteins to silence an entire X chromosome during female embryonic development. (mchughlab.com)
Through structural studies and mutational analyses, we aim to understand how long non-coding RNAs can localize and organize proteins in the cell in time and space. (mchughlab.com)
This study is to explore the biogenesis mechanism of a secondary hepatocellular carcinoma (HCC) based on the function of RNA binding proteins (RBPs)-encoding genes in the physiological microenvironment (PM). (peerj.com)
This is done by a complex of proteins and RNAs called spliceosome. (stemcelldaily.com)
Each RNA polymerase requires the assistance of several other proteins or protein complexes, called general (or basal) transcription factors, which must assemble into a complex on the promoter in order for RNA polymerase to bind and start transcription. (stemcelldaily.com)
Further characterization showed that susa2-2 only suppresses the autoimmunity mediated by either CHS1-SOC3 or TN2-SOC3 paired NLR proteins, indicating that SUSA2 is specifically involved in NLR protein SOC3-mediated immunity. (ubc.ca)

Alternative splicing does not only lead to differential isoform expression, but can, through the inclusion of premature termination codons, also induce nonsense-mediated decay (NMD) to control the overall abundance of mRNAs. (fu-berlin.de)
in addition to roles in miRNA biogenesis, nonsense-mediated decay, 3'- end formation, and snRNA export from the nucleus, CBC promotes pre-mRNA splicing. (iucc.ac.il)
I analyzed the splicing patterns in dgr14 and fra10 mutants in a wild-type background and in double mutants with a mutation that affects nonsense mediated decay (NMD) to capture the breadth of global splicing changes incurred by the spliceosome mutants. (wustl.edu)

Cotransfection of ORF57 and K8beta cDNA, which retains a suboptimal intron of K8 pre-mRNA due to alternative splicing, promoted RNA splicing of K8beta and production of K8alpha (K-bZIP). (pasteur.fr)
We aim to assess the possibility of LMNA-mRNA repair by spliceosome-mediated RNA trans-splicing (SMarT) as a potential therapeutic approach for L-CMD. (biomedcentral.com)
Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries (PubMed:21536736). (univ-amu.fr)
Stepwise sequential assembly of spliceosome components on these pre-mRNA motifs executes splicing reactions. (biomedcentral.com)
A recent report reveals widespread mis-splicing of RNA transcripts in eukaryotes, with mis-spliced RNA destroyed by nonsense-mediated mRNA decay. (biomedcentral.com)
Translation of RNAs carrying premature stop codons can be prevented by the nonsense-mediated mRNA decay pathway (NMD) [ 3 ], and Jaillon et al . (biomedcentral.com)
The immediate product of RNA polymerase II is sometimes referred to as pre-mRNA or the primary transcript . (pressbooks.pub)
Transcription is the process of copying genetic information from DNA into RNA, especially mRNA, by the enzyme RNA polymerase. (stemcelldaily.com)
It is the first step of gene expression, in which a particular segment of DNA is copied into RNA (especially mRNA) by the enzyme RNA polymerase. (stemcelldaily.com)
This releases the upstream portion of the transcript, which will serve as the initial RNA prior to further processing (the pre-mRNA in the case of protein-encoding genes). (stemcelldaily.com)
An upregulation of messenger RNA (mRNA) for IL-8 occurs in the inflammatory infiltrate near the border between necrotic and viable myocardium. (medscape.com)
RNA N6-methyladenosine reader IGF2BP3 promotes acute myeloid leukemia progression by controlling stabilization of EPOR mRNA. (cdc.gov)

It is usually found in eukaryotes and mediated by the spliceosome, although some bacteria and archaea also have "half-genes" for tRNAs. (wikipedia.org)
These early-diverging eukaryotes use few introns, and the spliceosome they possess show some unusual variations in their structure assembly. (wikipedia.org)
Spliceosome-mediated splicing IS a unique feature of eukaryotes. (pressbooks.pub)
Transcription in eukaryotes is the elaborate process that eukaryotic cells use to copy genetic information stored in DNA into units of transportable complementary RNA replica. (stemcelldaily.com)
Eukaryotes have three types of RNA polymerases (I, II and III), each responsible for transcribing different types of RNA. (stemcelldaily.com)

The nuclear cap-binding complex (CBC) binds to the 7-methyl guanosine cap present on every RNA polymerase II transcript. (iucc.ac.il)
Other transcription factors and RNA polymerase then assemble on the promoter to form a pre-initiation complex (PIC). (stemcelldaily.com)
Elongation involves the synthesis of RNA by RNA polymerase along the DNA template. (stemcelldaily.com)
The RNA polymerase transcribes one strand, the antisense (-) strand, of the DNA template. (stemcelldaily.com)
RNA synthesis occurs in the 5' → 3' direction with the RNA polymerase catalyzing a nucleophilic attack by the 3-OH of the growing RNA chain on the alpha-phosphorus atom on an incoming ribonucleoside 5-triphosphate. (stemcelldaily.com)
Termination occurs when RNA polymerase reaches a termination signal on the DNA template and releases the RNA transcript. (stemcelldaily.com)
Most promoters for RNA polymerase II also have a conserved sequence called the TATA box, which is recognized by a subunit of the transcription factor TFIID. (stemcelldaily.com)
The transcript is cleaved at an internal site before RNA polymerase II finishes transcribing. (stemcelldaily.com)

Trans-splicing is a special form of RNA processing where exons from two different primary RNA transcripts are joined end to end and ligated. (wikipedia.org)
We developed 5'-RNA pre-trans-splicing molecules (PTM) capable of repairing the murine LMNA transcripts. (biomedcentral.com)
Sequential assembly of the human spliceosome on RNA transcripts regulates splicing across the human transcriptome. (biomedcentral.com)
In humans, non-coding RNA transcripts are involved in many critical events in the life cycle of a cell, from gene expression regulation to protein synthesis by the ribosome. (mchughlab.com)
3) Uncovering new functions of cancer cell growth associated non-coding RNA transcripts. (mchughlab.com)

The team began by investigating how the levels of non-mutated SF3B1, a core spliceosome component, contribute to the MDS disease. (lu.se)
The core spliceosome component PRPF8 is essential for spliceosome assembly through its participation in ribonucleoprotein (RNP) complexes for splice-site recognition, branch-point formation and catalysis. (biomedcentral.com)

Small non-protein-coding RNA molecules, composed of around 22 nucleotides, are commonly named as miRNAs ( 1 - 3 ). (frontiersin.org)
To carry out RNA splicing, cells use the spliceosomal complex, a multiprotein cellular machinery that coordinates the production of mature RNA molecules. (drugtargetreview.com)
Splicing of mammalian precursor transfer RNA (tRNA) molecules involves two enzymatic steps. (nih.gov)
Chapters 8 and 9 introduced you to different components of eukaryotic gene structure and RNA molecules transcribed. (pressbooks.pub)

Mutations in minor spliceosome components such as U12 snRNA (cerebellar ataxia) and U4atac snRNA (microcephalic osteodysplastic primordial dwarfism type 1 (MOPD1)) result in tissue-specific symptoms. (biomedcentral.com)
Since U4atac snRNA is ubiquitously expressed, mutation in this gene is expected to result in systemic inhibition of the minor spliceosome. (biomedcentral.com)

In these organisms, a capped splice leader RNA is transcribed, and simultaneously, genes are transcribed in long polycistrons. (wikipedia.org)
Cluster identity was assigned based on the top 10 marker genes of each cluster ( Table S2 ), followed by inspection of RNA in situ hybridization patterns. (stowers.org)
We built a set T of flanking regions of genes with 1-3 introns from 21st and 22nd chromosomes extracted from GenBank to define positions having conserved properties, namely hydrophobicity, that are potentially essential for recognition by spliceosome. (ijbs.com)
Given that the minor spliceosome is ubiquitously expressed, we hypothesized that these restricted phenotypes might be caused by the tissue-specific regulation of the minor spliceosome targets, i.e. minor intron-containing genes (MIGs). (biomedcentral.com)
In these minor intron-containing genes (MIGs), the majority of introns are usually spliced by the major spliceosome, with only one or two introns being spliced by the minor spliceosome. (biomedcentral.com)
However, it remains unclear what features of these genes dictate dynamic changes in their recognition and stepwise processing by spliceosome components to regulate splice site choice and splicing outcome. (biomedcentral.com)

Trans-splicing is characterized by the joining of two separate exons transcribed RNAs. (wikipedia.org)
Circular RNAs (circRNAs), a novel member of the lncRNA kingdom, are generated by non-sequential backsplicing of exons, introns or a combination of both [ 7 - 9 ]. (ijbs.com)
The splicing mechanism involves the following steps: cleavage at 5′ splice site, nucleolytic attack of the splice donor site at the invariant A of the branch site to form a lariat-shaped structure, cleavage at 3′ splice site, leading to a release of the intronic RNA as a lariat, and ligation of the exons. (ijbs.com)
This requires that the RNA have a specific secondary and tertiary structure, bringing the two exons close together while looping out the intron. (pressbooks.pub)

Circular RNAs (circRNAs), a novel type of non-coding RNAs (ncRNAs), are ubiquitously expressed in eukaryotic cells during post-transcriptional processes. (ijbs.com)
Some eukaryotic ribonucleoprotein (RNP) complexes including the spliceosome and the ribosome have been examined in great detail, uncovering critical structure-function relationships of non-coding RNAs. (mchughlab.com)
Eukaryotic RNA polymerases do not terminate transcription at a specific site but rather transcription can stop at varying distances downstream of the gene. (stemcelldaily.com)

U2 snRNP-dependent introns make up the majority of all introns and are excised by spliceosomes containing the U1, U2, U4, U5 and U6 snRNPs. (ijbs.com)
U12 snRNP-dependent introns are the minor class of introns and are excised by spliceosomes containing U11, U12, U4atac, U6atac and U5 snRNPs. (ijbs.com)
Chromatin immunoprecipitation and live-cell imaging assays revealed decreased cotranscriptional accumulation of U4/U6·U5 snRNPs on active transcription units, consistent with a requirement for CBC in cotranscriptional spliceosome assembly. (iucc.ac.il)
Surprisingly, recruitment of U1 and U2 snRNPs was also affected, indicating that RNA-mediated interactions between CBC and snRNPs contribute to splicing. (iucc.ac.il)
Taken together, the data support a model whereby CBC promotes premRNA splicing through a network of interactions with and among spliceosomal snRNPs during cotranscriptional spliceosome assembly. (iucc.ac.il)

Spliceosome-mediated RNA trans-splicing (SMaRT) has been used previously to reprogram mutant endogenous CFTR and factor VIII mRNAs in human epithelial cell and tissue models and knockout mice, respectively. (utmb.edu)
Whereas "normal" (cis-)splicing processes a single molecule, trans-splicing generates a single RNA transcript from multiple separate pre-mRNAs. (wikipedia.org)
Genic trans-splicing allows variability in RNA diversity and increases proteome complexity. (wikipedia.org)
We further show that splicing mediated by SF1 and Hrb87F is necessary for Z-disc stability and proper myofibril development, and strong knockdown of either gene results in impaired localization of kettin to the Z-disc. (uni-muenchen.de)
Drosophila is thus a powerful model to gain disease-relevant insight into cellular and molecular phenotypes observed when expression levels of splicing factors, spliceosome components and splicing dynamics are altered. (uni-muenchen.de)
To address the functionality of body temperature controlled alternative splicing and gene expression we have used extensive analyses of RNA-Seq datasets. (fu-berlin.de)
After careful validation of bioinformatics predictions using splicing-sensitive RT-PCRs we use bioinformatics-based mutations of cis-regulatory RNA sequences or structure-guided mutations of trans-acting factors for a detailed mechanistic analysis of alternative splice site selection. (fu-berlin.de)
Her Zebrafish research is multidisciplinary, incorporating perspectives in RNA splicing, Spliceosome, Neural crest, RNA-binding protein and Intron. (research.com)
RNA splicing is a major nexus of gene expression regulation, shaping cellular identity during development, frequently altered in human cancers. (lu.se)
A new study has shown the possibility of using RNA splicing as a potential molecular target for Alzheimer's disease. (drugtargetreview.com)
Led by researchers at Baylor College of Medicine and the Jan and Dan Duncan Neurological Research Institute at Texas Children's Hospital, both US, a new study has revealed a novel mechanistic link and therapeutic target between alterations in RNA splicing and tau-mediated neurodegeneration in Alzheimer's disease. (drugtargetreview.com)
They looked into the possibility that aggregates of tau protein within neurons, a key marker of Alzheimer's disease, interfered with RNA splicing. (drugtargetreview.com)
Our findings present an exciting new possibility of using RNA splicing as a potential molecular target for Alzheimer's disease and other tau-mediated neurodegenerative conditions. (drugtargetreview.com)
Transcriptome-wide RNA-seq studies revealed that ASAP and PSAP complexes regulate distinct alternative splicing events both in EJC-independent and EJC-dependent manner. (nature.com)
When applied to large-scale transcriptomics projects with diverse taxonomic sampling, our new method is expected to rapidly expand our knowledge on RNA splicing mechanisms for a wide range of viruses. (nature.com)
RNA splicing plays important roles in viral replication and virus-host interactions 38 . (nature.com)
Here we show that minor intron splicing and AS across minor introns is a highly organised process that might be regulated in coordination with the major spliceosome in a tissue-specific manner. (biomedcentral.com)
Regardless, mis-splicing of these few minor introns can have detrimental consequences, as highlighted by diseases caused by mutation in minor spliceosome components. (biomedcentral.com)
Here, we show that alterations in RNA splicing patterns across the human transcriptome that occur in conditions of restricted cellular PRPF8 abundance are defined by the altered splicing of introns with weak 5′ splice sites. (biomedcentral.com)
Competition for PRPF8 availability alters the transcription-coupled splicing of RNAs in which weak 5′ splice sites predominate, enabling diversification of human gene expression during biological processes like mitosis. (biomedcentral.com)
That means for those RNAs, splicing happens autonomously, with part of the RNA acting as an enzymatic catalyst for the process. (pressbooks.pub)
The spliceosome undergoes a series of transitions from a pre-catalytic state (complex A) to catalytically active state (complex C) during the splicing process. (wustl.edu)
This suggests that the two splicing factors affect the splicing fidelity even though they join the spliceosome complex at late stage, pointing towards a potential proof-reading mechanism in splicing. (wustl.edu)
To capture the extent to which alternative splicing is active and functional in C. reinhardtii, I analyzed the existing RNA-seq data from Zones et al. (wustl.edu)
Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). (cancer-genetics.org)
Splicing factor 3b is also a component of the minor U12-type spliceosome. (cancer-genetics.org)

The proper regulation of RNA processing is critical for muscle development and the fine-tuning of contractile ability among muscle fiber-types. (uni-muenchen.de)
We have provided a framework to further study the impact of the minor spliceosome and the regulation of MIG expression. (biomedcentral.com)
This study also demonstrates a potential AS mediated regulation of ODC1 gene which encodes for ornithine decarboxylase enzyme, during light to dark transition during the diurnal cell cycle. (wustl.edu)
Proper regulation of immune responses mediated by NLRs is important as over-activation results in growth defects, while under-activation leads to vulnerability to pathogens. (ubc.ca)
Ubiquitin proteasome system (UPS)-mediated NLR turnover plays a pivotal role in the fine-tuned regulation of immune responses. (ubc.ca)

Researchers at Lund University Faculty of Medicine have determined a novel mechanism linking the metabolism of ribonucleic acids, RNA, to the development of leukemia in myelodysplastic syndrome patients, MDS. (lu.se)

Spliceosome mutations are common in myelodysplastic syndromes (MDS) and acute myeloid leukaemia (AML), but the oncogenic changes due to these mutations have not been identified. (cancer-genetics.org)

The overall similarity in the predicted secondary structure between analogous U2 and U12-type snRNAs suggests that the spliceosome rearrangements during catalysis are conserved between the two spliceosomes [ 3 - 4 ]. (ijbs.com)

In this study, the researchers began by over-expressing toxic tau in fruit flies as a model to test whether spliceosome-tau interactions might cause neurodegeneration. (drugtargetreview.com)
WW domain-mediated interactions reveal a spliceosome-associated protein that binds a third class of proline-rich motif: the proline glycine and methionine-rich motif. (embl.de)
1) Discovering non-coding RNA-protein interactions affecting gene expression through chromatin state. (mchughlab.com)
An improved understanding of RNA-protein interactions can enable the development of treatments to regulate cancer progression, correct developmental disorders, and slow the aging process. (mchughlab.com)

The RNAs play important roles in the pathological and physiological tissues including cancer. (peerj.com)

In animals, the second step predominantly entails direct exon ligation by an elusive RNA ligase. (nih.gov)

However, it has been proven that more than 62% of genomic DNA serves as a template for transcription, which indicates that there are abundant non-coding RNAs (ncRNAs) in human transcriptome [ 2 , 3 ]. (ijbs.com)

iCLIP of spliceosome components reveals that PRPF8 depletion decreases RNP complex formation at most splice sites in exon-intron junctions throughout the genome. (biomedcentral.com)

Prefoldins negatively regulate cold acclimation in Arabidopsis thaliana by promoting nuclear proteasome-mediated HY5 degradation. (bu.edu)

Expression of IRAK4-L is mediated by mutant U2 small nuclear RNA auxiliary factor 1 (U2AF1) and is associated with oncogenic signalling in MDS and AML. (cancer-genetics.org)

Violin plots show distribution of expression levels for ATP-dependent RNA helicase DHX8 (SMED30020946) in cells (dots) of each of the 12 neoblast clusters. (stowers.org)
Expression of ATP-dependent RNA helicase DHX8 (SMED30020946) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells. (stowers.org)

Wang M, Yu F, Wu W, Zhang Y, Chang W, Ponnusamy M, Wang K, Li P. Circular RNAs: A novel type of non-coding RNA and their potential implications in antiviral immunity. (ijbs.com)

Purification and characterization of native spliceosomes suitable for three-dimensional structural analysis. (univ-amu.fr)

A recent study found that the delivery of circRNAs generated in vitro activates RIG-I-mediated innate immune responses and provides protection against viral infection. (ijbs.com)
RIG-I and MDA5 are two distinct sensors of viral double-stranded RNA (dsRNA) , a replication intermediate for RNA viruses [1-3]. (invivogen.com)

The reconstitution of a stable EJC core requires single-stranded RNA (ssRNA) and ATP (or its non-hydrolysable form, ADPNP), and in the absence of either, the complex is not formed 3 . (nature.com)
2006. RIG-I mediated antiviral responses to single-stranded RNA bearing 5'-phosphates. (invivogen.com)

It results in a complementary, antiparallel RNA strand called a primary transcript. (stemcelldaily.com)

The long-term goal of our research is to gain an improved understanding of the structures and functions of RNA-protein complexes, in order to develop new approaches to treat human diseases and aging processes. (mchughlab.com)
2) Structural analysis of non-coding RNA and protein complexes from human cells. (mchughlab.com)
Non-coding RNA and protein complexes can control gene expression, hormone receptor responses, and ribosomal function. (mchughlab.com)

RNA-Seq and downstream bioinformatics allow the transcriptome-wide quantification of splice site choices. (fu-berlin.de)
Based on bioinformatics, public databases and our in-house RNA-Seq database, we analyzed the clinical value and molecular mechanism of PPP2CA in HCC. (xiahepublishing.com)
Recent bioinformatics studies identified a set of cancer-associated non-coding RNAs whose expression is linked to metastasis and decreased long-term patient survival. (mchughlab.com)
The samples were analyzed using the technology of RNA-seq and bioinformatics. (peerj.com)

The large ~280-kDa U5 snRNP protein PRPF8 is central to the dynamics of spliceosome assembly [ 11 ]. (biomedcentral.com)

Data were analyzed from 2,545 patients with HCC and 1,993 controls without HCC indexed in The Cancer Genome Atlas database, the Gene Expression Omnibus database and our in-house RNA-Seq database. (xiahepublishing.com)

RNA interference-mediated depletion of HSPC117 inhibited maturation of intron-containing pre-tRNA both in vitro and in living cells. (nih.gov)

Knockdown phenotypes of spliceosome components, as highlighted by phenotypes for A-complex components SF1 and Hrb87F (hnRNPA1), revealed level- and temporal-dependent myofibril defects. (uni-muenchen.de)
Prefoldins contribute to maintaining the levels of the spliceosome LSM2-8 complex through Hsp90 in Arabidopsis. (bu.edu)
Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. (univ-amu.fr)

6/2013 School Award - Joerg Braun, a postdoctoral associate, was awarded the Otto Hahn Medal for his outstanding PhD work on the mechanisms of miRNA-mediated gene silencing from the Max Planck Institute. (umassmed.edu)

Therapeutics targeting cancer cell growth and development have mainly focused on protein transcription factors and protein-mediated signaling pathways. (mchughlab.com)

SncRNAs are less than 200 nt in length, consisting of microRNAs (miRNAs), Piwi-interacting RNAs (piRNAs), endogenous small interfering RNAs (endo-siRNAs or esiRNAs) and transcription initiation RNAs (tiRNAs) [ 6 ]. (ijbs.com)

The spliceosome recognizes conserved sequences at the exon-intron boundaries, namely the 5' splice site (5'ss) and the 3' splice site (3'ss). (ijbs.com)

However, increasing evidence indicates that loss of physiological APP functions mediated predominantly by neurotrophic APPsα produced in the non-amyloidogenic α-secretase pathway may contribute to AD pathogenesis. (springer.com)

In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation (PubMed:11435423). (univ-amu.fr)

A snc1-influencing plant E3 ligase reverse (SNIPER) genetic screen identified two snc1-suppressors, SNIPER1 and SNIPER2, both of which completely suppress snc1-mediated autoimmunity upon overexpression. (ubc.ca)

The positions given by our method, which may be important for recognition by spliceosome, were compared to the nucleotide consensus provided by a classical method, Pictogram . (ijbs.com)

Anatomy7

Organisms12

Diseases2

Chemicals and Drugs101

Analytical, Diagnostic and Therapeutic Techniques and Equipment28

Phenomena and Processes71

Disciplines and Occupations1

Information Science4

Health Care3

Proteins33

Nonsense-media3

MRNA12

Eukaryotes5

Polymerase8

Transcripts5

Core spliceosome component2

Molecules4

SnRNA2

Genes6

Exons4

Eukaryotic3

SnRNPs5

Splicing26

Regulation5

Metabolism1

Mutations1

SnRNAs1

Interactions4

Roles1

Ligation1

Human transcriptome1

Splice Sites1

Regulate1

Small nuclear1

Helicase2

Immunity1

Characterization1

Viral2

Single-stranded2

Transcript1

Complexes3

Bioinformatics4

Dynamics1

Gene expression1

Depletion1

Complex3

MiRNA1

Therapeutics1

PiRNAs1

Exon-intron boundaries1

Physiological1

Substrates1

Overexpression1

Nucleotide1