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  • fertility
  • In vitro follicle techniques provide a tool to model follicle development in order to investigate basic biology, and are further being developed as a technique to preserve fertility in the clinic 1-4 . (jove.com)
  • Madrid, Spain: Scientists believe that they are an important step nearer to success in creating an artificial egg from the combination of the nucleus of a somatic cell and an oocyte which has had its DNA-carrying nucleus removed, a conference of international fertility experts heard today (Tuesday 1 July). (innovations-report.com)
  • The resulting increased incidence of aneuploidy likely accounts for the observed sub-fertility of mice harboring Hdac2 −/− oocytes. (prolekare.cz)
  • 2 Females who wish to electively preserve their fertility because of career commitments or because they have not met a male partner during their most fertile years, have also begun to utilize oocyte cryopreservation. (jlgh.org)
  • The purpose of this Funding Opportunity Announcement (FOA) is to encourage applications from the scientific community to support outstanding research in the area of oocyte mitochondrial function in relation to fertility, aging, and mitochondrial disease transmission to offspring. (nih.gov)
  • ovary
  • Oocytes are manually defolliculated from donor ovary tissue, injected or treated in culture as desired, and then stimulated with progesterone to induce maturation. (jove.com)
  • Here, we evaluated the effects of ovary storage at 4 °C on the preservation of preantral follicles and oocytes retrieved from antral follicles using the domestic cat as model. (biomedcentral.com)
  • 7 ] found that oocytes from rare wild species could be matured and fertilized in vitro following ovary storage for as long as 36 h in cold (4 °C) phosphate buffer saline (PBS). (biomedcentral.com)
  • Zuckerman S. The number of oocytes in the mature ovary. (springer.com)
  • factors to regulate
  • However, it is unclear how gonadotropins modulate these factors to regulate oocyte maturation, especially by gap junctional communication (GJC). (jove.com)
  • The recognition of oocyte maturation arrest as a specific medical condition may contribute to the characterization of the yet poorly defined entity currently known as 'oocyte factor' (oocyte secretes its own factors to regulate microenvironment in close neighbourhood of surrounding cells). (fertilitypedia.org)
  • follicles
  • Isolated follicles can be used for a variety of experimental techniques, including in vitro development assays, live imaging, mRNA expression analysis and western blot analysis of proteins. (jove.com)
  • In the following step, those follicles with visible antral cavities were isolated, from which 48 oocyte-granulosa cell complexes were removed. (weebly.com)
  • 1. McLaughlin M , Albertini DF, Wallace WHB , Anderson R , Telfer E . Metaphase II oocytes from human unilaminar follicles grown in a multi-step culture system. (weebly.com)
  • infertility
  • Oocyte maturation arrest may be the cause of infertility in some couples previously classified as having unexplained infertility. (fertilitypedia.org)
  • As it is difficult to identify maturation arrest of oocytes unless and ART (assisted reproduction techniques) procedure is done, such women are subjected to extremes of financial, emotional and physical burden due to repeated failures of infertility treatment. (fertilitypedia.org)
  • Simultaneously, advances in assisted reproductive techniques have led to new possibilities for the prevention and treatment of infertility. (aappublications.org)
  • germ cell
  • Co-expression of cyp26a1 and the pluripotent germ cell stem cell marker pou5f1(oct4) in meiotically arrested oocytes was consistent with roles in mouse to promote germ cell survival and to prevent apoptosis, mechanisms that are central for tipping the sexual fate of gonads towards the female pathway in zebrafish. (jove.com)
  • genetic
  • Thus, these methods are widely applicable to the future phenotypic characterization of adult zebrafish kidney injury paradigms, which include but are not limited to, nephrotoxicant exposure regimes or genetic methods of targeted cell death such as the nitroreductase mediated cell ablation technique. (jove.com)
  • this allows one to combine genetic or pharmacologic perturbations with in vitro development to define the effects of such manipulations on the processes occurring during specific periods of development. (jove.com)
  • In domestic felids considerable progress has been made in the development of modern methodologies of ARTs including in vitro embryo production and banking of genetic resources. (biomedcentral.com)
  • This technique can be used to enable genetic fatherhood to some men who have no spermatozoa in the ejaculate (azoospermia) and in whom spermatozoa cannot be obtained surgically from the testicles. (wikipedia.org)
  • The cellular and genetic mechanisms causing oocyte maturation arrest should be the subject of further investigation. (fertilitypedia.org)
  • Spindle replacement, also called spindle transfer, is the process of removing the genetic material found in the nucleus of one egg cell, or oocyte, and placing it in another egg that had its nucleus removed. (asu.edu)
  • Also, genetic analysis of the obtained oocytes is needed to confirm their healthy condition. (weebly.com)
  • tissue
  • The availability of various GFP-tagged proteins means that many cellular processes can be monitored in living cells over the course of minutes or hours, and using this technique, processes such as RNP transport, epithelial morphogenesis, and tissue remodeling have been described in great detail in Drosophila oocytes 1,2 . (jove.com)
  • disruption
  • In this study, we showed that resveratrol, a kind of phytoalexin found in the skin of grapes, red wine and other botanical extracts, can alleviate the adverse effects caused by methylglyoxal, such as inhibition of oocyte maturation and disruption of spindle assembly. (jove.com)
  • oogenesis
  • Oogenesis is a limited event which ceases during fetal development with a finite number of oocytes present at birth [ 7 , 10 , 11 ]. (ijbs.com)
  • Cells
  • The formation of oocyte-like cells (OLCs) from somatic stem cells, isolated from newborn mouse skin, has been demonstrated and can be visualized in this video protocol. (jove.com)
  • A centrosome, which consists of two centrioles and the surrounding layers of pericentriolar matrix (PCM) [9- serves as the main microtubule-organizing center (MTOC) during spindle formation in mitotic cells . (deepdyve.com)
  • These cells could then be differentiated into the affected cell type of the patient and studied in vitro, or used to replace the damaged or missing cells. (jove.com)
  • It also offers the potential to store oocytes to rescue endangered species by somatic cell nuclear transfer and for the generation of embryonic stem cells to study development in these species. (warwick.ac.uk)
  • The mature M-II oocyte is one of the largest cells in the human body. (jlgh.org)
  • Shoukhrat Mitalipov, Masahito Tachibana, and their team of researchers replaced the mitochondrial genes of primate embryonic stem cells via spindle transfer. (asu.edu)
  • In a series of experiments between 1960 and 1965, Robert Geoffrey Edwards discovered how to make mammalian egg cells, or oocytes, mature outside of a female's body. (asu.edu)
  • further investigation
  • Further investigation of these processes revealed that methylglyoxal may affect the oocyte quality by resulting in excessive reactive oxygen species production, aberrant mitochondrial distribution and high level lipid peroxidation, and resveratrol can block these cytotoxic changes. (jove.com)
  • reproductive
  • Since October 2012, oocyte cryopreservation has no longer been classified as an "experimental" procedure by the American Society for Reproductive Endocrinology (ASRM), and egg freezing has become available to clinicians. (jlgh.org)
  • The technique was developed by Gianpiero Palermo at the Vrije Universiteit Brussel , in the Center for Reproductive Medicine headed by Paul Devroey and Andre Van Steirteghem . (wikipedia.org)
  • 15 A few oocytes will be released during reproductive life as a consequence of ovulation, and most will be lost as a result of atresia. (aappublications.org)
  • oct4
  • The resulting OLCs express various markers consistent with oocytes such as Oct4 , Vasa , Bmp15 , and Scp3 . (jove.com)
  • prophase
  • Here, we describe methods to isolate prophase-arrested oocytes from mice and for microinjection. (jove.com)
  • mice
  • We report here that mice harboring Hdac1 −/+ / Hdac2 −/− or Hdac2 −/− oocytes are infertile or sub-fertile, respectively. (prolekare.cz)
  • He measured the conditions and timings for in vitro (out of the body) maturation of oocytes from diverse mammals including mice, rats, hamsters, pigs, cows, sheep, and rhesus monkeys, as well as humans. (asu.edu)
  • fertilisation
  • Scientific research within the department is helping to understand the earliest stages of human development from oocyte maturation to the first few days after fertilisation. (ncl.ac.uk)
  • bipolar
  • Bipolar spindles are reorganized by microtubule-based motors including plus-to-minus directed motor dynein and minus-to-plus directed kinesins. (deepdyve.com)