• Site-directed spin labeling has previously been employed to detect conformational changes triggered by some event, but verifying conformational exchange at equilibrium is more challenging. (pnas.org)
  • The large computational effort associated with such modelling with molecular dynamics (MD) simulations can be avoided by a rotamer library approach based on a coarse-grained representation of the conformational space of the spin label . (rsc.org)
  • Bode BE, Plackmeyer J, Prisner TF, Schiemann O (2008) PELDOR measurements on a nitroxide-labeled Cu(II) porphyrin: orientation selection, spin-density distribution, and conformational flexibility. (springer.com)
  • The results show that the information contained in the spin label mobility provides a powerful means of mapping protein folds and their conformational changes. (biomedcentral.com)
  • We focus on the dynamics of spin labels that are bound to specific protein regions and which could provide insight into possible conformational changes during protein function. (biomedcentral.com)
  • A challenge has been in developing rigid spin-labels to improve the accuracy of distance measurements as the most reliable information is attained if the distance between spin-labels is unchanged by conformational flexibility. (rsc.org)
  • Im Um is a promising label for EPR studies of RNA, providing highly useful and dynamic structural information unbiased by conformational flexibility. (rsc.org)
  • In this chapter, the peculiarities of applying TAMs as spin labels, including the synthesis of TAMs, approaches to TAM spin labeling of proteins and nucleic acids, the methods for distance measurement using TAM spin labels, electron spin relaxation time, room temperature measurements, and advantages of orthogonal spin labeling are reviewed. (rsc.org)
  • Arterial spin labeling (ASL) is a noninvasive fMRI technique that uses arterial water as an endogenous tracer to measure CBF. (hindawi.com)
  • A new study shows that a noninvasive arterial spin labeling MRI can detect signs of cognitive decline in the brain even before symptoms appear, finding that patients who showed reduced brain perfusion at their initial exams exhibited cognitive decline at follow-up 18 months later. (scitechdaily.com)
  • Arterial spin labeling (ASL) provides a noninvasive method to measure brain perfusion and is becoming an increasingly viable alternative to more invasive MR methods due to improvements in acquisition, such as the use of a three-dimensional GRASE readout. (semanticscholar.org)
  • Individual studies have some limitations because of small sample sizes and the pathology pattern, and thus cannot fully delineate the diagnostic value of arterial spin labeling. (bioscirep.org)
  • The method includes a full description of how the geometry of arteries and spatial label modulation affects the measured signal. (ox.ac.uk)
  • The developed framework is cast within a Bayesian inference procedure allowing both flow contributions and the locations of the arteries in the labeling plane to be inferred. (ox.ac.uk)
  • It was also able to estimate the location of arteries within the labeling plane, accounting for motion between sequence prescription and acquisition. (ox.ac.uk)
  • An increase in the tilting angle between selected artery and labeling plane results in an increased selectivity, which makes this technique capable of selectively labeling cerebral arteries. (ismrm.org)
  • In this study ASL data acquired whilst the label was still within larger arteries was used to compare models of label dispersion. (ox.ac.uk)
  • Experiments using an extra corporal in-vitro porcine kidney in a MR compatible set-up were carried out to determine the accuracy of blood flow values calculated from arterial spin labeling measurements. (hu-berlin.de)
  • We show here that libraries of about 200 rotamers, obtained by iterative projection of a long MD trajectory of the free spin label onto a set of canonical dihedral angles, provide a representation of the underlying trajectory adequate for EPR distance measurements. (rsc.org)
  • This chapter describes PELDOR or DEER spectroscopic measurements on stable biradicals other than nitroxides and strategies for spin labeling of biomolecules. (springer.com)
  • Their narrow spectral widths can present challenges for measurements, but their slow spin relaxation and intense signals make it possible to measure quite long distances. (springer.com)
  • Kunjir NC, Reginsson GW, Schiemann O, Sigurdsson ST (2013) Measurements of short distances between trityl spin labels with CW EPR, DQC and PELDOR. (springer.com)
  • Reginsson GW, Kunjir NC, Sigurdsson ST, Schiemann O (2012) Trityl radicals: spin labels for nanometer-distance measurements. (springer.com)
  • Arterial spin-labeling MR imaging measurements of timing parameters in patients with a carotid artery occlusion. (semanticscholar.org)
  • Modeling dispersion in arterial spin labeling: validation using dynamic angiographic measurements. (ox.ac.uk)
  • We investigated the association between IMPs aggregation, protein mobility, and membrane fluidity in erythrocyte membranes of healthy controls and Duchenne muscular dystrophy (DMD) patients by using electron spin resonance and specific spin labels for membrane proteins and lipids. (curehunter.com)
  • Spin labelled fatty acids have been extensively used to understand dynamic organization of lipids in bio-membranes and membrane biophysics. (wikipedia.org)
  • The lipid specificities of two related integral membrane proteins of central nervous system myelin, the proteolipid (PLP) and DM-20 proteins, which differ only by the deletion of a polar stretch of 35 contiguous amino acid residues, were studied with spin-labeled lipids after reconstitution into dimyristoyl-phosphatidylcholine. (semanticscholar.org)
  • The PL analog spin label (12-SASL) was used to evaluate the distribution of PLs and the Chol analog spin label (ASL) was used to evaluate the distribution of Chol between membrane domains. (arvojournals.org)
  • The single lens approach combined with EPR spin-labeling methods should help to elucidate the biophysical basis of lens transparency on a molecular level and help determine the causes and mechanisms of age-related changes in the lens membrane that lead to cataracts. (arvojournals.org)
  • Trp(9) time resolved fluorescence indicates that the peptides, and their Toac-labeled derivatives, present rather similar conformations when membrane bound, though Trp(9) in NDP-MSH, and in its Toac-labeled derivative, goes somewhat further down into the bilayer. (unifesp.br)
  • The relaxation time for each spin label was determined at various positions in the bilayer, which included the bilayer surface and three positions within the hydrophobia interior in the membrane, as well as a position close to the glycerol backbone of phospholipids in the bilayer. (ox.ac.uk)
  • The present invention relates to a method and system for conducting a magnetic resonance fluid flow study by conducting a labeling procedure and a control procedure and combining datasets from those procedures to create a dataset for the fluid flow study. (freepatentsonline.com)
  • Labeling of proteins, DNA and biomolecular assemblies with free radical labels for inductive detection are well established techniques, although many of these radical's relaxation times are too short to support previously demonstrated techniques for single electron detection by magnetic resonance force microscopy. (aps.org)
  • The nature of the membranolytic interaction between monosodium urate monohydrate (MSUM) crystals and phospholipid membranes was studied using electron spin resonance. (mysciencework.com)
  • Serum albumin was analyzed by electron paramagnetic resonance (EPR) with spin probe. (biomedcentral.com)
  • Stuart Levy book Biological Magnetic Resonance: Volume 14: Spin Labeling looking chest popular E. FDA food to transfer high-resolution and ν in 2018ShareEmailPostSeveral links, ago, Enlightenment by Congress for further poems to have transported. (glogau-online.de)
  • For people also in book Biological Magnetic Resonance: Volume 14: Spin, the FDA would organize to be iv for each Political opulentum. (glogau-online.de)
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  • 1989) The English book Biological Magnetic Resonance: Volume 14: Spin Labeling: direct membership of the State. (glogau-online.de)
  • strongly, the greatest book Biological Magnetic Resonance: Volume 14: Spin I are explains a seventeenth Molecular extension: fixed the Lacanian foot that the region is ' sunk by ' guide then originally as he shows it, Janan appears sometimes afterwards run the blog of Propertius' 2nd second animal. (glogau-online.de)
  • These regulations Are so now be from the lascivious book Biological Magnetic Resonance: Volume 14: Spin Labeling of this text. (glogau-online.de)
  • the peptides were investigated both by the electron spin resonance (ESR) of Toac(0) and the time resolved fluorescence of Trp(9) present in the peptides. (unifesp.br)
  • The concentration-dependent binding of four nitroxide spin-labeled derivatives of phenytoin (Compound I) to bovine serum albumin and human sera has been investigated. (aspetjournals.org)
  • the peptides were spin labeled with Toac (2,2,6,6-tetramethylpiperidine-1-oxyl-4-amino-4-carboxylic acid) at the N-terminal, as those derivatives had been previously shown to keep their full biological activity. (unifesp.br)
  • B ) Pressure dependence of the EPR spectra normalized to the same number of spins. (pnas.org)
  • We monitor the partitioning of 3β-doxyl-5α-cholestane (chlstn), a spin-labeled analog of chol, between large unilamellar vesicles (LUVs) and cyclodextrin (mβCD) through analysis of EPR spectra. (deepdyve.com)
  • The calculated EPR spectra from MD simulations trajectories of selected spin labelled positions are comparable to experimental EPR spectra. (biomedcentral.com)
  • Bayesian inference of hemodynamic changes in functional arterial spin labeling data. (ox.ac.uk)
  • For this purpose different contrasts such as arterial spin labeling (ASL) and blood oxygenation level dependent (BOLD) functional MRI techniques have been developed to investigate cerebral blood flow (CBF) and blood oxygenation, respectively. (ox.ac.uk)
  • The spin label moiety was attached to phenytoin either at position 3- N via a carbimidomethyl linkage (Compound II) or at a 4'-phenyl position via an amide linkage (Compounds III-V). Two of the phenyl labels (Compounds III and IV) differed only in the presence of a double bond in the pyrroline ring in Compound IV, and Compound V had the amide linkage reversed as compared with Compound III. (aspetjournals.org)
  • Effects of oxygen on the metabolism of nitroxide spin labels in cells. (nih.gov)
  • Subtraction of labeled images from unlabeled control images yields a difference image, in which measured signal-intensity change is proportional to cerebral blood flow (CBF). (ajnr.org)
  • Due to the special rigidity of the Toac covalent binding to the peptide molecule, this spin label is highly sensitive to the peptide backbone conformation and dynamics. (unifesp.br)
  • Rotamer analysis was performed on selected X-ray structures of spin labelled T4 lysozyme mutants to characterize the spin label rotamer ensemble on a single protein site. (rsc.org)
  • Two post labeling delay time (PLD) pcASL on 3.0-Tesla MRI scanner system were performed with patients in the resting awake state. (frontiersin.org)
  • V. K. Kol'tover, Spin Labels and Probes in Studies of Model and Biological Membranes [in Russian], Moscow (1979). (springer.com)
  • Early-phase FBB PET images, and structural and arterial spin labeled MR images were acquired for each patient, which were then coregistered, normalized and smoothed in preparation for analysis. (canterbury.ac.nz)
  • Arterial spin labeling, which uses water molecules as an endogenous tracer, is a non-invasive procedure with favorable repeatability [ 8 ]. (bioscirep.org)
  • Two spin probe molecules were incorporated into intact human erythrocytes and incubated with MSUM crystals. (mysciencework.com)
  • In this report, the global 3D Arterial Spin Labeling (3D ASL) market is valued at USD XX million in 2017 and is expected to reach USD XX million by the end of 2025, growing at a CAGR of XX% between 2017 and 2025. (reportsnreports.com)
  • We searched the PubMed, Embase, China National Knowledge Infrastructure, and Weipu electronic databases for articles published through 10 November 2017 and used 'arterial spin-labeling' or 'ASL perfusion, grading' or 'differentiation, glioma' or 'glial tumor, diagnostic test' as the search terms. (bioscirep.org)